Effects of climate change on mycopopulations on cereal grain in Serbia
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In recent years, global climate variability and changes have affected agro climatic conditions, which have contributed to the spread of new fungal
species in cereal grains in Serbia. These changes can affect the synthesis of
higher concentrations of mycotoxins in cereal grains during the growing
season and can cause economic losses in production, as well as increased
risk to human and animal health. The following new species of the genera
Fusarium and Aspergillus have been identified in cereal grains in Serbia:
Fusarium vorosii in wheat, Fusarium boothii in maize, Fusarium
verticillioides in triticale and wheat, as well as the species Aspergillus
parasiticus in maize, wheat and barley. These species have been identified
on the basis of morphological, pathogenic, toxicological and molecular
methods. DNA isolation was performed using DNeasy Plant Mini Kit-a.
Species F. vorosii and F. boothii were detected using three genomic regions
(TEF−1α, histone H3 and β−tubul...in) that were sequenced and obtained
sequences were deposited in NCBI. A. parasiticus was detected by the RFLP
method using a primer pair IGS-F/IGS-R specific for the IGS region of genes
aflJ and aflR involved in the aflatoxin biosynthesis. A. parasiticus CBS
100926 was used as a reference isolate. In order to prove the presence of the
species F. verticillioides in triticale, a pair of primers FV-F2/FV-R was used.
This pair of primers amplifies the sequence of the gaoB gene, and proved to
be specific for the stated species. Moreover, for the same purpose, a pair of
primers VER1-VER2 designed based on the calmodulin partial gene was
used. The stated reasons, as well as the fact that new species of the Fg
complex had been identified in the surrounding of Serbia, have indicated the
need for continuous monitoring of these toxigenic species in the production
of cereals.
Кључне речи:
F. vorosii / F. boothii / F. verticillioides / A. parasiticus / climate changeИзвор:
The Frontiers of Science and Technology in Crop Breeding and Production Conference, 2021, 51-Издавач:
- Maize Research Institute, Zemun Polje