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Genotypic variability of root and shoot traits of bread wheat (Triticum aestivum L.) at seedling stage

Blažić, Milica; Dodig, Dejan; Kandić, Vesna; Đokić, Dragoslav; Živanović, Tomislav

(Beograd : Društvo genetičara Srbija, 2021)

TY  - JOUR
AU  - Blažić, Milica
AU  - Dodig, Dejan
AU  - Kandić, Vesna
AU  - Đokić, Dragoslav
AU  - Živanović, Tomislav
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/831
AB  - The evaluation of the embryonic root and stem of bread wheat (Triticum aestivum L.) in the early stage of development (seedling stage) can be a powerful tool in wheat breeding aimed at obtaining progenies with a greater early vigour. It is revealed that genotypes with faster early vigour have produced higher biomass and grain yield. In this study, the evaluation of traits of the embryonic root and the embryonic stem of 101 bread wheat genotypes was preformed at the 10-day old seedlings. The following eight morphological traits of roots and stems were analysed: primary root length, branching interval, the number of roots, total length of lateral roots, angle of seminal roots, stem length, root dry weight and the stem dry weight. Analysed lateral roots included seminal roots. The greatest, i.e. the smallest variability of observed traits was detected in the branching interval, i.e. the stem length, respectively. The highest positive correlation was determined between the primary root length and the total length of lateral roots. The cluster analysis, based on observed traits, shows that genotypes were clearly divided into two main clusters, A and B. The two clusters essentially differed from each other in the values of the following traits: primary root length, total length of lateral roots, root dry weight, stem dry weight and the stem length. Genotypes with shorter primary and lateral roots, lower root and stem dry weight and a shorter stem were grouped in the cluster B. On the other hand, the cluster A encompassed genotypes with values of these traits above or around the average. The values of the remaining analysed traits: the angle of seminal roots, the number of lateral roots and the branching interval varied greatly between obtained clusters. The cluster analysis showed the homogeneity of genotypes originating from Serbia and the region; their values of the root and stem length and weight were mostly around and below the average. However, the values of the angle of seminal roots, number of lateral roots and the branching interval were above average.
AB  - Ocena svojstava korena i stabla hlebne pšenice (Triticum aestivum L.) u ranoj fazi razvića, na stupnju klijanaca, može biti moćan alat u oplemenjivanju pšenice sa ciljem dobijanja potomstva sa bržim ranim porastom. Pokazano je da su genotipovi sa bržim ranim porastom proizveli veću biomasu i prinos zrna. U ovoj studiji izvršena je ocena svojstava korena i stabla 101 genotipa hlebne pšenice u fazi klijanaca starosti 10 dana. Analizirano je 8 morfoloških svojstava korena i stabla: dužina primarnog korena, distanca do prve grane na primarnom korenu, broj bočnih korenova, ukupna dužina bočnih korenova, ugao seminalnih korenova, dužina stabla, suva masa korena i suva masa stabla. Analizirani bočni korenovi podrazumevali su seminalne korenove. Najveću varijabilnost od ispitivanih osobina imala je distanca do prve grane na primarnom korenu, a najmanju dužina stabla. Najveća pozitivna korelacija je utvrđena između dužine primarnog korena i ukupne dužine bočnih korenova. Klaster analiza pokazala je homogenost genotipova poreklom iz Srbije i regiona; njihove vrednosti dužine i težine korena i stabla bile su uglavnom oko i ispod proseka. Međutim, vrednosti za osobine: ugao seminalnih korenova, broj bočnih korenova i distanca do prve grane na primarnom korenu, bile su iznad proseka.
PB  - Beograd : Društvo genetičara Srbija
T2  - Genetika
T1  - Genotypic variability of root and shoot traits of bread wheat (Triticum aestivum L.) at seedling stage
VL  - 52
IS  - 2
SP  - 687
EP  - 702
DO  - 10.2298/GENSR2102687B
ER  - 
@article{
author = "Blažić, Milica and Dodig, Dejan and Kandić, Vesna and Đokić, Dragoslav and Živanović, Tomislav",
year = "2021",
abstract = "The evaluation of the embryonic root and stem of bread wheat (Triticum aestivum L.) in the early stage of development (seedling stage) can be a powerful tool in wheat breeding aimed at obtaining progenies with a greater early vigour. It is revealed that genotypes with faster early vigour have produced higher biomass and grain yield. In this study, the evaluation of traits of the embryonic root and the embryonic stem of 101 bread wheat genotypes was preformed at the 10-day old seedlings. The following eight morphological traits of roots and stems were analysed: primary root length, branching interval, the number of roots, total length of lateral roots, angle of seminal roots, stem length, root dry weight and the stem dry weight. Analysed lateral roots included seminal roots. The greatest, i.e. the smallest variability of observed traits was detected in the branching interval, i.e. the stem length, respectively. The highest positive correlation was determined between the primary root length and the total length of lateral roots. The cluster analysis, based on observed traits, shows that genotypes were clearly divided into two main clusters, A and B. The two clusters essentially differed from each other in the values of the following traits: primary root length, total length of lateral roots, root dry weight, stem dry weight and the stem length. Genotypes with shorter primary and lateral roots, lower root and stem dry weight and a shorter stem were grouped in the cluster B. On the other hand, the cluster A encompassed genotypes with values of these traits above or around the average. The values of the remaining analysed traits: the angle of seminal roots, the number of lateral roots and the branching interval varied greatly between obtained clusters. The cluster analysis showed the homogeneity of genotypes originating from Serbia and the region; their values of the root and stem length and weight were mostly around and below the average. However, the values of the angle of seminal roots, number of lateral roots and the branching interval were above average., Ocena svojstava korena i stabla hlebne pšenice (Triticum aestivum L.) u ranoj fazi razvića, na stupnju klijanaca, može biti moćan alat u oplemenjivanju pšenice sa ciljem dobijanja potomstva sa bržim ranim porastom. Pokazano je da su genotipovi sa bržim ranim porastom proizveli veću biomasu i prinos zrna. U ovoj studiji izvršena je ocena svojstava korena i stabla 101 genotipa hlebne pšenice u fazi klijanaca starosti 10 dana. Analizirano je 8 morfoloških svojstava korena i stabla: dužina primarnog korena, distanca do prve grane na primarnom korenu, broj bočnih korenova, ukupna dužina bočnih korenova, ugao seminalnih korenova, dužina stabla, suva masa korena i suva masa stabla. Analizirani bočni korenovi podrazumevali su seminalne korenove. Najveću varijabilnost od ispitivanih osobina imala je distanca do prve grane na primarnom korenu, a najmanju dužina stabla. Najveća pozitivna korelacija je utvrđena između dužine primarnog korena i ukupne dužine bočnih korenova. Klaster analiza pokazala je homogenost genotipova poreklom iz Srbije i regiona; njihove vrednosti dužine i težine korena i stabla bile su uglavnom oko i ispod proseka. Međutim, vrednosti za osobine: ugao seminalnih korenova, broj bočnih korenova i distanca do prve grane na primarnom korenu, bile su iznad proseka.",
publisher = "Beograd : Društvo genetičara Srbija",
journal = "Genetika",
title = "Genotypic variability of root and shoot traits of bread wheat (Triticum aestivum L.) at seedling stage",
volume = "52",
number = "2",
pages = "687-702",
doi = "10.2298/GENSR2102687B"
}
Blažić, M., Dodig, D., Kandić, V., Đokić, D.,& Živanović, T.. (2021). Genotypic variability of root and shoot traits of bread wheat (Triticum aestivum L.) at seedling stage. in Genetika
Beograd : Društvo genetičara Srbija., 52(2), 687-702.
https://doi.org/10.2298/GENSR2102687B
Blažić M, Dodig D, Kandić V, Đokić D, Živanović T. Genotypic variability of root and shoot traits of bread wheat (Triticum aestivum L.) at seedling stage. in Genetika. 2021;52(2):687-702.
doi:10.2298/GENSR2102687B .
Blažić, Milica, Dodig, Dejan, Kandić, Vesna, Đokić, Dragoslav, Živanović, Tomislav, "Genotypic variability of root and shoot traits of bread wheat (Triticum aestivum L.) at seedling stage" in Genetika, 52, no. 2 (2021):687-702,
https://doi.org/10.2298/GENSR2102687B . .

The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain

Savic, Iva; Nikolic, Milica; Vico, Ivana; Mladenović Drinić, Snežana; Duduk, Natasa; Stanković, Slavica

(Beograd : Društvo genetičara Srbija, 2021)

TY  - JOUR
AU  - Savic, Iva
AU  - Nikolic, Milica
AU  - Vico, Ivana
AU  - Mladenović Drinić, Snežana
AU  - Duduk, Natasa
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/830
AB  - During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.
AB  - Tokom perioda od 2014. do 2017. godine sakupljani su uzorci pšenice sa fuzarioznih klasova sa 20 lokacija u Srbiji. Nakon izolacije, gljive su gajene na KDA podlozi 7 dana u termostatu na temperaturi od 25oC. Na osnovu identifikacije in situ na KDA (krompir dekstrozni agar), 36 izolata sekcije Liseola odabrano je za dalje analize. Potvrđena je patogenost svih izolata na listovima pšenice. Virulentnost izolata utvrđena je merenjem dužina formirane pege na mestu inokulacije. Za dokazivanje prisustva vrste Fusarium verticillioides korišćen je par prajmera FV-F2/FV-R koji umnožava sekvence gaoB gena i koji se pokazao kao specifičan za navedenu vrstu. Takođe, sa istim ciljem korišćen je par prajmera VER1-VER2 dizajniran na osnovu dela genske sekvence za kalmodulin. Kao pozitivna kontrola korišćen je referentni izolat RBG 1603 Q27. Par prajmera VER1-VER2 obrazovao je traku očekivane veličine (578bp) kod 18 izolata, dok je pomoću para prajmera FV-F2/FV-R traka dužine 370bp potvrdila prisustvo F. verticillioides kod 16 izolata. Šesnaest od 18 izolata koji su identifikovani VER1-VER2 parom, takođe su identifikovani i FV-FS/FV-R prajmerima. U negativnoj kontroli nije bilo amplifikacije.
PB  - Beograd : Društvo genetičara Srbija
T2  - Genetika
T1  - The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain
VL  - 53
IS  - 2
SP  - 641
EP  - 649
DO  - 10.2298/GENSR2102641S
ER  - 
@article{
author = "Savic, Iva and Nikolic, Milica and Vico, Ivana and Mladenović Drinić, Snežana and Duduk, Natasa and Stanković, Slavica",
year = "2021",
abstract = "During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control., Tokom perioda od 2014. do 2017. godine sakupljani su uzorci pšenice sa fuzarioznih klasova sa 20 lokacija u Srbiji. Nakon izolacije, gljive su gajene na KDA podlozi 7 dana u termostatu na temperaturi od 25oC. Na osnovu identifikacije in situ na KDA (krompir dekstrozni agar), 36 izolata sekcije Liseola odabrano je za dalje analize. Potvrđena je patogenost svih izolata na listovima pšenice. Virulentnost izolata utvrđena je merenjem dužina formirane pege na mestu inokulacije. Za dokazivanje prisustva vrste Fusarium verticillioides korišćen je par prajmera FV-F2/FV-R koji umnožava sekvence gaoB gena i koji se pokazao kao specifičan za navedenu vrstu. Takođe, sa istim ciljem korišćen je par prajmera VER1-VER2 dizajniran na osnovu dela genske sekvence za kalmodulin. Kao pozitivna kontrola korišćen je referentni izolat RBG 1603 Q27. Par prajmera VER1-VER2 obrazovao je traku očekivane veličine (578bp) kod 18 izolata, dok je pomoću para prajmera FV-F2/FV-R traka dužine 370bp potvrdila prisustvo F. verticillioides kod 16 izolata. Šesnaest od 18 izolata koji su identifikovani VER1-VER2 parom, takođe su identifikovani i FV-FS/FV-R prajmerima. U negativnoj kontroli nije bilo amplifikacije.",
publisher = "Beograd : Društvo genetičara Srbija",
journal = "Genetika",
title = "The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain",
volume = "53",
number = "2",
pages = "641-649",
doi = "10.2298/GENSR2102641S"
}
Savic, I., Nikolic, M., Vico, I., Mladenović Drinić, S., Duduk, N.,& Stanković, S.. (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. in Genetika
Beograd : Društvo genetičara Srbija., 53(2), 641-649.
https://doi.org/10.2298/GENSR2102641S
Savic I, Nikolic M, Vico I, Mladenović Drinić S, Duduk N, Stanković S. The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. in Genetika. 2021;53(2):641-649.
doi:10.2298/GENSR2102641S .
Savic, Iva, Nikolic, Milica, Vico, Ivana, Mladenović Drinić, Snežana, Duduk, Natasa, Stanković, Slavica, "The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain" in Genetika, 53, no. 2 (2021):641-649,
https://doi.org/10.2298/GENSR2102641S . .

Germination and the initial seedling growth of lettuce, celeriac and wheat cultivars after micronutrient and a biological application pre-sowing seed treatment

Poštić, Dobrivoj; Štrbanović, Ratibor; Tabaković, Marijenka; Popović, Tatjana; Ćirić, Ana; Banjac, Nevena; Trkulja, Nenad; Stanisavljević, Rade

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Štrbanović, Ratibor
AU  - Tabaković, Marijenka
AU  - Popović, Tatjana
AU  - Ćirić, Ana
AU  - Banjac, Nevena
AU  - Trkulja, Nenad
AU  - Stanisavljević, Rade
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/829
AB  - Seed treatments with zinc, boron, biostimulant Coveron and MIX (zinc + boron + Coveron) were applied to three lettuce and three celeriac cultivars. Seeds of three wheat cultivars were treated under laboratory conditions with Trichoderma harzianum and eight Bacillus spp. Seed germination, seedling growth, and the presence of the following pathogens were determined: Fusarium sp., Al-ternaria sp., Penicillium sp., and Mucor sp. The Coveron treatment was the most effective on lettuce seeds tested in the germination cabinet. Seed germination was higher by 4% than in the control. Alternatively, germination of seeds treated with boron in the greenhouse was higher by 12% than in the control. The Coveron treatment had the highest effect on the shoot length, which was greater by 0.7 and 2.1 cm in the germination cabinet and the greenhouse, respectively. This treatment was also the most effective on the root length. Zn, B, and MIX treatments increased celeriac seed germination by 14% in the germination cabinet. The Zn treatment was the most efficient on seeds tested in the greenhouse. The germination was higher by 15%. A significant cultivar × treatment interaction was determined in both observed species under both conditions. The maximum effect on wheat seed germination (8%) was achieved with the T. harzianum treatment in the Salazar cultivar. A significant interdependence (p ≤ 0.01 to p ≤ 0.001) was established between seed germination and the seedling growth. The interrelationship between seed germination and pathogens of all cultivars was negative.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Plants
T1  - Germination and the initial seedling growth of lettuce, celeriac and wheat cultivars after micronutrient and a biological application pre-sowing seed treatment
VL  - 10
IS  - 9
SP  - 1913
DO  - 10.3390/plants10091913
ER  - 
@article{
author = "Poštić, Dobrivoj and Štrbanović, Ratibor and Tabaković, Marijenka and Popović, Tatjana and Ćirić, Ana and Banjac, Nevena and Trkulja, Nenad and Stanisavljević, Rade",
year = "2021",
abstract = "Seed treatments with zinc, boron, biostimulant Coveron and MIX (zinc + boron + Coveron) were applied to three lettuce and three celeriac cultivars. Seeds of three wheat cultivars were treated under laboratory conditions with Trichoderma harzianum and eight Bacillus spp. Seed germination, seedling growth, and the presence of the following pathogens were determined: Fusarium sp., Al-ternaria sp., Penicillium sp., and Mucor sp. The Coveron treatment was the most effective on lettuce seeds tested in the germination cabinet. Seed germination was higher by 4% than in the control. Alternatively, germination of seeds treated with boron in the greenhouse was higher by 12% than in the control. The Coveron treatment had the highest effect on the shoot length, which was greater by 0.7 and 2.1 cm in the germination cabinet and the greenhouse, respectively. This treatment was also the most effective on the root length. Zn, B, and MIX treatments increased celeriac seed germination by 14% in the germination cabinet. The Zn treatment was the most efficient on seeds tested in the greenhouse. The germination was higher by 15%. A significant cultivar × treatment interaction was determined in both observed species under both conditions. The maximum effect on wheat seed germination (8%) was achieved with the T. harzianum treatment in the Salazar cultivar. A significant interdependence (p ≤ 0.01 to p ≤ 0.001) was established between seed germination and the seedling growth. The interrelationship between seed germination and pathogens of all cultivars was negative.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Plants",
title = "Germination and the initial seedling growth of lettuce, celeriac and wheat cultivars after micronutrient and a biological application pre-sowing seed treatment",
volume = "10",
number = "9",
pages = "1913",
doi = "10.3390/plants10091913"
}
Poštić, D., Štrbanović, R., Tabaković, M., Popović, T., Ćirić, A., Banjac, N., Trkulja, N.,& Stanisavljević, R.. (2021). Germination and the initial seedling growth of lettuce, celeriac and wheat cultivars after micronutrient and a biological application pre-sowing seed treatment. in Plants
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 10(9), 1913.
https://doi.org/10.3390/plants10091913
Poštić D, Štrbanović R, Tabaković M, Popović T, Ćirić A, Banjac N, Trkulja N, Stanisavljević R. Germination and the initial seedling growth of lettuce, celeriac and wheat cultivars after micronutrient and a biological application pre-sowing seed treatment. in Plants. 2021;10(9):1913.
doi:10.3390/plants10091913 .
Poštić, Dobrivoj, Štrbanović, Ratibor, Tabaković, Marijenka, Popović, Tatjana, Ćirić, Ana, Banjac, Nevena, Trkulja, Nenad, Stanisavljević, Rade, "Germination and the initial seedling growth of lettuce, celeriac and wheat cultivars after micronutrient and a biological application pre-sowing seed treatment" in Plants, 10, no. 9 (2021):1913,
https://doi.org/10.3390/plants10091913 . .

Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit

Kravić, Natalija; Babić, Vojka; Vukadinović, Jelena; Ristić, Danijela; Dragičević, Vesna; Mladenović Drinić, Snežana; Anđelković, Violeta

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Vukadinović, Jelena
AU  - Ristić, Danijela
AU  - Dragičević, Vesna
AU  - Mladenović Drinić, Snežana
AU  - Anđelković, Violeta
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/828
AB  - Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biology
T1  - Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit
VL  - 10
IS  - 8
SP  - 694
DO  - 10.3390/biology10080694
ER  - 
@article{
author = "Kravić, Natalija and Babić, Vojka and Vukadinović, Jelena and Ristić, Danijela and Dragičević, Vesna and Mladenović Drinić, Snežana and Anđelković, Violeta",
year = "2021",
abstract = "Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biology",
title = "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit",
volume = "10",
number = "8",
pages = "694",
doi = "10.3390/biology10080694"
}
Kravić, N., Babić, V., Vukadinović, J., Ristić, D., Dragičević, V., Mladenović Drinić, S.,& Anđelković, V.. (2021). Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 694.
https://doi.org/10.3390/biology10080694
Kravić N, Babić V, Vukadinović J, Ristić D, Dragičević V, Mladenović Drinić S, Anđelković V. Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology. 2021;10(8):694.
doi:10.3390/biology10080694 .
Kravić, Natalija, Babić, Vojka, Vukadinović, Jelena, Ristić, Danijela, Dragičević, Vesna, Mladenović Drinić, Snežana, Anđelković, Violeta, "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit" in Biology, 10, no. 8 (2021):694,
https://doi.org/10.3390/biology10080694 . .
2

Diversity assessment of the montenegrin maize landrace gene pool maintained in two gene banks

Babić, Vojka; Anđelković, Violeta; Jovovic, Zoran; Babić, Milosav; Vasić, Vladimir; Kravić, Natalija

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Jovovic, Zoran
AU  - Babić, Milosav
AU  - Vasić, Vladimir
AU  - Kravić, Natalija
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/827
AB  - Due to the loss of agro-biodiversity, there is a strong effort to find apparent and efficient
mechanisms for the conservation and sustainable use of genetic diversity. A joint monitoring of the
diversity and collections structure of the Montenegrin maize landraces conserved in the Serbian
(MRIZPGB) and Montenegrin (MGB) gene banks has been conducted in order to improve the
composition of the collections and to identify and eliminate possible redundancy. Based on a separate
analysis of white- and yellow-orange maize landraces, it can be concluded that the diversity and
evolution of distinct maize landraces grown and collected in Montenegro have been simultaneously
shaped by both environmental (i.e., natural selection) and socially driven factors (farmers’ selection,
migration and colonization processes of the human population). Although it has been determined
that the authenticity and variability of the Montenegrin maize landraces gene pool have largely
been preserved in the MRIZPGB collection, a significant amount of redundancy was observed. The
obtained results will contribute to the cost-efficient conservation of the maize gene pool in the
Montenegrin and Serbian gene banks. The recognized and well-preserved original variability of the
MRIZPGB and MGB Montenegrin gene pool represents a valuable source for pre-breeding activities
on broadening the white and flint maize breeding programmes under temperate conditions.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Plants
T1  - Diversity assessment of the montenegrin maize landrace gene pool maintained in two gene banks
VL  - 10
IS  - 8
SP  - 1503
DO  - 10.3390/plants10081503
ER  - 
@article{
author = "Babić, Vojka and Anđelković, Violeta and Jovovic, Zoran and Babić, Milosav and Vasić, Vladimir and Kravić, Natalija",
year = "2021",
abstract = "Due to the loss of agro-biodiversity, there is a strong effort to find apparent and efficient
mechanisms for the conservation and sustainable use of genetic diversity. A joint monitoring of the
diversity and collections structure of the Montenegrin maize landraces conserved in the Serbian
(MRIZPGB) and Montenegrin (MGB) gene banks has been conducted in order to improve the
composition of the collections and to identify and eliminate possible redundancy. Based on a separate
analysis of white- and yellow-orange maize landraces, it can be concluded that the diversity and
evolution of distinct maize landraces grown and collected in Montenegro have been simultaneously
shaped by both environmental (i.e., natural selection) and socially driven factors (farmers’ selection,
migration and colonization processes of the human population). Although it has been determined
that the authenticity and variability of the Montenegrin maize landraces gene pool have largely
been preserved in the MRIZPGB collection, a significant amount of redundancy was observed. The
obtained results will contribute to the cost-efficient conservation of the maize gene pool in the
Montenegrin and Serbian gene banks. The recognized and well-preserved original variability of the
MRIZPGB and MGB Montenegrin gene pool represents a valuable source for pre-breeding activities
on broadening the white and flint maize breeding programmes under temperate conditions.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Plants",
title = "Diversity assessment of the montenegrin maize landrace gene pool maintained in two gene banks",
volume = "10",
number = "8",
pages = "1503",
doi = "10.3390/plants10081503"
}
Babić, V., Anđelković, V., Jovovic, Z., Babić, M., Vasić, V.,& Kravić, N.. (2021). Diversity assessment of the montenegrin maize landrace gene pool maintained in two gene banks. in Plants
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 1503.
https://doi.org/10.3390/plants10081503
Babić V, Anđelković V, Jovovic Z, Babić M, Vasić V, Kravić N. Diversity assessment of the montenegrin maize landrace gene pool maintained in two gene banks. in Plants. 2021;10(8):1503.
doi:10.3390/plants10081503 .
Babić, Vojka, Anđelković, Violeta, Jovovic, Zoran, Babić, Milosav, Vasić, Vladimir, Kravić, Natalija, "Diversity assessment of the montenegrin maize landrace gene pool maintained in two gene banks" in Plants, 10, no. 8 (2021):1503,
https://doi.org/10.3390/plants10081503 . .
1

High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food

Radenović, Čedomir; Delić, Nenad; Radosavljević, Milica; Jovanović, Života; Sečanski, Mile; Popović, Aleksandar; Crevar, Miloš; Radosavljević, Nebojša

(Beograd : Univerzitet odbrane u Beogradu, Vojna akademija, 2021)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Delić, Nenad
AU  - Radosavljević, Milica
AU  - Jovanović, Života
AU  - Sečanski, Mile
AU  - Popović, Aleksandar
AU  - Crevar, Miloš
AU  - Radosavljević, Nebojša
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/826
AB  - Introduction/purpose: This paper presents the results of several different research studies. The inbred lines ZPPL 146 and ZPPL 159 and the maize hybrids ZP 633, ZP 735, and ZP 737 are primarily intended for human and livestock nutrition. Their selection took about four decades. Methods: Spectral bands were registered using the method of resonant Raman spectroscopy of the leaves of inbred maize lines. These spectral bands indicate the conformational characteristics of not only carotenoid molecules but also other compounds (phosphate, gluten, and amide III) in the leaf. Results: A systematic examination of the inbred lines ZPPL 146 and ZPPL 159 and their maize hybrids ZP 633, ZP 735, and ZP 737 was performed in this paper. It was stated that the new inbred lines of corn, i.e. ZPPL 146 and ZPPL 159, are rich in carotenoids and yellow pigments. These lines also have significant quantities of other valuable bioactive compounds and good physical characteristics. The lines have an upright position of the top leaves and belong to the group of maize lines with significant characteristics of the photosynthetic model. They are resistant to high temperatures and are drought tolerant. Conclusion: This paper presents the relevant properties, characteristics and parameters of the new studied inbred maize lines that can be used in selection processes in the future. High-yielding and high-quality maize hybrids, i.e. ZP 633, ZP 735, and ZP 737, have been created from the mentioned inbred maize lines. They are recognizable by their qualities. The hybrid ZP633 is especially noteworthy for human consumption (children and the elderly). Further, from the agronomic-veterinary point of view, it is confirmed that the hybrids ZP 735 and ZP 737 are the most suitable for livestock feeding with the programmed use of corn silage. The relevant agronomic, morphological and nutritional properties of the maize hybrids ZP 633, ZP 735, and ZP 737 are also presented in this paper. The results regarding the grain structure and yield height for grain and silage for the hybrids ZP 677 and ZP 684, produced in Serbia and the countries of Southeastern Europe, are also given.
PB  - Beograd : Univerzitet odbrane u Beogradu, Vojna akademija
T2  - Vojnotehnički glasnik
T1  - High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food
VL  - 69
IS  - 1
SP  - 114
EP  - 147
DO  - 10.5937/vojteh69-29512
ER  - 
@article{
author = "Radenović, Čedomir and Delić, Nenad and Radosavljević, Milica and Jovanović, Života and Sečanski, Mile and Popović, Aleksandar and Crevar, Miloš and Radosavljević, Nebojša",
year = "2021",
abstract = "Introduction/purpose: This paper presents the results of several different research studies. The inbred lines ZPPL 146 and ZPPL 159 and the maize hybrids ZP 633, ZP 735, and ZP 737 are primarily intended for human and livestock nutrition. Their selection took about four decades. Methods: Spectral bands were registered using the method of resonant Raman spectroscopy of the leaves of inbred maize lines. These spectral bands indicate the conformational characteristics of not only carotenoid molecules but also other compounds (phosphate, gluten, and amide III) in the leaf. Results: A systematic examination of the inbred lines ZPPL 146 and ZPPL 159 and their maize hybrids ZP 633, ZP 735, and ZP 737 was performed in this paper. It was stated that the new inbred lines of corn, i.e. ZPPL 146 and ZPPL 159, are rich in carotenoids and yellow pigments. These lines also have significant quantities of other valuable bioactive compounds and good physical characteristics. The lines have an upright position of the top leaves and belong to the group of maize lines with significant characteristics of the photosynthetic model. They are resistant to high temperatures and are drought tolerant. Conclusion: This paper presents the relevant properties, characteristics and parameters of the new studied inbred maize lines that can be used in selection processes in the future. High-yielding and high-quality maize hybrids, i.e. ZP 633, ZP 735, and ZP 737, have been created from the mentioned inbred maize lines. They are recognizable by their qualities. The hybrid ZP633 is especially noteworthy for human consumption (children and the elderly). Further, from the agronomic-veterinary point of view, it is confirmed that the hybrids ZP 735 and ZP 737 are the most suitable for livestock feeding with the programmed use of corn silage. The relevant agronomic, morphological and nutritional properties of the maize hybrids ZP 633, ZP 735, and ZP 737 are also presented in this paper. The results regarding the grain structure and yield height for grain and silage for the hybrids ZP 677 and ZP 684, produced in Serbia and the countries of Southeastern Europe, are also given.",
publisher = "Beograd : Univerzitet odbrane u Beogradu, Vojna akademija",
journal = "Vojnotehnički glasnik",
title = "High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food",
volume = "69",
number = "1",
pages = "114-147",
doi = "10.5937/vojteh69-29512"
}
Radenović, Č., Delić, N., Radosavljević, M., Jovanović, Ž., Sečanski, M., Popović, A., Crevar, M.,& Radosavljević, N.. (2021). High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food. in Vojnotehnički glasnik
Beograd : Univerzitet odbrane u Beogradu, Vojna akademija., 69(1), 114-147.
https://doi.org/10.5937/vojteh69-29512
Radenović Č, Delić N, Radosavljević M, Jovanović Ž, Sečanski M, Popović A, Crevar M, Radosavljević N. High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food. in Vojnotehnički glasnik. 2021;69(1):114-147.
doi:10.5937/vojteh69-29512 .
Radenović, Čedomir, Delić, Nenad, Radosavljević, Milica, Jovanović, Života, Sečanski, Mile, Popović, Aleksandar, Crevar, Miloš, Radosavljević, Nebojša, "High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food" in Vojnotehnički glasnik, 69, no. 1 (2021):114-147,
https://doi.org/10.5937/vojteh69-29512 . .

