A high level of genetic purity in crop varieties must be achieved and maintained for
agronomic performance, encouraging investment and innovation in plant breeding and ensuring that
the improvements in productivity and quality imparted by breeders are delivered to the consumer.
Since the success of hybrid seed production is dependent upon the genetic purity of the parental lines,
in this study, the experimental F1exp maize hybrid and its parental inbreeds were used as a model
system to examine the discriminative power of morphological, biochemical and SSR markers for
seed purity assay. The highest number of off-type plants was estimated by morphological markers.
According to the comparison of prolamins and albumins banding patterns of parental and derived
F1exp seeds, genetic impurities could not be detected. Molecular analysis detected two types of
genetic profile irregularity. Beside its use for verifying varieties of maize, report on umc1545 primer
pair ability to detect n...on-specific bands (i.e., off-types), in both the maternal component and F1exp,
which is the first report on this issue yet, strongly supports the recommendation of this SSR marker
use for more accurate and time-efficient maize hybrids and parental lines genetic pyrity testing
Keywords:
phenotypic uniformity / seed storage proteins / SSRs / ultrathin-layer isoelectric focusing / Zea mays L.
TY - JOUR
AU - Kovinčić, Anika
AU - Marković, Ksenija
AU - Ristić, Danijela
AU - Babić, Vojka
AU - Petrović, Tanja
AU - Živanović, Tomislav
AU - Kravić, Natalija
PY - 2023
UR - http://rik.mrizp.rs/handle/123456789/1172
AB - A high level of genetic purity in crop varieties must be achieved and maintained for
agronomic performance, encouraging investment and innovation in plant breeding and ensuring that
the improvements in productivity and quality imparted by breeders are delivered to the consumer.
Since the success of hybrid seed production is dependent upon the genetic purity of the parental lines,
in this study, the experimental F1exp maize hybrid and its parental inbreeds were used as a model
system to examine the discriminative power of morphological, biochemical and SSR markers for
seed purity assay. The highest number of off-type plants was estimated by morphological markers.
According to the comparison of prolamins and albumins banding patterns of parental and derived
F1exp seeds, genetic impurities could not be detected. Molecular analysis detected two types of
genetic profile irregularity. Beside its use for verifying varieties of maize, report on umc1545 primer
pair ability to detect non-specific bands (i.e., off-types), in both the maternal component and F1exp,
which is the first report on this issue yet, strongly supports the recommendation of this SSR marker
use for more accurate and time-efficient maize hybrids and parental lines genetic pyrity testing
PB - Basel : MDPI
T2 - Genes
T1 - Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation
VL - 14
IS - 6
EP - 1195
DO - 10.3390/genes14061195
ER -
@article{
author = "Kovinčić, Anika and Marković, Ksenija and Ristić, Danijela and Babić, Vojka and Petrović, Tanja and Živanović, Tomislav and Kravić, Natalija",
year = "2023",
abstract = "A high level of genetic purity in crop varieties must be achieved and maintained for
agronomic performance, encouraging investment and innovation in plant breeding and ensuring that
the improvements in productivity and quality imparted by breeders are delivered to the consumer.
Since the success of hybrid seed production is dependent upon the genetic purity of the parental lines,
in this study, the experimental F1exp maize hybrid and its parental inbreeds were used as a model
system to examine the discriminative power of morphological, biochemical and SSR markers for
seed purity assay. The highest number of off-type plants was estimated by morphological markers.
According to the comparison of prolamins and albumins banding patterns of parental and derived
F1exp seeds, genetic impurities could not be detected. Molecular analysis detected two types of
genetic profile irregularity. Beside its use for verifying varieties of maize, report on umc1545 primer
pair ability to detect non-specific bands (i.e., off-types), in both the maternal component and F1exp,
which is the first report on this issue yet, strongly supports the recommendation of this SSR marker
use for more accurate and time-efficient maize hybrids and parental lines genetic pyrity testing",
publisher = "Basel : MDPI",
journal = "Genes",
title = "Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation",
volume = "14",
number = "6",
pages = "1195",
doi = "10.3390/genes14061195"
}
Kovinčić, A., Marković, K., Ristić, D., Babić, V., Petrović, T., Živanović, T.,& Kravić, N.. (2023). Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation. in Genes
Basel : MDPI., 14(6).
https://doi.org/10.3390/genes14061195
Kovinčić A, Marković K, Ristić D, Babić V, Petrović T, Živanović T, Kravić N. Efficiency of Biological Typing Methods in Maize Hybrid Genetic Purity Estimation. in Genes. 2023;14(6):null-1195.
doi:10.3390/genes14061195 .
