TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Nikolić, Ana
AU  - Stevanović, Milan
AU  - Kandić, Vesna
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2024
UR  - http://rik.mrizp.rs/handle/123456789/1315
AB  - In recent years, countries in Southeast Europe are facing climate changes characterized by extreme hot weather, which contribute to the increased frequency of Aspergillus species. Because of these changes, Aspergillus  parasiticus  was  isolated,  for  the  first  time,  from  maize  grain  in  Serbia (Nikolic et al, 2018). The presence of black powdery mycelia on maize ears indicated occurrence of species of the genus Aspergillus section Nigri, which led to the need for detailed identification of these fungi. Disease incidence ranged from 10 and 15% in August 2013. Maize ears with black powdery symptoms were collected from field in Zemun Polje, Serbia. Symptomatic kernels were surface  sterilized  with  1%  sodium  hypochlorite  solution  for  3  min,  rinsed  three  times  with sterilized  water,  then  incubated  at  25°C  in  the  dark  for  7  days  on  potato  dextrose  agar  (PDA). Twenty  isolates  were  identified  as  genus Aspergillus section Nigri.  Monospore  cultures  formed black cottony colonies with a yellowish border on PDA. The average colony diameter was 50 mm. In  order  to  reliably  identify,  isolates  were  transferred  to  Malt  Extract  agar  (MEA)  and  Czapek Yeast Autolysate agar (CYA) (Samson et al, 2014). On CYA fungal colonies consisted of a white mycelium, covered by a layer of black conidiophores. On MEA fungal colonies were dense, black, with  yellowish  border.  The  reverse  side  was  colorless  to  pale  yellow,  with  a  yellow  ring  in  the middle. The average size of conidia was 4.3 μm. The conidia were globose to sub-globose, smooth to  roughened,  which  coincides  with  previous  research  (Silva  et  al,  2020).  Given  that  the  fungi Aspergillus niger and Aspergillus welwitschiae are morphologically indistinguishable (Susca et al, 2016), species level identification was completed by analysis of a partial sequence of the internal Page 1 of 5transcribed spacer (ITS) region (ITS1/ITS4 primers) and calmodulin gene (CMD5/CMD6 primers) (Samson et al., 2014).  The sequences were compared with the sequences of A. welwitschiae strains registered in the GenBank database based on nucleotide similarity, and results showed 99,64 and 100% similarity with ITS (OL711714) and calmodulin (KX894585), respectively. The sequence was deposited in GenBank with accession numbers OQ456471 (ITS) and OQ426518 (calmodulin). We also confirmed the presence of this species with specific primers (AWEL1/AWEL2) designed by Susca et al. 2020. Pathogenicity test was performed in Zemun Polje on the same maize hybrid from which the fungal species was isolated. Using artificial inoculations by the injecting conidial suspension into the silk channel, three days after 50% of plants reached the silking stage. Twenty ears were inoculated with each isolate, in four replicates (Reid et al, 1996). Inoculum was prepared from 7-day-old colonies on PDA, and 2 ml of a conidial suspension (1×106 spores/ml) was used. Control plants were inoculated with sterile water. All inoculated ears showed symptoms, similar to those from field infections. Control ears were symptomless. The fungus was reisolated and was morphologically  identical  to  the  original  isolates,  thus  completing  Koch’s  postulates.  Based  on molecular,   morphological   and   pathogenic   properties,   the   isolates   were   identified   as A. welwitschiae. This is the first report of A. welwitschiae as the causal agent of black maize ear rot not only in Serbia, but also in the other countries of the Western Balkans. Given that the fungus A. welwitschiae  synthesizes  both  ochratoxin  A  (OTA)  (Battilani  et  al,  2006)  and  fumonisin  (FB) (Frisvad  et  al,  2011),  further  studies  should  be  focused  on  assessment  its  aggressiveness  and toxicological profile.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - First report of Aspergillus welwitschiae causing maize ear rot in Serbia
VL  - 108
IS  - 1
EP  - 209
DO  - 10.1094/PDIS-05-23-0883-PDN
UR  - https://hdl.handle.net/21.15107/rcub_rik_1315
ER  - 

@article{
author = "Nikolić, Milica and Savić, Iva and Nikolić, Ana and Stevanović, Milan and Kandić, Vesna and Stanković, Goran and Stanković, Slavica",
year = "2024",
abstract = "In recent years, countries in Southeast Europe are facing climate changes characterized by extreme hot weather, which contribute to the increased frequency of Aspergillus species. Because of these changes, Aspergillus  parasiticus  was  isolated,  for  the  first  time,  from  maize  grain  in  Serbia (Nikolic et al, 2018). The presence of black powdery mycelia on maize ears indicated occurrence of species of the genus Aspergillus section Nigri, which led to the need for detailed identification of these fungi. Disease incidence ranged from 10 and 15% in August 2013. Maize ears with black powdery symptoms were collected from field in Zemun Polje, Serbia. Symptomatic kernels were surface  sterilized  with  1%  sodium  hypochlorite  solution  for  3  min,  rinsed  three  times  with sterilized  water,  then  incubated  at  25°C  in  the  dark  for  7  days  on  potato  dextrose  agar  (PDA). Twenty  isolates  were  identified  as  genus Aspergillus section Nigri.  Monospore  cultures  formed black cottony colonies with a yellowish border on PDA. The average colony diameter was 50 mm. In  order  to  reliably  identify,  isolates  were  transferred  to  Malt  Extract  agar  (MEA)  and  Czapek Yeast Autolysate agar (CYA) (Samson et al, 2014). On CYA fungal colonies consisted of a white mycelium, covered by a layer of black conidiophores. On MEA fungal colonies were dense, black, with  yellowish  border.  The  reverse  side  was  colorless  to  pale  yellow,  with  a  yellow  ring  in  the middle. The average size of conidia was 4.3 μm. The conidia were globose to sub-globose, smooth to  roughened,  which  coincides  with  previous  research  (Silva  et  al,  2020).  Given  that  the  fungi Aspergillus niger and Aspergillus welwitschiae are morphologically indistinguishable (Susca et al, 2016), species level identification was completed by analysis of a partial sequence of the internal Page 1 of 5transcribed spacer (ITS) region (ITS1/ITS4 primers) and calmodulin gene (CMD5/CMD6 primers) (Samson et al., 2014).  The sequences were compared with the sequences of A. welwitschiae strains registered in the GenBank database based on nucleotide similarity, and results showed 99,64 and 100% similarity with ITS (OL711714) and calmodulin (KX894585), respectively. The sequence was deposited in GenBank with accession numbers OQ456471 (ITS) and OQ426518 (calmodulin). We also confirmed the presence of this species with specific primers (AWEL1/AWEL2) designed by Susca et al. 2020. Pathogenicity test was performed in Zemun Polje on the same maize hybrid from which the fungal species was isolated. Using artificial inoculations by the injecting conidial suspension into the silk channel, three days after 50% of plants reached the silking stage. Twenty ears were inoculated with each isolate, in four replicates (Reid et al, 1996). Inoculum was prepared from 7-day-old colonies on PDA, and 2 ml of a conidial suspension (1×106 spores/ml) was used. Control plants were inoculated with sterile water. All inoculated ears showed symptoms, similar to those from field infections. Control ears were symptomless. The fungus was reisolated and was morphologically  identical  to  the  original  isolates,  thus  completing  Koch’s  postulates.  Based  on molecular,   morphological   and   pathogenic   properties,   the   isolates   were   identified   as A. welwitschiae. This is the first report of A. welwitschiae as the causal agent of black maize ear rot not only in Serbia, but also in the other countries of the Western Balkans. Given that the fungus A. welwitschiae  synthesizes  both  ochratoxin  A  (OTA)  (Battilani  et  al,  2006)  and  fumonisin  (FB) (Frisvad  et  al,  2011),  further  studies  should  be  focused  on  assessment  its  aggressiveness  and toxicological profile.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Aspergillus welwitschiae causing maize ear rot in Serbia",
volume = "108",
number = "1",
pages = "209",
doi = "10.1094/PDIS-05-23-0883-PDN",
url = "https://hdl.handle.net/21.15107/rcub_rik_1315"
}

Nikolić, M., Savić, I., Nikolić, A., Stevanović, M., Kandić, V., Stanković, G.,& Stanković, S.. (2024). First report of Aspergillus welwitschiae causing maize ear rot in Serbia. in Plant Disease
The American Phytopathological Society., 108(1).
https://doi.org/10.1094/PDIS-05-23-0883-PDN
https://hdl.handle.net/21.15107/rcub_rik_1315

Nikolić M, Savić I, Nikolić A, Stevanović M, Kandić V, Stanković G, Stanković S. First report of Aspergillus welwitschiae causing maize ear rot in Serbia. in Plant Disease. 2024;108(1):null-209.
doi:10.1094/PDIS-05-23-0883-PDN
https://hdl.handle.net/21.15107/rcub_rik_1315 .

Nikolić, Milica, Savić, Iva, Nikolić, Ana, Stevanović, Milan, Kandić, Vesna, Stanković, Goran, Stanković, Slavica, "First report of Aspergillus welwitschiae causing maize ear rot in Serbia" in Plant Disease, 108, no. 1 (2024),
https://doi.org/10.1094/PDIS-05-23-0883-PDN .,
https://hdl.handle.net/21.15107/rcub_rik_1315 .

TY  - CONF
AU  - Savić, Iva
AU  - Lucev, Milica
AU  - Nikolić, Ana
AU  - Kandić, Vesna
AU  - Vico, Ivana
AU  - Duduk, Natasa
AU  - Stanković, Slavica
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1326
AB  - Gljive roda Fusarium spadaju u grupu ekonomski značajnih prouzrokovača fuzarioze klasa strnih žita. Vrsta Fusarium verticillioides je kosmopolitski rasprostranjena, ekonomski značajna i toksigena vrsta. Primarni domaćin ove vrste je kukuruz, ali je njeno prisustvo zabeleženo i na pšenici, durum pšenici i ječmu.
Za potrebe izolacije DNK zasejano je 36 izolata Fusarium spp. koji su gajeni na KDA podlozi, 7 dana u termostatu pri temperaturi od 25°C.
Molekularna identifikacija i karakterizacija izvršena je primenom prajmera specifičnih za vrstu F. verticillioides. Specifični prajmeri koji su korišćeni za identifikaciju su VER1/VER2, zasnovani na genu za kalmodulin, i FV-F2/FV-FR, zasnovani na gaoB genu. Odabranih 14 izolata je okarakterisano na osnovu tri regiona: ITS regiona primenom prajmera ITS1 i ITS4. ITS region je univerzalni region za identifikaciju gljiva. Zatim, izolati su okarakterisani i na osnovu delu gena za elongacioni faktor (TEF 1-α regiona) pomoću prajmera EF1/EF2. TEF 1-α region je primarni region za identifikaciju vrste F. verticillioides. Takođe identifikacija je izvršena i na osnovu delu gena za RNK polimerazu (RPB2) primenom 7cf/11ar prajmera. RPB2 region je sekundarni barkod marker za identifikaciju vrste F. verticillioides. Sekvenciranjem tri regiona dobijene su sekvence na osnovu kojih su rekonstruisana filogenetska stabla za svaki region posebno. 
Od 36 izolata, kod 14 izolata dobijeni su amplikoni očekivanih veličina sa prajmerima VER1/VER2 i FV-F2/FV-R. Identifikacija ovih izolata potvrđena je i na osnovu sva tri regiona (ITS, TEF1-α i RPB2).
AB  - Fungi of the genus Fusarium belong to the group of economically important species that cause fusariosis on small grain cereals. The species Fusarium verticillioides is a cosmopolitan, economically important and toxigenic species. The primary host of this species is maize, but its presence has also been detected on wheat, durum wheat and barley.
For the purposes of DNA isolation, 36 isolates of Fusarium spp. were grown on PDA medium, for 7 days in a thermostat at a temperature of 25°C.
Molecular identification and characterization was performed using primers specific for the species F. verticillioides. The specific primers used for identification were VER1/VER2, based on the calmodulin gene, and FV-F2/FV-FR, based on the gaoB gene. The selected 14 isolates were characterized based on three regions: ITS region using primers ITS1 and ITS4. The ITS region is a universal region for fungal identification. Then, the isolates were characterized based on part of the elongation factor gene (TEF 1-α region) using EF1/EF2 primers. The TEF 1-α region is the primary region for identification F. verticillioides. Identification was also performed based on part of the gene for RNA polymerase (RPB2) using the 7cf/11ar primer. The RPB2 region is a secondary barcode marker for species identification of F. verticillioides. By sequencing three regions, sequences were obtained on the basis of which phylogenetic trees were reconstructed for each region separately.
Out of 36 isolates, 14 isolates obtained amplicons of expected sizes with primers VER1/VER2 and FV-F2/FV-R. The identification of these isolates was confirmed based on all three regions (ITS, TEF1-α and RPB2).
PB  - Beograd : Društvo genetičara Srbije
C3  - 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta
T1  - Molekularna identifikacija vrste Fusarium verticillioides patogena zrna strnih žita
T1  - Molecular identification of Fusarium verticillioides pathogen of small grain kernels
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rik_1326
ER  - 

