Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.
Само за регистроване кориснике
2008
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively and G(ST) value was 0.360. Genetic distance values calculated using Modif...ied Rogers' Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.
Извор:
Genetika, 2008, 44, 11, 1517-1524Издавач:
- Društvo genetičara Srbije, Beograd
Институција/група
MRIZPTY - JOUR AU - Ignjatović-Micić, Dragana AU - Mladenović Drinić, Snežana AU - Nikolić, Ana AU - Lazić-Jančić, Vesna PY - 2008 UR - http://rik.mrizp.rs/handle/123456789/245 AB - A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers' Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories. VL - 44 IS - 11 SP - 1517 EP - 1524 UR - https://hdl.handle.net/21.15107/rcub_rik_245 ER -
@article{ author = "Ignjatović-Micić, Dragana and Mladenović Drinić, Snežana and Nikolić, Ana and Lazić-Jančić, Vesna", year = "2008", abstract = "A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers' Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.", volume = "44", number = "11", pages = "1517-1524", url = "https://hdl.handle.net/21.15107/rcub_rik_245" }
Ignjatović-Micić, D., Mladenović Drinić, S., Nikolić, A.,& Lazić-Jančić, V.. (2008). Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.. in Genetika Društvo genetičara Srbije, Beograd., 44(11), 1517-1524. https://hdl.handle.net/21.15107/rcub_rik_245
Ignjatović-Micić D, Mladenović Drinić S, Nikolić A, Lazić-Jančić V. Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.. in Genetika. 2008;44(11):1517-1524. https://hdl.handle.net/21.15107/rcub_rik_245 .
Ignjatović-Micić, Dragana, Mladenović Drinić, Snežana, Nikolić, Ana, Lazić-Jančić, Vesna, "Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories." in Genetika, 44, no. 11 (2008):1517-1524, https://hdl.handle.net/21.15107/rcub_rik_245 .