TY  - JOUR
AU  - Nikolić, Biljana
AU  - Kovačević, Dragan
AU  - Mladenović Drinić, Snežana
AU  - Nikolić, Ana
AU  - Mitić, Zorica S.
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/719
AB  - Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites
VL  - 50
IS  - 1
SP  - 69
EP  - 84
DO  - 10.2298/GENSR1801069N
ER  - 

@article{
author = "Nikolić, Biljana and Kovačević, Dragan and Mladenović Drinić, Snežana and Nikolić, Ana and Mitić, Zorica S. and Bojović, Srđan and Marin, Petar D.",
year = "2018",
abstract = "Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites",
volume = "50",
number = "1",
pages = "69-84",
doi = "10.2298/GENSR1801069N"
}

Nikolić, B., Kovačević, D., Mladenović Drinić, S., Nikolić, A., Mitić, Z. S., Bojović, S.,& Marin, P. D.. (2018). Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites. in Genetika
Društvo genetičara Srbije, Beograd., 50(1), 69-84.
https://doi.org/10.2298/GENSR1801069N

Nikolić B, Kovačević D, Mladenović Drinić S, Nikolić A, Mitić ZS, Bojović S, Marin PD. Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites. in Genetika. 2018;50(1):69-84.
doi:10.2298/GENSR1801069N .

Nikolić, Biljana, Kovačević, Dragan, Mladenović Drinić, Snežana, Nikolić, Ana, Mitić, Zorica S., Bojović, Srđan, Marin, Petar D., "Relationships among some pines from subgenera pinus and strobus revealed by nuclear est-microsatellites" in Genetika, 50, no. 1 (2018):69-84,
https://doi.org/10.2298/GENSR1801069N . .

TY  - JOUR
AU  - Kovačević, Dragan
AU  - Nikolić, Biljana
AU  - Mladenović Drinić, Snežana
AU  - Bojović, Srđan
AU  - Dodoš, Tanja
AU  - Rajčević, Nemanja
AU  - Marin, Petar D.
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/518
AB  - Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus.
AB  - Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers
T1  - Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera
VL  - 45
IS  - 2
SP  - 493
EP  - 502
DO  - 10.2298/GENSR1302493K
ER  - 

@article{
author = "Kovačević, Dragan and Nikolić, Biljana and Mladenović Drinić, Snežana and Bojović, Srđan and Dodoš, Tanja and Rajčević, Nemanja and Marin, Petar D.",
year = "2013",
abstract = "Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus., Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers, Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera",
volume = "45",
number = "2",
pages = "493-502",
doi = "10.2298/GENSR1302493K"
}

Kovačević, D., Nikolić, B., Mladenović Drinić, S., Bojović, S., Dodoš, T., Rajčević, N.,& Marin, P. D.. (2013). Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers. in Genetika
Društvo genetičara Srbije, Beograd., 45(2), 493-502.
https://doi.org/10.2298/GENSR1302493K

Kovačević D, Nikolić B, Mladenović Drinić S, Bojović S, Dodoš T, Rajčević N, Marin PD. Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers. in Genetika. 2013;45(2):493-502.
doi:10.2298/GENSR1302493K .

Kovačević, Dragan, Nikolić, Biljana, Mladenović Drinić, Snežana, Bojović, Srđan, Dodoš, Tanja, Rajčević, Nemanja, Marin, Petar D., "Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers" in Genetika, 45, no. 2 (2013):493-502,
https://doi.org/10.2298/GENSR1302493K . .