Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods

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Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods (en)
Редукција токсигених гљива рода Фусариум и њихових микотоксина у производњи здравствено безбедне хране на бази жита (sr)
Redukcija toksigenih gljiva roda Fusarium i njihovih mikotoksina u proizvodnji zdravstveno bezbedne hrane na bazi žita (sr_RS)
Authors

Publications

First report of Aspergillus parasiticus on Barley Grain in Serbia

Nikolić, Milica; Savić, Iva; Obradović, Ana; Srdić, Jelena; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : The American Phytopathological Society (APS), 2020)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Obradović, Ana
AU  - Srdić, Jelena
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/796
AB  - Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.
PB  - St. Paul : The American Phytopathological Society (APS)
T2  - Plant Disease
T1  - First report of Aspergillus parasiticus on Barley Grain in Serbia
VL  - 104
IS  - 3
SP  - 987
DO  - 10.1094/PDIS-07-19-1364-PDN
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Obradović, Ana and Srdić, Jelena and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2020",
url = "http://rik.mrizp.rs/handle/123456789/796",
abstract = "Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.",
publisher = "St. Paul : The American Phytopathological Society (APS)",
journal = "Plant Disease",
title = "First report of Aspergillus parasiticus on Barley Grain in Serbia",
volume = "104",
number = "3",
pages = "987",
doi = "10.1094/PDIS-07-19-1364-PDN"
}
Nikolić, M., Savić, I., Obradović, A., Srdić, J., Stanković, G., Stevanović, M.,& Stanković, S. (2020). First report of Aspergillus parasiticus on Barley Grain in Serbia.
Plant Disease
St. Paul : The American Phytopathological Society (APS)., 104(3), 987.
https://doi.org/10.1094/PDIS-07-19-1364-PDN
Nikolić M, Savić I, Obradović A, Srdić J, Stanković G, Stevanović M, Stanković S. First report of Aspergillus parasiticus on Barley Grain in Serbia. Plant Disease. 2020;104(3):987
Nikolić Milica, Savić Iva, Obradović Ana, Srdić Jelena, Stanković Goran, Stevanović Milan, Stanković Slavica, "First report of Aspergillus parasiticus on Barley Grain in Serbia" Plant Disease, 104, no. 3 (2020):987,
https://doi.org/10.1094/PDIS-07-19-1364-PDN .

Aspergillus parasiticus Speare – toksigena vrsta gljive na zrnu kukuruza u proizvodnim uslovima Srbije

Nikolić, Milica

(2019)

TY  - BOOK
AU  - Nikolić, Milica
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/771
AB  - In the course of four-year studies (2013-2016), the species Aspergillus parasiticus was identified in Serbia for the first time as a causal agent of aspergillus kernel and ear rot in both fields and storages. In order to examine the distribution and diversity of this species, the study of morphological, pathogenic, molecular and toxigenic properties of 48 isolates of this species selected from maize kernels was carried out. Studies of macroscopic morphological traits of isolates showed that biodiversity of observed isolates was low. Colonies of the isolates formed on PDA the abundant dark green, dense cottony mycelium coating. All isolates of A. parasiticus formed unicellular denticulate conidia on MEA. The diameter of conidia of observed isolates varied from 4.08μm to 6.86μm.Two factorial analysis of variance showed that the effects of interactions of studied isolates, light regime and temperatures (p<0.01) on daily growth of the colony diameters were statistically significant. The pathogenicity test pointed out that all observed isolates of A. parasiticus were pathogens of maize ears, as well as that there was divergence in the virulence expression.The comparison of concentrations of synthesised mycotoxins in isolates obtained by ELISA showed a statistically highly significant positive correlation between potential to produce AFB1 and AFLA in tested isolates (r=0,66 μm).The observed isolates A. parasiticus that synthesised aflatoxins (50%) mostly synthesised AFB1 (84.78%), while the number of isolates that synthesised AFG1 was significantly lower (15.22%). However, it was established that eight isolates synthesised AFG1 at higher concentrations than AFB1. Mycotoxin concentrations in A. parasiticusisolates were compared by using the results obtained by the HPLC method. The comparison indicated that there was a highly statistically positive correlation between synthesised concentrations of AFB1 and AFG1 (r=0.82 μm), as well as between AFB2 and AFG2 (r=0.63 μm).The identity of all isolates previously characterised on the basis of morphological, ecological and toxigenic properties as species A. parasiticus was confirmed by the multiplex PCR detection method. Distinguishing between species A. parasiticus and morphologically similar species A. flavus was enabled by the application of the RFLP-PCR method, using restriction enzymes. This was also the first identification of this pathogenic species at the molecular level in Serbia.
AB  - U toku četvorogodišnjih ispitivanja (2013-2016) identifikovana je, po prvi put u Srbiji, vrsta Asperigillus parasiticus kao prouzrokovač aspergilozne truleži zrna i klipa kukuruza u polju i skladištima. Sa ciljem da se ispita rasprostranjenost i diverzitet ove vrste obavljena su proučavanja morfoloških, patogenih, molekularnih, odgajivačkih i toksigenih svojstava 46 odabranih izolata ove vrste poreklom sa zrna kukuruza. Ispitivanjem makroskopskih morfoloških karakteristika izolata ukazano je na mali biodiverzitet proučavanih izolata. Kolonije izolata na PDA hranljivoj podlozi formirale su obilnu tamnozelenu, gustu prevlaku od micelije gljive, pamučastog izgleda. Svi izolati A. parasiticus obrazuju jednoćelijske, nazubljene konidije na MEA hranljivoj podlozi. Prečnik konidija ispitivanih izolata varirao je od 4,08μm do 6,86μm. Dvofaktorska analiza varijanse pokazala je statistički značajan uticaj interakcije ispitivanih izolata, svetlosnog režima i temperature (p<0.01) na dnevni porast prečnika kolonija. Test patogenosti ukazao je da su svi ispitivani izolati A. parasiticus patogeni na klipu kukuruza, kao i da postoji divergentnost u ispoljavanju virulentnosti. Poređenjem koncentracija sintetisanih mikotoksina kod izolata dobijenih Elisa testom, pokazali su da postoji statistički visoko značajna pozitivna korelacija između potencijala produkcije AFB1 i AFLA kod ispitivanih izolata (r=0,66 μm). Ispitivani izolati A.parasiticus koji su sintetisali aflatoksine (50%) su u najvećem procentu sintetisali AFB1 (84,78%), dok je procenat izolata koji su sintetisali AFG1 bila znatno manja (15,22%). Međutim, utvrđeno je osam izolata koji su sintetisali AFG1 u višim koncentracijama, u odnosu na AFB1. Poređenjem koncentracija mikotoksina kod izolata A. parasiticus, na osnovu rezultata HPLC metode, uočeno je da postoji visoko statistički značajna pozitivna korelacija između sintetisanih koncentracija AFB1 i AFG1 (r=0,82 μm), kao i između AFB2 i AFG2 (r=0,63 μm). Multiplex PCR detekcijom potvrđen je identitet svih izolata prethodno okarakterisanih na osnovu morfoloških, ekoloških, odgajivačkih i toksigenih karakteristika kao vrsta A. parasiticus. Primenom RFLP-PCR metode korišćenjem restrikcionih enzima, omogućeno je razlikovanje vrste A. parasiticus od morfološki slične vrste A. flavus, što je ujedno i prva identifikacija ove patogene vrste na molekularnom nivou u Srbiji.
