RIK - Repository of the Maize Research Institute "Zemun Polje"
Maize Research Institute "Zemun Polje", Belgrade, Serbia
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   RIK
  • MRIZP
  • Радови истраживача / Researchers' publications
  • View Item
  •   RIK
  • MRIZP
  • Радови истраживача / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Evaluation of genetic diversity in soybean genotypes with RAPD markers

Procena genetičke divergentnosti genotipova soje pomoću RAPD markera

Thumbnail
2006
140.pdf (158.2Kb)
Authors
Perić, Vesna
Erić, Iva
Mladenović Drinić, Snežana
Srebrić, Mirjana
Article (Published version)
Metadata
Show full item record
Abstract
The choice of parental mating pairs is of a crucial importance in the breeding process. Obtaining a superior progeny can be expected if the parental lines, used for hybridization, are genetically different and possess as much as possible independent loci for a desirable trait, with alleles different in each parent. The utilization of genetic markers can provide the previous information on the genetic similarity of parents, and based on it, the performance of traits in the progeny can be predicted. The objective of this study was to estimate a genetic distance between the genotype ZPS 015 and genotypes L 91-3103, Ravnica, Shine, L 90-14 and ZPS 107. The genotype ZPS 015 was used as a parent in crosses toother five genotypes. All genotypes have good agronomic traits, so it was supposed that crosses among them could result in a superior progeny. Genomic DNA of these six genotypes was amplified with 18 RAPD primers. The results obtained (presence or absence of the DNA band) were transforme...d into a binary system, and were used to calculate Jaccard's similarity coefficient and a simple matching coefficient. The level of polymorphism detected (20%) and genetic distances calculated suggest a small genetic distance between parental pairs, which is in accordance with previous analyses of soybean genome polymorphism. The shortest, i.e. greatest genetic distance, by both coefficients, was determined between genotypes ZPS 015 and L 90-14, i.e. ZPS 015 and ZPS 107, respectively. The data on grain yield of the F4 progeny by each cross, obtained in the two-year trial, showed a negative and non significant correlation with the genetic distance of parents.

Izbor roditeljskih parova jedan je od ključnih momenata u procesu oplemenjivanja. Dobijanje superiornog potomstva može se očekivati ukoliko se za ukrštanje koriste genetički udaljeni roditelji koji sadrže što veći broj nezavisnih lokusa za svojstvo na koje se vrši oplemenjivanje, sa alelima različitim kod oba roditelja. Primenom genetičkih markera moguće je doći do prethodne informacije o genetičkoj sličnosti roditelja i na osnovu nje pretpostaviti osobine potomstva. U ovom radu cilj je bio ispitati genetičku distancu između genotipa ZPS 015 i genotipova L 91-3103, Ravnica, Shine, L 90-14 i ZPS 107. Genotip ZPS 015 korišćen je kao roditelj u ukrštanjima sa navedenih pet genotipova. Svi genotipovi se odlikuju dobrim agronomskim svojstvima, te je pretpostavka da će se njihovim ukrštanjem dobiti superiorno potomstvo. Genomska DNK ovih šest genotipova amplifikovana je pomoću 18 RAPD prajmera. Dobijeni rezultati (prisustvo ili odsustvo trake, tj. DNK fragmenta) prevedeni su u binarne podatk...e i izračunati su koeficijenti sličnosti po Jaccard-u kao i simple matching coefficient. Dobijeni nivo polimorfizma od 20% i izračunate genetičke distance ukazuju na malu genetičku udaljenost između roditeljskih parova, što je u skladu sa dosadašnjim analizama polimorfnosti genoma soje. Najmanja genetička distanca utvrđena je između genotipova ZPS 015 i L 90-14, a najveća između ZPS 015 i L 107, na osnovu oba koeficijenta sličnosti. Prinosi potomstva F4 generacije pet kombinacija ukrštanja, dobijeni na osnovu dvogodišnjih ogleda, nalaze se u nesignifikantnoj i negativnoj korelaciji sa genetičkom udaljenošću roditelja.

