TY  - CONF
AU  - Petrović, Tanja
AU  - Milivojević, Marija
AU  - Branković-Radojčić, Dragana V.
AU  - Jovanović, Snežana
AU  - Vujinović, Jasna
AU  - Vukadinović, Radmila
AU  - Stojadinović-Životić, Jasmina
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/953
AB  - Seed vigor is the quality parameter which indicates germination under wide range of 
environmental conditions. This trait can be identified by standard germination test as so-called 
first count germination or germination energy. In addition, several tests for vigor evaluation 
were adopted by seed testing associations such as ISTA and AOSA. The main purpose for 
developing additional vigor tests is to obtain fast and reliable information on seed vigor which 
contributes to decision making in the inventory management. ISTA rules prescribes several 
tests for evaluating seed vigor, namely: conductivity test, accelerated ageing (AA) for soybean, 
controlled deterioration for Brassica seeds, Radicle emergence (RE) and Tetrazolium (TZ) 
vigor test for soybean. Seed testing laboratory of the Maize Research Institute is accredited by 
ISTA for RE vigor test, has internally developed cold-test and is capable for application of TZ 
vigor test for research purposes. These tests were applied on several maize seeds lots with 
declining germination energy and still highly rated final germination. Results of vigor tests 
were correlated with the standard germination quality parameters, germination energy and final 
count, in order to identify vigor test which provides the most approximate output to the standard 
germination test. Correlations were significant for all vigor tests, in average 0.882 and 0.713 
for germination first and final count, respectively. The highest compatibility between vigor and 
germination tests was identified for cold test with correlation coefficients of 0.952 and 0.839 
for germination first and final count, respectively. However, due to duration of cold test (2 
weeks), it is not capable of providing fast information. Therefore, other two vigor tests, TZ and 
RE, which are shorter and have high correlation with germination energy (0.901 and 0.811, 
respectively) and final count (0.713 and 0.728, respectively) can be recommended for this 
purpose.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Identification of early decline of seed quality by vigor tests
SP  - 61
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_rik_953
ER  - 

@conference{
author = "Petrović, Tanja and Milivojević, Marija and Branković-Radojčić, Dragana V. and Jovanović, Snežana and Vujinović, Jasna and Vukadinović, Radmila and Stojadinović-Životić, Jasmina",
year = "2022",
abstract = "Seed vigor is the quality parameter which indicates germination under wide range of 
environmental conditions. This trait can be identified by standard germination test as so-called 
first count germination or germination energy. In addition, several tests for vigor evaluation 
were adopted by seed testing associations such as ISTA and AOSA. The main purpose for 
developing additional vigor tests is to obtain fast and reliable information on seed vigor which 
contributes to decision making in the inventory management. ISTA rules prescribes several 
tests for evaluating seed vigor, namely: conductivity test, accelerated ageing (AA) for soybean, 
controlled deterioration for Brassica seeds, Radicle emergence (RE) and Tetrazolium (TZ) 
vigor test for soybean. Seed testing laboratory of the Maize Research Institute is accredited by 
ISTA for RE vigor test, has internally developed cold-test and is capable for application of TZ 
vigor test for research purposes. These tests were applied on several maize seeds lots with 
declining germination energy and still highly rated final germination. Results of vigor tests 
were correlated with the standard germination quality parameters, germination energy and final 
count, in order to identify vigor test which provides the most approximate output to the standard 
germination test. Correlations were significant for all vigor tests, in average 0.882 and 0.713 
for germination first and final count, respectively. The highest compatibility between vigor and 
germination tests was identified for cold test with correlation coefficients of 0.952 and 0.839 
for germination first and final count, respectively. However, due to duration of cold test (2 
weeks), it is not capable of providing fast information. Therefore, other two vigor tests, TZ and 
RE, which are shorter and have high correlation with germination energy (0.901 and 0.811, 
respectively) and final count (0.713 and 0.728, respectively) can be recommended for this 
purpose.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Identification of early decline of seed quality by vigor tests",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_rik_953"
}

Petrović, T., Milivojević, M., Branković-Radojčić, D. V., Jovanović, S., Vujinović, J., Vukadinović, R.,& Stojadinović-Životić, J.. (2022). Identification of early decline of seed quality by vigor tests. in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 61-61.
https://hdl.handle.net/21.15107/rcub_rik_953

Petrović T, Milivojević M, Branković-Radojčić DV, Jovanović S, Vujinović J, Vukadinović R, Stojadinović-Životić J. Identification of early decline of seed quality by vigor tests. in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:61-61.
https://hdl.handle.net/21.15107/rcub_rik_953 .

Petrović, Tanja, Milivojević, Marija, Branković-Radojčić, Dragana V., Jovanović, Snežana, Vujinović, Jasna, Vukadinović, Radmila, Stojadinović-Životić, Jasmina, "Identification of early decline of seed quality by vigor tests" in 25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022):61-61,
https://hdl.handle.net/21.15107/rcub_rik_953 .