T1  - Aspergillus parasiticus Speare – toksigena vrsta gljive na zrnu kukuruza u proizvodnim uslovima Srbije
T1  - Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia
ER  - 
@book{
author = "Nikolić, Milica",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/771",
abstract = "In the course of four-year studies (2013-2016), the species Aspergillus parasiticus was identified in Serbia for the first time as a causal agent of aspergillus kernel and ear rot in both fields and storages. In order to examine the distribution and diversity of this species, the study of morphological, pathogenic, molecular and toxigenic properties of 48 isolates of this species selected from maize kernels was carried out. Studies of macroscopic morphological traits of isolates showed that biodiversity of observed isolates was low. Colonies of the isolates formed on PDA the abundant dark green, dense cottony mycelium coating. All isolates of A. parasiticus formed unicellular denticulate conidia on MEA. The diameter of conidia of observed isolates varied from 4.08μm to 6.86μm.Two factorial analysis of variance showed that the effects of interactions of studied isolates, light regime and temperatures (p<0.01) on daily growth of the colony diameters were statistically significant. The pathogenicity test pointed out that all observed isolates of A. parasiticus were pathogens of maize ears, as well as that there was divergence in the virulence expression.The comparison of concentrations of synthesised mycotoxins in isolates obtained by ELISA showed a statistically highly significant positive correlation between potential to produce AFB1 and AFLA in tested isolates (r=0,66 μm).The observed isolates A. parasiticus that synthesised aflatoxins (50%) mostly synthesised AFB1 (84.78%), while the number of isolates that synthesised AFG1 was significantly lower (15.22%). However, it was established that eight isolates synthesised AFG1 at higher concentrations than AFB1. Mycotoxin concentrations in A. parasiticusisolates were compared by using the results obtained by the HPLC method. The comparison indicated that there was a highly statistically positive correlation between synthesised concentrations of AFB1 and AFG1 (r=0.82 μm), as well as between AFB2 and AFG2 (r=0.63 μm).The identity of all isolates previously characterised on the basis of morphological, ecological and toxigenic properties as species A. parasiticus was confirmed by the multiplex PCR detection method. Distinguishing between species A. parasiticus and morphologically similar species A. flavus was enabled by the application of the RFLP-PCR method, using restriction enzymes. This was also the first identification of this pathogenic species at the molecular level in Serbia., U toku četvorogodišnjih ispitivanja (2013-2016) identifikovana je, po prvi put u Srbiji, vrsta Asperigillus parasiticus kao prouzrokovač aspergilozne truleži zrna i klipa kukuruza u polju i skladištima. Sa ciljem da se ispita rasprostranjenost i diverzitet ove vrste obavljena su proučavanja morfoloških, patogenih, molekularnih, odgajivačkih i toksigenih svojstava 46 odabranih izolata ove vrste poreklom sa zrna kukuruza. Ispitivanjem makroskopskih morfoloških karakteristika izolata ukazano je na mali biodiverzitet proučavanih izolata. Kolonije izolata na PDA hranljivoj podlozi formirale su obilnu tamnozelenu, gustu prevlaku od micelije gljive, pamučastog izgleda. Svi izolati A. parasiticus obrazuju jednoćelijske, nazubljene konidije na MEA hranljivoj podlozi. Prečnik konidija ispitivanih izolata varirao je od 4,08μm do 6,86μm. Dvofaktorska analiza varijanse pokazala je statistički značajan uticaj interakcije ispitivanih izolata, svetlosnog režima i temperature (p<0.01) na dnevni porast prečnika kolonija. Test patogenosti ukazao je da su svi ispitivani izolati A. parasiticus patogeni na klipu kukuruza, kao i da postoji divergentnost u ispoljavanju virulentnosti. Poređenjem koncentracija sintetisanih mikotoksina kod izolata dobijenih Elisa testom, pokazali su da postoji statistički visoko značajna pozitivna korelacija između potencijala produkcije AFB1 i AFLA kod ispitivanih izolata (r=0,66 μm). Ispitivani izolati A.parasiticus koji su sintetisali aflatoksine (50%) su u najvećem procentu sintetisali AFB1 (84,78%), dok je procenat izolata koji su sintetisali AFG1 bila znatno manja (15,22%). Međutim, utvrđeno je osam izolata koji su sintetisali AFG1 u višim koncentracijama, u odnosu na AFB1. Poređenjem koncentracija mikotoksina kod izolata A. parasiticus, na osnovu rezultata HPLC metode, uočeno je da postoji visoko statistički značajna pozitivna korelacija između sintetisanih koncentracija AFB1 i AFG1 (r=0,82 μm), kao i između AFB2 i AFG2 (r=0,63 μm). Multiplex PCR detekcijom potvrđen je identitet svih izolata prethodno okarakterisanih na osnovu morfoloških, ekoloških, odgajivačkih i toksigenih karakteristika kao vrsta A. parasiticus. Primenom RFLP-PCR metode korišćenjem restrikcionih enzima, omogućeno je razlikovanje vrste A. parasiticus od morfološki slične vrste A. flavus, što je ujedno i prva identifikacija ove patogene vrste na molekularnom nivou u Srbiji.",
title = "Aspergillus parasiticus Speare – toksigena vrsta gljive na zrnu kukuruza u proizvodnim uslovima Srbije, Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia"
}
Nikolić, M. (2019). Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia.
.
Nikolić M. Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia. 2019;
Nikolić Milica, "Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia" (2019)

