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A simple SSR analysis for genetic diversity estimation of maize landraces

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2015
565.pdf (151.5Kb)
Authors
Ignjatović-Micić, Dragana
Ristić, Danijela
Babić, Vojka
Anđelković, Violeta
Vančetović, Jelena
Article (Published version)
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Abstract
A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes,... as genetic distances between flint landraces covered a larger range of values (0.11-0.38) than between dent (0.22 - 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.

Keywords:
accessions / genetic diversity / phenotype / SSR / Zea mays L.
Source:
Genetika, 2015, 47, 1, 53-62
Publisher:
  • Društvo genetičara Srbije, Beograd
Funding / projects:
  • Exploitation of maize diversity to improve grain quality and drought tolerance (RS-31028)

DOI: 10.2298/GENSR1501053I

ISSN: 0534-0012

WoS: 000355248900006

Scopus: 2-s2.0-84929377228
[ Google Scholar ]
11
7
URI
http://rik.mrizp.rs/handle/123456789/567
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Vančetović, Jelena
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/567
AB  - A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11-0.38) than between dent (0.22 - 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - A simple SSR analysis for genetic diversity estimation of maize landraces
VL  - 47
IS  - 1
SP  - 53
EP  - 62
DO  - 10.2298/GENSR1501053I
UR  - conv_905
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Ristić, Danijela and Babić, Vojka and Anđelković, Violeta and Vančetović, Jelena",
year = "2015",
abstract = "A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11-0.38) than between dent (0.22 - 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "A simple SSR analysis for genetic diversity estimation of maize landraces",
volume = "47",
number = "1",
pages = "53-62",
doi = "10.2298/GENSR1501053I",
url = "conv_905"
}
Ignjatović-Micić, D., Ristić, D., Babić, V., Anđelković, V.,& Vančetović, J.. (2015). A simple SSR analysis for genetic diversity estimation of maize landraces. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 53-62.
https://doi.org/10.2298/GENSR1501053I
conv_905
Ignjatović-Micić D, Ristić D, Babić V, Anđelković V, Vančetović J. A simple SSR analysis for genetic diversity estimation of maize landraces. in Genetika. 2015;47(1):53-62.
doi:10.2298/GENSR1501053I
conv_905 .
Ignjatović-Micić, Dragana, Ristić, Danijela, Babić, Vojka, Anđelković, Violeta, Vančetović, Jelena, "A simple SSR analysis for genetic diversity estimation of maize landraces" in Genetika, 47, no. 1 (2015):53-62,
https://doi.org/10.2298/GENSR1501053I .,
conv_905 .

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