Identification of early decline of seed quality by vigor tests
Authors
Petrović, Tanja
Milivojević, Marija

Branković-Radojčić, Dragana V.

Jovanović, Snežana
Vujinović, Jasna
Vukadinović, Radmila
Stojadinović-Životić, Jasmina
Contributors
Anđelković, Violeta
Srdić, Jelena

Nikolić, Milica

Conference object (Published version)
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Show full item recordAbstract
Seed vigor is the quality parameter which indicates germination under wide range of
environmental conditions. This trait can be identified by standard germination test as so-called
first count germination or germination energy. In addition, several tests for vigor evaluation
were adopted by seed testing associations such as ISTA and AOSA. The main purpose for
developing additional vigor tests is to obtain fast and reliable information on seed vigor which
contributes to decision making in the inventory management. ISTA rules prescribes several
tests for evaluating seed vigor, namely: conductivity test, accelerated ageing (AA) for soybean,
controlled deterioration for Brassica seeds, Radicle emergence (RE) and Tetrazolium (TZ)
vigor test for soybean. Seed testing laboratory of the Maize Research Institute is accredited by
ISTA for RE vigor test, has internally developed cold-test and is capable for application of TZ
vigor test for research purposes. These tests were a...pplied on several maize seeds lots with
declining germination energy and still highly rated final germination. Results of vigor tests
were correlated with the standard germination quality parameters, germination energy and final
count, in order to identify vigor test which provides the most approximate output to the standard
germination test. Correlations were significant for all vigor tests, in average 0.882 and 0.713
for germination first and final count, respectively. The highest compatibility between vigor and
germination tests was identified for cold test with correlation coefficients of 0.952 and 0.839
for germination first and final count, respectively. However, due to duration of cold test (2
weeks), it is not capable of providing fast information. Therefore, other two vigor tests, TZ and
RE, which are shorter and have high correlation with germination energy (0.901 and 0.811,
respectively) and final count (0.713 and 0.728, respectively) can be recommended for this
purpose.
Keywords:
germation / seed vigor / radicle emergence test / tetrazolium vigor test / cold testSource:
25. EUCARPIA Maize and Sorghum Conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts, 2022, 61-61Publisher:
- Belgrade : Maize Research Institute, Zemun Polje