Effects of pre-sowing seed treatments for improving germination and the growth of pepper and tomato seedlings

Štrbanović, Ratibor; Poštić, Dobrivoj; Tabaković, Marijenka; Knežević, Jasmina; Živanović, Ljubiša; Stanisavljević, Rade

(Lublin : Wydawnictwo Akademii Rolniczej, 2021)

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Poštić, Dobrivoj
AU  - Tabaković, Marijenka
AU  - Knežević, Jasmina
AU  - Živanović, Ljubiša
AU  - Stanisavljević, Rade
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/821
AB  - The aim of this study was to establish the effect of different seed treatments on germination
,
 and the growth 
of  the  embryonic  stem  and  the  radicle  of  tomato  and  pepper  varieties.  Four  treatments  were  used  in  the  
study: MIX [(Coveron + zinc (Zn 0.5%) + boron (B 0.025%)]; Coveron; zinc (ZnSO
4
, Zn 0.5%) and boron 
(B 0.025%). The treatments were applied on seeds of following four pepper varieties: Šorokšari, Somborka, 
Kraljica, and Mirtima and three tomato varieties: Rio Grande, Saint Pierre, and Tomato apple of Novi Sad 
(Novosadski jabučar). Germination and the growth increase of both the embryonic stem (cm) and the radicle 
(cm) were observed in the germination cabinets and pots containing soil in two laboratories – locations. Af-
ter the treatment applied to the pepper seeds and testing in the laboratory germination cabinet the following 
was established: i) the maximum increase in germination of 90% was when the MIX and Zn treatment was 
applied to seeds, ii) the growth increase of embryonic stems of 2.7 cm was recorded when the MIX treatment 
was applied, iii) the growth increase of radicles of 1.7 cm was gained when the Coveron and MIX treatment 
was applied. Tests performed in pots showed that Coveron was the most efficient treatment. Treatments on 
tomato seeds during the seed testing in the germination cabinet provided: vi) the germination increase of 13% 
with the MIX treatment, vii) the growth increase of the embryonic stem of 2.6 cm with the same treatment, 
vii) the growth increase of 1.7 cm of radicles. Coveron was the most efficient treatment in tests in pots.
PB  - Lublin : Wydawnictwo Akademii Rolniczej
T2  - Acta Scientiarum Polonorum, Hortorum Cultus
T1  - Effects of pre-sowing seed treatments for improving germination and the growth of pepper and tomato seedlings
VL  - 20
IS  - 1
SP  - 101
EP  - 109
DO  - 10.24326/asphc.2021.1.9
ER  - 
@article{
author = "Štrbanović, Ratibor and Poštić, Dobrivoj and Tabaković, Marijenka and Knežević, Jasmina and Živanović, Ljubiša and Stanisavljević, Rade",
year = "2021",
abstract = "The aim of this study was to establish the effect of different seed treatments on germination
,
 and the growth 
of  the  embryonic  stem  and  the  radicle  of  tomato  and  pepper  varieties.  Four  treatments  were  used  in  the  
study: MIX [(Coveron + zinc (Zn 0.5%) + boron (B 0.025%)]; Coveron; zinc (ZnSO
4
, Zn 0.5%) and boron 
(B 0.025%). The treatments were applied on seeds of following four pepper varieties: Šorokšari, Somborka, 
Kraljica, and Mirtima and three tomato varieties: Rio Grande, Saint Pierre, and Tomato apple of Novi Sad 
(Novosadski jabučar). Germination and the growth increase of both the embryonic stem (cm) and the radicle 
(cm) were observed in the germination cabinets and pots containing soil in two laboratories – locations. Af-
ter the treatment applied to the pepper seeds and testing in the laboratory germination cabinet the following 
was established: i) the maximum increase in germination of 90% was when the MIX and Zn treatment was 
applied to seeds, ii) the growth increase of embryonic stems of 2.7 cm was recorded when the MIX treatment 
was applied, iii) the growth increase of radicles of 1.7 cm was gained when the Coveron and MIX treatment 
was applied. Tests performed in pots showed that Coveron was the most efficient treatment. Treatments on 
tomato seeds during the seed testing in the germination cabinet provided: vi) the germination increase of 13% 
with the MIX treatment, vii) the growth increase of the embryonic stem of 2.6 cm with the same treatment, 
vii) the growth increase of 1.7 cm of radicles. Coveron was the most efficient treatment in tests in pots.",
publisher = "Lublin : Wydawnictwo Akademii Rolniczej",
journal = "Acta Scientiarum Polonorum, Hortorum Cultus",
title = "Effects of pre-sowing seed treatments for improving germination and the growth of pepper and tomato seedlings",
volume = "20",
number = "1",
pages = "101-109",
doi = "10.24326/asphc.2021.1.9"
}
Štrbanović, R., Poštić, D., Tabaković, M., Knežević, J., Živanović, L.,& Stanisavljević, R.. (2021). Effects of pre-sowing seed treatments for improving germination and the growth of pepper and tomato seedlings. in Acta Scientiarum Polonorum, Hortorum Cultus
Lublin : Wydawnictwo Akademii Rolniczej., 20(1), 101-109.
https://doi.org/10.24326/asphc.2021.1.9
Štrbanović R, Poštić D, Tabaković M, Knežević J, Živanović L, Stanisavljević R. Effects of pre-sowing seed treatments for improving germination and the growth of pepper and tomato seedlings. in Acta Scientiarum Polonorum, Hortorum Cultus. 2021;20(1):101-109.
doi:10.24326/asphc.2021.1.9 .
Štrbanović, Ratibor, Poštić, Dobrivoj, Tabaković, Marijenka, Knežević, Jasmina, Živanović, Ljubiša, Stanisavljević, Rade, "Effects of pre-sowing seed treatments for improving germination and the growth of pepper and tomato seedlings" in Acta Scientiarum Polonorum, Hortorum Cultus, 20, no. 1 (2021):101-109,
https://doi.org/10.24326/asphc.2021.1.9 . .
1

The impact of diet and fibre fractions on plasma adipocytokine levels in prediabetic adults

Dodevska, Margarita; Šobajić, Slađana; Dragičević, Vesna; Stanković, Ivan; Ivanović, Nevena; Đorđević, Brižita

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Dodevska, Margarita
AU  - Šobajić, Slađana
AU  - Dragičević, Vesna
AU  - Stanković, Ivan
AU  - Ivanović, Nevena
AU  - Đorđević, Brižita
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/822
AB  - The impact of diet and fibre fractions on adipocytokines in obese subjects with a risk of diabetes has not been investigated in detail yet. The purpose of the study is to evaluate the effects of a 12-month lifestyle intervention with different fibre profiles (resistant starch (RS)—rich fibre, or ordinary food fibre profiles) on adipocytokine levels. Fifty participants are divided into two groups (RS group and Fibre group). The groups differ only in the percentage of the recommended level of the RS consumed as a fraction of the same total fibre amount. The applied dietary intervention includes intake of 7531 KJ/daywith a total fibre portion of 25–35 g/dayfor both groups that includes 15 g/day of RS for the RS group only. The levels of leptin, adiponectin, apelin, resistin, tumor necrosis factor (TNF)-alpha and C-reactive protein (CRP) are measured, and their relationship to anthropometric and biochemical parameters is estimated. Along with significant body weight loss, only leptin is significantly reduced by 13% in the RS group while in the Fibre group, apelin levels are significant (−21%). Polynomial regression shows a negative correlation between RS intake and adiponectin (R2 = 0.145) and resistin level (R2 = 0.461) in the RS group. This study indicates the possibility that fibre fractions differently influence the outcome of lifestyle interventions, as well as their adipocytokine levels, in obese prediabetic adults.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Nutrients
T1  - The impact of diet and fibre fractions on plasma adipocytokine levels in prediabetic adults
VL  - 13
IS  - 2
SP  - 487
DO  - 10.3390/nu13020487
ER  - 
@article{
author = "Dodevska, Margarita and Šobajić, Slađana and Dragičević, Vesna and Stanković, Ivan and Ivanović, Nevena and Đorđević, Brižita",
year = "2021",
abstract = "The impact of diet and fibre fractions on adipocytokines in obese subjects with a risk of diabetes has not been investigated in detail yet. The purpose of the study is to evaluate the effects of a 12-month lifestyle intervention with different fibre profiles (resistant starch (RS)—rich fibre, or ordinary food fibre profiles) on adipocytokine levels. Fifty participants are divided into two groups (RS group and Fibre group). The groups differ only in the percentage of the recommended level of the RS consumed as a fraction of the same total fibre amount. The applied dietary intervention includes intake of 7531 KJ/daywith a total fibre portion of 25–35 g/dayfor both groups that includes 15 g/day of RS for the RS group only. The levels of leptin, adiponectin, apelin, resistin, tumor necrosis factor (TNF)-alpha and C-reactive protein (CRP) are measured, and their relationship to anthropometric and biochemical parameters is estimated. Along with significant body weight loss, only leptin is significantly reduced by 13% in the RS group while in the Fibre group, apelin levels are significant (−21%). Polynomial regression shows a negative correlation between RS intake and adiponectin (R2 = 0.145) and resistin level (R2 = 0.461) in the RS group. This study indicates the possibility that fibre fractions differently influence the outcome of lifestyle interventions, as well as their adipocytokine levels, in obese prediabetic adults.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Nutrients",
title = "The impact of diet and fibre fractions on plasma adipocytokine levels in prediabetic adults",
volume = "13",
number = "2",
pages = "487",
doi = "10.3390/nu13020487"
}
Dodevska, M., Šobajić, S., Dragičević, V., Stanković, I., Ivanović, N.,& Đorđević, B.. (2021). The impact of diet and fibre fractions on plasma adipocytokine levels in prediabetic adults. in Nutrients
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 13(2), 487.
https://doi.org/10.3390/nu13020487
Dodevska M, Šobajić S, Dragičević V, Stanković I, Ivanović N, Đorđević B. The impact of diet and fibre fractions on plasma adipocytokine levels in prediabetic adults. in Nutrients. 2021;13(2):487.
doi:10.3390/nu13020487 .
Dodevska, Margarita, Šobajić, Slađana, Dragičević, Vesna, Stanković, Ivan, Ivanović, Nevena, Đorđević, Brižita, "The impact of diet and fibre fractions on plasma adipocytokine levels in prediabetic adults" in Nutrients, 13, no. 2 (2021):487,
https://doi.org/10.3390/nu13020487 . .

Harvest time effect on quantitative and qualitative parameters of forage maize

Mandić, Violeta; Bijelić, Zorica; Krnjaja, Vesna; Đorđević, Snežana; Brankov, Milan; Mićić, Nenad; Stanojković, Aleksandar

(Lahore : Pakistan Agricultural Scientists Forum, 2021)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Đorđević, Snežana
AU  - Brankov, Milan
AU  - Mićić, Nenad
AU  - Stanojković, Aleksandar
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/811
AB  - Maize silage is source of palatable and high-energy forage for ruminants. Therefore, production of high quality forage maize represents an essential strategy for stable production of milk and meat on livestock farms. This study examined the effect of harvest date (early dent, at half milk line, at three quarters milk line and black layer) on the quantitative and qualitative parameters of whole maize plant under contrasting climatic conditions in the Srem - Serbia. A 2 × 4 factorial (two years and the four cutting times) randomized blocks design was used, with three replications. The plant height, stem diameter, number of leaves per plant, forage yield, dry matter yield and dry matter content were higher in 2014 probably due to favorable weather conditions. With the delay of the harvest the forage yield, crude protein content, acid detergent fiber (ADF) and neutral detergent fiber (NDF) decreased, and dry matter yield, dry matter content and ear percentage increased. Sufficiently high dry matter content and ear percentage were achieved at the third harvest. However, the delay of harvested time reduces the quality parameters of the biomass, but this loss in the entire plant is moderated by grain filling.
PB  - Lahore : Pakistan Agricultural Scientists Forum
T2  - Journal of Animal and Plant Sciences
T1  - Harvest time effect on quantitative and qualitative parameters of forage maize
VL  - 31
IS  - 1
SP  - 103
EP  - 107
DO  - 10.36899/JAPS.2021.1.0198
ER  - 
@article{
author = "Mandić, Violeta and Bijelić, Zorica and Krnjaja, Vesna and Đorđević, Snežana and Brankov, Milan and Mićić, Nenad and Stanojković, Aleksandar",
year = "2021",
abstract = "Maize silage is source of palatable and high-energy forage for ruminants. Therefore, production of high quality forage maize represents an essential strategy for stable production of milk and meat on livestock farms. This study examined the effect of harvest date (early dent, at half milk line, at three quarters milk line and black layer) on the quantitative and qualitative parameters of whole maize plant under contrasting climatic conditions in the Srem - Serbia. A 2 × 4 factorial (two years and the four cutting times) randomized blocks design was used, with three replications. The plant height, stem diameter, number of leaves per plant, forage yield, dry matter yield and dry matter content were higher in 2014 probably due to favorable weather conditions. With the delay of the harvest the forage yield, crude protein content, acid detergent fiber (ADF) and neutral detergent fiber (NDF) decreased, and dry matter yield, dry matter content and ear percentage increased. Sufficiently high dry matter content and ear percentage were achieved at the third harvest. However, the delay of harvested time reduces the quality parameters of the biomass, but this loss in the entire plant is moderated by grain filling.",
publisher = "Lahore : Pakistan Agricultural Scientists Forum",
journal = "Journal of Animal and Plant Sciences",
title = "Harvest time effect on quantitative and qualitative parameters of forage maize",
volume = "31",
number = "1",
pages = "103-107",
doi = "10.36899/JAPS.2021.1.0198"
}
Mandić, V., Bijelić, Z., Krnjaja, V., Đorđević, S., Brankov, M., Mićić, N.,& Stanojković, A.. (2021). Harvest time effect on quantitative and qualitative parameters of forage maize. in Journal of Animal and Plant Sciences
Lahore : Pakistan Agricultural Scientists Forum., 31(1), 103-107.
https://doi.org/10.36899/JAPS.2021.1.0198
Mandić V, Bijelić Z, Krnjaja V, Đorđević S, Brankov M, Mićić N, Stanojković A. Harvest time effect on quantitative and qualitative parameters of forage maize. in Journal of Animal and Plant Sciences. 2021;31(1):103-107.
doi:10.36899/JAPS.2021.1.0198 .
Mandić, Violeta, Bijelić, Zorica, Krnjaja, Vesna, Đorđević, Snežana, Brankov, Milan, Mićić, Nenad, Stanojković, Aleksandar, "Harvest time effect on quantitative and qualitative parameters of forage maize" in Journal of Animal and Plant Sciences, 31, no. 1 (2021):103-107,
https://doi.org/10.36899/JAPS.2021.1.0198 . .

Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses

Stanisavljević, Rade; Poštić, Dobrivoj; Štrbanović, Ratibor; Tabaković, Marijenka; Jovanović, Snežana; Milenković, Jasmina; Đokić, Dragoslav; Terzić, Dragan

(Valle del Cauca : Centro Internacional de Agricultura Tropical (CIAT), 2020)

