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dc.creatorMesarović, Jelena
dc.creatorDragičević, Vesna
dc.creatorMladenović-Drinić, Snežana
dc.creatorRistić, Danijela
dc.creatorKravić, Natalija
dc.date.accessioned2019-05-16T12:20:46Z
dc.date.available2019-05-16T12:20:46Z
dc.date.issued2017
dc.identifier.issn0352-5139
dc.identifier.urihttp://rik.mrizp.rs/handle/123456789/678
dc.description.abstractAlong with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.en
dc.publisherSrpsko hemijsko društvo, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31068/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31028/RS//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.subjectHPLC-DADen
dc.subjectmethod validationen
dc.subjectphenolic acidsen
dc.subjectcornen
dc.titleDetermination of free phenolic acids from leaves within different colored maizeen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.volume82
dc.citation.issue1
dc.citation.spage63
dc.citation.epage72
dc.citation.other82(1): 63-72
dc.citation.rankM23
dc.identifier.wos000396581500006
dc.identifier.doi10.2298/JSC160512104M
dc.identifier.scopus2-s2.0-85011660655
dc.identifier.fulltexthttp://rik.mrizp.rs//bitstream/id/2622/676.pdf
dc.identifier.rcubconv_949
dc.type.versionpublishedVersion


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