Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200040 (Maize Research Institute 'Zemun Polje', Belgrade-Zemun)

Link to this page

info:eu-repo/grantAgreement/MESTD/inst-2020/200040/RS//

Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200040 (Maize Research Institute 'Zemun Polje', Belgrade-Zemun) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200040 (Institut za kukuruz 'Zemun polje', Beograd-Zemun) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200040 (Институт за кукуруз 'Земун поље', Београд-Земун) (sr)
Authors

Publications

Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Perić, Vesna; Srdić, Jelena; Vasić, Marko G.

(Novi Sad : National Society of Processing and Energy in Agriculture, 2022-03-17)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Perić, Vesna
AU  - Srdić, Jelena
AU  - Vasić, Marko G.
PY  - 2022-03-17
UR  - http://rik.mrizp.rs/handle/123456789/847
AB  - Black  soybean  seed  coat  has  a  considerable  content  of  anthocyanins  which  can  providea  positive  effect  on  the  health  of  the  consumers through food products enriched with the extracts of these valuable bioactive compounds. Nevertheless, the use of soybean seed  coat,  a  by-product  from  soybean  processing,  additionally  valorizes  this  raw  material.The  possibility  of  enrichment  of  sweet maize  grains  with  anthocyanin  extract  was  investigated.  Several  procedures  with  acetic  acid  were  applied  in  the  experiments  of  anthocyanins  extraction  from  black  soybean  seed  coat.  During  the  marination  process,  the  grains  were  stained,  changing  their  standard yellow color to a crimson red. Since the brines with the addition of citric acid became cloudy (opalescent) after some time, lactic  acid  was  used  as  an  anthocyanin  stabilizer  in  the  continuation  of  the  research.  Depending  on  the  extraction  conditions,  the spectrophotometrically determined content of total anthocyanins in the solution varied from 3541.90 to 5387.70 μg CGE/g d.m., and in the marinated maize grain between 179.89 and 286.05 μg CGE/g d.m. After seven days, the total anthocyanin content in the grain did not increase significantly, so this aging period was selected as optimal for marinating maize products.
AB  - Semenjača  crne  soje  ima  značajan  sadržaj  antocijana,  koji  mogu  pozitivno  uticati  na  zdravlje  potrošača  kroz  prehrambene  proizvode  obogaćene  ekstraktima  ovih  vrednih  bioaktivnih  jedinjenja.  Istovremeno,  upotrebom  sojine  semenjače,  sporednog  proizvoda prerade soje, dodatno se valorizuje ova sirovinu. Ispitivana je mogućnost obogaćivanja zrna kukuruza šećerca ekstraktom antocijana.  U  eksperimentima  ekstrakcije  antocijana  iz  semenjače  crne  soje  primenjeno  je  nekoliko  postupaka  sa  sirćetnom kiselinom.  Tokom  procesa  mariniranja,  zrna  su  promenila  boju,  menjajući  standardnu  žutu  boju  u  tamnocrvenu.  S  obzirom  da  su  posle izvesnog vremena nalivi za mariniranje sa dodatkom limunske kiseline postali zamućeni (opalescentni), u nastavku istraživanja je  korišćena  mlečna  kiselina  kao  stabilizator  antocijana.  U  zavisnosti  od  uslova  ekstrakcije,  spektrofotometrijski  utvrđen  sadržaj  ukupnih  antocijana  u  rastvoru  varirao  je  od  3541,90  do  5387,70  mg  CGE/g  d.m.,  a  u  zrnu  mariniranog  kukuruza  između  179,89  i  286,05  mg  CGE/g.  Posle  sedam  dana,  ukupan  sadržaj  antocijana  u  zrnu  nije  značajno  povećan,  pa  je  ovaj  period  odležavanja  izabran kao optimalan za mariniranje proizvoda od kukuruza.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food
T1  - Optimizacija procesa ekstrakcije antocijana iz semenjače crne soje za pripremu funkcionalne hrane od kukuruza
VL  - 26
IS  - 1
SP  - 19
EP  - 22
DO  - 10.5937/jpea%v-36635
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Perić, Vesna and Srdić, Jelena and Vasić, Marko G.",
year = "2022-03-17",
abstract = "Black  soybean  seed  coat  has  a  considerable  content  of  anthocyanins  which  can  providea  positive  effect  on  the  health  of  the  consumers through food products enriched with the extracts of these valuable bioactive compounds. Nevertheless, the use of soybean seed  coat,  a  by-product  from  soybean  processing,  additionally  valorizes  this  raw  material.The  possibility  of  enrichment  of  sweet maize  grains  with  anthocyanin  extract  was  investigated.  Several  procedures  with  acetic  acid  were  applied  in  the  experiments  of  anthocyanins  extraction  from  black  soybean  seed  coat.  During  the  marination  process,  the  grains  were  stained,  changing  their  standard yellow color to a crimson red. Since the brines with the addition of citric acid became cloudy (opalescent) after some time, lactic  acid  was  used  as  an  anthocyanin  stabilizer  in  the  continuation  of  the  research.  Depending  on  the  extraction  conditions,  the spectrophotometrically determined content of total anthocyanins in the solution varied from 3541.90 to 5387.70 μg CGE/g d.m., and in the marinated maize grain between 179.89 and 286.05 μg CGE/g d.m. After seven days, the total anthocyanin content in the grain did not increase significantly, so this aging period was selected as optimal for marinating maize products., Semenjača  crne  soje  ima  značajan  sadržaj  antocijana,  koji  mogu  pozitivno  uticati  na  zdravlje  potrošača  kroz  prehrambene  proizvode  obogaćene  ekstraktima  ovih  vrednih  bioaktivnih  jedinjenja.  Istovremeno,  upotrebom  sojine  semenjače,  sporednog  proizvoda prerade soje, dodatno se valorizuje ova sirovinu. Ispitivana je mogućnost obogaćivanja zrna kukuruza šećerca ekstraktom antocijana.  U  eksperimentima  ekstrakcije  antocijana  iz  semenjače  crne  soje  primenjeno  je  nekoliko  postupaka  sa  sirćetnom kiselinom.  Tokom  procesa  mariniranja,  zrna  su  promenila  boju,  menjajući  standardnu  žutu  boju  u  tamnocrvenu.  S  obzirom  da  su  posle izvesnog vremena nalivi za mariniranje sa dodatkom limunske kiseline postali zamućeni (opalescentni), u nastavku istraživanja je  korišćena  mlečna  kiselina  kao  stabilizator  antocijana.  U  zavisnosti  od  uslova  ekstrakcije,  spektrofotometrijski  utvrđen  sadržaj  ukupnih  antocijana  u  rastvoru  varirao  je  od  3541,90  do  5387,70  mg  CGE/g  d.m.,  a  u  zrnu  mariniranog  kukuruza  između  179,89  i  286,05  mg  CGE/g.  Posle  sedam  dana,  ukupan  sadržaj  antocijana  u  zrnu  nije  značajno  povećan,  pa  je  ovaj  period  odležavanja  izabran kao optimalan za mariniranje proizvoda od kukuruza.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food, Optimizacija procesa ekstrakcije antocijana iz semenjače crne soje za pripremu funkcionalne hrane od kukuruza",
volume = "26",
number = "1",
pages = "19-22",
doi = "10.5937/jpea%v-36635"
}
Nikolić, V., Žilić, S., Simić, M., Perić, V., Srdić, J.,& Vasić, M. G.. (2022-03-17). Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 26(1), 19-22.
https://doi.org/10.5937/jpea%v-36635
Nikolić V, Žilić S, Simić M, Perić V, Srdić J, Vasić MG. Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food. in Journal on Processing and Energy in Agriculture. 2022;26(1):19-22.
doi:10.5937/jpea%v-36635 .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Perić, Vesna, Srdić, Jelena, Vasić, Marko G., "Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food" in Journal on Processing and Energy in Agriculture, 26, no. 1 (2022-03-17):19-22,
https://doi.org/10.5937/jpea%v-36635 . .

The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research

Nikolić, Valentina; Babić, Vojka; Kravić, Natalija; Filipović, Milomir; Žilić, Slađana; Simić, Marijana; Radosavljević, Milica

(Belgrade : Serbian Association of Plant Breedings and Seed Producers, 2022-01-21)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Babić, Vojka
AU  - Kravić, Natalija
AU  - Filipović, Milomir
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Radosavljević, Milica
PY  - 2022-01-21
UR  - http://rik.mrizp.rs/handle/123456789/849
AB  - In the process of hybrid maize breeding, the greatest attention is directed towards increasing the level and stability of the yield, while grain quality is of secondary importance. The very
principles of hybrid breeding, as well as the economic aspects and competition between different breeding companies, have led to an enormous narrowing of the genetic basis of commercial
maize hybrids. Concurrently, there is usually a small number of often related hybrids of similar
technological and nutritional quality present on the market. Six best-selling ZP maize hybrids
in the previous ten years were chosen as the subject of this study. In the period from 2009-2018,
both the yield components and parameters of technological and nutritional quality of the grain
were studied. It was found that the examined parameters varied a lot in different years of production and that some parameters were more influenced by genotype (number of kernel rows per ear,
portion of pericarp, and crude fiber content) while others were more influenced by the environment (kernel weight per ear, total protein, starch, and oil content, respectively). The amount and
the distribution of precipitation during the vegetative period affected not only the yield components i.e. grain yield but also the technological qualitz parametres and nutritional properties of
the grain. Based on the observed parental components, it is evident that they are related hybrids
that, despite the high genotype by environment interactions, consequently have similar parameters of technological quality and nutritional composition. The imperative is to develop special
breeding programs aiming to create both the starting material and parental components to provide hybrids of improved technological quality and nutritional properties on the market. In that
way, the needs of the processing industry for raw materials of certain characteristics can be met.
AB  - U procesu hibridnog oplemenjivanja kukuruza, najviše pažnje se posvećuje povećanju nivoa
i stabilnosti prinosa, dok je kvalitet zrna od sekundarnog značaja. Sami principi hibridnog oplemenjivanja, kao i ekonomski aspekti i konkurencija između različitih oplemenjivačkih kuća,
doveli su do enormnog suženja genetičke osnove komercijalnih hibrida kukuruza. Na tržištu je
istovremeno najčešće prisutan mali broj, često srodnih hibrida sličnog tehnološkog i nutritivnog
kvaliteta. Za ovo istraživanje je odabrano šest ZP hibrida kukuruza najprodavanijih u prethodnih
deset godina. U periodu od 2009-2018. godine, praćene su, kako komponente prinosa, tako i
parametri tehnološkog i nutritivnog kvaliteta zrna. Utvrđeno je da su ispitivani parametri veoma
varirali u različitim godinama gajenja, kao i da su neki parametri bili pod većim uticajem genotipa (broj redova zrna, udeo perikarpa i sirove celuloze), a neki pod većim uticajem spoljašnje sredine (masa zrna po klipu, udeo ukupnih proteina, skroba i ulja, respektivno). Količina i raspored
padavina u toku vegetativnog perioda su uticali ne samo na komponente prinosa, odnosno na
prinos zrna, već i na parametre tehnološkog kvaliteta i nutritivna svojstva. Na osnovu posmatranih roditeljskih komponenata, evidentno je da se radi o srodnim hibridima koji, bez obzira na
visoke interakcije između genotipa i spoljašnje sredine, posledično imaju slične parametre tehnološkog kvaliteta kao i nutritivni sastav. Neophodno je razvijati posebne oplemenjivačke programe
koji imaju za cilj stvaranje kako početnog materijala, tako i roditeljskih komponenata, kako bi
se na tržištu obezbedili hibridi poboljšanog tehnološkog kvaliteta i nutritivnih svojstava i na taj
način zadovoljile potrebe prerađivačke industrije za sirovinama određenih karakteristika.
PB  - Belgrade : Serbian Association of Plant Breedings and Seed Producers
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research
T1  - Varijabilnost i međuzavisnost osnovnih parametara tehnološkog kvaliteta hibrida kukuruza u dugoročnim istraživanjima
VL  - 27
IS  - 2
SP  - 21
EP  - 33
DO  - 10.5937/SelSem2102021N
ER  - 
@article{
author = "Nikolić, Valentina and Babić, Vojka and Kravić, Natalija and Filipović, Milomir and Žilić, Slađana and Simić, Marijana and Radosavljević, Milica",
year = "2022-01-21",
abstract = "In the process of hybrid maize breeding, the greatest attention is directed towards increasing the level and stability of the yield, while grain quality is of secondary importance. The very
principles of hybrid breeding, as well as the economic aspects and competition between different breeding companies, have led to an enormous narrowing of the genetic basis of commercial
maize hybrids. Concurrently, there is usually a small number of often related hybrids of similar
technological and nutritional quality present on the market. Six best-selling ZP maize hybrids
in the previous ten years were chosen as the subject of this study. In the period from 2009-2018,
both the yield components and parameters of technological and nutritional quality of the grain
were studied. It was found that the examined parameters varied a lot in different years of production and that some parameters were more influenced by genotype (number of kernel rows per ear,
portion of pericarp, and crude fiber content) while others were more influenced by the environment (kernel weight per ear, total protein, starch, and oil content, respectively). The amount and
the distribution of precipitation during the vegetative period affected not only the yield components i.e. grain yield but also the technological qualitz parametres and nutritional properties of
the grain. Based on the observed parental components, it is evident that they are related hybrids
that, despite the high genotype by environment interactions, consequently have similar parameters of technological quality and nutritional composition. The imperative is to develop special
breeding programs aiming to create both the starting material and parental components to provide hybrids of improved technological quality and nutritional properties on the market. In that
way, the needs of the processing industry for raw materials of certain characteristics can be met., U procesu hibridnog oplemenjivanja kukuruza, najviše pažnje se posvećuje povećanju nivoa
i stabilnosti prinosa, dok je kvalitet zrna od sekundarnog značaja. Sami principi hibridnog oplemenjivanja, kao i ekonomski aspekti i konkurencija između različitih oplemenjivačkih kuća,
doveli su do enormnog suženja genetičke osnove komercijalnih hibrida kukuruza. Na tržištu je
istovremeno najčešće prisutan mali broj, često srodnih hibrida sličnog tehnološkog i nutritivnog
kvaliteta. Za ovo istraživanje je odabrano šest ZP hibrida kukuruza najprodavanijih u prethodnih
deset godina. U periodu od 2009-2018. godine, praćene su, kako komponente prinosa, tako i
parametri tehnološkog i nutritivnog kvaliteta zrna. Utvrđeno je da su ispitivani parametri veoma
varirali u različitim godinama gajenja, kao i da su neki parametri bili pod većim uticajem genotipa (broj redova zrna, udeo perikarpa i sirove celuloze), a neki pod većim uticajem spoljašnje sredine (masa zrna po klipu, udeo ukupnih proteina, skroba i ulja, respektivno). Količina i raspored
padavina u toku vegetativnog perioda su uticali ne samo na komponente prinosa, odnosno na
prinos zrna, već i na parametre tehnološkog kvaliteta i nutritivna svojstva. Na osnovu posmatranih roditeljskih komponenata, evidentno je da se radi o srodnim hibridima koji, bez obzira na
visoke interakcije između genotipa i spoljašnje sredine, posledično imaju slične parametre tehnološkog kvaliteta kao i nutritivni sastav. Neophodno je razvijati posebne oplemenjivačke programe
koji imaju za cilj stvaranje kako početnog materijala, tako i roditeljskih komponenata, kako bi
se na tržištu obezbedili hibridi poboljšanog tehnološkog kvaliteta i nutritivnih svojstava i na taj
način zadovoljile potrebe prerađivačke industrije za sirovinama određenih karakteristika.",
publisher = "Belgrade : Serbian Association of Plant Breedings and Seed Producers, Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research, Varijabilnost i međuzavisnost osnovnih parametara tehnološkog kvaliteta hibrida kukuruza u dugoročnim istraživanjima",
volume = "27",
number = "2",
pages = "21-33",
doi = "10.5937/SelSem2102021N"
}
Nikolić, V., Babić, V., Kravić, N., Filipović, M., Žilić, S., Simić, M.,& Radosavljević, M.. (2022-01-21). The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research. in Selekcija i semenarstvo
Belgrade : Serbian Association of Plant Breedings and Seed Producers., 27(2), 21-33.
https://doi.org/10.5937/SelSem2102021N
Nikolić V, Babić V, Kravić N, Filipović M, Žilić S, Simić M, Radosavljević M. The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research. in Selekcija i semenarstvo. 2022;27(2):21-33.
doi:10.5937/SelSem2102021N .
Nikolić, Valentina, Babić, Vojka, Kravić, Natalija, Filipović, Milomir, Žilić, Slađana, Simić, Marijana, Radosavljević, Milica, "The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research" in Selekcija i semenarstvo, 27, no. 2 (2022-01-21):21-33,
https://doi.org/10.5937/SelSem2102021N . .

