MARKER-ASSISTED GА1-S INCORPORATION INTO WHITE MAIZE HYBRID´S PARENTAL LINES
UGRADNJA GА1-S GENA U RODITELJSKE LINIJE HIBRIDA KUKURUZA BELOG ZRNA POMOĆU MOLEKULARNIH MARKERA
Аутори
Kostadinović, MarijaRistić, Danijela
Božinović, Sofija
Djordjevic Melnik, Olivera
Ignjatović-Micić, Dragana
Vančetović, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Maize Research Institute Zemun Polje has a marker assisted backcross breeding (MABB) program aimed to incorporate the strong allele of incompatibility gene Gа1 into parental components of the hybrids with specific traits (white kernel). The main objective of this research was marker assisted development of the white maize hybrid´s parental lines with the complete cross-incompatibility to foreign pollens. Ga1-S-tightly-linked molecular markers successfully identified heterozygous plants with the percentage of approximately 50% after all three backcrossing. Regarding the background selection, 69% of progenies had recurrent parent’s genome (RPG) above the theoretical value for BC3 generation. Also, a few individuals even had 99%, the value theoretically achieved in BC6 generation, showing that MABB made a genetic gain in RPG recovery. Foreground selection in this generation aimed to identify homozygous dominant individuals. Out of 264 BC3F2 plants, 70 (26.5%) were dominant homozygotes, wh...ich is in accordance with the expected Mendelian ratio. Progenies of the BC3F2 homozygous dominant plants were planted alternatively with yellow-kernel maize of the same maturity to check cross-incompatibility. Unfortunately, none of the dominant progenies had a 100% white kernel, most likely due to the presence of modifier genes that increase/decrease the effectiveness of pollen exclusion or that, alternatively, modifiers were lost during the backcrossing. It could be expected that successfully employed functional markers (developed from the sequence polymorphisms present within functional gene(s) associated with phenotypic trait variations) would outbalance the noted impediments and enhance MABB efficiency to transfer the desired gene(s) controlling simple or complex trait(s) into cultivated varieties.
U Institutu za kukuruz “Zemun Polje” postoji program selekcije pomoću molekularnih markera (MAS) čiji je cilj ugradnja gena za inkompatibilnost Gа1-S u linije kukuruza koje su roditeljske komponente hibrida belog zrna. Glavni cilj ovog rada je bio stvaranje linija kukuruza belog
zrna sa potpunom unakrsnom inkompatibilnošću sa stranim polenom pomoću molekularnih
makera. Molekularnim markerima koji su blisko vezani za Ga1-S gen uspešno su identifikovane
heterozigotne biljke (50%) nakon sva tri povratna ukrštanja. Rezultati utvrđivanja procenta genoma rekurentnog roditelja (RPG) u BC3 generaciji pokazali su vrednost iznad očekivane kod 69%
potomstva, dok je kod određenog broja RPG iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Od 264 BC3
F2 pojedinačnih biljaka, 70 (26,5%) je bilo dominantno
homozigotno, što je u skladu sa očekivanim, prema pravilima Mendelovog nasleđivanja. Potomstva BC3
F2 dominantno homozigotnih biljaka posejana su sa kukuruzom... žutog zrna iste grupe
zrenja radi provere unakrsne inkompatibilnosti. Nijedno od dobijenih potomstava nije imalo 100%
belo zrno, najverovatnije usled prisustva gena modifikatora koji menjaju efikasnost isključivanja
polena, ili je došlo do gubljenja ovih gena prilikom povratnog ukrštanja. Moglo bi se očekivati da
će uspešna primena funkcionalnih markera (stvorenih na osnovu polimorfizama sekvenci unutar
samog gena vezanog za fenotipsku osobinu) prevazići uočene prepreke i poboljšati efikasnost MAS
za prenos željenih gena koji kontrolišu prostu ili složenu osobinu u gajene kulture.
