Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize

2014
Authors
Kostadinović, Marija
Ignjatović-Micić, Dragana

Stanković, Goran

Vančetović, Jelena

Ristić, Danijela

Mladenović-Drinić, Snežana

Article (Published version)
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Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus c...an be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.
Keywords:
maize / marker assisted selection / opaque2 / Quality Protein Maize / SSRSource:
Genetika, 2014, 46, 2, 579-590Publisher:
- Društvo genetičara Srbije, Beograd
Funding / projects:
DOI: 10.2298/GENSR1402579K
ISSN: 0534-0012
WoS: 000342659500024
Scopus: 2-s2.0-84968739652
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MRIZPTY - JOUR AU - Kostadinović, Marija AU - Ignjatović-Micić, Dragana AU - Stanković, Goran AU - Vančetović, Jelena AU - Ristić, Danijela AU - Mladenović-Drinić, Snežana PY - 2014 UR - http://rik.mrizp.rs/handle/123456789/526 AB - Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize VL - 46 IS - 2 SP - 579 EP - 590 DO - 10.2298/GENSR1402579K UR - conv_888 ER -
@article{ author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović-Drinić, Snežana", year = "2014", abstract = "Maize protein is deficient in two essential amino acids, lysine and tryptophan. Naturally occurring opaque2 (o2) mutation which increases the levels of tryptophan and lysine in the grain also confers an undesirable phenotype leading to low yields and more susceptible, soft and chalky kernels. Quality Protein Maize (QPM) is agronomically acceptable and nutritionally improved opaque2 maize. Marker assisted selection increases reliability and efficiency, reduces time and costs taken to obtain QPM. The results presented in this paper are a part of the Maize Research Insitute project on conversion of normal maize lines to QPM lines adapted for growing in temperate regions trough marker assisted backcross (MAB). Genetic and biochemical variability was analyzed between normal (ZPL 5) and QPM (CML 144) parental inbred lines and the efficiency of the three opaque2 specific SSR markers was examined. Markers phi057 and umc1066 can discriminate homozygous and heterozygous backcross progeny, thus can be used as foreground selection markers for the opaque2 gene. The genetic similarity between analyzed lines was 0.05, which confirmed good selection of parental lines for the creation of hybrids. Tryptophan content was 0.093 and QI was 0.85 in CML 144, approximately twice as high compared to 0.054 and 0.48 in ZPL 5. Both tryptophan and QI values for CML 144 were above the QPM treshold, i.e. tryptophan for 24% and QI for 0.625%, making it a suitable donor line of o2. These results represent the framework for marker assisted introgression of the quality protein trait into local maize genotype.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize", volume = "46", number = "2", pages = "579-590", doi = "10.2298/GENSR1402579K", url = "conv_888" }
Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović-Drinić, S.. (2014). Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika Društvo genetičara Srbije, Beograd., 46(2), 579-590. https://doi.org/10.2298/GENSR1402579K conv_888
Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović-Drinić S. Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize. in Genetika. 2014;46(2):579-590. doi:10.2298/GENSR1402579K conv_888 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović-Drinić, Snežana, "Genetic and biochemical characterization of parental inbred lines in marker assisted selection for quality protein maize" in Genetika, 46, no. 2 (2014):579-590, https://doi.org/10.2298/GENSR1402579K ., conv_888 .