Aspergillus parasiticus Speare – toksigena vrsta gljive na zrnu kukuruza u proizvodnim uslovima Srbije

Nikolić, Milica

(Novi Sad, Univerzitet u Novom Sadu, Poljoprivredni fakultet, 2019)

TY  - BOOK
AU  - Nikolić, Milica
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/770
AB  - In the course of four-year studies (2013-2016), the species Aspergillus parasiticus was identified in Serbia for the first time as a causal agent of aspergillus kernel and ear rot in both fields and storages. In order to examine the distribution and diversity of this species, the study of morphological, pathogenic, molecular and toxigenic properties of 48 isolates of this species selected from maize kernels was carried out. Studies of macroscopic morphological traits of isolates showed that biodiversity of observed isolates was low. Colonies of the isolates formed on PDA the abundant dark green, dense cottony mycelium coating. All isolates of A. parasiticus formed unicellular denticulate conidia on MEA. The diameter of conidia of observed isolates varied from 4.08μm to 6.86μm.
Two factorial analysis of variance showed that the effects of interactions of studied isolates, light regime and temperatures (p<0.01) on daily growth of the colony diameters were statistically significant. The pathogenicity test pointed out that all observed isolates of A. parasiticus were pathogens of maize ears, as well as that there was divergence in the virulence expression.The comparison of concentrations of synthesised mycotoxins in isolates obtained by ELISA showed a statistically highly significant positive correlation between potential to produce AFB1 and AFLA in tested isolates (r=0,66 μm).The observed isolates A. parasiticus that synthesised aflatoxins (50%) mostly synthesised AFB1 (84.78%), while the number of isolates that synthesised AFG1 was significantly lower (15.22%). However, it was established that eight isolates synthesised AFG1 at higher concentrations than AFB1. Mycotoxin concentrations in A. parasiticusisolates were compared by using the results obtained by the HPLC method. The comparison indicated that there was a highly statistically positive correlation between synthesised concentrations of AFB1 and AFG1 (r=0.82 μm), as well as between AFB2 and AFG2 (r=0.63 μm).The identity of all isolates previously characterised on the basis of morphological, ecological and toxigenic properties as species A. parasiticus was confirmed by the multiplex PCR detection method. Distinguishing between species A. parasiticus and morphologically similar species A. flavus was enabled by the application of the RFLP-PCR method, using restriction enzymes. This was also the first identification of this pathogenic species at the molecular level in Serbia.
AB  - U toku četvorogodišnjih ispitivanja (2013-2016) identifikovana je, po prvi put u Srbiji, vrsta Asperigillus parasiticus kao prouzrokovač aspergilozne truleži zrna i klipa kukuruza u polju i skladištima. Sa ciljem da se ispita rasprostranjenost i diverzitet ove vrste obavljena su proučavanja morfoloških, patogenih, molekularnih, odgajivačkih i toksigenih svojstava 46 odabranih izolata ove vrste poreklom sa zrna kukuruza. Ispitivanjem makroskopskih morfoloških karakteristika izolata ukazano je na mali biodiverzitet proučavanih izolata. Kolonije izolata na PDA hranljivoj podlozi formirale su obilnu tamnozelenu, gustu prevlaku od micelije gljive, pamučastog izgleda. Svi izolati A. parasiticus obrazuju jednoćelijske, nazubljene konidije na MEA hranljivoj podlozi. Prečnik konidija ispitivanih izolata varirao je od 4,08μm do 6,86μm. Dvofaktorska analiza varijanse pokazala je statistički značajan uticaj interakcije ispitivanih izolata, svetlosnog režima i temperature (p<0.01) na dnevni porast prečnika kolonija. Test patogenosti ukazao je da su svi ispitivani izolati A. parasiticus patogeni na klipu kukuruza, kao i da postoji divergentnost u ispoljavanju virulentnosti. Poređenjem koncentracija sintetisanih mikotoksina kod izolata dobijenih Elisa testom, pokazali su da postoji statistički visoko značajna pozitivna korelacija između potencijala produkcije AFB1 i AFLA kod ispitivanih izolata (r=0,66 μm). Ispitivani izolati A.parasiticus koji su sintetisali aflatoksine (50%) su u najvećem procentu sintetisali AFB1 (84,78%), dok je procenat izolata koji su sintetisali AFG1 bila znatno manja (15,22%). Međutim, utvrđeno je osam izolata koji su sintetisali AFG1 u višim koncentracijama, u odnosu na AFB1. Poređenjem koncentracija mikotoksina kod izolata A. parasiticus, na osnovu rezultata HPLC metode, uočeno je da postoji visoko statistički značajna pozitivna korelacija između sintetisanih koncentracija AFB1 i AFG1 (r=0,82 μm), kao i između AFB2 i AFG2 (r=0,63 μm). Multiplex PCR detekcijom potvrđen je identitet svih izolata prethodno okarakterisanih na osnovu morfoloških, ekoloških, odgajivačkih i toksigenih karakteristika kao vrsta A. parasiticus. Primenom RFLP-PCR metode korišćenjem restrikcionih enzima, omogućeno je razlikovanje vrste A. parasiticus od morfološki slične vrste A. flavus, što je ujedno i prva identifikacija ove patogene vrste na molekularnom nivou u Srbiji.
PB  - Novi Sad, Univerzitet u Novom Sadu, Poljoprivredni fakultet
T1  - Aspergillus parasiticus Speare – toksigena vrsta gljive na zrnu kukuruza u proizvodnim uslovima Srbije
T1  - Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia
ER  - 
@phdthesis{
author = "Nikolić, Milica",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/770",
abstract = "In the course of four-year studies (2013-2016), the species Aspergillus parasiticus was identified in Serbia for the first time as a causal agent of aspergillus kernel and ear rot in both fields and storages. In order to examine the distribution and diversity of this species, the study of morphological, pathogenic, molecular and toxigenic properties of 48 isolates of this species selected from maize kernels was carried out. Studies of macroscopic morphological traits of isolates showed that biodiversity of observed isolates was low. Colonies of the isolates formed on PDA the abundant dark green, dense cottony mycelium coating. All isolates of A. parasiticus formed unicellular denticulate conidia on MEA. The diameter of conidia of observed isolates varied from 4.08μm to 6.86μm.
Two factorial analysis of variance showed that the effects of interactions of studied isolates, light regime and temperatures (p<0.01) on daily growth of the colony diameters were statistically significant. The pathogenicity test pointed out that all observed isolates of A. parasiticus were pathogens of maize ears, as well as that there was divergence in the virulence expression.The comparison of concentrations of synthesised mycotoxins in isolates obtained by ELISA showed a statistically highly significant positive correlation between potential to produce AFB1 and AFLA in tested isolates (r=0,66 μm).The observed isolates A. parasiticus that synthesised aflatoxins (50%) mostly synthesised AFB1 (84.78%), while the number of isolates that synthesised AFG1 was significantly lower (15.22%). However, it was established that eight isolates synthesised AFG1 at higher concentrations than AFB1. Mycotoxin concentrations in A. parasiticusisolates were compared by using the results obtained by the HPLC method. The comparison indicated that there was a highly statistically positive correlation between synthesised concentrations of AFB1 and AFG1 (r=0.82 μm), as well as between AFB2 and AFG2 (r=0.63 μm).The identity of all isolates previously characterised on the basis of morphological, ecological and toxigenic properties as species A. parasiticus was confirmed by the multiplex PCR detection method. Distinguishing between species A. parasiticus and morphologically similar species A. flavus was enabled by the application of the RFLP-PCR method, using restriction enzymes. This was also the first identification of this pathogenic species at the molecular level in Serbia., U toku četvorogodišnjih ispitivanja (2013-2016) identifikovana je, po prvi put u Srbiji, vrsta Asperigillus parasiticus kao prouzrokovač aspergilozne truleži zrna i klipa kukuruza u polju i skladištima. Sa ciljem da se ispita rasprostranjenost i diverzitet ove vrste obavljena su proučavanja morfoloških, patogenih, molekularnih, odgajivačkih i toksigenih svojstava 46 odabranih izolata ove vrste poreklom sa zrna kukuruza. Ispitivanjem makroskopskih morfoloških karakteristika izolata ukazano je na mali biodiverzitet proučavanih izolata. Kolonije izolata na PDA hranljivoj podlozi formirale su obilnu tamnozelenu, gustu prevlaku od micelije gljive, pamučastog izgleda. Svi izolati A. parasiticus obrazuju jednoćelijske, nazubljene konidije na MEA hranljivoj podlozi. Prečnik konidija ispitivanih izolata varirao je od 4,08μm do 6,86μm. Dvofaktorska analiza varijanse pokazala je statistički značajan uticaj interakcije ispitivanih izolata, svetlosnog režima i temperature (p<0.01) na dnevni porast prečnika kolonija. Test patogenosti ukazao je da su svi ispitivani izolati A. parasiticus patogeni na klipu kukuruza, kao i da postoji divergentnost u ispoljavanju virulentnosti. Poređenjem koncentracija sintetisanih mikotoksina kod izolata dobijenih Elisa testom, pokazali su da postoji statistički visoko značajna pozitivna korelacija između potencijala produkcije AFB1 i AFLA kod ispitivanih izolata (r=0,66 μm). Ispitivani izolati A.parasiticus koji su sintetisali aflatoksine (50%) su u najvećem procentu sintetisali AFB1 (84,78%), dok je procenat izolata koji su sintetisali AFG1 bila znatno manja (15,22%). Međutim, utvrđeno je osam izolata koji su sintetisali AFG1 u višim koncentracijama, u odnosu na AFB1. Poređenjem koncentracija mikotoksina kod izolata A. parasiticus, na osnovu rezultata HPLC metode, uočeno je da postoji visoko statistički značajna pozitivna korelacija između sintetisanih koncentracija AFB1 i AFG1 (r=0,82 μm), kao i između AFB2 i AFG2 (r=0,63 μm). Multiplex PCR detekcijom potvrđen je identitet svih izolata prethodno okarakterisanih na osnovu morfoloških, ekoloških, odgajivačkih i toksigenih karakteristika kao vrsta A. parasiticus. Primenom RFLP-PCR metode korišćenjem restrikcionih enzima, omogućeno je razlikovanje vrste A. parasiticus od morfološki slične vrste A. flavus, što je ujedno i prva identifikacija ove patogene vrste na molekularnom nivou u Srbiji.",
publisher = "Novi Sad, Univerzitet u Novom Sadu, Poljoprivredni fakultet",
title = "Aspergillus parasiticus Speare – toksigena vrsta gljive na zrnu kukuruza u proizvodnim uslovima Srbije, Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia"
}
Nikolić, M. (2019). Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia.