TY  - JOUR
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Štrbanović, Ratibor
AU  - Tabaković, Marijenka
AU  - Jovanović, Snežana
AU  - Milenković, Jasmina
AU  - Đokić, Dragoslav
AU  - Terzić, Dragan
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/824
AB  - Tests of seed germination, seed dormancy and seedling growth were performed on 0-, 6-, 20- and 30-months-old seed lots of Festulolium in comparison with Italian ryegrass (Lolium multiflorum) and meadow fescue (Festuca pratensis). Tests were performed on seeds harvested in 2 different years (2014 and 2015) resulting in no major difference between the years. Seed storage affected seed viability and dormancy and seedling growth in all 3 grasses. The maximum germination of Festulolium seeds was achieved 6 months after harvest (95% normal seedlings); germination decreased significantly thereafter. While maximum germination of L. multiflorum and F. pratensis seeds was also achieved following storage for 6 months, these germination rates (93 and 90%, respectively) were retained until at least 20 months in storage. After storage for 30 months, seed germination of Festulolium, L. multiflorum and F. pratensis had declined to 72, 79 and 83%, respectively. High germination in all species was associated with higher rates of seedling growth. In an artificial seed ageing test, a temperature of 41 °C (during 48 and 72 hours) was found to effectively rank seed lots for germination performance in all 3 grasses. This test seems to have application for use in the seed trade to identify seed lots which could deteriorate more rapidly in storage. Further studies are needed to verify this hypothesis.
AB  - Se realizaron pruebas de germinación y de crecimiento de plántulas provenientes de lotes de semillas de Festulolium almacenadas durante 0, 6, 20 y 30 meses, en comparación con raigrás italiano (Lolium multiflorum) y festuca de pradera (Festuca pratensis). En lotes de semillas cosechadas en 2014 y 2015 no se encontraron diferencias entre los años. El almacenamiento afectó la viabilidad y la latencia de las semillas y el crecimiento de las plántulas en las tres especies. La germinación máxima de las semillas de Festulolium se presentó 6 meses después de la cosecha (95% de plántulas normales), a partir de los cuales disminuyó significativamente. También las semillas de L. multiflorum y F. pratensis presentaron máxima germinación después de 6 meses (93 y 90%, respectivamente); estas tasas, sin embargo, se mantuvieron hasta al menos 20 meses de almacenamiento. Después de 30 meses, la germinación de las semillas disminuyó a 72, 79 y 83% para Festulolium, raigrás y festuca, respectivamente. La alta germinación en todas las especies se asoció con mayores tasas de crecimiento de plántulas. En una prueba rápida de envejecimiento artificial de semillas (temperatura de 41 °C durante 48 y 72 horas) fue posible predecir el comportamiento de germinación de las semillas de las tres especies. Esta prueba parece tener aplicación en el comercio para identificar lotes de semillas que podrían deteriorarse más rápidamente durante el almacenamiento. Se necesitan más estudios para verificar esta hipótesis.
PB  - Valle del Cauca : Centro Internacional de Agricultura Tropical (CIAT)
T2  - Tropical Grasslands-Forrajes Tropicales
T1  - Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses
T1  - Efecto del almacenamiento de la semilla de Festulolium y especies relacionadas en su germinación y la calidad de plántulas
VL  - 8
IS  - 2
SP  - 125
EP  - 132
DO  - 10.17138/TGFT(8)125-132
ER  - 
@article{
author = "Stanisavljević, Rade and Poštić, Dobrivoj and Štrbanović, Ratibor and Tabaković, Marijenka and Jovanović, Snežana and Milenković, Jasmina and Đokić, Dragoslav and Terzić, Dragan",
year = "2020",
abstract = "Tests of seed germination, seed dormancy and seedling growth were performed on 0-, 6-, 20- and 30-months-old seed lots of Festulolium in comparison with Italian ryegrass (Lolium multiflorum) and meadow fescue (Festuca pratensis). Tests were performed on seeds harvested in 2 different years (2014 and 2015) resulting in no major difference between the years. Seed storage affected seed viability and dormancy and seedling growth in all 3 grasses. The maximum germination of Festulolium seeds was achieved 6 months after harvest (95% normal seedlings); germination decreased significantly thereafter. While maximum germination of L. multiflorum and F. pratensis seeds was also achieved following storage for 6 months, these germination rates (93 and 90%, respectively) were retained until at least 20 months in storage. After storage for 30 months, seed germination of Festulolium, L. multiflorum and F. pratensis had declined to 72, 79 and 83%, respectively. High germination in all species was associated with higher rates of seedling growth. In an artificial seed ageing test, a temperature of 41 °C (during 48 and 72 hours) was found to effectively rank seed lots for germination performance in all 3 grasses. This test seems to have application for use in the seed trade to identify seed lots which could deteriorate more rapidly in storage. Further studies are needed to verify this hypothesis., Se realizaron pruebas de germinación y de crecimiento de plántulas provenientes de lotes de semillas de Festulolium almacenadas durante 0, 6, 20 y 30 meses, en comparación con raigrás italiano (Lolium multiflorum) y festuca de pradera (Festuca pratensis). En lotes de semillas cosechadas en 2014 y 2015 no se encontraron diferencias entre los años. El almacenamiento afectó la viabilidad y la latencia de las semillas y el crecimiento de las plántulas en las tres especies. La germinación máxima de las semillas de Festulolium se presentó 6 meses después de la cosecha (95% de plántulas normales), a partir de los cuales disminuyó significativamente. También las semillas de L. multiflorum y F. pratensis presentaron máxima germinación después de 6 meses (93 y 90%, respectivamente); estas tasas, sin embargo, se mantuvieron hasta al menos 20 meses de almacenamiento. Después de 30 meses, la germinación de las semillas disminuyó a 72, 79 y 83% para Festulolium, raigrás y festuca, respectivamente. La alta germinación en todas las especies se asoció con mayores tasas de crecimiento de plántulas. En una prueba rápida de envejecimiento artificial de semillas (temperatura de 41 °C durante 48 y 72 horas) fue posible predecir el comportamiento de germinación de las semillas de las tres especies. Esta prueba parece tener aplicación en el comercio para identificar lotes de semillas que podrían deteriorarse más rápidamente durante el almacenamiento. Se necesitan más estudios para verificar esta hipótesis.",
publisher = "Valle del Cauca : Centro Internacional de Agricultura Tropical (CIAT)",
journal = "Tropical Grasslands-Forrajes Tropicales",
title = "Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses, Efecto del almacenamiento de la semilla de Festulolium y especies relacionadas en su germinación y la calidad de plántulas",
volume = "8",
number = "2",
pages = "125-132",
doi = "10.17138/TGFT(8)125-132"
}
Stanisavljević, R., Poštić, D., Štrbanović, R., Tabaković, M., Jovanović, S., Milenković, J., Đokić, D.,& Terzić, D.. (2020). Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses. in Tropical Grasslands-Forrajes Tropicales
Valle del Cauca : Centro Internacional de Agricultura Tropical (CIAT)., 8(2), 125-132.
https://doi.org/10.17138/TGFT(8)125-132
Stanisavljević R, Poštić D, Štrbanović R, Tabaković M, Jovanović S, Milenković J, Đokić D, Terzić D. Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses. in Tropical Grasslands-Forrajes Tropicales. 2020;8(2):125-132.
doi:10.17138/TGFT(8)125-132 .
Stanisavljević, Rade, Poštić, Dobrivoj, Štrbanović, Ratibor, Tabaković, Marijenka, Jovanović, Snežana, Milenković, Jasmina, Đokić, Dragoslav, Terzić, Dragan, "Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses" in Tropical Grasslands-Forrajes Tropicales, 8, no. 2 (2020):125-132,
https://doi.org/10.17138/TGFT(8)125-132 . .
3

Phylogeography of some european populations of the sugar beet cyst nematode

Oro, Violeta; Tabaković, Marijenka

(Russian Society of Nematologists, 2020)

TY  - JOUR
AU  - Oro, Violeta
AU  - Tabaković, Marijenka
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/823
AB  - Sugar  beet  is  an  important  crop  of  temperate  climates  and  Serbia.  The  paleobotanic  data  suggest the sea beet was grown from ancient times, while the beets with swollen roots were cultivated in the  Middle  Ages  in  Europe.  Phylogeography  of  the  European  populations  of Heterodera  schachtii,  a  nematode parasite on sugar beet, using Maximum Likelihood and Bayesian analyses was studied. Results based on matching the historical data with phylogenetic analyses based on the ITS rRNA region indicate the area across the Dutch-Belgian coastal region as a possible place of origin of the European H. schachtiipopulations. In  addition,  the  dendrograms  reveal  a  clear  distinction  between  the  two  sister  species  (H. schachtii and H. betae) that coexist on the same host.
PB  - Russian Society of Nematologists
T2  - Russian Journal of Nematology
T1  - Phylogeography of some european populations of the sugar beet cyst nematode
VL  - 28
IS  - 2
SP  - 91
EP  - 98
DO  - 10.24411/0869-6918-2020-10009
ER  - 
@article{
author = "Oro, Violeta and Tabaković, Marijenka",
year = "2020",
abstract = "Sugar  beet  is  an  important  crop  of  temperate  climates  and  Serbia.  The  paleobotanic  data  suggest the sea beet was grown from ancient times, while the beets with swollen roots were cultivated in the  Middle  Ages  in  Europe.  Phylogeography  of  the  European  populations  of Heterodera  schachtii,  a  nematode parasite on sugar beet, using Maximum Likelihood and Bayesian analyses was studied. Results based on matching the historical data with phylogenetic analyses based on the ITS rRNA region indicate the area across the Dutch-Belgian coastal region as a possible place of origin of the European H. schachtiipopulations. In  addition,  the  dendrograms  reveal  a  clear  distinction  between  the  two  sister  species  (H. schachtii and H. betae) that coexist on the same host.",
publisher = "Russian Society of Nematologists",
journal = "Russian Journal of Nematology",
title = "Phylogeography of some european populations of the sugar beet cyst nematode",
volume = "28",
number = "2",
pages = "91-98",
doi = "10.24411/0869-6918-2020-10009"
}
Oro, V.,& Tabaković, M.. (2020). Phylogeography of some european populations of the sugar beet cyst nematode. in Russian Journal of Nematology
Russian Society of Nematologists., 28(2), 91-98.
https://doi.org/10.24411/0869-6918-2020-10009
Oro V, Tabaković M. Phylogeography of some european populations of the sugar beet cyst nematode. in Russian Journal of Nematology. 2020;28(2):91-98.
doi:10.24411/0869-6918-2020-10009 .
Oro, Violeta, Tabaković, Marijenka, "Phylogeography of some european populations of the sugar beet cyst nematode" in Russian Journal of Nematology, 28, no. 2 (2020):91-98,
https://doi.org/10.24411/0869-6918-2020-10009 . .

Black soya bean and black chia seeds as a source of nutrients and bioactive compounds with health benefits

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Perić, Vesna

(Novi Sad : Institute of Food Technology, University of Novi Sad, 2020)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Perić, Vesna
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/820
AB  - Recent trends in healthy lifestyle and diet made functional foods rich in
quality nutrients and bioactive compounds with potential health benefits highly sought
after. Some cultivated plants, such as soya and chia can provide a viable source of
nutraceuticals with high fibre, protein, and protective antioxidant potential. The wholegrain flours of black soya beans and black chia seeds were used in this study. Potential
nutritive and health-benefitting properties of these flours were compared by assessing
their chemical composition and antioxidant profile. The content of dietary fibres such
as NDF, ADF, ADL and hemicellulose determined in black chia seed sample was
higher than in black soya soya beans which had higher fibre content. The total protein
content recorded in black soya beans was almost as twice as high (42.26+0.14%) as
chia protein content (25.04+0,20%). Black soya beans had the highest content of water
soluble proteins (29.00+0.13% d.m.) with NSI (nitrogen solubility index) acounting
for as much as 70.96+0.31% of total proteins, while black chia seeds had the highest
content of globulins (14.64+0.07% d.m.) and NSI 58.48+0.27% of total proteins. Both
black soya bean and black chia contained a high amount of total phenolic compounds
(830,66+5.46 and 1201.94+16.29 mg GAE/kg, respectively) and exhibited a
considerable total antioxidant capacity, which makes them good contestants for
functional food ingredients with potential health benefits.
PB  - Novi Sad : Institute of Food Technology, University of Novi Sad
T2  - Food and Feed Research
T1  - Black soya bean and black chia seeds as a source of nutrients and bioactive compounds with health benefits
VL  - 47
IS  - 2
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Perić, Vesna",
year = "2020",
abstract = "Recent trends in healthy lifestyle and diet made functional foods rich in
quality nutrients and bioactive compounds with potential health benefits highly sought
after. Some cultivated plants, such as soya and chia can provide a viable source of
nutraceuticals with high fibre, protein, and protective antioxidant potential. The wholegrain flours of black soya beans and black chia seeds were used in this study. Potential
nutritive and health-benefitting properties of these flours were compared by assessing
their chemical composition and antioxidant profile. The content of dietary fibres such
as NDF, ADF, ADL and hemicellulose determined in black chia seed sample was
higher than in black soya soya beans which had higher fibre content. The total protein
content recorded in black soya beans was almost as twice as high (42.26+0.14%) as
chia protein content (25.04+0,20%). Black soya beans had the highest content of water
soluble proteins (29.00+0.13% d.m.) with NSI (nitrogen solubility index) acounting
for as much as 70.96+0.31% of total proteins, while black chia seeds had the highest
content of globulins (14.64+0.07% d.m.) and NSI 58.48+0.27% of total proteins. Both
black soya bean and black chia contained a high amount of total phenolic compounds
(830,66+5.46 and 1201.94+16.29 mg GAE/kg, respectively) and exhibited a
considerable total antioxidant capacity, which makes them good contestants for
functional food ingredients with potential health benefits.",
publisher = "Novi Sad : Institute of Food Technology, University of Novi Sad",
journal = "Food and Feed Research",
title = "Black soya bean and black chia seeds as a source of nutrients and bioactive compounds with health benefits",
volume = "47",
number = "2"
}
Nikolić, V., Žilić, S., Simić, M.,& Perić, V.. (2020). Black soya bean and black chia seeds as a source of nutrients and bioactive compounds with health benefits. in Food and Feed Research
Novi Sad : Institute of Food Technology, University of Novi Sad., 47(2).
Nikolić V, Žilić S, Simić M, Perić V. Black soya bean and black chia seeds as a source of nutrients and bioactive compounds with health benefits. in Food and Feed Research. 2020;47(2)..
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Perić, Vesna, "Black soya bean and black chia seeds as a source of nutrients and bioactive compounds with health benefits" in Food and Feed Research, 47, no. 2 (2020).

The response of maize lines to foliar fertilizing

Brankov, Milan; Simić, Milena; Dolijanović, Željko; Rajković, Miloš; Mandić, Violeta; Dragičević, Vesna

(Basel : MDPI AG, 2020)

TY  - JOUR
AU  - Brankov, Milan
AU  - Simić, Milena
AU  - Dolijanović, Željko
AU  - Rajković, Miloš
AU  - Mandić, Violeta
AU  - Dragičević, Vesna
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/810
AB  - The objective of this study was to evaluate the impact of two foliar fertilizers applied on five
maize (Zea mays L.) lines. Fertilizers were applied at different growth stages of maize, during three consecutive years (2010–2012) at the experimental field of the Maize Research Institute “Zemun Polje”, Serbia. Maize growth parameters such as fresh matter, height, leaf area and grain yield were recorded. Foliar fertilizer with amino acids (FAA) was more advantageous to maize plants compared to fertilizer containing phosphorus (FP) as a main component. Applied FAA has shown positive effects by increasing fresh matter, leaf area index, and plant height in all three years. In 2012, due to unfavorable meteorological conditions, grain yield and harvest index were very low, compared to the previous two years, although, positive effects on morphological traits were observed 21 days after treatments (DAT), as well as in the anthesis stage. The best results of 30% of grain yield and harvest index increase were recorded in line L1 in 2010 and 2011. The same line had an increase of more than 40% of fresh matter and leaf area on average for all three years. The positive effects that have been noticed in this research could recommend foliar fertilizing with fertilizer containing N in a form of an amino acids complex.
PB  - Basel : MDPI AG
T2  - Agriculture (Switzerland)
T1  - The response of maize lines to foliar fertilizing
VL  - 10
IS  - 9
SP  - 365
DO  - 10.3390/agriculture10090365
ER  - 
@article{
author = "Brankov, Milan and Simić, Milena and Dolijanović, Željko and Rajković, Miloš and Mandić, Violeta and Dragičević, Vesna",
year = "2020",
abstract = "The objective of this study was to evaluate the impact of two foliar fertilizers applied on five
maize (Zea mays L.) lines. Fertilizers were applied at different growth stages of maize, during three consecutive years (2010–2012) at the experimental field of the Maize Research Institute “Zemun Polje”, Serbia. Maize growth parameters such as fresh matter, height, leaf area and grain yield were recorded. Foliar fertilizer with amino acids (FAA) was more advantageous to maize plants compared to fertilizer containing phosphorus (FP) as a main component. Applied FAA has shown positive effects by increasing fresh matter, leaf area index, and plant height in all three years. In 2012, due to unfavorable meteorological conditions, grain yield and harvest index were very low, compared to the previous two years, although, positive effects on morphological traits were observed 21 days after treatments (DAT), as well as in the anthesis stage. The best results of 30% of grain yield and harvest index increase were recorded in line L1 in 2010 and 2011. The same line had an increase of more than 40% of fresh matter and leaf area on average for all three years. The positive effects that have been noticed in this research could recommend foliar fertilizing with fertilizer containing N in a form of an amino acids complex.",
publisher = "Basel : MDPI AG",
journal = "Agriculture (Switzerland)",
title = "The response of maize lines to foliar fertilizing",
volume = "10",
number = "9",
pages = "365",
doi = "10.3390/agriculture10090365"
}
Brankov, M., Simić, M., Dolijanović, Ž., Rajković, M., Mandić, V.,& Dragičević, V.. (2020). The response of maize lines to foliar fertilizing. in Agriculture (Switzerland)
Basel : MDPI AG., 10(9), 365.
https://doi.org/10.3390/agriculture10090365
Brankov M, Simić M, Dolijanović Ž, Rajković M, Mandić V, Dragičević V. The response of maize lines to foliar fertilizing. in Agriculture (Switzerland). 2020;10(9):365.
doi:10.3390/agriculture10090365 .
Brankov, Milan, Simić, Milena, Dolijanović, Željko, Rajković, Miloš, Mandić, Violeta, Dragičević, Vesna, "The response of maize lines to foliar fertilizing" in Agriculture (Switzerland), 10, no. 9 (2020):365,
https://doi.org/10.3390/agriculture10090365 . .
4
3

