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Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch
Uticaj obrade u autoklavu i hidrolize pululanazom na prinos rezistentnog skroba iz normalnog kukuruznog skroba
dc.creator | Milašinović, Marija | |
dc.creator | Radosavljević, Milica | |
dc.creator | Dokić, Ljubica | |
dc.date.accessioned | 2019-05-16T12:12:12Z | |
dc.date.available | 2019-05-16T12:12:12Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | http://rik.mrizp.rs/handle/123456789/342 | |
dc.description.abstract | In this study, resistant starch (RS), type 3, was prepared by the autoclaving and debranching of normal maize starch isolated from a selected ZP genotype. The objectives of this study were to optimize both starch autoclaving and debranching with pullulanase (PromozymeBrewQ) for the production of RS. Autoclaving at 120°C (30 min) increased the RS content of all samples, whereas freezing at -20°C did not have an obvious effect on the RS contents. The highest RS yield in the autoclaved starch samples was 7.0% after three autoclaving-cooling cycles. After pullulanase debranching at 50°C and retrogradation at 4°C, the RS yields ranged from 10.2 to 25.5 % in all samples (depending on the hydrolysis time). Debranched starch samples with a maximum RS yield of 25.5 % were obtained after a debranching time of 24 h. This study showed that starch from the selected ZP maize genotype is suitable for pullulanase treatment and RS preparation but that additional studies with a greater number of different treatments (incubation time/temperature) are necessary to manipulate and promote crystallization and enhance RS formation. | en |
dc.description.abstract | U ovom radu, rezistentan skrob (RS), tip 3, pripremljen je obradom u autoklavu i hidrolizom sa pululanazom iz normalnog kukuruznog skroba koji je izolovan iz odabranog ZP genotipa kukuruza. Predmet istraživanja je bio da se izvrši optimizacija tretmana obrade u autoklavu i hidrolize pululanazom (PromozymeBrewQ) u cilju dobijanja RS-a. Obrada u autoklavu na 120°C (30 min) je uticala na povećanje sadržaja RS-a u svim uzorcima, dok temperatura čuvanja od -20°C nije imala uticaja na njegov sadržaj. Najveći prinos RS-a u autoklaviranim uzorcima skroba je bio 7,0 % nakon tri ciklusa obrada u autoklavu-hlađenje. Nakon hidrolize pululanazom na 50 °C i retrogradacije na 4°C prinos RS-a u svim uzorcima se kretao od 10,2 do 25,5 % (zavisno od vremena hidrolize). Hidrolizovani uzorci skroba sa maksimalnim prinosom RS-a od 25,5 % određeni su nakon 24 časa inkubacije. Ovo istraživanje pokazuje da je skrob iz odabranog ZP genotipa kukuruza pogodan za hidrolizu pululanazom i dobijanje RS-a, ali su neophodna dalja istraživanja sa većim brojem različitih tretmana (vreme/temperatura inkubacije) u cilju poboljšanja procesa kristalizacije i povećanja prinosa RS-a. | sr |
dc.publisher | Srpsko hemijsko društvo, Beograd | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | maize starch | en |
dc.subject | autoclaving | en |
dc.subject | debranching | en |
dc.subject | pullulanase | en |
dc.subject | resistant starch | en |
dc.title | Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch | en |
dc.title | Uticaj obrade u autoklavu i hidrolize pululanazom na prinos rezistentnog skroba iz normalnog kukuruznog skroba | sr |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.volume | 75 | |
dc.citation.issue | 4 | |
dc.citation.spage | 449 | |
dc.citation.epage | 458 | |
dc.citation.other | 75(4): 449-458 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000277339000004 | |
dc.identifier.doi | 10.2298/JSC090904027M | |
dc.identifier.scopus | 2-s2.0-77951817801 | |
dc.identifier.fulltext | http://rik.mrizp.rs//bitstream/id/2275/340.pdf | |
dc.type.version | publishedVersion |