Novi Sad, Univerzitet u Novom Sadu, Poljoprivredni fakultet..
Nikolić M. Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia. 2019;
Nikolić Milica, "Aspergillus parasiticusSpeare –a toxigenic species on maize kernels underproduction conditions in Serbia" (2019)

Mycobiota and aflatoxin B1 in poultry feeds

Krnjaja, Vesna; Petrović, Tanja; Stanković, Slavica; Lukić, Miloš; Škrbić, Zdenka; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/742
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
T1  - Mikobiota i aflatoksin B1 u hrani za živinu
VL  - 35
IS  - 1
SP  - 61
EP  - 69
DO  - 10.2298/BAH1901061K
ER  - 
@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/742",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds, Mikobiota i aflatoksin B1 u hrani za živinu",
volume = "35",
number = "1",
pages = "61-69",
doi = "10.2298/BAH1901061K"
}
Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z. (2019). Mikobiota i aflatoksin B1 u hrani za živinu.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K
Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mikobiota i aflatoksin B1 u hrani za živinu. Biotechnology in Animal Husbandry. 2019;35(1):61-69
Krnjaja Vesna, Petrović Tanja, Stanković Slavica, Lukić Miloš, Škrbić Zdenka, Mandić Violeta, Bijelić Zorica, "Mikobiota i aflatoksin B1 u hrani za živinu" Biotechnology in Animal Husbandry, 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K .

Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains

Krnjaja, Vesna; Mandić, Violeta; Lukić, Miloš; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Vasić, Tanja
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/735
AB  - Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains
VL  - 116
SP  - 126
EP  - 131
DO  - 10.1016/j.cropro.2018.10.021
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lukić, Miloš and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Vasić, Tanja",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/735",
abstract = "Field trials were set up in the Belgrade area (Serbia) in 2013 and 2014 to determine the effect of plant density on the natural incidence of potentially toxigenic fungi and the level of mycotoxins, aflatoxin B-1 (AFB(1)), deoxynivalenol (DON) and fumonisins (FBs) in two Serbian maize hybrids of the FAO maturity group 700 (ZP 735 and NS Zenit). Three plant density treatments, namely, 55,000 plants ha(-1) (PD1), 64,000 plants ha(-1) (PD2) and 75,000 plants ha(-1) (PD3), were evaluated. The incidence of identified potentially toxigenic fungi of the genera Aspergillus, Fusarium, and Penicillium and the FB level increased significantly (P  lt = 0.01) at PD3. The effect of year was also significant (P  lt = 0.01) on the incidence of toxigenic fungi, as all fungal species had a higher incidence in 2014 than in 2013, with the exception of Aspergillus spp. that showed a significantly higher incidence in 2013. Levels of all tested mycotoxins were higher in 2014 than in 2013. The higher incidence of Fusarium graminearum, Fusarium proliferatum, Fusarium subglutinans, Fusarium verticillioides and Penicillium spp. and higher levels of AFB1 and FBs were observed in the hybrid ZP 735, whereas the hybrid NS Zenit had a higher incidence of Aspergillus spp. The effect of hybrids was not significant on the incidence of F. subglutinans and the DON level. These results indicated that the highest investigated plant density increased the fungal incidence and the FB level, especially in 2014. Although the two hybrids originated from the same FAO group, they differed in their effects on some toxigenic fungi and mycotoxins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains",
volume = "116",
pages = "126-131",
doi = "10.1016/j.cropro.2018.10.021"
}
Krnjaja, V., Mandić, V., Lukić, M., Bijelić, Z., Stanković, S., Obradović, A.,& Vasić, T. (2019). Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains.
Crop Protection
Elsevier Sci Ltd, Oxford., 116, 126-131.
https://doi.org/10.1016/j.cropro.2018.10.021
Krnjaja V, Mandić V, Lukić M, Bijelić Z, Stanković S, Obradović A, Vasić T. Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains. Crop Protection. 2019;116:126-131
Krnjaja Vesna, Mandić Violeta, Lukić Miloš, Bijelić Zorica, Stanković Slavica, Obradović Ana, Vasić Tanja, "Influence of plant density on toxigenic fungal and mycotoxin contamination of maize grains" Crop Protection, 116 (2019):126-131,
https://doi.org/10.1016/j.cropro.2018.10.021 .
7
3
4

Assessment stability of maize lines yield by GGE-biplot analysis

Božović, Dragan; Zivanović, Tomislav; Popović, Vera; Tatić, Mladen; Gospavić, Zagorka; Miloradović, Zoran; Dokić, Milorad; Stanković, Goran

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Božović, Dragan
AU  - Zivanović, Tomislav
AU  - Popović, Vera
AU  - Tatić, Mladen
AU  - Gospavić, Zagorka
AU  - Miloradović, Zoran
AU  - Dokić, Milorad
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/706
AB  - Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment stability of maize lines yield by GGE-biplot analysis
VL  - 50
IS  - 3
SP  - 755
EP  - 770
DO  - 10.2298/GENSR1803755B
ER  - 
@article{
author = "Božović, Dragan and Zivanović, Tomislav and Popović, Vera and Tatić, Mladen and Gospavić, Zagorka and Miloradović, Zoran and Dokić, Milorad and Stanković, Goran",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/706",
abstract = "Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment stability of maize lines yield by GGE-biplot analysis",
volume = "50",
number = "3",
pages = "755-770",
doi = "10.2298/GENSR1803755B"
}
Božović, D., Zivanović, T., Popović, V., Tatić, M., Gospavić, Z., Miloradović, Z., Dokić, M.,& Stanković, G. (2018). Assessment stability of maize lines yield by GGE-biplot analysis.
Genetika
Društvo genetičara Srbije, Beograd., 50(3), 755-770.
https://doi.org/10.2298/GENSR1803755B
Božović D, Zivanović T, Popović V, Tatić M, Gospavić Z, Miloradović Z, Dokić M, Stanković G. Assessment stability of maize lines yield by GGE-biplot analysis. Genetika. 2018;50(3):755-770
Božović Dragan, Zivanović Tomislav, Popović Vera, Tatić Mladen, Gospavić Zagorka, Miloradović Zoran, Dokić Milorad, Stanković Goran, "Assessment stability of maize lines yield by GGE-biplot analysis" Genetika, 50, no. 3 (2018):755-770,
https://doi.org/10.2298/GENSR1803755B .
5
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Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Bozić, Manja

(Mdpi, Basel, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Bozić, Manja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/702
AB  - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
PB  - Mdpi, Basel
T2  - Toxins
T1  - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
VL  - 10
IS  - 11
DO  - 10.3390/toxins10110460
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Bozić, Manja",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/702",
abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.",
publisher = "Mdpi, Basel",
journal = "Toxins",
title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia",
volume = "10",
number = "11",
doi = "10.3390/toxins10110460"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T.,& Bozić, M. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia.
Toxins
Mdpi, Basel., 10(11).
https://doi.org/10.3390/toxins10110460
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Bozić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. Toxins. 2018;10(11)
Krnjaja Vesna, Mandić Violeta, Bijelić Zorica, Stanković Slavica, Obradović Ana, Petrović Tanja, Bozić Manja, "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" Toxins, 10, no. 11 (2018),
https://doi.org/10.3390/toxins10110460 .
3
1
2

Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji

Obradović, Ana

(Универзитет у Београду, Пољопривредни факултет, 2018)