The contribution of soil tillage and nitrogen rate to the quality of maize grain

Simić, Milena; Dragičević, Vesna; Mladenović Drinić, Snežana; Vukadinović, Jelena; Kresović, Branka; Tabaković, Marijenka; Brankov, Milan

(Basel : MDPI, 2020)

TY  - JOUR
AU  - Simić, Milena
AU  - Dragičević, Vesna
AU  - Mladenović Drinić, Snežana
AU  - Vukadinović, Jelena
AU  - Kresović, Branka
AU  - Tabaković, Marijenka
AU  - Brankov, Milan
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/802
AB  - A soil tillage system adjusted to the soil type and agro-ecological conditions, togetherwith the optimal nutrient supply, is a prerequisite for high maize (Zea mays L.) yield. However,there is little knowledge about the influence of soil tillage and nitrogen (N) rates on maize grainquality. A study was initiated in 1978 in Zemun Polje, Belgrade, Serbia, on the chernozem soil type.The effects of three N rates—0, 180 and 240 kg N ha−1—within three tillage practices—no-tillage,reduced and conventional tillage—and their influence on grain yield (GY) and the content of proteins,starch, oil, total tocopherols and carotenoids, phytic phosphorus (Phy), glutathione (GSH), phenolsand inorganic P (Pi) in maize grain were analysed in the period 2016–2018. Differences in maizeGY were 2.57 and 3.01 t ha−1for reduced- or no-tillage, respectively, in comparison to conventionaltillage. Conventional tillage and higher N rates contributed to the increase in maize yield and thegrain nutritional quality with regard to the increased level of proteins, GSH and Phy. On the otherhand reduced inputs were beneficial only for grain quality, in relation to the increase in tocopherolsand phenols.
PB  - Basel : MDPI
T2  - Agronomy
T1  - The contribution of soil tillage and nitrogen rate to the quality of maize grain
VL  - 10
IS  - 7
SP  - 976
DO  - 10.3390/agronomy10070976
ER  - 
@article{
author = "Simić, Milena and Dragičević, Vesna and Mladenović Drinić, Snežana and Vukadinović, Jelena and Kresović, Branka and Tabaković, Marijenka and Brankov, Milan",
year = "2020",
abstract = "A soil tillage system adjusted to the soil type and agro-ecological conditions, togetherwith the optimal nutrient supply, is a prerequisite for high maize (Zea mays L.) yield. However,there is little knowledge about the influence of soil tillage and nitrogen (N) rates on maize grainquality. A study was initiated in 1978 in Zemun Polje, Belgrade, Serbia, on the chernozem soil type.The effects of three N rates—0, 180 and 240 kg N ha−1—within three tillage practices—no-tillage,reduced and conventional tillage—and their influence on grain yield (GY) and the content of proteins,starch, oil, total tocopherols and carotenoids, phytic phosphorus (Phy), glutathione (GSH), phenolsand inorganic P (Pi) in maize grain were analysed in the period 2016–2018. Differences in maizeGY were 2.57 and 3.01 t ha−1for reduced- or no-tillage, respectively, in comparison to conventionaltillage. Conventional tillage and higher N rates contributed to the increase in maize yield and thegrain nutritional quality with regard to the increased level of proteins, GSH and Phy. On the otherhand reduced inputs were beneficial only for grain quality, in relation to the increase in tocopherolsand phenols.",
publisher = "Basel : MDPI",
journal = "Agronomy",
title = "The contribution of soil tillage and nitrogen rate to the quality of maize grain",
volume = "10",
number = "7",
pages = "976",
doi = "10.3390/agronomy10070976"
}
Simić, M., Dragičević, V., Mladenović Drinić, S., Vukadinović, J., Kresović, B., Tabaković, M.,& Brankov, M.. (2020). The contribution of soil tillage and nitrogen rate to the quality of maize grain. in Agronomy
Basel : MDPI., 10(7), 976.
https://doi.org/10.3390/agronomy10070976
Simić M, Dragičević V, Mladenović Drinić S, Vukadinović J, Kresović B, Tabaković M, Brankov M. The contribution of soil tillage and nitrogen rate to the quality of maize grain. in Agronomy. 2020;10(7):976.
doi:10.3390/agronomy10070976 .
Simić, Milena, Dragičević, Vesna, Mladenović Drinić, Snežana, Vukadinović, Jelena, Kresović, Branka, Tabaković, Marijenka, Brankov, Milan, "The contribution of soil tillage and nitrogen rate to the quality of maize grain" in Agronomy, 10, no. 7 (2020):976,
https://doi.org/10.3390/agronomy10070976 . .
5
5

Differences in nutritive and bioactive compounds content between hybrid and open-pollinated maize varieties

Babić, Vojka; Kravić, Natalija; Vančetović, Jelena; Delić, Nenad; Žilić, Slađana

(Novi Sad : University of Novi Sad - Scientific Institute of Food Technology, 2020)

TY  - JOUR
AU  - Babić, Vojka
AU  - Kravić, Natalija
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Žilić, Slađana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/774
AB  - The  contemporary  trends  in  maize  breeding  are  directed  at  identification  of  genotypes  with improved grain quality for human consumption, industrial processing, and their incorporation into breeding  programmes.  In  this  study,  three  maize  hybrids  and  three  open-pollinated  varieties  (OPVs),  differing in grain colour and type, were used to examine the differences in the total carotenoid content, the phenolic compounds profile and total antioxidant capacity (TAC). In addition, physical parameters, basic  chemical  composition,  as  well  as  the  content  of  protein  fractions  in  grain,  were  analysed.  The  multivariate  approach  through  Principal  Component  Analysis  (PCA)  application  contributed  to  better  understanding  of  the  complexity  of  the  interdependence  for  maize  grain  quality  parameters  tested.  From  the  aspect  of  different  end  use,  better  technological  quality  desirable  for  dry  milling  and  snack  food processing found in OPVs Osmak and Bosanac, as well as high content of bioactive compounds (i.e. TAC) exhibited by OPV Rumenka, make these genotypes superior compared to hybrids’ varieties evaluated.
AB  - Savremeni trendovi u oplemenjivanju kukuruza idu u pravcu identifikacije genotipova povećanog kvaliteta zrna za ljudsku upotrebu, industrijsku preradu i njihovog uključivanja u komercijalne oplemenjivačke programe. Za ovo istraživanje odabrane su tri hibridne i tri slobodnooprašujuće sorte kukuruza različite po boji i tipu zrna, u cilju ispitivanja razlika u sadržaju ukupnih karotenoida, fenolnih jedinjenja i ukupnom antioksidativnom kapacitetu. Dodatno su analizirane fizičke karakteristike i osnovni hemijski sastav zrna, kao i sadržaj proteinskih frakcija. Multivarijacioni pristup, kroz primenu analize glavnih komponenata (PCA) doprineo je boljem razumevanju složenih veza ispitivanih parametara kvaliteta. Sa aspekta različite upotrebne vrednosti, bolji tehnološki kvalitet poželjan za proces suvog mlevenja i proces proizvodnje grickalica (snek proizvoda) je identifikovan kod slobodnooprašujućih sorti Osmak i Bosanac, dok se sorta Rumenka odlikovala visokim sadržajem bioaktivnih jedinjenja (tj. visokim antioksidativnim kapacitetom). Dobijeni rezultati ukazuju na superiornost slobodnooprašujućih sorti u poređenju sa hibridnim.
PB  - Novi Sad : University of Novi Sad - Scientific Institute of Food Technology
T2  - Food & Feed Research
T1  - Differences in nutritive and bioactive compounds content between hybrid and open-pollinated maize varieties
VL  - 47
IS  - 1
SP  - 1
EP  - 12
DO  - 10.5937/FFR2001001B
ER  - 
@article{
author = "Babić, Vojka and Kravić, Natalija and Vančetović, Jelena and Delić, Nenad and Žilić, Slađana",
year = "2020",
abstract = "The  contemporary  trends  in  maize  breeding  are  directed  at  identification  of  genotypes  with improved grain quality for human consumption, industrial processing, and their incorporation into breeding  programmes.  In  this  study,  three  maize  hybrids  and  three  open-pollinated  varieties  (OPVs),  differing in grain colour and type, were used to examine the differences in the total carotenoid content, the phenolic compounds profile and total antioxidant capacity (TAC). In addition, physical parameters, basic  chemical  composition,  as  well  as  the  content  of  protein  fractions  in  grain,  were  analysed.  The  multivariate  approach  through  Principal  Component  Analysis  (PCA)  application  contributed  to  better  understanding  of  the  complexity  of  the  interdependence  for  maize  grain  quality  parameters  tested.  From  the  aspect  of  different  end  use,  better  technological  quality  desirable  for  dry  milling  and  snack  food processing found in OPVs Osmak and Bosanac, as well as high content of bioactive compounds (i.e. TAC) exhibited by OPV Rumenka, make these genotypes superior compared to hybrids’ varieties evaluated., Savremeni trendovi u oplemenjivanju kukuruza idu u pravcu identifikacije genotipova povećanog kvaliteta zrna za ljudsku upotrebu, industrijsku preradu i njihovog uključivanja u komercijalne oplemenjivačke programe. Za ovo istraživanje odabrane su tri hibridne i tri slobodnooprašujuće sorte kukuruza različite po boji i tipu zrna, u cilju ispitivanja razlika u sadržaju ukupnih karotenoida, fenolnih jedinjenja i ukupnom antioksidativnom kapacitetu. Dodatno su analizirane fizičke karakteristike i osnovni hemijski sastav zrna, kao i sadržaj proteinskih frakcija. Multivarijacioni pristup, kroz primenu analize glavnih komponenata (PCA) doprineo je boljem razumevanju složenih veza ispitivanih parametara kvaliteta. Sa aspekta različite upotrebne vrednosti, bolji tehnološki kvalitet poželjan za proces suvog mlevenja i proces proizvodnje grickalica (snek proizvoda) je identifikovan kod slobodnooprašujućih sorti Osmak i Bosanac, dok se sorta Rumenka odlikovala visokim sadržajem bioaktivnih jedinjenja (tj. visokim antioksidativnim kapacitetom). Dobijeni rezultati ukazuju na superiornost slobodnooprašujućih sorti u poređenju sa hibridnim.",
publisher = "Novi Sad : University of Novi Sad - Scientific Institute of Food Technology",
journal = "Food & Feed Research",
title = "Differences in nutritive and bioactive compounds content between hybrid and open-pollinated maize varieties",
volume = "47",
number = "1",
pages = "1-12",
doi = "10.5937/FFR2001001B"
}
Babić, V., Kravić, N., Vančetović, J., Delić, N.,& Žilić, S.. (2020). Differences in nutritive and bioactive compounds content between hybrid and open-pollinated maize varieties. in Food & Feed Research
Novi Sad : University of Novi Sad - Scientific Institute of Food Technology., 47(1), 1-12.
https://doi.org/10.5937/FFR2001001B
Babić V, Kravić N, Vančetović J, Delić N, Žilić S. Differences in nutritive and bioactive compounds content between hybrid and open-pollinated maize varieties. in Food & Feed Research. 2020;47(1):1-12.
doi:10.5937/FFR2001001B .
Babić, Vojka, Kravić, Natalija, Vančetović, Jelena, Delić, Nenad, Žilić, Slađana, "Differences in nutritive and bioactive compounds content between hybrid and open-pollinated maize varieties" in Food & Feed Research, 47, no. 1 (2020):1-12,
https://doi.org/10.5937/FFR2001001B . .

Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality

Ignjatović-Micić, Dragana; Kostadinović, Marija; Božinović, Sofija; Djordjevic Melnik, Olivera; Stanković, Goran; Delić, Nenad; Vančetović, Jelena

(Chile : Instituto de Investigaciones Agropecuarias, INIA, 2020)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Djordjevic Melnik, Olivera
AU  - Stanković, Goran
AU  - Delić, Nenad
AU  - Vančetović, Jelena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/819
AB  - Quality protein maize (QPM) (Zea mays L.) is primarily used for food in countries of tropical and sub-tropical regions
where maize is the main source of protein. Although its cultivation in temperate regions is hampered by residues of
exotic germplasm, it could be beneficial for use in livestock feeds as it was shown that substitution of standard maize
with QPM can improve livestock characteristics and decrease dietary lysine supplementation. The aim of this study
was to test 11 QPM hybrids obtained by crossing adapted QPM inbred lines for their performance in field trials in 2
yr at four locations, as well as to determine their relevant biochemical components. The main reason for rejecting nine
QPM hybrids was low grain yield, standard hybrids had higher yields on average for 37.8%. Hybrid ZPQPM6 had good
agronomic characteristics, but its biochemical components were nonsignificantly different from the standard hybrid.
Only hybrid ZPQPM13 met necessary criteria, grain yield comparable with standard hybrids, high tryptophan content
in different environments (average 0.083%) and hard endosperm (average score 1.87). Lysine content, measured after
mercantile production, was 0.44%. Quality index, although below the QPM threshold (which is 0.80%) was significantly
higher (p < 0.05) in ZPQPM13 in comparison with standard hybrid, indicating improved nutritional quality of the protein.
The results indicated that presence of exotic germplasm in these QPM hybrids is a consequential difficulty and that in their
parental inbred lines at least one more backcross with temperate germplasm should be done to select better adapted QPM.
PB  - Chile : Instituto de Investigaciones Agropecuarias, INIA
T2  - Chilean Journal of Agricultural Research
T1  - Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality
VL  - 80
IS  - 4
SP  - 598
EP  - 607
DO  - 10.4067/S0718-58392020000400598
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Božinović, Sofija and Djordjevic Melnik, Olivera and Stanković, Goran and Delić, Nenad and Vančetović, Jelena",
year = "2020",
abstract = "Quality protein maize (QPM) (Zea mays L.) is primarily used for food in countries of tropical and sub-tropical regions
where maize is the main source of protein. Although its cultivation in temperate regions is hampered by residues of
exotic germplasm, it could be beneficial for use in livestock feeds as it was shown that substitution of standard maize
with QPM can improve livestock characteristics and decrease dietary lysine supplementation. The aim of this study
was to test 11 QPM hybrids obtained by crossing adapted QPM inbred lines for their performance in field trials in 2
yr at four locations, as well as to determine their relevant biochemical components. The main reason for rejecting nine
QPM hybrids was low grain yield, standard hybrids had higher yields on average for 37.8%. Hybrid ZPQPM6 had good
agronomic characteristics, but its biochemical components were nonsignificantly different from the standard hybrid.
Only hybrid ZPQPM13 met necessary criteria, grain yield comparable with standard hybrids, high tryptophan content
in different environments (average 0.083%) and hard endosperm (average score 1.87). Lysine content, measured after
mercantile production, was 0.44%. Quality index, although below the QPM threshold (which is 0.80%) was significantly
higher (p < 0.05) in ZPQPM13 in comparison with standard hybrid, indicating improved nutritional quality of the protein.
The results indicated that presence of exotic germplasm in these QPM hybrids is a consequential difficulty and that in their
parental inbred lines at least one more backcross with temperate germplasm should be done to select better adapted QPM.",
publisher = "Chile : Instituto de Investigaciones Agropecuarias, INIA",
journal = "Chilean Journal of Agricultural Research",
title = "Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality",
volume = "80",
number = "4",
pages = "598-607",
doi = "10.4067/S0718-58392020000400598"
}
Ignjatović-Micić, D., Kostadinović, M., Božinović, S., Djordjevic Melnik, O., Stanković, G., Delić, N.,& Vančetović, J.. (2020). Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality. in Chilean Journal of Agricultural Research
Chile : Instituto de Investigaciones Agropecuarias, INIA., 80(4), 598-607.
https://doi.org/10.4067/S0718-58392020000400598
Ignjatović-Micić D, Kostadinović M, Božinović S, Djordjevic Melnik O, Stanković G, Delić N, Vančetović J. Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality. in Chilean Journal of Agricultural Research. 2020;80(4):598-607.
doi:10.4067/S0718-58392020000400598 .
Ignjatović-Micić, Dragana, Kostadinović, Marija, Božinović, Sofija, Djordjevic Melnik, Olivera, Stanković, Goran, Delić, Nenad, Vančetović, Jelena, "Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality" in Chilean Journal of Agricultural Research, 80, no. 4 (2020):598-607,
https://doi.org/10.4067/S0718-58392020000400598 . .
1
1