@conference{
author = "Savić, Iva and Lucev, Milica and Nikolić, Ana and Kandić, Vesna and Vico, Ivana and Duduk, Natasa and Stanković, Slavica",
year = "2023",
abstract = "Gljive roda Fusarium spadaju u grupu ekonomski značajnih prouzrokovača fuzarioze klasa strnih žita. Vrsta Fusarium verticillioides je kosmopolitski rasprostranjena, ekonomski značajna i toksigena vrsta. Primarni domaćin ove vrste je kukuruz, ali je njeno prisustvo zabeleženo i na pšenici, durum pšenici i ječmu.
Za potrebe izolacije DNK zasejano je 36 izolata Fusarium spp. koji su gajeni na KDA podlozi, 7 dana u termostatu pri temperaturi od 25°C.
Molekularna identifikacija i karakterizacija izvršena je primenom prajmera specifičnih za vrstu F. verticillioides. Specifični prajmeri koji su korišćeni za identifikaciju su VER1/VER2, zasnovani na genu za kalmodulin, i FV-F2/FV-FR, zasnovani na gaoB genu. Odabranih 14 izolata je okarakterisano na osnovu tri regiona: ITS regiona primenom prajmera ITS1 i ITS4. ITS region je univerzalni region za identifikaciju gljiva. Zatim, izolati su okarakterisani i na osnovu delu gena za elongacioni faktor (TEF 1-α regiona) pomoću prajmera EF1/EF2. TEF 1-α region je primarni region za identifikaciju vrste F. verticillioides. Takođe identifikacija je izvršena i na osnovu delu gena za RNK polimerazu (RPB2) primenom 7cf/11ar prajmera. RPB2 region je sekundarni barkod marker za identifikaciju vrste F. verticillioides. Sekvenciranjem tri regiona dobijene su sekvence na osnovu kojih su rekonstruisana filogenetska stabla za svaki region posebno. 
Od 36 izolata, kod 14 izolata dobijeni su amplikoni očekivanih veličina sa prajmerima VER1/VER2 i FV-F2/FV-R. Identifikacija ovih izolata potvrđena je i na osnovu sva tri regiona (ITS, TEF1-α i RPB2)., Fungi of the genus Fusarium belong to the group of economically important species that cause fusariosis on small grain cereals. The species Fusarium verticillioides is a cosmopolitan, economically important and toxigenic species. The primary host of this species is maize, but its presence has also been detected on wheat, durum wheat and barley.
For the purposes of DNA isolation, 36 isolates of Fusarium spp. were grown on PDA medium, for 7 days in a thermostat at a temperature of 25°C.
Molecular identification and characterization was performed using primers specific for the species F. verticillioides. The specific primers used for identification were VER1/VER2, based on the calmodulin gene, and FV-F2/FV-FR, based on the gaoB gene. The selected 14 isolates were characterized based on three regions: ITS region using primers ITS1 and ITS4. The ITS region is a universal region for fungal identification. Then, the isolates were characterized based on part of the elongation factor gene (TEF 1-α region) using EF1/EF2 primers. The TEF 1-α region is the primary region for identification F. verticillioides. Identification was also performed based on part of the gene for RNA polymerase (RPB2) using the 7cf/11ar primer. The RPB2 region is a secondary barcode marker for species identification of F. verticillioides. By sequencing three regions, sequences were obtained on the basis of which phylogenetic trees were reconstructed for each region separately.
Out of 36 isolates, 14 isolates obtained amplicons of expected sizes with primers VER1/VER2 and FV-F2/FV-R. The identification of these isolates was confirmed based on all three regions (ITS, TEF1-α and RPB2).",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta",
title = "Molekularna identifikacija vrste Fusarium verticillioides patogena zrna strnih žita, Molecular identification of Fusarium verticillioides pathogen of small grain kernels",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_rik_1326"
}

Savić, I., Lucev, M., Nikolić, A., Kandić, V., Vico, I., Duduk, N.,& Stanković, S.. (2023). Molekularna identifikacija vrste Fusarium verticillioides patogena zrna strnih žita. in 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta
Beograd : Društvo genetičara Srbije., 69.
https://hdl.handle.net/21.15107/rcub_rik_1326

Savić I, Lucev M, Nikolić A, Kandić V, Vico I, Duduk N, Stanković S. Molekularna identifikacija vrste Fusarium verticillioides patogena zrna strnih žita. in 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta. 2023;:69.
https://hdl.handle.net/21.15107/rcub_rik_1326 .

Savić, Iva, Lucev, Milica, Nikolić, Ana, Kandić, Vesna, Vico, Ivana, Duduk, Natasa, Stanković, Slavica, "Molekularna identifikacija vrste Fusarium verticillioides patogena zrna strnih žita" in 10. simpozijum Društva selekcionera i semenara Republike Srbije i 7. simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik apstrakta (2023):69,
https://hdl.handle.net/21.15107/rcub_rik_1326 .

TY  - CONF
AU  - Božinović, Sofija
AU  - Vančetović, Jelena
AU  - Djordjevic Melnik, Olivera
AU  - Nikolić, Ana
AU  - Pavlov, Jovan
AU  - Čamdžija, Zoran
AU  - Grčić, Nikola
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1278
AB  - In vivo metoda dupliciranih haploida u Institutu za kukuruz „Zemun Polje“ koristi se u oplemenjivanju kukuruza od 2014. godine. Pristup omogućava dobijanje potpuno homozigotnih inbred linija tzv. duplohaploidnih (DH) linija za samo dve godine (dve do tri generacije), za razliku od konvencionalnog oplemenjivanja gde je potrebno bar sedam do osam generacija kako bi se postigla neophodna homozigotnost. Metoda se sastoji iz nekoliko koraka: 1) indukcija haploida iz odabranih populacija ukrštanjem sa linijom induktorom haploida (prva godina/letnja generacija); 2) selekcija haploidnih zrna (prva godina/letnja generacija); 3) duplikacija hromozoma (druga godina/zimska generacija) i 4) umnožavanje dobijenih DH linija (druga godina/letnja generacija). Do sada je dobijeno oko 13000 DH linija iz oko 350 populacija koje pripadaju različitim heterotičnim grupama. U okviru programa sprovode se i aktivnosti sa ciljem razvoja novih induktora haploida sa povišenim procentom indukcije haploida i poboljšanim agronomskim svojstvima. Osam induktora-kandidata je specifičnom procesom selekcije odabrano i u 2023. godini ocenjeno u mini ogledu sa komercijalno dostupnim induktorima, a mereni su visina biljke, produkcija polena, ozrnjenost u ukrštanju, kao i procenat indukcije haploida. U okviru rada na optimizaciji metode, ispitivan je i uticaj populacije (heterotične grupe) i spoljašnje sredine na uspešnost metode. Utvrđen je značajan uticaj spoljašnjih uslova, kao i heterotične grupe odnosno genotipa na indukciju i duplikaciju, kao i konačnu uspešnost metode. Prvi hibridi sa DH linijom kao roditeljskom komponentom već su registrovani od strane Sortne komisije Republike Srbije za komercijalno gajenje u Srbiji.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
C3  - X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta
T1  - Oplemenjivanje kukuruza uz pomoć metode dupliciranih haploida u Institutu za kukuruz “Zemun Polje”
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_rik_1278
ER  - 

@conference{
author = "Božinović, Sofija and Vančetović, Jelena and Djordjevic Melnik, Olivera and Nikolić, Ana and Pavlov, Jovan and Čamdžija, Zoran and Grčić, Nikola",
year = "2023",
abstract = "In vivo metoda dupliciranih haploida u Institutu za kukuruz „Zemun Polje“ koristi se u oplemenjivanju kukuruza od 2014. godine. Pristup omogućava dobijanje potpuno homozigotnih inbred linija tzv. duplohaploidnih (DH) linija za samo dve godine (dve do tri generacije), za razliku od konvencionalnog oplemenjivanja gde je potrebno bar sedam do osam generacija kako bi se postigla neophodna homozigotnost. Metoda se sastoji iz nekoliko koraka: 1) indukcija haploida iz odabranih populacija ukrštanjem sa linijom induktorom haploida (prva godina/letnja generacija); 2) selekcija haploidnih zrna (prva godina/letnja generacija); 3) duplikacija hromozoma (druga godina/zimska generacija) i 4) umnožavanje dobijenih DH linija (druga godina/letnja generacija). Do sada je dobijeno oko 13000 DH linija iz oko 350 populacija koje pripadaju različitim heterotičnim grupama. U okviru programa sprovode se i aktivnosti sa ciljem razvoja novih induktora haploida sa povišenim procentom indukcije haploida i poboljšanim agronomskim svojstvima. Osam induktora-kandidata je specifičnom procesom selekcije odabrano i u 2023. godini ocenjeno u mini ogledu sa komercijalno dostupnim induktorima, a mereni su visina biljke, produkcija polena, ozrnjenost u ukrštanju, kao i procenat indukcije haploida. U okviru rada na optimizaciji metode, ispitivan je i uticaj populacije (heterotične grupe) i spoljašnje sredine na uspešnost metode. Utvrđen je značajan uticaj spoljašnjih uslova, kao i heterotične grupe odnosno genotipa na indukciju i duplikaciju, kao i konačnu uspešnost metode. Prvi hibridi sa DH linijom kao roditeljskom komponentom već su registrovani od strane Sortne komisije Republike Srbije za komercijalno gajenje u Srbiji.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta",
title = "Oplemenjivanje kukuruza uz pomoć metode dupliciranih haploida u Institutu za kukuruz “Zemun Polje”",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_rik_1278"
}

Božinović, S., Vančetović, J., Djordjevic Melnik, O., Nikolić, A., Pavlov, J., Čamdžija, Z.,& Grčić, N.. (2023). Oplemenjivanje kukuruza uz pomoć metode dupliciranih haploida u Institutu za kukuruz “Zemun Polje”. in X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta
Beograd : Društvo genetičara Srbije., 85-85.
https://hdl.handle.net/21.15107/rcub_rik_1278

Božinović S, Vančetović J, Djordjevic Melnik O, Nikolić A, Pavlov J, Čamdžija Z, Grčić N. Oplemenjivanje kukuruza uz pomoć metode dupliciranih haploida u Institutu za kukuruz “Zemun Polje”. in X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta. 2023;:85-85.
https://hdl.handle.net/21.15107/rcub_rik_1278 .

Božinović, Sofija, Vančetović, Jelena, Djordjevic Melnik, Olivera, Nikolić, Ana, Pavlov, Jovan, Čamdžija, Zoran, Grčić, Nikola, "Oplemenjivanje kukuruza uz pomoć metode dupliciranih haploida u Institutu za kukuruz “Zemun Polje”" in X Simpozijum Društva selekcionera i semenara Republike Srbije i VII Simpozijuma Sekcije za oplemenjivanje organizama Društva genetičara Srbija, Vrnjačka Banja, 16-18. oktobar 2023, Zbornik abstrakta (2023):85-85,
https://hdl.handle.net/21.15107/rcub_rik_1278 .

TY  - PAT
AU  - Grčić, Nikola
AU  - Filipović, Milomir
AU  - Nikolić, Ana
AU  - Mladenović, Marko
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1267
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-09-02434/2/2021-11 od 30.03.2023. godine
T1  - ZP 6049
UR  - https://hdl.handle.net/21.15107/rcub_rik_1267
ER  - 

@misc{
author = "Grčić, Nikola and Filipović, Milomir and Nikolić, Ana and Mladenović, Marko",
year = "2023",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-09-02434/2/2021-11 od 30.03.2023. godine",
title = "ZP 6049",
url = "https://hdl.handle.net/21.15107/rcub_rik_1267"
}

Grčić, N., Filipović, M., Nikolić, A.,& Mladenović, M.. (2023). ZP 6049. in Rešenje broj 320-09-02434/2/2021-11 od 30.03.2023. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1267

Grčić N, Filipović M, Nikolić A, Mladenović M. ZP 6049. in Rešenje broj 320-09-02434/2/2021-11 od 30.03.2023. godine. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1267 .

Grčić, Nikola, Filipović, Milomir, Nikolić, Ana, Mladenović, Marko, "ZP 6049" in Rešenje broj 320-09-02434/2/2021-11 od 30.03.2023. godine (2023),
https://hdl.handle.net/21.15107/rcub_rik_1267 .

TY  - PAT
AU  - Stevanović, Milan
AU  - Delić, Nenad
AU  - Nikolić, Ana
AU  - Filipović, Milomir
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1250
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine
T1  - ZP 5255
UR  - https://hdl.handle.net/21.15107/rcub_rik_1250
ER  - 

@misc{
author = "Stevanović, Milan and Delić, Nenad and Nikolić, Ana and Filipović, Milomir",
year = "2023",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine",
title = "ZP 5255",
url = "https://hdl.handle.net/21.15107/rcub_rik_1250"
}

Stevanović, M., Delić, N., Nikolić, A.,& Filipović, M.. (2023). ZP 5255. in Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1250

Stevanović M, Delić N, Nikolić A, Filipović M. ZP 5255. in Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1250 .

Stevanović, Milan, Delić, Nenad, Nikolić, Ana, Filipović, Milomir, "ZP 5255" in Rešenje broj 320-09-02448/2/2021-11 od 30.03.2023. godine (2023),
https://hdl.handle.net/21.15107/rcub_rik_1250 .

