TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Ignjatović-Micić, Dragana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/967
AB  - ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Nutritional and cost effects of adapted quality protein maize on broiler feeding
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rik_967
ER  - 

@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Vančetović, Jelena and Delić, Nenad and Ignjatović-Micić, Dragana",
year = "2022",
abstract = "ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Nutritional and cost effects of adapted quality protein maize on broiler feeding",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_rik_967"
}

Kostadinović, M., Ristić, D., Vančetović, J., Delić, N.,& Ignjatović-Micić, D.. (2022). Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 70.
https://hdl.handle.net/21.15107/rcub_rik_967

Kostadinović M, Ristić D, Vančetović J, Delić N, Ignjatović-Micić D. Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:70.
https://hdl.handle.net/21.15107/rcub_rik_967 .

Kostadinović, Marija, Ristić, Danijela, Vančetović, Jelena, Delić, Nenad, Ignjatović-Micić, Dragana, "Nutritional and cost effects of adapted quality protein maize on broiler feeding" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022):70,
https://hdl.handle.net/21.15107/rcub_rik_967 .

TY  - GEN
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/969
AB  - Maize has low nutritional quality due to poor content of essential amino
acids lysine and tryptophan in the dominant seed storage protein fraction,
zeins. However, Quality Protein Maize (QPM), a variety of opaque2 maize
but with good agronomic traits, can have 60% to 100% higher content of
lysine than standard maize. Monogastric animals (pigs, poultry, fish), like
humans, cannot synthesize lysine and tryptophan de novo and thus these
amino acids must be supplied through diets. QPM was primarily developed
for human consumption to overcome malnutrition in countries where maize
is staple food. Thus, QPM is of tropical origin and its adaptation to temperate
regions is frequently hampered by the retained exotic germplasm.
Development of adapted QPM hybrids is mainly aimed for feed industry. It
has been shown that substituting standard maize with QPM in feed diets
could be profitable due to improved weight gain, feed conversion ratio and
decreasing of dietary lysine supplementation. Maize Research Institute,
Zemun Polje has a program on converting elite inbred lines through marker
assisted breeding into their QPM counterparts and developing commercial
QPM hybrids. Up to now, two inbred lines have been converted, while eight
lines have passed through three or four generations of backcrossing and one
generation of selfing. Tryptophan content in the converted selfed plants was
in the range from 0.080 to 0.093. Besides developing QPM counterparts of
commercial hybrids, one QPM hybrid obtained by crossing a converted elite
inbred line and an adapted tropical inbred line was developed and tested over
different locations and in different years. This hybrid is currently used in
feeding experiments with the objective to test effects of replacing standard
maize with QPM in diets on broiler performances.
PB  - Belgrade : Maize Research Institute, Zemun Polje
T2  - International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts
T1  - Adapted quality protein maize for broiler feeds
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_rik_969
ER  - 

@misc{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad",
year = "2021",
abstract = "Maize has low nutritional quality due to poor content of essential amino
acids lysine and tryptophan in the dominant seed storage protein fraction,
zeins. However, Quality Protein Maize (QPM), a variety of opaque2 maize
but with good agronomic traits, can have 60% to 100% higher content of
lysine than standard maize. Monogastric animals (pigs, poultry, fish), like
humans, cannot synthesize lysine and tryptophan de novo and thus these
amino acids must be supplied through diets. QPM was primarily developed
for human consumption to overcome malnutrition in countries where maize
is staple food. Thus, QPM is of tropical origin and its adaptation to temperate
regions is frequently hampered by the retained exotic germplasm.
Development of adapted QPM hybrids is mainly aimed for feed industry. It
has been shown that substituting standard maize with QPM in feed diets
could be profitable due to improved weight gain, feed conversion ratio and
decreasing of dietary lysine supplementation. Maize Research Institute,
Zemun Polje has a program on converting elite inbred lines through marker
assisted breeding into their QPM counterparts and developing commercial
QPM hybrids. Up to now, two inbred lines have been converted, while eight
lines have passed through three or four generations of backcrossing and one
generation of selfing. Tryptophan content in the converted selfed plants was
in the range from 0.080 to 0.093. Besides developing QPM counterparts of
commercial hybrids, one QPM hybrid obtained by crossing a converted elite
inbred line and an adapted tropical inbred line was developed and tested over
different locations and in different years. This hybrid is currently used in
feeding experiments with the objective to test effects of replacing standard
maize with QPM in diets on broiler performances.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts",
title = "Adapted quality protein maize for broiler feeds",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_rik_969"
}

Kostadinović, M., Ignjatović-Micić, D., Vančetović, J.,& Delić, N.. (2021). Adapted quality protein maize for broiler feeds. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 75.
https://hdl.handle.net/21.15107/rcub_rik_969

Kostadinović M, Ignjatović-Micić D, Vančetović J, Delić N. Adapted quality protein maize for broiler feeds. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts. 2021;:75.
https://hdl.handle.net/21.15107/rcub_rik_969 .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, "Adapted quality protein maize for broiler feeds" in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts (2021):75,
https://hdl.handle.net/21.15107/rcub_rik_969 .

TY  - CONF
AU  - Ristić, Danijela
AU  - Perić, Vesna
AU  - Srebrić, Mirjana
AU  - Kostadinović, Marija
AU  - Kravić, Natalija
AU  - Kovinčić, Anika
AU  - Gošić-Dondo, Snežana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/966
AB  - Soybean is one of the most economically important grain crops. As the
source of plant protein and vegetable oil, it is widely used as food and
industrial crop. Quality of soybean oil is determined by fatty acid (FA)
composition. Biochemical variation of different varieties significantly
facilitates genetic screening of kernel traits and grain quality attributes, thus
leading to one of the most important breeding strategies - improvement of
the grain composition. The main objective of this work was to evaluate
whole kernel biochemical composition of eight soybean varieties and eight
lines from different breeding programs. Phytochemical characterization was
conducted for total protein and oil content, as well as for fatty acids content
(e.g. palmitic, stearic, oleic, linoleic and linolenic acid). According to
Kjedahl method, total protein content ranged from 37.01% to 39.91%. The
obtained results showed that the lowest and the highest values for oil content
were 19.00% and 21.34%, respectively. Gas Chromatography/Flame
Ionization Detector (GC-FID) was used for FA quantification and
determination. The most abundant FA was linoleic acid with a range from
47.45 to 55.7 g/100g, followed by oleic acid with detected rage from 22.59
to 29.07 g/100g of total FA content. Palmitic, linolenic and stearic FA
showed a significantly lower level varied from 9.57 to 11.51 g/100g, 5.42 to
7.18 g/100g and 3.73 to 5.86 g/100g, respectively. This variability observed
in kernel quality among soybean genotypes can provide valuable information
for future breeding.
PB  - Belgrade ;  Maize Research Institute, Zemun Polje
C3  - International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia
T1  - Biochemical characterization of soybean genotypes for grain quality improvement
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_rik_966
ER  - 

@conference{
author = "Ristić, Danijela and Perić, Vesna and Srebrić, Mirjana and Kostadinović, Marija and Kravić, Natalija and Kovinčić, Anika and Gošić-Dondo, Snežana",
year = "2021",
abstract = "Soybean is one of the most economically important grain crops. As the
source of plant protein and vegetable oil, it is widely used as food and
industrial crop. Quality of soybean oil is determined by fatty acid (FA)
composition. Biochemical variation of different varieties significantly
facilitates genetic screening of kernel traits and grain quality attributes, thus
leading to one of the most important breeding strategies - improvement of
the grain composition. The main objective of this work was to evaluate
whole kernel biochemical composition of eight soybean varieties and eight
lines from different breeding programs. Phytochemical characterization was
conducted for total protein and oil content, as well as for fatty acids content
(e.g. palmitic, stearic, oleic, linoleic and linolenic acid). According to
Kjedahl method, total protein content ranged from 37.01% to 39.91%. The
obtained results showed that the lowest and the highest values for oil content
were 19.00% and 21.34%, respectively. Gas Chromatography/Flame
Ionization Detector (GC-FID) was used for FA quantification and
determination. The most abundant FA was linoleic acid with a range from
47.45 to 55.7 g/100g, followed by oleic acid with detected rage from 22.59
to 29.07 g/100g of total FA content. Palmitic, linolenic and stearic FA
showed a significantly lower level varied from 9.57 to 11.51 g/100g, 5.42 to
7.18 g/100g and 3.73 to 5.86 g/100g, respectively. This variability observed
in kernel quality among soybean genotypes can provide valuable information
for future breeding.",
publisher = "Belgrade ;  Maize Research Institute, Zemun Polje",
journal = "International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia",
title = "Biochemical characterization of soybean genotypes for grain quality improvement",
pages = "77",
url = "https://hdl.handle.net/21.15107/rcub_rik_966"
}

Ristić, D., Perić, V., Srebrić, M., Kostadinović, M., Kravić, N., Kovinčić, A.,& Gošić-Dondo, S.. (2021). Biochemical characterization of soybean genotypes for grain quality improvement. in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia
Belgrade ;  Maize Research Institute, Zemun Polje., 77.
https://hdl.handle.net/21.15107/rcub_rik_966

Ristić D, Perić V, Srebrić M, Kostadinović M, Kravić N, Kovinčić A, Gošić-Dondo S. Biochemical characterization of soybean genotypes for grain quality improvement. in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia. 2021;:77.
https://hdl.handle.net/21.15107/rcub_rik_966 .