TY  - JOUR
AU  - Milivojević, Marija
AU  - Vujinović, Jasna
AU  - Branković-Radojčić, Dragana V.
AU  - Vukadinović, Radmila
AU  - Petrović, Tanja
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/899
AB  - Accuracy and random selection of seeds when testing the 1000 seeds weight in the laboratory is of great importance in order to ensure the representativeness of the sample and precise information for further packaging process. The aim of this research was to check whether the laboratory seed counter selects seeds by shape and size and whether there is a trend during operation at maximum and reduced speed. Ten species of different seed sizes were used. Ten replicates of 100 seeds were counted by counter, the number of seeds was manually checked and the weight measured. The analysis of the obtained results showed that the counter is precise, but weight results showed selection of smaller seeds (Capsicum annuum and Glycine max) at maximum counting speed. Trend of larger seed selection was observed in Medicago sativa and pelleted Beta vulgaris. Slower counting (50% of the maximum speed), is ensured precise counting without trends.
AB  - Praćenje i kontrola rada laboratorijske opreme je jedan od zahteva akreditacionih standarda za laboratorije za ispitivanje
semena. U dosadašnjoj praksi nije se obraćala pažnja da li prilikom rada brojača dolazi do selektivnog izbora semena koje se broji.
Preciznost i slučajan izbor semena kod ispitivanja mase 1000 semena u laboratoriji je od velike važnosti kako bi se obezbedila
reprezentativnost uzorka. Cilj ovog istraživanja je provera da li laboratorijski brojač semena u toku svog rada, pri maksimalnoj i
smanjenoj brzini selektuje seme po obliku i veličini i da li postoji trend. U radu je ispitivano 10 vrsta različite krupno će semena
(Capsicum annuum, peletirano seme Beta vulgaris, Brassica napus, Medicago sativa, Raphanus sativus, Sinapis alba, Triticum
aestivum, Helianthus annuus, Glycine max i Zea mays). Analizom dobijenih rezultata utvrđeno je da brojač precizno broji. Na osnovu
rezultata izmerene mase uočeno je da se na maksimalnoj brzini brojanja pojavljuje trend selektovanja prvo sitnijeg, a zatim krupnijeg
semena kod vrsta Capsicum annuum i Glycine max. Nasuprot tome, kod semena Medicago sativa i peletiranog semena Beta vulgaris,
uočen je trend selektovanja semena od krupnijeg ka sitnijem. Prilikom sporijeg brojanja (50% od maksimalne brzine), postiže se
slučajan odabir semena tj. ne pojavljuje se trend prilikom rada brojača, kod gore navedenih vrsta semena. Za ostale vrste nije uočen
trend postepenog smanjenja niti pove ćanja vrednosti mase u okviru ispitivanih 10 ponavljanja. Rezultati ovih analiza ukazuju na
važnost provere opreme kako u smislu tačnosti brojanja tako i selektovanja semena u cilju dobijanja tačnih rezultata ispitivanja mase
1000 semena.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
T2  - Journal on Processing and Energy in Agriculture
T1  - Monitoring of seed counter in 1000 seed weight testing
T1  - Provera brojača semena u ispitivanju mase 1000 semena
VL  - 26
IS  - 1
SP  - 34
EP  - 37
EP  - 
DO  - 10.5937/jpea26-36855
ER  - 

@article{
author = "Milivojević, Marija and Vujinović, Jasna and Branković-Radojčić, Dragana V. and Vukadinović, Radmila and Petrović, Tanja",
year = "2022",
abstract = "Accuracy and random selection of seeds when testing the 1000 seeds weight in the laboratory is of great importance in order to ensure the representativeness of the sample and precise information for further packaging process. The aim of this research was to check whether the laboratory seed counter selects seeds by shape and size and whether there is a trend during operation at maximum and reduced speed. Ten species of different seed sizes were used. Ten replicates of 100 seeds were counted by counter, the number of seeds was manually checked and the weight measured. The analysis of the obtained results showed that the counter is precise, but weight results showed selection of smaller seeds (Capsicum annuum and Glycine max) at maximum counting speed. Trend of larger seed selection was observed in Medicago sativa and pelleted Beta vulgaris. Slower counting (50% of the maximum speed), is ensured precise counting without trends., Praćenje i kontrola rada laboratorijske opreme je jedan od zahteva akreditacionih standarda za laboratorije za ispitivanje
semena. U dosadašnjoj praksi nije se obraćala pažnja da li prilikom rada brojača dolazi do selektivnog izbora semena koje se broji.
Preciznost i slučajan izbor semena kod ispitivanja mase 1000 semena u laboratoriji je od velike važnosti kako bi se obezbedila
reprezentativnost uzorka. Cilj ovog istraživanja je provera da li laboratorijski brojač semena u toku svog rada, pri maksimalnoj i
smanjenoj brzini selektuje seme po obliku i veličini i da li postoji trend. U radu je ispitivano 10 vrsta različite krupno će semena
(Capsicum annuum, peletirano seme Beta vulgaris, Brassica napus, Medicago sativa, Raphanus sativus, Sinapis alba, Triticum
aestivum, Helianthus annuus, Glycine max i Zea mays). Analizom dobijenih rezultata utvrđeno je da brojač precizno broji. Na osnovu
rezultata izmerene mase uočeno je da se na maksimalnoj brzini brojanja pojavljuje trend selektovanja prvo sitnijeg, a zatim krupnijeg
semena kod vrsta Capsicum annuum i Glycine max. Nasuprot tome, kod semena Medicago sativa i peletiranog semena Beta vulgaris,
uočen je trend selektovanja semena od krupnijeg ka sitnijem. Prilikom sporijeg brojanja (50% od maksimalne brzine), postiže se
slučajan odabir semena tj. ne pojavljuje se trend prilikom rada brojača, kod gore navedenih vrsta semena. Za ostale vrste nije uočen
trend postepenog smanjenja niti pove ćanja vrednosti mase u okviru ispitivanih 10 ponavljanja. Rezultati ovih analiza ukazuju na
važnost provere opreme kako u smislu tačnosti brojanja tako i selektovanja semena u cilju dobijanja tačnih rezultata ispitivanja mase
1000 semena.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad : National Society of Processing and Energy in Agriculture",
journal = "Journal on Processing and Energy in Agriculture",
title = "Monitoring of seed counter in 1000 seed weight testing, Provera brojača semena u ispitivanju mase 1000 semena",
volume = "26",
number = "1",
pages = "34-37-",
doi = "10.5937/jpea26-36855"
}

Milivojević, M., Vujinović, J., Branković-Radojčić, D. V., Vukadinović, R.,& Petrović, T.. (2022). Monitoring of seed counter in 1000 seed weight testing. in Journal on Processing and Energy in Agriculture
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi., 26(1), 34-37.
https://doi.org/10.5937/jpea26-36855

Milivojević M, Vujinović J, Branković-Radojčić DV, Vukadinović R, Petrović T. Monitoring of seed counter in 1000 seed weight testing. in Journal on Processing and Energy in Agriculture. 2022;26(1):34-37.
doi:10.5937/jpea26-36855 .