TY  - GEN
AU  - Milivojević, Marija
AU  - Branković-Radojčić, Dragana
AU  - Petrović, Tanja
AU  - Nikolić, Ana
AU  - Marković, Ksenija
AU  - Srdić, Jelena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1283
AB  - Ovim tehničkim rešenjem opisan je metod ispitivanja vigora semena kukuruza hladnim testom. Postupak podrazumeva izlaganje semena stresnim uslovima za klijanje i nicanje kako bi se stekao bolji uvid o potencijalnom ponašanju i nicanju kukuruza u polju. Hladni test je pogodan za ispitivanje vigora jer istovremeno uključuje nekoliko stresnih faktora: temperaturu, povećanu vlažnost supstrata i prisustvo patogena. Prva faza ispitivanja se odvija na niskim temperaturama (7 dana na 10 °C) nakon čeka se uzorci prebacuju u optimalne uslove za rast (7 dana na naizmeničnim temperaturama 20/30 °C). Primena hladnog testa doprinosi sveobuhvatnom pristupu ispitivanja kvaliteta semena kukuruza. Dobijaju se dodatne informacije o ponašanju semena u suboptimalnim uslovima za klijanje i nicanje kukuruza što je od značaja i za proizvođače semena, i za krajnje korisnike. Ispitivanjem vigora semena mogu se identifikovati visoko kvalitetne partije semena koje bolje podnose stresne uslove u polju i mogu se sejati ranije u proleće, i nasuprot njima one slabo vigorozne partije semena koje bi trebalo sejati u optimalnijim uslovima. Rana setva kukuruza omogućava duži vegetacioni period čime su obezbeđeni uslovi za veće i stabilnije prinose i veća je mogućnost izbegavanja letnje suše. Osim tolerantnosti na niske temperature vigor ukazuje i na mogućnost postizanja željenog sklopa useva što je od izuzetne važnosti za postizanje visokih prinosa. Laboratorija za ispitivanje semena Instituta za kukuruz „Zemun Polje“ sprovela je standardizaciju uslova ispitivanja i optimizaciju metode hladnog testa i jedina je laboratorija sa ovim testom u svom obimu akreditacije na nacionalnom nivou.
PB  - Beograd : Matični naučni odbor za biologiju i poljoprivredu
T2  - 8. redovna sednica Matičnog naučnog odbora za biologiju i poljoprivredu - Potvrda
T1  - Hladni test – Cold test, akreditovana metoda za ispitivanje vigora semena kukuruza
UR  - https://hdl.handle.net/21.15107/rcub_rik_1283
ER  - 

@misc{
author = "Milivojević, Marija and Branković-Radojčić, Dragana and Petrović, Tanja and Nikolić, Ana and Marković, Ksenija and Srdić, Jelena",
year = "2022",
abstract = "Ovim tehničkim rešenjem opisan je metod ispitivanja vigora semena kukuruza hladnim testom. Postupak podrazumeva izlaganje semena stresnim uslovima za klijanje i nicanje kako bi se stekao bolji uvid o potencijalnom ponašanju i nicanju kukuruza u polju. Hladni test je pogodan za ispitivanje vigora jer istovremeno uključuje nekoliko stresnih faktora: temperaturu, povećanu vlažnost supstrata i prisustvo patogena. Prva faza ispitivanja se odvija na niskim temperaturama (7 dana na 10 °C) nakon čeka se uzorci prebacuju u optimalne uslove za rast (7 dana na naizmeničnim temperaturama 20/30 °C). Primena hladnog testa doprinosi sveobuhvatnom pristupu ispitivanja kvaliteta semena kukuruza. Dobijaju se dodatne informacije o ponašanju semena u suboptimalnim uslovima za klijanje i nicanje kukuruza što je od značaja i za proizvođače semena, i za krajnje korisnike. Ispitivanjem vigora semena mogu se identifikovati visoko kvalitetne partije semena koje bolje podnose stresne uslove u polju i mogu se sejati ranije u proleće, i nasuprot njima one slabo vigorozne partije semena koje bi trebalo sejati u optimalnijim uslovima. Rana setva kukuruza omogućava duži vegetacioni period čime su obezbeđeni uslovi za veće i stabilnije prinose i veća je mogućnost izbegavanja letnje suše. Osim tolerantnosti na niske temperature vigor ukazuje i na mogućnost postizanja željenog sklopa useva što je od izuzetne važnosti za postizanje visokih prinosa. Laboratorija za ispitivanje semena Instituta za kukuruz „Zemun Polje“ sprovela je standardizaciju uslova ispitivanja i optimizaciju metode hladnog testa i jedina je laboratorija sa ovim testom u svom obimu akreditacije na nacionalnom nivou.",
publisher = "Beograd : Matični naučni odbor za biologiju i poljoprivredu",
journal = "8. redovna sednica Matičnog naučnog odbora za biologiju i poljoprivredu - Potvrda",
title = "Hladni test – Cold test, akreditovana metoda za ispitivanje vigora semena kukuruza",
url = "https://hdl.handle.net/21.15107/rcub_rik_1283"
}

Milivojević, M., Branković-Radojčić, D., Petrović, T., Nikolić, A., Marković, K.,& Srdić, J.. (2022). Hladni test – Cold test, akreditovana metoda za ispitivanje vigora semena kukuruza. in 8. redovna sednica Matičnog naučnog odbora za biologiju i poljoprivredu - Potvrda
Beograd : Matični naučni odbor za biologiju i poljoprivredu..
https://hdl.handle.net/21.15107/rcub_rik_1283

Milivojević M, Branković-Radojčić D, Petrović T, Nikolić A, Marković K, Srdić J. Hladni test – Cold test, akreditovana metoda za ispitivanje vigora semena kukuruza. in 8. redovna sednica Matičnog naučnog odbora za biologiju i poljoprivredu - Potvrda. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1283 .

Milivojević, Marija, Branković-Radojčić, Dragana, Petrović, Tanja, Nikolić, Ana, Marković, Ksenija, Srdić, Jelena, "Hladni test – Cold test, akreditovana metoda za ispitivanje vigora semena kukuruza" in 8. redovna sednica Matičnog naučnog odbora za biologiju i poljoprivredu - Potvrda (2022),
https://hdl.handle.net/21.15107/rcub_rik_1283 .

TY  - JOUR
AU  - Savić, Iva
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Kandić, Vesna
AU  - Vico, Ivana
AU  - Duduk, Natasa
AU  - Stanković, Slavica
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1301
AB  - Triticale (Triticosecale Wittmack) is a cereal crop species developed to combine positive traits of wheat and rye into a single plant (Arendt and Zannini 2013). Due to its high protein content, favorable amino acid composition, and nutritional values higher than maize, triticale is an excellent component for preparing feed for domestic animals (Đekić et al. 2019). In May 2017, discolored spikes of triticale were observed in a field in Zemun Polje, Serbia, with a disease incidence between 15 and 20%. In total, 400 kernels obtained from discolored spikes were surface sterilized with 1% sodium hypochlorite solution for 3 min, then rinsed three times with sterilized water, plated on potato dextrose agar (PDA) at 25°C, and incubated in the dark for 7 days. In total, 15 isolates of genus Fusarium on PDA formed cottony white to greyish-purple colonies with dark yellow to purple-gray reverse and average colonies. Obtained isolates were transferred to carnation leaf agar (CLA) and synthetic nutrition agar (SNA) for a 7-day incubation in the dark at 25°C. Ten isolates formed “rabbit ear” monophialides, which is the main characteristic of Fusarium verticillioides (Sacc.) Nirenberg. Microconidia are formed from monophialids in long chains. Their shape is oval with a flattened base, without septa (4.0 to 18.0 by 1.5 to 4.5 µm). The shape of macroconidia is slightly falcate to almost straight (3.5 to 4.5 by 31 to 58 µm). Chlamydospores are not produced (Leslie and Summerell 2006). The genomic DNA from one F. verticillioides isolate was extracted using the DNeasy Plant Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. Molecular identification was confirmed by sequencing the internal transcribed spacer (ITS), translation elongation factor 1α (EF-1α), and RPB2 (O’Donnell et al. 2000). The sequence was deposited in GenBank as MZ664391, MZ666958, and MZ666957. This sequence was compared with the sequences of F. verticillioides strains registered in the GenBank database based on nucleotide similarity. It showed 100% identity to the sequences MT180471.1 (ITS), MN861767.1 (EF-1α), and MT264836.1 (RPB2). Pathogenicity of all isolates was tested on 20 randomly selected triticale spikes in four replicates (Mesterházy et al. 1999). Inoculation was performed when half of the plants reached the flowering stage by spraying with 20 ml of spore suspension (1 × 106 spores/ml from 7-day-old cultures on PDA). F. verticillioides RBG 1603 Q27 was used as a positive control. Inoculated spikes were covered with PVC bags for 48 h. Discolored spikes were observed after 3 weeks of incubation in a greenhouse at 20°C with a photoperiod of 16 and 8 h (light and dark). All inoculated spikes showed symptoms, similar to those from field infections. Control spikes were symptomless. The fungus was reisolated and was morphologically identical to the original isolates, thus completing Koch’s postulates. Based on morphological, molecular, and pathogenic features, the isolated fungus was identified as F. verticillioides. Fusarium spp. infect crop plants worldwide, resulting in yield loss and reduced grain quality due to mycotoxin contamination (Asam et al. 2017). In Serbia, F. verticillioides was isolated from wheat and barley grains (Stanković et al. 2012) and, to the best of our knowledge, this is the first report of F. verticillioides on triticale grain in Serbia.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - First report of Fusarium verticillioides causing fusariosis on triticale grain in Serbia
VL  - 106
IS  - 3
SP  - 1071
UR  - https://hdl.handle.net/21.15107/rcub_rik_1301
ER  - 

@article{
author = "Savić, Iva and Nikolić, Milica and Nikolić, Ana and Kandić, Vesna and Vico, Ivana and Duduk, Natasa and Stanković, Slavica",
year = "2022",
abstract = "Triticale (Triticosecale Wittmack) is a cereal crop species developed to combine positive traits of wheat and rye into a single plant (Arendt and Zannini 2013). Due to its high protein content, favorable amino acid composition, and nutritional values higher than maize, triticale is an excellent component for preparing feed for domestic animals (Đekić et al. 2019). In May 2017, discolored spikes of triticale were observed in a field in Zemun Polje, Serbia, with a disease incidence between 15 and 20%. In total, 400 kernels obtained from discolored spikes were surface sterilized with 1% sodium hypochlorite solution for 3 min, then rinsed three times with sterilized water, plated on potato dextrose agar (PDA) at 25°C, and incubated in the dark for 7 days. In total, 15 isolates of genus Fusarium on PDA formed cottony white to greyish-purple colonies with dark yellow to purple-gray reverse and average colonies. Obtained isolates were transferred to carnation leaf agar (CLA) and synthetic nutrition agar (SNA) for a 7-day incubation in the dark at 25°C. Ten isolates formed “rabbit ear” monophialides, which is the main characteristic of Fusarium verticillioides (Sacc.) Nirenberg. Microconidia are formed from monophialids in long chains. Their shape is oval with a flattened base, without septa (4.0 to 18.0 by 1.5 to 4.5 µm). The shape of macroconidia is slightly falcate to almost straight (3.5 to 4.5 by 31 to 58 µm). Chlamydospores are not produced (Leslie and Summerell 2006). The genomic DNA from one F. verticillioides isolate was extracted using the DNeasy Plant Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. Molecular identification was confirmed by sequencing the internal transcribed spacer (ITS), translation elongation factor 1α (EF-1α), and RPB2 (O’Donnell et al. 2000). The sequence was deposited in GenBank as MZ664391, MZ666958, and MZ666957. This sequence was compared with the sequences of F. verticillioides strains registered in the GenBank database based on nucleotide similarity. It showed 100% identity to the sequences MT180471.1 (ITS), MN861767.1 (EF-1α), and MT264836.1 (RPB2). Pathogenicity of all isolates was tested on 20 randomly selected triticale spikes in four replicates (Mesterházy et al. 1999). Inoculation was performed when half of the plants reached the flowering stage by spraying with 20 ml of spore suspension (1 × 106 spores/ml from 7-day-old cultures on PDA). F. verticillioides RBG 1603 Q27 was used as a positive control. Inoculated spikes were covered with PVC bags for 48 h. Discolored spikes were observed after 3 weeks of incubation in a greenhouse at 20°C with a photoperiod of 16 and 8 h (light and dark). All inoculated spikes showed symptoms, similar to those from field infections. Control spikes were symptomless. The fungus was reisolated and was morphologically identical to the original isolates, thus completing Koch’s postulates. Based on morphological, molecular, and pathogenic features, the isolated fungus was identified as F. verticillioides. Fusarium spp. infect crop plants worldwide, resulting in yield loss and reduced grain quality due to mycotoxin contamination (Asam et al. 2017). In Serbia, F. verticillioides was isolated from wheat and barley grains (Stanković et al. 2012) and, to the best of our knowledge, this is the first report of F. verticillioides on triticale grain in Serbia.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Fusarium verticillioides causing fusariosis on triticale grain in Serbia",
volume = "106",
number = "3",
pages = "1071",
url = "https://hdl.handle.net/21.15107/rcub_rik_1301"
}

Savić, I., Nikolić, M., Nikolić, A., Kandić, V., Vico, I., Duduk, N.,& Stanković, S.. (2022). First report of Fusarium verticillioides causing fusariosis on triticale grain in Serbia. in Plant Disease
The American Phytopathological Society., 106(3), 1071.
https://hdl.handle.net/21.15107/rcub_rik_1301

Savić I, Nikolić M, Nikolić A, Kandić V, Vico I, Duduk N, Stanković S. First report of Fusarium verticillioides causing fusariosis on triticale grain in Serbia. in Plant Disease. 2022;106(3):1071.
https://hdl.handle.net/21.15107/rcub_rik_1301 .