Ristić, Danijela, Perić, Vesna, Srebrić, Mirjana, Kostadinović, Marija, Kravić, Natalija, Kovinčić, Anika, Gošić-Dondo, Snežana, "Biochemical characterization of soybean genotypes for grain quality improvement" in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia (2021):77,
https://hdl.handle.net/21.15107/rcub_rik_966 .

TY  - CONF
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Delić, Nenad
AU  - Stanković, Goran
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/1124
AB  - In this paper agronomic characteristics of quality protein maize (QPM) hybrids adapted to temperate
regions created in Maize Research Institut Zemun Polje are presented. Hybrid ZPQPM13 showed
favorable traits for growing in these regions, and its grain yield was at the level of one of the leading
ZP hybrids - ZP 606. However, grain moisture of this hybrid was 3% higher than that of ZP 606,
pointing out to the tropical origin of its parental inbred lines. Namely, mother was obtained from F1
cross between tropical and adapted lines, and father by marker assisted selection (MAS) from QPM
line of tropical origin that was crossed and twice backcrossed with a commercial ZP line. In the future,
in order to obtain even more adapted QPM hybrids, MAS program will continue with all the best
commercial ZP inbred lines of all FAO groups.
PB  - Zagreb : University of Zagreb, Faculty of Agriculture
C3  - Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia
T1  - Agronomic traits of QPM maize hybrids adapted to temperate regions
SP  - 289
EP  - 293
UR  - https://hdl.handle.net/21.15107/rcub_rik_1124
ER  - 

@conference{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Kostadinović, Marija and Delić, Nenad and Stanković, Goran and Božinović, Sofija and Đorđević-Melnik, Olivera",
year = "2020",
abstract = "In this paper agronomic characteristics of quality protein maize (QPM) hybrids adapted to temperate
regions created in Maize Research Institut Zemun Polje are presented. Hybrid ZPQPM13 showed
favorable traits for growing in these regions, and its grain yield was at the level of one of the leading
ZP hybrids - ZP 606. However, grain moisture of this hybrid was 3% higher than that of ZP 606,
pointing out to the tropical origin of its parental inbred lines. Namely, mother was obtained from F1
cross between tropical and adapted lines, and father by marker assisted selection (MAS) from QPM
line of tropical origin that was crossed and twice backcrossed with a commercial ZP line. In the future,
in order to obtain even more adapted QPM hybrids, MAS program will continue with all the best
commercial ZP inbred lines of all FAO groups.",
publisher = "Zagreb : University of Zagreb, Faculty of Agriculture",
journal = "Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia",
title = "Agronomic traits of QPM maize hybrids adapted to temperate regions",
pages = "289-293",
url = "https://hdl.handle.net/21.15107/rcub_rik_1124"
}

Vančetović, J., Ignjatović-Micić, D., Kostadinović, M., Delić, N., Stanković, G., Božinović, S.,& Đorđević-Melnik, O.. (2020). Agronomic traits of QPM maize hybrids adapted to temperate regions. in Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia
Zagreb : University of Zagreb, Faculty of Agriculture., 289-293.
https://hdl.handle.net/21.15107/rcub_rik_1124

Vančetović J, Ignjatović-Micić D, Kostadinović M, Delić N, Stanković G, Božinović S, Đorđević-Melnik O. Agronomic traits of QPM maize hybrids adapted to temperate regions. in Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia. 2020;:289-293.
https://hdl.handle.net/21.15107/rcub_rik_1124 .

Vančetović, Jelena, Ignjatović-Micić, Dragana, Kostadinović, Marija, Delić, Nenad, Stanković, Goran, Božinović, Sofija, Đorđević-Melnik, Olivera, "Agronomic traits of QPM maize hybrids adapted to temperate regions" in Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia (2020):289-293,
https://hdl.handle.net/21.15107/rcub_rik_1124 .

TY  - CONF
AU  - Kostadinović, Marija
AU  - Janjić, Jovana
AU  - Ristić, Danijela
AU  - Đorđević-Melnik, Olivera
AU  - Božinović, Sofija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/962
AB  - This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
T1  - Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation
SP  - 37
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rik_962
ER  - 

@conference{
author = "Kostadinović, Marija and Janjić, Jovana and Ristić, Danijela and Đorđević-Melnik, Olivera and Božinović, Sofija and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2020",
abstract = "This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings",
title = "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation",
pages = "37-44",
url = "https://hdl.handle.net/21.15107/rcub_rik_962"
}

Kostadinović, M., Janjić, J., Ristić, D., Đorđević-Melnik, O., Božinović, S., Ignjatović-Micić, D.,& Vančetović, J.. (2020). Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 37-44.
https://hdl.handle.net/21.15107/rcub_rik_962

Kostadinović M, Janjić J, Ristić D, Đorđević-Melnik O, Božinović S, Ignjatović-Micić D, Vančetović J. Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings. 2020;:37-44.
https://hdl.handle.net/21.15107/rcub_rik_962 .

Kostadinović, Marija, Janjić, Jovana, Ristić, Danijela, Đorđević-Melnik, Olivera, Božinović, Sofija, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation" in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings (2020):37-44,
https://hdl.handle.net/21.15107/rcub_rik_962 .

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/970
AB  - Worldwide, modern grain breeding programs widely implement marker assisted selection (MAS). Molecular markers are being used in two ways: to control the target gene (foreground selection) and to accelerate the reconstruction of the recurrent parent genotype (background selection). Marker analysis allow breeders to discard plants without allels of interest prior to pollination, reducing the size of breeding population. Marker assisted selection for the qualitative traits, which are regulated by the action of a single or several genes and are clearly phenotypically defined, achieved the best results. The improvement of β-carotene content using crtRB1-specific molecular marker is one of the successful examples of MAS. The introgressed inbreds possessing favourable allele of crtRB1 and improved hybrids with increased β-carotene can be used in the biofortification program.
The results presented herein are a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions. The utility of SSR marker specific to the crtRB1 gene in foreground selection has been already tested on parental lines and distinct marker polymorphism was observed between the donor and recurrent parents. The objective of this study was to validate the ability of the same SSR marker to detect homozygous dominant and heterozygous plants separately in BC1 generation. Heterozygous individuals were clearly distinguishable, therefore they will be selected and backcrossed with the recurrent parent to produce BC2 generation. Percentage of heterozygous plants identified in BC1 generation was approximately 50%, which is in accordance with the expected Mendelian ratio of 1 O2O2 : 1 O2o2. The use of the crtRB1-specific marker will be extended in the next generation of MAS for β-carotene enriched lines for growing in temperate regions.
PB  - London : Insight Medical Publishing
C3  - 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research
T1  - Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation
VL  - 09
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_rik_970
ER  - 

@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Mladenović Drinić, Snežana",
year = "2019",
abstract = "Worldwide, modern grain breeding programs widely implement marker assisted selection (MAS). Molecular markers are being used in two ways: to control the target gene (foreground selection) and to accelerate the reconstruction of the recurrent parent genotype (background selection). Marker analysis allow breeders to discard plants without allels of interest prior to pollination, reducing the size of breeding population. Marker assisted selection for the qualitative traits, which are regulated by the action of a single or several genes and are clearly phenotypically defined, achieved the best results. The improvement of β-carotene content using crtRB1-specific molecular marker is one of the successful examples of MAS. The introgressed inbreds possessing favourable allele of crtRB1 and improved hybrids with increased β-carotene can be used in the biofortification program.
The results presented herein are a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions. The utility of SSR marker specific to the crtRB1 gene in foreground selection has been already tested on parental lines and distinct marker polymorphism was observed between the donor and recurrent parents. The objective of this study was to validate the ability of the same SSR marker to detect homozygous dominant and heterozygous plants separately in BC1 generation. Heterozygous individuals were clearly distinguishable, therefore they will be selected and backcrossed with the recurrent parent to produce BC2 generation. Percentage of heterozygous plants identified in BC1 generation was approximately 50%, which is in accordance with the expected Mendelian ratio of 1 O2O2 : 1 O2o2. The use of the crtRB1-specific marker will be extended in the next generation of MAS for β-carotene enriched lines for growing in temperate regions.",
publisher = "London : Insight Medical Publishing",
journal = "5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research",
title = "Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation",
volume = "09",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_rik_970"
}

Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D.,& Mladenović Drinić, S.. (2019). Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation. in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research
London : Insight Medical Publishing., 09, 27.
https://hdl.handle.net/21.15107/rcub_rik_970

Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Mladenović Drinić S. Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation. in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research. 2019;09:27.
https://hdl.handle.net/21.15107/rcub_rik_970 .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Mladenović Drinić, Snežana, "Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation" in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research, 09 (2019):27,
https://hdl.handle.net/21.15107/rcub_rik_970 .

