The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques.

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The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (en)
Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (sr)
Interakcije membrana sa unutarćelijskim i apoplastičnim prostorom: izučavanja bioenergetike i singalizacije koristeći biofizičke i biohemijske metode (sr_RS)
Authors

Publications

Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives

Žilić, Slađana; Dodig, Dejan; Vančetović, Jelena; Grčić, Nikola; Perić, Vesna; Titan, Primož; Maksimović, Vuk

(Olsztyn : Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, 2019)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Dodig, Dejan
AU  - Vančetović, Jelena
AU  - Grčić, Nikola
AU  - Perić, Vesna
AU  - Titan, Primož
AU  - Maksimović, Vuk
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/769
AB  - Colored grains are rich sources of anthocyanins that could play an important role in the prevention of various diseases associated with oxidative
stress. Bearing in mind that cereals are widely grown crops, anthocyanins-rich colored grains could be used as a functional food ingredient that
provides health benefi ts to a large part of human world’s population. This study investigated composition and content of anthocyanins in the grain
of blue popping maize, deep purple maize, purple wheat, and black soybean. The relationship of acylated and non-acylated forms before and after
alkaline hydrolysis of anthocyanin extracts has been studied as well. Deep purple maize had the highest content of total anthocyanins reaching as
much as 4988.90 mg CGE/kg d.m. Ten anthocyanins were identifi ed in blue popping maize, of which two are isomers of cyanidin-3-(malonylglucoside)
and three of cyanidin-3-(dimalonyl-β-glucoside). Seven, nine, and three anthocyanins have been identifi ed in the deep purple maize, purple wheat,
and black soybean, respectively. Cyanidin derivatives were predominant and their acylated forms accounted for about 98, 29, 71, and 0% of the total
anthocyanins content in the grains, respectively. According to the study, acylated derivatives were completely degraded under the effect of highly alkaline
pH. However, at the beginning of their degradation they were transformed to their non-acylated parents.
The results could be useful to better understanding of the nature of anthocyanin in colored grains and, in that regard, their use for the derivation
of food products with functional potential, as well as of natural dyes and pharmaceutical ingredients.
PB  - Olsztyn : Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives
VL  - 69
IS  - 2
SP  - 137
EP  - 146
DO  - 10.31883/pjfns-2019-105100
ER  - 
@article{
author = "Žilić, Slađana and Dodig, Dejan and Vančetović, Jelena and Grčić, Nikola and Perić, Vesna and Titan, Primož and Maksimović, Vuk",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/769",
abstract = "Colored grains are rich sources of anthocyanins that could play an important role in the prevention of various diseases associated with oxidative
stress. Bearing in mind that cereals are widely grown crops, anthocyanins-rich colored grains could be used as a functional food ingredient that
provides health benefi ts to a large part of human world’s population. This study investigated composition and content of anthocyanins in the grain
of blue popping maize, deep purple maize, purple wheat, and black soybean. The relationship of acylated and non-acylated forms before and after
alkaline hydrolysis of anthocyanin extracts has been studied as well. Deep purple maize had the highest content of total anthocyanins reaching as
much as 4988.90 mg CGE/kg d.m. Ten anthocyanins were identifi ed in blue popping maize, of which two are isomers of cyanidin-3-(malonylglucoside)
and three of cyanidin-3-(dimalonyl-β-glucoside). Seven, nine, and three anthocyanins have been identifi ed in the deep purple maize, purple wheat,
and black soybean, respectively. Cyanidin derivatives were predominant and their acylated forms accounted for about 98, 29, 71, and 0% of the total
anthocyanins content in the grains, respectively. According to the study, acylated derivatives were completely degraded under the effect of highly alkaline
pH. However, at the beginning of their degradation they were transformed to their non-acylated parents.
The results could be useful to better understanding of the nature of anthocyanin in colored grains and, in that regard, their use for the derivation
of food products with functional potential, as well as of natural dyes and pharmaceutical ingredients.",
publisher = "Olsztyn : Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives",
volume = "69",
number = "2",
pages = "137-146",
doi = "10.31883/pjfns-2019-105100"
}
Žilić, S., Dodig, D., Vančetović, J., Grčić, N., Perić, V., Titan, P.,& Maksimović, V. (2019). Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives.
Polish Journal of Food and Nutrition Sciences
Olsztyn : Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences., 69(2), 137-146.
https://doi.org/10.31883/pjfns-2019-105100
Žilić S, Dodig D, Vančetović J, Grčić N, Perić V, Titan P, Maksimović V. Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives. Polish Journal of Food and Nutrition Sciences. 2019;69(2):137-146
Žilić Slađana, Dodig Dejan, Vančetović Jelena, Grčić Nikola, Perić Vesna, Titan Primož, Maksimović Vuk, "Composition of Anthocyanins in Colored Grains and the Relationship of Their Non-Acylated and Acylated Derivatives" Polish Journal of Food and Nutrition Sciences, 69, no. 2 (2019):137-146,
https://doi.org/10.31883/pjfns-2019-105100 .
8
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Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Vuletić, Mirjana; Marković, Ksenija; Kravić, Natalija

(Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid, 2016)

TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
AU  - Kravić, Natalija
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/618
AB  - Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.
PB  - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
T2  - Spanish Journal of Agricultural Research
T1  - Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources
VL  - 14
IS  - 4
DO  - 10.5424/sjar/2016144-8305
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Vuletić, Mirjana and Marković, Ksenija and Kravić, Natalija",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/618",
abstract = "Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.",
publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid",
journal = "Spanish Journal of Agricultural Research",
title = "Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources",
volume = "14",
number = "4",
doi = "10.5424/sjar/2016144-8305"
}
Hadži-Tašković-Šukalović, V., Vučinić, Ž., Vuletić, M., Marković, K.,& Kravić, N. (2016). Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources.
Spanish Journal of Agricultural Research
Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 14(4).
https://doi.org/10.5424/sjar/2016144-8305
Hadži-Tašković-Šukalović V, Vučinić Ž, Vuletić M, Marković K, Kravić N. Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources. Spanish Journal of Agricultural Research. 2016;14(4)
Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Vuletić Mirjana, Marković Ksenija, Kravić Natalija, "Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources" Spanish Journal of Agricultural Research, 14, no. 4 (2016),
https://doi.org/10.5424/sjar/2016144-8305 .
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Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L): Comparison with medicinal herbs

Žilić, Slađana; Janković, Marijana; Vančetović, Jelena; Basić, Zorica; Maksimović, Vuk

(Academic Press Ltd- Elsevier Science Ltd, London, 2016)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Janković, Marijana
AU  - Vančetović, Jelena
AU  - Basić, Zorica
AU  - Maksimović, Vuk
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/623
AB  - Yellow, green, pinky and purple colored corn silk were harvested 5 and 25 days after emergence and compared with six medicinal herbs for total phenolics, flavonoids, anthocyanins, proanthocyanidins, chlorophylls, as well as antioxidant activity. The phenolic acid, flavonoid and mineral matter profiles of corn silks were also determined. Corn silks at the silking stage are much more suitable for use as a natural source of phenolic compounds than silks at the R4 dough stage. The content of total phenolic and flavonoid compounds in fresh silk was higher by about 2- to 4-fold than in the silk at the R4 dough stage and by about 1.2- to 2.6-fold than in the most investigated herbs. The most abundant phenolic compounds were hydroxycinnamic acid ester and luteolin derivative i.e. 3-O-caffeoylquinic acid and maysin, respectively. A high potassium content and antioxidant activity of silk are associated with its health benefit properties.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Cereal Science
T1  - Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L): Comparison with medicinal herbs
VL  - 69
SP  - 363
EP  - 370
DO  - 10.1016/j.jcs.2016.05.003
ER  - 
@article{
author = "Žilić, Slađana and Janković, Marijana and Vančetović, Jelena and Basić, Zorica and Maksimović, Vuk",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/623",
abstract = "Yellow, green, pinky and purple colored corn silk were harvested 5 and 25 days after emergence and compared with six medicinal herbs for total phenolics, flavonoids, anthocyanins, proanthocyanidins, chlorophylls, as well as antioxidant activity. The phenolic acid, flavonoid and mineral matter profiles of corn silks were also determined. Corn silks at the silking stage are much more suitable for use as a natural source of phenolic compounds than silks at the R4 dough stage. The content of total phenolic and flavonoid compounds in fresh silk was higher by about 2- to 4-fold than in the silk at the R4 dough stage and by about 1.2- to 2.6-fold than in the most investigated herbs. The most abundant phenolic compounds were hydroxycinnamic acid ester and luteolin derivative i.e. 3-O-caffeoylquinic acid and maysin, respectively. A high potassium content and antioxidant activity of silk are associated with its health benefit properties.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Cereal Science",
title = "Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L): Comparison with medicinal herbs",
volume = "69",
pages = "363-370",
doi = "10.1016/j.jcs.2016.05.003"
}
Žilić, S., Janković, M., Vančetović, J., Basić, Z.,& Maksimović, V. (2016). Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L): Comparison with medicinal herbs.
Journal of Cereal Science
Academic Press Ltd- Elsevier Science Ltd, London., 69, 363-370.
https://doi.org/10.1016/j.jcs.2016.05.003
Žilić S, Janković M, Vančetović J, Basić Z, Maksimović V. Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L): Comparison with medicinal herbs. Journal of Cereal Science. 2016;69:363-370
Žilić Slađana, Janković Marijana, Vančetović Jelena, Basić Zorica, Maksimović Vuk, "Antioxidant activity, phenolic profile, chlorophyll and mineral matter content of corn silk (Zea mays L): Comparison with medicinal herbs" Journal of Cereal Science, 69 (2016):363-370,
https://doi.org/10.1016/j.jcs.2016.05.003 .
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19
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Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding

Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Vuletić, Mirjana; Marković, Ksenija; Cvetic-Antić, Tijana

(Springer Wien, Wien, 2015)

TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
AU  - Cvetic-Antić, Tijana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/591
AB  - Comparative biochemical characterization of class III peroxidase activity tightly bound to the cell walls of maize roots was performed. Ionically bound proteins were solubilized from isolated walls by salt washing, and the remaining covalently bound peroxidases were released, either by enzymatic digestion or by a novel alkaline extraction procedure that released covalently bound alkali-resistant peroxidase enzyme. Solubilized fractions, as well as the salt-washed cell wall fragments containing covalently bound proteins, were analyzed for peroxidase activity. Peroxidative and oxidative activities indicated that peroxidase enzymes were predominately associated with walls by ionic interactions, and this fraction differs from the covalently bound one according to molecular weight, isozyme patterns, and biochemical parameters. The effect of covalent binding was evaluated by comparison of the catalytic properties of the enzyme bound to the salt-washed cell wall fragments with the corresponding solubilized and released enzyme. Higher thermal stability, improved resistance to KCN, increased susceptibility to H2O2, stimulated capacity of wall-bound enzyme to oxidize indole-3-acetic acid (IAA) as well as the difference in kinetic parameters between free and bound enzymes point to conformational changes due to covalent binding. Differences in biochemical properties of ionically and covalently bound peroxidases, as well as the modulation of the enzyme properties as a result of covalent binding to the walls, indicate that these two fractions of apoplastic peroxidases play different roles.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding
VL  - 252
IS  - 1
SP  - 335
EP  - 343
DO  - 10.1007/s00709-014-0684-2
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Vuletić, Mirjana and Marković, Ksenija and Cvetic-Antić, Tijana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/591",
abstract = "Comparative biochemical characterization of class III peroxidase activity tightly bound to the cell walls of maize roots was performed. Ionically bound proteins were solubilized from isolated walls by salt washing, and the remaining covalently bound peroxidases were released, either by enzymatic digestion or by a novel alkaline extraction procedure that released covalently bound alkali-resistant peroxidase enzyme. Solubilized fractions, as well as the salt-washed cell wall fragments containing covalently bound proteins, were analyzed for peroxidase activity. Peroxidative and oxidative activities indicated that peroxidase enzymes were predominately associated with walls by ionic interactions, and this fraction differs from the covalently bound one according to molecular weight, isozyme patterns, and biochemical parameters. The effect of covalent binding was evaluated by comparison of the catalytic properties of the enzyme bound to the salt-washed cell wall fragments with the corresponding solubilized and released enzyme. Higher thermal stability, improved resistance to KCN, increased susceptibility to H2O2, stimulated capacity of wall-bound enzyme to oxidize indole-3-acetic acid (IAA) as well as the difference in kinetic parameters between free and bound enzymes point to conformational changes due to covalent binding. Differences in biochemical properties of ionically and covalently bound peroxidases, as well as the modulation of the enzyme properties as a result of covalent binding to the walls, indicate that these two fractions of apoplastic peroxidases play different roles.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding",
volume = "252",
number = "1",
pages = "335-343",
doi = "10.1007/s00709-014-0684-2"
}
Hadži-Tašković-Šukalović, V., Vučinić, Ž., Vuletić, M., Marković, K.,& Cvetic-Antić, T. (2015). Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding.
Protoplasma
Springer Wien, Wien., 252(1), 335-343.
https://doi.org/10.1007/s00709-014-0684-2
Hadži-Tašković-Šukalović V, Vučinić Ž, Vuletić M, Marković K, Cvetic-Antić T. Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding. Protoplasma. 2015;252(1):335-343
Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Vuletić Mirjana, Marković Ksenija, Cvetic-Antić Tijana, "Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding" Protoplasma, 252, no. 1 (2015):335-343,
https://doi.org/10.1007/s00709-014-0684-2 .
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6

Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen

Žilić, Slađana; Janković, Marijana; Vančetović, Jelena; Maksimović, Vuk

(Elsevier Science Bv, Amsterdam, 2014)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Janković, Marijana
AU  - Vančetović, Jelena
AU  - Maksimović, Vuk
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/538
AB  - The nutritional composition, the phenolic profiles and antioxidant capacity of the floral pollen from maize genotypes were evaluated. In addition, the antioxidant capacity, browning and wavelength spectra of melanoidins were investigated in the pollen samples stored at 4 degrees C for 7 days, dried at 40 degrees C for 6 h and exposed to a temperature of 100 degrees C during 12 h. The results showed that different maize pollen samples had diverse nutritional composition, antioxidant capacity and phenolic compounds were a major contributor to their antioxidant activities. Sweet maize pollen with the highest content of total phenolics and flavonoids had the highest antioxidant capacity (104.38 mmol trolox eq/kg). Quercetin diglycoside was the most abundant flavonoid in all pollen samples. Floral maize pollen could be used as a functional food ingredients, and dietary supplement with therapeutic effects. However, pollen is very susceptible to processes of Maillard reaction and phenolics oxidation and these factors must be considered when considering its use.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen
VL  - 10
SP  - 65
EP  - 74
DO  - 10.1016/j.jff.2014.05.007
ER  - 
@article{
author = "Žilić, Slađana and Janković, Marijana and Vančetović, Jelena and Maksimović, Vuk",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/538",
abstract = "The nutritional composition, the phenolic profiles and antioxidant capacity of the floral pollen from maize genotypes were evaluated. In addition, the antioxidant capacity, browning and wavelength spectra of melanoidins were investigated in the pollen samples stored at 4 degrees C for 7 days, dried at 40 degrees C for 6 h and exposed to a temperature of 100 degrees C during 12 h. The results showed that different maize pollen samples had diverse nutritional composition, antioxidant capacity and phenolic compounds were a major contributor to their antioxidant activities. Sweet maize pollen with the highest content of total phenolics and flavonoids had the highest antioxidant capacity (104.38 mmol trolox eq/kg). Quercetin diglycoside was the most abundant flavonoid in all pollen samples. Floral maize pollen could be used as a functional food ingredients, and dietary supplement with therapeutic effects. However, pollen is very susceptible to processes of Maillard reaction and phenolics oxidation and these factors must be considered when considering its use.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen",
volume = "10",
pages = "65-74",
doi = "10.1016/j.jff.2014.05.007"
}
Žilić, S., Janković, M., Vančetović, J.,& Maksimović, V. (2014). Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen.
Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 10, 65-74.
https://doi.org/10.1016/j.jff.2014.05.007
Žilić S, Janković M, Vančetović J, Maksimović V. Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen. Journal of Functional Foods. 2014;10:65-74
Žilić Slađana, Janković Marijana, Vančetović Jelena, Maksimović Vuk, "Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen" Journal of Functional Foods, 10 (2014):65-74,
https://doi.org/10.1016/j.jff.2014.05.007 .
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Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress

Vuletić, Mirjana; Marković, Ksenija; Kravić, Natalija; Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Maksimović, Vuk

(Wiley-Blackwell, Hoboken, 2014)