Savić, Iva, Nikolić, Milica, Nikolić, Ana, Kandić, Vesna, Vico, Ivana, Duduk, Natasa, Stanković, Slavica, "First report of Fusarium verticillioides causing fusariosis on triticale grain in Serbia" in Plant Disease, 106, no. 3 (2022):1071,
https://hdl.handle.net/21.15107/rcub_rik_1301 .

TY  - CONF
AU  - Božinović, Sofija
AU  - Djordjevic Melnik, Olivera
AU  - Čamdžija, Zoran
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1288
AB  - Red and blue colors in maize kernel are the consequence of anthocyanin pigments, responsible for the increased phenolic content and antioxidant activity in maize. Therefore, interest in pigmented maize as a novel functional food due to its possible health benefits and unique taste has grown. The red color in maize kernel originates from pericarp and is maternally inherited while the blue color originates from aleurone, which is triploid in nature and its mode of inheritance is more complicated. Red and blue maize are usually low-yielding populations, and at the Maize Research Institute Zemun Polje (MRIZP), commercial inbred lines have been converted to red and blue colors to produce high-yielding red and blue maize hybrids.
Maize extractable phenolic content (EPC) can be directly determined through wet chemistry and measures phenolic amides, glycerides, free phenolic acids, and some flavonoids. In this study, however, Fourier-transformed near-infrared reflectance spectroscopy (FT-NIRS), as a non-destructive, inexpensive, and rapid technique has been used to scan EPC profiles of 56 MRIZP maize samples (14 genotypes in two replications and two environments) differing in kernel color: yellow, red pericarp, blue aleurone and red pericarp with blue aleurone. Twenty samples with outlying EPC values have been directly analyzed for total phenolics and these values have been used to adjust and improve NIRS calibration.
EPC values ranged from 1.69 to 6.82 mg g-1 across genotypes and environments. Two red inbred lines had the highest EPC content in 2017, 6.82 and 5.11 mg g-1, and, overall samples from 2017 had a higher content of EPC compared to the same genotypes grown in 2018. Red genotypes on average had the highest EPC content, followed by red-blue, while yellow and blue kernels had similar and the lowest content within the samples.
PB  - Belgrade : Maize Research Institute Zemun Polje
C3  - XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts
T1  - Estimation of phenolic content in yellow, red, blue and red-blue maize by near-infrared reflectance spectroscopy
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rik_1288
ER  - 

@conference{
author = "Božinović, Sofija and Djordjevic Melnik, Olivera and Čamdžija, Zoran and Nikolić, Ana and Kostadinović, Marija and Vančetović, Jelena",
year = "2022",
abstract = "Red and blue colors in maize kernel are the consequence of anthocyanin pigments, responsible for the increased phenolic content and antioxidant activity in maize. Therefore, interest in pigmented maize as a novel functional food due to its possible health benefits and unique taste has grown. The red color in maize kernel originates from pericarp and is maternally inherited while the blue color originates from aleurone, which is triploid in nature and its mode of inheritance is more complicated. Red and blue maize are usually low-yielding populations, and at the Maize Research Institute Zemun Polje (MRIZP), commercial inbred lines have been converted to red and blue colors to produce high-yielding red and blue maize hybrids.
Maize extractable phenolic content (EPC) can be directly determined through wet chemistry and measures phenolic amides, glycerides, free phenolic acids, and some flavonoids. In this study, however, Fourier-transformed near-infrared reflectance spectroscopy (FT-NIRS), as a non-destructive, inexpensive, and rapid technique has been used to scan EPC profiles of 56 MRIZP maize samples (14 genotypes in two replications and two environments) differing in kernel color: yellow, red pericarp, blue aleurone and red pericarp with blue aleurone. Twenty samples with outlying EPC values have been directly analyzed for total phenolics and these values have been used to adjust and improve NIRS calibration.
EPC values ranged from 1.69 to 6.82 mg g-1 across genotypes and environments. Two red inbred lines had the highest EPC content in 2017, 6.82 and 5.11 mg g-1, and, overall samples from 2017 had a higher content of EPC compared to the same genotypes grown in 2018. Red genotypes on average had the highest EPC content, followed by red-blue, while yellow and blue kernels had similar and the lowest content within the samples.",
publisher = "Belgrade : Maize Research Institute Zemun Polje",
journal = "XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts",
title = "Estimation of phenolic content in yellow, red, blue and red-blue maize by near-infrared reflectance spectroscopy",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_rik_1288"
}

Božinović, S., Djordjevic Melnik, O., Čamdžija, Z., Nikolić, A., Kostadinović, M.,& Vančetović, J.. (2022). Estimation of phenolic content in yellow, red, blue and red-blue maize by near-infrared reflectance spectroscopy. in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts
Belgrade : Maize Research Institute Zemun Polje., 69-69.
https://hdl.handle.net/21.15107/rcub_rik_1288

Božinović S, Djordjevic Melnik O, Čamdžija Z, Nikolić A, Kostadinović M, Vančetović J. Estimation of phenolic content in yellow, red, blue and red-blue maize by near-infrared reflectance spectroscopy. in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts. 2022;:69-69.
https://hdl.handle.net/21.15107/rcub_rik_1288 .

Božinović, Sofija, Djordjevic Melnik, Olivera, Čamdžija, Zoran, Nikolić, Ana, Kostadinović, Marija, Vančetović, Jelena, "Estimation of phenolic content in yellow, red, blue and red-blue maize by near-infrared reflectance spectroscopy" in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts (2022):69-69,
https://hdl.handle.net/21.15107/rcub_rik_1288 .

TY  - CONF
AU  - Božinović, Sofija
AU  - Dodig, Dejan
AU  - Nikolić, Ana
AU  - Zorić, Miroslav
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Weigelt-Fischer, Kathleen
AU  - Altmann, Thomas
AU  - Junker, Astrid
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1286
AB  - Limited water availability and nitrogen deficiency are the most restricting factors for maize growth. Although maize susceptibility to drought is the highest at the reproductive stage, water shortage during vegetative development can significantly reduce grain yield. The aim of this study was to assess the genetic variation of growth dynamics in 20 maize inbred lines (ILs) through automated phenotyping based on visible light (RGB) imaging in a greenhouse under optimal (C), nitrogen (N), water (W), and combined nitrogen and water stress (NW) conditions. Thirteen biomass-related and morpho-physiological image-derived traits acquired at 33 time points were selected, covering developmental stages from five fully developed leaves to 10-13 fully developed leaves, depending on treatment. Estimated biomass volume (EBv), a proxy for biomass in our study, showed to be the most sensitive image-derived morphological trait to water and combined water and nitrogen stress with the maximum reduction of 53 and 54% in W and NW compared to C at the time of maximum water stress. Genotypic variation of EBv within each treatment was consistently high and over 80%, while the analysis across treatments showed that as stress got higher the genetic variation got lower (32-38%) and the interaction term became more prominent. Phenotypic correlations between EBv and other image-derived traits at early stages showed that under no stress or mild stress conditions, morphological traits were more appropriate than color-related traits for the prediction of biomass accumulation, while under more severe stress conditions, color-related traits and chlorophyll fluorescence are more useful to differentiate genotypes for high biomass. To investigate whether ILs can express EBv in similar patterns, temporal profiles were clustered by using the fuzzy c-means clustering algorithm, and two temporal dynamics of EBv patterns among the studied ILs in each treatment were identified.
C3  - 7th International Plant Phenotyping Symposium ‘Plant Phenotyping for a Sustainable Future’, Wageningen, the Netherlands September 26-30, 2022, IPPS 2022 Conference Book
T1  - High-throughput phenotyping of maize growth dynamics under nitrogen and water stress
SP  - 95
EP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rik_1286
ER  - 

@conference{
author = "Božinović, Sofija and Dodig, Dejan and Nikolić, Ana and Zorić, Miroslav and Vančetović, Jelena and Ignjatović-Micić, Dragana and Weigelt-Fischer, Kathleen and Altmann, Thomas and Junker, Astrid",
year = "2022",
abstract = "Limited water availability and nitrogen deficiency are the most restricting factors for maize growth. Although maize susceptibility to drought is the highest at the reproductive stage, water shortage during vegetative development can significantly reduce grain yield. The aim of this study was to assess the genetic variation of growth dynamics in 20 maize inbred lines (ILs) through automated phenotyping based on visible light (RGB) imaging in a greenhouse under optimal (C), nitrogen (N), water (W), and combined nitrogen and water stress (NW) conditions. Thirteen biomass-related and morpho-physiological image-derived traits acquired at 33 time points were selected, covering developmental stages from five fully developed leaves to 10-13 fully developed leaves, depending on treatment. Estimated biomass volume (EBv), a proxy for biomass in our study, showed to be the most sensitive image-derived morphological trait to water and combined water and nitrogen stress with the maximum reduction of 53 and 54% in W and NW compared to C at the time of maximum water stress. Genotypic variation of EBv within each treatment was consistently high and over 80%, while the analysis across treatments showed that as stress got higher the genetic variation got lower (32-38%) and the interaction term became more prominent. Phenotypic correlations between EBv and other image-derived traits at early stages showed that under no stress or mild stress conditions, morphological traits were more appropriate than color-related traits for the prediction of biomass accumulation, while under more severe stress conditions, color-related traits and chlorophyll fluorescence are more useful to differentiate genotypes for high biomass. To investigate whether ILs can express EBv in similar patterns, temporal profiles were clustered by using the fuzzy c-means clustering algorithm, and two temporal dynamics of EBv patterns among the studied ILs in each treatment were identified.",
journal = "7th International Plant Phenotyping Symposium ‘Plant Phenotyping for a Sustainable Future’, Wageningen, the Netherlands September 26-30, 2022, IPPS 2022 Conference Book",
title = "High-throughput phenotyping of maize growth dynamics under nitrogen and water stress",
pages = "95-95",
url = "https://hdl.handle.net/21.15107/rcub_rik_1286"
}

Božinović, S., Dodig, D., Nikolić, A., Zorić, M., Vančetović, J., Ignjatović-Micić, D., Weigelt-Fischer, K., Altmann, T.,& Junker, A.. (2022). High-throughput phenotyping of maize growth dynamics under nitrogen and water stress. in 7th International Plant Phenotyping Symposium ‘Plant Phenotyping for a Sustainable Future’, Wageningen, the Netherlands September 26-30, 2022, IPPS 2022 Conference Book, 95-95.
https://hdl.handle.net/21.15107/rcub_rik_1286

Božinović S, Dodig D, Nikolić A, Zorić M, Vančetović J, Ignjatović-Micić D, Weigelt-Fischer K, Altmann T, Junker A. High-throughput phenotyping of maize growth dynamics under nitrogen and water stress. in 7th International Plant Phenotyping Symposium ‘Plant Phenotyping for a Sustainable Future’, Wageningen, the Netherlands September 26-30, 2022, IPPS 2022 Conference Book. 2022;:95-95.
https://hdl.handle.net/21.15107/rcub_rik_1286 .

Božinović, Sofija, Dodig, Dejan, Nikolić, Ana, Zorić, Miroslav, Vančetović, Jelena, Ignjatović-Micić, Dragana, Weigelt-Fischer, Kathleen, Altmann, Thomas, Junker, Astrid, "High-throughput phenotyping of maize growth dynamics under nitrogen and water stress" in 7th International Plant Phenotyping Symposium ‘Plant Phenotyping for a Sustainable Future’, Wageningen, the Netherlands September 26-30, 2022, IPPS 2022 Conference Book (2022):95-95,
https://hdl.handle.net/21.15107/rcub_rik_1286 .

TY  - CONF
AU  - Grčić, Nikola
AU  - Nikolić, Ana
AU  - Delić, Nenad
AU  - Mladenović, Marko
AU  - Čamdžija, Zoran
AU  - Božinović, Sofija
AU  - Mladenović Drinić, Snežana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1287
AB  - Maize breeding in Maize research institute “Zemun Polje” was conducted for more than 75 years resulting in the creations of numerous maize hybrids with superior traits and performance. The source breeding material used in the breeding programs was very diverse and varied from the domestic landraces to the introduced inbred lines. This resulted in a very diverse and complex structure of the breeding pools used in commercial maize breeding in “Zemun Polje” Institute. As genetic material in this study, 24 maize inbred lines of different origin, heterotic and maturity goups were selected were selected from the 3rd and 4th cycle of breeding in Maize research institute “Zemun Polje”. These are inbreds that were commonly used in breeding programs during the 1980s and were parental components of most important commercial maize hybrids. The genotyping was performed using a Maize 25k Infinium array. The neighbor-joining cladogram, PcoA and Structure were used to analyze the genetic structure and diversity of the panel. Population structure analysis of the panel initially showed a clear divide between Lancaster and Non-Lancaster inbred lines, revealing the dominant heterotic pattern in the breeding program. Further analysis with an increased number of population (k) showed a more complex structure of the non-Lancaster group of inbred lines with best number of ancestral population being k=5. The average genetic distance in the panel was 0,385, ranging from 0,039 to 0,544, with paternal inbreds in most of commercial hybrids being the most distant ones. The genotyping results of the Zemun Polje maize panel were further merged with DROPS European panel of 247 dent maize inbred lines in order to view the results of the diversity analysis of the Zemun Polje panel from a broader perspective.
PB  - Belgrade : Maize Research Institute Zemun Polje
C3  - XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts
T1  - Population structure and diversity in former ZP maize breeding program assessed with SNP markers
SP  - 32
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_rik_1287
ER  - 

