Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers
Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera

2013
Authors
Kovačević, DraganNikolić, Biljana
Mladenović-Drinić, Snežana

Bojović, Srđan
Dodoš, Tanja
Rajčević, Nemanja
Marin, Petar D.
Article (Published version)
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Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinu...s and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus.
Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom int...rageneričkom klasifikacijom roda Pinus.
Keywords:
Serbian spruce / Bosnian pine / Macedonian pine / RAPD markersSource:
Genetika, 2013, 45, 2, 493-502Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
DOI: 10.2298/GENSR1302493K
ISSN: 0534-0012
WoS: 000328504900020
Scopus: 2-s2.0-84891503891
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MRIZPTY - JOUR AU - Kovačević, Dragan AU - Nikolić, Biljana AU - Mladenović-Drinić, Snežana AU - Bojović, Srđan AU - Dodoš, Tanja AU - Rajčević, Nemanja AU - Marin, Petar D. PY - 2013 UR - http://rik.mrizp.rs/handle/123456789/518 AB - Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus. AB - Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers T1 - Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera VL - 45 IS - 2 SP - 493 EP - 502 DO - 10.2298/GENSR1302493K UR - conv_480 ER -
@article{ author = "Kovačević, Dragan and Nikolić, Biljana and Mladenović-Drinić, Snežana and Bojović, Srđan and Dodoš, Tanja and Rajčević, Nemanja and Marin, Petar D.", year = "2013", abstract = "Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus., Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers, Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera", volume = "45", number = "2", pages = "493-502", doi = "10.2298/GENSR1302493K", url = "conv_480" }
Kovačević, D., Nikolić, B., Mladenović-Drinić, S., Bojović, S., Dodoš, T., Rajčević, N.,& Marin, P. D.. (2013). Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers. in Genetika Društvo genetičara Srbije, Beograd., 45(2), 493-502. https://doi.org/10.2298/GENSR1302493K conv_480
Kovačević D, Nikolić B, Mladenović-Drinić S, Bojović S, Dodoš T, Rajčević N, Marin PD. Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers. in Genetika. 2013;45(2):493-502. doi:10.2298/GENSR1302493K conv_480 .
Kovačević, Dragan, Nikolić, Biljana, Mladenović-Drinić, Snežana, Bojović, Srđan, Dodoš, Tanja, Rajčević, Nemanja, Marin, Petar D., "Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers" in Genetika, 45, no. 2 (2013):493-502, https://doi.org/10.2298/GENSR1302493K ., conv_480 .