Genotype and sowing time effects on soybean yield and quality

Mandić, Violeta; Đorđević, Snežana; Đorđević, Nikola; Bijelić, Zorica; Krnjaja, Vesna; Petričević, Maja; Brankov, Milan

(Basel : MDPI AG, 2020)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Đorđević, Snežana
AU  - Đorđević, Nikola
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Petričević, Maja
AU  - Brankov, Milan
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/818
AB  - The successful production of soybeans is largely dependent on the sowing time, because
every sowing outside the optimal time contributes significantly to yield losses. This field study
aimed to evaluate the effects of sowing time (optimal—April 5; late—April 27) on the quantitative
and quality traits of three soybean genotypes (Galina—0 maturity group; Sava—I maturity group;
and Rubin—II maturity group) under dryland conditions in Vojvodina Province (Serbia) during
2017 and 2018. The genotype Sava had higher yield in climatic-unfavorable 2017, while Rubin had a
higher yield in climatic-favorable 2018. The yields significantly decreased when the soybeans were
sown in late April due to reductions in the number of pods per plant, seed weight per plant, and
1000-seed weight. The reduction in yield components was likely due to the accelerated senescence of
plants and the negative effect of high temperature and low precipitation during the seed filling stage.
Accordingly, the various sowing times and properly chosen genotypes provide a better utilization of
soil and water resources. A proper genotype selection and sowing time can contribute to a high yield.
At the same time, the protein and oil contents can be altered by the sowing time, especially under
water stress during the reproductive stage.
PB  - Basel : MDPI AG
T2  - Agriculture (Switzerland)
T1  - Genotype and sowing time effects on soybean yield and quality
VL  - 10
IS  - 11
SP  - 502
SP  - 1
EP  - 9
DO  - 10.3390/agriculture10110502
ER  - 
@article{
author = "Mandić, Violeta and Đorđević, Snežana and Đorđević, Nikola and Bijelić, Zorica and Krnjaja, Vesna and Petričević, Maja and Brankov, Milan",
year = "2020",
abstract = "The successful production of soybeans is largely dependent on the sowing time, because
every sowing outside the optimal time contributes significantly to yield losses. This field study
aimed to evaluate the effects of sowing time (optimal—April 5; late—April 27) on the quantitative
and quality traits of three soybean genotypes (Galina—0 maturity group; Sava—I maturity group;
and Rubin—II maturity group) under dryland conditions in Vojvodina Province (Serbia) during
2017 and 2018. The genotype Sava had higher yield in climatic-unfavorable 2017, while Rubin had a
higher yield in climatic-favorable 2018. The yields significantly decreased when the soybeans were
sown in late April due to reductions in the number of pods per plant, seed weight per plant, and
1000-seed weight. The reduction in yield components was likely due to the accelerated senescence of
plants and the negative effect of high temperature and low precipitation during the seed filling stage.
Accordingly, the various sowing times and properly chosen genotypes provide a better utilization of
soil and water resources. A proper genotype selection and sowing time can contribute to a high yield.
At the same time, the protein and oil contents can be altered by the sowing time, especially under
water stress during the reproductive stage.",
publisher = "Basel : MDPI AG",
journal = "Agriculture (Switzerland)",
title = "Genotype and sowing time effects on soybean yield and quality",
volume = "10",
number = "11",
pages = "502-1-9",
doi = "10.3390/agriculture10110502"
}
Mandić, V., Đorđević, S., Đorđević, N., Bijelić, Z., Krnjaja, V., Petričević, M.,& Brankov, M.. (2020). Genotype and sowing time effects on soybean yield and quality. in Agriculture (Switzerland)
Basel : MDPI AG., 10(11), 502-9.
https://doi.org/10.3390/agriculture10110502
Mandić V, Đorđević S, Đorđević N, Bijelić Z, Krnjaja V, Petričević M, Brankov M. Genotype and sowing time effects on soybean yield and quality. in Agriculture (Switzerland). 2020;10(11):502-9.
doi:10.3390/agriculture10110502 .
Mandić, Violeta, Đorđević, Snežana, Đorđević, Nikola, Bijelić, Zorica, Krnjaja, Vesna, Petričević, Maja, Brankov, Milan, "Genotype and sowing time effects on soybean yield and quality" in Agriculture (Switzerland), 10, no. 11 (2020):502-9,
https://doi.org/10.3390/agriculture10110502 . .
2
2

Use of plant genetic resources in crop improvement–example of Serbia

Anđelković, Violeta; Cvejić, Sandra; Jocić, Siniša; Kondić-Špika, Ankica; Marjanović Jeromela, Ana; Mikić, Sanja; Prodanović, Slaven; Radanović, Aleksandra; Savić-Ivanov, Milena; Trkulja, Dragana; Miladinović, Dragana

(Springer Science+Business Media B.V., 2020)

TY  - JOUR
AU  - Anđelković, Violeta
AU  - Cvejić, Sandra
AU  - Jocić, Siniša
AU  - Kondić-Špika, Ankica
AU  - Marjanović Jeromela, Ana
AU  - Mikić, Sanja
AU  - Prodanović, Slaven
AU  - Radanović, Aleksandra
AU  - Savić-Ivanov, Milena
AU  - Trkulja, Dragana
AU  - Miladinović, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/815
AB  - Plant genetic resources are a link between agriculture, environment and trade, so their conservation requires cooperation from different sectors. The existing diversity in genetic resources is the foundation in breeding for new challenges or new markets in the future. The number of crop genetic resources in Serbia is obscure because there is no national inventory. It is thought that there are about 15,000 seed accessions and 3500 accessions of fruit trees and grape in collections of different national institutes and stakeholders. In the National Plant Gene Bank, there are more than 4000 accessions of nearly 250 plant species. Crops kept in ex situ collections are used in breeding programs and interspecific crosses, for selection and introduction of desired traits through pre-breeding programs. Serbia as a state participates in all national and international efforts for preservation, management and use of plant genetic resources. Additionally, Serbia has also established a set of national regulations related to plant genetic resources and their use in breeding. Since Serbia is one of Europe’s most important crop producers, especially in maize (11% of EU-27 production), soya (35%), sunflowers (6%) and sugar beet (2.5%), this paper discusses certain issues and achievements in the use of plant genetic resources in cereal and oil crops improvement in Serbia, as well as national and international regulations affecting their exploitation.
PB  - Springer Science+Business Media B.V.
T2  - Genetic Resources and Crop Evolution
T1  - Use of plant genetic resources in crop improvement–example of Serbia
DO  - 10.1007/s10722-020-01029-9
ER  - 
@article{
author = "Anđelković, Violeta and Cvejić, Sandra and Jocić, Siniša and Kondić-Špika, Ankica and Marjanović Jeromela, Ana and Mikić, Sanja and Prodanović, Slaven and Radanović, Aleksandra and Savić-Ivanov, Milena and Trkulja, Dragana and Miladinović, Dragana",
year = "2020",
abstract = "Plant genetic resources are a link between agriculture, environment and trade, so their conservation requires cooperation from different sectors. The existing diversity in genetic resources is the foundation in breeding for new challenges or new markets in the future. The number of crop genetic resources in Serbia is obscure because there is no national inventory. It is thought that there are about 15,000 seed accessions and 3500 accessions of fruit trees and grape in collections of different national institutes and stakeholders. In the National Plant Gene Bank, there are more than 4000 accessions of nearly 250 plant species. Crops kept in ex situ collections are used in breeding programs and interspecific crosses, for selection and introduction of desired traits through pre-breeding programs. Serbia as a state participates in all national and international efforts for preservation, management and use of plant genetic resources. Additionally, Serbia has also established a set of national regulations related to plant genetic resources and their use in breeding. Since Serbia is one of Europe’s most important crop producers, especially in maize (11% of EU-27 production), soya (35%), sunflowers (6%) and sugar beet (2.5%), this paper discusses certain issues and achievements in the use of plant genetic resources in cereal and oil crops improvement in Serbia, as well as national and international regulations affecting their exploitation.",
publisher = "Springer Science+Business Media B.V.",
journal = "Genetic Resources and Crop Evolution",
title = "Use of plant genetic resources in crop improvement–example of Serbia",
doi = "10.1007/s10722-020-01029-9"
}
Anđelković, V., Cvejić, S., Jocić, S., Kondić-Špika, A., Marjanović Jeromela, A., Mikić, S., Prodanović, S., Radanović, A., Savić-Ivanov, M., Trkulja, D.,& Miladinović, D.. (2020). Use of plant genetic resources in crop improvement–example of Serbia. in Genetic Resources and Crop Evolution
Springer Science+Business Media B.V...
https://doi.org/10.1007/s10722-020-01029-9
Anđelković V, Cvejić S, Jocić S, Kondić-Špika A, Marjanović Jeromela A, Mikić S, Prodanović S, Radanović A, Savić-Ivanov M, Trkulja D, Miladinović D. Use of plant genetic resources in crop improvement–example of Serbia. in Genetic Resources and Crop Evolution. 2020;.
doi:10.1007/s10722-020-01029-9 .
Anđelković, Violeta, Cvejić, Sandra, Jocić, Siniša, Kondić-Špika, Ankica, Marjanović Jeromela, Ana, Mikić, Sanja, Prodanović, Slaven, Radanović, Aleksandra, Savić-Ivanov, Milena, Trkulja, Dragana, Miladinović, Dragana, "Use of plant genetic resources in crop improvement–example of Serbia" in Genetic Resources and Crop Evolution (2020),
https://doi.org/10.1007/s10722-020-01029-9 . .

Morphological and physiological response of maize seedlings to chilling stress

Nikolić, Ana; Kravić, Natalija; Ristić, Danijela; Anđelković, Violeta; Marković, Ksenija; Vančetović, Jelena; Ignjatović-Micić, Dragana

(Beograd : Društavo genetičara Srbije, 2020)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kravić, Natalija
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/814
AB  - Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing.
AB  - Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Morphological and physiological response of maize seedlings to chilling stress
VL  - 52
IS  - 2
SP  - 689
EP  - 698
DO  - 10.2298/GENSR2002689N
ER  - 
@article{
author = "Nikolić, Ana and Kravić, Natalija and Ristić, Danijela and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing., Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Morphological and physiological response of maize seedlings to chilling stress",
volume = "52",
number = "2",
pages = "689-698",
doi = "10.2298/GENSR2002689N"
}
Nikolić, A., Kravić, N., Ristić, D., Anđelković, V., Marković, K., Vančetović, J.,& Ignjatović-Micić, D.. (2020). Morphological and physiological response of maize seedlings to chilling stress. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 689-698.
https://doi.org/10.2298/GENSR2002689N
Nikolić A, Kravić N, Ristić D, Anđelković V, Marković K, Vančetović J, Ignjatović-Micić D. Morphological and physiological response of maize seedlings to chilling stress. in Genetika. 2020;52(2):689-698.
doi:10.2298/GENSR2002689N .
Nikolić, Ana, Kravić, Natalija, Ristić, Danijela, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, Ignjatović-Micić, Dragana, "Morphological and physiological response of maize seedlings to chilling stress" in Genetika, 52, no. 2 (2020):689-698,
https://doi.org/10.2298/GENSR2002689N . .
1
1

Variability of maize inbred lines in nitrogen use effciency

Dragičević, Vesna; Mladenović Drinić, Snežana; Simić, Milena; Brankov, Milan; Dumanović, Zoran; Sečanski, Mile; Milenković, Milena