@conference{
author = "Grčić, Nikola and Nikolić, Ana and Delić, Nenad and Mladenović, Marko and Čamdžija, Zoran and Božinović, Sofija and Mladenović Drinić, Snežana",
year = "2022",
abstract = "Maize breeding in Maize research institute “Zemun Polje” was conducted for more than 75 years resulting in the creations of numerous maize hybrids with superior traits and performance. The source breeding material used in the breeding programs was very diverse and varied from the domestic landraces to the introduced inbred lines. This resulted in a very diverse and complex structure of the breeding pools used in commercial maize breeding in “Zemun Polje” Institute. As genetic material in this study, 24 maize inbred lines of different origin, heterotic and maturity goups were selected were selected from the 3rd and 4th cycle of breeding in Maize research institute “Zemun Polje”. These are inbreds that were commonly used in breeding programs during the 1980s and were parental components of most important commercial maize hybrids. The genotyping was performed using a Maize 25k Infinium array. The neighbor-joining cladogram, PcoA and Structure were used to analyze the genetic structure and diversity of the panel. Population structure analysis of the panel initially showed a clear divide between Lancaster and Non-Lancaster inbred lines, revealing the dominant heterotic pattern in the breeding program. Further analysis with an increased number of population (k) showed a more complex structure of the non-Lancaster group of inbred lines with best number of ancestral population being k=5. The average genetic distance in the panel was 0,385, ranging from 0,039 to 0,544, with paternal inbreds in most of commercial hybrids being the most distant ones. The genotyping results of the Zemun Polje maize panel were further merged with DROPS European panel of 247 dent maize inbred lines in order to view the results of the diversity analysis of the Zemun Polje panel from a broader perspective.",
publisher = "Belgrade : Maize Research Institute Zemun Polje",
journal = "XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts",
title = "Population structure and diversity in former ZP maize breeding program assessed with SNP markers",
pages = "32-32",
url = "https://hdl.handle.net/21.15107/rcub_rik_1287"
}

Grčić, N., Nikolić, A., Delić, N., Mladenović, M., Čamdžija, Z., Božinović, S.,& Mladenović Drinić, S.. (2022). Population structure and diversity in former ZP maize breeding program assessed with SNP markers. in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts
Belgrade : Maize Research Institute Zemun Polje., 32-32.
https://hdl.handle.net/21.15107/rcub_rik_1287

Grčić N, Nikolić A, Delić N, Mladenović M, Čamdžija Z, Božinović S, Mladenović Drinić S. Population structure and diversity in former ZP maize breeding program assessed with SNP markers. in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts. 2022;:32-32.
https://hdl.handle.net/21.15107/rcub_rik_1287 .

Grčić, Nikola, Nikolić, Ana, Delić, Nenad, Mladenović, Marko, Čamdžija, Zoran, Božinović, Sofija, Mladenović Drinić, Snežana, "Population structure and diversity in former ZP maize breeding program assessed with SNP markers" in XXVth EUCARPIA Maize and Sorghum Conference, Current Challenges and New Methods for Maize and Sorghum Breeding, May 30 – June 2, 2022, Belgrade – Serbia, Book of Abstracts (2022):32-32,
https://hdl.handle.net/21.15107/rcub_rik_1287 .

TY  - CONF
AU  - Božić, Manja
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1152
AB  - Small non-coding RNAs (sRNAs) have an important role in regulating plant gene expression,
both in developmental processes and stress responses, through RNA interference or silencing.
sRNAs respond to environmental stressors in manners dependant on the type of stress, tissue
and genetic background. Herein, their role in chilling stress response is examined in young maize
seedlings (Zea mays L.) of two inbred lines of contrasting chilling tolerance, using high-throughput
sequencing methods. Understanding this response is critical since one of the most promising
strategies of avoiding maize yield loss due to climate change is earlier sowing, during periods
of suboptimal temperatures. Sterilized seeds of the two lines, marked as LT (tolerant), and LS
(sensitive), were germinated in the dark for five days (optimal conditions), after which the 5-d
old seedlings were exposed to chilling conditions for 24h (temperature: 10/8 °C; photoperiod:
12/12h). Samples for RNA isolation were taken after 6h and 24h of treatment. After the cDNA
library preparations were finished, single-end 50 bp sequencing was performed (Illumina® Novaseq 6000). sRNAseq analysis was performed using a custom pipeline that included quality control, mapping, known miRNA alignment and novel miRNA prediction and quantification. This is an ongoing research and a selection of results will be presented. On average 81% of sRNA reads were succesfully mapped and belong to the class of microRNAs (miRNAs). Some of the sRNAs appear to be differentialy regulated depending on the genotype and the chilling conditions, but more research is needed to verify their potential role in this process.
PB  - Belgrade : Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Chilling induced stress sRNAs in maize plants in early stages of development.
UR  - https://hdl.handle.net/21.15107/rcub_rik_1152
ER  - 

@conference{
author = "Božić, Manja and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2022",
abstract = "Small non-coding RNAs (sRNAs) have an important role in regulating plant gene expression,
both in developmental processes and stress responses, through RNA interference or silencing.
sRNAs respond to environmental stressors in manners dependant on the type of stress, tissue
and genetic background. Herein, their role in chilling stress response is examined in young maize
seedlings (Zea mays L.) of two inbred lines of contrasting chilling tolerance, using high-throughput
sequencing methods. Understanding this response is critical since one of the most promising
strategies of avoiding maize yield loss due to climate change is earlier sowing, during periods
of suboptimal temperatures. Sterilized seeds of the two lines, marked as LT (tolerant), and LS
(sensitive), were germinated in the dark for five days (optimal conditions), after which the 5-d
old seedlings were exposed to chilling conditions for 24h (temperature: 10/8 °C; photoperiod:
12/12h). Samples for RNA isolation were taken after 6h and 24h of treatment. After the cDNA
library preparations were finished, single-end 50 bp sequencing was performed (Illumina® Novaseq 6000). sRNAseq analysis was performed using a custom pipeline that included quality control, mapping, known miRNA alignment and novel miRNA prediction and quantification. This is an ongoing research and a selection of results will be presented. On average 81% of sRNA reads were succesfully mapped and belong to the class of microRNAs (miRNAs). Some of the sRNAs appear to be differentialy regulated depending on the genotype and the chilling conditions, but more research is needed to verify their potential role in this process.",
publisher = "Belgrade : Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Chilling induced stress sRNAs in maize plants in early stages of development.",
url = "https://hdl.handle.net/21.15107/rcub_rik_1152"
}

Božić, M., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2022). Chilling induced stress sRNAs in maize plants in early stages of development.. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Belgrade : Serbian Plant Physiology Society..
https://hdl.handle.net/21.15107/rcub_rik_1152

Božić M, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. Chilling induced stress sRNAs in maize plants in early stages of development.. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1152 .

Božić, Manja, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Chilling induced stress sRNAs in maize plants in early stages of development." in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022),
https://hdl.handle.net/21.15107/rcub_rik_1152 .

TY  - GEN
AU  - Božić, Manja
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1139
AB  - Circular RNAs (circRNAs) are endogenous, single-stranded, covalently closed RNA molecules, generated by back-splicing events. Their role as regulators of different processes in animal cells has been widely studied and proven, however functional studies on circRNAs in plants are still few and far between. Here we attempt to bring light to their role in abiotic stress response – particularly chilling stress, in young maize seedlings (Zea mays L.) using high-throughput sequencing methods. One of the strategies of avoiding yield loss is earlier maize sowing as to escape exposure to extremely high temperatures and drought in the summer. This means submitting maize plants to suboptimal temperatures during earlier developmental stages.
Two maize inbred lines of contrasting tolerance to chilling were selected and marked as LT (tolerant), and LS (sensitive). Sterilized seeds of the two lines were germinated for five days, in the dark, in optimal conditions and after the germination period was finished, the 5-d old seedlings were exposed to chilling conditions for 24h (temperature: 10/8° C; photoperiod: 12/12h). Sampling was done after 6h and 24h of treatment. After total RNA isolation and purification, quality check, and cDNA library preparations were finished, paired-end 150 sequencing was performed (Illumina® Novaseq 6000). Custom pipeline was used for circRNAseq data analysis and included their identification and quantification (CIRCexplorer2, v2.0.5), differential expression analysis (edgeR Bioconductor v4.1) and functional enrichment (GO, KEGG, psRobot). 
Processing of this data is still underway, and by now several thousand circRNA molecules and over a thousand 5' and 3' alternative back-splicing sites have been identified. Significant number of those circRNA have shown expression dependent on chilling, as well as genotype, but further studies are necessary to confirm their role in this process, find their target molecules and unravel regulatory networks they create with other types of RNAs.
T2  - 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts
T1  - circRNAs involved in chilling stress response during early developmental stages in maize
UR  - https://hdl.handle.net/21.15107/rcub_rik_1139
ER  - 

@misc{
author = "Božić, Manja and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2022",
abstract = "Circular RNAs (circRNAs) are endogenous, single-stranded, covalently closed RNA molecules, generated by back-splicing events. Their role as regulators of different processes in animal cells has been widely studied and proven, however functional studies on circRNAs in plants are still few and far between. Here we attempt to bring light to their role in abiotic stress response – particularly chilling stress, in young maize seedlings (Zea mays L.) using high-throughput sequencing methods. One of the strategies of avoiding yield loss is earlier maize sowing as to escape exposure to extremely high temperatures and drought in the summer. This means submitting maize plants to suboptimal temperatures during earlier developmental stages.
Two maize inbred lines of contrasting tolerance to chilling were selected and marked as LT (tolerant), and LS (sensitive). Sterilized seeds of the two lines were germinated for five days, in the dark, in optimal conditions and after the germination period was finished, the 5-d old seedlings were exposed to chilling conditions for 24h (temperature: 10/8° C; photoperiod: 12/12h). Sampling was done after 6h and 24h of treatment. After total RNA isolation and purification, quality check, and cDNA library preparations were finished, paired-end 150 sequencing was performed (Illumina® Novaseq 6000). Custom pipeline was used for circRNAseq data analysis and included their identification and quantification (CIRCexplorer2, v2.0.5), differential expression analysis (edgeR Bioconductor v4.1) and functional enrichment (GO, KEGG, psRobot). 
Processing of this data is still underway, and by now several thousand circRNA molecules and over a thousand 5' and 3' alternative back-splicing sites have been identified. Significant number of those circRNA have shown expression dependent on chilling, as well as genotype, but further studies are necessary to confirm their role in this process, find their target molecules and unravel regulatory networks they create with other types of RNAs.",
journal = "25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts",
title = "circRNAs involved in chilling stress response during early developmental stages in maize",
url = "https://hdl.handle.net/21.15107/rcub_rik_1139"
}

Božić, M., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2022). circRNAs involved in chilling stress response during early developmental stages in maize. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts.
https://hdl.handle.net/21.15107/rcub_rik_1139

Božić M, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. circRNAs involved in chilling stress response during early developmental stages in maize. in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1139 .

Božić, Manja, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "circRNAs involved in chilling stress response during early developmental stages in maize" in 25. EUCARPIA Maize and Sorghum conference current, Challenges and new methods for maize and sorghum breeding, 30.05–2.06.2022., Belgrade, Serbia - Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_rik_1139 .

TY  - PAT
AU  - Božinović, Sofija
AU  - Vančetović, Jelena
AU  - Grčić, Nikola
AU  - Nikolić, Ana
AU  - Đorđević Melnik, Olivera
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1102
T2  - Rešenje:320-44-02159/2/2020-11
T1  - ZP5023
UR  - https://hdl.handle.net/21.15107/rcub_rik_1102
ER  - 

@misc{
author = "Božinović, Sofija and Vančetović, Jelena and Grčić, Nikola and Nikolić, Ana and Đorđević Melnik, Olivera",
year = "2022",
journal = "Rešenje:320-44-02159/2/2020-11",
title = "ZP5023",
url = "https://hdl.handle.net/21.15107/rcub_rik_1102"
}

Božinović, S., Vančetović, J., Grčić, N., Nikolić, A.,& Đorđević Melnik, O.. (2022). ZP5023. in Rešenje:320-44-02159/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1102

Božinović S, Vančetović J, Grčić N, Nikolić A, Đorđević Melnik O. ZP5023. in Rešenje:320-44-02159/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1102 .

Božinović, Sofija, Vančetović, Jelena, Grčić, Nikola, Nikolić, Ana, Đorđević Melnik, Olivera, "ZP5023" in Rešenje:320-44-02159/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1102 .