Keywords:
genetička divergentnost / RAPD markeri / soja
Source:
Journal of Scientific Agricultural Research, 2006, 67, 1, 33-39
Publisher:
  • Savez poljoprivrednih inženjera i tehničara, Beograd

ISSN: 0354-5695

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_rik_142
URI
http://rik.mrizp.rs/handle/123456789/142
Collections
  • Радови истраживача / Researchers' publications
Institution/Community
MRIZP
TY  - JOUR
AU  - Perić, Vesna
AU  - Erić, Iva
AU  - Mladenović Drinić, Snežana
AU  - Srebrić, Mirjana
PY  - 2006
UR  - http://rik.mrizp.rs/handle/123456789/142
AB  - The choice of parental mating pairs is of a crucial importance in the breeding process. Obtaining a superior progeny can be expected if the parental lines, used for hybridization, are genetically different and possess as much as possible independent loci for a desirable trait, with alleles different in each parent. The utilization of genetic markers can provide the previous information on the genetic similarity of parents, and based on it, the performance of traits in the progeny can be predicted. The objective of this study was to estimate a genetic distance between the genotype ZPS 015 and genotypes L 91-3103, Ravnica, Shine, L 90-14 and ZPS 107. The genotype ZPS 015 was used as a parent in crosses toother five genotypes. All genotypes have good agronomic traits, so it was supposed that crosses among them could result in a superior progeny. Genomic DNA of these six genotypes was amplified with 18 RAPD primers. The results obtained (presence or absence of the DNA band) were transformed into a binary system, and were used to calculate Jaccard's similarity coefficient and a simple matching coefficient. The level of polymorphism detected (20%) and genetic distances calculated suggest a small genetic distance between parental pairs, which is in accordance with previous analyses of soybean genome polymorphism. The shortest, i.e. greatest genetic distance, by both coefficients, was determined between genotypes ZPS 015 and L 90-14, i.e. ZPS 015 and ZPS 107, respectively. The data on grain yield of the F4 progeny by each cross, obtained in the two-year trial, showed a negative and non significant correlation with the genetic distance of parents.
AB  - Izbor roditeljskih parova jedan je od ključnih momenata u procesu oplemenjivanja. Dobijanje superiornog potomstva može se očekivati ukoliko se za ukrštanje koriste genetički udaljeni roditelji koji sadrže što veći broj nezavisnih lokusa za svojstvo na koje se vrši oplemenjivanje, sa alelima različitim kod oba roditelja. Primenom genetičkih markera moguće je doći do prethodne informacije o genetičkoj sličnosti roditelja i na osnovu nje pretpostaviti osobine potomstva. U ovom radu cilj je bio ispitati genetičku distancu između genotipa ZPS 015 i genotipova L 91-3103, Ravnica, Shine, L 90-14 i ZPS 107. Genotip ZPS 015 korišćen je kao roditelj u ukrštanjima sa navedenih pet genotipova. Svi genotipovi se odlikuju dobrim agronomskim svojstvima, te je pretpostavka da će se njihovim ukrštanjem dobiti superiorno potomstvo. Genomska DNK ovih šest genotipova amplifikovana je pomoću 18 RAPD prajmera. Dobijeni rezultati (prisustvo ili odsustvo trake, tj. DNK fragmenta) prevedeni su u binarne podatke i izračunati su koeficijenti sličnosti po Jaccard-u kao i simple matching coefficient. Dobijeni nivo polimorfizma od 20% i izračunate genetičke distance ukazuju na malu genetičku udaljenost između roditeljskih parova, što je u skladu sa dosadašnjim analizama polimorfnosti genoma soje. Najmanja genetička distanca utvrđena je između genotipova ZPS 015 i L 90-14, a najveća između ZPS 015 i L 107, na osnovu oba koeficijenta sličnosti. Prinosi potomstva F4 generacije pet kombinacija ukrštanja, dobijeni na osnovu dvogodišnjih ogleda, nalaze se u nesignifikantnoj i negativnoj korelaciji sa genetičkom udaljenošću roditelja.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Evaluation of genetic diversity in soybean genotypes with RAPD markers
T1  - Procena genetičke divergentnosti genotipova soje pomoću RAPD markera
VL  - 67
IS  - 1
SP  - 33
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_rik_142
ER  - 
@article{
author = "Perić, Vesna and Erić, Iva and Mladenović Drinić, Snežana and Srebrić, Mirjana",
year = "2006",
