Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study
Authors
Bartolić, DraganaMutavdzic, Dragosav
Carstensen, Jens Michael
Stanković, Slavica

Nikolić, Milica

Krstović, Saša
Radotić, Ksenija
Article (Published version)
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Show full item recordAbstract
Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non‑invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B 1 (AFB 1 ) from the uncontaminated seeds. The AFB 1‑contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB 1‑contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB 1‑contaminated seeds showed a 7.9 to 9.6‑fold increase in the seed
reflectance in the VIS region, and 10....4 and 12.2‑fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non‑invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.
Source:
Scientific Reports, 2022, 12, 4849-Publisher:
- Berlin : Springer Nature
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200040 (Maize Research Institute 'Zemun Polje', Belgrade-Zemun) (RS-200040)
- COST ACTION CA, 16101 EU Horizon 2020 (Grant No. 861915)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200117 (University of Novi Sad, Faculty of Agriculture) (RS-200117)