TY  - CONF
AU  - Mladenović Drinić, Snežana
AU  - Srdić, Jelena
AU  - Anđelković, Violeta
AU  - Grčić, Nikola
AU  - Kravić, Natalija
AU  - Filipović, Milomir
AU  - Nikolić, Ana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/958
AB  - Popcorn is a type of specialty maize and plays important role in the history and the spread of
this species. This type of maize historicaly received little atention in genetic diversity studies
while the fact is that information on genetic variability and population structure is of crucial
importance for improvement of breeding programs. The genetic variation of popcorn maize
was mainly analysed using SSR markers, but there are not many studies applying high-density
SNP markers with lower genotyping errors. The aim of this study was the assesment of genetic
diversity and population structure of popcorn germplasm which represents the genetic
resources currently used in breeding programs in Maize Research Institute „Zemun Polje“.
Molecular characterization of 44 popcorn genotypes was done using 25k SNP Illumina Infinum
Array for maize. After quality control filtering, 12335 SNPs were selected for downstream
analyses. Genetic distance detected ranged from 0.001 to 0.51 with an average of 0.33. The
majority of genetic distances (70.6%) fell between 0.30 and 0.51 revealing significant genetic
variability in a set of maize genotypes under study. SNP markes were moderatly informative
with an average PIC value of 0.26. Also, for more than 50% of SNPs, PIC value was greater
than 0.30. The average observed heterozygosity was 0.04 ranging from 0 to 0.11. The
clustering, principal component and model-based STRUCTURE analyses showed similar
grouping of popcorn lines, generally in accordance with their genetic background. The results
of this research gave valuable information for initiation of future breeding strategies.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Molecular characterization of popcorn inbreds using 25k SNP array
UR  - https://hdl.handle.net/21.15107/rcub_rik_958
ER  - 

@conference{
author = "Mladenović Drinić, Snežana and Srdić, Jelena and Anđelković, Violeta and Grčić, Nikola and Kravić, Natalija and Filipović, Milomir and Nikolić, Ana",
year = "2022",
abstract = "Popcorn is a type of specialty maize and plays important role in the history and the spread of
this species. This type of maize historicaly received little atention in genetic diversity studies
while the fact is that information on genetic variability and population structure is of crucial
importance for improvement of breeding programs. The genetic variation of popcorn maize
was mainly analysed using SSR markers, but there are not many studies applying high-density
SNP markers with lower genotyping errors. The aim of this study was the assesment of genetic
diversity and population structure of popcorn germplasm which represents the genetic
resources currently used in breeding programs in Maize Research Institute „Zemun Polje“.
Molecular characterization of 44 popcorn genotypes was done using 25k SNP Illumina Infinum
Array for maize. After quality control filtering, 12335 SNPs were selected for downstream
analyses. Genetic distance detected ranged from 0.001 to 0.51 with an average of 0.33. The
majority of genetic distances (70.6%) fell between 0.30 and 0.51 revealing significant genetic
variability in a set of maize genotypes under study. SNP markes were moderatly informative
with an average PIC value of 0.26. Also, for more than 50% of SNPs, PIC value was greater
than 0.30. The average observed heterozygosity was 0.04 ranging from 0 to 0.11. The
clustering, principal component and model-based STRUCTURE analyses showed similar
grouping of popcorn lines, generally in accordance with their genetic background. The results
of this research gave valuable information for initiation of future breeding strategies.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Molecular characterization of popcorn inbreds using 25k SNP array",
url = "https://hdl.handle.net/21.15107/rcub_rik_958"
}

Mladenović Drinić, S., Srdić, J., Anđelković, V., Grčić, N., Kravić, N., Filipović, M.,& Nikolić, A.. (2022). Molecular characterization of popcorn inbreds using 25k SNP array. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje..
https://hdl.handle.net/21.15107/rcub_rik_958

Mladenović Drinić S, Srdić J, Anđelković V, Grčić N, Kravić N, Filipović M, Nikolić A. Molecular characterization of popcorn inbreds using 25k SNP array. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_958 .

Mladenović Drinić, Snežana, Srdić, Jelena, Anđelković, Violeta, Grčić, Nikola, Kravić, Natalija, Filipović, Milomir, Nikolić, Ana, "Molecular characterization of popcorn inbreds using 25k SNP array" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_rik_958 .

TY  - JOUR
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Marković, Ksenija
AU  - Delić, Nenad
AU  - Milivojević, Marija
AU  - Vančetović, Jelena
AU  - Mihai, Miritescu
AU  - Ignjatović-Micić, Dragana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1307
AB  - The consequences of the current climate changes sustain the strategy of early sowing (early spring) to avoid drought during the flowering
period and thus secure maize yields. Earlier sowing dates increase the risk of exposure of the plants to cold during the early development stages
and thus re-evaluation of elite material is required to select more tolerant genotypes. The first step of re-evaluation is to define characteristics that
could be used for fast survey of the elite material, before performing more profound analyses. The aim of this paper was to analyse morphological
characteristics (radicle length, coleoptile length, seedling weight) of 5 day old seedlings of two inbred lines LK and Mo17 (according to breeders
experience tolerant and susceptible, respectively) subjected to chilling stress (10°C day/8°C night) for 24h and 48h, as well as to perform
morphological (root and shoot characteristics) and physiological (chlorophyl, flavonoid and anthocyanin contents) analyses of the plants after
seven day recovery. Control plants were grown under optimal conditions (30°C day /20°C night, ≈60% humidity), at an 8h photoperiod. The
results were not straightforward, as the susceptible Mo17 showed faster recovery (statistically insignificant differences between control and
treatment plants for most of the analysed traits), while the tolerant LK was a more robust genotype throughout the experiment. Chlorophyl,
flavonoid and anthocyanin contents were not informative. The results identified survival rate and root characteristics as informative for fast
screening of seedlings for determination of cold tolerance.
PB  - Juniper publishers inc.
T2  - Agricultural research and technology
T1  - Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines
VL  - 25
IS  - 4
SP  - 5562311
DO  - 10.19080/ARTOAJ.2021.25.556311
ER  - 

@article{
author = "Božić, Manja and Nikolić, Ana and Marković, Ksenija and Delić, Nenad and Milivojević, Marija and Vančetović, Jelena and Mihai, Miritescu and Ignjatović-Micić, Dragana",
year = "2021",
abstract = "The consequences of the current climate changes sustain the strategy of early sowing (early spring) to avoid drought during the flowering
period and thus secure maize yields. Earlier sowing dates increase the risk of exposure of the plants to cold during the early development stages
and thus re-evaluation of elite material is required to select more tolerant genotypes. The first step of re-evaluation is to define characteristics that
could be used for fast survey of the elite material, before performing more profound analyses. The aim of this paper was to analyse morphological
characteristics (radicle length, coleoptile length, seedling weight) of 5 day old seedlings of two inbred lines LK and Mo17 (according to breeders
experience tolerant and susceptible, respectively) subjected to chilling stress (10°C day/8°C night) for 24h and 48h, as well as to perform
morphological (root and shoot characteristics) and physiological (chlorophyl, flavonoid and anthocyanin contents) analyses of the plants after
seven day recovery. Control plants were grown under optimal conditions (30°C day /20°C night, ≈60% humidity), at an 8h photoperiod. The
results were not straightforward, as the susceptible Mo17 showed faster recovery (statistically insignificant differences between control and
treatment plants for most of the analysed traits), while the tolerant LK was a more robust genotype throughout the experiment. Chlorophyl,
flavonoid and anthocyanin contents were not informative. The results identified survival rate and root characteristics as informative for fast
screening of seedlings for determination of cold tolerance.",
publisher = "Juniper publishers inc.",
journal = "Agricultural research and technology",
title = "Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines",
volume = "25",
number = "4",
pages = "5562311",
doi = "10.19080/ARTOAJ.2021.25.556311"
}

Božić, M., Nikolić, A., Marković, K., Delić, N., Milivojević, M., Vančetović, J., Mihai, M.,& Ignjatović-Micić, D.. (2021). Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines. in Agricultural research and technology
Juniper publishers inc.., 25(4), 5562311.
https://doi.org/10.19080/ARTOAJ.2021.25.556311

Božić M, Nikolić A, Marković K, Delić N, Milivojević M, Vančetović J, Mihai M, Ignjatović-Micić D. Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines. in Agricultural research and technology. 2021;25(4):5562311.
doi:10.19080/ARTOAJ.2021.25.556311 .

Božić, Manja, Nikolić, Ana, Marković, Ksenija, Delić, Nenad, Milivojević, Marija, Vančetović, Jelena, Mihai, Miritescu, Ignjatović-Micić, Dragana, "Surveying maize seedlings as the first step for re-evaluating cold tolerance in maize inbred lines" in Agricultural research and technology, 25, no. 4 (2021):5562311,
https://doi.org/10.19080/ARTOAJ.2021.25.556311 . .

TY  - GEN
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Djordjevic Melnik, Olivera
AU  - Pavlov, Jovan
AU  - Čamdžija, Zoran
AU  - Grčić, Nikola
AU  - Vančetović, Jelena
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1240
AB  - Doubled haploid (DH) technology is one of the several technological advances greatly impacting modern maize breeding. The main benefit for breeders is the development of parental inbred lines in one year comparing to four using conventional breeding. Maize Research Institute Zemun Polje (MRIZP) initiated doubled haploid program in maize in 2014. In six years up to 8.000 DH lines have been developed from close to 160 donor germplasm sources of different genetic backgrounds. During this period the research group engaged in the DH program identified several issues that need to be addressed in order to optimize the method for use in temperate regions including poor agronomic performance of haploid inducing lines, donor germplasm effect on the overall efficiency of DH method in maize breeding and selection strategies for improving test-cross performance of DH lines. To meet the challenges, MRIZP DH research group started a breeding program aiming at developing inducers with high and stable HIR adapted to temperate climate conditions. Two main goals were defined: development of haploid inducers with HIR up to 20% in a completely inducer genetic background, and second, to develop haploid inducers in 75 and 50% inducer genetic background with moderate HIR but better agronomic performance. Also, nine populations were used to assess the suitability of our most elite breeding materials for use as donors with respect to genetic background, specifically heterotic group origin, and give guidance to breeders on donor germplasm selection. Furthermore, we are studying different selection strategies for DH line breeding with respect to donor size and prior cycles of selection, i.e. recombination (none (F1), one (F2), or two selection cycles (F3)). We hope that our still ongoing research will contribute to already extensive knowledge on doubled haploid methodology.
PB  - Belgrade : Maize Research Institute "Zemun Polje"
T2  - International conference: The frontiers of science and technology in crop breeding and production, 8-9.06. 2021., Belgrade, Serbia - Book of abstracts
T1  - Optimization of the doubled haploid technology for temperate maize breeding programs: a case study from Maize Research Institute Zemun Polje
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rik_1240
ER  - 

@misc{
author = "Božinović, Sofija and Nikolić, Ana and Djordjevic Melnik, Olivera and Pavlov, Jovan and Čamdžija, Zoran and Grčić, Nikola and Vančetović, Jelena",
year = "2021",
abstract = "Doubled haploid (DH) technology is one of the several technological advances greatly impacting modern maize breeding. The main benefit for breeders is the development of parental inbred lines in one year comparing to four using conventional breeding. Maize Research Institute Zemun Polje (MRIZP) initiated doubled haploid program in maize in 2014. In six years up to 8.000 DH lines have been developed from close to 160 donor germplasm sources of different genetic backgrounds. During this period the research group engaged in the DH program identified several issues that need to be addressed in order to optimize the method for use in temperate regions including poor agronomic performance of haploid inducing lines, donor germplasm effect on the overall efficiency of DH method in maize breeding and selection strategies for improving test-cross performance of DH lines. To meet the challenges, MRIZP DH research group started a breeding program aiming at developing inducers with high and stable HIR adapted to temperate climate conditions. Two main goals were defined: development of haploid inducers with HIR up to 20% in a completely inducer genetic background, and second, to develop haploid inducers in 75 and 50% inducer genetic background with moderate HIR but better agronomic performance. Also, nine populations were used to assess the suitability of our most elite breeding materials for use as donors with respect to genetic background, specifically heterotic group origin, and give guidance to breeders on donor germplasm selection. Furthermore, we are studying different selection strategies for DH line breeding with respect to donor size and prior cycles of selection, i.e. recombination (none (F1), one (F2), or two selection cycles (F3)). We hope that our still ongoing research will contribute to already extensive knowledge on doubled haploid methodology.",
publisher = "Belgrade : Maize Research Institute "Zemun Polje"",
journal = "International conference: The frontiers of science and technology in crop breeding and production, 8-9.06. 2021., Belgrade, Serbia - Book of abstracts",
title = "Optimization of the doubled haploid technology for temperate maize breeding programs: a case study from Maize Research Institute Zemun Polje",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_rik_1240"
}

Božinović, S., Nikolić, A., Djordjevic Melnik, O., Pavlov, J., Čamdžija, Z., Grčić, N.,& Vančetović, J.. (2021). Optimization of the doubled haploid technology for temperate maize breeding programs: a case study from Maize Research Institute Zemun Polje. in International conference: The frontiers of science and technology in crop breeding and production, 8-9.06. 2021., Belgrade, Serbia - Book of abstracts
Belgrade : Maize Research Institute "Zemun Polje"., 44-44.
https://hdl.handle.net/21.15107/rcub_rik_1240

Božinović S, Nikolić A, Djordjevic Melnik O, Pavlov J, Čamdžija Z, Grčić N, Vančetović J. Optimization of the doubled haploid technology for temperate maize breeding programs: a case study from Maize Research Institute Zemun Polje. in International conference: The frontiers of science and technology in crop breeding and production, 8-9.06. 2021., Belgrade, Serbia - Book of abstracts. 2021;:44-44.
https://hdl.handle.net/21.15107/rcub_rik_1240 .

Božinović, Sofija, Nikolić, Ana, Djordjevic Melnik, Olivera, Pavlov, Jovan, Čamdžija, Zoran, Grčić, Nikola, Vančetović, Jelena, "Optimization of the doubled haploid technology for temperate maize breeding programs: a case study from Maize Research Institute Zemun Polje" in International conference: The frontiers of science and technology in crop breeding and production, 8-9.06. 2021., Belgrade, Serbia - Book of abstracts (2021):44-44,
https://hdl.handle.net/21.15107/rcub_rik_1240 .