Milivojević, Marija, Vujinović, Jasna, Branković-Radojčić, Dragana V., Vukadinović, Radmila, Petrović, Tanja, "Monitoring of seed counter in 1000 seed weight testing" in Journal on Processing and Energy in Agriculture, 26, no. 1 (2022):34-37,
https://doi.org/10.5937/jpea26-36855 . .

TY  - JOUR
AU  - Milivojević, Marija
AU  - Branković-Radojčić, Dragana V.
AU  - Vukadinović, Radmila
AU  - Kojić, Jasna
AU  - Dumanović, Zoran
AU  - Petrović, Tanja
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/1119
AB  - High germination of seeds and seedling establishment are critical for soybean production 
since it determines crop density and eventually affects the yield. Accurate evaluation of the ger mination potential of sowing material is valuable, as well as initial quality assessment of newly 
harvested seed. ISTA Rules prescribe alternative methods for soybean seed germination: sand, 
between paper, top of paper covered with sand and since 2020 organic growing media. In the 
Seed Testing Laboratory at the Maize Research Institute soybean germination is conducted in the 
sand at 20<=>30°C. The objective of this study was to evaluate several available organic growing 
media and compare results obtained with the standard laboratory method. Five soybean seed lots 
of different quality were tested in four germination growing media (3 organic and sand), at two 
temperature regimes (25 °C and 20<=>30°C). Statistical analyses showed that all types of organic 
growing media were of the same quality. Slightly higher germination in organic growing media 
was obtained in the experiment at 20<=>30°C compared to sand, but those differences were not 
statistically significant. When testing was done in germinator with 25°C positive effects of organic 
growing media were identified and this impact was significant, especially for low-quality seed 
lots. The experimental outcome was to continue with routine practice and use organic growing 
media for retesting of seed lots with lower germination as an alternative substrate.
Key words: germination, organic substrate, sand, soybean, temperature
AB  - Visoka klijavost semena i uniforman rast klijanaca su presudni za proizvodnju soje, jer od 
toga zavisi gustina useva i na kraju, prinos. Precizna procena potencijala klijavosti setvenog ma terijala je izuzetno važna, kao i početna procena kvaliteta tek požnjevenog semena. Međunarod na pravila za ispitivanje semena propisuju alternativne metode za klijanje semena soje: pesak, 
između filter papira, na filter papiru uz prekrivanje peskom, i od 2020. godine organski supstrat.
U Laboratoriji za ispitivanje semena, Instituta za kukuruz “Zemun Polje”, naklijavanje soje se 
vrši u pesku, na temperaturi 20<=>30°C. Cilj ove studije bio je proceniti nekoliko dostupnih 
organskih supstrata i uporediti dobijene rezultate sa dosadašnjom laboratorijskom metodom.
Pet partija soje različitog kvaliteta testirano je u četiri podloge za naklijavanje (3 organske i pe sak), pri dva temperaturna režima (25°C i 20<=>30°C). Statistička analiza pokazala je da su sve 
organske podloge istog kvaliteta. Nešto veća klijavost u organskom supstratu u poređenju sa pes kom je ostvarena u eksperimentu na 20<=>30°C, ali te razlike nisu bile statistički značajne. Pri likom ispitivanja u klijalištu sa 25°C pokazali su se pozitivni efekti organske podloge i ovaj uticaj 
je bio značajan, posebno za partije slabijeg kvaliteta. Nakon analize eksperimentalnih rezultata 
odlučeno je da se nastavi sa dosadašnjom laboratorijskom praksom naklijavanja u pesku i da se 
organska podloga koristi kao alternativni supstrat za ponovno ispitivanje partija semena sa nižim 
procentom klijavosti.
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Experimental evaluation of organic substrates for germination testing of soybean seed
T1  - Eksperimentalna procena organskih podloga za ispitivanje klijavosti semena soje
VL  - 26
IS  - 2
SP  - 1
EP  - 6
DO  - 10.5937/SelSem2002001M
ER  - 

