RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YuSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YuSSSu populations. Additional four RFLPs were identified in DS7u population.
RFLP lokusi, potencijalni indikatori povećanog sadržaja ulja u zrnu, identifikovani su analizom grupnih uzoraka (Bulk Segregant Analysis - BSA) C0 i C9 ciklusa selekcije dve domaće sintetičke populacije kukuruza - DS7u i YuSSSu. Sadržaj ulja u zrnu biljaka ispitivanih ciklusa selekcije obe populacije utvrđen je NMR spektroskopijom. Analiza genoma grupnih uzoraka početnog (C0) i krajnjeg (C9) ciklusa selekcije DS7u i YuSSSu urađena je pomoću 57 RFLP markera UMC core set. Na osnovu promena u frekvenciji alela u grupnim uzorcima u toku procesa selekcije analiziranih populacija utvrđene su informativne probe - probe potencijalno blisko vezane za QTL uključene u kontrolu povećanog sadržaja ulja u zrnu kukuruza. Identifikovana su četiri zajednička RFLP lokusa u obe analizirane populacije, s tim što je u populaciji DS7u identifikovano još četiri RFLP lokusa potencijalnih indikatora povećanog sadržaja ulja u zrnu kukuruza.
TY - JOUR
AU - Marković, Ksenija
AU - Ignjatović-Micić, Dragana
AU - Saratlić, Goran
AU - Lazić-Jančić, Vesna
PY - 2007
UR - http://rik.mrizp.rs/handle/123456789/168
AB - RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YuSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YuSSSu populations. Additional four RFLPs were identified in DS7u population.
AB - RFLP lokusi, potencijalni indikatori povećanog sadržaja ulja u zrnu, identifikovani su analizom grupnih uzoraka (Bulk Segregant Analysis - BSA) C0 i C9 ciklusa selekcije dve domaće sintetičke populacije kukuruza - DS7u i YuSSSu. Sadržaj ulja u zrnu biljaka ispitivanih ciklusa selekcije obe populacije utvrđen je NMR spektroskopijom. Analiza genoma grupnih uzoraka početnog (C0) i krajnjeg (C9) ciklusa selekcije DS7u i YuSSSu urađena je pomoću 57 RFLP markera UMC core set. Na osnovu promena u frekvenciji alela u grupnim uzorcima u toku procesa selekcije analiziranih populacija utvrđene su informativne probe - probe potencijalno blisko vezane za QTL uključene u kontrolu povećanog sadržaja ulja u zrnu kukuruza. Identifikovana su četiri zajednička RFLP lokusa u obe analizirane populacije, s tim što je u populaciji DS7u identifikovano još četiri RFLP lokusa potencijalnih indikatora povećanog sadržaja ulja u zrnu kukuruza.
PB - Savez poljoprivrednih inženjera i tehničara, Beograd
T2 - Journal of Scientific Agricultural Research
T1 - The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations
T1 - Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.)
VL - 68
IS - 2
SP - 5
EP - 15
UR - https://hdl.handle.net/21.15107/rcub_rik_168
ER -
@article{
author = "Marković, Ksenija and Ignjatović-Micić, Dragana and Saratlić, Goran and Lazić-Jančić, Vesna",
year = "2007",
abstract = "RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YuSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YuSSSu populations. Additional four RFLPs were identified in DS7u population., RFLP lokusi, potencijalni indikatori povećanog sadržaja ulja u zrnu, identifikovani su analizom grupnih uzoraka (Bulk Segregant Analysis - BSA) C0 i C9 ciklusa selekcije dve domaće sintetičke populacije kukuruza - DS7u i YuSSSu. Sadržaj ulja u zrnu biljaka ispitivanih ciklusa selekcije obe populacije utvrđen je NMR spektroskopijom. Analiza genoma grupnih uzoraka početnog (C0) i krajnjeg (C9) ciklusa selekcije DS7u i YuSSSu urađena je pomoću 57 RFLP markera UMC core set. Na osnovu promena u frekvenciji alela u grupnim uzorcima u toku procesa selekcije analiziranih populacija utvrđene su informativne probe - probe potencijalno blisko vezane za QTL uključene u kontrolu povećanog sadržaja ulja u zrnu kukuruza. Identifikovana su četiri zajednička RFLP lokusa u obe analizirane populacije, s tim što je u populaciji DS7u identifikovano još četiri RFLP lokusa potencijalnih indikatora povećanog sadržaja ulja u zrnu kukuruza.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations, Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.)",
volume = "68",
number = "2",
pages = "5-15",
url = "https://hdl.handle.net/21.15107/rcub_rik_168"
}
Marković, K., Ignjatović-Micić, D., Saratlić, G.,& Lazić-Jančić, V.. (2007). The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations. in Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 68(2), 5-15.
https://hdl.handle.net/21.15107/rcub_rik_168
Marković K, Ignjatović-Micić D, Saratlić G, Lazić-Jančić V. The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations. in Journal of Scientific Agricultural Research. 2007;68(2):5-15.
https://hdl.handle.net/21.15107/rcub_rik_168 .
Marković, Ksenija, Ignjatović-Micić, Dragana, Saratlić, Goran, Lazić-Jančić, Vesna, "The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations" in Journal of Scientific Agricultural Research, 68, no. 2 (2007):5-15,
https://hdl.handle.net/21.15107/rcub_rik_168 .