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Nikolić, Ana
AU  - Jauković, Marko
AU  - Kandić, Vesna
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/836
AB  - In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Toxins
T1  - Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia
VL  - 13
SP  - 847
DO  - 10.3390/toxins13120847
ER  - 

@article{
author = "Nikolić, Milica and Savić, Iva and Nikolić, Ana and Jauković, Marko and Kandić, Vesna and Stevanović, Milan and Stanković, Slavica",
year = "2021",
abstract = "In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Toxins",
title = "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia",
volume = "13",
pages = "847",
doi = "10.3390/toxins13120847"
}

Nikolić, M., Savić, I., Nikolić, A., Jauković, M., Kandić, V., Stevanović, M.,& Stanković, S.. (2021). Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 13, 847.
https://doi.org/10.3390/toxins13120847

Nikolić M, Savić I, Nikolić A, Jauković M, Kandić V, Stevanović M, Stanković S. Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins. 2021;13:847.
doi:10.3390/toxins13120847 .

Nikolić, Milica, Savić, Iva, Nikolić, Ana, Jauković, Marko, Kandić, Vesna, Stevanović, Milan, Stanković, Slavica, "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia" in Toxins, 13 (2021):847,
https://doi.org/10.3390/toxins13120847 . .

TY  - CONF
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Dudić, Dragana
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Delić, Nenad
AU  - Banović Đeri, Bojana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1147
AB  - Finding new ways of improving crop quality, yield potential and abiotic stress tolerance are some of the most important pursuits in crop production today. As one of the biggest causes of yield and productivity reduction is climate change, specifically increasing temperatures and drought during the summer, a large number of strategies is focussed on lessening their negative effects. Cropping pattern changes include earlier sowing (early spring), when the temperatures are lower, as one of the most promising escape strategies for avoiding high summer temperatures. Thus, development of cold tolerant maize lines became an important goal.
Comparative analysis of 46 maize inbred lines belonging to two different genetic backgrounds, one predominantly cold tolerante (marked as Non-Lancaster) and the other predominantly cold sensitive (marked as Lancaster) in the field, was done by whole transriptome sequencing and differential gene expression (DGE) analysis.  Plants were grown under optimal, greenhouse conditions and sampled after completing the V4 growth stage. Total RNA isolated from leaves of three plants per inbred line was used for cDNA library preparation by Illumina TruSeq Stranded RNA LT kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent kit, v2 (2 x 150bp). Data manipulation and analysis was performed using a custom-made bioinformatics pipeline that included high throughput sequence data quality control (using FastQC), removal of low quality reads (using Trimmomatic tool, version 0.32), transcriptome assembly and mapping (using Cufflinks, version 2.2.1), expression quantification (using CuffDiff) and DGE analysis (using BLAST2GO and GO analysis Toolkit and Database for Agricultural Community, agriGO v2).  
DGE analysis revealed 77 differentially expressed genes (DEGs) between the Lancaster and the Non-Lancaster group, 21 of which were  statistically supported for differential expression between the two groups and annotated as involved in abiotic stress responses in maize and other plant species. To test DEGs response to cold stress expression of a subset of seven DEGs in eight inbred lines (4 belonging to Lancaster and 4 belonging to Non-Lancaster genetic background) was analyzed under 24h long exposure to low temperatures (6/4° C, 12h photoperiod), with sampling being done 6h and 24h after beginning of the treatment, as well as after 48h of recovery. Six DEGs showed different expression regulation dependent on cold exposure duration and genetic background.  These findings imply differently regulated processes between the analysed Lancaster and Non-Lancaster inbred lines, contributing to their different cold response and adaptation, and will be further used for the development of cold tolerant hybrids.
PB  - Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts
T1  - Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rik_1147
ER  - 

@conference{
author = "Božić, Manja and Nikolić, Ana and Dudić, Dragana and Ignjatović-Micić, Dragana and Samardžić, Jelena and Delić, Nenad and Banović Đeri, Bojana",
year = "2021",
abstract = "Finding new ways of improving crop quality, yield potential and abiotic stress tolerance are some of the most important pursuits in crop production today. As one of the biggest causes of yield and productivity reduction is climate change, specifically increasing temperatures and drought during the summer, a large number of strategies is focussed on lessening their negative effects. Cropping pattern changes include earlier sowing (early spring), when the temperatures are lower, as one of the most promising escape strategies for avoiding high summer temperatures. Thus, development of cold tolerant maize lines became an important goal.
Comparative analysis of 46 maize inbred lines belonging to two different genetic backgrounds, one predominantly cold tolerante (marked as Non-Lancaster) and the other predominantly cold sensitive (marked as Lancaster) in the field, was done by whole transriptome sequencing and differential gene expression (DGE) analysis.  Plants were grown under optimal, greenhouse conditions and sampled after completing the V4 growth stage. Total RNA isolated from leaves of three plants per inbred line was used for cDNA library preparation by Illumina TruSeq Stranded RNA LT kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent kit, v2 (2 x 150bp). Data manipulation and analysis was performed using a custom-made bioinformatics pipeline that included high throughput sequence data quality control (using FastQC), removal of low quality reads (using Trimmomatic tool, version 0.32), transcriptome assembly and mapping (using Cufflinks, version 2.2.1), expression quantification (using CuffDiff) and DGE analysis (using BLAST2GO and GO analysis Toolkit and Database for Agricultural Community, agriGO v2).  
DGE analysis revealed 77 differentially expressed genes (DEGs) between the Lancaster and the Non-Lancaster group, 21 of which were  statistically supported for differential expression between the two groups and annotated as involved in abiotic stress responses in maize and other plant species. To test DEGs response to cold stress expression of a subset of seven DEGs in eight inbred lines (4 belonging to Lancaster and 4 belonging to Non-Lancaster genetic background) was analyzed under 24h long exposure to low temperatures (6/4° C, 12h photoperiod), with sampling being done 6h and 24h after beginning of the treatment, as well as after 48h of recovery. Six DEGs showed different expression regulation dependent on cold exposure duration and genetic background.  These findings imply differently regulated processes between the analysed Lancaster and Non-Lancaster inbred lines, contributing to their different cold response and adaptation, and will be further used for the development of cold tolerant hybrids.",
publisher = "Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts",
title = "Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_rik_1147"
}

Božić, M., Nikolić, A., Dudić, D., Ignjatović-Micić, D., Samardžić, J., Delić, N.,& Banović Đeri, B.. (2021). Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature. in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts
Novi Sad : Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43.
https://hdl.handle.net/21.15107/rcub_rik_1147

Božić M, Nikolić A, Dudić D, Ignjatović-Micić D, Samardžić J, Delić N, Banović Đeri B. Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature. in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts. 2021;43.
https://hdl.handle.net/21.15107/rcub_rik_1147 .

Božić, Manja, Nikolić, Ana, Dudić, Dragana, Ignjatović-Micić, Dragana, Samardžić, Jelena, Delić, Nenad, Banović Đeri, Bojana, "Differential gene expression analysis of heterotic groups’ maize inbred lines under optimal conditions led to the identification of specific gene regulation under low-temperature" in Biologia Serbica, Belgrade bioInformatics conference 2021, 21-25.06.2021.,Vinča, Serbia. Book of abstracts, 43 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1147 .

TY  - CONF
AU  - Božić, Manja
AU  - Nikolić, Ana
AU  - Dudić, Dragana
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Banović Đeri, Bojana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1144
AB  - Improving yield potential, crop quality and abiotic stress tolerance have always been some of the most important requirements for successful crop production. Poor environmental conditions arising as repercussions of climate change, like the drastically higher temperatures and drought during the summer, have become a significant cause of productivity and yield loss. Therefore, many strategies are focused on minimizing their negative effects, like changing the cropping patterns, including earlier sowing (early spring). Maize is especially sensitive to extreme heat occurring during the flowering and grain filling stages in summer, and earlier sowing enables avoiding this. However, it also means exposure to suboptimal temperatures and chilling stress during earlier developmental stages, leading to a demand for the development of maize lines tolerant to low temperatures during those stages.
In this study, 46 maize lines used in breeding programmes were grouped as Lancaster and group consisting of different heterotic groups like BSSS, Iowa dent, etc. and further studied by whole transcriptome sequencing (maize leaves, V4 stage, optimal temperature conditions). Gene expression analyses revealed a set of 77 differentially expressed genes (DEGs) between the two groups, out of which 20 were annotated as related to abiotic stress response. ATP synthase CF0 A subunit gene (atpI) was chosen for further characterization under low temperature conditions in two inbred lines (L1, L2) with most contrasting FPKM values, one belonging to each group. The experiment was performed with 5-day old and V4 maize seedlings, under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. 
The results showed different expression regulation of atpI dependent on cold exposure duration, developmental stage and genetic background. atpI expression was up-regulated in both genotypes in V4 stage, with the expression peak after 6h of treatment. In the 5-day old seedling stage, atpI expression depended on the genotype – it was down-regulated in L1, and up-regulated in L2. The expression in both genotypes in this developmental stage was at its highest after 24h of treatment. This suggests that mechanisms involved in ATP synthesis and photosynthetic phosphorylation are differentially regulated based on low temperature exposure duration, developmental stage and genetic background.
PB  - Magnus Group (MG), USA
C3  - 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts
T1  - atpI expression in different developmental stages of maize during chilling
UR  - https://hdl.handle.net/21.15107/rcub_rik_1144
ER  - 

@conference{
author = "Božić, Manja and Nikolić, Ana and Dudić, Dragana and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Banović Đeri, Bojana",
year = "2021",
abstract = "Improving yield potential, crop quality and abiotic stress tolerance have always been some of the most important requirements for successful crop production. Poor environmental conditions arising as repercussions of climate change, like the drastically higher temperatures and drought during the summer, have become a significant cause of productivity and yield loss. Therefore, many strategies are focused on minimizing their negative effects, like changing the cropping patterns, including earlier sowing (early spring). Maize is especially sensitive to extreme heat occurring during the flowering and grain filling stages in summer, and earlier sowing enables avoiding this. However, it also means exposure to suboptimal temperatures and chilling stress during earlier developmental stages, leading to a demand for the development of maize lines tolerant to low temperatures during those stages.
In this study, 46 maize lines used in breeding programmes were grouped as Lancaster and group consisting of different heterotic groups like BSSS, Iowa dent, etc. and further studied by whole transcriptome sequencing (maize leaves, V4 stage, optimal temperature conditions). Gene expression analyses revealed a set of 77 differentially expressed genes (DEGs) between the two groups, out of which 20 were annotated as related to abiotic stress response. ATP synthase CF0 A subunit gene (atpI) was chosen for further characterization under low temperature conditions in two inbred lines (L1, L2) with most contrasting FPKM values, one belonging to each group. The experiment was performed with 5-day old and V4 maize seedlings, under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. 
The results showed different expression regulation of atpI dependent on cold exposure duration, developmental stage and genetic background. atpI expression was up-regulated in both genotypes in V4 stage, with the expression peak after 6h of treatment. In the 5-day old seedling stage, atpI expression depended on the genotype – it was down-regulated in L1, and up-regulated in L2. The expression in both genotypes in this developmental stage was at its highest after 24h of treatment. This suggests that mechanisms involved in ATP synthesis and photosynthetic phosphorylation are differentially regulated based on low temperature exposure duration, developmental stage and genetic background.",
publisher = "Magnus Group (MG), USA",
journal = "6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts",
title = "atpI expression in different developmental stages of maize during chilling",
url = "https://hdl.handle.net/21.15107/rcub_rik_1144"
}

Božić, M., Nikolić, A., Dudić, D., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Banović Đeri, B.. (2021). atpI expression in different developmental stages of maize during chilling. in 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts
Magnus Group (MG), USA..
https://hdl.handle.net/21.15107/rcub_rik_1144

Božić M, Nikolić A, Dudić D, Ignjatović-Micić D, Vančetović J, Delić N, Banović Đeri B. atpI expression in different developmental stages of maize during chilling. in 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1144 .

Božić, Manja, Nikolić, Ana, Dudić, Dragana, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Banović Đeri, Bojana, "atpI expression in different developmental stages of maize during chilling" in 6th Edition of Global Conference on Plant Science and Molecular Biology, Sept 30-Oct 01, 2021. Book of Abstracts (2021),
https://hdl.handle.net/21.15107/rcub_rik_1144 .

