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Molekularna genetika - postepeno uvođenje u oplemenjivanje kukuruza

dc.creatorKonstantinov, Kosana
dc.creatorMladenović Drinić, Snežana
dc.date.accessioned2019-05-16T12:07:12Z
dc.date.available2019-05-16T12:07:12Z
dc.date.issued2007
dc.identifier.issn0534-0012
dc.identifier.urihttp://rik.mrizp.rs/handle/123456789/150
dc.description.abstractEfficiency in plant breeding is determined primarily by the ability to screen for genetic polymorphism, productivity and yield stability early in program. Dependent on the knowledge about the biochemical bases of the trait and nature of its genetic control, trait could be modified either through mutagenesis of genes controlling it or through the transfer of already existing mutant genes, controlling desired trait to different plant genotypes by classic crossing. Objective of this report is to present partly results on the investigation of the possibilities to apply ionizing radiations (fast neutrons, γ -rays) and chemical mutagens (EI, iPMS, EMS, ENU) to get maize and wheat mutants with increased amount and improved protein quality. Besides this approach in mutation breeding, results on the very early investigation of biochemical background of opaque -2 mutation including use of coupled cell - free RNA and protein synthesis containing components from both wild and opaque - 2 maize genotypes (chromatin, RNA polymerase, microsomall fraction, protein bodies) will be presented. Partial results on opaque - 2 gene incorporation in different genetic background are reviewed. Part of report is dealing with different classes of molecular markers (proteins, RFLP, AFLP, RAPD, and SSR) application in maize genome polymorphism investigation. Besides application of different molecular markers classes in the investigation of heterosis phenomena they are useful in biochemical pathway of important traits control determination as well.en
dc.description.abstractEfikasnost procesa oplemenjivanja je primarno determinisana mogućnostima utvrđivanja genetičkog polimorfizma, produktivnosti i stabilnosti prinosa početnog materijala. Poznavanjem biohemijske osnove genetičke kontrole željene osobine moguće je izvršiti poželjnu promenu ili modifikaciju ili indukovanjem mutacija ili unošenjem već postojećih poznatih gena nosioca mutacija klasičnim procesom ukrštanja sa različitim genotipovima. U radu je dat parcijalni pregled indukovanja mutacija kod pšenice i kukuruza, dobijenih korišćenjem različitih vrsta jonizujućeg zračenja (brzi neutroni, γ -zraci) ili hemijskih mutagena (EI, iPMS, EMS, ENU). U drugom delu su prikazani neki od rezultata unošenja opaque-2 gena u različite genetičke osnove kukuruza primenom klasičnih metoda oplemenjivanja. Istovremeno su navedeni rezultati istraživanja biohemijske osnove opaque2 mutacije, uključujući korišćenje ukopčanog bezćelijskog sistema za sintezu RNK i proteina u prisustvu različitih ćelijskih komponenata (hromatin, RNK polimeraza, mikrozomalna frakcija, proteinska telašca) izolovanih iz endosperma normalnog genotipa i opaque-2 mutanta kukuruza. Deo rada predstavlja pregled rezultata istraživanja u oblasti molekularnog oplemenjivanja - korišćenja različitih klasa molekularnih markera (RFLP, AFLP, RAPD, SSR) u izučavanju genetičkog polimorfizma kukuruza. Dat je i prikaz rezultata ispitivanja molekularne osnove heterozisa kod kukuruza korišćenjem molekularnih markera.sr
dc.publisherDruštvo genetičara Srbije, Beograd
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceGenetika
dc.subjectmaizeen
dc.subjectmutagensen
dc.subjectchromatinen
dc.subjectproteinsen
dc.subjectmolecular markersen
dc.subjectheterosisen
dc.titleMolecular genetics: Step by step implementation in maize breedingen
dc.titleMolekularna genetika - postepeno uvođenje u oplemenjivanje kukuruzasr
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.volume39
dc.citation.issue2
dc.citation.spage139
dc.citation.epage154
dc.citation.other39(2): 139-154
dc.identifier.doi10.2298/GENSR0702139K
dc.identifier.fulltexthttp://rik.mrizp.rs//bitstream/id/2091/148.pdf
dc.type.versionpublishedVersion


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