Changes in gene expression in maize kernel in response to water and salt stress
Abstract
Increasing pressure on limited water resources for agriculture, together with the global temperature increase, highlight the importance of breeding for drought-tolerant cultivars. A better understanding of the molecular nature of drought stress can be expected through the use of genomics approaches. Here, a macroarray of approximate to 2500 maize cDNAs was used for determining transcript changes during water- and salt-stress treatments of developing kernels at 15 days after pollination. Normalization of relative transcript abundances was carried out using a human nebulin control sequence. The proportions of transcripts that changed significantly in abundance upon treatment (> 2-fold compared to the control) were determined; 1.5% of the sequences examined were up-regulated by high salinity and 1% by water stress. Both stresses induced 0.8% of the sequences. These include genes involved in various stress responses: abiotic, wounding and pathogen attack (abscisic acid response binding fac...tor, glycine and proline-rich proteins, pathogenesis-related proteins, etc.). The proportion of down-regulated genes was higher than that for up-regulated genes for water stress (3.2%) and lower for salt stress (0.7%), although only eight genes, predominantly involved in energy generation, were down-regulated in both stress conditions. Co-expression of genes of unknown function under defined conditions may help in elucidating their roles in coordinating stress responses.
Keywords:
expression analysis / macroarray / salt and water stress / Zea mays LSource:
Plant Cell Reports, 2006, 25, 1, 71-79Publisher:
- Springer, New York
Funding / projects:
- EU-FP5 project QLK-2000-00302, SeedDesign.
DOI: 10.1007/s00299-005-0037-x
ISSN: 0721-7714
PubMed: 16362303
WoS: 000234875700009
Scopus: 2-s2.0-31344441718
Collections
Institution/Community
MRIZPTY - JOUR AU - Anđelković, Violeta AU - Thompson, R PY - 2006 UR - http://rik.mrizp.rs/handle/123456789/144 AB - Increasing pressure on limited water resources for agriculture, together with the global temperature increase, highlight the importance of breeding for drought-tolerant cultivars. A better understanding of the molecular nature of drought stress can be expected through the use of genomics approaches. Here, a macroarray of approximate to 2500 maize cDNAs was used for determining transcript changes during water- and salt-stress treatments of developing kernels at 15 days after pollination. Normalization of relative transcript abundances was carried out using a human nebulin control sequence. The proportions of transcripts that changed significantly in abundance upon treatment (> 2-fold compared to the control) were determined; 1.5% of the sequences examined were up-regulated by high salinity and 1% by water stress. Both stresses induced 0.8% of the sequences. These include genes involved in various stress responses: abiotic, wounding and pathogen attack (abscisic acid response binding factor, glycine and proline-rich proteins, pathogenesis-related proteins, etc.). The proportion of down-regulated genes was higher than that for up-regulated genes for water stress (3.2%) and lower for salt stress (0.7%), although only eight genes, predominantly involved in energy generation, were down-regulated in both stress conditions. Co-expression of genes of unknown function under defined conditions may help in elucidating their roles in coordinating stress responses. PB - Springer, New York T2 - Plant Cell Reports T1 - Changes in gene expression in maize kernel in response to water and salt stress VL - 25 IS - 1 SP - 71 EP - 79 DO - 10.1007/s00299-005-0037-x ER -
@article{ author = "Anđelković, Violeta and Thompson, R", year = "2006", abstract = "Increasing pressure on limited water resources for agriculture, together with the global temperature increase, highlight the importance of breeding for drought-tolerant cultivars. A better understanding of the molecular nature of drought stress can be expected through the use of genomics approaches. Here, a macroarray of approximate to 2500 maize cDNAs was used for determining transcript changes during water- and salt-stress treatments of developing kernels at 15 days after pollination. Normalization of relative transcript abundances was carried out using a human nebulin control sequence. The proportions of transcripts that changed significantly in abundance upon treatment (> 2-fold compared to the control) were determined; 1.5% of the sequences examined were up-regulated by high salinity and 1% by water stress. Both stresses induced 0.8% of the sequences. These include genes involved in various stress responses: abiotic, wounding and pathogen attack (abscisic acid response binding factor, glycine and proline-rich proteins, pathogenesis-related proteins, etc.). The proportion of down-regulated genes was higher than that for up-regulated genes for water stress (3.2%) and lower for salt stress (0.7%), although only eight genes, predominantly involved in energy generation, were down-regulated in both stress conditions. Co-expression of genes of unknown function under defined conditions may help in elucidating their roles in coordinating stress responses.", publisher = "Springer, New York", journal = "Plant Cell Reports", title = "Changes in gene expression in maize kernel in response to water and salt stress", volume = "25", number = "1", pages = "71-79", doi = "10.1007/s00299-005-0037-x" }
Anđelković, V.,& Thompson, R.. (2006). Changes in gene expression in maize kernel in response to water and salt stress. in Plant Cell Reports Springer, New York., 25(1), 71-79. https://doi.org/10.1007/s00299-005-0037-x
Anđelković V, Thompson R. Changes in gene expression in maize kernel in response to water and salt stress. in Plant Cell Reports. 2006;25(1):71-79. doi:10.1007/s00299-005-0037-x .
Anđelković, Violeta, Thompson, R, "Changes in gene expression in maize kernel in response to water and salt stress" in Plant Cell Reports, 25, no. 1 (2006):71-79, https://doi.org/10.1007/s00299-005-0037-x . .