Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages
Аутори
Božić, ManjaBanović Đeri, Bojana
Dudić, Dragana
Ignjatović-Micić, Dragana
Vančetović, Jelena
Delić, Nenad
Nikolić, Ana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Earlier sowing is one of the most important strategies of ensuring good yield potential and crop quality under poor environmental conditions arising as consequences of climate changes. Sowing maize in early spring, when the temperatures are lower, enables avoidance of drought and high summer temperatures during the flowering and grain filling stages, but it also means that maize plants will be exposed to suboptimal temperatures during earlier developmental stages. Consequently, development of maize lines tolerant to low temperatures during those stages becomes precedence.
An initial study encompassed whole transcriptome sequencing of 46 maize inbred lines at V4 stage grown under optimal temperature conditions. Gene expression analysis of maize genotypes grouped as Lancaster and non-Lancaster (BSSS, Iowa dent, etc.) revealed a set of 77 differentially expressed genes (DEGs). Seven of these genes, related to abiotic stress response, were further characterized under low temperature cond...itions in eight inbred lines at V4 stage. Their expression showed specific profiles depending on the duration of low temperature exposure and genetic background. To test if these genes follow the same expression patterns in earlier developmental stages, the experiment was performed with 5-day old maize seedlings of two inbred lines (tolerant and sensitive), under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. Five analyzed genes showed different expression regulation dependent on cold exposure duration. Two genes showed regulation dependent both on cold exposure duration and genetic background. Additionally, three of five DEGs showed different expression patterns at 5-day old seedling stage than at V4 stage. The results imply that processes underlying maize low temperature response are differentially regulated based on stress exposure duration, developmental stage and genetic background.
Кључне речи:
DEGs / maize / low temperature tolerance / 5-day old seedlingsИзвор:
International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts, 2021Издавач:
- Belgrade : Maize Research Institute "Zemun Polje"
Институција/група
MRIZPTY - CONF AU - Božić, Manja AU - Banović Đeri, Bojana AU - Dudić, Dragana AU - Ignjatović-Micić, Dragana AU - Vančetović, Jelena AU - Delić, Nenad AU - Nikolić, Ana PY - 2021 UR - http://rik.mrizp.rs/handle/123456789/1146 AB - Earlier sowing is one of the most important strategies of ensuring good yield potential and crop quality under poor environmental conditions arising as consequences of climate changes. Sowing maize in early spring, when the temperatures are lower, enables avoidance of drought and high summer temperatures during the flowering and grain filling stages, but it also means that maize plants will be exposed to suboptimal temperatures during earlier developmental stages. Consequently, development of maize lines tolerant to low temperatures during those stages becomes precedence. An initial study encompassed whole transcriptome sequencing of 46 maize inbred lines at V4 stage grown under optimal temperature conditions. Gene expression analysis of maize genotypes grouped as Lancaster and non-Lancaster (BSSS, Iowa dent, etc.) revealed a set of 77 differentially expressed genes (DEGs). Seven of these genes, related to abiotic stress response, were further characterized under low temperature conditions in eight inbred lines at V4 stage. Their expression showed specific profiles depending on the duration of low temperature exposure and genetic background. To test if these genes follow the same expression patterns in earlier developmental stages, the experiment was performed with 5-day old maize seedlings of two inbred lines (tolerant and sensitive), under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. Five analyzed genes showed different expression regulation dependent on cold exposure duration. Two genes showed regulation dependent both on cold exposure duration and genetic background. Additionally, three of five DEGs showed different expression patterns at 5-day old seedling stage than at V4 stage. The results imply that processes underlying maize low temperature response are differentially regulated based on stress exposure duration, developmental stage and genetic background. PB - Belgrade : Maize Research Institute "Zemun Polje" C3 - International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts T1 - Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages UR - https://hdl.handle.net/21.15107/rcub_rik_1146 ER -
@conference{ author = "Božić, Manja and Banović Đeri, Bojana and Dudić, Dragana and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana", year = "2021", abstract = "Earlier sowing is one of the most important strategies of ensuring good yield potential and crop quality under poor environmental conditions arising as consequences of climate changes. Sowing maize in early spring, when the temperatures are lower, enables avoidance of drought and high summer temperatures during the flowering and grain filling stages, but it also means that maize plants will be exposed to suboptimal temperatures during earlier developmental stages. Consequently, development of maize lines tolerant to low temperatures during those stages becomes precedence. An initial study encompassed whole transcriptome sequencing of 46 maize inbred lines at V4 stage grown under optimal temperature conditions. Gene expression analysis of maize genotypes grouped as Lancaster and non-Lancaster (BSSS, Iowa dent, etc.) revealed a set of 77 differentially expressed genes (DEGs). Seven of these genes, related to abiotic stress response, were further characterized under low temperature conditions in eight inbred lines at V4 stage. Their expression showed specific profiles depending on the duration of low temperature exposure and genetic background. To test if these genes follow the same expression patterns in earlier developmental stages, the experiment was performed with 5-day old maize seedlings of two inbred lines (tolerant and sensitive), under optimal (25°/20°C) and low (8°/10°C) temperature conditions, with a 12h photoperiod. Samples for RNA extraction, cDNA synthesis and qPCR expression analysis were taken after 6h and 24h exposure to experimental temperatures. Five analyzed genes showed different expression regulation dependent on cold exposure duration. Two genes showed regulation dependent both on cold exposure duration and genetic background. Additionally, three of five DEGs showed different expression patterns at 5-day old seedling stage than at V4 stage. The results imply that processes underlying maize low temperature response are differentially regulated based on stress exposure duration, developmental stage and genetic background.", publisher = "Belgrade : Maize Research Institute "Zemun Polje"", journal = "International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts", title = "Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages", url = "https://hdl.handle.net/21.15107/rcub_rik_1146" }
Božić, M., Banović Đeri, B., Dudić, D., Ignjatović-Micić, D., Vančetović, J., Delić, N.,& Nikolić, A.. (2021). Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages. in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts Belgrade : Maize Research Institute "Zemun Polje".. https://hdl.handle.net/21.15107/rcub_rik_1146
Božić M, Banović Đeri B, Dudić D, Ignjatović-Micić D, Vančetović J, Delić N, Nikolić A. Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages. in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts. 2021;. https://hdl.handle.net/21.15107/rcub_rik_1146 .
Božić, Manja, Banović Đeri, Bojana, Dudić, Dragana, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Several genes involved in low temperature response in maize follow different expression patterns at different developmental stages" in International Conference The Frontiers of Science and Technology in Crop Breeding and Production Conference, Belgrade, Serbia, 8 – 9 June, 2021. Book of Abstracts (2021), https://hdl.handle.net/21.15107/rcub_rik_1146 .