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/743
AB  - Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije.
AB  - The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Marker assisted backcross breeding in Maize Research Institute Zemun Polje
T1  - Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje
VL  - 25
IS  - 1
SP  - 41
EP  - 47
DO  - 10.5937/SelSem1901041K
ER  - 

@article{
author = "Kostadinović, Marija and Nikolić, Ana and Ristić, Danijela and Božinović, Sofija and Đorđević-Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije., The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Marker assisted backcross breeding in Maize Research Institute Zemun Polje, Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje",
volume = "25",
number = "1",
pages = "41-47",
doi = "10.5937/SelSem1901041K"
}

Kostadinović, M., Nikolić, A., Ristić, D., Božinović, S., Đorđević-Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2019). Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 41-47.
https://doi.org/10.5937/SelSem1901041K

Kostadinović M, Nikolić A, Ristić D, Božinović S, Đorđević-Melnik O, Ignjatović-Micić D, Vančetović J. Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo. 2019;25(1):41-47.
doi:10.5937/SelSem1901041K .

Kostadinović, Marija, Nikolić, Ana, Ristić, Danijela, Božinović, Sofija, Đorđević-Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted backcross breeding in Maize Research Institute Zemun Polje" in Selekcija i semenarstvo, 25, no. 1 (2019):41-47,
https://doi.org/10.5937/SelSem1901041K . .

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Kovinčić, Anika
AU  - Simić, Marijana
AU  - Perić, Vesna
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/963
AB  - Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.
PB  - Belgrade : Serbian Genetic Society
C3  - 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
T1  - The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version
SP  - 23
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_rik_963
ER  - 

@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Kovinčić, Anika and Simić, Marijana and Perić, Vesna and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings",
title = "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version",
pages = "23-30",
url = "https://hdl.handle.net/21.15107/rcub_rik_963"
}

Kostadinović, M., Ristić, D., Kovinčić, A., Simić, M., Perić, V., Ignjatović-Micić, D.,& Vančetović, J.. (2019). The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
Belgrade : Serbian Genetic Society., 23-30.
https://hdl.handle.net/21.15107/rcub_rik_963

Kostadinović M, Ristić D, Kovinčić A, Simić M, Perić V, Ignjatović-Micić D, Vančetović J. The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings. 2019;:23-30.
https://hdl.handle.net/21.15107/rcub_rik_963 .

Kostadinović, Marija, Ristić, Danijela, Kovinčić, Anika, Simić, Marijana, Perić, Vesna, Ignjatović-Micić, Dragana, Vančetović, Jelena, "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version" in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings (2019):23-30,
https://hdl.handle.net/21.15107/rcub_rik_963 .

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Đorđević-Melnik, Olivera
AU  - Kovinčić, Anika
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/964
AB  - Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
T1  - Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines
SP  - 91
EP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rik_964
ER  - 

@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Đorđević-Melnik, Olivera and Kovinčić, Anika",
year = "2019",
abstract = "Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings",
title = "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines",
pages = "91-95",
url = "https://hdl.handle.net/21.15107/rcub_rik_964"
}

Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Đorđević-Melnik, O.,& Kovinčić, A.. (2019). Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 91-95.
https://hdl.handle.net/21.15107/rcub_rik_964

Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Đorđević-Melnik O, Kovinčić A. Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings. 2019;:91-95.
https://hdl.handle.net/21.15107/rcub_rik_964 .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Đorđević-Melnik, Olivera, Kovinčić, Anika, "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines" in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings (2019):91-95,
https://hdl.handle.net/21.15107/rcub_rik_964 .

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Stevanović, Milan
AU  - Mladenović Drinić, Snežana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/960
AB  - Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
T1  - Parental polymorphism analysis in marker assisted selection for β-carotene rich maize
SP  - 333
EP  - 338
UR  - https://hdl.handle.net/21.15107/rcub_rik_960
ER  - 

@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Stevanović, Milan and Mladenović Drinić, Snežana",
year = "2018",
abstract = "Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings",
title = "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize",
pages = "333-338",
url = "https://hdl.handle.net/21.15107/rcub_rik_960"
}

Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Stevanović, M.,& Mladenović Drinić, S.. (2018). Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 333-338.
https://hdl.handle.net/21.15107/rcub_rik_960

Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Stevanović M, Mladenović Drinić S. Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings. 2018;:333-338.
https://hdl.handle.net/21.15107/rcub_rik_960 .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Stevanović, Milan, Mladenović Drinić, Snežana, "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize" in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings (2018):333-338,
https://hdl.handle.net/21.15107/rcub_rik_960 .

TY  - GEN
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Delić, Nenad
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/961
AB  - Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića.
AB  - Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
T2  - 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
T1  - Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna
T1  - Marker assisted selection for improving protein quality of maize grain
SP  - 170
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_rik_961
ER  - 

@misc{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Stanković, Goran and Delić, Nenad",
year = "2018",
abstract = "Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića., Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata",
title = "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna, Marker assisted selection for improving protein quality of maize grain",
pages = "170-171",
url = "https://hdl.handle.net/21.15107/rcub_rik_961"
}

Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Stanković, G.,& Delić, N.. (2018). Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
Beograd : Društvo genetičara Srbije., 170-171.
https://hdl.handle.net/21.15107/rcub_rik_961

Kostadinović M, Ignjatović-Micić D, Vančetović J, Stanković G, Delić N. Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata. 2018;:170-171.
https://hdl.handle.net/21.15107/rcub_rik_961 .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Stanković, Goran, Delić, Nenad, "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna" in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata (2018):170-171,
https://hdl.handle.net/21.15107/rcub_rik_961 .

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Trbović, Dejana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/684
AB  - A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.
PB  - Univ Sao Paolo, Cerquera Cesar
T2  - Scientia Agricola
T1  - Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends
VL  - 74
IS  - 6
SP  - 461
EP  - 468
DO  - 10.1590/1678-992X-2016-0302
ER  - 

@article{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Božinović, Sofija and Nikolić, Ana and Kostadinović, Marija and Trbović, Dejana",
year = "2017",
abstract = "A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.",
publisher = "Univ Sao Paolo, Cerquera Cesar",
journal = "Scientia Agricola",
title = "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends",
volume = "74",
number = "6",
pages = "461-468",
doi = "10.1590/1678-992X-2016-0302"
}

Vančetović, J., Ignjatović-Micić, D., Delić, N., Božinović, S., Nikolić, A., Kostadinović, M.,& Trbović, D.. (2017). Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola
Univ Sao Paolo, Cerquera Cesar., 74(6), 461-468.
https://doi.org/10.1590/1678-992X-2016-0302

Vančetović J, Ignjatović-Micić D, Delić N, Božinović S, Nikolić A, Kostadinović M, Trbović D. Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola. 2017;74(6):461-468.
doi:10.1590/1678-992X-2016-0302 .

Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Božinović, Sofija, Nikolić, Ana, Kostadinović, Marija, Trbović, Dejana, "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends" in Scientia Agricola, 74, no. 6 (2017):461-468,
https://doi.org/10.1590/1678-992X-2016-0302 . .

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Ignjatović-Micić, Dragana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/679
AB  - Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index.
AB  - Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Comparison of statistical methods for genetic similarity evaluation of maize inbred lines
T1  - Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza
VL  - 54
IS  - 1
SP  - 25
EP  - 30
DO  - 10.5937/ratpov54-12174
ER  - 

@article{
author = "Nikolić, Ana and Kostadinović, Marija and Vančetović, Jelena and Stanković, Goran and Ignjatović-Micić, Dragana",
year = "2017",
abstract = "Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index., Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines, Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza",
volume = "54",
number = "1",
pages = "25-30",
doi = "10.5937/ratpov54-12174"
}

Nikolić, A., Kostadinović, M., Vančetović, J., Stanković, G.,& Ignjatović-Micić, D.. (2017). Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(1), 25-30.
https://doi.org/10.5937/ratpov54-12174

Nikolić A, Kostadinović M, Vančetović J, Stanković G, Ignjatović-Micić D. Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo. 2017;54(1):25-30.
doi:10.5937/ratpov54-12174 .

Nikolić, Ana, Kostadinović, Marija, Vančetović, Jelena, Stanković, Goran, Ignjatović-Micić, Dragana, "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines" in Ratarstvo i povrtarstvo, 54, no. 1 (2017):25-30,
https://doi.org/10.5937/ratpov54-12174 . .

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Nikolić, Aleksandra
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/680
AB  - Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - DNA and biochemical analysis of a potential opaque2 maize population
VL  - 49
IS  - 1
SP  - 51
EP  - 61
DO  - 10.2298/GENSR1701051I
ER  - 

@article{
author = "Ignjatović-Micić, Dragana and Nikolić, Ana and Kostadinović, Marija and Nikolić, Aleksandra",
year = "2017",
abstract = "Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "DNA and biochemical analysis of a potential opaque2 maize population",
volume = "49",
number = "1",
pages = "51-61",
doi = "10.2298/GENSR1701051I"
}

Ignjatović-Micić, D., Nikolić, A., Kostadinović, M.,& Nikolić, A.. (2017). DNA and biochemical analysis of a potential opaque2 maize population. in Genetika
Društvo genetičara Srbije, Beograd., 49(1), 51-61.
https://doi.org/10.2298/GENSR1701051I

Ignjatović-Micić D, Nikolić A, Kostadinović M, Nikolić A. DNA and biochemical analysis of a potential opaque2 maize population. in Genetika. 2017;49(1):51-61.
doi:10.2298/GENSR1701051I .

