Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
Authorized Users Only
2010
Authors
Vuletić, MirjanaMarković, Ksenija
Hadži-Tašković-Šukalović, Vesna
Dragisic-Maksimović, Jelena
Article (Published version)
Metadata
Show full item recordAbstract
The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower ...activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.
Keywords:
antioxidative enzymes / proline / phenolics / Zea mays L.Source:
Biologia Plantarum, 2010, 54, 3, 530-534Publisher:
- Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
Funding / projects:
- Regulacija antioksidativnog metabolizma biljaka u toku rastenja, infekcije patogenima i delovanja abiotičkog stresa: mehanizmi transporta, signalizacije i otpornosti (RS-MESTD-MPN2006-2010-143020)
- Identifikacija izvora tolerantnosti prema suši u gen banci kukuruza (RS-MESTD-MPN2006-2010-20014)
DOI: 10.1007/s10535-010-0093-0
ISSN: 0006-3134