Application of standard germination and vigour tests for seed quality assessment of maize inbred lines

Abstract

Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field conditions and seed longevity is essential for seed companies. The objective of this work was to evaluate different vigour tests for detecting differences among naturally aged seeds and to assess the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines was detected in seed vigour determined by three tests, in particular by the accelerated ageing test. Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines with Lancaster background. The correlation between the cold test and the accelerated ageing test was significant, while the radical emergence test had a poor correlation with them. Obtained results indicate that the application of either cold test or accelerated ageing test is reliable for screening seed vigour of maize inbred lines.

Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod- nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad- nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad- nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana za skrining vigora semena samooplodnih linija kukuruza