TY  - BOOK
AU  - Obradović, Ana
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6037
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18357/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50361615
UR  - http://nardus.mpn.gov.rs/123456789/10079
UR  - http://rik.mrizp.rs/handle/123456789/756
AB  - Za proučavanje diverziteta vrste F. graminearum odabran je 101 izolat izkolekcije Laboratorije za fitopatologiju Instituta za kukuruz "Zemun Polje" koji suprethodno identifikovani konvencionalnim metodama na osnovu makroskopskih imikroskopskih svojstava. Izolati su prikupljeni u periodu od 1993. do 2015. godine, aporeklom iz uzoraka pšenice (42), kukuruza (37) i ječma (22) iz 41 lokaliteta nateritoriji Srbije. Sa ciljem da se dobije uvid o rasprostranjenosti, prisustvu i diverzitetuvrsta Fusarium graminearum kompleksa (Fg) kao i o trihotecenskim hemotipovima uSrbiji, obavljena su proučavanja morfoloških, patogenih, toksigenih i filogenetskihsvojstava odabranih izolata. Na osnovu svih proučenih svojstava utvrđeno je prisustvotri vrste u okviru Fg kompleksa: Fusarium graminearum sensu stricto, Fusariumboothii i Fusarium vorosii. Po prvi put u Srbiji, detektovano je prisustvo F. boothii i F.vorosii patogena strnih žita i kukuruza u našoj zemlji.Na osnovu morfoloških, patogenih i toksigenih svojstava uočen je velikidiverzitet ispitivanih izolata. Rezultati analize morfoloških svojstava izolata F.graminearum ss, F. boothii i F. vorosii, ukazuju da ne postoje razlike među njima.Izolati vrste F. boothii i F. vorosii ispoljili su generalno slabiju patogenost u poređenjusa ostalim izolatima. Analiza potencijala produkcije mikotoksina deoksinivalenola izearalenona ELISA i HPLC metodom ukazala je na varijabilnost između ispitivanihizolata. Određivanjem trihotecenskih hemotipova hemijskom analizom utvrđeno je da uSrbiji postoje dva hemotipa - 15ADON (dominantan) i 3ADON.Za razliku od hemijskih, molekularnim analizama na osnovu TRI3 i TRI12 gena,utvrđeno je prisustvo samo 15ADON hemotipa. Dobijeni rezultati o biodiverzitetuhemotipova su prvi nalaz u agroekološkim uslovima Srbije. Analiza nukleotidnihsekvenci tri genomska regiona (TEF−1α, β−tubulin i histon H3), pokazala je da postojerazličiti potencijali za razdvajanje vrsta u okviru Fg kompleksa. Rezultati ove disertacijeukazali su na postojanje dve vrste u okviru Fg kompleksa (F. boothii i F. vorosii), kojedo sada nisu identifikovane na teritoriji Srbije. Sekvenciranje ovih genomskih regionaodabranih izolata i njihovo poređenje sa referentnim izolatima kao i poređenje saizolatima Fg kompleksa u svetu doprinelo je boljem poznavanju biodiverziteta ovihvrsta u Srbiji.
AB  - One hundred and one isolates of the F. graminearum species have been isolatedfrom the collection of the Laboratory of Phytopathology of the Maize ResearchInstitute, Zemun Polje to study the species diversity. These isolates had been previouslyidentified using conventional methods based on macroscopic and microscopicproperties. Isolates, originating from samples of wheat (42), maize (37) and barley (22),were collected from 41 locations in Serbia in the period from 1993 to 2015. In order togain insight into the distribution, presence and diversity of the Fusarium graminearumspecies complex (Fg), as well as into thrichotecene chemotypes in Serbia, studies of themorphological, pathogenic, toxigenic and phylogenetic properties of the selectedisolates were carried out. According to all observed properties, the presence of the threefollowing species was determined within the Fg complex: Fusarium graminearumsensu stricto, Fusarium boothii and Fusarium vorosii. F. boothii and F. vorosii,pathogens of small grains and maize in our country, were detected for the first time inSerbia.Based on morphological, pathogenic and toxigenic properties, a great diversityof analysed isolates was observed. Results of analyses of morphological properties ofthe isolates of F. graminearum ss, F. boothii and F. vorosii, pointed out that there hadbeen no differences among them. Isolates of F. boothii and F. vorosii species exhibitedgenerally less pathogenicity than remaining isolates. The analysis of the potential for theproduction of mycotoxins deoxynivalenol and zearalenone by the ELISA and the HPLCmethod pointed out to the variability among analysed isolates. The determination oftrichothecene chemotypes done by chemical analyses confirmed the presence of twochemotypes in Serbia- 15ADON (dominant) and 3ADON. In contrast to chemicalanalyses, the molecular analyses, based on the TRI3 and TRI12 genes, established thepresence of only 15ADON chemotype. The obtained results on the biodiversity ofchemotypes are the first finding under agroecological conditions of Serbia. The analysisof nucleotide sequences of the three genomic regions (TEF−1α, β−tubulin and histoneH3), showed the existence of different potentials for separation of species within the Fgcomplex. The results presented in this PhD thesis point out to the existence of twospecies (F. boothii and F. vorosii) within the Fg complex, which have not yet beenidentified in the territory of Serbia. Sequencing these portions of the genome of theselected isolates and their comparison with the reference isolates, as well as, thecomparison with the Fg complex isolates in the world contributed to a better knowledgeof the biodiversity of these species in Serbia.
PB  - Универзитет у Београду, Пољопривредни факултет
T2  - Универзитет у Београду
T1  - Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji
ER  - 
@phdthesis{
author = "Obradović, Ana",
year = "2018",
url = "http://eteze.bg.ac.rs/application/showtheses?thesesId=6037, https://fedorabg.bg.ac.rs/fedora/get/o:18357/bdef:Content/download, http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50361615, http://nardus.mpn.gov.rs/123456789/10079, http://rik.mrizp.rs/handle/123456789/756",
abstract = "Za proučavanje diverziteta vrste F. graminearum odabran je 101 izolat izkolekcije Laboratorije za fitopatologiju Instituta za kukuruz "Zemun Polje" koji suprethodno identifikovani konvencionalnim metodama na osnovu makroskopskih imikroskopskih svojstava. Izolati su prikupljeni u periodu od 1993. do 2015. godine, aporeklom iz uzoraka pšenice (42), kukuruza (37) i ječma (22) iz 41 lokaliteta nateritoriji Srbije. Sa ciljem da se dobije uvid o rasprostranjenosti, prisustvu i diverzitetuvrsta Fusarium graminearum kompleksa (Fg) kao i o trihotecenskim hemotipovima uSrbiji, obavljena su proučavanja morfoloških, patogenih, toksigenih i filogenetskihsvojstava odabranih izolata. Na osnovu svih proučenih svojstava utvrđeno je prisustvotri vrste u okviru Fg kompleksa: Fusarium graminearum sensu stricto, Fusariumboothii i Fusarium vorosii. Po prvi put u Srbiji, detektovano je prisustvo F. boothii i F.vorosii patogena strnih žita i kukuruza u našoj zemlji.Na osnovu morfoloških, patogenih i toksigenih svojstava uočen je velikidiverzitet ispitivanih izolata. Rezultati analize morfoloških svojstava izolata F.graminearum ss, F. boothii i F. vorosii, ukazuju da ne postoje razlike među njima.Izolati vrste F. boothii i F. vorosii ispoljili su generalno slabiju patogenost u poređenjusa ostalim izolatima. Analiza potencijala produkcije mikotoksina deoksinivalenola izearalenona ELISA i HPLC metodom ukazala je na varijabilnost između ispitivanihizolata. Određivanjem trihotecenskih hemotipova hemijskom analizom utvrđeno je da uSrbiji postoje dva hemotipa - 15ADON (dominantan) i 3ADON.Za razliku od hemijskih, molekularnim analizama na osnovu TRI3 i TRI12 gena,utvrđeno je prisustvo samo 15ADON hemotipa. Dobijeni rezultati o biodiverzitetuhemotipova su prvi nalaz u agroekološkim uslovima Srbije. Analiza nukleotidnihsekvenci tri genomska regiona (TEF−1α, β−tubulin i histon H3), pokazala je da postojerazličiti potencijali za razdvajanje vrsta u okviru Fg kompleksa. Rezultati ove disertacijeukazali su na postojanje dve vrste u okviru Fg kompleksa (F. boothii i F. vorosii), kojedo sada nisu identifikovane na teritoriji Srbije. Sekvenciranje ovih genomskih regionaodabranih izolata i njihovo poređenje sa referentnim izolatima kao i poređenje saizolatima Fg kompleksa u svetu doprinelo je boljem poznavanju biodiverziteta ovihvrsta u Srbiji., One hundred and one isolates of the F. graminearum species have been isolatedfrom the collection of the Laboratory of Phytopathology of the Maize ResearchInstitute, Zemun Polje to study the species diversity. These isolates had been previouslyidentified using conventional methods based on macroscopic and microscopicproperties. Isolates, originating from samples of wheat (42), maize (37) and barley (22),were collected from 41 locations in Serbia in the period from 1993 to 2015. In order togain insight into the distribution, presence and diversity of the Fusarium graminearumspecies complex (Fg), as well as into thrichotecene chemotypes in Serbia, studies of themorphological, pathogenic, toxigenic and phylogenetic properties of the selectedisolates were carried out. According to all observed properties, the presence of the threefollowing species was determined within the Fg complex: Fusarium graminearumsensu stricto, Fusarium boothii and Fusarium vorosii. F. boothii and F. vorosii,pathogens of small grains and maize in our country, were detected for the first time inSerbia.Based on morphological, pathogenic and toxigenic properties, a great diversityof analysed isolates was observed. Results of analyses of morphological properties ofthe isolates of F. graminearum ss, F. boothii and F. vorosii, pointed out that there hadbeen no differences among them. Isolates of F. boothii and F. vorosii species exhibitedgenerally less pathogenicity than remaining isolates. The analysis of the potential for theproduction of mycotoxins deoxynivalenol and zearalenone by the ELISA and the HPLCmethod pointed out to the variability among analysed isolates. The determination oftrichothecene chemotypes done by chemical analyses confirmed the presence of twochemotypes in Serbia- 15ADON (dominant) and 3ADON. In contrast to chemicalanalyses, the molecular analyses, based on the TRI3 and TRI12 genes, established thepresence of only 15ADON chemotype. The obtained results on the biodiversity ofchemotypes are the first finding under agroecological conditions of Serbia. The analysisof nucleotide sequences of the three genomic regions (TEF−1α, β−tubulin and histoneH3), showed the existence of different potentials for separation of species within the Fgcomplex. The results presented in this PhD thesis point out to the existence of twospecies (F. boothii and F. vorosii) within the Fg complex, which have not yet beenidentified in the territory of Serbia. Sequencing these portions of the genome of theselected isolates and their comparison with the reference isolates, as well as, thecomparison with the Fg complex isolates in the world contributed to a better knowledgeof the biodiversity of these species in Serbia.",
publisher = "Универзитет у Београду, Пољопривредни факултет",
journal = "Универзитет у Београду",
title = "Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji"
}
Obradović, A. (2018). Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji.
Универзитет у Београду
Универзитет у Београду, Пољопривредни факултет..
Obradović A. Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji. Универзитет у Београду. 2018;
Obradović Ana, "Diverzitet kompleksa vrste Fusarium graminearum patogena strnih žita i kukuruza u Srbiji" Универзитет у Београду (2018)

Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat

Nikolić, Milica; Nikolić, Ana; Savić, Iva; Petrović, Tanja; Stanković, Slavica; Jauković, Marko; Bagi, Ferenc

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Savić, Iva
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Jauković, Marko
AU  - Bagi, Ferenc
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/709
AB  - The species of the genus Aspergillus, A. flavus and A. parasiticus, are the most aflatoxin-producing fungi. All previous studies carried out under the production conditions of Serbia showed no presence of A. parasiticus on wheat kernel. On the basis of changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, which favour increased frequency of Aspergillus spp., we assumed that this pathogen can also be present in Serbia. The significance of direct losses as a consequence of wheat kernel infection, as well as potential contamination with aflatoxins, have pointed out to the need to determine the presence of toxigenic potential of A. flavus and A. parasiticus isolates originating from Serbia. For that purpose, wheat kernel samples were collected in nine locations. According to morphological, toxicological and molecular traits of isolated fungi, the presence of A. flavus and A. parasiticus was confirmed. This is the first time that A. parasiticus was identified on wheat under climatic conditions in Serbia. This study indicates that these pathogens may be a potential danger in wheat production in the region of Serbia. This danger will be much more certain if global climatic changes continue as they will provide more intensive development of these pathogens.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat
VL  - 50
IS  - 1
SP  - 143
EP  - 152
DO  - 10.2298/GENSR1801143N
ER  - 
@article{
author = "Nikolić, Milica and Nikolić, Ana and Savić, Iva and Petrović, Tanja and Stanković, Slavica and Jauković, Marko and Bagi, Ferenc",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/709",
abstract = "The species of the genus Aspergillus, A. flavus and A. parasiticus, are the most aflatoxin-producing fungi. All previous studies carried out under the production conditions of Serbia showed no presence of A. parasiticus on wheat kernel. On the basis of changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, which favour increased frequency of Aspergillus spp., we assumed that this pathogen can also be present in Serbia. The significance of direct losses as a consequence of wheat kernel infection, as well as potential contamination with aflatoxins, have pointed out to the need to determine the presence of toxigenic potential of A. flavus and A. parasiticus isolates originating from Serbia. For that purpose, wheat kernel samples were collected in nine locations. According to morphological, toxicological and molecular traits of isolated fungi, the presence of A. flavus and A. parasiticus was confirmed. This is the first time that A. parasiticus was identified on wheat under climatic conditions in Serbia. This study indicates that these pathogens may be a potential danger in wheat production in the region of Serbia. This danger will be much more certain if global climatic changes continue as they will provide more intensive development of these pathogens.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat",
volume = "50",
number = "1",
pages = "143-152",
doi = "10.2298/GENSR1801143N"
}
Nikolić, M., Nikolić, A., Savić, I., Petrović, T., Stanković, S., Jauković, M.,& Bagi, F. (2018). Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat.
Genetika
Društvo genetičara Srbije, Beograd., 50(1), 143-152.
https://doi.org/10.2298/GENSR1801143N
Nikolić M, Nikolić A, Savić I, Petrović T, Stanković S, Jauković M, Bagi F. Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat. Genetika. 2018;50(1):143-152
Nikolić Milica, Nikolić Ana, Savić Iva, Petrović Tanja, Stanković Slavica, Jauković Marko, Bagi Ferenc, "Differentiation between aspergillus flavus and aspergillus parasiticus isolates originated from wheat" Genetika, 50, no. 1 (2018):143-152,
https://doi.org/10.2298/GENSR1801143N .
2
3
2

Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence

Obradović, Ana; Krnjaja, Vesna; Nikolić, Milica; Delibašić, Goran; Filipović, Milomir; Stanković, Goran; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Delibašić, Goran
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/738
AB  - Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.
AB  - Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence
T1  - Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava
VL  - 34
IS  - 4
SP  - 469
EP  - 480
DO  - 10.2298/BAH1804469O
ER  - 
@article{
author = "Obradović, Ana and Krnjaja, Vesna and Nikolić, Milica and Delibašić, Goran and Filipović, Milomir and Stanković, Goran and Stanković, Slavica",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/738",
abstract = "Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species., Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence, Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava",
volume = "34",
number = "4",
pages = "469-480",
doi = "10.2298/BAH1804469O"
}
Obradović, A., Krnjaja, V., Nikolić, M., Delibašić, G., Filipović, M., Stanković, G.,& Stanković, S. (2018). Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(4), 469-480.
https://doi.org/10.2298/BAH1804469O
Obradović A, Krnjaja V, Nikolić M, Delibašić G, Filipović M, Stanković G, Stanković S. Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava. Biotechnology in Animal Husbandry. 2018;34(4):469-480
Obradović Ana, Krnjaja Vesna, Nikolić Milica, Delibašić Goran, Filipović Milomir, Stanković Goran, Stanković Slavica, "Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava" Biotechnology in Animal Husbandry, 34, no. 4 (2018):469-480,
https://doi.org/10.2298/BAH1804469O .
4

Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia

Krnjaja, Vesna; Stanković, Slavica; Lukić, Miloš; Mićić, Nenad; Petrović, Tanja; Bijelić, Zorica; Mandić, Violeta

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/720
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
VL  - 34
IS  - 2
SP  - 239
EP  - 249
DO  - 10.2298/bah1802239K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/720",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
volume = "34",
number = "2",
pages = "239-249",
doi = "10.2298/bah1802239K"
}
Krnjaja, V., Stanković, S., Lukić, M., Mićić, N., Petrović, T., Bijelić, Z.,& Mandić, V. (2018). Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K
Krnjaja V, Stanković S, Lukić M, Mićić N, Petrović T, Bijelić Z, Mandić V. Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji. Biotechnology in Animal Husbandry. 2018;34(2):239-249
Krnjaja Vesna, Stanković Slavica, Lukić Miloš, Mićić Nenad, Petrović Tanja, Bijelić Zorica, Mandić Violeta, "Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji" Biotechnology in Animal Husbandry, 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K .
1

In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia

Bočarov-Stančić, Aleksandra; Lopičić, Zorica R.; Bodroža-Solarov, Marija I.; Stanković, Slavica; Janković, Snežana; Milojković, Jelena V.; Krulj, Jelena A.

(Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad, 2018)

TY  - JOUR
AU  - Bočarov-Stančić, Aleksandra
AU  - Lopičić, Zorica R.
AU  - Bodroža-Solarov, Marija I.
AU  - Stanković, Slavica
AU  - Janković, Snežana
AU  - Milojković, Jelena V.
AU  - Krulj, Jelena A.
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/718
AB  - Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties.
AB  - Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia
T1  - In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije
VL  - 45
IS  - 7
SP  - 87
EP  - 96
DO  - 10.5937/FFR1802087B
ER  - 
@article{
author = "Bočarov-Stančić, Aleksandra and Lopičić, Zorica R. and Bodroža-Solarov, Marija I. and Stanković, Slavica and Janković, Snežana and Milojković, Jelena V. and Krulj, Jelena A.",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/718",
abstract = "Aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON) and T-2 toxin are the most extensively studied toxic fungal metabolites. Once mycotoxins enter the food/feed production chain keeping their toxic characteristics, it is very difficult to remove or eliminate them. One of promising methods to reduce mycotoxins in contaminated food/feedstuffs is the use of mycotoxin binders. This paper presents the results of in vitro investigations of mineral mycotoxin binders (bentonite - BEN, diatomite - DIA and zeolite - ZEO), and organic mycotoxin binders - agricultural waste materials (Myriophillium spicatum, peach and sour cherry pits). Chemical compositions of the adsorbents have showed that they do not consist of elements toxic to the animals. Inorganic adsorbents (BEN, DIA and ZEO) tested in vitro were better binders of AFB1 (94.97% - 96.90%), while the biosorbents were more efficient in adsorption of OTA (19.98% - 66.66%), ZON (33.33% - 75.00%) and T-2 toxin (16.67% - 50.00%). Inorganic adsorbents and organic waste materials expressed similar binding capacity for DON in vitro, with the exception of M. spicatum that did not at all adsorb this type B trichothecene. Our results indicate that feed contamination with different types of mycotoxins might be diminished by a product that combines different inorganic and organic adsorbents with diverse mycotoxin binding properties., Aflatoksin B1 (AFB1), ohratoksin A (OTA), zearalenon (ZON), dezoksinivalenol (DON) i T-2 toksin su najviše izučavani toksični metaboliti gljiva. Kada mikotoksini uđu u proizvodni lanac za hranu/hranu za životinje, zadržavajući svoje toksične karakteristike, teško ih je ukloniti ili eliminisati. Jedna od obećavajućih metoda za smanjenje nivoa mikotoksina u kontaminiranoj hrani/hrani za životinje je korišćenje mikotoksinskih veziva. Ovaj rad predstavlja rezultate in vitro istraživanja mineralnih mikotoksinskih veziva (bentonit - BEN, diatomit - DIA i zeolit - ZEO) i organskih veziva mikotoksina - poljoprivrednog otpadnog materijala (Myriophillium spicatum, koštice breskve i višnje). Hemijski sastavi adsorbenata pokazali su da ne sadrže elemente toksične za životinje. Neorganski adsorbenti (BEN, DIA i ZEO) testirani in vitro bolje su vezivali AFB1 (94,97% - 96,90%), dok su biosorbenti bili efikasniji u adsorpciji OTA (19,98% - 66,66%), ZON-a (33,33% - 75,00% ) i T-2 toksina (16,67% - 50,00%). Neorganski adsorbenti i organski otpadni materijali su pokazali sličan kapacitet in vitro vezivanja DON-a, sa izuzetkom M. spicatum koji uopšte nije adsorbovao ovaj trihotecen tipa B. Naši rezultati koji su prikazani ovde pokazuju da zagađivanje hrane i hrane za životinje različitim vrstama mikotoksina može biti smanjeno dodavanjem preparata dobijenog kombinacijom različitih neorganskih i organskih adsorbenata koji poseduju različite karakteristike vezivanja mikotoksina.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "In vitro removing of mycotoxins by using different inorganic adsorbents and organic waste materials from Serbia, In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije",
volume = "45",
number = "7",
pages = "87-96",
doi = "10.5937/FFR1802087B"
}
Bočarov-Stančić, A., Lopičić, Z. R., Bodroža-Solarov, M. I., Stanković, S., Janković, S., Milojković, J. V.,& Krulj, J. A. (2018). In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije.
Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 45(7), 87-96.
https://doi.org/10.5937/FFR1802087B
Bočarov-Stančić A, Lopičić ZR, Bodroža-Solarov MI, Stanković S, Janković S, Milojković JV, Krulj JA. In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije. Food and Feed Research. 2018;45(7):87-96
Bočarov-Stančić Aleksandra, Lopičić Zorica R., Bodroža-Solarov Marija I., Stanković Slavica, Janković Snežana, Milojković Jelena V., Krulj Jelena A., "In vitro uklanjanje mikotoksina korišćenjem različitih neorganskih adsorbenata i organskih otpadnih materijala iz Srbije" Food and Feed Research, 45, no. 7 (2018):87-96,
https://doi.org/10.5937/FFR1802087B .