TY  - CONF
AU  - Božić, Manja
AU  - Banović Đeri, Bojana
AU  - Dudić, Dragana
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1146
AB  - Earlier sowing is one of the most important strategies of ensuring good yield potential and crop quality under poor environmental conditions arising as consequences of climate changes. Sowing maize in early spring, when the temperatures are lower, enables avoidance of drought and high summer temperatures during the flowering and grain filling stages, but it also means that maize plants will be exposed to suboptimal temperatures during earlier developmental stages. Consequently, development of maize lines tolerant to low temperatures during those stages becomes precedence. 
An initial study encompassed whole transcriptome sequencing of 46 maize inbred lines at V4 stage grown under optimal temperature conditions. Gene expression analysis of maize genotypes grouped as Lancaster and non-Lancaster (BSSS, Iowa dent, etc.) revealed a set of 77 differentially expressed genes (DEGs). Seven of these genes, related to abiotic stress response, were further characterized under low temperature conditions in eight inbred lines at V4 stage. Their expression showed specific profiles depending on the duration of low temperature exposure and genetic background. To test if these genes follow the same expression patterns in earlier developmental stages, the experiment was performed with 5-day old maize seedlings of two inbred lines (tolerant and sensitive), under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. Five analyzed genes showed different expression regulation dependent on cold exposure duration. Two genes showed regulation dependent both on cold exposure duration and genetic background. Additionally, three of five DEGs showed different expression patterns at 5-day old seedling stage than at V4 stage. The results imply that processes underlying maize low temperature response are differentially regulated based on stress exposure duration, developmental stage and genetic background.
PB  - Belgrade : Maize Research Institute "Zemun Polje"
C3  - International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts
T1  - Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages
UR  - https://hdl.handle.net/21.15107/rcub_rik_1146
ER  - 

@conference{
author = "Božić, Manja and Banović Đeri, Bojana and Dudić, Dragana and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2021",
abstract = "Earlier sowing is one of the most important strategies of ensuring good yield potential and crop quality under poor environmental conditions arising as consequences of climate changes. Sowing maize in early spring, when the temperatures are lower, enables avoidance of drought and high summer temperatures during the flowering and grain filling stages, but it also means that maize plants will be exposed to suboptimal temperatures during earlier developmental stages. Consequently, development of maize lines tolerant to low temperatures during those stages becomes precedence. 
An initial study encompassed whole transcriptome sequencing of 46 maize inbred lines at V4 stage grown under optimal temperature conditions. Gene expression analysis of maize genotypes grouped as Lancaster and non-Lancaster (BSSS, Iowa dent, etc.) revealed a set of 77 differentially expressed genes (DEGs). Seven of these genes, related to abiotic stress response, were further characterized under low temperature conditions in eight inbred lines at V4 stage. Their expression showed specific profiles depending on the duration of low temperature exposure and genetic background. To test if these genes follow the same expression patterns in earlier developmental stages, the experiment was performed with 5-day old maize seedlings of two inbred lines (tolerant and sensitive), under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. Five analyzed genes showed different expression regulation dependent on cold exposure duration. Two genes showed regulation dependent both on cold exposure duration and genetic background. Additionally, three of five DEGs showed different expression patterns at 5-day old seedling stage than at V4 stage. The results imply that processes underlying maize low temperature response are differentially regulated based on stress exposure duration, developmental stage and genetic background.",
publisher = "Belgrade : Maize Research Institute "Zemun Polje"",
journal = "International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts",
title = "Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages",
url = "https://hdl.handle.net/21.15107/rcub_rik_1146"
}

Božić, M., Banović Đeri, B., Dudić, D., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2021). Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages. in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts
Belgrade : Maize Research Institute "Zemun Polje"..
https://hdl.handle.net/21.15107/rcub_rik_1146

Božić M, Banović Đeri B, Dudić D, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages. in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1146 .

Božić, Manja, Banović Đeri, Bojana, Dudić, Dragana, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages" in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts (2021),
https://hdl.handle.net/21.15107/rcub_rik_1146 .

TY  - GEN
AU  - Nikolić, Ana
AU  - Banović Đeri, Bojana
AU  - Dudić, Dragana
AU  - Božić, Manja
AU  - Marković, Ksenija
AU  - Delić, Nenad
AU  - Ignjatović-Micić, Dragana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1138
AB  - Abiotic stress, as an adverse environmental condition, seriously affects and limits 
maize productivity worldwide. Climate changes lead to altering temperatures and 
rainfall patterns. Earlier maize sowing enables avoiding high temperatures during 
silking and pollination season. As a consequence of early sowing, damages 
caused by temperatures lower than optimal can occur. Hence, it is very important 
to know the mechanism of defence against the low temperatures in the early 
developmental stages of the plant. Nowadays, thanks to hightroughput methods 
such as Next Generation Sequencing (NGS), more profound and detailed research 
can be conducted. A set of maize inbred lines important for commercial breeding 
programs at Maize Research Institute, Zemun Polje was analysed by sequencing 
the whole transcriptome. The aim was to identify tolerant lines for planning future 
hybrids, try to unravel mechanism involved in maize response to low 
temperatures and to identify molecular markers that could be included in breeding 
programs. Bioinformatic analysis of Single Nucleotide Polymorphisms (SNPs) 
and Diferentially Expressed Genes (DEGs) was done. Cold-induced expression 
analysis of several DEGs revealed different expression regulation dependent on 
the duration of cold stress signalizing possible important role of these genes in 
maize response to low temperatures. A more profound transcriptome analysis, 
expected to give a comprehensive insight into low-temperature induced response 
in early stages of plant development, is already underway. Besides low temperatures, waterlogging caused by more frequent rainfalls is another abiotic 
stress which can hamper the idea of early sowing, especially if combined with 
low-temperatures. For this reason, plant response to this stress will also be studied 
on the same levels as low-temperature stress, with the final goal to develop 
hybrids which can respond to challenging climate changes.
PB  - Belgrade-Zemun : Maize Research Institute, Zemun Polje
T2  - International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts
T1  - Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_rik_1138
ER  - 

@misc{
author = "Nikolić, Ana and Banović Đeri, Bojana and Dudić, Dragana and Božić, Manja and Marković, Ksenija and Delić, Nenad and Ignjatović-Micić, Dragana",
year = "2021",
abstract = "Abiotic stress, as an adverse environmental condition, seriously affects and limits 
maize productivity worldwide. Climate changes lead to altering temperatures and 
rainfall patterns. Earlier maize sowing enables avoiding high temperatures during 
silking and pollination season. As a consequence of early sowing, damages 
caused by temperatures lower than optimal can occur. Hence, it is very important 
to know the mechanism of defence against the low temperatures in the early 
developmental stages of the plant. Nowadays, thanks to hightroughput methods 
such as Next Generation Sequencing (NGS), more profound and detailed research 
can be conducted. A set of maize inbred lines important for commercial breeding 
programs at Maize Research Institute, Zemun Polje was analysed by sequencing 
the whole transcriptome. The aim was to identify tolerant lines for planning future 
hybrids, try to unravel mechanism involved in maize response to low 
temperatures and to identify molecular markers that could be included in breeding 
programs. Bioinformatic analysis of Single Nucleotide Polymorphisms (SNPs) 
and Diferentially Expressed Genes (DEGs) was done. Cold-induced expression 
analysis of several DEGs revealed different expression regulation dependent on 
the duration of cold stress signalizing possible important role of these genes in 
maize response to low temperatures. A more profound transcriptome analysis, 
expected to give a comprehensive insight into low-temperature induced response 
in early stages of plant development, is already underway. Besides low temperatures, waterlogging caused by more frequent rainfalls is another abiotic 
stress which can hamper the idea of early sowing, especially if combined with 
low-temperatures. For this reason, plant response to this stress will also be studied 
on the same levels as low-temperature stress, with the final goal to develop 
hybrids which can respond to challenging climate changes.",
publisher = "Belgrade-Zemun : Maize Research Institute, Zemun Polje",
journal = "International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts",
title = "Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_rik_1138"
}

Nikolić, A., Banović Đeri, B., Dudić, D., Božić, M., Marković, K., Delić, N.,& Ignjatović-Micić, D.. (2021). Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.. in International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts
Belgrade-Zemun : Maize Research Institute, Zemun Polje., 50.
https://hdl.handle.net/21.15107/rcub_rik_1138

Nikolić A, Banović Đeri B, Dudić D, Božić M, Marković K, Delić N, Ignjatović-Micić D. Understanding low-temperature and waterlogging stress impact on early stages of maize plant development.. in International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts. 2021;:50.
https://hdl.handle.net/21.15107/rcub_rik_1138 .

Nikolić, Ana, Banović Đeri, Bojana, Dudić, Dragana, Božić, Manja, Marković, Ksenija, Delić, Nenad, Ignjatović-Micić, Dragana, "Understanding low-temperature and waterlogging stress impact on early stages of maize plant development." in International conference the frontiers of science and technology in crop breeding and production, June 8-9.2021., Belgrade, Serbia-Book of abstracts (2021):50,
https://hdl.handle.net/21.15107/rcub_rik_1138 .

TY  - JOUR
AU  - Banović Đeri, Bojana
AU  - Božić, Manja
AU  - Dudić, Dragana
AU  - Vićić, Ivan
AU  - Milivojević, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1137
AB  - One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes—chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.
PB  - Hoboken, New Jersey : Wiley
T2  - Journal of Agronomy and Crop Science
T1  - Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes
VL  - 208
SP  - 12529
DO  - 10.1111/jac.12529
ER  - 

@article{
author = "Banović Đeri, Bojana and Božić, Manja and Dudić, Dragana and Vićić, Ivan and Milivojević, Marija and Ignjatović-Micić, Dragana and Samardžić, Jelena and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2021",
abstract = "One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes—chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.",
publisher = "Hoboken, New Jersey : Wiley",
journal = "Journal of Agronomy and Crop Science",
title = "Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes",
volume = "208",
pages = "12529",
doi = "10.1111/jac.12529"
}

Banović Đeri, B., Božić, M., Dudić, D., Vićić, I., Milivojević, M., Ignjatović-Micić, D., Samardžić, J., Vančetović, J., Delić, N.,& Nikolić, A.. (2021). Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes. in Journal of Agronomy and Crop Science
Hoboken, New Jersey : Wiley., 208, 12529.
https://doi.org/10.1111/jac.12529

Banović Đeri B, Božić M, Dudić D, Vićić I, Milivojević M, Ignjatović-Micić D, Samardžić J, Vančetović J, Delić N, Nikolić A. Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes. in Journal of Agronomy and Crop Science. 2021;208:12529.
doi:10.1111/jac.12529 .

Banović Đeri, Bojana, Božić, Manja, Dudić, Dragana, Vićić, Ivan, Milivojević, Marija, Ignjatović-Micić, Dragana, Samardžić, Jelena, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes" in Journal of Agronomy and Crop Science, 208 (2021):12529,
https://doi.org/10.1111/jac.12529 . .

TY  - PAT
AU  - Grčić, Nikola
AU  - Filipović, Milomir
AU  - Nikolić, Ana
AU  - Mladenović, Marko
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1083
T2  - Rešenje:320-04-02261/2/2019-11
T1  - ZP6091
UR  - https://hdl.handle.net/21.15107/rcub_rik_1083
ER  - 

@misc{
author = "Grčić, Nikola and Filipović, Milomir and Nikolić, Ana and Mladenović, Marko",
year = "2021",
journal = "Rešenje:320-04-02261/2/2019-11",
title = "ZP6091",
url = "https://hdl.handle.net/21.15107/rcub_rik_1083"
}

Grčić, N., Filipović, M., Nikolić, A.,& Mladenović, M.. (2021). ZP6091. in Rešenje:320-04-02261/2/2019-11.
https://hdl.handle.net/21.15107/rcub_rik_1083

Grčić N, Filipović M, Nikolić A, Mladenović M. ZP6091. in Rešenje:320-04-02261/2/2019-11. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1083 .

Grčić, Nikola, Filipović, Milomir, Nikolić, Ana, Mladenović, Marko, "ZP6091" in Rešenje:320-04-02261/2/2019-11 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1083 .

TY  - JOUR
AU  - Božić, Manja
AU  - Stanojević, Aleksandra
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Dragana
AU  - Milivojević, Marija
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/832
AB  - Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance.
AB  - Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions
VL  - 53
IS  - 3
SP  - 1147
EP  - 1165
DO  - 10.2298/GENSR2103147B
ER  - 

@article{
author = "Božić, Manja and Stanojević, Aleksandra and Marković, Ksenija and Ignjatović-Micić, Dragana and Nikolić, Dragana and Milivojević, Marija and Nikolić, Ana",
year = "2021",
abstract = "Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance., Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions",
volume = "53",
number = "3",
pages = "1147-1165",
doi = "10.2298/GENSR2103147B"
}

Božić, M., Stanojević, A., Marković, K., Ignjatović-Micić, D., Nikolić, D., Milivojević, M.,& Nikolić, A.. (2021). Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1147-1165.
https://doi.org/10.2298/GENSR2103147B

Božić M, Stanojević A, Marković K, Ignjatović-Micić D, Nikolić D, Milivojević M, Nikolić A. Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika. 2021;53(3):1147-1165.
doi:10.2298/GENSR2103147B .

Božić, Manja, Stanojević, Aleksandra, Marković, Ksenija, Ignjatović-Micić, Dragana, Nikolić, Dragana, Milivojević, Marija, Nikolić, Ana, "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions" in Genetika, 53, no. 3 (2021):1147-1165,
https://doi.org/10.2298/GENSR2103147B . .

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Vukadinović, Jelena
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/791
AB  - Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins.
AB  - Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika
T1  - Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions
T1  - Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena
VL  - 52
IS  - 1
SP  - 273
EP  - 289
DO  - 10.2298/GENSR2001273V
ER  - 

@article{
author = "Vančetović, Jelena and Kostadinović, Marija and Božinović, Sofija and Nikolić, Ana and Vukadinović, Jelena and Marković, Ksenija and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins., Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika",
title = "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions, Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena",
volume = "52",
number = "1",
pages = "273-289",
doi = "10.2298/GENSR2001273V"
}

Vančetović, J., Kostadinović, M., Božinović, S., Nikolić, A., Vukadinović, J., Marković, K.,& Ignjatović-Micić, D.. (2020). Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika
Beograd : Društvo genetičara Srbije., 52(1), 273-289.
https://doi.org/10.2298/GENSR2001273V

Vančetović J, Kostadinović M, Božinović S, Nikolić A, Vukadinović J, Marković K, Ignjatović-Micić D. Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika. 2020;52(1):273-289.
doi:10.2298/GENSR2001273V .

Vančetović, Jelena, Kostadinović, Marija, Božinović, Sofija, Nikolić, Ana, Vukadinović, Jelena, Marković, Ksenija, Ignjatović-Micić, Dragana, "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions" in Genetika, 52, no. 1 (2020):273-289,
https://doi.org/10.2298/GENSR2001273V . .