Кључне речи:
background selection / foreground selection / Gа1-S / maize / marker assisted backcross breeding / kukuruz / selekcija pomoću molekularnih markeraИзвор:
Selekcija i semenarstvo, 2023, 29, 2, 17-24Издавач:
- Društvo selekcionera i semenara Republike Srbije, Beograd
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200040 (Институт за кукуруз 'Земун поље', Београд-Земун) (RS-MESTD-inst-2020-200040)
Институција/група
MRIZPTY - JOUR AU - Kostadinović, Marija AU - Ristić, Danijela AU - Božinović, Sofija AU - Djordjevic Melnik, Olivera AU - Ignjatović-Micić, Dragana AU - Vančetović, Jelena PY - 2023 UR - http://rik.mrizp.rs/handle/123456789/1393 AB - Maize Research Institute Zemun Polje has a marker assisted backcross breeding (MABB) program aimed to incorporate the strong allele of incompatibility gene Gа1 into parental components of the hybrids with specific traits (white kernel). The main objective of this research was marker assisted development of the white maize hybrid´s parental lines with the complete cross-incompatibility to foreign pollens. Ga1-S-tightly-linked molecular markers successfully identified heterozygous plants with the percentage of approximately 50% after all three backcrossing. Regarding the background selection, 69% of progenies had recurrent parent’s genome (RPG) above the theoretical value for BC3 generation. Also, a few individuals even had 99%, the value theoretically achieved in BC6 generation, showing that MABB made a genetic gain in RPG recovery. Foreground selection in this generation aimed to identify homozygous dominant individuals. Out of 264 BC3F2 plants, 70 (26.5%) were dominant homozygotes, which is in accordance with the expected Mendelian ratio. Progenies of the BC3F2 homozygous dominant plants were planted alternatively with yellow-kernel maize of the same maturity to check cross-incompatibility. Unfortunately, none of the dominant progenies had a 100% white kernel, most likely due to the presence of modifier genes that increase/decrease the effectiveness of pollen exclusion or that, alternatively, modifiers were lost during the backcrossing. It could be expected that successfully employed functional markers (developed from the sequence polymorphisms present within functional gene(s) associated with phenotypic trait variations) would outbalance the noted impediments and enhance MABB efficiency to transfer the desired gene(s) controlling simple or complex trait(s) into cultivated varieties. AB - U Institutu za kukuruz “Zemun Polje” postoji program selekcije pomoću molekularnih markera (MAS) čiji je cilj ugradnja gena za inkompatibilnost Gа1-S u linije kukuruza koje su roditeljske komponente hibrida belog zrna. Glavni cilj ovog rada je bio stvaranje linija kukuruza belog zrna sa potpunom unakrsnom inkompatibilnošću sa stranim polenom pomoću molekularnih makera. Molekularnim markerima koji su blisko vezani za Ga1-S gen uspešno su identifikovane heterozigotne biljke (50%) nakon sva tri povratna ukrštanja. Rezultati utvrđivanja procenta genoma rekurentnog roditelja (RPG) u BC3 generaciji pokazali su vrednost iznad očekivane kod 69% potomstva, dok je kod određenog broja RPG iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Od 264 BC3 F2 pojedinačnih biljaka, 70 (26,5%) je bilo dominantno homozigotno, što je u skladu sa očekivanim, prema pravilima Mendelovog nasleđivanja. Potomstva BC3 F2 dominantno homozigotnih biljaka posejana su sa kukuruzom žutog zrna iste grupe zrenja radi provere unakrsne inkompatibilnosti. Nijedno od dobijenih potomstava nije imalo 100% belo zrno, najverovatnije usled prisustva gena modifikatora koji menjaju efikasnost isključivanja polena, ili je došlo do gubljenja ovih gena prilikom povratnog ukrštanja. Moglo bi se očekivati da će uspešna primena funkcionalnih markera (stvorenih na osnovu polimorfizama sekvenci unutar samog gena vezanog za fenotipsku osobinu) prevazići uočene prepreke i poboljšati efikasnost MAS za prenos željenih gena koji kontrolišu prostu ili složenu osobinu u gajene kulture. PB - Društvo selekcionera i semenara Republike Srbije, Beograd T2 - Selekcija i semenarstvo T1 - MARKER-ASSISTED GА1-S INCORPORATION INTO WHITE MAIZE HYBRID´S PARENTAL LINES T1 - UGRADNJA GА1-S GENA U RODITELJSKE LINIJE HIBRIDA KUKURUZA BELOG ZRNA POMOĆU MOLEKULARNIH MARKERA VL - 29 IS - 2 SP - 17 EP - 24 DO - 10.5937/SelSem2302017K ER -