(Beograd : Društavo genetičara Srbije, 2020)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Mladenović Drinić, Snežana
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Dumanović, Zoran
AU  - Sečanski, Mile
AU  - Milenković, Milena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/813
AB  - Nitrogen (N) is an important element for many physiological processes in crops, and grain yield realisation. Nitrogen loss could be significant through leaching and evaporation, and from this reason lower quantities for fertilization are required. A genotype could be an important source for improved N management in crops. Breeding for high yield and nutrient-efficient genotypes is the most important strategy to enable food security, resolve resource scarcity and environmental pollution. Variability of 36 maize lines grown in optimal and low-N (without fertilization) conditions was assessed through grain yield, 1000 kernel weight, N utilization efficiency (NUtE) and N apparent recovery fraction (nitrogen use efficiency – NUE), during seasons 2017 and 2018. The
genotype and year are important sources for variation of grain yield, 1000 kernel weight and NUtE, as a factor which defines N utilization efficiency. The lines, such as L1, L6, L13, L16, L26, L27, L32 and L34 are able to achieve higher grain yield when grown on low-N. Furthermore, L16, L22, L24 and L26 have high NUtE values in both experimental years (even in 2017, season with low and unequal precipitation level), especially in low-N treatment. From that point of view, they could be characterized as efficient N users, even in low-N conditions, as well as tolerant to stressful conditions. Nevertheless, L1, L6 and L27 are the lines with negative NUE, what gives them attribute as the best N users in low-N conditions. Based on the similarity of NUtE values, the genotypes such as L2, L3, L4, L8, L11, L12, L14, L15, L16, L18, L19, L24, L26, L32, L33, L34 could be considered as the primary focus for further breeding programs, due to the fact that they don’t have only improved NUE, but also high grain yield (even in unfavourable years), which indicates improved tolerance to various abiotic stressful factors.
AB  - Azot je element koji je važan za brojne fiziološke procese, kao i ostvarenje prinosa useva. Veliki
gubici azota se ostvaruju putem ispiranja i evaporacije i stoga se preporučuju niže doze ovog
elementa za đubrenje. Genotip može predstavljati važnu bazu za efikasniji menadžment azotom
kod useva. Selekcija genotipova sa visokim prinosom i efikasnošću iskorišćenja nutritiva
predstavlja važnu strategiju za istovremeno obezbeđivanje sigurnosti hrane, rešavanje nedostatka
resursa i zagađenja životne sredine. Varijabilnost u reakciji 36 linija kukuruza gajenih u
uslovima optimalne N obezbeđenosti i niskog N (bez đubrenja) praćena je tokom 2017. i 2018.
godine, preko prinosa zrna, mase 1000 zrna, efikasnosti iskorišćenja N (NUtE) i nadoknade N
(NUE). Genotip i godina su predstavljali važne izvore variranja prinosa zrna, mase 1000 zrna i
NutE, kao faktora koji definišu efikasnost iskorišćenja N. Linije L1, L6, L13, L16, L26, L27,
L32 i L34 su imale veće vrednosti prinosa u uslovima niskog N. Osim toga, L16, L22, L24 i L26
su imale veće vrednosti NutE tokom obe eksperimentalne sezone (čak i u 2017, sezoni sa nižim
nivoom i lošijim rasporedom padavina), posebno pri niskom N. Sa te tačke gledašta, navedene
linije bi mogle biti okarakerisane kao efikasni N potrošači, kao i genotipovi sa većom
toleratnošću na stresne uslove. Takođe, L1, L6 i L27, sa negativnim NUE vrednostima bi mogle
predstavljati najekonomičnije N potrošače u uslovima niske N obezbeđenosti. Na osnovu
sličnosti NutE vrednosti, genotipovi L2, L3, L4, L8, L11, L12, L14, L15, L16, L18, L19, L24,
L26, L32, L33, L34 bi mogli da predstavljaju fokus, odnosno, mogli bi načelno da se uzmu u
razmatranje u selekcionim programima, s obzirom da nemaju samo poboljšan NUE, već i visok
prinos (čak i tokom nepovoljne sezone), u odnosu na ostale genotipove, što bi ih moglo
okarakterisati kao genotipove sa poboljšanom tolerantnošću na abiotički stres.
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Variability of maize inbred lines in nitrogen use effciency
VL  - 52
IS  - 2
SP  - 585
EP  - 596
DO  - 10.2298/GENSR2002585D
ER  - 
@article{
author = "Dragičević, Vesna and Mladenović Drinić, Snežana and Simić, Milena and Brankov, Milan and Dumanović, Zoran and Sečanski, Mile and Milenković, Milena",
year = "2020",
abstract = "Nitrogen (N) is an important element for many physiological processes in crops, and grain yield realisation. Nitrogen loss could be significant through leaching and evaporation, and from this reason lower quantities for fertilization are required. A genotype could be an important source for improved N management in crops. Breeding for high yield and nutrient-efficient genotypes is the most important strategy to enable food security, resolve resource scarcity and environmental pollution. Variability of 36 maize lines grown in optimal and low-N (without fertilization) conditions was assessed through grain yield, 1000 kernel weight, N utilization efficiency (NUtE) and N apparent recovery fraction (nitrogen use efficiency – NUE), during seasons 2017 and 2018. The
genotype and year are important sources for variation of grain yield, 1000 kernel weight and NUtE, as a factor which defines N utilization efficiency. The lines, such as L1, L6, L13, L16, L26, L27, L32 and L34 are able to achieve higher grain yield when grown on low-N. Furthermore, L16, L22, L24 and L26 have high NUtE values in both experimental years (even in 2017, season with low and unequal precipitation level), especially in low-N treatment. From that point of view, they could be characterized as efficient N users, even in low-N conditions, as well as tolerant to stressful conditions. Nevertheless, L1, L6 and L27 are the lines with negative NUE, what gives them attribute as the best N users in low-N conditions. Based on the similarity of NUtE values, the genotypes such as L2, L3, L4, L8, L11, L12, L14, L15, L16, L18, L19, L24, L26, L32, L33, L34 could be considered as the primary focus for further breeding programs, due to the fact that they don’t have only improved NUE, but also high grain yield (even in unfavourable years), which indicates improved tolerance to various abiotic stressful factors., Azot je element koji je važan za brojne fiziološke procese, kao i ostvarenje prinosa useva. Veliki
gubici azota se ostvaruju putem ispiranja i evaporacije i stoga se preporučuju niže doze ovog
elementa za đubrenje. Genotip može predstavljati važnu bazu za efikasniji menadžment azotom
kod useva. Selekcija genotipova sa visokim prinosom i efikasnošću iskorišćenja nutritiva
predstavlja važnu strategiju za istovremeno obezbeđivanje sigurnosti hrane, rešavanje nedostatka
resursa i zagađenja životne sredine. Varijabilnost u reakciji 36 linija kukuruza gajenih u
uslovima optimalne N obezbeđenosti i niskog N (bez đubrenja) praćena je tokom 2017. i 2018.
godine, preko prinosa zrna, mase 1000 zrna, efikasnosti iskorišćenja N (NUtE) i nadoknade N
(NUE). Genotip i godina su predstavljali važne izvore variranja prinosa zrna, mase 1000 zrna i
NutE, kao faktora koji definišu efikasnost iskorišćenja N. Linije L1, L6, L13, L16, L26, L27,
L32 i L34 su imale veće vrednosti prinosa u uslovima niskog N. Osim toga, L16, L22, L24 i L26
su imale veće vrednosti NutE tokom obe eksperimentalne sezone (čak i u 2017, sezoni sa nižim
nivoom i lošijim rasporedom padavina), posebno pri niskom N. Sa te tačke gledašta, navedene
linije bi mogle biti okarakerisane kao efikasni N potrošači, kao i genotipovi sa većom
toleratnošću na stresne uslove. Takođe, L1, L6 i L27, sa negativnim NUE vrednostima bi mogle
predstavljati najekonomičnije N potrošače u uslovima niske N obezbeđenosti. Na osnovu
sličnosti NutE vrednosti, genotipovi L2, L3, L4, L8, L11, L12, L14, L15, L16, L18, L19, L24,
L26, L32, L33, L34 bi mogli da predstavljaju fokus, odnosno, mogli bi načelno da se uzmu u
razmatranje u selekcionim programima, s obzirom da nemaju samo poboljšan NUE, već i visok
prinos (čak i tokom nepovoljne sezone), u odnosu na ostale genotipove, što bi ih moglo
okarakterisati kao genotipove sa poboljšanom tolerantnošću na abiotički stres.",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Variability of maize inbred lines in nitrogen use effciency",
volume = "52",
number = "2",
pages = "585-596",
doi = "10.2298/GENSR2002585D"
}
Dragičević, V., Mladenović Drinić, S., Simić, M., Brankov, M., Dumanović, Z., Sečanski, M.,& Milenković, M.. (2020). Variability of maize inbred lines in nitrogen use effciency. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 585-596.
https://doi.org/10.2298/GENSR2002585D
Dragičević V, Mladenović Drinić S, Simić M, Brankov M, Dumanović Z, Sečanski M, Milenković M. Variability of maize inbred lines in nitrogen use effciency. in Genetika. 2020;52(2):585-596.
doi:10.2298/GENSR2002585D .
Dragičević, Vesna, Mladenović Drinić, Snežana, Simić, Milena, Brankov, Milan, Dumanović, Zoran, Sečanski, Mile, Milenković, Milena, "Variability of maize inbred lines in nitrogen use effciency" in Genetika, 52, no. 2 (2020):585-596,
https://doi.org/10.2298/GENSR2002585D . .

Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes

Tabaković, Marijenka; Simić, Milena; Stanisavljević, Rade; Milivojević, Marija; Sečanski, Mile; Poštić, Dobrivoj

(Santiago : Instituto de Investigaciones Agropecuarias, INIA, 2020)

TY  - JOUR
AU  - Tabaković, Marijenka
AU  - Simić, Milena
AU  - Stanisavljević, Rade
AU  - Milivojević, Marija
AU  - Sečanski, Mile
AU  - Poštić, Dobrivoj
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/808
AB  - The uniform seed material is a prerequisite of stable yields. Therefore, the aim of the study was to observe variability of physiological seed traits depending on the classification of seeds by size and shape, and to determine advantages of large over small seed fractions. Three maize (Zea mays L.) hybrids (ZP 505, ZP 677, ZP 684), produced in two locations (Orahovo, Plavna), were  classified into six fractions; small flat seed (SFS), medium small flat seed (MSFS), large flat
seed (LFS), small round seed (SRS), medium small round seed (MSRS) and large round seed (LRS). Two laboratory treatments were applied on seed: TR1 (cold test) and TR2 (20/30 ºC). In both temperature treatments, the first evaluation was done after 72-h germination, and then every 24 h until 7th day. The highest total germination (G) was recorded for ZP 677 (93.7%), location Orahovo (94.9%), temperature TR2 (95.2%) and MSFS fraction (89.7%). The total proportion of
factors in the variance for G was R2  = 0.694. Locations and genotypes predominantly affected the germination rate (GR), η = 0.338. Flat fraction seeds had more rapid emergence (> 90%) of seedlings than the round fraction seeds (> 85%). Round seeds were more susceptible and seed size had a greater effect on vigour than LRS, 70%. The partial effect of the fraction on G was not estimated (η = 0.037), but its cumulative effect with other factors was evident (Hybrid × Fraction,
η = 0.070).
PB  - Santiago : Instituto de Investigaciones Agropecuarias, INIA
T2  - Chilean Journal of Agricultural Research
T1  - Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes
VL  - 80
IS  - 3
SP  - 381
EP  - 392
DO  - 10.4067/S0718-58392020000300381
ER  - 
@article{
author = "Tabaković, Marijenka and Simić, Milena and Stanisavljević, Rade and Milivojević, Marija and Sečanski, Mile and Poštić, Dobrivoj",
year = "2020",
abstract = "The uniform seed material is a prerequisite of stable yields. Therefore, the aim of the study was to observe variability of physiological seed traits depending on the classification of seeds by size and shape, and to determine advantages of large over small seed fractions. Three maize (Zea mays L.) hybrids (ZP 505, ZP 677, ZP 684), produced in two locations (Orahovo, Plavna), were  classified into six fractions; small flat seed (SFS), medium small flat seed (MSFS), large flat
seed (LFS), small round seed (SRS), medium small round seed (MSRS) and large round seed (LRS). Two laboratory treatments were applied on seed: TR1 (cold test) and TR2 (20/30 ºC). In both temperature treatments, the first evaluation was done after 72-h germination, and then every 24 h until 7th day. The highest total germination (G) was recorded for ZP 677 (93.7%), location Orahovo (94.9%), temperature TR2 (95.2%) and MSFS fraction (89.7%). The total proportion of
factors in the variance for G was R2  = 0.694. Locations and genotypes predominantly affected the germination rate (GR), η = 0.338. Flat fraction seeds had more rapid emergence (> 90%) of seedlings than the round fraction seeds (> 85%). Round seeds were more susceptible and seed size had a greater effect on vigour than LRS, 70%. The partial effect of the fraction on G was not estimated (η = 0.037), but its cumulative effect with other factors was evident (Hybrid × Fraction,
η = 0.070).",
publisher = "Santiago : Instituto de Investigaciones Agropecuarias, INIA",
journal = "Chilean Journal of Agricultural Research",
title = "Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes",
volume = "80",
number = "3",
pages = "381-392",
doi = "10.4067/S0718-58392020000300381"
}
Tabaković, M., Simić, M., Stanisavljević, R., Milivojević, M., Sečanski, M.,& Poštić, D.. (2020). Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes. in Chilean Journal of Agricultural Research
Santiago : Instituto de Investigaciones Agropecuarias, INIA., 80(3), 381-392.
https://doi.org/10.4067/S0718-58392020000300381
Tabaković M, Simić M, Stanisavljević R, Milivojević M, Sečanski M, Poštić D. Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes. in Chilean Journal of Agricultural Research. 2020;80(3):381-392.
doi:10.4067/S0718-58392020000300381 .
Tabaković, Marijenka, Simić, Milena, Stanisavljević, Rade, Milivojević, Marija, Sečanski, Mile, Poštić, Dobrivoj, "Effects of shape and size of hybrid maize seed on germination and vigour of different genotypes" in Chilean Journal of Agricultural Research, 80, no. 3 (2020):381-392,
https://doi.org/10.4067/S0718-58392020000300381 . .
3
4

The conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties

Tolimir, Miodrag; Kresović, Branka; Životić, Ljubomir; Dragović, Snežana; Dragović, Ranko; Sredojević, Zorica; Gajić, Boško

(London : Natue Research, 2020)

TY  - JOUR
AU  - Tolimir, Miodrag
AU  - Kresović, Branka
AU  - Životić, Ljubomir
AU  - Dragović, Snežana
AU  - Dragović, Ranko
AU  - Sredojević, Zorica
AU  - Gajić, Boško
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/807
AB  - This study was conducted to compare soil particle density (ρs), soil total porosity (TP), liquid limit (LL), plastic limit (PL), and plasticity index, and their relations with soil organic matter (SOM), of non-carbonate silty clay Fluvisols under different land uses. Three neighboring land uses were studied: native deciduous forest, arable land, and meadow, managed in the same way for more than 100 years. Soil was collected from 27 soil profiles and from three depths (0–15, 15–30 and 30–45 cm). Land use caused statistically significant but different impacts on soil properties, particularly in the topsoil. The forest topsoil measured the lowest ρs and bulk density (ρb) but the highest SOM and soil water content at PL, compared to meadow and arable soil. Statistically significant linear relationship was observed with the SOM content and ρs (− 0.851**), ρb (− 0.567**), calculated TP (0.567**) and measured TP (− 0.280**). There was a nonlinear relationship between SOM and LL (0.704**) and PL (0.845**) at the topsoil. The findings suggested that SOM content strongly affected ρs, ρb, TP, LL and LP. This regional study showed that the conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties.
PB  - London : Natue Research
T2  - Scientific Reports
T1  - The conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties
VL  - 10
IS  - 1
SP  - 13668
DO  - 10.1038/s41598-020-70464-6
ER  - 
@article{
author = "Tolimir, Miodrag and Kresović, Branka and Životić, Ljubomir and Dragović, Snežana and Dragović, Ranko and Sredojević, Zorica and Gajić, Boško",
year = "2020",
abstract = "This study was conducted to compare soil particle density (ρs), soil total porosity (TP), liquid limit (LL), plastic limit (PL), and plasticity index, and their relations with soil organic matter (SOM), of non-carbonate silty clay Fluvisols under different land uses. Three neighboring land uses were studied: native deciduous forest, arable land, and meadow, managed in the same way for more than 100 years. Soil was collected from 27 soil profiles and from three depths (0–15, 15–30 and 30–45 cm). Land use caused statistically significant but different impacts on soil properties, particularly in the topsoil. The forest topsoil measured the lowest ρs and bulk density (ρb) but the highest SOM and soil water content at PL, compared to meadow and arable soil. Statistically significant linear relationship was observed with the SOM content and ρs (− 0.851**), ρb (− 0.567**), calculated TP (0.567**) and measured TP (− 0.280**). There was a nonlinear relationship between SOM and LL (0.704**) and PL (0.845**) at the topsoil. The findings suggested that SOM content strongly affected ρs, ρb, TP, LL and LP. This regional study showed that the conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties.",
publisher = "London : Natue Research",
journal = "Scientific Reports",
title = "The conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties",
volume = "10",
number = "1",
pages = "13668",
doi = "10.1038/s41598-020-70464-6"
}
Tolimir, M., Kresović, B., Životić, L., Dragović, S., Dragović, R., Sredojević, Z.,& Gajić, B.. (2020). The conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties. in Scientific Reports
London : Natue Research., 10(1), 13668.
https://doi.org/10.1038/s41598-020-70464-6
Tolimir M, Kresović B, Životić L, Dragović S, Dragović R, Sredojević Z, Gajić B. The conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties. in Scientific Reports. 2020;10(1):13668.
doi:10.1038/s41598-020-70464-6 .
Tolimir, Miodrag, Kresović, Branka, Životić, Ljubomir, Dragović, Snežana, Dragović, Ranko, Sredojević, Zorica, Gajić, Boško, "The conversion of forestland into agricultural land without appropriate measures to conserve SOM leads to the degradation of physical and rheological soil properties" in Scientific Reports, 10, no. 1 (2020):13668,
https://doi.org/10.1038/s41598-020-70464-6 . .
1
2
1

Integrated weed management in long-term maize cultivation

Simić, Milena; Dragičević, Vesna; Chachalis, Demosthenis; Dolijanović, Željko; Brankov, Milan

