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Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch

Uticaj obrade u autoklavu i hidrolize pululanazom na prinos rezistentnog skroba iz normalnog kukuruznog skroba

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2010
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Authors
Milašinović, Marija
Radosavljević, Milica
Dokić, Ljubica
Article (Published version)
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Abstract
In this study, resistant starch (RS), type 3, was prepared by the autoclaving and debranching of normal maize starch isolated from a selected ZP genotype. The objectives of this study were to optimize both starch autoclaving and debranching with pullulanase (PromozymeBrewQ) for the production of RS. Autoclaving at 120°C (30 min) increased the RS content of all samples, whereas freezing at -20°C did not have an obvious effect on the RS contents. The highest RS yield in the autoclaved starch samples was 7.0% after three autoclaving-cooling cycles. After pullulanase debranching at 50°C and retrogradation at 4°C, the RS yields ranged from 10.2 to 25.5 % in all samples (depending on the hydrolysis time). Debranched starch samples with a maximum RS yield of 25.5 % were obtained after a debranching time of 24 h. This study showed that starch from the selected ZP maize genotype is suitable for pullulanase treatment and RS preparation but that additional studies with a greater number of differe...nt treatments (incubation time/temperature) are necessary to manipulate and promote crystallization and enhance RS formation.

U ovom radu, rezistentan skrob (RS), tip 3, pripremljen je obradom u autoklavu i hidrolizom sa pululanazom iz normalnog kukuruznog skroba koji je izolovan iz odabranog ZP genotipa kukuruza. Predmet istraživanja je bio da se izvrši optimizacija tretmana obrade u autoklavu i hidrolize pululanazom (PromozymeBrewQ) u cilju dobijanja RS-a. Obrada u autoklavu na 120°C (30 min) je uticala na povećanje sadržaja RS-a u svim uzorcima, dok temperatura čuvanja od -20°C nije imala uticaja na njegov sadržaj. Najveći prinos RS-a u autoklaviranim uzorcima skroba je bio 7,0 % nakon tri ciklusa obrada u autoklavu-hlađenje. Nakon hidrolize pululanazom na 50 °C i retrogradacije na 4°C prinos RS-a u svim uzorcima se kretao od 10,2 do 25,5 % (zavisno od vremena hidrolize). Hidrolizovani uzorci skroba sa maksimalnim prinosom RS-a od 25,5 % određeni su nakon 24 časa inkubacije. Ovo istraživanje pokazuje da je skrob iz odabranog ZP genotipa kukuruza pogodan za hidrolizu pululanazom i dobijanje RS-a, ali su neo...phodna dalja istraživanja sa većim brojem različitih tretmana (vreme/temperatura inkubacije) u cilju poboljšanja procesa kristalizacije i povećanja prinosa RS-a.

Keywords:
maize starch / autoclaving / debranching / pullulanase / resistant starch
Source:
Journal of the Serbian Chemical Society, 2010, 75, 4, 449-458
Publisher:
  • Srpsko hemijsko društvo, Beograd

