Molecular characterisation of maize hybrids
Authors
Ristić, Danijela
Kostadinović, Marija
Kravić, Natalija

Kovinčić, Anika
Obradović, Ana

Stevanović, Milan

Pavlov, Jovan

Conference object (Published version)
Metadata
Show full item recordAbstract
Despite the huge diversity of maize germplasm, modern maize breeding programme and
agricultural practices decrease the diversity of modern hybrids. Genetic characterization of
maize hybrids allows knowledge of the genetic relationship among them, thus preventing the
risk of increasing uniformity. Because of their high reproducibility, informativeness and easey
application of microsatelites are the most frequently used molecular markers in maize genetic
diversity studies. The aim of our work was to evaluate genetic diversity of maize hybrids by
SSR markers and compare results with their pedigre information. Sixteen polymorphic SSR
(Simple Sequence Repeats) markers were used to characterize 14 maize (Zea mays L.) hybrids
belonging to different breeding programs and FAO groups (from 300 to 800). A total of 53
alleles were found, ranging from two to four alleles. Genetic similarities were calculated in
NTSYSpc2 program package using Jaccard’s coefficient based on binary data (pre...sence or
absence of alleles). The highest value of genetic similarity was 0.80 between H1 and H2,
while the lowest value (0.26) was found between H12 and H13. Cluster analysis was done by
unweighted pair group method (UPGMA) on the basis of genetic similarity matrix.
Dendrogram analysis grouped maize hybrids in one cluster (most of the analyzed genotypes),
one smaller cluster and one branch. The results revealed genetic heterogeneity between analyzed maize hybrids.
Keywords:
maize hybrids / genetic similarity / SSR markers / zea mays lSource:
10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina, 2019, 211-215Publisher:
- Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia