Prolonged heat stress during winter diapause alters the expression of stress-response genes in Ostrinia nubilalis (Hbn.)
Аутори
Uzelac, IvaAvramov, Miloš
Knežić, Teodora
Tatić, Vanja
Gošić-Dondo, Snežana
Popović, Željko D.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
During diapause, a state of temporarily arrested development, insects require low winter
temperatures to suppress their metabolism, conserve energy stores and acquire cold hardiness.
A warmer winter could, thus, reduce diapause incidence and duration in many species, prematurely
deplete their energy reserves and compromise post-diapause fitness. In this study, we investigated
the combined effects of thermal stress and the diapause program on the expression of selected genes
involved in antioxidant defense and heat shock response in the European corn borer Ostrinia nubilalis.
By using qRT-PCR, it has been shown that response to chronic heat stress is characterized by raised
mRNA levels of grx and trx, two important genes of the antioxidant defense system, as well as of
hsp70 and, somewhat, of hsp90, two major heat shock response proteins. On the other hand, the
expression of hsc70, hsp20.4 and hsp20.1 was discontinuous in the latter part of diapause, or was
strongly controlled... by the diapause program and refractory to heat stress, as was the case for mtn
and fer, genes encoding two metal storage proteins crucial for metal ion homeostasis. This is the first
time that the effects of high winter temperatures have been assessed on cold-hardy diapausing larvae
and pupae of this important corn pest.
Кључне речи:
diapause / Ostrinia nubilalis / warm acclimation / heat stress / gene expression / heat shock proteins (HSPs) / antioxidative defense system (ADS)Извор:
International Journal of Molecular Sciences, 2024, 25, 3100-Издавач:
- Basel : MDPI
Финансирање / пројекти:
- info:eu-repo/grantAgreement/MESTD/inst- 451-03-66/2024-03/200125/RS// (RS-MESTD-inst- 451-03-66-2024-03-200125)
- nfo:eu-repo/grantAgreement/MESTD/inst- 451-03-65/2024-03/200125/RS//
- info:eu-repo/grantAgreement/MESTD/inst-451-03-66/2024-03/200358// (200358-MESTD-inst-451-03-66-2024-03)
Институција/група
MRIZPTY - JOUR AU - Uzelac, Iva AU - Avramov, Miloš AU - Knežić, Teodora AU - Tatić, Vanja AU - Gošić-Dondo, Snežana AU - Popović, Željko D. PY - 2024 UR - http://rik.mrizp.rs/handle/123456789/1403 AB - During diapause, a state of temporarily arrested development, insects require low winter temperatures to suppress their metabolism, conserve energy stores and acquire cold hardiness. A warmer winter could, thus, reduce diapause incidence and duration in many species, prematurely deplete their energy reserves and compromise post-diapause fitness. In this study, we investigated the combined effects of thermal stress and the diapause program on the expression of selected genes involved in antioxidant defense and heat shock response in the European corn borer Ostrinia nubilalis. By using qRT-PCR, it has been shown that response to chronic heat stress is characterized by raised mRNA levels of grx and trx, two important genes of the antioxidant defense system, as well as of hsp70 and, somewhat, of hsp90, two major heat shock response proteins. On the other hand, the expression of hsc70, hsp20.4 and hsp20.1 was discontinuous in the latter part of diapause, or was strongly controlled by the diapause program and refractory to heat stress, as was the case for mtn and fer, genes encoding two metal storage proteins crucial for metal ion homeostasis. This is the first time that the effects of high winter temperatures have been assessed on cold-hardy diapausing larvae and pupae of this important corn pest. PB - Basel : MDPI T2 - International Journal of Molecular Sciences T1 - Prolonged heat stress during winter diapause alters the expression of stress-response genes in Ostrinia nubilalis (Hbn.) VL - 25 SP - 3100 DO - 10.3390/ijms25063100 ER -
@article{ author = "Uzelac, Iva and Avramov, Miloš and Knežić, Teodora and Tatić, Vanja and Gošić-Dondo, Snežana and Popović, Željko D.", year = "2024", abstract = "During diapause, a state of temporarily arrested development, insects require low winter temperatures to suppress their metabolism, conserve energy stores and acquire cold hardiness. A warmer winter could, thus, reduce diapause incidence and duration in many species, prematurely deplete their energy reserves and compromise post-diapause fitness. In this study, we investigated the combined effects of thermal stress and the diapause program on the expression of selected genes involved in antioxidant defense and heat shock response in the European corn borer Ostrinia nubilalis. By using qRT-PCR, it has been shown that response to chronic heat stress is characterized by raised mRNA levels of grx and trx, two important genes of the antioxidant defense system, as well as of hsp70 and, somewhat, of hsp90, two major heat shock response proteins. On the other hand, the expression of hsc70, hsp20.4 and hsp20.1 was discontinuous in the latter part of diapause, or was strongly controlled by the diapause program and refractory to heat stress, as was the case for mtn and fer, genes encoding two metal storage proteins crucial for metal ion homeostasis. This is the first time that the effects of high winter temperatures have been assessed on cold-hardy diapausing larvae and pupae of this important corn pest.", publisher = "Basel : MDPI", journal = "International Journal of Molecular Sciences", title = "Prolonged heat stress during winter diapause alters the expression of stress-response genes in Ostrinia nubilalis (Hbn.)", volume = "25", pages = "3100", doi = "10.3390/ijms25063100" }
Uzelac, I., Avramov, M., Knežić, T., Tatić, V., Gošić-Dondo, S.,& Popović, Ž. D.. (2024). Prolonged heat stress during winter diapause alters the expression of stress-response genes in Ostrinia nubilalis (Hbn.). in International Journal of Molecular Sciences Basel : MDPI., 25, 3100. https://doi.org/10.3390/ijms25063100
Uzelac I, Avramov M, Knežić T, Tatić V, Gošić-Dondo S, Popović ŽD. Prolonged heat stress during winter diapause alters the expression of stress-response genes in Ostrinia nubilalis (Hbn.). in International Journal of Molecular Sciences. 2024;25:3100. doi:10.3390/ijms25063100 .
Uzelac, Iva, Avramov, Miloš, Knežić, Teodora, Tatić, Vanja, Gošić-Dondo, Snežana, Popović, Željko D., "Prolonged heat stress during winter diapause alters the expression of stress-response genes in Ostrinia nubilalis (Hbn.)" in International Journal of Molecular Sciences, 25 (2024):3100, https://doi.org/10.3390/ijms25063100 . .