@article{
author = "Milivojević, Marija and Branković-Radojčić, Dragana V. and Vukadinović, Radmila and Kojić, Jasna and Dumanović, Zoran and Petrović, Tanja",
year = "2020",
abstract = "High germination of seeds and seedling establishment are critical for soybean production 
since it determines crop density and eventually affects the yield. Accurate evaluation of the ger mination potential of sowing material is valuable, as well as initial quality assessment of newly 
harvested seed. ISTA Rules prescribe alternative methods for soybean seed germination: sand, 
between paper, top of paper covered with sand and since 2020 organic growing media. In the 
Seed Testing Laboratory at the Maize Research Institute soybean germination is conducted in the 
sand at 20<=>30°C. The objective of this study was to evaluate several available organic growing 
media and compare results obtained with the standard laboratory method. Five soybean seed lots 
of different quality were tested in four germination growing media (3 organic and sand), at two 
temperature regimes (25 °C and 20<=>30°C). Statistical analyses showed that all types of organic 
growing media were of the same quality. Slightly higher germination in organic growing media 
was obtained in the experiment at 20<=>30°C compared to sand, but those differences were not 
statistically significant. When testing was done in germinator with 25°C positive effects of organic 
growing media were identified and this impact was significant, especially for low-quality seed 
lots. The experimental outcome was to continue with routine practice and use organic growing 
media for retesting of seed lots with lower germination as an alternative substrate.
Key words: germination, organic substrate, sand, soybean, temperature, Visoka klijavost semena i uniforman rast klijanaca su presudni za proizvodnju soje, jer od 
toga zavisi gustina useva i na kraju, prinos. Precizna procena potencijala klijavosti setvenog ma terijala je izuzetno važna, kao i početna procena kvaliteta tek požnjevenog semena. Međunarod na pravila za ispitivanje semena propisuju alternativne metode za klijanje semena soje: pesak, 
između filter papira, na filter papiru uz prekrivanje peskom, i od 2020. godine organski supstrat.
U Laboratoriji za ispitivanje semena, Instituta za kukuruz “Zemun Polje”, naklijavanje soje se 
vrši u pesku, na temperaturi 20<=>30°C. Cilj ove studije bio je proceniti nekoliko dostupnih 
organskih supstrata i uporediti dobijene rezultate sa dosadašnjom laboratorijskom metodom.
Pet partija soje različitog kvaliteta testirano je u četiri podloge za naklijavanje (3 organske i pe sak), pri dva temperaturna režima (25°C i 20<=>30°C). Statistička analiza pokazala je da su sve 
organske podloge istog kvaliteta. Nešto veća klijavost u organskom supstratu u poređenju sa pes kom je ostvarena u eksperimentu na 20<=>30°C, ali te razlike nisu bile statistički značajne. Pri likom ispitivanja u klijalištu sa 25°C pokazali su se pozitivni efekti organske podloge i ovaj uticaj 
je bio značajan, posebno za partije slabijeg kvaliteta. Nakon analize eksperimentalnih rezultata 
odlučeno je da se nastavi sa dosadašnjom laboratorijskom praksom naklijavanja u pesku i da se 
organska podloga koristi kao alternativni supstrat za ponovno ispitivanje partija semena sa nižim 
procentom klijavosti.",
publisher = "Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Experimental evaluation of organic substrates for germination testing of soybean seed, Eksperimentalna procena organskih podloga za ispitivanje klijavosti semena soje",
volume = "26",
number = "2",
pages = "1-6",
doi = "10.5937/SelSem2002001M"
}

Milivojević, M., Branković-Radojčić, D. V., Vukadinović, R., Kojić, J., Dumanović, Z.,& Petrović, T.. (2020). Experimental evaluation of organic substrates for germination testing of soybean seed. in Selekcija i semenarstvo
Beograd : Društvo selekcionara i semenara Republike Srbije., 26(2), 1-6.
https://doi.org/10.5937/SelSem2002001M

Milivojević M, Branković-Radojčić DV, Vukadinović R, Kojić J, Dumanović Z, Petrović T. Experimental evaluation of organic substrates for germination testing of soybean seed. in Selekcija i semenarstvo. 2020;26(2):1-6.
doi:10.5937/SelSem2002001M .

Milivojević, Marija, Branković-Radojčić, Dragana V., Vukadinović, Radmila, Kojić, Jasna, Dumanović, Zoran, Petrović, Tanja, "Experimental evaluation of organic substrates for germination testing of soybean seed" in Selekcija i semenarstvo, 26, no. 2 (2020):1-6,
https://doi.org/10.5937/SelSem2002001M . .

TY  - JOUR
AU  - Kojic, Jasna
AU  - Milivojević, Marija
AU  - Petrović, Tanja
AU  - Vukadinović, Radmila
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/971
AB  - U Laboratoriji za ispitivaje semena Instituta za kukuruz Zemun Polje, 
u toku redovnog ispitivanja klijavosti semena suncokreta, uoĉeno je 
kod dve partije semena veliki broj neklijalog semena. Seme je 
stavljeno na naklijavanje dva puta sa razliĉitim temperaturnim 
režimom. U toku prvog ispitivanja seme je stavljeno na klijanje 
metodom između filter papira, hlađenje semena je trajalo 6 dana, 
energija klijanja je oĉitana ĉetvrtog dana a ukupna klijavost desetog 
dana. Seme je naklijavano u klijalištu na 200C sa režmom svetlosti 8 
sati dan, 16 sati noć. U toku drugog ispitivanja, seme suncokreta je 
postavljeno na klijanje istom metodom između filter papira, hlađeno 
je 6 dana, energija klijanja je oĉitana ĉetvrti dan a završna klijavost 
deseti dan. Naklijavanje je obavljeno u sobi klijalištu na naizmeniĉnoj 
temperaturi 20<=>300C, sa svetlosnim režimom 8 sati dan, 16 sati 
noć. Urađen je i TTZ - tetrazolium test. Analizom dobijenih rezultata 
uoĉeno je da na kraju prvog ispitivanja izdvojen veliki broj neklijalog 
semena koje je bilo sveže bez simptoma bolesti. Na kraju drugog 
ispitivanja taj procenat je bio manji u odnosu na prvo ispitivanje. 
Naklijavanje semena na većoj, naizmeniĉnoj temperaturi 20<=>300C, 
u toku drugog ispitivanja, smanjilo je broj neklijalog semena. TTZ 
testom je potvrđena vitalnost neklijalog semena.
AB  - A great number of nongerminated sunflower seed of two lots were 
observed during regular testing in the Seed Testing Laboratory of the 
Maize Research Institute, Zemun Polje. Seeds were germinated 
twice under different temperature regimes. In the course of the first 
test, seeds were germinated between filter paper, cooling lasted 6 
days, the first count was done on the fourth day, while total 
germination was determined on the 10th day. Seeds were germinated 
in the germination cabinet at 200oC with 8 h (light) to16 h (night) 
regime. In the course of the second test, the method applied was the 
same: sunflower seeds were germinated between filter paper, 
cooling lasted 6 days, the first count was done on the fourth day, 
while total germination was determined on the 10th day. Germination 
was performed in the germination cabinet at the alternating 
temperature of 20<=>300oC, and the light regime of 8 h (light) to16 h 
(night). The TTZ -tetrazolium test was also performed. The analysis 
of obtained results showed that a great number of nongermination, 
but fresh and disease-free seeds were detected at the end of the first 
test. The corresponding percentage at the end of the second test 
was smaller. Seed germination at higher, alternating temperatures of 
20<=>300oC, in the course of the second test, reduced the number of 
nongerminated seeds. Seed viability was confirmed by the TTZ test.
PB  - Beograd : Društvo genetiĉara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
T2  - 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata
T1  - Ispitivanje dormantnosti semena suncokreta
T1  - Evaluation of dormance sunflower seed lots
SP  - 223
EP  - 224
UR  - https://hdl.handle.net/21.15107/rcub_rik_971
ER  - 

@article{
author = "Kojic, Jasna and Milivojević, Marija and Petrović, Tanja and Vukadinović, Radmila",
year = "2018",
abstract = "U Laboratoriji za ispitivaje semena Instituta za kukuruz Zemun Polje, 
u toku redovnog ispitivanja klijavosti semena suncokreta, uoĉeno je 
kod dve partije semena veliki broj neklijalog semena. Seme je 
stavljeno na naklijavanje dva puta sa razliĉitim temperaturnim 
režimom. U toku prvog ispitivanja seme je stavljeno na klijanje 
metodom između filter papira, hlađenje semena je trajalo 6 dana, 
energija klijanja je oĉitana ĉetvrtog dana a ukupna klijavost desetog 
dana. Seme je naklijavano u klijalištu na 200C sa režmom svetlosti 8 
sati dan, 16 sati noć. U toku drugog ispitivanja, seme suncokreta je 
postavljeno na klijanje istom metodom između filter papira, hlađeno 
je 6 dana, energija klijanja je oĉitana ĉetvrti dan a završna klijavost 
deseti dan. Naklijavanje je obavljeno u sobi klijalištu na naizmeniĉnoj 
temperaturi 20<=>300C, sa svetlosnim režimom 8 sati dan, 16 sati 
noć. Urađen je i TTZ - tetrazolium test. Analizom dobijenih rezultata 
uoĉeno je da na kraju prvog ispitivanja izdvojen veliki broj neklijalog 
semena koje je bilo sveže bez simptoma bolesti. Na kraju drugog 
ispitivanja taj procenat je bio manji u odnosu na prvo ispitivanje. 
Naklijavanje semena na većoj, naizmeniĉnoj temperaturi 20<=>300C, 
u toku drugog ispitivanja, smanjilo je broj neklijalog semena. TTZ 
testom je potvrđena vitalnost neklijalog semena., A great number of nongerminated sunflower seed of two lots were 
observed during regular testing in the Seed Testing Laboratory of the 
Maize Research Institute, Zemun Polje. Seeds were germinated 
twice under different temperature regimes. In the course of the first 
test, seeds were germinated between filter paper, cooling lasted 6 
days, the first count was done on the fourth day, while total 
germination was determined on the 10th day. Seeds were germinated 
in the germination cabinet at 200oC with 8 h (light) to16 h (night) 
regime. In the course of the second test, the method applied was the 
same: sunflower seeds were germinated between filter paper, 
cooling lasted 6 days, the first count was done on the fourth day, 
while total germination was determined on the 10th day. Germination 
was performed in the germination cabinet at the alternating 
temperature of 20<=>300oC, and the light regime of 8 h (light) to16 h 
(night). The TTZ -tetrazolium test was also performed. The analysis 
of obtained results showed that a great number of nongermination, 
but fresh and disease-free seeds were detected at the end of the first 
test. The corresponding percentage at the end of the second test 
was smaller. Seed germination at higher, alternating temperatures of 
20<=>300oC, in the course of the second test, reduced the number of 
nongerminated seeds. Seed viability was confirmed by the TTZ test.",
publisher = "Beograd : Društvo genetiĉara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata",
title = "Ispitivanje dormantnosti semena suncokreta, Evaluation of dormance sunflower seed lots",
pages = "223-224",
url = "https://hdl.handle.net/21.15107/rcub_rik_971"
}

Kojic, J., Milivojević, M., Petrović, T.,& Vukadinović, R.. (2018). Ispitivanje dormantnosti semena suncokreta. in 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata
Beograd : Društvo genetiĉara Srbije., 223-224.
https://hdl.handle.net/21.15107/rcub_rik_971

Kojic J, Milivojević M, Petrović T, Vukadinović R. Ispitivanje dormantnosti semena suncokreta. in 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata. 2018;:223-224.
https://hdl.handle.net/21.15107/rcub_rik_971 .

Kojic, Jasna, Milivojević, Marija, Petrović, Tanja, Vukadinović, Radmila, "Ispitivanje dormantnosti semena suncokreta" in 6 Simpozijum Sekcije za oplemenjivanje organizama Društva Genetiĉara Srbije i 9 Simpozijum Društva selekcionera i  semenara Republike Srbije, 07-11.05.2018. - Zbornik apstrakata (2018):223-224,
https://hdl.handle.net/21.15107/rcub_rik_971 .