Ignjatović-Micić, Dragana, Nikolić, Ana, Kostadinović, Marija, Nikolić, Aleksandra, "DNA and biochemical analysis of a potential opaque2 maize population" in Genetika, 49, no. 1 (2017):51-61,
https://doi.org/10.2298/GENSR1701051I . .

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Stanković, Goran
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/621
AB  - Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.
PB  - Public Library Science, San Francisco
T2  - PLOS ONE
T1  - Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167635
ER  - 

@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Ristić, Danijela and Mladenović Drinić, Snežana and Vančetović, Jelena and Božinović, Sofija and Stanković, Goran",
year = "2016",
abstract = "Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.",
publisher = "Public Library Science, San Francisco",
journal = "PLOS ONE",
title = "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167635"
}

Kostadinović, M., Ignjatović-Micić, D., Ristić, D., Mladenović Drinić, S., Vančetović, J., Božinović, S.,& Stanković, G.. (2016). Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167635

Kostadinović M, Ignjatović-Micić D, Ristić D, Mladenović Drinić S, Vančetović J, Božinović S, Stanković G. Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE. 2016;11(12).
doi:10.1371/journal.pone.0167635 .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Ristić, Danijela, Mladenović Drinić, Snežana, Vančetović, Jelena, Božinović, Sofija, Stanković, Goran, "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits" in PLOS ONE, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167635 . .

TY  - THES
AU  - Kostadinović, Marija
PY  - 2015
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=4404
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:14312/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025026994
UR  - http://nardus.mpn.gov.rs/123456789/7283
UR  - http://rik.mrizp.rs/handle/123456789/753
AB  - Hranljiva vrednost kukuruza je niska zbog niskog sadržaja dve esencijalne aminokiseline - lizina i triptofana. Poboljšanje hranljive vrednosti kukuruza predstavlja jedan od glavnih ciljeva savremenih programa oplemenjivanja zbog sve značajnijih klimatskih promena i rasta ljudske populacije, odnosno sve veće potrebe za hranom. Najveći uspeh postignut je 60-ih godina prošlog veka otkrićem opaque2 (o2) mutanta sa superiornim hranljivim svojstvima. Međutim, plejotropni efekti o2 mutacije čine endosperm zrna kukuruza mekim, a samo zrno osetljivim na pucanje, trulež i štetočine. Istraživači Međunarodnog centra za poboljšanje pšenice i kukuruza u Meksiku (eng. International Maize and Wheat Improvement Center - CIMMYT) su metodama konvencionalnog oplemenjivanja stvorili kukuruz visokog kvaliteta proteina (eng. Quality Protein Maize – QPM), koji istovremeno ima visoku hranljivu vrednost proteina endosperma i dobre agronomske performanse.Iako je QPM razvijen klasičnim metodama selekcije, poslednjih godina se sve više primenjuje selekcija upotrebom molekularnih markera (eng. Marker Assisted Selection - MAS) sa ciljem poboljšanja elitnih linija za kvalitet proteina. Unutar sekvence o2 gena identifikovana su tri različita SSR markera – phi057, phi112 i umc1066, koji se koriste za utvrđivanje polimorfizma inbred linija i kao selekcioni markeri za o2 gen (eng. foreground selection). Molekularni markeri se koriste i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (eng. background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.Osnovni cilj ovog rada bio je dobijanje linija kukuruza poboljšanog kvaliteta proteina adaptiranih na umereno klimatsko područje, korišćenjem specifičnih molekularnih markera za opaque2 gen, kao i da se održe dobre agronomske osobine i kombinaciona sposobnost poboljšanih linija...
AB  - Maize nutritional quality is poor due to the low levels of two essential aminoacids - lysine and tryptophan. Because of the climatic changes and increased food demands due to the human population growth, improvement of the maize nutritional quality is one of the main goals of many breeding programs. A major breakthrough has been the isolation of the opaque2 (o2) mutant with superior nutritional properties in the 1960s. However, pleiotropic effect of the o2 mutation makes the maize endosperm soft and susceptible to cracking, ear rots, and storage pests. Using conventional breeding methodologies, interdisciplinary research team in the International Maize and Wheat Improvement Center (CIMMYT), Mexico, created the new, agronomically acceptable and nutritionally improved opaque2 types by the name of Quality Protein Maize (QPM).Although QPM was created through conventional breeding, marker assisted selection (MAS) is becoming increasingly used approach for improvement of protein quality in maize. Three simple sequence repeats (phi057, phi112 and umc1066), located as internal repetitive sequences within the o2 gene, are being utilized as foreground selection markers for the o2 gene. Molecular markers are also effectively employed for identification of the genotypes with the highest proportion of recurrent parent genome (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection.The main objective of this research was to create high quality protein maize lines adapted to temperate regions using opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Two elite inbred lines - ZPL 3 and ZPL 5 were selected as the recurrent parents, to be the recipients of the o2 allele and to improve the tryptophan content. CML 144, a QPM tropical inbred line, was selected as the donor line of o2. Identification and confirmation of the QPM selection results involved biochemical analysis (determination of proteincontent, tryptophan content and quality index - tryptophan to protein ratio) and evaluation of the agronomicaly important traits in the field trials...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Selekcija linija kukuruza sa poboljšanim kvalitetom proteina i adaptiranih na umereno klimatsko područje upotrebom molekularnih markera
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7283
ER  - 

@phdthesis{
author = "Kostadinović, Marija",
year = "2015",
abstract = "Hranljiva vrednost kukuruza je niska zbog niskog sadržaja dve esencijalne aminokiseline - lizina i triptofana. Poboljšanje hranljive vrednosti kukuruza predstavlja jedan od glavnih ciljeva savremenih programa oplemenjivanja zbog sve značajnijih klimatskih promena i rasta ljudske populacije, odnosno sve veće potrebe za hranom. Najveći uspeh postignut je 60-ih godina prošlog veka otkrićem opaque2 (o2) mutanta sa superiornim hranljivim svojstvima. Međutim, plejotropni efekti o2 mutacije čine endosperm zrna kukuruza mekim, a samo zrno osetljivim na pucanje, trulež i štetočine. Istraživači Međunarodnog centra za poboljšanje pšenice i kukuruza u Meksiku (eng. International Maize and Wheat Improvement Center - CIMMYT) su metodama konvencionalnog oplemenjivanja stvorili kukuruz visokog kvaliteta proteina (eng. Quality Protein Maize – QPM), koji istovremeno ima visoku hranljivu vrednost proteina endosperma i dobre agronomske performanse.Iako je QPM razvijen klasičnim metodama selekcije, poslednjih godina se sve više primenjuje selekcija upotrebom molekularnih markera (eng. Marker Assisted Selection - MAS) sa ciljem poboljšanja elitnih linija za kvalitet proteina. Unutar sekvence o2 gena identifikovana su tri različita SSR markera – phi057, phi112 i umc1066, koji se koriste za utvrđivanje polimorfizma inbred linija i kao selekcioni markeri za o2 gen (eng. foreground selection). Molekularni markeri se koriste i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (eng. background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.Osnovni cilj ovog rada bio je dobijanje linija kukuruza poboljšanog kvaliteta proteina adaptiranih na umereno klimatsko područje, korišćenjem specifičnih molekularnih markera za opaque2 gen, kao i da se održe dobre agronomske osobine i kombinaciona sposobnost poboljšanih linija..., Maize nutritional quality is poor due to the low levels of two essential aminoacids - lysine and tryptophan. Because of the climatic changes and increased food demands due to the human population growth, improvement of the maize nutritional quality is one of the main goals of many breeding programs. A major breakthrough has been the isolation of the opaque2 (o2) mutant with superior nutritional properties in the 1960s. However, pleiotropic effect of the o2 mutation makes the maize endosperm soft and susceptible to cracking, ear rots, and storage pests. Using conventional breeding methodologies, interdisciplinary research team in the International Maize and Wheat Improvement Center (CIMMYT), Mexico, created the new, agronomically acceptable and nutritionally improved opaque2 types by the name of Quality Protein Maize (QPM).Although QPM was created through conventional breeding, marker assisted selection (MAS) is becoming increasingly used approach for improvement of protein quality in maize. Three simple sequence repeats (phi057, phi112 and umc1066), located as internal repetitive sequences within the o2 gene, are being utilized as foreground selection markers for the o2 gene. Molecular markers are also effectively employed for identification of the genotypes with the highest proportion of recurrent parent genome (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection.The main objective of this research was to create high quality protein maize lines adapted to temperate regions using opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Two elite inbred lines - ZPL 3 and ZPL 5 were selected as the recurrent parents, to be the recipients of the o2 allele and to improve the tryptophan content. CML 144, a QPM tropical inbred line, was selected as the donor line of o2. Identification and confirmation of the QPM selection results involved biochemical analysis (determination of proteincontent, tryptophan content and quality index - tryptophan to protein ratio) and evaluation of the agronomicaly important traits in the field trials...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Selekcija linija kukuruza sa poboljšanim kvalitetom proteina i adaptiranih na umereno klimatsko područje upotrebom molekularnih markera",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7283"
}

Kostadinović, M.. (2015). Selekcija linija kukuruza sa poboljšanim kvalitetom proteina i adaptiranih na umereno klimatsko područje upotrebom molekularnih markera. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_7283

Kostadinović M. Selekcija linija kukuruza sa poboljšanim kvalitetom proteina i adaptiranih na umereno klimatsko područje upotrebom molekularnih markera. in Универзитет у Београду. 2015;.
https://hdl.handle.net/21.15107/rcub_nardus_7283 .