Properties of maize hybrid grain for utilization in wet milling

Nikolić, Valentina; Milašinović Šeremešić, Marija; Radosavljević, Milica; Simić, Marijana; Žilić, Slađana

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2022)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Milašinović Šeremešić, Marija
AU  - Radosavljević, Milica
AU  - Simić, Marijana
AU  - Žilić, Slađana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/988
AB  - A grain of eight selected hybrids from the Maize Research Institute Zemun Polje was used as
the subject of this study. The kernel structure, physical properties and chemical composition of
the grain and starch (amylose content) were analyzed before the laboratory wet milling process for
starch extraction. The in vitro enzymatic digestibility of the isolated starch was also determined.
The wet milling properties were later correlated to the physicochemical characteristics of the
grain. The results showed that hybrid ZP 677 had the highest starch yield, followed by hybrid ZP
704wx, while the highest starch recovery was observed for hybrid ZP 341. The highest bran yield
(10.52%) was obtained by hybrid ZP 434, and the highest germ yield (8.86%) by hybrid ZP 172/8.
The correlation analysis showed that starch yield was positively correlated to 1000-kernel weight
and crude fiber content, while oil content negatively influenced starch extraction and yield. The
starch digestibility was negatively correlated to the amylose content. These findings can be useful
for the starch processing industry as well as in the production of ethanol and alcoholic drinks.
AB  - Kao predmet ovog istraživanja korišćeno je zrno osam odabranih hibrida Instituta za kukuruz
„Zemun Polje”. Struktura zrna, fizička svojstva i hemijski sastav zrna i skroba (sadržaj amiloze)
analizirani su primenom laboratorijskog procesa mokrog mlevenja za izolovanje skroba. Takođe
je određena in vitro enzimska svarljivost izolovanog skroba. Svojstva mokrog mlevenja su potom
korelisana sa fizičko-hemijskim karakteristikama zrna. Rezultati su pokazali da je najviši prinos
skroba imao hibrid ZP 677, a zatim hibrid ZP 704wx, dok je najviša iskoristljivost skroba uočena
kod hibrida ZP 341. Najviši prinos mekinja (10,52%) dobijen je kod hibrida ZP 434, a najviši prinos klice (8,86%) utvrđen je kod hibrida ZP 172/8. Korelaciona analiza je pokazala da je prinos
skroba bio u pozitivnoj vezi sa apsolutnom masom zrna i sadržajem sirovih vlakana, dok je sadržaj ulja negativno uticao na izolaciju skroba i njegov prinos. Svarljivost skroba je bila u negativnoj
korelaciji sa sadržajem amiloze. Ova saznanja mogu biti korisna za industriju prerade skroba, kao
i za proizvodnju etanola i alkoholnih pića.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
PB  - Serbian Association of Plant Breedings and Seed Producers, Belgrade
T2  - Selekcija i semenarstvo
T1  - Properties of maize hybrid grain for utilization in wet milling
T1  - Svojstva zrna hibrida kukuruza za primenu u mokrom mlevenju
VL  - 28
IS  - 1
SP  - 67
EP  - 76
DO  - 10.5937/SelSem2201067N
ER  - 
@article{
author = "Nikolić, Valentina and Milašinović Šeremešić, Marija and Radosavljević, Milica and Simić, Marijana and Žilić, Slađana",
year = "2022",
abstract = "A grain of eight selected hybrids from the Maize Research Institute Zemun Polje was used as
the subject of this study. The kernel structure, physical properties and chemical composition of
the grain and starch (amylose content) were analyzed before the laboratory wet milling process for
starch extraction. The in vitro enzymatic digestibility of the isolated starch was also determined.
The wet milling properties were later correlated to the physicochemical characteristics of the
grain. The results showed that hybrid ZP 677 had the highest starch yield, followed by hybrid ZP
704wx, while the highest starch recovery was observed for hybrid ZP 341. The highest bran yield
(10.52%) was obtained by hybrid ZP 434, and the highest germ yield (8.86%) by hybrid ZP 172/8.
The correlation analysis showed that starch yield was positively correlated to 1000-kernel weight
and crude fiber content, while oil content negatively influenced starch extraction and yield. The
starch digestibility was negatively correlated to the amylose content. These findings can be useful
for the starch processing industry as well as in the production of ethanol and alcoholic drinks., Kao predmet ovog istraživanja korišćeno je zrno osam odabranih hibrida Instituta za kukuruz
„Zemun Polje”. Struktura zrna, fizička svojstva i hemijski sastav zrna i skroba (sadržaj amiloze)
analizirani su primenom laboratorijskog procesa mokrog mlevenja za izolovanje skroba. Takođe
je određena in vitro enzimska svarljivost izolovanog skroba. Svojstva mokrog mlevenja su potom
korelisana sa fizičko-hemijskim karakteristikama zrna. Rezultati su pokazali da je najviši prinos
skroba imao hibrid ZP 677, a zatim hibrid ZP 704wx, dok je najviša iskoristljivost skroba uočena
kod hibrida ZP 341. Najviši prinos mekinja (10,52%) dobijen je kod hibrida ZP 434, a najviši prinos klice (8,86%) utvrđen je kod hibrida ZP 172/8. Korelaciona analiza je pokazala da je prinos
skroba bio u pozitivnoj vezi sa apsolutnom masom zrna i sadržajem sirovih vlakana, dok je sadržaj ulja negativno uticao na izolaciju skroba i njegov prinos. Svarljivost skroba je bila u negativnoj
korelaciji sa sadržajem amiloze. Ova saznanja mogu biti korisna za industriju prerade skroba, kao
i za proizvodnju etanola i alkoholnih pića.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd, Serbian Association of Plant Breedings and Seed Producers, Belgrade",
journal = "Selekcija i semenarstvo",
title = "Properties of maize hybrid grain for utilization in wet milling, Svojstva zrna hibrida kukuruza za primenu u mokrom mlevenju",
volume = "28",
number = "1",
pages = "67-76",
doi = "10.5937/SelSem2201067N"
}
Nikolić, V., Milašinović Šeremešić, M., Radosavljević, M., Simić, M.,& Žilić, S.. (2022). Properties of maize hybrid grain for utilization in wet milling. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 28(1), 67-76.
https://doi.org/10.5937/SelSem2201067N
Nikolić V, Milašinović Šeremešić M, Radosavljević M, Simić M, Žilić S. Properties of maize hybrid grain for utilization in wet milling. in Selekcija i semenarstvo. 2022;28(1):67-76.
doi:10.5937/SelSem2201067N .
Nikolić, Valentina, Milašinović Šeremešić, Marija, Radosavljević, Milica, Simić, Marijana, Žilić, Slađana, "Properties of maize hybrid grain for utilization in wet milling" in Selekcija i semenarstvo, 28, no. 1 (2022):67-76,
https://doi.org/10.5937/SelSem2201067N . .

Status of essential elements in soil and grain of organically produced maize, spelt, and soybean

Dragičević, Vesna; Stoiljkovic, Milovan; Brankov, Milan; Tolimir, Miodrag; Tabaković, Marijenka; Dodevska, Margarita; Simic, Milena

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2022)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Stoiljkovic, Milovan
AU  - Brankov, Milan
AU  - Tolimir, Miodrag
AU  - Tabaković, Marijenka
AU  - Dodevska, Margarita
AU  - Simic, Milena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/981
AB  - Organic agriculture offers many benefits through the increased nutritional quality of produced crops, agro-ecosystem preservation, and climate change mitigation. The development of an efficient nutrient management strategy in low-input systems, such as organic agriculture, which supports soil fertility and essential nutrients absorption by crops, is continually exploring. Thus, a study with maize–spelt–soybean rotation during a 5-year period in organic production was established to evaluate the variability in soil organic matter (SOM) and the status of available elements: N, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si from the soil, as well as grain yield (GY) and the content of protein, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si concentration in the grain of spelt, maize, and soybean. Significant variations in mineral elements in the soil, GY, and grain composition were detected. Spelt achieved the highest average GY, while soybean grain was the richest in a majority of examined nutrients. The soil Ca content was important for GY, while the protein level in grain was generally tied to the Mn level in the soil. It was recognized that soil–crop crosstalk is an important strategy for macro- and micro-nutrients management in the soil and grain of organically produced spelt, maize, and soybean. While a reduction in the GY and protein concentration in grain was present over time, it was established that a low-input system under dry-farming conditions supports nutrient availability and accumulation in grain, under semi-arid agro-ecological conditions of central Serbia.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Agriculture
T1  - Status of essential elements in soil and grain of organically produced maize, spelt, and soybean
VL  - 12
IS  - 5
SP  - 702
DO  - https://doi.org/10.3390/agriculture12050702
ER  - 
@article{
author = "Dragičević, Vesna and Stoiljkovic, Milovan and Brankov, Milan and Tolimir, Miodrag and Tabaković, Marijenka and Dodevska, Margarita and Simic, Milena",
year = "2022",
abstract = "Organic agriculture offers many benefits through the increased nutritional quality of produced crops, agro-ecosystem preservation, and climate change mitigation. The development of an efficient nutrient management strategy in low-input systems, such as organic agriculture, which supports soil fertility and essential nutrients absorption by crops, is continually exploring. Thus, a study with maize–spelt–soybean rotation during a 5-year period in organic production was established to evaluate the variability in soil organic matter (SOM) and the status of available elements: N, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si from the soil, as well as grain yield (GY) and the content of protein, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si concentration in the grain of spelt, maize, and soybean. Significant variations in mineral elements in the soil, GY, and grain composition were detected. Spelt achieved the highest average GY, while soybean grain was the richest in a majority of examined nutrients. The soil Ca content was important for GY, while the protein level in grain was generally tied to the Mn level in the soil. It was recognized that soil–crop crosstalk is an important strategy for macro- and micro-nutrients management in the soil and grain of organically produced spelt, maize, and soybean. While a reduction in the GY and protein concentration in grain was present over time, it was established that a low-input system under dry-farming conditions supports nutrient availability and accumulation in grain, under semi-arid agro-ecological conditions of central Serbia.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Agriculture",
title = "Status of essential elements in soil and grain of organically produced maize, spelt, and soybean",
volume = "12",
number = "5",
pages = "702",
doi = "https://doi.org/10.3390/agriculture12050702"
}
Dragičević, V., Stoiljkovic, M., Brankov, M., Tolimir, M., Tabaković, M., Dodevska, M.,& Simic, M.. (2022). Status of essential elements in soil and grain of organically produced maize, spelt, and soybean. in Agriculture
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 12(5), 702.
https://doi.org/https://doi.org/10.3390/agriculture12050702
Dragičević V, Stoiljkovic M, Brankov M, Tolimir M, Tabaković M, Dodevska M, Simic M. Status of essential elements in soil and grain of organically produced maize, spelt, and soybean. in Agriculture. 2022;12(5):702.
doi:https://doi.org/10.3390/agriculture12050702 .
Dragičević, Vesna, Stoiljkovic, Milovan, Brankov, Milan, Tolimir, Miodrag, Tabaković, Marijenka, Dodevska, Margarita, Simic, Milena, "Status of essential elements in soil and grain of organically produced maize, spelt, and soybean" in Agriculture, 12, no. 5 (2022):702,
https://doi.org/https://doi.org/10.3390/agriculture12050702 . .

Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study

Bartolić, Dragana; Mutavdzic, Dragosav; Carstensen, Jens Michael; Stanković, Slavica; Nikolić, Milica; Krstović, Saša; Radotić, Ksenija

(Berlin : Springer Nature, 2022)

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mutavdzic, Dragosav
AU  - Carstensen, Jens Michael
AU  - Stanković, Slavica
AU  - Nikolić, Milica
AU  - Krstović, Saša
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/838
AB  - Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non‑invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B 1 (AFB 1 ) from the uncontaminated seeds. The AFB 1‑contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB 1‑contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB 1‑contaminated seeds showed a 7.9 to 9.6‑fold increase in the seed
reflectance in the VIS region, and 10.4 and 12.2‑fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non‑invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.
PB  - Berlin : Springer Nature
T2  - Scientific Reports
T1  - Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study
VL  - 12
SP  - 4849
DO  - 10.1038/s41598-022-08352-4
ER  - 
@article{
author = "Bartolić, Dragana and Mutavdzic, Dragosav and Carstensen, Jens Michael and Stanković, Slavica and Nikolić, Milica and Krstović, Saša and Radotić, Ksenija",
year = "2022",
abstract = "Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non‑invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B 1 (AFB 1 ) from the uncontaminated seeds. The AFB 1‑contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB 1‑contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB 1‑contaminated seeds showed a 7.9 to 9.6‑fold increase in the seed
reflectance in the VIS region, and 10.4 and 12.2‑fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non‑invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.",
publisher = "Berlin : Springer Nature",
journal = "Scientific Reports",
title = "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study",
volume = "12",
pages = "4849",
doi = "10.1038/s41598-022-08352-4"
}
Bartolić, D., Mutavdzic, D., Carstensen, J. M., Stanković, S., Nikolić, M., Krstović, S.,& Radotić, K.. (2022). Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports
Berlin : Springer Nature., 12, 4849.
https://doi.org/10.1038/s41598-022-08352-4
Bartolić D, Mutavdzic D, Carstensen JM, Stanković S, Nikolić M, Krstović S, Radotić K. Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports. 2022;12:4849.
doi:10.1038/s41598-022-08352-4 .
Bartolić, Dragana, Mutavdzic, Dragosav, Carstensen, Jens Michael, Stanković, Slavica, Nikolić, Milica, Krstović, Saša, Radotić, Ksenija, "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study" in Scientific Reports, 12 (2022):4849,
https://doi.org/10.1038/s41598-022-08352-4 . .
1

Suitability of the selected local maize hybrids for silage production

Nikolić, Valentina; Simić, Marijana; Žilić, Slađana; Kravić, Natalija; Babić, Vojka; Filipović, Milomir; Srdić, Jelena

(Cacak : University of Kragujevac, Faculty of Agronomy, 2022)

TY  - CONF
AU  - Nikolić, Valentina
AU  - Simić, Marijana
AU  - Žilić, Slađana
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Filipović, Milomir
AU  - Srdić, Jelena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/874
AB  - The main goal of this study was to observe the properties of fifteen
different genotypes of maize hybrids from Serbia in order to determine their
suitability for the production of high-quality silage for ruminant feed. The research
was conducted in a two-year field experiment at the location of the Maize Research
Institute in Zemun Polje, Serbia, and the laboratory analyses included yield structure
of the investigated maize hybrids, assessment of the lignocellulosic fiber
composition, as well as the in vitro dry matter digestibility of the whole plant
samples. All maize hybrids have shown good quality traits that are a prerequisite for
the production of high-quality silage.
PB  - Cacak : University of Kragujevac, Faculty of Agronomy
C3  - 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova
T1  - Suitability of the selected local maize hybrids for silage production
SP  - 105
EP  - 110
DO  - 10.46793/SBT27.105N
ER  - 
@conference{
author = "Nikolić, Valentina and Simić, Marijana and Žilić, Slađana and Kravić, Natalija and Babić, Vojka and Filipović, Milomir and Srdić, Jelena",
year = "2022",
abstract = "The main goal of this study was to observe the properties of fifteen
different genotypes of maize hybrids from Serbia in order to determine their
suitability for the production of high-quality silage for ruminant feed. The research
was conducted in a two-year field experiment at the location of the Maize Research
Institute in Zemun Polje, Serbia, and the laboratory analyses included yield structure
of the investigated maize hybrids, assessment of the lignocellulosic fiber
composition, as well as the in vitro dry matter digestibility of the whole plant
samples. All maize hybrids have shown good quality traits that are a prerequisite for
the production of high-quality silage.",
publisher = "Cacak : University of Kragujevac, Faculty of Agronomy",
journal = "27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova",
title = "Suitability of the selected local maize hybrids for silage production",
pages = "105-110",
doi = "10.46793/SBT27.105N"
}
Nikolić, V., Simić, M., Žilić, S., Kravić, N., Babić, V., Filipović, M.,& Srdić, J.. (2022). Suitability of the selected local maize hybrids for silage production. in 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova
Cacak : University of Kragujevac, Faculty of Agronomy., 105-110.
https://doi.org/10.46793/SBT27.105N
Nikolić V, Simić M, Žilić S, Kravić N, Babić V, Filipović M, Srdić J. Suitability of the selected local maize hybrids for silage production. in 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova. 2022;:105-110.
doi:10.46793/SBT27.105N .
Nikolić, Valentina, Simić, Marijana, Žilić, Slađana, Kravić, Natalija, Babić, Vojka, Filipović, Milomir, Srdić, Jelena, "Suitability of the selected local maize hybrids for silage production" in 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova (2022):105-110,
https://doi.org/10.46793/SBT27.105N . .