@article{ author = "Kostadinović, Marija and Ristić, Danijela and Božinović, Sofija and Djordjevic Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena", year = "2023", abstract = "Maize Research Institute Zemun Polje has a marker assisted backcross breeding (MABB) program aimed to incorporate the strong allele of incompatibility gene Gа1 into parental components of the hybrids with specific traits (white kernel). The main objective of this research was marker assisted development of the white maize hybrid´s parental lines with the complete cross-incompatibility to foreign pollens. Ga1-S-tightly-linked molecular markers successfully identified heterozygous plants with the percentage of approximately 50% after all three backcrossing. Regarding the background selection, 69% of progenies had recurrent parent’s genome (RPG) above the theoretical value for BC3 generation. Also, a few individuals even had 99%, the value theoretically achieved in BC6 generation, showing that MABB made a genetic gain in RPG recovery. Foreground selection in this generation aimed to identify homozygous dominant individuals. Out of 264 BC3F2 plants, 70 (26.5%) were dominant homozygotes, which is in accordance with the expected Mendelian ratio. Progenies of the BC3F2 homozygous dominant plants were planted alternatively with yellow-kernel maize of the same maturity to check cross-incompatibility. Unfortunately, none of the dominant progenies had a 100% white kernel, most likely due to the presence of modifier genes that increase/decrease the effectiveness of pollen exclusion or that, alternatively, modifiers were lost during the backcrossing. It could be expected that successfully employed functional markers (developed from the sequence polymorphisms present within functional gene(s) associated with phenotypic trait variations) would outbalance the noted impediments and enhance MABB efficiency to transfer the desired gene(s) controlling simple or complex trait(s) into cultivated varieties., U Institutu za kukuruz “Zemun Polje” postoji program selekcije pomoću molekularnih markera (MAS) čiji je cilj ugradnja gena za inkompatibilnost Gа1-S u linije kukuruza koje su roditeljske komponente hibrida belog zrna. Glavni cilj ovog rada je bio stvaranje linija kukuruza belog zrna sa potpunom unakrsnom inkompatibilnošću sa stranim polenom pomoću molekularnih makera. Molekularnim markerima koji su blisko vezani za Ga1-S gen uspešno su identifikovane heterozigotne biljke (50%) nakon sva tri povratna ukrštanja. Rezultati utvrđivanja procenta genoma rekurentnog roditelja (RPG) u BC3 generaciji pokazali su vrednost iznad očekivane kod 69% potomstva, dok je kod određenog broja RPG iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Od 264 BC3 F2 pojedinačnih biljaka, 70 (26,5%) je bilo dominantno homozigotno, što je u skladu sa očekivanim, prema pravilima Mendelovog nasleđivanja. Potomstva BC3 F2 dominantno homozigotnih biljaka posejana su sa kukuruzom žutog zrna iste grupe zrenja radi provere unakrsne inkompatibilnosti. Nijedno od dobijenih potomstava nije imalo 100% belo zrno, najverovatnije usled prisustva gena modifikatora koji menjaju efikasnost isključivanja polena, ili je došlo do gubljenja ovih gena prilikom povratnog ukrštanja. Moglo bi se očekivati da će uspešna primena funkcionalnih markera (stvorenih na osnovu polimorfizama sekvenci unutar samog gena vezanog za fenotipsku osobinu) prevazići uočene prepreke i poboljšati efikasnost MAS za prenos željenih gena koji kontrolišu prostu ili složenu osobinu u gajene kulture.", publisher = "Društvo selekcionera i semenara Republike Srbije, Beograd", journal = "Selekcija i semenarstvo", title = "MARKER-ASSISTED GА1-S INCORPORATION INTO WHITE MAIZE HYBRID´S PARENTAL LINES, UGRADNJA GА1-S GENA U RODITELJSKE LINIJE HIBRIDA KUKURUZA BELOG ZRNA POMOĆU MOLEKULARNIH MARKERA", volume = "29", number = "2", pages = "17-24", doi = "10.5937/SelSem2302017K" }
Kostadinović, M., Ristić, D., Božinović, S., Djordjevic Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2023). MARKER-ASSISTED GА1-S INCORPORATION INTO WHITE MAIZE HYBRID´S PARENTAL LINES. in Selekcija i semenarstvo Društvo selekcionera i semenara Republike Srbije, Beograd., 29(2), 17-24. https://doi.org/10.5937/SelSem2302017K
Kostadinović M, Ristić D, Božinović S, Djordjevic Melnik O, Ignjatović-Micić D, Vančetović J. MARKER-ASSISTED GА1-S INCORPORATION INTO WHITE MAIZE HYBRID´S PARENTAL LINES. in Selekcija i semenarstvo. 2023;29(2):17-24. doi:10.5937/SelSem2302017K .
Kostadinović, Marija, Ristić, Danijela, Božinović, Sofija, Djordjevic Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "MARKER-ASSISTED GА1-S INCORPORATION INTO WHITE MAIZE HYBRID´S PARENTAL LINES" in Selekcija i semenarstvo, 29, no. 2 (2023):17-24, https://doi.org/10.5937/SelSem2302017K . .