DOI: 10.2298/JSC090904027M

ISSN: 0352-5139

WoS: 000277339000004

Scopus: 2-s2.0-77951817801
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URI
http://rik.mrizp.rs/handle/123456789/342
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  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Milašinović, Marija
AU  - Radosavljević, Milica
AU  - Dokić, Ljubica
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/342
AB  - In this study, resistant starch (RS), type 3, was prepared by the autoclaving and debranching of normal maize starch isolated from a selected ZP genotype. The objectives of this study were to optimize both starch autoclaving and debranching with pullulanase (PromozymeBrewQ) for the production of RS. Autoclaving at 120°C (30 min) increased the RS content of all samples, whereas freezing at -20°C did not have an obvious effect on the RS contents. The highest RS yield in the autoclaved starch samples was 7.0% after three autoclaving-cooling cycles. After pullulanase debranching at 50°C and retrogradation at 4°C, the RS yields ranged from 10.2 to 25.5 % in all samples (depending on the hydrolysis time). Debranched starch samples with a maximum RS yield of 25.5 % were obtained after a debranching time of 24 h. This study showed that starch from the selected ZP maize genotype is suitable for pullulanase treatment and RS preparation but that additional studies with a greater number of different treatments (incubation time/temperature) are necessary to manipulate and promote crystallization and enhance RS formation.
AB  - U ovom radu, rezistentan skrob (RS), tip 3, pripremljen je obradom u autoklavu i hidrolizom sa pululanazom iz normalnog kukuruznog skroba koji je izolovan iz odabranog ZP genotipa kukuruza. Predmet istraživanja je bio da se izvrši optimizacija tretmana obrade u autoklavu i hidrolize pululanazom (PromozymeBrewQ) u cilju dobijanja RS-a. Obrada u autoklavu na 120°C (30 min) je uticala na povećanje sadržaja RS-a u svim uzorcima, dok temperatura čuvanja od -20°C nije imala uticaja na njegov sadržaj. Najveći prinos RS-a u autoklaviranim uzorcima skroba je bio 7,0 % nakon tri ciklusa obrada u autoklavu-hlađenje. Nakon hidrolize pululanazom na 50 °C i retrogradacije na 4°C prinos RS-a u svim uzorcima se kretao od 10,2 do 25,5 % (zavisno od vremena hidrolize). Hidrolizovani uzorci skroba sa maksimalnim prinosom RS-a od 25,5 % određeni su nakon 24 časa inkubacije. Ovo istraživanje pokazuje da je skrob iz odabranog ZP genotipa kukuruza pogodan za hidrolizu pululanazom i dobijanje RS-a, ali su neophodna dalja istraživanja sa većim brojem različitih tretmana (vreme/temperatura inkubacije) u cilju poboljšanja procesa kristalizacije i povećanja prinosa RS-a.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch
T1  - Uticaj obrade u autoklavu i hidrolize pululanazom na prinos rezistentnog skroba iz normalnog kukuruznog skroba
VL  - 75
IS  - 4
SP  - 449
EP  - 458
DO  - 10.2298/JSC090904027M
UR  - conv_71
ER  - 
@article{
author = "Milašinović, Marija and Radosavljević, Milica and Dokić, Ljubica",
year = "2010",
abstract = "In this study, resistant starch (RS), type 3, was prepared by the autoclaving and debranching of normal maize starch isolated from a selected ZP genotype. The objectives of this study were to optimize both starch autoclaving and debranching with pullulanase (PromozymeBrewQ) for the production of RS. Autoclaving at 120°C (30 min) increased the RS content of all samples, whereas freezing at -20°C did not have an obvious effect on the RS contents. The highest RS yield in the autoclaved starch samples was 7.0% after three autoclaving-cooling cycles. After pullulanase debranching at 50°C and retrogradation at 4°C, the RS yields ranged from 10.2 to 25.5 % in all samples (depending on the hydrolysis time). Debranched starch samples with a maximum RS yield of 25.5 % were obtained after a debranching time of 24 h. This study showed that starch from the selected ZP maize genotype is suitable for pullulanase treatment and RS preparation but that additional studies with a greater number of different treatments (incubation time/temperature) are necessary to manipulate and promote crystallization and enhance RS formation., U ovom radu, rezistentan skrob (RS), tip 3, pripremljen je obradom u autoklavu i hidrolizom sa pululanazom iz normalnog kukuruznog skroba koji je izolovan iz odabranog ZP genotipa kukuruza. Predmet istraživanja je bio da se izvrši optimizacija tretmana obrade u autoklavu i hidrolize pululanazom (PromozymeBrewQ) u cilju dobijanja RS-a. Obrada u autoklavu na 120°C (30 min) je uticala na povećanje sadržaja RS-a u svim uzorcima, dok temperatura čuvanja od -20°C nije imala uticaja na njegov sadržaj. Najveći prinos RS-a u autoklaviranim uzorcima skroba je bio 7,0 % nakon tri ciklusa obrada u autoklavu-hlađenje. Nakon hidrolize pululanazom na 50 °C i retrogradacije na 4°C prinos RS-a u svim uzorcima se kretao od 10,2 do 25,5 % (zavisno od vremena hidrolize). Hidrolizovani uzorci skroba sa maksimalnim prinosom RS-a od 25,5 % određeni su nakon 24 časa inkubacije. Ovo istraživanje pokazuje da je skrob iz odabranog ZP genotipa kukuruza pogodan za hidrolizu pululanazom i dobijanje RS-a, ali su neophodna dalja istraživanja sa većim brojem različitih tretmana (vreme/temperatura inkubacije) u cilju poboljšanja procesa kristalizacije i povećanja prinosa RS-a.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch, Uticaj obrade u autoklavu i hidrolize pululanazom na prinos rezistentnog skroba iz normalnog kukuruznog skroba",
volume = "75",
number = "4",
pages = "449-458",
doi = "10.2298/JSC090904027M",
url = "conv_71"
}
Milašinović, M., Radosavljević, M.,& Dokić, L.. (2010). Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 75(4), 449-458.
https://doi.org/10.2298/JSC090904027M
conv_71
Milašinović M, Radosavljević M, Dokić L. Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch. in Journal of the Serbian Chemical Society. 2010;75(4):449-458.
doi:10.2298/JSC090904027M
conv_71 .
Milašinović, Marija, Radosavljević, Milica, Dokić, Ljubica, "Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch" in Journal of the Serbian Chemical Society, 75, no. 4 (2010):449-458,
https://doi.org/10.2298/JSC090904027M .,
conv_71 .

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