Nutritive quality and plant digestibility of silage maize hybrids from Serbia

Nikolić, Valentina; Simić, Marijana; Žilić, Slađana

(Kiev : Ukrainian Institute for Plant Variety Examination, 2022)

TY  - CONF
AU  - Nikolić, Valentina
AU  - Simić, Marijana
AU  - Žilić, Slađana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/898
AB  - The quality of five silage maize hybrids grown
in 2020 at five different locations in Serbia (two in
the northern province of Vojvodina (Srem) and three
in Central Serbia) was tested in 2021 in the laboratory
of the Department of Food Technology and
Biochemistry of the Maize Research Institute “Zemun
Polje“. The dry matter content, lignocellulose
fiber content, and in vitro dry matter digestibility
(IVDMD) of of the whole plant maize hybrids: ZP
707, ZP 7357, ZP 790, ZP 8701, and ZP 873 were
investigated. The 7001 hybrid was used as a standard.
The average dry matter content varied between
37.77±1.88% (ZP 790) and 42.64±7.19% (ZP
707), while in vitro dry matter digestibility ranged
from 58.77±1.96% (ZP 8701) to 63.77±2.09% (ZP
707). The share, as well as the type of the plant
cell wall components – lignocellulosic fibers, determines
the nutritional quality of silage maize
as animal feed. The NDF content ranged from As
the NDF content increases with maize maturity,
animals show a tendency for lower silage intake.
However, although in some cases NDF can be considered
a negative indicator of silage quality, NDF
is a necessary component of ruminant nutrition.
Based on the achieved results, it can be concluded
that hybrids ZP 707 and ZP 7357 proved to be the
best silage maize forms. At most locations, these
hybrids achieved better results than the standard.
The ZP 707 hybrid on average had the highest dry
matter content, the highest digestibility of dry
matter, as well as the lowest content of all lignocellulosic
fibers, which all indicate its potential
as silage maize form. The lack of hybrids ZP 707
and ZP 7357 can be considered the fact that the
dry matter content of the whole plant showed to be
less stable in different agro-ecological conditions.In terms of digestibility and dry matter content,
the ZP 790 hybrid can be singled out as the most
stable. The ZP 790 hybrid is less recommended for
Srem, but in Central Serbia, it could match the ZP
707 and ZP 7357 hybrids. ZP 8701 proved to be the
weakest hybrid at all locations, followed by ZP 873.
The in vitro digestibility and dry matter content
of the whole plant of the investigated hybrids were
far more stable in Srem than in Central Serbia.
These findings can be of great importance for future
breeding programs directed toward creating
new and improved silage maize hybrids.
PB  - Kiev : Ukrainian Institute for Plant Variety Examination
C3  - 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings
T1  - Nutritive quality and plant digestibility of silage maize hybrids from Serbia
SP  - 78
EP  - 79
ER  - 
@conference{
author = "Nikolić, Valentina and Simić, Marijana and Žilić, Slađana",
year = "2022",
abstract = "The quality of five silage maize hybrids grown
in 2020 at five different locations in Serbia (two in
the northern province of Vojvodina (Srem) and three
in Central Serbia) was tested in 2021 in the laboratory
of the Department of Food Technology and
Biochemistry of the Maize Research Institute “Zemun
Polje“. The dry matter content, lignocellulose
fiber content, and in vitro dry matter digestibility
(IVDMD) of of the whole plant maize hybrids: ZP
707, ZP 7357, ZP 790, ZP 8701, and ZP 873 were
investigated. The 7001 hybrid was used as a standard.
The average dry matter content varied between
37.77±1.88% (ZP 790) and 42.64±7.19% (ZP
707), while in vitro dry matter digestibility ranged
from 58.77±1.96% (ZP 8701) to 63.77±2.09% (ZP
707). The share, as well as the type of the plant
cell wall components – lignocellulosic fibers, determines
the nutritional quality of silage maize
as animal feed. The NDF content ranged from As
the NDF content increases with maize maturity,
animals show a tendency for lower silage intake.
However, although in some cases NDF can be considered
a negative indicator of silage quality, NDF
is a necessary component of ruminant nutrition.
Based on the achieved results, it can be concluded
that hybrids ZP 707 and ZP 7357 proved to be the
best silage maize forms. At most locations, these
hybrids achieved better results than the standard.
The ZP 707 hybrid on average had the highest dry
matter content, the highest digestibility of dry
matter, as well as the lowest content of all lignocellulosic
fibers, which all indicate its potential
as silage maize form. The lack of hybrids ZP 707
and ZP 7357 can be considered the fact that the
dry matter content of the whole plant showed to be
less stable in different agro-ecological conditions.In terms of digestibility and dry matter content,
the ZP 790 hybrid can be singled out as the most
stable. The ZP 790 hybrid is less recommended for
Srem, but in Central Serbia, it could match the ZP
707 and ZP 7357 hybrids. ZP 8701 proved to be the
weakest hybrid at all locations, followed by ZP 873.
The in vitro digestibility and dry matter content
of the whole plant of the investigated hybrids were
far more stable in Srem than in Central Serbia.
These findings can be of great importance for future
breeding programs directed toward creating
new and improved silage maize hybrids.",
publisher = "Kiev : Ukrainian Institute for Plant Variety Examination",
journal = "10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings",
title = "Nutritive quality and plant digestibility of silage maize hybrids from Serbia",
pages = "78-79"
}
Nikolić, V., Simić, M.,& Žilić, S.. (2022). Nutritive quality and plant digestibility of silage maize hybrids from Serbia. in 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings
Kiev : Ukrainian Institute for Plant Variety Examination., 78-79.
Nikolić V, Simić M, Žilić S. Nutritive quality and plant digestibility of silage maize hybrids from Serbia. in 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings. 2022;:78-79..
Nikolić, Valentina, Simić, Marijana, Žilić, Slađana, "Nutritive quality and plant digestibility of silage maize hybrids from Serbia" in 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings (2022):78-79.

Acrylamide in Corn-Based Thermally Processed Foods: A Review

Žilić, Slađana; Nikolić, Valentina; Mogol, Burce Atac; Hamzalioglu, Aytul; Tas, Neslihan Goncuoglu; Kocadagli, Tolgahan; Simić, Marijana; Gokmen, Vural

(Washington : ACS Publications, 2022)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Nikolić, Valentina
AU  - Mogol, Burce Atac
AU  - Hamzalioglu, Aytul
AU  - Tas, Neslihan Goncuoglu
AU  - Kocadagli, Tolgahan
AU  - Simić, Marijana
AU  - Gokmen, Vural
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/855
AB  - Widely consumed thermally processed corn-based foods can have a great contribution to acrylamide dietary intake,
thus bearing a high public health risk and requiring attention and application of strategies for its reduction. This paper reviews the
literature on the acrylamide content of corn-based food products present in the market around the world. The potential of corn for
acrylamide formation due to its content of free asparagine and reducing sugars is described. Human exposure to acrylamide from
corn-based foods is also discussed. The content of acrylamide in corn/tortilla chips, popcorn, and corn flakes, as widely consumed
products all over the world, is reported in the literature to be between 5 and 6360 μg/kg, between <LOD and 2220 μg/kg and
between <LOD and 1186 μg/kg, respectively. Although these products are important acrylamide sources in the common diet of all
age populations, higher intake values occurred among younger generations.
PB  - Washington : ACS Publications
T2  - Journal of agricultural and food chemistry
T1  - Acrylamide in Corn-Based Thermally Processed Foods: A Review
VL  - 70
SP  - 4165
EP  - 4181
DO  - 10.1021/acs.jafc.1c07249
ER  - 
@article{
author = "Žilić, Slađana and Nikolić, Valentina and Mogol, Burce Atac and Hamzalioglu, Aytul and Tas, Neslihan Goncuoglu and Kocadagli, Tolgahan and Simić, Marijana and Gokmen, Vural",
year = "2022",
abstract = "Widely consumed thermally processed corn-based foods can have a great contribution to acrylamide dietary intake,
thus bearing a high public health risk and requiring attention and application of strategies for its reduction. This paper reviews the
literature on the acrylamide content of corn-based food products present in the market around the world. The potential of corn for
acrylamide formation due to its content of free asparagine and reducing sugars is described. Human exposure to acrylamide from
corn-based foods is also discussed. The content of acrylamide in corn/tortilla chips, popcorn, and corn flakes, as widely consumed
products all over the world, is reported in the literature to be between 5 and 6360 μg/kg, between <LOD and 2220 μg/kg and
between <LOD and 1186 μg/kg, respectively. Although these products are important acrylamide sources in the common diet of all
age populations, higher intake values occurred among younger generations.",
publisher = "Washington : ACS Publications",
journal = "Journal of agricultural and food chemistry",
title = "Acrylamide in Corn-Based Thermally Processed Foods: A Review",
volume = "70",
pages = "4165-4181",
doi = "10.1021/acs.jafc.1c07249"
}
Žilić, S., Nikolić, V., Mogol, B. A., Hamzalioglu, A., Tas, N. G., Kocadagli, T., Simić, M.,& Gokmen, V.. (2022). Acrylamide in Corn-Based Thermally Processed Foods: A Review. in Journal of agricultural and food chemistry
Washington : ACS Publications., 70, 4165-4181.
https://doi.org/10.1021/acs.jafc.1c07249
Žilić S, Nikolić V, Mogol BA, Hamzalioglu A, Tas NG, Kocadagli T, Simić M, Gokmen V. Acrylamide in Corn-Based Thermally Processed Foods: A Review. in Journal of agricultural and food chemistry. 2022;70:4165-4181.
doi:10.1021/acs.jafc.1c07249 .
Žilić, Slađana, Nikolić, Valentina, Mogol, Burce Atac, Hamzalioglu, Aytul, Tas, Neslihan Goncuoglu, Kocadagli, Tolgahan, Simić, Marijana, Gokmen, Vural, "Acrylamide in Corn-Based Thermally Processed Foods: A Review" in Journal of agricultural and food chemistry, 70 (2022):4165-4181,
https://doi.org/10.1021/acs.jafc.1c07249 . .
2

Possibilities of dried distillers' grains with solubles application in animal feed

Nikolić, Valentina; Milašinović Šeremešić, Marija; Radosavljević, Milica; Simić, Marijana; Žilić, Slađana; Vančetović, Jelena; Božinović, Sofija

(Novi Sad : National Society of Processing and Energy in Agriculture, 2021-11-30)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Milašinović Šeremešić, Marija
AU  - Radosavljević, Milica
AU  - Simić, Marijana
AU  - Žilić, Slađana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
PY  - 2021-11-30
UR  - http://rik.mrizp.rs/handle/123456789/845
AB  - The possibility of utilization of dried distillers’ grains with solubles (DDGS) in animal feed was investigated. Samples of maize
hybrids ZP 548 and ZP 655b, and DDGS obtained from hybrids ZP 548 and ZP 548c were used as components of the feed. The total
protein content ranged from 12.42% to 31.18%, moisture from 5.49% to 9.55, and ash content from 1.85% to 4.37%. The contents of
NDF, ADF, ADL, hemicellulose and cellulose fibers ranged from: 13.90% to 48.13%; 2.9% to 20.69%; 0.27% to 2.44%; 10.69% to
30.17%, 2.62% to 18.32%, respectively. In vitro, dry matter digestibility ranged from 55.20% to 89.76%. It was concluded that the
samples of DDG obtained from red and yellow maize hybrids kernel are very suitable as components for the preparation of animal
feed.
AB  - Ispitivana je mogućnost primene suve kukuruzne džibre u smešama za ishranu domaćih životinja. Kao komponente korišćeni su
uzorci cele biljke i zrna hibrida ZP 548 i ZP 655b i uzorci džibre dobijeni od hibrida ZP 548 i ZP 548c (hibrid crvenog zrna). Uzorci
kukuruzne džibre, sporednog produkta iz proizvodnje bioetanola, dobijeni su postupkom odvojene dvostepene hidrolize i fermentacije
skroba iz kukuruznog brašna. Metoda se zasniva na korišćenju enzimskih preparata u fazama hidrolize i primeni kvasca S. cerevisiae
var. ellipsoideus tokom fermentacije. Uzorci džibre sušeni su u ventilacionoj sušnici na temperaturi 60˚C u trajanju od 48h, a potom
samleveni u laboratorijskom mlinu. Sadržaj proteina kretao se od 12,42% do 31,18%, vlage od 5,49% do 9,55%, a pepela od 1,85%
do 4,37%. Sadržaj NDF-a kretao se od 13,90% (zrno ZP 548 + 15% džibre ZP 548) do 48,13% (cela biljka ZP 655b + 20% džibre
ZP 548c); ADF-a od 2,96% (zrno ZP 548 +15% džibre ZP 548c) do 20,69% (cela biljka ZP 655b + 10% džibre ZP 548c); ADL-a od
0,27% do 2,44f%: hemiceluloze od 10,69% do 30,17% i celuloze od 2,62% do 18,32%. In vitro svarljivost suve materije određena
enzimskom pepsin-celulaznom metodom kretala se u rasponu od 55,20% do 89,76%. Na osnovu dobijenih rezultata ustanovljeno je
da su uzorci suve kukuruzne džibre dobijeni od zrna žutog i crvenog hibrida kukuruza pogodni kao hranivo za pripremu smeša za
ishranu domaćih životinja.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Possibilities of dried distillers' grains with solubles application in animal feed
T1  - Mogućnosti primene suve destilerijske džibre u smešama za ishranu životinja
VL  - 25
IS  - 3
SP  - 96
EP  - 100
DO  - 10.5937/jpea25-34890
ER  - 
@article{
author = "Nikolić, Valentina and Milašinović Šeremešić, Marija and Radosavljević, Milica and Simić, Marijana and Žilić, Slađana and Vančetović, Jelena and Božinović, Sofija",
year = "2021-11-30",
abstract = "The possibility of utilization of dried distillers’ grains with solubles (DDGS) in animal feed was investigated. Samples of maize
hybrids ZP 548 and ZP 655b, and DDGS obtained from hybrids ZP 548 and ZP 548c were used as components of the feed. The total
protein content ranged from 12.42% to 31.18%, moisture from 5.49% to 9.55, and ash content from 1.85% to 4.37%. The contents of
NDF, ADF, ADL, hemicellulose and cellulose fibers ranged from: 13.90% to 48.13%; 2.9% to 20.69%; 0.27% to 2.44%; 10.69% to
30.17%, 2.62% to 18.32%, respectively. In vitro, dry matter digestibility ranged from 55.20% to 89.76%. It was concluded that the
samples of DDG obtained from red and yellow maize hybrids kernel are very suitable as components for the preparation of animal
feed., Ispitivana je mogućnost primene suve kukuruzne džibre u smešama za ishranu domaćih životinja. Kao komponente korišćeni su
uzorci cele biljke i zrna hibrida ZP 548 i ZP 655b i uzorci džibre dobijeni od hibrida ZP 548 i ZP 548c (hibrid crvenog zrna). Uzorci
kukuruzne džibre, sporednog produkta iz proizvodnje bioetanola, dobijeni su postupkom odvojene dvostepene hidrolize i fermentacije
skroba iz kukuruznog brašna. Metoda se zasniva na korišćenju enzimskih preparata u fazama hidrolize i primeni kvasca S. cerevisiae
var. ellipsoideus tokom fermentacije. Uzorci džibre sušeni su u ventilacionoj sušnici na temperaturi 60˚C u trajanju od 48h, a potom
samleveni u laboratorijskom mlinu. Sadržaj proteina kretao se od 12,42% do 31,18%, vlage od 5,49% do 9,55%, a pepela od 1,85%
do 4,37%. Sadržaj NDF-a kretao se od 13,90% (zrno ZP 548 + 15% džibre ZP 548) do 48,13% (cela biljka ZP 655b + 20% džibre
ZP 548c); ADF-a od 2,96% (zrno ZP 548 +15% džibre ZP 548c) do 20,69% (cela biljka ZP 655b + 10% džibre ZP 548c); ADL-a od
0,27% do 2,44f%: hemiceluloze od 10,69% do 30,17% i celuloze od 2,62% do 18,32%. In vitro svarljivost suve materije određena
enzimskom pepsin-celulaznom metodom kretala se u rasponu od 55,20% do 89,76%. Na osnovu dobijenih rezultata ustanovljeno je
da su uzorci suve kukuruzne džibre dobijeni od zrna žutog i crvenog hibrida kukuruza pogodni kao hranivo za pripremu smeša za
ishranu domaćih životinja.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Possibilities of dried distillers' grains with solubles application in animal feed, Mogućnosti primene suve destilerijske džibre u smešama za ishranu životinja",
volume = "25",
number = "3",
pages = "96-100",
doi = "10.5937/jpea25-34890"
}
Nikolić, V., Milašinović Šeremešić, M., Radosavljević, M., Simić, M., Žilić, S., Vančetović, J.,& Božinović, S.. (2021-11-30). Possibilities of dried distillers' grains with solubles application in animal feed. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 25(3), 96-100.
https://doi.org/10.5937/jpea25-34890
Nikolić V, Milašinović Šeremešić M, Radosavljević M, Simić M, Žilić S, Vančetović J, Božinović S. Possibilities of dried distillers' grains with solubles application in animal feed. in Journal on Processing and Energy in Agriculture. 2021;25(3):96-100.
doi:10.5937/jpea25-34890 .
Nikolić, Valentina, Milašinović Šeremešić, Marija, Radosavljević, Milica, Simić, Marijana, Žilić, Slađana, Vančetović, Jelena, Božinović, Sofija, "Possibilities of dried distillers' grains with solubles application in animal feed" in Journal on Processing and Energy in Agriculture, 25, no. 3 (2021-11-30):96-100,
https://doi.org/10.5937/jpea25-34890 . .

Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Vasić, Marko G.; Srdić, Jelena; Delić, Nenad; Delić, Nenad

(Novi Sad : National Society of Processing and Energy in Agriculture, 2021-05-07)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Vasić, Marko G.
AU  - Srdić, Jelena
AU  - Delić, Nenad
AU  - Delić, Nenad
PY  - 2021-05-07
UR  - http://rik.mrizp.rs/handle/123456789/846
AB  - Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane
T1  - Possibilities of maize hybrids utilization in canned baby corn production
VL  - 25
IS  - 1
SP  - 16
EP  - 19
DO  - 10.5937/jpea25-30887
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Vasić, Marko G. and Srdić, Jelena and Delić, Nenad and Delić, Nenad",
year = "2021-05-07",
abstract = "Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane, Possibilities of maize hybrids utilization in canned baby corn production",
volume = "25",
number = "1",
pages = "16-19",
doi = "10.5937/jpea25-30887"
}
Nikolić, V., Žilić, S., Simić, M., Vasić, M. G., Srdić, J., Delić, N.,& Delić, N.. (2021-05-07). Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 25(1), 16-19.
https://doi.org/10.5937/jpea25-30887
Nikolić V, Žilić S, Simić M, Vasić MG, Srdić J, Delić N, Delić N. Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture. 2021;25(1):16-19.
doi:10.5937/jpea25-30887 .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Vasić, Marko G., Srdić, Jelena, Delić, Nenad, Delić, Nenad, "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021-05-07):16-19,
https://doi.org/10.5937/jpea25-30887 . .
1

Investigations on the formation of Maillard reaction products in sweet cookies made of different cereals

Žilić, Slađana; Aktaĝ, Işil; Dodig, Dejan; Gökmen, Vural

(Elsevier, 2021-04)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Aktaĝ, Işil
AU  - Dodig, Dejan
AU  - Gökmen, Vural
PY  - 2021-04
UR  - http://rik.mrizp.rs/handle/123456789/893
AB  - In this study, the content of Maillard reaction products from its initial, intermediate and final stage (5-
hydroxymethylfurfural, α-dicarbonyl compounds, furosine, N-ε-carboxymethyllysine and N-ε-carboxyethyllysine)
was measured in sweet cookies made of wholegrain flour of eight genotypes of small-grain cereals
(bread wheat, durum wheat, soft wheat, hard wheat, triticale, rye, hulless barley and hulless oat) and four corn
genotypes (white-, yellow- and red-colored standard seeded corn and blue-colored popping corn). Furthermore, 
the effect of the initial content of sugars, total proteins, free and total lysine in flour on the formation of Maillard
reaction products was investigated using the principle component analysis. 3-deoxyglucosone was the predominant
α-dicarbonyl compound in all cereal cookies and the highest content was measured in those made from
flour of different colored corn genotypes (on average, 98.35, 151.28 and 172.85 mg/kg after baking for 7, 10 and
13 min, respectively). Heating dough at 180 ◦C for 7, 10 and 13 min differently affected the content of 5-hydroxymethylfurfural
and α-dicarbonyl compounds in the cereal cookies. The 5-hydroxymethylfurfural content
gradually increased, while a reduction in 3-deoxyglucosone content was observed in the cookies baked for 13
min except for those made from soft wheat, hulless oat, red- and blue-colored corn. After 7 min of heating, the
content of furosine measured in the cereal cookies reached its maximum (from 320.9 mg/kg in yellow-colored
corn-based cookies to 585.7 mg/kg in hulless oat-based cookies), while N-ε-carboxymethyllysine and N-ε-carboxyethyllysine
showed the opposite trend. The highest content of advanced glycation end products was detected
in cookies also made from hulless oat flour rich in proteins (16.80%) and total lysine (10670.3 mg/kg). The
interrelationship analysis showed that the initial content of sugars in flour of cereals affected 5-hydroxymethylfurfural
and 3-deoxyglucosone formation in the cookies. In addition, a high correlation between protein-bound
Maillard reaction products in the cookies and the total proteins and the total lysine content in the flours was
found.
PB  - Elsevier
T2  - Food Research International
T1  - Investigations on the formation of Maillard reaction products in sweet cookies made of different cereals
VL  - 144
SP  - 110352
DO  - 10.1016/j.foodres.2021.110352
ER  - 
@article{
author = "Žilić, Slađana and Aktaĝ, Işil and Dodig, Dejan and Gökmen, Vural",
year = "2021-04",
abstract = "In this study, the content of Maillard reaction products from its initial, intermediate and final stage (5-
hydroxymethylfurfural, α-dicarbonyl compounds, furosine, N-ε-carboxymethyllysine and N-ε-carboxyethyllysine)
was measured in sweet cookies made of wholegrain flour of eight genotypes of small-grain cereals
(bread wheat, durum wheat, soft wheat, hard wheat, triticale, rye, hulless barley and hulless oat) and four corn
genotypes (white-, yellow- and red-colored standard seeded corn and blue-colored popping corn). Furthermore, 
the effect of the initial content of sugars, total proteins, free and total lysine in flour on the formation of Maillard
reaction products was investigated using the principle component analysis. 3-deoxyglucosone was the predominant
α-dicarbonyl compound in all cereal cookies and the highest content was measured in those made from
flour of different colored corn genotypes (on average, 98.35, 151.28 and 172.85 mg/kg after baking for 7, 10 and
13 min, respectively). Heating dough at 180 ◦C for 7, 10 and 13 min differently affected the content of 5-hydroxymethylfurfural
and α-dicarbonyl compounds in the cereal cookies. The 5-hydroxymethylfurfural content
gradually increased, while a reduction in 3-deoxyglucosone content was observed in the cookies baked for 13
min except for those made from soft wheat, hulless oat, red- and blue-colored corn. After 7 min of heating, the
content of furosine measured in the cereal cookies reached its maximum (from 320.9 mg/kg in yellow-colored
corn-based cookies to 585.7 mg/kg in hulless oat-based cookies), while N-ε-carboxymethyllysine and N-ε-carboxyethyllysine
showed the opposite trend. The highest content of advanced glycation end products was detected
in cookies also made from hulless oat flour rich in proteins (16.80%) and total lysine (10670.3 mg/kg). The
interrelationship analysis showed that the initial content of sugars in flour of cereals affected 5-hydroxymethylfurfural
and 3-deoxyglucosone formation in the cookies. In addition, a high correlation between protein-bound
Maillard reaction products in the cookies and the total proteins and the total lysine content in the flours was
found.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Investigations on the formation of Maillard reaction products in sweet cookies made of different cereals",
volume = "144",
pages = "110352",
doi = "10.1016/j.foodres.2021.110352"
}
Žilić, S., Aktaĝ, I., Dodig, D.,& Gökmen, V.. (2021-04). Investigations on the formation of Maillard reaction products in sweet cookies made of different cereals. in Food Research International
Elsevier., 144, 110352.
https://doi.org/10.1016/j.foodres.2021.110352
Žilić S, Aktaĝ I, Dodig D, Gökmen V. Investigations on the formation of Maillard reaction products in sweet cookies made of different cereals. in Food Research International. 2021;144:110352.
doi:10.1016/j.foodres.2021.110352 .
Žilić, Slađana, Aktaĝ, Işil, Dodig, Dejan, Gökmen, Vural, "Investigations on the formation of Maillard reaction products in sweet cookies made of different cereals" in Food Research International, 144 (2021-04):110352,
https://doi.org/10.1016/j.foodres.2021.110352 . .
5

The role of maize hybrids in current trends of bioethanol production

Nikolić, Valentina; Žilić, Slađana; Radosavljević, Milica; Simić, Marijana

(Belgrade : Serbian Association of Plant Breedings and Seed Producers, 2021-01-19)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Radosavljević, Milica
AU  - Simić, Marijana
PY  - 2021-01-19
UR  - http://rik.mrizp.rs/handle/123456789/852
AB  - Bioethanol is a biofuel that is mostly used as a replacement for fossil fuels worldwide with
yearly production reaching nearly 110 billion liters in 2019. Trends of producing this alternative
fuel are rising and maize is considered as one of the best renewable raw materials for the production of fuel ethanol due to the high content of starch in the grain. Taking into account that
Serbia is one of the most prominent maize producers in Europe, the surpluses of this crop could
be directed toward bioethanol production. Even though there is no organized production and
consumption of bioethanol as an automotive fuel in Serbia, the Serbian Government has recently
introduced some new regulations regarding biofuels. However, due to the reduction of economic
activities since the onset of the COVID-19 pandemic in 2020, the global demand for crude oil has
fallen sharply, negatively affecting the gasoline demand, and thus bioethanol, which makes the
future of this alternative fuel production notably uncertain.
AB  - Bioetanol je biogorivo koje se uglavnom koristi kao zamena za fosilna goriva širom sveta,
a godišnja proizvodnja dostigla je gotovo 110 milijardi litara u 2019. godini. Trendovi proizvodnje ovog alternativnog goriva rastu, a kukuruz se smatra jednom od najboljih obnovljivih
sirovina za proizvodnju bioetanola zahvaljujući visokom sadržaju skroba u zrnu. Uzimajući u
obzir da je Srbija jedan od najistaknutijih proizvođača kukuruza u Evropi, viškovi useva ove
poljoprivredne kulture mogli bi se usmeriti ka proizvodnji bioetanola. Iako u Srbiji ne postoji
organizovana proizvodnja i potrošnja bioetanola kao transportnog goriva, Vlada Republike Srbije nedavno je usvojila nekoliko novih uredbi u vezi sa stavljanjem u promet, obeležavanjem i
potrošnjom biogoriva. Međutim, zbog smanjenja ekonomskih aktivnosti od početka pandemije
virusa COVID-19 tokom 2020. godine, globalna potražnja za sirovom naftom naglo je opala, što
je negativno uticalo na potražnju za benzinom, a time i za bioetanolom, zbog čega je budućnost
proizvodnje ovog alternativnog goriva za sada u velikoj meri neizvesna.
PB  - Belgrade : Serbian Association of Plant Breedings and Seed Producers
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - The role of maize hybrids in current trends of bioethanol production
VL  - 26
IS  - 2
SP  - 21
EP  - 29
DO  - 10.5937/SelSem2002021N
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Radosavljević, Milica and Simić, Marijana",
year = "2021-01-19",
abstract = "Bioethanol is a biofuel that is mostly used as a replacement for fossil fuels worldwide with
yearly production reaching nearly 110 billion liters in 2019. Trends of producing this alternative
fuel are rising and maize is considered as one of the best renewable raw materials for the production of fuel ethanol due to the high content of starch in the grain. Taking into account that
Serbia is one of the most prominent maize producers in Europe, the surpluses of this crop could
be directed toward bioethanol production. Even though there is no organized production and
consumption of bioethanol as an automotive fuel in Serbia, the Serbian Government has recently
introduced some new regulations regarding biofuels. However, due to the reduction of economic
activities since the onset of the COVID-19 pandemic in 2020, the global demand for crude oil has
fallen sharply, negatively affecting the gasoline demand, and thus bioethanol, which makes the
future of this alternative fuel production notably uncertain., Bioetanol je biogorivo koje se uglavnom koristi kao zamena za fosilna goriva širom sveta,
a godišnja proizvodnja dostigla je gotovo 110 milijardi litara u 2019. godini. Trendovi proizvodnje ovog alternativnog goriva rastu, a kukuruz se smatra jednom od najboljih obnovljivih
sirovina za proizvodnju bioetanola zahvaljujući visokom sadržaju skroba u zrnu. Uzimajući u
obzir da je Srbija jedan od najistaknutijih proizvođača kukuruza u Evropi, viškovi useva ove
poljoprivredne kulture mogli bi se usmeriti ka proizvodnji bioetanola. Iako u Srbiji ne postoji
organizovana proizvodnja i potrošnja bioetanola kao transportnog goriva, Vlada Republike Srbije nedavno je usvojila nekoliko novih uredbi u vezi sa stavljanjem u promet, obeležavanjem i
potrošnjom biogoriva. Međutim, zbog smanjenja ekonomskih aktivnosti od početka pandemije
virusa COVID-19 tokom 2020. godine, globalna potražnja za sirovom naftom naglo je opala, što
je negativno uticalo na potražnju za benzinom, a time i za bioetanolom, zbog čega je budućnost
proizvodnje ovog alternativnog goriva za sada u velikoj meri neizvesna.",
publisher = "Belgrade : Serbian Association of Plant Breedings and Seed Producers, Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "The role of maize hybrids in current trends of bioethanol production",
volume = "26",
number = "2",
pages = "21-29",
doi = "10.5937/SelSem2002021N"
}
Nikolić, V., Žilić, S., Radosavljević, M.,& Simić, M.. (2021-01-19). The role of maize hybrids in current trends of bioethanol production. in Selekcija i semenarstvo
Belgrade : Serbian Association of Plant Breedings and Seed Producers., 26(2), 21-29.
https://doi.org/10.5937/SelSem2002021N
Nikolić V, Žilić S, Radosavljević M, Simić M. The role of maize hybrids in current trends of bioethanol production. in Selekcija i semenarstvo. 2021;26(2):21-29.
doi:10.5937/SelSem2002021N .
Nikolić, Valentina, Žilić, Slađana, Radosavljević, Milica, Simić, Marijana, "The role of maize hybrids in current trends of bioethanol production" in Selekcija i semenarstvo, 26, no. 2 (2021-01-19):21-29,
https://doi.org/10.5937/SelSem2002021N . .
2

Production of maize grain enriched with mineral nutrients in monoculture

Dragičević, Vesna; Simic, Milena; Brankov, Milan; Stoiljkovic, Milovan; Šenk, Milena; Tabaković, Marijenka; Kresović, Branka

(Belgrade : Maize Research Institute, Zemun Polje, 2021)

TY  - CONF
AU  - Dragičević, Vesna
AU  - Simic, Milena
AU  - Brankov, Milan
AU  - Stoiljkovic, Milovan
AU  - Šenk, Milena
AU  - Tabaković, Marijenka
AU  - Kresović, Branka
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/939
AB  - Maize monoculture is still present worldwide. It is an adverse practice, with
high input of agro-chemicals and weed and pathogens infestation. At the
Maize Research Institute, a trial with maize monoculture has started in 1972
and from then it has been progressed into a system designed to maintain soil
fertility, improve grain yield and quality. Experiment (period 2013-2018,
hybrid ZP 427) comprised: treatment with farmyard manure (M -
incorporation of 60 t ha-1 in 2011, 2014, and 2017) and no manure
application (MØ); bio-fertilizer Bioplug (accelerating decomposition of crop
residues) applied at: 5 l ha-1 (BF), 2.5 l ha-1 (1/2BF) and without it (BFØ);
mineral fertilization with N:P:K= 15:15:15 (1709 kg ha-1) in autumn + Urea
(320 kg ha-1), in spring (F1); only Urea (237 kg ha-1) in spring (F2) and no
mineral fertilizers (F3). Measurements included: soil organic matter (OM),
grain yield (after harvest), as well as the content of protein, P, Mg, Ca, Fe
and Zn in grain. Higher soil OM was present in M, F1, and F2 treatments,
particularly in combination with 1/2BF (> 5%, averagely). Greater grain
yield followed the increased level of fertilization: M, BF, and F2 (8.85, 9.17,
and 9.05 t ha-1, respectively, on average). The average protein level, and the
content of mineral nutrients were higher in the S variant, while content of the
protein, P, Mg, and Fe had greater values in the 1/2BF treatment and Ca and
Zn had greater values in BF. Moreover, F1 had positive impact on protein, P,
Ca, and Mg content, while F2 increased the content of Fe and Zn. Results
indicate that proper fertilizer combination: farmyard manure, bio-, and
mineral fertilizer maintained not just soil fertility, but contributed to the
higher grain yield and grain quality, by enhancing the level of protein and
mineral nutrients, even in monoculture, as an adverse practice.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts
T1  - Production of maize grain enriched with mineral nutrients in monoculture
SP  - 62
ER  - 
@conference{
author = "Dragičević, Vesna and Simic, Milena and Brankov, Milan and Stoiljkovic, Milovan and Šenk, Milena and Tabaković, Marijenka and Kresović, Branka",
year = "2021",
abstract = "Maize monoculture is still present worldwide. It is an adverse practice, with
high input of agro-chemicals and weed and pathogens infestation. At the
Maize Research Institute, a trial with maize monoculture has started in 1972
and from then it has been progressed into a system designed to maintain soil
fertility, improve grain yield and quality. Experiment (period 2013-2018,
hybrid ZP 427) comprised: treatment with farmyard manure (M -
incorporation of 60 t ha-1 in 2011, 2014, and 2017) and no manure
application (MØ); bio-fertilizer Bioplug (accelerating decomposition of crop
residues) applied at: 5 l ha-1 (BF), 2.5 l ha-1 (1/2BF) and without it (BFØ);
mineral fertilization with N:P:K= 15:15:15 (1709 kg ha-1) in autumn + Urea
(320 kg ha-1), in spring (F1); only Urea (237 kg ha-1) in spring (F2) and no
mineral fertilizers (F3). Measurements included: soil organic matter (OM),
grain yield (after harvest), as well as the content of protein, P, Mg, Ca, Fe
and Zn in grain. Higher soil OM was present in M, F1, and F2 treatments,
particularly in combination with 1/2BF (> 5%, averagely). Greater grain
yield followed the increased level of fertilization: M, BF, and F2 (8.85, 9.17,
and 9.05 t ha-1, respectively, on average). The average protein level, and the
content of mineral nutrients were higher in the S variant, while content of the
protein, P, Mg, and Fe had greater values in the 1/2BF treatment and Ca and
Zn had greater values in BF. Moreover, F1 had positive impact on protein, P,
Ca, and Mg content, while F2 increased the content of Fe and Zn. Results
indicate that proper fertilizer combination: farmyard manure, bio-, and
mineral fertilizer maintained not just soil fertility, but contributed to the
higher grain yield and grain quality, by enhancing the level of protein and
mineral nutrients, even in monoculture, as an adverse practice.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts",
title = "Production of maize grain enriched with mineral nutrients in monoculture",
pages = "62"
}
Dragičević, V., Simic, M., Brankov, M., Stoiljkovic, M., Šenk, M., Tabaković, M.,& Kresović, B.. (2021). Production of maize grain enriched with mineral nutrients in monoculture. in International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 62.
Dragičević V, Simic M, Brankov M, Stoiljkovic M, Šenk M, Tabaković M, Kresović B. Production of maize grain enriched with mineral nutrients in monoculture. in International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts. 2021;:62..
Dragičević, Vesna, Simic, Milena, Brankov, Milan, Stoiljkovic, Milovan, Šenk, Milena, Tabaković, Marijenka, Kresović, Branka, "Production of maize grain enriched with mineral nutrients in monoculture" in International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts (2021):62.

Biofortification as a way of nutrient dense feed production

Dragičević, Vesna; Simic, Milena; Brankov, Milan; Šenk, Milena; Krnjaja, Vesna; Mandić, Violeta; Kresović, Branka

(Belgrade : Institute for Animal Husbandry, 2021)

TY  - CONF
AU  - Dragičević, Vesna
AU  - Simic, Milena
AU  - Brankov, Milan
AU  - Šenk, Milena
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Kresović, Branka
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/941
AB  - Malnutrition is a global problem, hitting both, people and
animals. Due to the many factors, such as climate change, soil depletion,
anthropogenic impact, including irresponsible soil management, high fertilization
rates with macro-nutrients, crops lack in essential nutrients, particularly minerals,
Mg, Fe, Zn, etc., as well as important vitamins, like carotenoids, vitamins from B
group. Also, some arable soils have naturally low fertility. All of that negatively
affect production of domestic animal, including animal health and quality of
livestock products. To mitigate malnutrition, a bio-fortification strategy was
developed. It is based on increase in the concentration of essential nutrients in food
and feed, and also to promote further bio-availability from digestive organs. Biofortification implies various practices. Two main types of bio-fortification were
developed: genetic bio-fortification, and agronomic bio-fortification. The first one
uses standard breeding techniques, marker assistant selection, transgenic
approaches, genome editing, etc., to develop highly-efficient genotypes, that are
able to absorb and accumulate higher concentrations of essential nutrients in
biomass and grains. Agronomic bio-fortification uses different practices, such is
special fertilizers, enriched with essential minerals, foliar fertilizers, bio-fertilizers,
growth hormones and enhancers, and some lesser known sustainable practices, like
inter-cropping, cover cropping, in order to increase crop yields, as well as the
concentration of essential nutrients. The bio-fortification approach, based on the
development and commercialisation of highly efficient genotypes, as well as
agricultural practices that enable and support better absorption and accumulation of
essential nutrients option that is safer for both, people and domestic animals.
Mutual increase in concentration of essential minerals, vitamins, and other
promoting substances is of a particular importance for bio-fortification programs,
increasing efficiency and success of applied practices, thus positively reflecting on
animal health and wellbeing.
AB  - Neishranjenost predstavlja globalni problem, pogađajući i ljude i domaće životinje,
paralelno. Zahvaljujući broujnim faktorima, kao što su promena klime,
ispošćavanje zemljišta, uticaj antropogenog faktora preko neodgovornog
upravljanja zemljištem, visokih unosa mineralnih đubriva baziranih na makroelementima, u usevima se javlja se nedostatak esencijalnih hraniva, kao što su Mg,
Fe, Zn, itd., kao i važnih vitamina, kao što su karotenoidi i vitamini iz B grupe.
Takođe, neke obradive površine imaju prirodno nisku plodnost. Sve navedeno se
negativno odražava na proizvodnju domaćih životinja, uključujući zdravlje
životinja, kao i kvalitet životinjskih proizvoda. Kao odgovor u borbi protiv
neishranjenosti, razvijena je strategija bio-fortifikacije, koja se bazira na povećanju
koncentracije esencijalnih hraniva u hrani i hranivima, kao i većoj pristupačnosti iz
organa za varenje. Bio-fortifikacija koristi različite mere. Dva osnovna tipa biofortifikacije su razvijena: genetička i agronomska bio-fortifikacija. Prva koristi
stndardne tehnike selekcije, marker asastiranu selekciju, transgene pristupe,
editovanje genoma, i dr. u dobijanju vioko-efikasnih genotipova koji su sposobni
da apsorbuju i akumuliraju esencijana hraniva u većim koncentracijama u biomasi i
zrnu. Agrnonomska bio-fortifikacija koristi različite tehnike, kao što je primena
specijalnih đubriva obogaćenih sa esencijalnim mineralima, folijarnih đubriva, hormona i poboljšivača rasta, kao i nekih manje poznatih mera koje se koriste u
održivoj poljoprivredi, kao što su kombinovani i pokrovni usevi, koji su
prvenstveno namenjeni povećanju prinosa, kao i koncentracije esencijalnih hraniva.
Strategija bio-fortifikacije, bazirana na razvijanju i komercijalizaciji visoko
efikasnih genotipova, kao i agronomskih tehnika, koje omogućavaju i podržavaju
bolje usvajanje i akumulaciju esencijanih hraniva je bezbednija opcija i za ljude i
za domaće životinje. Paralelno povećanje koncentracije esentijalnih minerala,
vitamina i drugih supstanci koje pomažu usvajanje je od posebnog značaja za
programe bio-fortifikacije, povećavajući efikasnost i uspešnost primenjenih mera, i
odražavajući se pozitivnona zdravlje i blagostanje domaćih životinja.
PB  - Belgrade : Institute for Animal Husbandry
C3  - 13. International symposium “Modern trends in livestock production”, 6-8. October 2021., Belgrade, Serbia - Proceedings
T1  - Biofortification as a way of nutrient dense feed production
T1  - Biofortifikacija, kao način proizvodnje nutritivno bogate hrane za domaće životinje
SP  - 287
EP  - 307
ER  - 
@conference{
author = "Dragičević, Vesna and Simic, Milena and Brankov, Milan and Šenk, Milena and Krnjaja, Vesna and Mandić, Violeta and Kresović, Branka",
year = "2021",
abstract = "Malnutrition is a global problem, hitting both, people and
animals. Due to the many factors, such as climate change, soil depletion,
anthropogenic impact, including irresponsible soil management, high fertilization
rates with macro-nutrients, crops lack in essential nutrients, particularly minerals,
Mg, Fe, Zn, etc., as well as important vitamins, like carotenoids, vitamins from B
group. Also, some arable soils have naturally low fertility. All of that negatively
affect production of domestic animal, including animal health and quality of
livestock products. To mitigate malnutrition, a bio-fortification strategy was
developed. It is based on increase in the concentration of essential nutrients in food
and feed, and also to promote further bio-availability from digestive organs. Biofortification implies various practices. Two main types of bio-fortification were
developed: genetic bio-fortification, and agronomic bio-fortification. The first one
uses standard breeding techniques, marker assistant selection, transgenic
approaches, genome editing, etc., to develop highly-efficient genotypes, that are
able to absorb and accumulate higher concentrations of essential nutrients in
biomass and grains. Agronomic bio-fortification uses different practices, such is
special fertilizers, enriched with essential minerals, foliar fertilizers, bio-fertilizers,
growth hormones and enhancers, and some lesser known sustainable practices, like
inter-cropping, cover cropping, in order to increase crop yields, as well as the
concentration of essential nutrients. The bio-fortification approach, based on the
development and commercialisation of highly efficient genotypes, as well as
agricultural practices that enable and support better absorption and accumulation of
essential nutrients option that is safer for both, people and domestic animals.
Mutual increase in concentration of essential minerals, vitamins, and other
promoting substances is of a particular importance for bio-fortification programs,
increasing efficiency and success of applied practices, thus positively reflecting on
animal health and wellbeing., Neishranjenost predstavlja globalni problem, pogađajući i ljude i domaće životinje,
paralelno. Zahvaljujući broujnim faktorima, kao što su promena klime,
ispošćavanje zemljišta, uticaj antropogenog faktora preko neodgovornog
upravljanja zemljištem, visokih unosa mineralnih đubriva baziranih na makroelementima, u usevima se javlja se nedostatak esencijalnih hraniva, kao što su Mg,
Fe, Zn, itd., kao i važnih vitamina, kao što su karotenoidi i vitamini iz B grupe.
Takođe, neke obradive površine imaju prirodno nisku plodnost. Sve navedeno se
negativno odražava na proizvodnju domaćih životinja, uključujući zdravlje
životinja, kao i kvalitet životinjskih proizvoda. Kao odgovor u borbi protiv
neishranjenosti, razvijena je strategija bio-fortifikacije, koja se bazira na povećanju
koncentracije esencijalnih hraniva u hrani i hranivima, kao i većoj pristupačnosti iz
organa za varenje. Bio-fortifikacija koristi različite mere. Dva osnovna tipa biofortifikacije su razvijena: genetička i agronomska bio-fortifikacija. Prva koristi
stndardne tehnike selekcije, marker asastiranu selekciju, transgene pristupe,
editovanje genoma, i dr. u dobijanju vioko-efikasnih genotipova koji su sposobni
da apsorbuju i akumuliraju esencijana hraniva u većim koncentracijama u biomasi i
zrnu. Agrnonomska bio-fortifikacija koristi različite tehnike, kao što je primena
specijalnih đubriva obogaćenih sa esencijalnim mineralima, folijarnih đubriva, hormona i poboljšivača rasta, kao i nekih manje poznatih mera koje se koriste u
održivoj poljoprivredi, kao što su kombinovani i pokrovni usevi, koji su
prvenstveno namenjeni povećanju prinosa, kao i koncentracije esencijalnih hraniva.
Strategija bio-fortifikacije, bazirana na razvijanju i komercijalizaciji visoko
efikasnih genotipova, kao i agronomskih tehnika, koje omogućavaju i podržavaju
bolje usvajanje i akumulaciju esencijanih hraniva je bezbednija opcija i za ljude i
za domaće životinje. Paralelno povećanje koncentracije esentijalnih minerala,
vitamina i drugih supstanci koje pomažu usvajanje je od posebnog značaja za
programe bio-fortifikacije, povećavajući efikasnost i uspešnost primenjenih mera, i
odražavajući se pozitivnona zdravlje i blagostanje domaćih životinja.",
publisher = "Belgrade : Institute for Animal Husbandry",
journal = "13. International symposium “Modern trends in livestock production”, 6-8. October 2021., Belgrade, Serbia - Proceedings",
title = "Biofortification as a way of nutrient dense feed production, Biofortifikacija, kao način proizvodnje nutritivno bogate hrane za domaće životinje",
pages = "287-307"
}
Dragičević, V., Simic, M., Brankov, M., Šenk, M., Krnjaja, V., Mandić, V.,& Kresović, B.. (2021). Biofortification as a way of nutrient dense feed production. in 13. International symposium “Modern trends in livestock production”, 6-8. October 2021., Belgrade, Serbia - Proceedings
Belgrade : Institute for Animal Husbandry., 287-307.
Dragičević V, Simic M, Brankov M, Šenk M, Krnjaja V, Mandić V, Kresović B. Biofortification as a way of nutrient dense feed production. in 13. International symposium “Modern trends in livestock production”, 6-8. October 2021., Belgrade, Serbia - Proceedings. 2021;:287-307..
Dragičević, Vesna, Simic, Milena, Brankov, Milan, Šenk, Milena, Krnjaja, Vesna, Mandić, Violeta, Kresović, Branka, "Biofortification as a way of nutrient dense feed production" in 13. International symposium “Modern trends in livestock production”, 6-8. October 2021., Belgrade, Serbia - Proceedings (2021):287-307.

Impact of soybean-proso millet intercropping on productivity and micronutrient accumulation in biomass

Šenk, Milena; Simic, Milena; Milojković-Opsenica, Dušanka; Brankov, Milan; Djurdjic, Sladjana; Dragičević, Vesna

(Belgrade : Maize Research Institute, Zemun Polje, 2021)

TY  - CONF
AU  - Šenk, Milena
AU  - Simic, Milena
AU  - Milojković-Opsenica, Dušanka
AU  - Brankov, Milan
AU  - Djurdjic, Sladjana
AU  - Dragičević, Vesna
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/940
AB  - Intercropping, as one of the most beneficial sustainable agriculture practices that increase biodiversity, includes planting of two or more crops simultaneously at the same field. Advantages of intercropping are reflected through the more efficient use of natural resources by the complementary crops, resulting in increased yield and improved biomass quality. One of the most useful ways to express the impact of intercropping is land equivalent ratio (LER) as well as nutrient land equivalent ratio (NLER). The aim of this research was to examine the influence of different intercrop combinations of soybean and proso millet on biomass yield and concentration of essential minerals (Fe, Zn), shown as LER, Fe-LER and Zn-LER. Soybean and proso millet were sown in three combinations (S-M, SS-MM and SS-MMMM), as well as sole crops, in 2018 and 2020. The effect of bio-fertilizer Coveron (containing Glomus sp. and Trichoderma) was also investigated. The results showed that SS-MM and SS-MMMM combinations contributed to greater accumulation of Fe in biomass of soybean and proso millet. In regard to Zn concentration, situation was opposite. While all intercrop combinations expressed positive effect on the accumulation of this element in soybean, more Zn was absorbed by proso grown as sole crop in relation to the intercrops. Fe-LER and Zn-LER values showed that year had no significant impact, while the influence of intercropping had significantly highlighted SS-MM combination (the highest values were 1.20 and 1.25 for Fe and Zn, respectively). The effect of bio-fertilizer could be observed just in the case of Fe-LER, where Coveron expressed negative impact. Similarly, the LER value for yield was only in the SS-MM combination >1. Based on these results, it can be concluded that soybean-proso millet intercropping had beneficial effect on productivity and micronutrient absorption by biomass, emphasizing SS-MM combination as the most efficient.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts
T1  - Impact of soybean-proso millet intercropping on productivity and micronutrient accumulation in biomass
SP  - 79
ER  - 
@conference{
author = "Šenk, Milena and Simic, Milena and Milojković-Opsenica, Dušanka and Brankov, Milan and Djurdjic, Sladjana and Dragičević, Vesna",
year = "2021",
abstract = "Intercropping, as one of the most beneficial sustainable agriculture practices that increase biodiversity, includes planting of two or more crops simultaneously at the same field. Advantages of intercropping are reflected through the more efficient use of natural resources by the complementary crops, resulting in increased yield and improved biomass quality. One of the most useful ways to express the impact of intercropping is land equivalent ratio (LER) as well as nutrient land equivalent ratio (NLER). The aim of this research was to examine the influence of different intercrop combinations of soybean and proso millet on biomass yield and concentration of essential minerals (Fe, Zn), shown as LER, Fe-LER and Zn-LER. Soybean and proso millet were sown in three combinations (S-M, SS-MM and SS-MMMM), as well as sole crops, in 2018 and 2020. The effect of bio-fertilizer Coveron (containing Glomus sp. and Trichoderma) was also investigated. The results showed that SS-MM and SS-MMMM combinations contributed to greater accumulation of Fe in biomass of soybean and proso millet. In regard to Zn concentration, situation was opposite. While all intercrop combinations expressed positive effect on the accumulation of this element in soybean, more Zn was absorbed by proso grown as sole crop in relation to the intercrops. Fe-LER and Zn-LER values showed that year had no significant impact, while the influence of intercropping had significantly highlighted SS-MM combination (the highest values were 1.20 and 1.25 for Fe and Zn, respectively). The effect of bio-fertilizer could be observed just in the case of Fe-LER, where Coveron expressed negative impact. Similarly, the LER value for yield was only in the SS-MM combination >1. Based on these results, it can be concluded that soybean-proso millet intercropping had beneficial effect on productivity and micronutrient absorption by biomass, emphasizing SS-MM combination as the most efficient.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts",
title = "Impact of soybean-proso millet intercropping on productivity and micronutrient accumulation in biomass",
pages = "79"
}
Šenk, M., Simic, M., Milojković-Opsenica, D., Brankov, M., Djurdjic, S.,& Dragičević, V.. (2021). Impact of soybean-proso millet intercropping on productivity and micronutrient accumulation in biomass. in International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 79.
Šenk M, Simic M, Milojković-Opsenica D, Brankov M, Djurdjic S, Dragičević V. Impact of soybean-proso millet intercropping on productivity and micronutrient accumulation in biomass. in International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts. 2021;:79..
Šenk, Milena, Simic, Milena, Milojković-Opsenica, Dušanka, Brankov, Milan, Djurdjic, Sladjana, Dragičević, Vesna, "Impact of soybean-proso millet intercropping on productivity and micronutrient accumulation in biomass" in International conference “The frontiers of science and technology in crop breeding and production conference”, 8-9. June 2021., Belgrade, Serbia - Book of abstracts (2021):79.

Статус појединих макроелемената у кукурузу различите боје зрна

Dragičević, Vesna; Simić, Milena; Brankov, Milan; Stoiljković, Milovan; Delić, Nenad; Tolimir, Miodrag; Šenk, Milena

(Beograd : Univerzitet u Beogradu - Poljoprivredni fakultet, 2021)

TY  - CONF
AU  - Dragičević, Vesna
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Stoiljković, Milovan
AU  - Delić, Nenad
AU  - Tolimir, Miodrag
AU  - Šenk, Milena
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/942
AB  - Зрно кукуруза је важан извор минералних елемената у људској исхрани. Разлике у
садржају минерала у зрну кукуруза могу зависити од генотипа, система гајења,
примене ђубрива, као и метеоролошких услова. Циљ експеримента је био да се испита
утицај различитих система ђубрења (минерално ђубриво – уреа, органско ђубриво –
Fertor, микробиолошко ђубриво – Team micoriza plus, контрола – без ђубрења) на
принос и промене у садржају P, S, Ca, Mg и Fe у кукурузу белог, жутог и црвеног зрна,
током вегетационе сезоне 2017. и 2018. године. У погледу метеоролошких услова,
уочен је сушан период током јуна–августа 2017., док је 2018. имала равномеран
распоред падавина. Зато је у 2017. просечан принос зрна био скоро двоструко мањи, за
4,6 t ha-1, у односу на 2018. годину, али је у 2017. била знатно виша просечна
концентрација Ca, Mg, S и Fе. Зрно црвеног кукуруза је уз просечно већи принос, било
богатије у садржају Cа, Fе и P, док је, органско ђубриво у највећој мери утицало на
повећање приноса и акумулацију Mg, P и S. Код кукуруза црвеног зрна примена
органског ђубрива је у највећем степену довела до већег приноса, као и концентрације
Mg и P, док је микробиолошко ђубриво повећало концентрацију Ca, а уреа Fе. Једино
је већи ниво S био забележен у контроли код кукуруза жуте боје зрна. Највећа
варирања вредности приноса била су код жутог кукуруза (третман са органским
ђубривом и контрола) и црвеног у третману са уреом, док су највећа варирања P, S, Mg
и Fе била код кукуруза црвеног зрна (третман са микробилошким и органским
ђубривом), као и кукуруза жутог зрна у контроли. Приказани резултати указују да се
преко услова гајења може утицати на накупљање важних минерала у зрну кукуруза и
то посебно црвене боје, које би се стога могло сматрати важним извором P, Cа, Mg и
Fе, док би жуто зрно кукуруза могло представљати значајан извор S.
AB  - Maize kernel is an important source of mineral elements in human nutrition. Differences in
mineral content in maize kernel depend on genotype, cropping systems, fertilization, and
meteorological conditions. The objective of this study was to examine the impact of different
fertilization systems (mineral fertilizer – urea, organic fertilizer – Fertor, biofertilizer – Team
micoriza plus, control – without fertilization) on kernel yield and variations in content of P, S,
Ca, Mg, and Fe in kernel of white, yellow and red maize, during 2017 and 2018.
Meteorological conditions indicated dry period during June–August 2017, while in 2018
precipitations were equally distributed. Therefore, the average yield was almost double lower
in 2017 (to 4.6 t ha-1), compared to 2018, while higher average concentration of Ca, Mg, S,
and Fe in maize kernels was recorded in 2017. With higher average yield, red maize kernel
was richer in Ca, Fe, and P. Organic fertilizer increased average yield, and accumulation of
Mg, P, and S in kernels. In regard to combinations, application of organic fertilizer in red
maize resulted in increase of yield, Mg and P, while the biofertilizer increased Ca and urea
increased Fe concentration in kernel. S concentration achieved the highest value in the
control in yellow maize kernel. The greatest variations of kernel yield were in yellow maize
(treatment with the organic fertilizer and control) and in red maize with the urea treatment,
while the greatest variability of P, S, Mg, and Fe concentration was in red maize kernel
(treatment with the bio- and organic fertilizer), as well as yellow maize kernel in the control.
Presented results indicated that adjustments in growing conditions could impact accumulation
of important minerals in kernel, particularly in red maize, which could be considered as a
significant source of P, CA, Mg, and Fe, while yellow maize kernel could be referred as a
significant source of S.
PB  - Beograd : Univerzitet u Beogradu - Poljoprivredni fakultet
C3  - 10. Simpozijum sa međunarodnim učešćem "Inovacije u ratarskoj i povrtarskoj proizvodnji", 21-22. oktobar 2021., Beograd - Zbornik izvoda
T1  - Статус појединих макроелемената у кукурузу различите боје зрна
T1  - Status of some macro-elements in maize kernel with different colour
SP  - 66
EP  - 67
ER  - 
@conference{
author = "Dragičević, Vesna and Simić, Milena and Brankov, Milan and Stoiljković, Milovan and Delić, Nenad and Tolimir, Miodrag and Šenk, Milena",
year = "2021",
abstract = "Зрно кукуруза је важан извор минералних елемената у људској исхрани. Разлике у
садржају минерала у зрну кукуруза могу зависити од генотипа, система гајења,
примене ђубрива, као и метеоролошких услова. Циљ експеримента је био да се испита
утицај различитих система ђубрења (минерално ђубриво – уреа, органско ђубриво –
Fertor, микробиолошко ђубриво – Team micoriza plus, контрола – без ђубрења) на
принос и промене у садржају P, S, Ca, Mg и Fe у кукурузу белог, жутог и црвеног зрна,
током вегетационе сезоне 2017. и 2018. године. У погледу метеоролошких услова,
уочен је сушан период током јуна–августа 2017., док је 2018. имала равномеран
распоред падавина. Зато је у 2017. просечан принос зрна био скоро двоструко мањи, за
4,6 t ha-1, у односу на 2018. годину, али је у 2017. била знатно виша просечна
концентрација Ca, Mg, S и Fе. Зрно црвеног кукуруза је уз просечно већи принос, било
богатије у садржају Cа, Fе и P, док је, органско ђубриво у највећој мери утицало на
повећање приноса и акумулацију Mg, P и S. Код кукуруза црвеног зрна примена
органског ђубрива је у највећем степену довела до већег приноса, као и концентрације
Mg и P, док је микробиолошко ђубриво повећало концентрацију Ca, а уреа Fе. Једино
је већи ниво S био забележен у контроли код кукуруза жуте боје зрна. Највећа
варирања вредности приноса била су код жутог кукуруза (третман са органским
ђубривом и контрола) и црвеног у третману са уреом, док су највећа варирања P, S, Mg
и Fе била код кукуруза црвеног зрна (третман са микробилошким и органским
ђубривом), као и кукуруза жутог зрна у контроли. Приказани резултати указују да се
преко услова гајења може утицати на накупљање важних минерала у зрну кукуруза и
то посебно црвене боје, које би се стога могло сматрати важним извором P, Cа, Mg и
Fе, док би жуто зрно кукуруза могло представљати значајан извор S., Maize kernel is an important source of mineral elements in human nutrition. Differences in
mineral content in maize kernel depend on genotype, cropping systems, fertilization, and
meteorological conditions. The objective of this study was to examine the impact of different
fertilization systems (mineral fertilizer – urea, organic fertilizer – Fertor, biofertilizer – Team
micoriza plus, control – without fertilization) on kernel yield and variations in content of P, S,
Ca, Mg, and Fe in kernel of white, yellow and red maize, during 2017 and 2018.
Meteorological conditions indicated dry period during June–August 2017, while in 2018
precipitations were equally distributed. Therefore, the average yield was almost double lower
in 2017 (to 4.6 t ha-1), compared to 2018, while higher average concentration of Ca, Mg, S,
and Fe in maize kernels was recorded in 2017. With higher average yield, red maize kernel
was richer in Ca, Fe, and P. Organic fertilizer increased average yield, and accumulation of
Mg, P, and S in kernels. In regard to combinations, application of organic fertilizer in red
maize resulted in increase of yield, Mg and P, while the biofertilizer increased Ca and urea
increased Fe concentration in kernel. S concentration achieved the highest value in the
control in yellow maize kernel. The greatest variations of kernel yield were in yellow maize
(treatment with the organic fertilizer and control) and in red maize with the urea treatment,
while the greatest variability of P, S, Mg, and Fe concentration was in red maize kernel
(treatment with the bio- and organic fertilizer), as well as yellow maize kernel in the control.
Presented results indicated that adjustments in growing conditions could impact accumulation
of important minerals in kernel, particularly in red maize, which could be considered as a
significant source of P, CA, Mg, and Fe, while yellow maize kernel could be referred as a
significant source of S.",
publisher = "Beograd : Univerzitet u Beogradu - Poljoprivredni fakultet",
journal = "10. Simpozijum sa međunarodnim učešćem "Inovacije u ratarskoj i povrtarskoj proizvodnji", 21-22. oktobar 2021., Beograd - Zbornik izvoda",
title = "Статус појединих макроелемената у кукурузу различите боје зрна, Status of some macro-elements in maize kernel with different colour",
pages = "66-67"
}
Dragičević, V., Simić, M., Brankov, M., Stoiljković, M., Delić, N., Tolimir, M.,& Šenk, M.. (2021). Статус појединих макроелемената у кукурузу различите боје зрна. in 10. Simpozijum sa međunarodnim učešćem "Inovacije u ratarskoj i povrtarskoj proizvodnji", 21-22. oktobar 2021., Beograd - Zbornik izvoda
Beograd : Univerzitet u Beogradu - Poljoprivredni fakultet., 66-67.
Dragičević V, Simić M, Brankov M, Stoiljković M, Delić N, Tolimir M, Šenk M. Статус појединих макроелемената у кукурузу различите боје зрна. in 10. Simpozijum sa međunarodnim učešćem "Inovacije u ratarskoj i povrtarskoj proizvodnji", 21-22. oktobar 2021., Beograd - Zbornik izvoda. 2021;:66-67..
Dragičević, Vesna, Simić, Milena, Brankov, Milan, Stoiljković, Milovan, Delić, Nenad, Tolimir, Miodrag, Šenk, Milena, "Статус појединих макроелемената у кукурузу различите боје зрна" in 10. Simpozijum sa međunarodnim učešćem "Inovacije u ratarskoj i povrtarskoj proizvodnji", 21-22. oktobar 2021., Beograd - Zbornik izvoda (2021):66-67.

Adapted quality protein maize for broiler feeds

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Delić, Nenad

(Belgrade : Maize Research Institute, Zemun Polje, 2021)

TY  - GEN
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/969
AB  - Maize has low nutritional quality due to poor content of essential amino
acids lysine and tryptophan in the dominant seed storage protein fraction,
zeins. However, Quality Protein Maize (QPM), a variety of opaque2 maize
but with good agronomic traits, can have 60% to 100% higher content of
lysine than standard maize. Monogastric animals (pigs, poultry, fish), like
humans, cannot synthesize lysine and tryptophan de novo and thus these
amino acids must be supplied through diets. QPM was primarily developed
for human consumption to overcome malnutrition in countries where maize
is staple food. Thus, QPM is of tropical origin and its adaptation to temperate
regions is frequently hampered by the retained exotic germplasm.
Development of adapted QPM hybrids is mainly aimed for feed industry. It
has been shown that substituting standard maize with QPM in feed diets
could be profitable due to improved weight gain, feed conversion ratio and
decreasing of dietary lysine supplementation. Maize Research Institute,
Zemun Polje has a program on converting elite inbred lines through marker
assisted breeding into their QPM counterparts and developing commercial
QPM hybrids. Up to now, two inbred lines have been converted, while eight
lines have passed through three or four generations of backcrossing and one
generation of selfing. Tryptophan content in the converted selfed plants was
in the range from 0.080 to 0.093. Besides developing QPM counterparts of
commercial hybrids, one QPM hybrid obtained by crossing a converted elite
inbred line and an adapted tropical inbred line was developed and tested over
different locations and in different years. This hybrid is currently used in
feeding experiments with the objective to test effects of replacing standard
maize with QPM in diets on broiler performances.
PB  - Belgrade : Maize Research Institute, Zemun Polje
T2  - International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts
T1  - Adapted quality protein maize for broiler feeds
SP  - 75
ER  - 
@misc{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad",
year = "2021",
abstract = "Maize has low nutritional quality due to poor content of essential amino
acids lysine and tryptophan in the dominant seed storage protein fraction,
zeins. However, Quality Protein Maize (QPM), a variety of opaque2 maize
but with good agronomic traits, can have 60% to 100% higher content of
lysine than standard maize. Monogastric animals (pigs, poultry, fish), like
humans, cannot synthesize lysine and tryptophan de novo and thus these
amino acids must be supplied through diets. QPM was primarily developed
for human consumption to overcome malnutrition in countries where maize
is staple food. Thus, QPM is of tropical origin and its adaptation to temperate
regions is frequently hampered by the retained exotic germplasm.
Development of adapted QPM hybrids is mainly aimed for feed industry. It
has been shown that substituting standard maize with QPM in feed diets
could be profitable due to improved weight gain, feed conversion ratio and
decreasing of dietary lysine supplementation. Maize Research Institute,
Zemun Polje has a program on converting elite inbred lines through marker
assisted breeding into their QPM counterparts and developing commercial
QPM hybrids. Up to now, two inbred lines have been converted, while eight
lines have passed through three or four generations of backcrossing and one
generation of selfing. Tryptophan content in the converted selfed plants was
in the range from 0.080 to 0.093. Besides developing QPM counterparts of
commercial hybrids, one QPM hybrid obtained by crossing a converted elite
inbred line and an adapted tropical inbred line was developed and tested over
different locations and in different years. This hybrid is currently used in
feeding experiments with the objective to test effects of replacing standard
maize with QPM in diets on broiler performances.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts",
title = "Adapted quality protein maize for broiler feeds",
pages = "75"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J.,& Delić, N.. (2021). Adapted quality protein maize for broiler feeds. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 75.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Delić N. Adapted quality protein maize for broiler feeds. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts. 2021;:75..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, "Adapted quality protein maize for broiler feeds" in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts (2021):75.

Biochemical characterization of soybean genotypes for grain quality improvement

Ristić, Danijela; Perić, Vesna; Srebrić, Mirjana; Kostadinović, Marija; Kravić, Natalija; Kovinčić, Anika; Gošić-Dondo, Snežana

(Belgrade ; Maize Research Institute, Zemun Polje, 2021)

TY  - CONF
AU  - Ristić, Danijela
AU  - Perić, Vesna
AU  - Srebrić, Mirjana
AU  - Kostadinović, Marija
AU  - Kravić, Natalija
AU  - Kovinčić, Anika
AU  - Gošić-Dondo, Snežana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/966
AB  - Soybean is one of the most economically important grain crops. As the
source of plant protein and vegetable oil, it is widely used as food and
industrial crop. Quality of soybean oil is determined by fatty acid (FA)
composition. Biochemical variation of different varieties significantly
facilitates genetic screening of kernel traits and grain quality attributes, thus
leading to one of the most important breeding strategies - improvement of
the grain composition. The main objective of this work was to evaluate
whole kernel biochemical composition of eight soybean varieties and eight
lines from different breeding programs. Phytochemical characterization was
conducted for total protein and oil content, as well as for fatty acids content
(e.g. palmitic, stearic, oleic, linoleic and linolenic acid). According to
Kjedahl method, total protein content ranged from 37.01% to 39.91%. The
obtained results showed that the lowest and the highest values for oil content
were 19.00% and 21.34%, respectively. Gas Chromatography/Flame
Ionization Detector (GC-FID) was used for FA quantification and
determination. The most abundant FA was linoleic acid with a range from
47.45 to 55.7 g/100g, followed by oleic acid with detected rage from 22.59
to 29.07 g/100g of total FA content. Palmitic, linolenic and stearic FA
showed a significantly lower level varied from 9.57 to 11.51 g/100g, 5.42 to
7.18 g/100g and 3.73 to 5.86 g/100g, respectively. This variability observed
in kernel quality among soybean genotypes can provide valuable information
for future breeding.
PB  - Belgrade ;  Maize Research Institute, Zemun Polje
C3  - International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia
T1  - Biochemical characterization of soybean genotypes for grain quality improvement
SP  - 77
ER  - 
@conference{
author = "Ristić, Danijela and Perić, Vesna and Srebrić, Mirjana and Kostadinović, Marija and Kravić, Natalija and Kovinčić, Anika and Gošić-Dondo, Snežana",
year = "2021",
abstract = "Soybean is one of the most economically important grain crops. As the
source of plant protein and vegetable oil, it is widely used as food and
industrial crop. Quality of soybean oil is determined by fatty acid (FA)
composition. Biochemical variation of different varieties significantly
facilitates genetic screening of kernel traits and grain quality attributes, thus
leading to one of the most important breeding strategies - improvement of
the grain composition. The main objective of this work was to evaluate
whole kernel biochemical composition of eight soybean varieties and eight
lines from different breeding programs. Phytochemical characterization was
conducted for total protein and oil content, as well as for fatty acids content
(e.g. palmitic, stearic, oleic, linoleic and linolenic acid). According to
Kjedahl method, total protein content ranged from 37.01% to 39.91%. The
obtained results showed that the lowest and the highest values for oil content
were 19.00% and 21.34%, respectively. Gas Chromatography/Flame
Ionization Detector (GC-FID) was used for FA quantification and
determination. The most abundant FA was linoleic acid with a range from
47.45 to 55.7 g/100g, followed by oleic acid with detected rage from 22.59
to 29.07 g/100g of total FA content. Palmitic, linolenic and stearic FA
showed a significantly lower level varied from 9.57 to 11.51 g/100g, 5.42 to
7.18 g/100g and 3.73 to 5.86 g/100g, respectively. This variability observed
in kernel quality among soybean genotypes can provide valuable information
for future breeding.",
publisher = "Belgrade ;  Maize Research Institute, Zemun Polje",
journal = "International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia",
title = "Biochemical characterization of soybean genotypes for grain quality improvement",
pages = "77"
}
Ristić, D., Perić, V., Srebrić, M., Kostadinović, M., Kravić, N., Kovinčić, A.,& Gošić-Dondo, S.. (2021). Biochemical characterization of soybean genotypes for grain quality improvement. in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia
Belgrade ;  Maize Research Institute, Zemun Polje., 77.
Ristić D, Perić V, Srebrić M, Kostadinović M, Kravić N, Kovinčić A, Gošić-Dondo S. Biochemical characterization of soybean genotypes for grain quality improvement. in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia. 2021;:77..
Ristić, Danijela, Perić, Vesna, Srebrić, Mirjana, Kostadinović, Marija, Kravić, Natalija, Kovinčić, Anika, Gošić-Dondo, Snežana, "Biochemical characterization of soybean genotypes for grain quality improvement" in International conference the frontiers of science and technology in crop breeding and production,  08 - 09.06.2021; Belgrade, Serbia (2021):77.

Direct and Joint Effects of Genotype, Defoliation and Crop Density on the Yield of Three Inbred Maize Lines

Ranković, Dejan; Todorović, Goran; Tabaković, Marijenka; Prodanović, Slaven; Boćanski, Jan; Delić, Nenad

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Ranković, Dejan
AU  - Todorović, Goran
AU  - Tabaković, Marijenka
AU  - Prodanović, Slaven
AU  - Boćanski, Jan
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/850
AB  - The aim of this study was to observe direct and joint effects of three factors (genotypes,
ecological environmental conditions and the applied crop density) on the level of defoliation intensity
and yield. Three inbred lines (G) of maize (G1–L217RfC, G2–L335/99 and G3–L76B004) were used
in the study. The trials were performed in two years (Y) (Y1 = 2016 and Y2 = 2017) and in two
locations (L) (L1 and L2) under four ecological conditions of the year–location interaction (E1–E4)
and in two densities (D1 and D2) (50,000 and 65,000 plants ha−1
). Prior to tasselling, the following
five treatments of detasseling and defoliation (T) were applied: T1—control, no leaf removal only
detasseling, T2–T5—removal of tassels and top leaves (from one to four top leaves). The defoliation
treatments had the most pronounced effect on the yield reduction in G1 (T1–Tn+1 . . . T5), p < 0.05.
The ecological conditions on yield variability were expressed under poor weather conditions (E3 and
E4), while lower densities were less favorable for the application of defoliation treatments. The result
of joint effects of factors was the lowest grain yield (896 kg/ha) in G3 in the variant E3D1 for T2 and
the highest grain yield (11,389 kg/ha) in G3 in the variant E2D2 for T1. The smallest effect of the
defoliation treatment was on the kernel row number (KRN)
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Agriculture
T1  - Direct and Joint Effects of Genotype, Defoliation and Crop Density on the Yield of Three Inbred Maize Lines
VL  - 11
IS  - 6
SP  - 509
DO  - 10.3390/ agriculture11060509
ER  - 
@article{
author = "Ranković, Dejan and Todorović, Goran and Tabaković, Marijenka and Prodanović, Slaven and Boćanski, Jan and Delić, Nenad",
year = "2021",
abstract = "The aim of this study was to observe direct and joint effects of three factors (genotypes,
ecological environmental conditions and the applied crop density) on the level of defoliation intensity
and yield. Three inbred lines (G) of maize (G1–L217RfC, G2–L335/99 and G3–L76B004) were used
in the study. The trials were performed in two years (Y) (Y1 = 2016 and Y2 = 2017) and in two
locations (L) (L1 and L2) under four ecological conditions of the year–location interaction (E1–E4)
and in two densities (D1 and D2) (50,000 and 65,000 plants ha−1
). Prior to tasselling, the following
five treatments of detasseling and defoliation (T) were applied: T1—control, no leaf removal only
detasseling, T2–T5—removal of tassels and top leaves (from one to four top leaves). The defoliation
treatments had the most pronounced effect on the yield reduction in G1 (T1–Tn+1 . . . T5), p < 0.05.
The ecological conditions on yield variability were expressed under poor weather conditions (E3 and
E4), while lower densities were less favorable for the application of defoliation treatments. The result
of joint effects of factors was the lowest grain yield (896 kg/ha) in G3 in the variant E3D1 for T2 and
the highest grain yield (11,389 kg/ha) in G3 in the variant E2D2 for T1. The smallest effect of the
defoliation treatment was on the kernel row number (KRN)",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Agriculture",
title = "Direct and Joint Effects of Genotype, Defoliation and Crop Density on the Yield of Three Inbred Maize Lines",
volume = "11",
number = "6",
pages = "509",
doi = "10.3390/ agriculture11060509"
}
Ranković, D., Todorović, G., Tabaković, M., Prodanović, S., Boćanski, J.,& Delić, N.. (2021). Direct and Joint Effects of Genotype, Defoliation and Crop Density on the Yield of Three Inbred Maize Lines. in Agriculture
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 11(6), 509.
https://doi.org/10.3390/ agriculture11060509
Ranković D, Todorović G, Tabaković M, Prodanović S, Boćanski J, Delić N. Direct and Joint Effects of Genotype, Defoliation and Crop Density on the Yield of Three Inbred Maize Lines. in Agriculture. 2021;11(6):509.
doi:10.3390/ agriculture11060509 .
Ranković, Dejan, Todorović, Goran, Tabaković, Marijenka, Prodanović, Slaven, Boćanski, Jan, Delić, Nenad, "Direct and Joint Effects of Genotype, Defoliation and Crop Density on the Yield of Three Inbred Maize Lines" in Agriculture, 11, no. 6 (2021):509,
https://doi.org/10.3390/ agriculture11060509 . .

Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia

Nikolić, Milica; Savić, Iva; Nikolić, Ana; Jauković, Marko; Kandić, Vesna; Stevanović, Milan; Stanković, Slavica

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Nikolić, Ana
AU  - Jauković, Marko
AU  - Kandić, Vesna
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/836
AB  - In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Toxins
T1  - Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia
VL  - 13
SP  - 847
DO  - 10.3390/toxins13120847
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Nikolić, Ana and Jauković, Marko and Kandić, Vesna and Stevanović, Milan and Stanković, Slavica",
year = "2021",
abstract = "In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Toxins",
title = "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia",
volume = "13",
pages = "847",
doi = "10.3390/toxins13120847"
}
Nikolić, M., Savić, I., Nikolić, A., Jauković, M., Kandić, V., Stevanović, M.,& Stanković, S.. (2021). Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 13, 847.
https://doi.org/10.3390/toxins13120847
Nikolić M, Savić I, Nikolić A, Jauković M, Kandić V, Stevanović M, Stanković S. Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins. 2021;13:847.
doi:10.3390/toxins13120847 .
Nikolić, Milica, Savić, Iva, Nikolić, Ana, Jauković, Marko, Kandić, Vesna, Stevanović, Milan, Stanković, Slavica, "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia" in Toxins, 13 (2021):847,
https://doi.org/10.3390/toxins13120847 . .

The occurrence of mycotoxins in sweet maize hybrids

Nikolić, Milica; Srdić, Jelena; Savić, Iva; Žilić, Slađana; Stevanović, Milan; Kandić, Vesna; Stanković, Slavica

(Belgrade : Serbian Genetics Society, 2021)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Srdić, Jelena
AU  - Savić, Iva
AU  - Žilić, Slađana
AU  - Stevanović, Milan
AU  - Kandić, Vesna
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/833
AB  - The objective of the present study was to investigate the susceptibility of maize hybrids to the
natural mycotoxins contamination: aflatoxin total (AFLA), deoxynivalenol (DON),
zearalenon (ZEA) and fumonisins (FB). Hybrids were grown during two production years
(2019 and 2020) in two locations. Mycotoxin contamination of maize grains was evaluated in
five sweet maize hybrids. Contamination level of investigated hybrids of first cluster was
mainly by below average values of ZEA (all equal to zero) and DON. Hybrids PK4 (S) 2020,
PK6 (MS) 2020 and PK4 (MS) 2020 had below average values for AFLA, while remaining
treatments of this cluster have mostly elevated values of this mycotoxin. Contamination level
of investigated hybrids of second cluster mostly had increased values of mycotoxins ZEA
and DON and below average values of AFLA. Samples did not contain fumonisins.
Mycotoxin contamination were significantly affected by hybrids and years. We have
established that DON and ZEA levels were influenced by the environmental conditions.
There were no significant effects of location on the level of AFLA in the sweet maize
hybrids. The variation in the properties of mycotoxin content (DON) was significantly
influenced by hybrids, and there was no significance of hybrids x location interaction.
Differences were more expressed for the content of ZEA and AFLA compared to the content
of DON. Hybrid PK1 had the lowest content of DON, while it had the highest content of
ZEA. Mycotoxin analyses showed that in all tested hybrids, levels of AFLA, DON, ZEA and
FBs were below the maximum permissible levels stipulated by the legislation of the
European Union and the Republic of Serbia in maize intended for direct human consumption.
These results confirmed that the susceptibility of hybrids is one of the important risks, in addition to climatic factors, for the appearance of toxigenic fungi and their mycotoxins.
Genotype tolerance is very important as a preventive measure, which indicates that breeders
have to pay attention to it in sweet maize breeding programs.
AB  - Cilj ovog rada bio je da se prouči osteljivost hibrida kukuruza na prirodnu kontaminaciju
mikotoksinima (aflatoksin B1 (AFB1), deoksnivalenol (DON), zearalenol (ZEA) i fumonizini
(FB)). Hibrdi su gajeni tokom dve proizvodne godine (2019. i 2020) u dve lokacije.
Kontaminacija zrna kukuruza mikotoksinima ispitivana je na pet hibrida kukuruza šećerca. Nivo
kontaminacije proučavanih hibrida prvog klastera bio je uglavnom ispod prosečnih vrednosti
ZEA (sve vrednosti su bile nula) i DON. Vrednosti AFLA kod hibrida PK4 (S) 2020, PK6 (MS)
2020 i PK4 (MS) 2020 su bile ispod prosečne vrednosti, dok su vrednosti ovog mikotoksina bile
više od prosečnih vrednosti za ostale tretmane ovog klastera. Nivoi kontaminacije proučavanih
hibrida drugog klastera su uglavnom bili viši za mikotoksine ZEA i DON i niži za AFLA.
Fumnonizni nisu utrvđreni u uzorcima. Na kontaminaciju mikotoksinima značajno su uticali
hibiridi i godine. Utvrđeno je da su uslovi sredine uticali na nivoe DON i ZEA. Lokacija nije
značajno uticala na nivo AFLA kod hibrida kukuruza šećerca. Hibridi su značajno uticali na
variranje sadržaja mikotoksina (DON), dok hibrid × lokacija interakcija nije bila značajna.
Razlike su bile izraženije za sadržaj ZEA i ALFA nego za sadržaj DON. Najniži sadržaj DON
utvrđen je kod hibrida PK1, kod koga je sadržaj ZEA bio najviši. Analize mikotoksina pokazuju
da su nivoi AFLA, DON, ZEA i FB u svim ispitivanim hibridima bili ispod maksimalno
dozvoljenih nivoa koji su propisani zankonima Evropske Unije i Republike Srbije za kukuruz
koji je namenjen za direktnu ljudsku konzumaciju. Ovi rezultati potvrđuju da je osetljivost
hibrida jedan od važnih rizika pored klimatskih faktora za pojavu toksigenih gljiva i njihovih
mikotoksima. Tolerantnost genotipa je veoma važna preventivna mera, na koju oplemenjivači
moraju da obrate pažnju u programima oplemenjivanja kukuruza šećerca.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - The occurrence of mycotoxins in sweet maize hybrids
VL  - 53
IS  - 3
SP  - 1311
EP  - 1320
DO  - 10.2298/GENSR2103311N
ER  - 
@article{
author = "Nikolić, Milica and Srdić, Jelena and Savić, Iva and Žilić, Slađana and Stevanović, Milan and Kandić, Vesna and Stanković, Slavica",
year = "2021",
abstract = "The objective of the present study was to investigate the susceptibility of maize hybrids to the
natural mycotoxins contamination: aflatoxin total (AFLA), deoxynivalenol (DON),
zearalenon (ZEA) and fumonisins (FB). Hybrids were grown during two production years
(2019 and 2020) in two locations. Mycotoxin contamination of maize grains was evaluated in
five sweet maize hybrids. Contamination level of investigated hybrids of first cluster was
mainly by below average values of ZEA (all equal to zero) and DON. Hybrids PK4 (S) 2020,
PK6 (MS) 2020 and PK4 (MS) 2020 had below average values for AFLA, while remaining
treatments of this cluster have mostly elevated values of this mycotoxin. Contamination level
of investigated hybrids of second cluster mostly had increased values of mycotoxins ZEA
and DON and below average values of AFLA. Samples did not contain fumonisins.
Mycotoxin contamination were significantly affected by hybrids and years. We have
established that DON and ZEA levels were influenced by the environmental conditions.
There were no significant effects of location on the level of AFLA in the sweet maize
hybrids. The variation in the properties of mycotoxin content (DON) was significantly
influenced by hybrids, and there was no significance of hybrids x location interaction.
Differences were more expressed for the content of ZEA and AFLA compared to the content
of DON. Hybrid PK1 had the lowest content of DON, while it had the highest content of
ZEA. Mycotoxin analyses showed that in all tested hybrids, levels of AFLA, DON, ZEA and
FBs were below the maximum permissible levels stipulated by the legislation of the
European Union and the Republic of Serbia in maize intended for direct human consumption.
These results confirmed that the susceptibility of hybrids is one of the important risks, in addition to climatic factors, for the appearance of toxigenic fungi and their mycotoxins.
Genotype tolerance is very important as a preventive measure, which indicates that breeders
have to pay attention to it in sweet maize breeding programs., Cilj ovog rada bio je da se prouči osteljivost hibrida kukuruza na prirodnu kontaminaciju
mikotoksinima (aflatoksin B1 (AFB1), deoksnivalenol (DON), zearalenol (ZEA) i fumonizini
(FB)). Hibrdi su gajeni tokom dve proizvodne godine (2019. i 2020) u dve lokacije.
Kontaminacija zrna kukuruza mikotoksinima ispitivana je na pet hibrida kukuruza šećerca. Nivo
kontaminacije proučavanih hibrida prvog klastera bio je uglavnom ispod prosečnih vrednosti
ZEA (sve vrednosti su bile nula) i DON. Vrednosti AFLA kod hibrida PK4 (S) 2020, PK6 (MS)
2020 i PK4 (MS) 2020 su bile ispod prosečne vrednosti, dok su vrednosti ovog mikotoksina bile
više od prosečnih vrednosti za ostale tretmane ovog klastera. Nivoi kontaminacije proučavanih
hibrida drugog klastera su uglavnom bili viši za mikotoksine ZEA i DON i niži za AFLA.
Fumnonizni nisu utrvđreni u uzorcima. Na kontaminaciju mikotoksinima značajno su uticali
hibiridi i godine. Utvrđeno je da su uslovi sredine uticali na nivoe DON i ZEA. Lokacija nije
značajno uticala na nivo AFLA kod hibrida kukuruza šećerca. Hibridi su značajno uticali na
variranje sadržaja mikotoksina (DON), dok hibrid × lokacija interakcija nije bila značajna.
Razlike su bile izraženije za sadržaj ZEA i ALFA nego za sadržaj DON. Najniži sadržaj DON
utvrđen je kod hibrida PK1, kod koga je sadržaj ZEA bio najviši. Analize mikotoksina pokazuju
da su nivoi AFLA, DON, ZEA i FB u svim ispitivanim hibridima bili ispod maksimalno
dozvoljenih nivoa koji su propisani zankonima Evropske Unije i Republike Srbije za kukuruz
koji je namenjen za direktnu ljudsku konzumaciju. Ovi rezultati potvrđuju da je osetljivost
hibrida jedan od važnih rizika pored klimatskih faktora za pojavu toksigenih gljiva i njihovih
mikotoksima. Tolerantnost genotipa je veoma važna preventivna mera, na koju oplemenjivači
moraju da obrate pažnju u programima oplemenjivanja kukuruza šećerca.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "The occurrence of mycotoxins in sweet maize hybrids",
volume = "53",
number = "3",
pages = "1311-1320",
doi = "10.2298/GENSR2103311N"
}
Nikolić, M., Srdić, J., Savić, I., Žilić, S., Stevanović, M., Kandić, V.,& Stanković, S.. (2021). The occurrence of mycotoxins in sweet maize hybrids. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1311-1320.
https://doi.org/10.2298/GENSR2103311N
Nikolić M, Srdić J, Savić I, Žilić S, Stevanović M, Kandić V, Stanković S. The occurrence of mycotoxins in sweet maize hybrids. in Genetika. 2021;53(3):1311-1320.
doi:10.2298/GENSR2103311N .
Nikolić, Milica, Srdić, Jelena, Savić, Iva, Žilić, Slađana, Stevanović, Milan, Kandić, Vesna, Stanković, Slavica, "The occurrence of mycotoxins in sweet maize hybrids" in Genetika, 53, no. 3 (2021):1311-1320,
https://doi.org/10.2298/GENSR2103311N . .

The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain

Savić, Iva; Nikolić, Milica; Vico, Ivana; Mladenović-Drinić, Snežana; Duduk, Natasa; Stanković, Slavica

(Beograd : Društvo genetičara Srbija, 2021)

TY  - JOUR
AU  - Savić, Iva
AU  - Nikolić, Milica
AU  - Vico, Ivana
AU  - Mladenović-Drinić, Snežana
AU  - Duduk, Natasa
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/830
AB  - During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.
AB  - Tokom perioda od 2014. do 2017. godine sakupljani su uzorci pšenice sa fuzarioznih klasova sa 20 lokacija u Srbiji. Nakon izolacije, gljive su gajene na KDA podlozi 7 dana u termostatu na temperaturi od 25oC. Na osnovu identifikacije in situ na KDA (krompir dekstrozni agar), 36 izolata sekcije Liseola odabrano je za dalje analize. Potvrđena je patogenost svih izolata na listovima pšenice. Virulentnost izolata utvrđena je merenjem dužina formirane pege na mestu inokulacije. Za dokazivanje prisustva vrste Fusarium verticillioides korišćen je par prajmera FV-F2/FV-R koji umnožava sekvence gaoB gena i koji se pokazao kao specifičan za navedenu vrstu. Takođe, sa istim ciljem korišćen je par prajmera VER1-VER2 dizajniran na osnovu dela genske sekvence za kalmodulin. Kao pozitivna kontrola korišćen je referentni izolat RBG 1603 Q27. Par prajmera VER1-VER2 obrazovao je traku očekivane veličine (578bp) kod 18 izolata, dok je pomoću para prajmera FV-F2/FV-R traka dužine 370bp potvrdila prisustvo F. verticillioides kod 16 izolata. Šesnaest od 18 izolata koji su identifikovani VER1-VER2 parom, takođe su identifikovani i FV-FS/FV-R prajmerima. U negativnoj kontroli nije bilo amplifikacije.
PB  - Beograd : Društvo genetičara Srbija
T2  - Genetika
T1  - The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain
VL  - 53
IS  - 2
SP  - 641
EP  - 649
DO  - 10.2298/GENSR2102641S
ER  - 
@article{
author = "Savić, Iva and Nikolić, Milica and Vico, Ivana and Mladenović-Drinić, Snežana and Duduk, Natasa and Stanković, Slavica",
year = "2021",
abstract = "During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control., Tokom perioda od 2014. do 2017. godine sakupljani su uzorci pšenice sa fuzarioznih klasova sa 20 lokacija u Srbiji. Nakon izolacije, gljive su gajene na KDA podlozi 7 dana u termostatu na temperaturi od 25oC. Na osnovu identifikacije in situ na KDA (krompir dekstrozni agar), 36 izolata sekcije Liseola odabrano je za dalje analize. Potvrđena je patogenost svih izolata na listovima pšenice. Virulentnost izolata utvrđena je merenjem dužina formirane pege na mestu inokulacije. Za dokazivanje prisustva vrste Fusarium verticillioides korišćen je par prajmera FV-F2/FV-R koji umnožava sekvence gaoB gena i koji se pokazao kao specifičan za navedenu vrstu. Takođe, sa istim ciljem korišćen je par prajmera VER1-VER2 dizajniran na osnovu dela genske sekvence za kalmodulin. Kao pozitivna kontrola korišćen je referentni izolat RBG 1603 Q27. Par prajmera VER1-VER2 obrazovao je traku očekivane veličine (578bp) kod 18 izolata, dok je pomoću para prajmera FV-F2/FV-R traka dužine 370bp potvrdila prisustvo F. verticillioides kod 16 izolata. Šesnaest od 18 izolata koji su identifikovani VER1-VER2 parom, takođe su identifikovani i FV-FS/FV-R prajmerima. U negativnoj kontroli nije bilo amplifikacije.",
publisher = "Beograd : Društvo genetičara Srbija",
journal = "Genetika",
title = "The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain",
volume = "53",
number = "2",
pages = "641-649",
doi = "10.2298/GENSR2102641S"
}
Savić, I., Nikolić, M., Vico, I., Mladenović-Drinić, S., Duduk, N.,& Stanković, S.. (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. in Genetika
Beograd : Društvo genetičara Srbija., 53(2), 641-649.
https://doi.org/10.2298/GENSR2102641S
Savić I, Nikolić M, Vico I, Mladenović-Drinić S, Duduk N, Stanković S. The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. in Genetika. 2021;53(2):641-649.
doi:10.2298/GENSR2102641S .
Savić, Iva, Nikolić, Milica, Vico, Ivana, Mladenović-Drinić, Snežana, Duduk, Natasa, Stanković, Slavica, "The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain" in Genetika, 53, no. 2 (2021):641-649,
https://doi.org/10.2298/GENSR2102641S . .

Application of standard germination and vigour tests for seed quality assessment of maize inbred lines

Milivojević, Marija; Srdić, Jelena; Filipović, Milomir; Petrović, Tanja; Branković-Radojčić, Dragana V.; Marković, Ksenija; Boćanski, Jan

(Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije, 2021)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Filipović, Milomir
AU  - Petrović, Tanja
AU  - Branković-Radojčić, Dragana V.
AU  - Marković, Ksenija
AU  - Boćanski, Jan
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/924
AB  - Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field 
conditions and seed longevity is essential for seed companies. The objective of this work was to 
evaluate different vigour tests for detecting differences among naturally aged seeds and to assess 
the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed 
of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different 
heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings 
was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines 
was detected in seed vigour determined by three tests, in particular by the accelerated ageing test.
Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines 
with Lancaster background. The correlation between the cold test and the accelerated ageing test 
was significant, while the radical emergence test had a poor correlation with them. Obtained 
results indicate that the application of either cold test or accelerated ageing test is reliable for 
screening seed vigour of maize inbred lines.
AB  - Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod-
nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog
rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog
semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena
i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza
selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim
grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom
standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad-
nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana
Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve
parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu
vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad-
nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom
nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa
ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa
njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana
za skrining vigora semena samooplodnih linija kukuruza
PB  - Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Application of standard germination and vigour tests for seed quality assessment of maize inbred lines
T1  - Primena testova standardne klijavosti i vigor testova u proceni kvaliteta semena samooplodnih linija kukuruza
VL  - 27
IS  - 2
SP  - 35
EP  - 45
DO  - 10.5937/SelSem2102035M
ER  - 
@article{
author = "Milivojević, Marija and Srdić, Jelena and Filipović, Milomir and Petrović, Tanja and Branković-Radojčić, Dragana V. and Marković, Ksenija and Boćanski, Jan",
year = "2021",
abstract = "Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field 
conditions and seed longevity is essential for seed companies. The objective of this work was to 
evaluate different vigour tests for detecting differences among naturally aged seeds and to assess 
the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed 
of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different 
heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings 
was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines 
was detected in seed vigour determined by three tests, in particular by the accelerated ageing test.
Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines 
with Lancaster background. The correlation between the cold test and the accelerated ageing test 
was significant, while the radical emergence test had a poor correlation with them. Obtained 
results indicate that the application of either cold test or accelerated ageing test is reliable for 
screening seed vigour of maize inbred lines., Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod-
nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog
rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog
semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena
i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza
selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim
grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom
standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad-
nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana
Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve
parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu
vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad-
nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom
nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa
ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa
njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana
za skrining vigora semena samooplodnih linija kukuruza",
publisher = "Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Application of standard germination and vigour tests for seed quality assessment of maize inbred lines, Primena testova standardne klijavosti i vigor testova u proceni kvaliteta semena samooplodnih linija kukuruza",
volume = "27",
number = "2",
pages = "35-45",
doi = "10.5937/SelSem2102035M"
}
Milivojević, M., Srdić, J., Filipović, M., Petrović, T., Branković-Radojčić, D. V., Marković, K.,& Boćanski, J.. (2021). Application of standard germination and vigour tests for seed quality assessment of maize inbred lines. in Selekcija i semenarstvo
Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije., 27(2), 35-45.
https://doi.org/10.5937/SelSem2102035M
Milivojević M, Srdić J, Filipović M, Petrović T, Branković-Radojčić DV, Marković K, Boćanski J. Application of standard germination and vigour tests for seed quality assessment of maize inbred lines. in Selekcija i semenarstvo. 2021;27(2):35-45.
doi:10.5937/SelSem2102035M .
Milivojević, Marija, Srdić, Jelena, Filipović, Milomir, Petrović, Tanja, Branković-Radojčić, Dragana V., Marković, Ksenija, Boćanski, Jan, "Application of standard germination and vigour tests for seed quality assessment of maize inbred lines" in Selekcija i semenarstvo, 27, no. 2 (2021):35-45,
https://doi.org/10.5937/SelSem2102035M . .

Quality parameterd and potentials of utilization of different maize hybrids for food and feed

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Radosavljević, Milica; Filipović, Milomir; Srdić, Jelena

(Cacak : University of Kragujevac, Faculty of Agronomy, 2021)

TY  - CONF
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Radosavljević, Milica
AU  - Filipović, Milomir
AU  - Srdić, Jelena
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/876
AB  - Quality parameters of six maize hybrids created at the Maize
Research Institute Zemun Polje were investigated in this study. Physical
properties, kernel structure, and chemical composition of one yellow dent
standard and five specialty maize hybrids of different grain color were analyzed.
Whole-grain maize flour is naturally gluten-free which makes it suitable for
persons suffering from celiac disease. Fiber, protein, and oil make maize grain an
essential component for animal feed production. All maize hybrids showed
favorable processing and nutritive characteristics which make them highly suitable
for different uses.
PB  - Cacak : University of Kragujevac, Faculty of Agronomy
C3  - 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova
T1  - Quality parameterd and potentials of utilization of different maize hybrids for food and feed
SP  - 495
EP  - 500
DO  - 10.46793/SBT26.495N
ER  - 
@conference{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Radosavljević, Milica and Filipović, Milomir and Srdić, Jelena",
year = "2021",
abstract = "Quality parameters of six maize hybrids created at the Maize
Research Institute Zemun Polje were investigated in this study. Physical
properties, kernel structure, and chemical composition of one yellow dent
standard and five specialty maize hybrids of different grain color were analyzed.
Whole-grain maize flour is naturally gluten-free which makes it suitable for
persons suffering from celiac disease. Fiber, protein, and oil make maize grain an
essential component for animal feed production. All maize hybrids showed
favorable processing and nutritive characteristics which make them highly suitable
for different uses.",
publisher = "Cacak : University of Kragujevac, Faculty of Agronomy",
journal = "26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova",
title = "Quality parameterd and potentials of utilization of different maize hybrids for food and feed",
pages = "495-500",
doi = "10.46793/SBT26.495N"
}
Nikolić, V., Žilić, S., Simić, M., Radosavljević, M., Filipović, M.,& Srdić, J.. (2021). Quality parameterd and potentials of utilization of different maize hybrids for food and feed. in 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova
Cacak : University of Kragujevac, Faculty of Agronomy., 495-500.
https://doi.org/10.46793/SBT26.495N
Nikolić V, Žilić S, Simić M, Radosavljević M, Filipović M, Srdić J. Quality parameterd and potentials of utilization of different maize hybrids for food and feed. in 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova. 2021;:495-500.
doi:10.46793/SBT26.495N .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Radosavljević, Milica, Filipović, Milomir, Srdić, Jelena, "Quality parameterd and potentials of utilization of different maize hybrids for food and feed" in 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova (2021):495-500,
https://doi.org/10.46793/SBT26.495N . .

Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit

Kravić, Natalija; Babić, Vojka; Vukadinović, Jelena; Ristić, Danijela; Dragičević, Vesna; Mladenović-Drinić, Snežana; Anđelković, Violeta

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Vukadinović, Jelena
AU  - Ristić, Danijela
AU  - Dragičević, Vesna
AU  - Mladenović-Drinić, Snežana
AU  - Anđelković, Violeta
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/828
AB  - Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biology
T1  - Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit
VL  - 10
IS  - 8
SP  - 694
DO  - 10.3390/biology10080694
ER  - 
@article{
author = "Kravić, Natalija and Babić, Vojka and Vukadinović, Jelena and Ristić, Danijela and Dragičević, Vesna and Mladenović-Drinić, Snežana and Anđelković, Violeta",
year = "2021",
abstract = "Plants reconfigure their metabolic pathways to cope with water deficit. The aim of this study was to determine the status of the physiological parameters and the content of phenolic acids in the upper most ear leaf of maize inbred lines contrasting in drought tolerance in terms of improved plant productivity e.g., increased grain yield. The experiment was conducted under irrigation and rain-fed conditions. In drought-tolerant lines, the effect of water deficit was reflected through a chlorophyll and nitrogen balance index increase followed by a flavonols index decrease. The opposite trend was noticed in drought susceptible inbreds, with the exception of the anthocyanins index. Moreover, in comparison to irrigation treatment, opposite trends in the correlations between grain yield and physiological parameters found under water deficit conditions indicated the activation of different metabolic pathways in defense against water deficit stress. Concerning phenolic acid content, water deficit caused the reduction of protocatechuic, caffeic, and sinapic acid in all inbreds evaluated. However, the highly pronounced increase of ferulic and especially cinnamic acid content under water deficit conditions indicated possible crucial role of these secondary metabolites in preventing the harmful effects of water deficit stress, which, in turn, might be useful in maize breeding selection for drought tolerance",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biology",
title = "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit",
volume = "10",
number = "8",
pages = "694",
doi = "10.3390/biology10080694"
}
Kravić, N., Babić, V., Vukadinović, J., Ristić, D., Dragičević, V., Mladenović-Drinić, S.,& Anđelković, V.. (2021). Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 694.
https://doi.org/10.3390/biology10080694
Kravić N, Babić V, Vukadinović J, Ristić D, Dragičević V, Mladenović-Drinić S, Anđelković V. Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit. in Biology. 2021;10(8):694.
doi:10.3390/biology10080694 .
Kravić, Natalija, Babić, Vojka, Vukadinović, Jelena, Ristić, Danijela, Dragičević, Vesna, Mladenović-Drinić, Snežana, Anđelković, Violeta, "Alteration of metabolites accumulation in maize inbreds leaf tissue under long-term water deficit" in Biology, 10, no. 8 (2021):694,
https://doi.org/10.3390/biology10080694 . .
2
2