RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions
|dc.description.abstract||The genebank in Maize Research Institute "Zemun Polje" maintains the collection of 2178 local populations of maize, characterised and classified using morphological markers. In this work 13 local populations, some of which were suspected to be duplicate accessions, were subjected to RAPD analysis with OPB 1-20 primers, as well as to RFLP analysis with 30 probes in combination with three restriction enzymes. Data thus obtained were used for calculation of Nei's genetic distances among populations and the resulting distance matrix was used for cluster analysis by UPGMA method. Genetic distances of analysed populations ranged between 0.1311 and 0.5075. Genetic distances calculated from RAPD and RFLP data were highly similar, leading to the conclusion that both markers revealed sufficient amount of genetic polymorphism among populations. Both methods showed a high degree of discrimination between populations, so they can be successfully used for polymorphism validation, genetic distance estimation among maize populations and screening of suspected duplicates in maize gene bank.||en|
|dc.subject||S -- Cluster analysis||en|
|dc.subject||Maize (Zea mays L.)||en|
|dc.title||RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions||en|
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