(Vilnius : Lithuanian Research Centre for Agriculture and Forestry, 2020)

TY  - JOUR
AU  - Simić, Milena
AU  - Dragičević, Vesna
AU  - Chachalis, Demosthenis
AU  - Dolijanović, Željko
AU  - Brankov, Milan
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/804
AB  - The effects of different measures within maize cropping technology, aimed to suppress weeds as a part of integrated weed management (IWM) system, are analysed and evaluated in this manuscript, in line with the results of longterm experiments. For sustainable maize (Zea mays L.) production, implementation of IWM system aiming to reduce reliance on chemical weed control within Europe is a key priority. This IWM system includes all possible solutions, such as preventive, direct, biological, mechanical and alternative measures. A cropping system approach
is essential to manage weeds, utilize genetic potential of maize genotypes and reduce yield losses due to weed competition. Long-term experiments are nowadays rare, but they are an excellent and reliable method for comparing cropping systems regarding yield and reduction of weed infestation level. In the research program implemented at the Maize Research Institute Zemun Polje in Central Serbia, the effects of different cropping measures and their interactions
as a part of IWMs were studied during ten years. Maize rotations with winter wheat (Triticum aestivum L.) and soybean (Glycine max (L.) Merr.), combined with herbicide application, showed the best effect on weed biomass reduction, 92.1% and 92.2%, respectively. Winter wheat was a better preceding crop for maize than soybean, especially in combination with herbicides applied in recommended as well as in half of recommended rate. Intensification of soil tillage significantly reduced maize weed infestation, especially abundance of perennial species: Johnson grass (Sorghum halepense (L.) Pers.), Canada thistle (Cirsium arvense (L.) Scop.) and field bindweed (Convolvulus arvensis L.). Other measures, such as type of fertilizer, maize row space and crop density, cover cropping and intercropping also affected weed biomass production in maize fields. Maize growing with reduced row spacing contributed to weed biomass reduction by 27.4%, while application of slow-release urea contributed to crop competitiveness. Weed biomass in sweet maize (Zea mays L. convar. saccharata) grown with common vetch as a cover crop was significantly reduced (48.5 g m-2) compared with the treatment without a cover crop
 (564.3 g m-2).
AB  - Straipsnyje analizuojama kukurūzų įvairių auginimo priemonių ir auginimo technologijų įtaka piktžolių mažėjimui ilgalaikio eksperimento metu. Europoje tvaraus kukurūzų auginimo svarbiausias prioritetas yra integruota piktžolių kontrolės (IPK) sistema, kuria siekiama sumažinti priklausomumą nuo cheminės piktžolių kontrolės. IPK sistema apima visus įmanomus sprendimus, pavyzdžiui, prevencines tiesiogines, biologines, mechanines ir alternatyvias priemones. Augalininkystės sistemų metodas yra labai svarbus siekiant kontroliuoti piktžoles, išnaudoti kukurūzų genotipų genetinį potencialą ir sumažinti derliaus nuostolius dėl piktžolių.
Šiais laikais ilgalaikiai eksperimentai vykdomi retai, tačiau jie yra puikus ir patikimas būdas augalininkystės sistemas palyginti pagal derlių ir pasėlių piktžolėtumo mažinimą. Centrinėje Serbijoje esančiame Kukurūzų tyrimų institute Zemun Polje įgyvendinant mokslinių tyrimų programą, kelerius metus kaip IPK dalis buvo tirta įvairių agrotechnikos priemonių įtaka ir jų sąveika. Siekiant sumažinti piktžolių biomasę efektyviausia buvo paprastųjų kukurūzų (Zea mays L.) sėjomaina su žieminiais kviečiais ir sojomis kartu su herbicidų panaudojimu – atitinkamai
92,1 ir 92,2 %. Žieminiai kviečiai buvo geresnis kukurūzų priešsėlis nei sojos, ypač kartu su herbicidais, panaudojus ir rekomenduojamą normą, ir pusę rekomenduojamos jų normos. Žemės dirbimas turėjo reikšmingos įtakos kukurūzų pasėlio piktžolėtumui, ypač daugiametėms piktžolėms: Sorghum halepense (L.) Pers., Cirsium arvense (L.) Scop. ir Convolvulus arvensis L. Kitos priemonės, pavyzdžiui, trąšų rūšis, kukurūzų tarpueilių plotis ir augalų tankumas, antsėlis bei įsėlis, taip pat turėjo įtakos piktžolių biomasei kukurūzų lauke. Kukurūzų auginimas mažesniu
tarpueilių pločiu piktžolių biomasę sumažino 27,4 %, o lėtai atpalaiduojamo karbamido panaudojimas padidino augalų konkurencinę gebą. Saldžiųjų kukurūzų (Zea mays L. convar. saccharata), augintų su vikių antsėliu, pasėlyje piktžolių biomasė reikšmingai sumažėjo 
(48,5 g m-2), palyginus su variantu be antsėlio (564,3 g m-2).
PB  - Vilnius : Lithuanian Research Centre for Agriculture and Forestry
PB  - Kaunas : Vytautas Magnus University
T2  - Zemdirbyste
T1  - Integrated weed management in long-term maize cultivation
VL  - 107
IS  - 1
SP  - 33
EP  - 40
DO  - 10.13080/z-a.2020.107.005
ER  - 
@article{
author = "Simić, Milena and Dragičević, Vesna and Chachalis, Demosthenis and Dolijanović, Željko and Brankov, Milan",
year = "2020",
abstract = "The effects of different measures within maize cropping technology, aimed to suppress weeds as a part of integrated weed management (IWM) system, are analysed and evaluated in this manuscript, in line with the results of longterm experiments. For sustainable maize (Zea mays L.) production, implementation of IWM system aiming to reduce reliance on chemical weed control within Europe is a key priority. This IWM system includes all possible solutions, such as preventive, direct, biological, mechanical and alternative measures. A cropping system approach
is essential to manage weeds, utilize genetic potential of maize genotypes and reduce yield losses due to weed competition. Long-term experiments are nowadays rare, but they are an excellent and reliable method for comparing cropping systems regarding yield and reduction of weed infestation level. In the research program implemented at the Maize Research Institute Zemun Polje in Central Serbia, the effects of different cropping measures and their interactions
as a part of IWMs were studied during ten years. Maize rotations with winter wheat (Triticum aestivum L.) and soybean (Glycine max (L.) Merr.), combined with herbicide application, showed the best effect on weed biomass reduction, 92.1% and 92.2%, respectively. Winter wheat was a better preceding crop for maize than soybean, especially in combination with herbicides applied in recommended as well as in half of recommended rate. Intensification of soil tillage significantly reduced maize weed infestation, especially abundance of perennial species: Johnson grass (Sorghum halepense (L.) Pers.), Canada thistle (Cirsium arvense (L.) Scop.) and field bindweed (Convolvulus arvensis L.). Other measures, such as type of fertilizer, maize row space and crop density, cover cropping and intercropping also affected weed biomass production in maize fields. Maize growing with reduced row spacing contributed to weed biomass reduction by 27.4%, while application of slow-release urea contributed to crop competitiveness. Weed biomass in sweet maize (Zea mays L. convar. saccharata) grown with common vetch as a cover crop was significantly reduced (48.5 g m-2) compared with the treatment without a cover crop
 (564.3 g m-2)., Straipsnyje analizuojama kukurūzų įvairių auginimo priemonių ir auginimo technologijų įtaka piktžolių mažėjimui ilgalaikio eksperimento metu. Europoje tvaraus kukurūzų auginimo svarbiausias prioritetas yra integruota piktžolių kontrolės (IPK) sistema, kuria siekiama sumažinti priklausomumą nuo cheminės piktžolių kontrolės. IPK sistema apima visus įmanomus sprendimus, pavyzdžiui, prevencines tiesiogines, biologines, mechanines ir alternatyvias priemones. Augalininkystės sistemų metodas yra labai svarbus siekiant kontroliuoti piktžoles, išnaudoti kukurūzų genotipų genetinį potencialą ir sumažinti derliaus nuostolius dėl piktžolių.
Šiais laikais ilgalaikiai eksperimentai vykdomi retai, tačiau jie yra puikus ir patikimas būdas augalininkystės sistemas palyginti pagal derlių ir pasėlių piktžolėtumo mažinimą. Centrinėje Serbijoje esančiame Kukurūzų tyrimų institute Zemun Polje įgyvendinant mokslinių tyrimų programą, kelerius metus kaip IPK dalis buvo tirta įvairių agrotechnikos priemonių įtaka ir jų sąveika. Siekiant sumažinti piktžolių biomasę efektyviausia buvo paprastųjų kukurūzų (Zea mays L.) sėjomaina su žieminiais kviečiais ir sojomis kartu su herbicidų panaudojimu – atitinkamai
92,1 ir 92,2 %. Žieminiai kviečiai buvo geresnis kukurūzų priešsėlis nei sojos, ypač kartu su herbicidais, panaudojus ir rekomenduojamą normą, ir pusę rekomenduojamos jų normos. Žemės dirbimas turėjo reikšmingos įtakos kukurūzų pasėlio piktžolėtumui, ypač daugiametėms piktžolėms: Sorghum halepense (L.) Pers., Cirsium arvense (L.) Scop. ir Convolvulus arvensis L. Kitos priemonės, pavyzdžiui, trąšų rūšis, kukurūzų tarpueilių plotis ir augalų tankumas, antsėlis bei įsėlis, taip pat turėjo įtakos piktžolių biomasei kukurūzų lauke. Kukurūzų auginimas mažesniu
tarpueilių pločiu piktžolių biomasę sumažino 27,4 %, o lėtai atpalaiduojamo karbamido panaudojimas padidino augalų konkurencinę gebą. Saldžiųjų kukurūzų (Zea mays L. convar. saccharata), augintų su vikių antsėliu, pasėlyje piktžolių biomasė reikšmingai sumažėjo 
(48,5 g m-2), palyginus su variantu be antsėlio (564,3 g m-2).",
publisher = "Vilnius : Lithuanian Research Centre for Agriculture and Forestry, Kaunas : Vytautas Magnus University",
journal = "Zemdirbyste",
title = "Integrated weed management in long-term maize cultivation",
volume = "107",
number = "1",
pages = "33-40",
doi = "10.13080/z-a.2020.107.005"
}
Simić, M., Dragičević, V., Chachalis, D., Dolijanović, Ž.,& Brankov, M.. (2020). Integrated weed management in long-term maize cultivation. in Zemdirbyste
Vilnius : Lithuanian Research Centre for Agriculture and Forestry., 107(1), 33-40.
https://doi.org/10.13080/z-a.2020.107.005
Simić M, Dragičević V, Chachalis D, Dolijanović Ž, Brankov M. Integrated weed management in long-term maize cultivation. in Zemdirbyste. 2020;107(1):33-40.
doi:10.13080/z-a.2020.107.005 .
Simić, Milena, Dragičević, Vesna, Chachalis, Demosthenis, Dolijanović, Željko, Brankov, Milan, "Integrated weed management in long-term maize cultivation" in Zemdirbyste, 107, no. 1 (2020):33-40,
https://doi.org/10.13080/z-a.2020.107.005 . .
3
2

Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate

Mandić, Violeta; Đorđević, Snežana; Bijelić, Zorica; Krnjaja, Vesna; Pantelić, Vlada; Simić, Aleksandar; Dragičević, Vesna

(Basel : Multidisciplinary Digital Publishing Institute, 2020)

TY  - JOUR
AU  - Mandić, Violeta
AU  - Đorđević, Snežana
AU  - Bijelić, Zorica
AU  - Krnjaja, Vesna
AU  - Pantelić, Vlada
AU  - Simić, Aleksandar
AU  - Dragičević, Vesna
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/798
AB  - The main nitrogen (N) sources in soybean production originate from soil fixing bacteria
Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not
su cient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the e ects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha�����1) on quantitative and qualitative parameters and on rain use e ciency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two di erent growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha�����1, protein content at 90 kg N ha�����1 and oil content 0 kg N ha�����1 (control). This study suggests that proper genotype selection and application of 60 kg N ha�����1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.
PB  - Basel : Multidisciplinary Digital Publishing Institute
T2  - Agronomy
T1  - Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate
VL  - 10
IS  - 4
SP  - 535
DO  - 10.3390/agronomy10040535
ER  - 
@article{
author = "Mandić, Violeta and Đorđević, Snežana and Bijelić, Zorica and Krnjaja, Vesna and Pantelić, Vlada and Simić, Aleksandar and Dragičević, Vesna",
year = "2020",
abstract = "The main nitrogen (N) sources in soybean production originate from soil fixing bacteria
Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not
su cient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the e ects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha�����1) on quantitative and qualitative parameters and on rain use e ciency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two di erent growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha�����1, protein content at 90 kg N ha�����1 and oil content 0 kg N ha�����1 (control). This study suggests that proper genotype selection and application of 60 kg N ha�����1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute",
journal = "Agronomy",
title = "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate",
volume = "10",
number = "4",
pages = "535",
doi = "10.3390/agronomy10040535"
}
Mandić, V., Đorđević, S., Bijelić, Z., Krnjaja, V., Pantelić, V., Simić, A.,& Dragičević, V.. (2020). Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate. in Agronomy
Basel : Multidisciplinary Digital Publishing Institute., 10(4), 535.
https://doi.org/10.3390/agronomy10040535
Mandić V, Đorđević S, Bijelić Z, Krnjaja V, Pantelić V, Simić A, Dragičević V. Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate. in Agronomy. 2020;10(4):535.
doi:10.3390/agronomy10040535 .
Mandić, Violeta, Đorđević, Snežana, Bijelić, Zorica, Krnjaja, Vesna, Pantelić, Vlada, Simić, Aleksandar, Dragičević, Vesna, "Agronomic responses of soybean genotypes to starter nitrogen fertilizer rate" in Agronomy, 10, no. 4 (2020):535,
https://doi.org/10.3390/agronomy10040535 . .
1
5
6
5

First report of Aspergillus parasiticus on Barley Grain in Serbia

Nikolić, Milica; Savić, Iva; Obradović, Ana; Srdić, Jelena; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : The American Phytopathological Society (APS), 2020)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Obradović, Ana
AU  - Srdić, Jelena
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/796
AB  - Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.
PB  - St. Paul : The American Phytopathological Society (APS)
T2  - Plant Disease
T1  - First report of Aspergillus parasiticus on Barley Grain in Serbia
VL  - 104
IS  - 3
SP  - 987
DO  - 10.1094/PDIS-07-19-1364-PDN
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Obradović, Ana and Srdić, Jelena and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2020",
abstract = "Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.",
publisher = "St. Paul : The American Phytopathological Society (APS)",
journal = "Plant Disease",
title = "First report of Aspergillus parasiticus on Barley Grain in Serbia",
volume = "104",
number = "3",
pages = "987",
doi = "10.1094/PDIS-07-19-1364-PDN"
}
Nikolić, M., Savić, I., Obradović, A., Srdić, J., Stanković, G., Stevanović, M.,& Stanković, S.. (2020). First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease
St. Paul : The American Phytopathological Society (APS)., 104(3), 987.
https://doi.org/10.1094/PDIS-07-19-1364-PDN
Nikolić M, Savić I, Obradović A, Srdić J, Stanković G, Stevanović M, Stanković S. First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease. 2020;104(3):987.
doi:10.1094/PDIS-07-19-1364-PDN .
Nikolić, Milica, Savić, Iva, Obradović, Ana, Srdić, Jelena, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First report of Aspergillus parasiticus on Barley Grain in Serbia" in Plant Disease, 104, no. 3 (2020):987,
https://doi.org/10.1094/PDIS-07-19-1364-PDN . .