Expression analysis of cold induced genes in early developmental stages of maize
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A large number of strategies in crop production today is focussed on ensuring a decrease in yield loss occurring as a result of the increasing temperatures and drought arising during late spring and summer, which are only some of the consequences of climate change. One of those strategies is earlier sowing, during the colder months in early spring, which means that finding methods that quickly and accurately estimate low temperature tolerance of different crops, including maize, is becoming increasingly important. Our idea was to use the expression of Zea mays sp. cold induced genes (ZmCOI) for this purpose, and to see if there is a significant correlation between the quantities of certain ZmCOI mRNA and low temperature tolerance. This was done through an experiment that included growing several maize lines known from breeders experience to be sensitive or tolerant to cold, under optimal and low temperatures, 22° C and 6° C, respectively. Samples (plant leaves) were taken during the V4... growth stage, at different time points during the treatment (6h and 24h) and used for RNA extraction, cDNA synthesis and qPCR expression analysis for the selected genes (ZmDREB2A, ZmERF3, ZmACA1). Preliminary results showed changes in the expression of these genes between chosen sensitive and tolerant inbred lines. These results provide a good basis for further studies with more cold induced genes and inbred lines that will hopefully confirm their effectiveness as a tool for determination of tolerance to low temperatures.
Кључне речи:
maize / cold stress / relative gene expression / cold indused genesИзвор:
12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts, 2021Издавач:
- East Sarajevo : Faculty of agriculture
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Генетички ресурси кукуруза као извор побољшаног квалитета зрна и толерантности према суши (RS-MESTD-Technological Development (TD or TR)-31028)
Институција/група
MRIZPTY - CONF AU - Božić, Manja AU - Ignjatović-Micić, Dragana AU - Vancetovic, Jelena AU - Markovic, Ksenija AU - Nikolic, Ana PY - 2021 UR - http://rik.mrizp.rs/handle/123456789/1143 AB - A large number of strategies in crop production today is focussed on ensuring a decrease in yield loss occurring as a result of the increasing temperatures and drought arising during late spring and summer, which are only some of the consequences of climate change. One of those strategies is earlier sowing, during the colder months in early spring, which means that finding methods that quickly and accurately estimate low temperature tolerance of different crops, including maize, is becoming increasingly important. Our idea was to use the expression of Zea mays sp. cold induced genes (ZmCOI) for this purpose, and to see if there is a significant correlation between the quantities of certain ZmCOI mRNA and low temperature tolerance. This was done through an experiment that included growing several maize lines known from breeders experience to be sensitive or tolerant to cold, under optimal and low temperatures, 22° C and 6° C, respectively. Samples (plant leaves) were taken during the V4 growth stage, at different time points during the treatment (6h and 24h) and used for RNA extraction, cDNA synthesis and qPCR expression analysis for the selected genes (ZmDREB2A, ZmERF3, ZmACA1). Preliminary results showed changes in the expression of these genes between chosen sensitive and tolerant inbred lines. These results provide a good basis for further studies with more cold induced genes and inbred lines that will hopefully confirm their effectiveness as a tool for determination of tolerance to low temperatures. PB - East Sarajevo : Faculty of agriculture C3 - 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts T1 - Expression analysis of cold induced genes in early developmental stages of maize UR - https://hdl.handle.net/21.15107/rcub_rik_1143 ER -
@conference{ author = "Božić, Manja and Ignjatović-Micić, Dragana and Vancetovic, Jelena and Markovic, Ksenija and Nikolic, Ana", year = "2021", abstract = "A large number of strategies in crop production today is focussed on ensuring a decrease in yield loss occurring as a result of the increasing temperatures and drought arising during late spring and summer, which are only some of the consequences of climate change. One of those strategies is earlier sowing, during the colder months in early spring, which means that finding methods that quickly and accurately estimate low temperature tolerance of different crops, including maize, is becoming increasingly important. Our idea was to use the expression of Zea mays sp. cold induced genes (ZmCOI) for this purpose, and to see if there is a significant correlation between the quantities of certain ZmCOI mRNA and low temperature tolerance. This was done through an experiment that included growing several maize lines known from breeders experience to be sensitive or tolerant to cold, under optimal and low temperatures, 22° C and 6° C, respectively. Samples (plant leaves) were taken during the V4 growth stage, at different time points during the treatment (6h and 24h) and used for RNA extraction, cDNA synthesis and qPCR expression analysis for the selected genes (ZmDREB2A, ZmERF3, ZmACA1). Preliminary results showed changes in the expression of these genes between chosen sensitive and tolerant inbred lines. These results provide a good basis for further studies with more cold induced genes and inbred lines that will hopefully confirm their effectiveness as a tool for determination of tolerance to low temperatures.", publisher = "East Sarajevo : Faculty of agriculture", journal = "12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts", title = "Expression analysis of cold induced genes in early developmental stages of maize", url = "https://hdl.handle.net/21.15107/rcub_rik_1143" }
Božić, M., Ignjatović-Micić, D., Vancetovic, J., Markovic, K.,& Nikolic, A.. (2021). Expression analysis of cold induced genes in early developmental stages of maize. in 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts East Sarajevo : Faculty of agriculture.. https://hdl.handle.net/21.15107/rcub_rik_1143
Božić M, Ignjatović-Micić D, Vancetovic J, Markovic K, Nikolic A. Expression analysis of cold induced genes in early developmental stages of maize. in 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts. 2021;. https://hdl.handle.net/21.15107/rcub_rik_1143 .
Božić, Manja, Ignjatović-Micić, Dragana, Vancetovic, Jelena, Markovic, Ksenija, Nikolic, Ana, "Expression analysis of cold induced genes in early developmental stages of maize" in 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts (2021), https://hdl.handle.net/21.15107/rcub_rik_1143 .