Molecular characterisation of soybean varieties by SSR markers
Authors
Ristic, DanijelaFilipovski, Filip
Perić, Vesna
Mladenović Drinić, Snežana
Kovinčić, Anika
Kravić, Natalija
Kostadinović, Marija
Conference object (Published version)
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Show full item recordAbstract
Soybean (Glycine max (L.) Merr.) is one of the most economically important legumes.
As the source of plant protein and vegetable oil it is used as food and industrial crop in
many regions of the world. The genetic base of soybean cultivars is highly narrow,
corresponding to the fact that it is largely a self-pollinated species. Twelve soybean
varieties were evaluated with SSR (Simple Sequence Repeat) markers selected based on
their distribution on the 20 genetic linkage groups. Out of 36 SSR markers, 33 markers
were found polymorphic among analyzed genotypes. Total number of alleles was 88,
ranging between two and four with an average of 2.67 alleles per marker. The
polymorphic information content (PIC) ranged from 0.153 (Satt229, Satt239 and
Satt327) to 0.775 (Satt276). Simple matching similarity coefficient was calculated using
NTSYSpc2 program package. The average genetic similarity coefficient for all pairwise
was 0.57, with highest value (0.84) between Galina and Lela, ...while the lowest value
(0.46) was found between Bosa and Nena. Dendrogram by the UPGMA (Unweighted
Pair Group Method with Arithmetic Mean) method was constructed on the basis of
genetic similarity matrix. Genotypes were distributed in two groups and one branch,
mostly in accordance with their pedigree.
Keywords:
genetic similarity / SSR / glycine maxSource:
6. congress of the Serbian genetic society, 13-1.10. 2019, Vrnjačka Banja, Serbia - Book of proceedings, 2020, 90-96Publisher:
- Belgrade : Serbian genetic society