Prolonged heat stress during winter diapause alters the expression of stress-response genes in Ostrinia nubilalis (Hbn.)
Аутори
Uzelac, IvaAvramov, Miloš
Knežić, Teodora
Tatić, Vanja
Gošić-Dondo, Snežana
Popović, Željko D.
Чланак у часопису (Објављена верзија)
Метаподаци
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During diapause, a state of temporarily arrested development, insects require low winter
temperatures to suppress their metabolism, conserve energy stores and acquire cold hardiness.
A warmer winter could, thus, reduce diapause incidence and duration in many species, prematurely
deplete their energy reserves and compromise post-diapause fitness. In this study, we investigated
the combined effects of thermal stress and the diapause program on the expression of selected genes
involved in antioxidant defense and heat shock response in the European corn borer Ostrinia nubilalis.
By using qRT-PCR, it has been shown that response to chronic heat stress is characterized by raised
mRNA levels of grx and trx, two important genes of the antioxidant defense system, as well as of
hsp70 and, somewhat, of hsp90, two major heat shock response proteins. On the other hand, the
expression of hsc70, hsp20.4 and hsp20.1 was discontinuous in the latter part of diapause, or was
strongly controlled... by the diapause program and refractory to heat stress, as was the case for mtn
and fer, genes encoding two metal storage proteins crucial for metal ion homeostasis. This is the first
time that the effects of high winter temperatures have been assessed on cold-hardy diapausing larvae
and pupae of this important corn pest.
Кључне речи:
diapause / Ostrinia nubilalis / warm acclimation / heat stress / gene expression / heat shock proteins (HSPs) / antioxidative defense system (ADS)Извор:
International Journal of Molecular Sciences, 2024, 25, 3100-Издавач:
- Basel : MDPI
Финансирање / пројекти:
- info:eu-repo/grantAgreement/MESTD/inst- 451-03-66/2024-03/200125/RS// (RS-MESTD-inst- 451-03-66-2024-03-200125)
- nfo:eu-repo/grantAgreement/MESTD/inst- 451-03-65/2024-03/200125/RS//
- info:eu-repo/grantAgreement/MESTD/inst-451-03-66/2024-03/200358// (200358-MESTD-inst-451-03-66-2024-03)