Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
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2010
Authors
Vuletić, MirjanaMarković, Ksenija
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Hadži-Tašković-Šukalović, Vesna
Dragisic-Maksimović, Jelena
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Article (Published version)
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Show full item recordAbstract
The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower ...activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.
Keywords:
antioxidative enzymes / proline / phenolics / Zea mays L.Source:
Biologia Plantarum, 2010, 54, 3, 530-534Publisher:
- Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
Funding / projects:
- Regulacija antioksidativnog metabolizma biljaka u toku rastenja, infekcije patogenima i delovanja abiotičkog stresa: mehanizmi transporta, signalizacije i otpornosti (RS-MESTD-MPN2006-2010-143020)
- Identifikacija izvora tolerantnosti prema suši u gen banci kukuruza (RS-MESTD-MPN2006-2010-20014)
DOI: 10.1007/s10535-010-0093-0
ISSN: 0006-3134