Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

2016
Authors
Hadži-Tašković-Šukalović, VesnaVučinić, Željko
Vuletić, Mirjana
Marković, Ksenija

Kravić, Natalija

Article (Published version)
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Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roo...ts. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.
Keywords:
nitrate / ammonia / antioxidant enzymes / phenolic compoundsSource:
Spanish Journal of Agricultural Research, 2016, 14, 4Publisher:
- Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
Funding / projects:
DOI: 10.5424/sjar/2016144-8305
ISSN: 1695-971X
WoS: 000389677200002
Scopus: 2-s2.0-85005995778
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MRIZPTY - JOUR AU - Hadži-Tašković-Šukalović, Vesna AU - Vučinić, Željko AU - Vuletić, Mirjana AU - Marković, Ksenija AU - Kravić, Natalija PY - 2016 UR - http://rik.mrizp.rs/handle/123456789/618 AB - Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls. PB - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid T2 - Spanish Journal of Agricultural Research T1 - Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources VL - 14 IS - 4 DO - 10.5424/sjar/2016144-8305 UR - conv_943 ER -
@article{ author = "Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Vuletić, Mirjana and Marković, Ksenija and Kravić, Natalija", year = "2016", abstract = "Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.", publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid", journal = "Spanish Journal of Agricultural Research", title = "Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources", volume = "14", number = "4", doi = "10.5424/sjar/2016144-8305", url = "conv_943" }
Hadži-Tašković-Šukalović, V., Vučinić, Ž., Vuletić, M., Marković, K.,& Kravić, N.. (2016). Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources. in Spanish Journal of Agricultural Research Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 14(4). https://doi.org/10.5424/sjar/2016144-8305 conv_943
Hadži-Tašković-Šukalović V, Vučinić Ž, Vuletić M, Marković K, Kravić N. Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources. in Spanish Journal of Agricultural Research. 2016;14(4). doi:10.5424/sjar/2016144-8305 conv_943 .
Hadži-Tašković-Šukalović, Vesna, Vučinić, Željko, Vuletić, Mirjana, Marković, Ksenija, Kravić, Natalija, "Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources" in Spanish Journal of Agricultural Research, 14, no. 4 (2016), https://doi.org/10.5424/sjar/2016144-8305 ., conv_943 .