TY  - JOUR
AU  - Milivojević, Marija
AU  - Kojić, Jasna
AU  - Vukadinović, Radmila
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Petrović, Tanja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/784
AB  - Pojava tvrdog semena kao posledica nepropustljivosti semenjače za vodu je izražena kod vrsta iz familije Fabaceae. Kod divljih biljaka ova osobina je biološki opravdana jer omoguća-va bolji opstanak, ali za poljoprivredu tvrda semena su nepoželjna jer klijaju sa zakašnjenjem i neravnomerno. Prilikom ispitivanja klijavosti semena ovih vrsta u laboratoriji može se primeniti predtretman (prethodno hlađenje, mehanička skarifikacija) pre naklijavanja u optimalnim uslovi-ma. U ovom radu su prikazani rezultati ispitivanja klijavosti semena Trifolium repens sprovedena u 150 laboratorija širom sveta u okviru Proficiency testa 17-2. Ovi testovi su organizovani od strane Međunarodne organizacije za ispitivanje semena (ISTA), a obavezni su za sve akredi-tovane laboratorije, jer se na taj način kontroliše kompetentnost osoblja, ispravnost aparata i pou-zdanost primenjenih metoda ispitivanja. U avgustu 2017. godine distribuirani su uzorci tri partije semena Trifolium repens. U Laboratoriji za ispitivanje semena u Institutu za kukuruz Zemun Polje (LIZP), ispitana je klijavost u septembru primenom mehaničke skarifikacije i prethodnog hlađenja (2 dana na 5-10°C), a zatim klijanje na 20°C. Od 90 akreditovanih laboratorija samo je još jedna primenila mehaničku skarifikaciju, 42 laboratorije nisu primenile nikakav predtretman, a 39 laboratorija je primenilo prethodno hlađenje. Dobijeni rezultati klijavosti u LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) su bili iznad proseka svih laboratorija (Lot1-76.95%, Lot2-79.63%, Lot3-83.27%). Kao glavni zaključak ovog Proficiency testa jeste da je skarifikacija se-mena značajno smanjila broj tvrdih semena u korist normalnih klijanaca. Razlog retke primene skarifikacije može biti potreba korisnika da dobije adekvatnu informaciju vezanu za broj tvrdih semena koja će se pojaviti prilikom setve semena.
AB  - The  presence  of  hard  seeds  as  a  result  of  water  impermeable  seed  coat  is  common  in  species  of  Fabaceae  family.  For  wild  plants,  this  characteristic  allows  better  survival,  but  for  agriculture  production,  hard  seeds  are  undesirable,  due  to  delayed  and  uneven  germination.  In  seed testing laboratory several pre-treatments (pre-chill, mechanical scarification) can be applied for germination testing of these species. In this paper, Proficiency test 17-2 germination results of Trifolium repens seeds conducted in 150 laboratories around the world are presented. Those tests are organized by the International Seed Testing Association (ISTA) and are mandatory for all accredited laboratories. In this way laboratory’s competence (staff, apparatus, test methods) is checked. In August 2017, samples of three Trifolium  repens  seed  lots  were  distributed.  In  the Seed Testing Laboratory at the Maize Research Institute Zemun Polje (LIZP), germination was tested in September using mechanical scarification and pre-chilling (2 days at 5-10°C) prior to germination at 20°C. Among 90 accredited participants, only one more laboratory applied mechanical scarification, 42 laboratories did not use any pre-treatment, and 39 laboratories applied pre-chilling. Obtained germination results in LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) were higher than overall mean (Lot1-76.95%, Lot2-79.63%, and Lot3-83.27%). The main conclusion of this Proficiency test is that seed scarification significantly reduced the number of hard seeds in favour of normal seedlings. The reason for the rare application of scarification may be the requirement of the applicant to obtain adequate information regarding the number of hard seeds that will appear during seed sowing.
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2
T1  - ISTA proficiency test 17-2 germination results of white clover seed
VL  - 24
IS  - 1
SP  - 11
EP  - 17
DO  - 10.5937/SelSem1801011M
ER  - 

@article{
author = "Milivojević, Marija and Kojić, Jasna and Vukadinović, Radmila and Stanisavljević, Rade and Poštić, Dobrivoj and Petrović, Tanja",
year = "2018",
abstract = "Pojava tvrdog semena kao posledica nepropustljivosti semenjače za vodu je izražena kod vrsta iz familije Fabaceae. Kod divljih biljaka ova osobina je biološki opravdana jer omoguća-va bolji opstanak, ali za poljoprivredu tvrda semena su nepoželjna jer klijaju sa zakašnjenjem i neravnomerno. Prilikom ispitivanja klijavosti semena ovih vrsta u laboratoriji može se primeniti predtretman (prethodno hlađenje, mehanička skarifikacija) pre naklijavanja u optimalnim uslovi-ma. U ovom radu su prikazani rezultati ispitivanja klijavosti semena Trifolium repens sprovedena u 150 laboratorija širom sveta u okviru Proficiency testa 17-2. Ovi testovi su organizovani od strane Međunarodne organizacije za ispitivanje semena (ISTA), a obavezni su za sve akredi-tovane laboratorije, jer se na taj način kontroliše kompetentnost osoblja, ispravnost aparata i pou-zdanost primenjenih metoda ispitivanja. U avgustu 2017. godine distribuirani su uzorci tri partije semena Trifolium repens. U Laboratoriji za ispitivanje semena u Institutu za kukuruz Zemun Polje (LIZP), ispitana je klijavost u septembru primenom mehaničke skarifikacije i prethodnog hlađenja (2 dana na 5-10°C), a zatim klijanje na 20°C. Od 90 akreditovanih laboratorija samo je još jedna primenila mehaničku skarifikaciju, 42 laboratorije nisu primenile nikakav predtretman, a 39 laboratorija je primenilo prethodno hlađenje. Dobijeni rezultati klijavosti u LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) su bili iznad proseka svih laboratorija (Lot1-76.95%, Lot2-79.63%, Lot3-83.27%). Kao glavni zaključak ovog Proficiency testa jeste da je skarifikacija se-mena značajno smanjila broj tvrdih semena u korist normalnih klijanaca. Razlog retke primene skarifikacije može biti potreba korisnika da dobije adekvatnu informaciju vezanu za broj tvrdih semena koja će se pojaviti prilikom setve semena., The  presence  of  hard  seeds  as  a  result  of  water  impermeable  seed  coat  is  common  in  species  of  Fabaceae  family.  For  wild  plants,  this  characteristic  allows  better  survival,  but  for  agriculture  production,  hard  seeds  are  undesirable,  due  to  delayed  and  uneven  germination.  In  seed testing laboratory several pre-treatments (pre-chill, mechanical scarification) can be applied for germination testing of these species. In this paper, Proficiency test 17-2 germination results of Trifolium repens seeds conducted in 150 laboratories around the world are presented. Those tests are organized by the International Seed Testing Association (ISTA) and are mandatory for all accredited laboratories. In this way laboratory’s competence (staff, apparatus, test methods) is checked. In August 2017, samples of three Trifolium  repens  seed  lots  were  distributed.  In  the Seed Testing Laboratory at the Maize Research Institute Zemun Polje (LIZP), germination was tested in September using mechanical scarification and pre-chilling (2 days at 5-10°C) prior to germination at 20°C. Among 90 accredited participants, only one more laboratory applied mechanical scarification, 42 laboratories did not use any pre-treatment, and 39 laboratories applied pre-chilling. Obtained germination results in LIZP (Lot1-84.50%, Lot2-84%, Lot3-88.50%) were higher than overall mean (Lot1-76.95%, Lot2-79.63%, and Lot3-83.27%). The main conclusion of this Proficiency test is that seed scarification significantly reduced the number of hard seeds in favour of normal seedlings. The reason for the rare application of scarification may be the requirement of the applicant to obtain adequate information regarding the number of hard seeds that will appear during seed sowing.",
publisher = "Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2, ISTA proficiency test 17-2 germination results of white clover seed",
volume = "24",
number = "1",
pages = "11-17",
doi = "10.5937/SelSem1801011M"
}

