Two populations of Amaranthus retroflexus with different morphology were collected from field of the Maize Research Institute Zemun Polje, Serbia. Random amplified polymorphic DNA (RAPD) and seed protein analysis were performed to study the genetic differences in two grain Amaranthus populations. The studied populations have different protein and DNA profile. A total of 171 DNA fragments were generated by 31 RAPD primers, with an average of 5.5 fragments per primer. Of these, 61.4% fragments were polymorphic among the two populations. 18 protein fraction were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The populations differed in the four protein fractions of different molecular weight. The seed protein electrophoresis and RAPD markers are useful for genetic determination of A. retroflexus populations and identification of biotypes with atypical morphology.
TY - JOUR
AU - Mladenović Drinić, Snežana
AU - Kostadinović, Marija
AU - Ristić, Danijela
AU - Simić, Milena
AU - Stefanović, Lidija
PY - 2012
UR - http://rik.mrizp.rs/handle/123456789/968
AB - Two populations of Amaranthus retroflexus with different morphology were collected from field of the Maize Research Institute Zemun Polje, Serbia. Random amplified polymorphic DNA (RAPD) and seed protein analysis were performed to study the genetic differences in two grain Amaranthus populations. The studied populations have different protein and DNA profile. A total of 171 DNA fragments were generated by 31 RAPD primers, with an average of 5.5 fragments per primer. Of these, 61.4% fragments were polymorphic among the two populations. 18 protein fraction were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The populations differed in the four protein fractions of different molecular weight. The seed protein electrophoresis and RAPD markers are useful for genetic determination of A. retroflexus populations and identification of biotypes with atypical morphology.
PB - Nigeria : Academic Journals
T2 - African Journal of Biotechnology
T1 - Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers
VL - 11
IS - 29
SP - 7331
EP - 7337
DO - 10.5897/AJB11.1254
ER -
@article{
author = "Mladenović Drinić, Snežana and Kostadinović, Marija and Ristić, Danijela and Simić, Milena and Stefanović, Lidija",
year = "2012",
abstract = "Two populations of Amaranthus retroflexus with different morphology were collected from field of the Maize Research Institute Zemun Polje, Serbia. Random amplified polymorphic DNA (RAPD) and seed protein analysis were performed to study the genetic differences in two grain Amaranthus populations. The studied populations have different protein and DNA profile. A total of 171 DNA fragments were generated by 31 RAPD primers, with an average of 5.5 fragments per primer. Of these, 61.4% fragments were polymorphic among the two populations. 18 protein fraction were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The populations differed in the four protein fractions of different molecular weight. The seed protein electrophoresis and RAPD markers are useful for genetic determination of A. retroflexus populations and identification of biotypes with atypical morphology.",
publisher = "Nigeria : Academic Journals",
journal = "African Journal of Biotechnology",
title = "Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers",
volume = "11",
number = "29",
pages = "7331-7337",
doi = "10.5897/AJB11.1254"
}
Mladenović Drinić, S., Kostadinović, M., Ristić, D., Simić, M.,& Stefanović, L.. (2012). Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers. in African Journal of Biotechnology
Nigeria : Academic Journals., 11(29), 7331-7337.
https://doi.org/10.5897/AJB11.1254
Mladenović Drinić S, Kostadinović M, Ristić D, Simić M, Stefanović L. Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers. in African Journal of Biotechnology. 2012;11(29):7331-7337.
doi:10.5897/AJB11.1254 .
Mladenović Drinić, Snežana, Kostadinović, Marija, Ristić, Danijela, Simić, Milena, Stefanović, Lidija, "Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers" in African Journal of Biotechnology, 11, no. 29 (2012):7331-7337,
https://doi.org/10.5897/AJB11.1254 . .