Kostadinović, Marija, "Selekcija linija kukuruza sa poboljšanim kvalitetom proteina i adaptiranih na umereno klimatsko područje upotrebom molekularnih markera" in Универзитет у Београду (2015),
https://hdl.handle.net/21.15107/rcub_nardus_7283 .

TY  - JOUR
AU  - Božinović, Sofija
AU  - Vančetović, Jelena
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Prodanović, Slaven
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/588
AB  - Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield
VL  - 75
IS  - 2
SP  - 160
EP  - 167
DO  - 10.4067/S0718-58392015000200004
ER  - 

@article{
author = "Božinović, Sofija and Vančetović, Jelena and Nikolić, Ana and Ristić, Danijela and Kostadinović, Marija and Ignjatović-Micić, Dragana and Prodanović, Slaven",
year = "2015",
abstract = "Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield",
volume = "75",
number = "2",
pages = "160-167",
doi = "10.4067/S0718-58392015000200004"
}

Božinović, S., Vančetović, J., Nikolić, A., Ristić, D., Kostadinović, M., Ignjatović-Micić, D.,& Prodanović, S.. (2015). Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 75(2), 160-167.
https://doi.org/10.4067/S0718-58392015000200004

Božinović S, Vančetović J, Nikolić A, Ristić D, Kostadinović M, Ignjatović-Micić D, Prodanović S. Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research. 2015;75(2):160-167.
doi:10.4067/S0718-58392015000200004 .

Božinović, Sofija, Vančetović, Jelena, Nikolić, Ana, Ristić, Danijela, Kostadinović, Marija, Ignjatović-Micić, Dragana, Prodanović, Slaven, "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield" in Chilean Journal of Agricultural Research, 75, no. 2 (2015):160-167,
https://doi.org/10.4067/S0718-58392015000200004 . .

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Dumanović, Zoran
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Trbović, Dejana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/573
AB  - A total of 13 maize populations from the drought-tolerant mini core collection from Maize Research Institute gene bank were evaluated for oil, protein, and tryptophan contents, fatty acid (FA) composition, and kernel characteristics. All accessions are high oil (5.8-7.9%) and protein (10.58-12.45%) genotypes. Most of the accessions showed high contents of tryptophan (0.070-0.081%) and saturated (12.65-17.91%) and monounsaturated (24.19-45.52%) FAs. Significant positive correlations were found between oil and protein and between oil and tryptophan contents (p  lt  0.01). Correlations between oil and principal FA were non-significant. Several accessions showed multiple nutritional advantages. For example, IP6428 had high oil (7.3%), tryptophan (0.081%), and saturated FA (17.9%) contents. Moreover, a positive correlation (p  lt  0.01) between palmitic (13.68%) and oleic (34.74%) acids enables the use of IP6428 for developing lines high in these FAs. Because drought-tolerant accessions were selected in both subtropical and temperate zones, they could be used for breeding value-added maize adapted to both environments.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Grain Nutrient Composition of Maize (Zea mays L.) Drought-Tolerant Populations
VL  - 63
IS  - 4
SP  - 1251
EP  - 1260
DO  - 10.1021/jf504301u
ER  - 

@article{
author = "Ignjatović-Micić, Dragana and Vančetović, Jelena and Dumanović, Zoran and Kostadinović, Marija and Božinović, Sofija and Trbović, Dejana",
year = "2015",
abstract = "A total of 13 maize populations from the drought-tolerant mini core collection from Maize Research Institute gene bank were evaluated for oil, protein, and tryptophan contents, fatty acid (FA) composition, and kernel characteristics. All accessions are high oil (5.8-7.9%) and protein (10.58-12.45%) genotypes. Most of the accessions showed high contents of tryptophan (0.070-0.081%) and saturated (12.65-17.91%) and monounsaturated (24.19-45.52%) FAs. Significant positive correlations were found between oil and protein and between oil and tryptophan contents (p  lt  0.01). Correlations between oil and principal FA were non-significant. Several accessions showed multiple nutritional advantages. For example, IP6428 had high oil (7.3%), tryptophan (0.081%), and saturated FA (17.9%) contents. Moreover, a positive correlation (p  lt  0.01) between palmitic (13.68%) and oleic (34.74%) acids enables the use of IP6428 for developing lines high in these FAs. Because drought-tolerant accessions were selected in both subtropical and temperate zones, they could be used for breeding value-added maize adapted to both environments.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Grain Nutrient Composition of Maize (Zea mays L.) Drought-Tolerant Populations",
volume = "63",
number = "4",
pages = "1251-1260",
doi = "10.1021/jf504301u"
}

Ignjatović-Micić, D., Vančetović, J., Dumanović, Z., Kostadinović, M., Božinović, S.,& Trbović, D.. (2015). Grain Nutrient Composition of Maize (Zea mays L.) Drought-Tolerant Populations. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 63(4), 1251-1260.
https://doi.org/10.1021/jf504301u

Ignjatović-Micić D, Vančetović J, Dumanović Z, Kostadinović M, Božinović S, Trbović D. Grain Nutrient Composition of Maize (Zea mays L.) Drought-Tolerant Populations. in Journal of Agricultural and Food Chemistry. 2015;63(4):1251-1260.
doi:10.1021/jf504301u .

Ignjatović-Micić, Dragana, Vančetović, Jelena, Dumanović, Zoran, Kostadinović, Marija, Božinović, Sofija, Trbović, Dejana, "Grain Nutrient Composition of Maize (Zea mays L.) Drought-Tolerant Populations" in Journal of Agricultural and Food Chemistry, 63, no. 4 (2015):1251-1260,
https://doi.org/10.1021/jf504301u . .

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/526
AB  - Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize
VL  - 46
IS  - 2
SP  - 579
EP  - 590
DO  - 10.2298/GENSR1402579K
ER  - 

@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović Drinić, Snežana",
year = "2014",
abstract = "Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize",
volume = "46",
number = "2",
pages = "579-590",
doi = "10.2298/GENSR1402579K"
}

Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović Drinić, S.. (2014). Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika
Društvo genetičara Srbije, Beograd., 46(2), 579-590.
https://doi.org/10.2298/GENSR1402579K

Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović Drinić S. Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika. 2014;46(2):579-590.
doi:10.2298/GENSR1402579K .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović Drinić, Snežana, "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize" in Genetika, 46, no. 2 (2014):579-590,
https://doi.org/10.2298/GENSR1402579K . .

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Anđelković, Violeta
AU  - Vančetović, Jelena
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/525
AB  - Maize (Zea mays L.) landraces are an important source of genes for improving commercial germplasm. Today, drought tolerance and grain quality are major challenges in maize cultivation due to climatic changes and population growth. The Maize Research Institute gene-bank has a drought tolerant collection, which includes 13 landraces (from the former Yugoslavia) and 12 introduced populations (from different countries). These accessions were analyzed for protein, oil, starch and tryptophan contents, in order to identify drought tolerant accessions with high grain quality. Also, simple sequence repeat (SSR) analysis with specific primers for opaque2 recessive allele (o2) was carried out. All analyzed accessions showed high levels of protein. Oil content ranged from 3.75 % to 5.40 % and starch content from 67.5 % to 71.30%. Average protein content was not different (p  lt  0.01) between landraces and introduced populations. Starch and oil contents were higher in introduced populations at 0.84 % and 0.39 %, respectively (p  lt  0.01). Twenty-three accessions had high levels of tryptophan content. A high percentage of kernel type 1 and 2 indicated the presence of endosperm hardness modifier genes. Recessive o2 allele was found in most of the accessions. Absence of o2 in some high tryptophan accessions indicated action of another mutation. In two high tryptophan accessions an unknown band was detected. Absence of negative correlations between proteins, tryptophan and oil makes certain accessions suitable for use in the simultaneous improvement of target genotypes for these traits. Identified drought tolerant, high quality accessions can be used in breeding programs aimed at nutritional improvement of maize grown under drought conditions.
PB  - Univ Sao Paolo, Cerquera Cesar
T2  - Scientia Agricola
T1  - High grain quality accessions within a maize drought tolerant core collection
VL  - 71
IS  - 5
SP  - 402
EP  - 409
DO  - 10.1590/0103-9016-2013-0112
ER  - 

@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Božinović, Sofija and Anđelković, Violeta and Vančetović, Jelena",
year = "2014",
abstract = "Maize (Zea mays L.) landraces are an important source of genes for improving commercial germplasm. Today, drought tolerance and grain quality are major challenges in maize cultivation due to climatic changes and population growth. The Maize Research Institute gene-bank has a drought tolerant collection, which includes 13 landraces (from the former Yugoslavia) and 12 introduced populations (from different countries). These accessions were analyzed for protein, oil, starch and tryptophan contents, in order to identify drought tolerant accessions with high grain quality. Also, simple sequence repeat (SSR) analysis with specific primers for opaque2 recessive allele (o2) was carried out. All analyzed accessions showed high levels of protein. Oil content ranged from 3.75 % to 5.40 % and starch content from 67.5 % to 71.30%. Average protein content was not different (p  lt  0.01) between landraces and introduced populations. Starch and oil contents were higher in introduced populations at 0.84 % and 0.39 %, respectively (p  lt  0.01). Twenty-three accessions had high levels of tryptophan content. A high percentage of kernel type 1 and 2 indicated the presence of endosperm hardness modifier genes. Recessive o2 allele was found in most of the accessions. Absence of o2 in some high tryptophan accessions indicated action of another mutation. In two high tryptophan accessions an unknown band was detected. Absence of negative correlations between proteins, tryptophan and oil makes certain accessions suitable for use in the simultaneous improvement of target genotypes for these traits. Identified drought tolerant, high quality accessions can be used in breeding programs aimed at nutritional improvement of maize grown under drought conditions.",
publisher = "Univ Sao Paolo, Cerquera Cesar",
journal = "Scientia Agricola",
title = "High grain quality accessions within a maize drought tolerant core collection",
volume = "71",
number = "5",
pages = "402-409",
doi = "10.1590/0103-9016-2013-0112"
}

Ignjatović-Micić, D., Kostadinović, M., Božinović, S., Anđelković, V.,& Vančetović, J.. (2014). High grain quality accessions within a maize drought tolerant core collection. in Scientia Agricola
Univ Sao Paolo, Cerquera Cesar., 71(5), 402-409.
https://doi.org/10.1590/0103-9016-2013-0112

Ignjatović-Micić D, Kostadinović M, Božinović S, Anđelković V, Vančetović J. High grain quality accessions within a maize drought tolerant core collection. in Scientia Agricola. 2014;71(5):402-409.
doi:10.1590/0103-9016-2013-0112 .

Ignjatović-Micić, Dragana, Kostadinović, Marija, Božinović, Sofija, Anđelković, Violeta, Vančetović, Jelena, "High grain quality accessions within a maize drought tolerant core collection" in Scientia Agricola, 71, no. 5 (2014):402-409,
https://doi.org/10.1590/0103-9016-2013-0112 . .

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Anđelković, Violeta
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/514
AB  - Quality protein maize (QPM) is high lysine (tryptophan) maize with hard endosperm and good agronomic performance. QPM was developed primarily for utilization in tropical and sub-tropical regions where maize is a staple food. Its adaptation and cultivation in temperate areas is still not fully developed, although QPM could have merits for production and consumption in developed parts of the world, especially as animal feed. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The objective of our research was initial screening of 72 hybrids (derived from crosses between QPM lines adapted to temperate environments and three commercial lines with standard kernel quality) for kernel modification, tryptophan and protein contents, quality index (QI) and grain yield. Five hybrids with high tryptophan content (0.071 to 0.081%) and yield at the level of standard hybrids (96 - 114%) were identified. Protein content ranged from 10 to 11.20%, similar to standard hybrids. QI was in the range from 0.71 to 0.74, which was better than in standard hybrids (0.57 - 0.62), but below the QPM threshold of 0.80. The percentage of good kernel modifications (type 1 and 2) was over 80% in three and over 74% in two hybrids. The results indicated the complexity of obtaining high yielding hybrids with high levels of essential amino-acids. Identified potential QPM hybrids have to be further evaluated for agronomic traits, but the results can be considered important in the context of limited information on QPM adapted to temperate environmental conditions.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions
VL  - 58
IS  - 1-4
SP  - 311
EP  - 317
UR  - https://hdl.handle.net/21.15107/rcub_rik_514
ER  - 

@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Stanković, Goran and Marković, Ksenija and Vančetović, Jelena and Božinović, Sofija and Anđelković, Violeta",
year = "2013",
abstract = "Quality protein maize (QPM) is high lysine (tryptophan) maize with hard endosperm and good agronomic performance. QPM was developed primarily for utilization in tropical and sub-tropical regions where maize is a staple food. Its adaptation and cultivation in temperate areas is still not fully developed, although QPM could have merits for production and consumption in developed parts of the world, especially as animal feed. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The objective of our research was initial screening of 72 hybrids (derived from crosses between QPM lines adapted to temperate environments and three commercial lines with standard kernel quality) for kernel modification, tryptophan and protein contents, quality index (QI) and grain yield. Five hybrids with high tryptophan content (0.071 to 0.081%) and yield at the level of standard hybrids (96 - 114%) were identified. Protein content ranged from 10 to 11.20%, similar to standard hybrids. QI was in the range from 0.71 to 0.74, which was better than in standard hybrids (0.57 - 0.62), but below the QPM threshold of 0.80. The percentage of good kernel modifications (type 1 and 2) was over 80% in three and over 74% in two hybrids. The results indicated the complexity of obtaining high yielding hybrids with high levels of essential amino-acids. Identified potential QPM hybrids have to be further evaluated for agronomic traits, but the results can be considered important in the context of limited information on QPM adapted to temperate environmental conditions.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions",
volume = "58",
number = "1-4",
pages = "311-317",
url = "https://hdl.handle.net/21.15107/rcub_rik_514"
}

Ignjatović-Micić, D., Kostadinović, M., Stanković, G., Marković, K., Vančetović, J., Božinović, S.,& Anđelković, V.. (2013). Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions. in Maydica
Maydica-Ist Sper Cerealicoltur, Bergamo., 58(1-4), 311-317.
https://hdl.handle.net/21.15107/rcub_rik_514

Ignjatović-Micić D, Kostadinović M, Stanković G, Marković K, Vančetović J, Božinović S, Anđelković V. Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions. in Maydica. 2013;58(1-4):311-317.
https://hdl.handle.net/21.15107/rcub_rik_514 .

Ignjatović-Micić, Dragana, Kostadinović, Marija, Stanković, Goran, Marković, Ksenija, Vančetović, Jelena, Božinović, Sofija, Anđelković, Violeta, "Biochemical and agronomic performance of quality protein maize hybrids adapted to temperate regions" in Maydica, 58, no. 1-4 (2013):311-317,
https://hdl.handle.net/21.15107/rcub_rik_514 .

TY  - JOUR
AU  - Mladenović Drinić, Snežana
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Simić, Milena
AU  - Stefanović, Lidija
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/968
AB  - Two populations of Amaranthus retroflexus with different morphology were collected from field of the Maize Research Institute Zemun Polje, Serbia. Random amplified polymorphic DNA (RAPD) and seed protein analysis were performed to study the genetic differences in two grain Amaranthus populations. The studied populations have different protein and DNA profile. A total of 171 DNA fragments were generated by 31 RAPD primers, with an average of 5.5 fragments per primer. Of these, 61.4% fragments were polymorphic among the two populations. 18 protein fraction were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The populations differed in the four protein fractions of different molecular weight. The seed protein electrophoresis and RAPD markers are useful for genetic determination of A. retroflexus populations and identification of biotypes with atypical morphology.
PB  - Nigeria : Academic Journals
T2  - African Journal of Biotechnology
T1  - Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers
VL  - 11
IS  - 29
SP  - 7331
EP  - 7337
DO  - 10.5897/AJB11.1254
ER  - 

@article{
author = "Mladenović Drinić, Snežana and Kostadinović, Marija and Ristić, Danijela and Simić, Milena and Stefanović, Lidija",
year = "2012",
abstract = "Two populations of Amaranthus retroflexus with different morphology were collected from field of the Maize Research Institute Zemun Polje, Serbia. Random amplified polymorphic DNA (RAPD) and seed protein analysis were performed to study the genetic differences in two grain Amaranthus populations. The studied populations have different protein and DNA profile. A total of 171 DNA fragments were generated by 31 RAPD primers, with an average of 5.5 fragments per primer. Of these, 61.4% fragments were polymorphic among the two populations. 18 protein fraction were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The populations differed in the four protein fractions of different molecular weight. The seed protein electrophoresis and RAPD markers are useful for genetic determination of A. retroflexus populations and identification of biotypes with atypical morphology.",
publisher = "Nigeria : Academic Journals",
journal = "African Journal of Biotechnology",
title = "Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers",
volume = "11",
number = "29",
pages = "7331-7337",
doi = "10.5897/AJB11.1254"
}

Mladenović Drinić, S., Kostadinović, M., Ristić, D., Simić, M.,& Stefanović, L.. (2012). Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers. in African Journal of Biotechnology
Nigeria : Academic Journals., 11(29), 7331-7337.
https://doi.org/10.5897/AJB11.1254

Mladenović Drinić S, Kostadinović M, Ristić D, Simić M, Stefanović L. Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers. in African Journal of Biotechnology. 2012;11(29):7331-7337.
doi:10.5897/AJB11.1254 .