abstract = "The choice of parental mating pairs is of a crucial importance in the breeding process. Obtaining a superior progeny can be expected if the parental lines, used for hybridization, are genetically different and possess as much as possible independent loci for a desirable trait, with alleles different in each parent. The utilization of genetic markers can provide the previous information on the genetic similarity of parents, and based on it, the performance of traits in the progeny can be predicted. The objective of this study was to estimate a genetic distance between the genotype ZPS 015 and genotypes L 91-3103, Ravnica, Shine, L 90-14 and ZPS 107. The genotype ZPS 015 was used as a parent in crosses toother five genotypes. All genotypes have good agronomic traits, so it was supposed that crosses among them could result in a superior progeny. Genomic DNA of these six genotypes was amplified with 18 RAPD primers. The results obtained (presence or absence of the DNA band) were transformed into a binary system, and were used to calculate Jaccard's similarity coefficient and a simple matching coefficient. The level of polymorphism detected (20%) and genetic distances calculated suggest a small genetic distance between parental pairs, which is in accordance with previous analyses of soybean genome polymorphism. The shortest, i.e. greatest genetic distance, by both coefficients, was determined between genotypes ZPS 015 and L 90-14, i.e. ZPS 015 and ZPS 107, respectively. The data on grain yield of the F4 progeny by each cross, obtained in the two-year trial, showed a negative and non significant correlation with the genetic distance of parents., Izbor roditeljskih parova jedan je od ključnih momenata u procesu oplemenjivanja. Dobijanje superiornog potomstva može se očekivati ukoliko se za ukrštanje koriste genetički udaljeni roditelji koji sadrže što veći broj nezavisnih lokusa za svojstvo na koje se vrši oplemenjivanje, sa alelima različitim kod oba roditelja. Primenom genetičkih markera moguće je doći do prethodne informacije o genetičkoj sličnosti roditelja i na osnovu nje pretpostaviti osobine potomstva. U ovom radu cilj je bio ispitati genetičku distancu između genotipa ZPS 015 i genotipova L 91-3103, Ravnica, Shine, L 90-14 i ZPS 107. Genotip ZPS 015 korišćen je kao roditelj u ukrštanjima sa navedenih pet genotipova. Svi genotipovi se odlikuju dobrim agronomskim svojstvima, te je pretpostavka da će se njihovim ukrštanjem dobiti superiorno potomstvo. Genomska DNK ovih šest genotipova amplifikovana je pomoću 18 RAPD prajmera. Dobijeni rezultati (prisustvo ili odsustvo trake, tj. DNK fragmenta) prevedeni su u binarne podatke i izračunati su koeficijenti sličnosti po Jaccard-u kao i simple matching coefficient. Dobijeni nivo polimorfizma od 20% i izračunate genetičke distance ukazuju na malu genetičku udaljenost između roditeljskih parova, što je u skladu sa dosadašnjim analizama polimorfnosti genoma soje. Najmanja genetička distanca utvrđena je između genotipova ZPS 015 i L 90-14, a najveća između ZPS 015 i L 107, na osnovu oba koeficijenta sličnosti. Prinosi potomstva F4 generacije pet kombinacija ukrštanja, dobijeni na osnovu dvogodišnjih ogleda, nalaze se u nesignifikantnoj i negativnoj korelaciji sa genetičkom udaljenošću roditelja.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Evaluation of genetic diversity in soybean genotypes with RAPD markers, Procena genetičke divergentnosti genotipova soje pomoću RAPD markera",
volume = "67",
number = "1",
pages = "33-39",
url = "https://hdl.handle.net/21.15107/rcub_rik_142"
}
Perić, V., Erić, I., Mladenović Drinić, S.,& Srebrić, M.. (2006). Evaluation of genetic diversity in soybean genotypes with RAPD markers. in Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 67(1), 33-39.
https://hdl.handle.net/21.15107/rcub_rik_142
Perić V, Erić I, Mladenović Drinić S, Srebrić M. Evaluation of genetic diversity in soybean genotypes with RAPD markers. in Journal of Scientific Agricultural Research. 2006;67(1):33-39.
https://hdl.handle.net/21.15107/rcub_rik_142 .
Perić, Vesna, Erić, Iva, Mladenović Drinić, Snežana, Srebrić, Mirjana, "Evaluation of genetic diversity in soybean genotypes with RAPD markers" in Journal of Scientific Agricultural Research, 67, no. 1 (2006):33-39,
https://hdl.handle.net/21.15107/rcub_rik_142 .

DSpace software copyright © 2002-2015  DuraSpace
About RIK - Repository of the Maize Research Institute Zemun Polje | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About RIK - Repository of the Maize Research Institute Zemun Polje | Send Feedback

OpenAIRERCUB