Bioinformatics prediction of mitochondrial and chloroplast transcripts possibly involved in maize abiotic stress signaling
Authors
Banović Đeri, BojanaDžodanović, Lana
Janić, Pavle
Vančetović, Jelena

Ignjatović-Micić, Dragana

Samardžić, Jelena
Nikolić, Ana

Conference object (Published version)
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Show full item recordAbstract
In plants, the expression of mitochondrial and chloroplast genomes is continuously changing
to enable adequate functional adjustments related to overall metabolic changes needed for
plant adaptation to fluctuating environmental conditions. In order to identify mitochondrial
and chloroplast transcripts expressed prior to stresses, we have analyzed mitochondrial and
chloroplast transcriptomes of 46 maize inbred lines chosen from three main heterotic groups
(BSSS, Lancaster, Iowa dent) regularly used in elite maize breeding programs. Organelles’
data were extracted from transcriptome data obtained by next generation sequencing of
total leaf transcriptomes of 46 maize inbred lines grown under the optimal conditions in the
greenhouse. All data manipulation and differential gene expression analysis were performed
by using Galaxy software (https://usegalaxy.eu/). Detected organellar complex transcripts
milieus were expected to contain candidates that modulate responses of their geno...mes
by activating certain signaling pathways upon exposure to abiotic stresses. With the aim
to identify such candidates we focused on selecting transcripts that were differentially
expressed between 46 maize inbred lines grown under the optimal breeding conditions and
also annotated as important for abiotic stresses response in different plant species. Selected
transcripts/genes were then subjected to freely available tools for gene networking analysis
with the aim to depict possible anterograde and retrograde signaling between nucleus and
mitochondria/chloroplasts under the abiotic stresses. Obtained results are promising in terms
of planning biological experiments for testing the roles of genes that were bioinformatically
predicted as novel members of abiotic stress signaling network between nucleus and
organelles in maize.
Keywords:
organelle / transcriptome / abiotic stress / maizeSource:
Book of Abstracts, 6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia, 2019, 264-264Publisher:
- Belgrade : Serbian Genetics Society