Mladenović Drinić, Snežana, Kostadinović, Marija, Ristić, Danijela, Simić, Milena, Stefanović, Lidija, "Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers" in African Journal of Biotechnology, 11, no. 29 (2012):7331-7337,
https://doi.org/10.5897/AJB11.1254 . .

TY  - JOUR
AU  - Mladenović Drinić, Snežana
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Stevanović, Milan
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Kovačević, Dragan
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/444
AB  - The yield, grain yield heterosis and genetic distance based on SSR markers were analyzed in eight maize hybrids and their parental lines. The mean grain yield of all F1 hybrids was 11.37 tha-1. The F1 hybrid from the crosses between L4xL6 gave the highest grain yield of 12.12 tha-1. For the mid parent heterosis (MPH) grain yields of the F1 hybrids, the data showed the average value of 164.25%, and ranged from 136.72% (L4xL6) to 218.07% (L8xL2), and for better parent heterosis (BPH) from 100.70% (H4) to 212.60% (H2), averaged 137.36%. The average genetic distance among parental inbred lines of analyzed hybrids was 0.58 with a range from 0.55 to 0.61. The GD showed a positive correlation with the grain yield of the F1 hybrids (0.22), as well as with MPH and BPH, with the values of 0.12 and 0.45, respectively.
AB  - Prinos, heterozis za prinos zrna i genetička distanca izračunata na osnovu SSR markera, su ispitivani za osam hibrida kukuruza i njihove roditeljske komponente. Prosecan prinos zrna F1 hibrida je bio 11.37 tha-1. Hibrid dobijen ukrštanjem linija L4xL6 je imao najveći prinos od 12.12 tha-1. Prosečna vrednost heterozisa u odnosu na prosečnog roditelja za prinos zrna za hibride je bila 164.25%, i varirala je od 136.72% (L4xL6) do 218.07% (L8xL2), i za heterozis u odnosu na boljeg roditelja od 100.70% (H4) do 212.60% (H2), prosečno 137.36%. Prosečna genetička distanca između roditeljskih linija ispitanih hibrida je bila 0.58 sa opsegom od 0.55 do 0.61. Korelacija između GD i prinosa hibrida je bila pozitivna (r=0.22), kao i sa heterozisom u odnosu na prosečnog roditelja (r=0.12) odnosno heterozisom u odnosu na boljeg roditelja (r=0.45).
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Correlation of yield and heterosis of maize hybrids and their parental lines with genetic distance based on SSR markers
T1  - Korelacija prinosa i heterozisa hibrida kukuruza i njihovih roditeljskih linija sa genetičkom distancom na osnovu SSR markera
VL  - 44
IS  - 2
SP  - 399
EP  - 408
DO  - 10.2298/GENSR1202399D
ER  - 

@article{
author = "Mladenović Drinić, Snežana and Kostadinović, Marija and Ristić, Danijela and Stevanović, Milan and Čamdžija, Zoran and Filipović, Milomir and Kovačević, Dragan",
year = "2012",
abstract = "The yield, grain yield heterosis and genetic distance based on SSR markers were analyzed in eight maize hybrids and their parental lines. The mean grain yield of all F1 hybrids was 11.37 tha-1. The F1 hybrid from the crosses between L4xL6 gave the highest grain yield of 12.12 tha-1. For the mid parent heterosis (MPH) grain yields of the F1 hybrids, the data showed the average value of 164.25%, and ranged from 136.72% (L4xL6) to 218.07% (L8xL2), and for better parent heterosis (BPH) from 100.70% (H4) to 212.60% (H2), averaged 137.36%. The average genetic distance among parental inbred lines of analyzed hybrids was 0.58 with a range from 0.55 to 0.61. The GD showed a positive correlation with the grain yield of the F1 hybrids (0.22), as well as with MPH and BPH, with the values of 0.12 and 0.45, respectively., Prinos, heterozis za prinos zrna i genetička distanca izračunata na osnovu SSR markera, su ispitivani za osam hibrida kukuruza i njihove roditeljske komponente. Prosecan prinos zrna F1 hibrida je bio 11.37 tha-1. Hibrid dobijen ukrštanjem linija L4xL6 je imao najveći prinos od 12.12 tha-1. Prosečna vrednost heterozisa u odnosu na prosečnog roditelja za prinos zrna za hibride je bila 164.25%, i varirala je od 136.72% (L4xL6) do 218.07% (L8xL2), i za heterozis u odnosu na boljeg roditelja od 100.70% (H4) do 212.60% (H2), prosečno 137.36%. Prosečna genetička distanca između roditeljskih linija ispitanih hibrida je bila 0.58 sa opsegom od 0.55 do 0.61. Korelacija između GD i prinosa hibrida je bila pozitivna (r=0.22), kao i sa heterozisom u odnosu na prosečnog roditelja (r=0.12) odnosno heterozisom u odnosu na boljeg roditelja (r=0.45).",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Correlation of yield and heterosis of maize hybrids and their parental lines with genetic distance based on SSR markers, Korelacija prinosa i heterozisa hibrida kukuruza i njihovih roditeljskih linija sa genetičkom distancom na osnovu SSR markera",
volume = "44",
number = "2",
pages = "399-408",
doi = "10.2298/GENSR1202399D"
}

Mladenović Drinić, S., Kostadinović, M., Ristić, D., Stevanović, M., Čamdžija, Z., Filipović, M.,& Kovačević, D.. (2012). Correlation of yield and heterosis of maize hybrids and their parental lines with genetic distance based on SSR markers. in Genetika
Društvo genetičara Srbije, Beograd., 44(2), 399-408.
https://doi.org/10.2298/GENSR1202399D

Mladenović Drinić S, Kostadinović M, Ristić D, Stevanović M, Čamdžija Z, Filipović M, Kovačević D. Correlation of yield and heterosis of maize hybrids and their parental lines with genetic distance based on SSR markers. in Genetika. 2012;44(2):399-408.
doi:10.2298/GENSR1202399D .

Mladenović Drinić, Snežana, Kostadinović, Marija, Ristić, Danijela, Stevanović, Milan, Čamdžija, Zoran, Filipović, Milomir, Kovačević, Dragan, "Correlation of yield and heterosis of maize hybrids and their parental lines with genetic distance based on SSR markers" in Genetika, 44, no. 2 (2012):399-408,
https://doi.org/10.2298/GENSR1202399D . .

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Mladenović Drinić, Snežana
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/440
AB  - Protein quality of standard maize kernels is poor, due to the low levels of two essential aminoacids - lysine and tryptophan. The opaque2 mutation which increases lysine and tryptophan content also confers an undesirable phenotype leading to low yields, soft and chalky kernels that renders seeds susceptible to storage pest and ear rots. Quality protein maize (QPM) is the maize with increased levels of these amino acids and good agronomic performances. At the Maize Research Institute, a program on creating QPM germplasm developed for conditions of temperate climatic zone is in progress. The aim of this study was to test quality traits of F2 kernels derived from the crosses between 24 QPM inbred lines with three standard inbred lines and two opaque2 lines. Most of the analyzed 108 genotypes had tryptophan content under the threshold of 0.075 set for QPM (83.5%). However, 50% had elevated tryptophan content in the range from 0.065 to 0.074. High tryptophan content was found in 16.5% of the analyzed genotypes, the highest being 0.088. Only one genotype had quality index over 0.80 (threshold for QPM). Biochemical analysis identified genotypes with good protein quality which will be further tested for agronomic performances in order to potentially be considered as QPM hybrids.
AB  - Proteini kukuruza su siromašni u sadržaju dve esencijalne aminokiseline - lizina i triptofana. Prirodna opaque2 mutacija udvostručava nivo ovih aminokiselina, ali istovremeno smanjuje prinos, čini endosperm kukuruza mekim i brašnjavim, a seme osetljivim na trulež klipa i štetočine skladištenog zrna. Kukuruz visokog kvaliteta proteina (Quality Protein Maize - QPM) se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma i istovremeno dobrim agronomskim performansama. U Institutu za kukuruz već nekoliko godina postoji program stvaranja genotipova kukuruza visokog kvaliteta proteina, adaptiranih na umereno klimatsko područje. Cilj ovog rada je bio da se ispita kvalitet proteina F2 zrna kukuruza dobijenog ukrštanjem 24 QPM inbred linija sa tri standardne inbred linije i dve opaque2 linije. Većina od 108 analiziranih genotipova (83,5%) je imala sadržaj triptofana ispod praga od 0,075, od čega je poboljšani nivo triptofana (0,065-0,074) imalo 50% genotipova. Visok sadržaj je imalo 16,5% analiziranih genotipova, od kojih je najviša vrednost bila 0,088. Samo jedan genotip je imao indeks kvaliteta proteina iznad praga od 0,80. Biohemijskom analizom utvrđeni su genotipovi poboljšanog kvaliteta čija će agronomska svojstva biti ispitana kako bi se utvrdilo da li se mogu smatrati potencijalnim QPM hibridima.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Protein quality analysis of F2 maize kernels
T1  - Biohemijska analiza F2 zrna genotipova kukuruza visokog kvaliteta proteina
VL  - 18
IS  - 1
SP  - 33
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_rik_440
ER  - 