Milivojević, M., Kojić, J., Vukadinović, R., Stanisavljević, R., Poštić, D.,& Petrović, T.. (2018). Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2. in Selekcija i semenarstvo
Beograd : Društvo selekcionara i semenara Republike Srbije., 24(1), 11-17.
https://doi.org/10.5937/SelSem1801011M

Milivojević M, Kojić J, Vukadinović R, Stanisavljević R, Poštić D, Petrović T. Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2. in Selekcija i semenarstvo. 2018;24(1):11-17.
doi:10.5937/SelSem1801011M .

Milivojević, Marija, Kojić, Jasna, Vukadinović, Radmila, Stanisavljević, Rade, Poštić, Dobrivoj, Petrović, Tanja, "Rezultati klijavosti semena bele deteline u ISTA proficiency testu 17-2" in Selekcija i semenarstvo, 24, no. 1 (2018):11-17,
https://doi.org/10.5937/SelSem1801011M . .

TY  - JOUR
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Branković-Radojčić, Dragana V.
AU  - Vukadinović, Radmila
AU  - Kojić, Jasna
AU  - Petrović, Tanja
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/672
AB  - Cold test (CT) is a valuable method of assessing maize seed vigour. Although widely used CT is not standardized because of variations in CT procedures. The objective of this study was to evaluate seed vigour of 15 maize inbred lines developed at the Maize Research Institute Zemun Polje, using two different substrates (soil and sand) in a severe CT (7.5°C for 10 days followed by 7 days at 20/30 °C). Statistical analysis showed that inbred lines differ significantly in seed vigour, and no difference between two applied substrates in CT. Coefficients of variation were lower in CT with sand (6.05 %), compared to the CT with soil (6.74%) due to variation in soil quality and presence of soil-borne pathogens. Both CT procedures were highly correlated with field emergence. Results of this research indicate that CT with sand is appropriate for testing maize seed vigour, with the potential for standardization.
AB  - Vigor semena je veoma važan parametar kvaliteta, jer ukazuje na sposobnost semena da klija u suboptimalnim uslovima (niska temperatura, visoka vlažnost zemljišta itd.). Hladni test je važan metod za utvrđivanje vigora semena kukuruza. Iako je dobar pokazatelj nicanja u polju, i koristi se širom sveta, hladni test nije standardizovan zbog razlika u proceduri (temperatura, vrsta i vlažnost supstrata). Cilj ovog rada bio je ispitivanje vigora semena samooplodnih linija kukuruza primenom dva različita supstrata (zemlja i pesak) u hladnom testu. Za ispitivanje je odabrano 15 samooplodnih linija kukuruza stvorenih u Institutu za kukuruz 'Zemun Polje'. Primenjeni su izrazito stresni uslovi ispitivanja u hladnom testu (10 dana na 7,5°C, a zatim 7 dana na 20/30°C). Statističkom obradom podataka utvrđeno je da su se samooplodne linije značajno razlikovale po vigoru semena tj. tolerantnosti na niske temperature u periodu klijanja i početnog porasta. Sa druge strane nije utvrđena značajna razlika između dva primenjena supstrata u hladnom testu. Prosečna klijavost 15 samooplodnih linija u hladnom testu sa peskom iznosila je 82,2%, a u hladnom testu sa zemljom 80,1%. Koeficijent varijacije je bio niži u hladnom testu sa peskom (6,05%), nego u hladnom testu sa zemljom (6,74%), što se objašnjava variranjem u kvalitetu zemlje i prisustvu zemljišnih patogena. Obe ispitivane procedure su postigle visoku korelaciju sa nicanjem u polju. Rezultati ovih istraživanja ukazuju na mogućnost korišćenja peska kao supstrata u hladnom testu, čime bi se ispitivanje vigora semena kukuruza znatno olakšalo, a ujedno bi se stekli uslovi za standardizaciju hladnog testa.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines
T1  - Poređenje dve procedure hladnog testa za ispitivanje vigora semena samooplodnih linija kukuruza
VL  - 21
IS  - 2
SP  - 115
EP  - 117
DO  - 10.5937/JPEA1702115M
ER  - 