TY  - JOUR
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
AU  - Kravić, Natalija
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/551
AB  - An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Biology
T1  - Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress
VL  - 16
IS  - 1
SP  - 88
EP  - 96
DO  - 10.1111/plb.12017
ER  - 
@article{
author = "Vuletić, Mirjana and Marković, Ksenija and Kravić, Natalija and Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Maksimović, Vuk",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/551",
abstract = "An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Biology",
title = "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress",
volume = "16",
number = "1",
pages = "88-96",
doi = "10.1111/plb.12017"
}
Vuletić, M., Marković, K., Kravić, N., Hadži-Tašković-Šukalović, V., Vučinić, Ž.,& Maksimović, V. (2014). Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress.
Plant Biology
Wiley-Blackwell, Hoboken., 16(1), 88-96.
https://doi.org/10.1111/plb.12017
Vuletić M, Marković K, Kravić N, Hadži-Tašković-Šukalović V, Vučinić Ž, Maksimović V. Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress. Plant Biology. 2014;16(1):88-96
Vuletić Mirjana, Marković Ksenija, Kravić Natalija, Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Maksimović Vuk, "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress" Plant Biology, 16, no. 1 (2014):88-96,
https://doi.org/10.1111/plb.12017 .
16
16
20

Cell wall-associated malate dehydrogenase activity from maize roots

Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Vuletić, Mirjana; Marković, Ksenija

(Elsevier Ireland Ltd, Clare, 2011)

TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/373
AB  - Isolated cell walls from maize (Zea mays L) roots exhibited ionically and covalently bound NAD-specific malate dehydrogenase activity. The enzyme catalyses a rapid reduction of oxaloacetate and much slower oxidation of malate. The kinetic and regulatory properties of the cell wall enzyme solubilized with 1 M NaCl were different from those published for soluble, mitochondrial or plasma membrane malate dehydrogenase with respect to their ATP, Pi, and pH dependence. Isoelectric focusing of ionically-bound proteins and specific staining for malate dehydrogenase revealed characteristic isoforms present in cell wall isolate, different from those present in plasma membranes and crude homogenate. Much greater activity of cell wall-associated malate dehydrogenase was detected in the intensively growing lateral roots compared to primary root with decreased growth rates. Presence of Zn2+ and Cu2+ in the assay medium inhibited the activity of the wall-associated malate dehydrogenase. Exposure of maize plants to excess concentrations of Zn2+ and Cu2+ in the hydroponic solution inhibited lateral root growth, decreased malate dehydrogenase activity and changed isoform profiles. The results presented show that cell wall malate dehydrogenase is truly a wall-bound enzyme, and not an artefact of cytoplasmic contamination, involved in the developmental processes, and detoxification of heavy metals.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Cell wall-associated malate dehydrogenase activity from maize roots
VL  - 181
IS  - 4
SP  - 465
EP  - 470
DO  - 10.1016/j.plantsci.2011.07.007
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Vuletić, Mirjana and Marković, Ksenija",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/373",
abstract = "Isolated cell walls from maize (Zea mays L) roots exhibited ionically and covalently bound NAD-specific malate dehydrogenase activity. The enzyme catalyses a rapid reduction of oxaloacetate and much slower oxidation of malate. The kinetic and regulatory properties of the cell wall enzyme solubilized with 1 M NaCl were different from those published for soluble, mitochondrial or plasma membrane malate dehydrogenase with respect to their ATP, Pi, and pH dependence. Isoelectric focusing of ionically-bound proteins and specific staining for malate dehydrogenase revealed characteristic isoforms present in cell wall isolate, different from those present in plasma membranes and crude homogenate. Much greater activity of cell wall-associated malate dehydrogenase was detected in the intensively growing lateral roots compared to primary root with decreased growth rates. Presence of Zn2+ and Cu2+ in the assay medium inhibited the activity of the wall-associated malate dehydrogenase. Exposure of maize plants to excess concentrations of Zn2+ and Cu2+ in the hydroponic solution inhibited lateral root growth, decreased malate dehydrogenase activity and changed isoform profiles. The results presented show that cell wall malate dehydrogenase is truly a wall-bound enzyme, and not an artefact of cytoplasmic contamination, involved in the developmental processes, and detoxification of heavy metals.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Cell wall-associated malate dehydrogenase activity from maize roots",
volume = "181",
number = "4",
pages = "465-470",
doi = "10.1016/j.plantsci.2011.07.007"
}
Hadži-Tašković-Šukalović, V., Vučinić, Ž., Vuletić, M.,& Marković, K. (2011). Cell wall-associated malate dehydrogenase activity from maize roots.
Plant Science
Elsevier Ireland Ltd, Clare., 181(4), 465-470.
https://doi.org/10.1016/j.plantsci.2011.07.007
Hadži-Tašković-Šukalović V, Vučinić Ž, Vuletić M, Marković K. Cell wall-associated malate dehydrogenase activity from maize roots. Plant Science. 2011;181(4):465-470
Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Vuletić Mirjana, Marković Ksenija, "Cell wall-associated malate dehydrogenase activity from maize roots" Plant Science, 181, no. 4 (2011):465-470,
https://doi.org/10.1016/j.plantsci.2011.07.007 .
19
23
23

Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants

Žilić, Slađana; Dodig, Dejan; Hadži-Tašković-Šukalović, Vesna; Maksimović, Vuk; Maksimović, Milan; Basić, Zorica

(Academic Press Ltd- Elsevier Science Ltd, London, 2011)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Dodig, Dejan
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Maksimović, Vuk
AU  - Maksimović, Milan
AU  - Basić, Zorica
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/400
AB  - In this study, the content of soluble, free forms of phenolic compounds (total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids), as well as the content of carotenoids and tocopherols, were determined in whole grains of bread and durum wheat, rye, hull-less barley and hull-less oat, each represented with four genotypes. Antioxidant activity was evaluated as radical scavenging activity with DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, as well as by hydrogen transfer reaction (reduction power) based on the reduction of Fe(3+). Generally, a considerable variation in antioxidant activities and phytochemical contents was observed between the cereals. Remarkably higher DPPH radical scavenging ability and reducing power were detected in hull-less barley, followed by rye and hull-less oat and durum and bread wheat, indicating that small grain species have different major antioxidants with different properties. Hull-less barley had the highest content of total free phenols, flavonoids, PVPP bound phenolics and contained flavan-3-ols, not found in other species. Hull-less oat had the highest content of tocopherols, very high content of yellow pigments and PVPP bound phenolics. Ferulic acid was the major free phenolic acid in small grain cereals tested. The relationship between the content of soluble phenols, as well as reducing power and DPPH scavenging activity are also considered.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Cereal Science
T1  - Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants
VL  - 54
IS  - 3
SP  - 417
EP  - 424
DO  - 10.1016/j.jcs.2011.08.006
ER  - 
@article{
author = "Žilić, Slađana and Dodig, Dejan and Hadži-Tašković-Šukalović, Vesna and Maksimović, Vuk and Maksimović, Milan and Basić, Zorica",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/400",
abstract = "In this study, the content of soluble, free forms of phenolic compounds (total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids), as well as the content of carotenoids and tocopherols, were determined in whole grains of bread and durum wheat, rye, hull-less barley and hull-less oat, each represented with four genotypes. Antioxidant activity was evaluated as radical scavenging activity with DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, as well as by hydrogen transfer reaction (reduction power) based on the reduction of Fe(3+). Generally, a considerable variation in antioxidant activities and phytochemical contents was observed between the cereals. Remarkably higher DPPH radical scavenging ability and reducing power were detected in hull-less barley, followed by rye and hull-less oat and durum and bread wheat, indicating that small grain species have different major antioxidants with different properties. Hull-less barley had the highest content of total free phenols, flavonoids, PVPP bound phenolics and contained flavan-3-ols, not found in other species. Hull-less oat had the highest content of tocopherols, very high content of yellow pigments and PVPP bound phenolics. Ferulic acid was the major free phenolic acid in small grain cereals tested. The relationship between the content of soluble phenols, as well as reducing power and DPPH scavenging activity are also considered.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Cereal Science",
title = "Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants",
volume = "54",
number = "3",
pages = "417-424",
doi = "10.1016/j.jcs.2011.08.006"
}
Žilić, S., Dodig, D., Hadži-Tašković-Šukalović, V., Maksimović, V., Maksimović, M.,& Basić, Z. (2011). Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants.
Journal of Cereal Science
Academic Press Ltd- Elsevier Science Ltd, London., 54(3), 417-424.
https://doi.org/10.1016/j.jcs.2011.08.006
Žilić S, Dodig D, Hadži-Tašković-Šukalović V, Maksimović V, Maksimović M, Basić Z. Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants. Journal of Cereal Science. 2011;54(3):417-424
Žilić Slađana, Dodig Dejan, Hadži-Tašković-Šukalović Vesna, Maksimović Vuk, Maksimović Milan, Basić Zorica, "Antioxidant activity of small grain cereals caused by phenolics and lipid soluble antioxidants" Journal of Cereal Science, 54, no. 3 (2011):417-424,
https://doi.org/10.1016/j.jcs.2011.08.006 .
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