@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Mladenović Drinić, Snežana",
year = "2012",
abstract = "Protein quality of standard maize kernels is poor, due to the low levels of two essential aminoacids - lysine and tryptophan. The opaque2 mutation which increases lysine and tryptophan content also confers an undesirable phenotype leading to low yields, soft and chalky kernels that renders seeds susceptible to storage pest and ear rots. Quality protein maize (QPM) is the maize with increased levels of these amino acids and good agronomic performances. At the Maize Research Institute, a program on creating QPM germplasm developed for conditions of temperate climatic zone is in progress. The aim of this study was to test quality traits of F2 kernels derived from the crosses between 24 QPM inbred lines with three standard inbred lines and two opaque2 lines. Most of the analyzed 108 genotypes had tryptophan content under the threshold of 0.075 set for QPM (83.5%). However, 50% had elevated tryptophan content in the range from 0.065 to 0.074. High tryptophan content was found in 16.5% of the analyzed genotypes, the highest being 0.088. Only one genotype had quality index over 0.80 (threshold for QPM). Biochemical analysis identified genotypes with good protein quality which will be further tested for agronomic performances in order to potentially be considered as QPM hybrids., Proteini kukuruza su siromašni u sadržaju dve esencijalne aminokiseline - lizina i triptofana. Prirodna opaque2 mutacija udvostručava nivo ovih aminokiselina, ali istovremeno smanjuje prinos, čini endosperm kukuruza mekim i brašnjavim, a seme osetljivim na trulež klipa i štetočine skladištenog zrna. Kukuruz visokog kvaliteta proteina (Quality Protein Maize - QPM) se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma i istovremeno dobrim agronomskim performansama. U Institutu za kukuruz već nekoliko godina postoji program stvaranja genotipova kukuruza visokog kvaliteta proteina, adaptiranih na umereno klimatsko područje. Cilj ovog rada je bio da se ispita kvalitet proteina F2 zrna kukuruza dobijenog ukrštanjem 24 QPM inbred linija sa tri standardne inbred linije i dve opaque2 linije. Većina od 108 analiziranih genotipova (83,5%) je imala sadržaj triptofana ispod praga od 0,075, od čega je poboljšani nivo triptofana (0,065-0,074) imalo 50% genotipova. Visok sadržaj je imalo 16,5% analiziranih genotipova, od kojih je najviša vrednost bila 0,088. Samo jedan genotip je imao indeks kvaliteta proteina iznad praga od 0,80. Biohemijskom analizom utvrđeni su genotipovi poboljšanog kvaliteta čija će agronomska svojstva biti ispitana kako bi se utvrdilo da li se mogu smatrati potencijalnim QPM hibridima.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Protein quality analysis of F2 maize kernels, Biohemijska analiza F2 zrna genotipova kukuruza visokog kvaliteta proteina",
volume = "18",
number = "1",
pages = "33-39",
url = "https://hdl.handle.net/21.15107/rcub_rik_440"
}

Kostadinović, M., Ignjatović-Micić, D., Stanković, G.,& Mladenović Drinić, S.. (2012). Protein quality analysis of F2 maize kernels. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 18(1), 33-39.
https://hdl.handle.net/21.15107/rcub_rik_440

Kostadinović M, Ignjatović-Micić D, Stanković G, Mladenović Drinić S. Protein quality analysis of F2 maize kernels. in Selekcija i semenarstvo. 2012;18(1):33-39.
https://hdl.handle.net/21.15107/rcub_rik_440 .

Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Mladenović Drinić, Snežana, "Protein quality analysis of F2 maize kernels" in Selekcija i semenarstvo, 18, no. 1 (2012):33-39,
https://hdl.handle.net/21.15107/rcub_rik_440 .

TY  - JOUR
AU  - Ivetić, Vladan
AU  - Isajev, Vasilije
AU  - Nikolić, Ana
AU  - Krstić, Milun
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/457
AB  - The results of spatial analysis of genetic diversity have practical application in the definition and delineation of regional provenances of forest trees. Research in this paper, was based on the material from 27 natural populations of beech in Serbia. The genetic component of the research is based on the analysis of RAPD markers from bulk samples, using 28 primers. The spatial component of the research is based on the geographical position of the studied populations. Grouping of the studied populations in the regions, as well as their separation, was performed using the Monmonier’s algorithm of maximum differences. To visualize the results and mapping the regions of beech provenances in Serbia, GIS was used, with database included the results of this study.
AB  - Rezultati prostorne analize genetičkog diverziteta imaju praktičnu primenu u definisanju i razgraničenju regiona provenijencija šumskih vrsta drveća. Istraživanja u ovom radu, obuhvatila su materijal iz 27 prirodnih populacija bukve u Srbiji. Genetička komponenta istraživanja bazira se na analizi RAPD markera iz zbirnih uzoraka, korišćenjem 28 prajmera. Prostorna komponenta istraživanja, bazira se na geografskom položaju ispitivanih populacija. Grupisanje ispitivanih populacija u regione, kao i njihovo razgraničenje, izvršeno je primenom Monmonierovog algoritma najvećih razlika. Za vizualizaciju rezultata i izradu karata regiona provenijencija bukve u Srbiji, korišćen je GIS, čija je baza podataka obuhvatila rezultate navedenih istraživanja.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Delineation of beech provenance regions in Serbia by spatial analysis of genetic diversity
T1  - Izdvajanje regiona provenijencija bukve u Srbiji prostornom analizom genetičkog diverziteta
VL  - 44
IS  - 1
SP  - 101
EP  - 108
DO  - 10.2298/GENSR1201101I
ER  - 

@article{
author = "Ivetić, Vladan and Isajev, Vasilije and Nikolić, Ana and Krstić, Milun and Ristić, Danijela and Kostadinović, Marija",
year = "2012",
abstract = "The results of spatial analysis of genetic diversity have practical application in the definition and delineation of regional provenances of forest trees. Research in this paper, was based on the material from 27 natural populations of beech in Serbia. The genetic component of the research is based on the analysis of RAPD markers from bulk samples, using 28 primers. The spatial component of the research is based on the geographical position of the studied populations. Grouping of the studied populations in the regions, as well as their separation, was performed using the Monmonier’s algorithm of maximum differences. To visualize the results and mapping the regions of beech provenances in Serbia, GIS was used, with database included the results of this study., Rezultati prostorne analize genetičkog diverziteta imaju praktičnu primenu u definisanju i razgraničenju regiona provenijencija šumskih vrsta drveća. Istraživanja u ovom radu, obuhvatila su materijal iz 27 prirodnih populacija bukve u Srbiji. Genetička komponenta istraživanja bazira se na analizi RAPD markera iz zbirnih uzoraka, korišćenjem 28 prajmera. Prostorna komponenta istraživanja, bazira se na geografskom položaju ispitivanih populacija. Grupisanje ispitivanih populacija u regione, kao i njihovo razgraničenje, izvršeno je primenom Monmonierovog algoritma najvećih razlika. Za vizualizaciju rezultata i izradu karata regiona provenijencija bukve u Srbiji, korišćen je GIS, čija je baza podataka obuhvatila rezultate navedenih istraživanja.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Delineation of beech provenance regions in Serbia by spatial analysis of genetic diversity, Izdvajanje regiona provenijencija bukve u Srbiji prostornom analizom genetičkog diverziteta",
volume = "44",
number = "1",
pages = "101-108",
doi = "10.2298/GENSR1201101I"
}

Ivetić, V., Isajev, V., Nikolić, A., Krstić, M., Ristić, D.,& Kostadinović, M.. (2012). Delineation of beech provenance regions in Serbia by spatial analysis of genetic diversity. in Genetika
Društvo genetičara Srbije, Beograd., 44(1), 101-108.
https://doi.org/10.2298/GENSR1201101I

Ivetić V, Isajev V, Nikolić A, Krstić M, Ristić D, Kostadinović M. Delineation of beech provenance regions in Serbia by spatial analysis of genetic diversity. in Genetika. 2012;44(1):101-108.
doi:10.2298/GENSR1201101I .

Ivetić, Vladan, Isajev, Vasilije, Nikolić, Ana, Krstić, Milun, Ristić, Danijela, Kostadinović, Marija, "Delineation of beech provenance regions in Serbia by spatial analysis of genetic diversity" in Genetika, 44, no. 1 (2012):101-108,
https://doi.org/10.2298/GENSR1201101I . .