@article{
author = "Milivojević, Marija and Srdić, Jelena and Branković-Radojčić, Dragana V. and Vukadinović, Radmila and Kojić, Jasna and Petrović, Tanja",
year = "2017",
abstract = "Cold test (CT) is a valuable method of assessing maize seed vigour. Although widely used CT is not standardized because of variations in CT procedures. The objective of this study was to evaluate seed vigour of 15 maize inbred lines developed at the Maize Research Institute Zemun Polje, using two different substrates (soil and sand) in a severe CT (7.5°C for 10 days followed by 7 days at 20/30 °C). Statistical analysis showed that inbred lines differ significantly in seed vigour, and no difference between two applied substrates in CT. Coefficients of variation were lower in CT with sand (6.05 %), compared to the CT with soil (6.74%) due to variation in soil quality and presence of soil-borne pathogens. Both CT procedures were highly correlated with field emergence. Results of this research indicate that CT with sand is appropriate for testing maize seed vigour, with the potential for standardization., Vigor semena je veoma važan parametar kvaliteta, jer ukazuje na sposobnost semena da klija u suboptimalnim uslovima (niska temperatura, visoka vlažnost zemljišta itd.). Hladni test je važan metod za utvrđivanje vigora semena kukuruza. Iako je dobar pokazatelj nicanja u polju, i koristi se širom sveta, hladni test nije standardizovan zbog razlika u proceduri (temperatura, vrsta i vlažnost supstrata). Cilj ovog rada bio je ispitivanje vigora semena samooplodnih linija kukuruza primenom dva različita supstrata (zemlja i pesak) u hladnom testu. Za ispitivanje je odabrano 15 samooplodnih linija kukuruza stvorenih u Institutu za kukuruz 'Zemun Polje'. Primenjeni su izrazito stresni uslovi ispitivanja u hladnom testu (10 dana na 7,5°C, a zatim 7 dana na 20/30°C). Statističkom obradom podataka utvrđeno je da su se samooplodne linije značajno razlikovale po vigoru semena tj. tolerantnosti na niske temperature u periodu klijanja i početnog porasta. Sa druge strane nije utvrđena značajna razlika između dva primenjena supstrata u hladnom testu. Prosečna klijavost 15 samooplodnih linija u hladnom testu sa peskom iznosila je 82,2%, a u hladnom testu sa zemljom 80,1%. Koeficijent varijacije je bio niži u hladnom testu sa peskom (6,05%), nego u hladnom testu sa zemljom (6,74%), što se objašnjava variranjem u kvalitetu zemlje i prisustvu zemljišnih patogena. Obe ispitivane procedure su postigle visoku korelaciju sa nicanjem u polju. Rezultati ovih istraživanja ukazuju na mogućnost korišćenja peska kao supstrata u hladnom testu, čime bi se ispitivanje vigora semena kukuruza znatno olakšalo, a ujedno bi se stekli uslovi za standardizaciju hladnog testa.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines, Poređenje dve procedure hladnog testa za ispitivanje vigora semena samooplodnih linija kukuruza",
volume = "21",
number = "2",
pages = "115-117",
doi = "10.5937/JPEA1702115M"
}

Milivojević, M., Srdić, J., Branković-Radojčić, D. V., Vukadinović, R., Kojić, J.,& Petrović, T.. (2017). Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 21(2), 115-117.
https://doi.org/10.5937/JPEA1702115M

Milivojević M, Srdić J, Branković-Radojčić DV, Vukadinović R, Kojić J, Petrović T. Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines. in Journal on Processing and Energy in Agriculture. 2017;21(2):115-117.
doi:10.5937/JPEA1702115M .

Milivojević, Marija, Srdić, Jelena, Branković-Radojčić, Dragana V., Vukadinović, Radmila, Kojić, Jasna, Petrović, Tanja, "Comparison of two cold test procedures for seed vigour evaluation of maize inbred lines" in Journal on Processing and Energy in Agriculture, 21, no. 2 (2017):115-117,
https://doi.org/10.5937/JPEA1702115M . .

TY  - JOUR
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Stanković, Slavica
AU  - Milivojević, Marija
AU  - Vukadinović, Radmila
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/478
AB  - The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Detection of Rhizomucor pusillus on sunflower seed
VL  - 36
IS  - 59
SP  - 59
EP  - 70
DO  - 10.2298/HEL1359059L
ER  - 

@article{
author = "Lević, Jelena and Ivanović, D. and Stanković, Slavica and Milivojević, Marija and Vukadinović, Radmila and Stepanić, Ana",
year = "2013",
abstract = "The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Detection of Rhizomucor pusillus on sunflower seed",
volume = "36",
number = "59",
pages = "59-70",
doi = "10.2298/HEL1359059L"
}

Lević, J., Ivanović, D., Stanković, S., Milivojević, M., Vukadinović, R.,& Stepanić, A.. (2013). Detection of Rhizomucor pusillus on sunflower seed. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 36(59), 59-70.
https://doi.org/10.2298/HEL1359059L

Lević J, Ivanović D, Stanković S, Milivojević M, Vukadinović R, Stepanić A. Detection of Rhizomucor pusillus on sunflower seed. in Helia. 2013;36(59):59-70.
doi:10.2298/HEL1359059L .

Lević, Jelena, Ivanović, D., Stanković, Slavica, Milivojević, Marija, Vukadinović, Radmila, Stepanić, Ana, "Detection of Rhizomucor pusillus on sunflower seed" in Helia, 36, no. 59 (2013):59-70,
https://doi.org/10.2298/HEL1359059L . .