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica; Nikolić, Ana; Ignjatović-Micić, Dragana; Krnjaja, Vesna; Stepanović, Jelena; Duduk, Bojan

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Krnjaja, Vesna
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/682
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Nikolić, Ana and Ignjatović-Micić, Dragana and Krnjaja, Vesna and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/682",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O"
}
Obradović, A., Stanković, S., Nikolić, A., Ignjatović-Micić, D., Krnjaja, V., Stepanović, J.,& Duduk, B. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia.
Genetika
Društvo genetičara Srbije, Beograd., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
Obradović A, Stanković S, Nikolić A, Ignjatović-Micić D, Krnjaja V, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. Genetika. 2017;49(1):355-364
Obradović Ana, Stanković Slavica, Nikolić Ana, Ignjatović-Micić Dragana, Krnjaja Vesna, Stepanović Jelena, Duduk Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" Genetika, 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O .
3
3
3

Fungal contamination of maize grain samples with a special focus on toxigenic genera

Krnjaja, Vesna; Stanojković, Aleksandar; Stanković, Slavica; Lukić, Miloš; Bijelić, Zorica; Mandić, Violeta; Mićić, Nenad

(Institut za stočarstvo, Beograd, 2017)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanojković, Aleksandar
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Mićić, Nenad
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/664
AB  - In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi.
AB  - Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal contamination of maize grain samples with a special focus on toxigenic genera
T1  - Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove
VL  - 33
IS  - 2
SP  - 233
EP  - 241
DO  - 10.2298/BAH1702233K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanojković, Aleksandar and Stanković, Slavica and Lukić, Miloš and Bijelić, Zorica and Mandić, Violeta and Mićić, Nenad",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/664",
abstract = "In this study, the total fungal count and contamination with toxigenic fungi from Aspergillus, Fusarium and Penicillium genera of 127 maize grain samples collected from animal farms in subrbs of Belgrade area during 2012-2015, were determined. The total fungal count was determined using a dilution method, and standard mycological procedures were used to identify potential toxigenic fungi genera. In the tested samples of maize grains, the total fungal count was from 1 x 101 to 3 x 106 cfu g-1. No statistically significant differences between investigated years in regard to the mean total fungal count were determined. According to the Regulation on the quality of animal feed of the Republic of Serbia, the total fungal count above permitted limit (2 x 105 cfu g-1) was established in 9.52, 7.89, 20.69 and 55.56% tested samples in 2012, 2013, 2014 and 2015, respectively. Potentially toxigenic fungi from Aspergillus, Fusarium and Penicillim genera have been identified as the most common in all the samples. In most of the samples, based on the average value for the four-year period (2012-2015), Fusarium species (92.22%) have been identified, followed by the species of the genera Aspergillus (80.83%) and Penicillium (48.68%). A weak positive correlation was established between the moisture content of the samples and the total fungal count in 2012 (r=0.41), in 2013 (r=0.27) and in 2014 (r=0.36) and the medium positive correlation (r=0.61) in 2015. Based on the results of mycological analysis of grain maize it can be concluded that the test samples in a relatively large number did not meet the criteria of hygienic quality. Therefore, regular and continuous control of the mycological quality of maize grain as the most important nutrient in animal nutrition is necessary as a preventive measure to reduce and control contamination of grain with mycotoxigenic fungi., Ukupan broj gljiva i kontaminacija s potencijalno toksigenim vrstama iz rodova Aspergillus, Fusarium i Penicillium određivani su u 127 uzoraka zrna kukuruza koji su sakupljeni na farmama u okolini Beograda tokom četvorogodišnjeg perioda (2012-2015). Primenom metode razređenja određen je ukupan broj gljiva, dok su standardne mikološke metode korišćene za identifikaciju potencijalno toksigenih rodova gljiva. U ispitivanim uzorcima zrna kukuruza ukupan broj gljiva je bio od 1 x 101 do 3 x 106 cfu g-1. Između ispitivanih godina nisu ustanovljene statističke značajne razlike u prosečnim vrednostima ukupnog broja gljiva. Prema Pravilniku Republike Srbije o kvalitetu hrane za životinje, u hranivima biljnog porekla, ukupan broj gljiva iznad dozvoljenog limita (2 x 105 cfu g-1) ustanovljen je u 9,52, 7,89, 20,69 i 55,56% ispitivanih uzoraka u 2012., 2013., 2014. i 2015. godini, respektivno. Od potencijalno toksigenih gljiva identifikovane su Aspergillus, Fusarium i Penicillim vrste kao najučestalije u svim ispitivanim uzorcima. U najvećem broju uzoraka, na osnovu prosečnih vrednosti u četvorogodišnjem periodu (2012-2015), identifikovane su Fusarium vrste (92.22%), zatim vrste iz rodova Aspergillus (80.83%) i Penicillium (48.68%). Između sadržaja vlage ispitivanih uzoraka i ukupnog broja gljiva ustanovljena je slaba pozitivna korelacija u 2012. (r=0.41), 2013. (r=0.27) i 2014. godini (r=0.36) i srednje pozitivna korelacija (r=0.61) u 2015. godini. Na osnovu dobijenih rezultata mikološke analize zrna kukuruza može se zaključiti da ispitivani uzorci u relativno velikom broju ne zadovoljavaju kriterijume higijenskog kvaliteta. Zbog toga, redovna i stalna kontrola mikološkog kvaliteta zrna kukuruza kao najvažnijeg hraniva u ishrani životinja je neophodna preventivna mera za smanjenje i kontrolu kontaminacije zrna s mikotoksigenim gljivama.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal contamination of maize grain samples with a special focus on toxigenic genera, Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove",
volume = "33",
number = "2",
pages = "233-241",
doi = "10.2298/BAH1702233K"
}
Krnjaja, V., Stanojković, A., Stanković, S., Lukić, M., Bijelić, Z., Mandić, V.,& Mićić, N. (2017). Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 33(2), 233-241.
https://doi.org/10.2298/BAH1702233K
Krnjaja V, Stanojković A, Stanković S, Lukić M, Bijelić Z, Mandić V, Mićić N. Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove. Biotechnology in Animal Husbandry. 2017;33(2):233-241
Krnjaja Vesna, Stanojković Aleksandar, Stanković Slavica, Lukić Miloš, Bijelić Zorica, Mandić Violeta, Mićić Nenad, "Kontaminacija uzoraka zrna kukuruza gljivama s posebnim osvrtom na toksigene rodove" Biotechnology in Animal Husbandry, 33, no. 2 (2017):233-241,
https://doi.org/10.2298/BAH1702233K .
4

Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels

Krstović, Saša Z.; Jakšić, Sandra M.; Bočarov-Stančić, Aleksandra; Stanković, Slavica; Janković, Snežana; Jajić, Igor M.

(Matica srpska, Novi Sad, 2017)

TY  - JOUR
AU  - Krstović, Saša Z.
AU  - Jakšić, Sandra M.
AU  - Bočarov-Stančić, Aleksandra
AU  - Stanković, Slavica
AU  - Janković, Snežana
AU  - Jajić, Igor M.
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/662
AB  - The production of fumonisins by potentially toxigenic Fusarium verticillioides isolates originating from Serbian maize and wheat kernels was tested in vitro. A total of six F. verticillioides isolates were incubated on yeast extract sucrose medium (YESA) for 4 weeks at 25 °C in the dark. Their toxin production potential was tested by applying a modified HPLC method for determination of fumonisins in cereals, since the TLC method gave no results. Analyses were performed on a HPLC-FLD system after sample extraction from YESA and extract clean-up on a SPE column. Although the isolates were tested for fumonisin B1, B2 and B3, only fumonisin B1 was detected. The results showed that all tested isolates had toxigenic potential for fumonisin B1 production. The average fumonisin B1 production of the isolates ranged from 7 to 289 µg/kg, thus indicating a highly variable toxigenic potential among the isolates. Isolate 1282 expressed the highest toxigenic potential for fumonisin B1 production (289 µg/kg), while isolate 2533/A showed a questionable potential for fumonisin production (7 µg/kg).
AB  - Proizvodnja fumonizina kod potencijalno toksikogenih Fusarium verticillioides izolata sa zrna kukuruza i pšenice poreklom iz Srbije testiran je in vitro. Ukupno šest izolata F. verticillioides inkubirano je na agarizovanoj podlozi sa ekstraktom kvasca i saharozom (YESA) tokom četiri nedelje, u mraku na 25 °C. Kapaciteti odabranih izolata za proizvodnju toksina detektovani su primenom modifikovane HPLC metode za određivanje fumonizina u zrnu žitarica, jer brza trijažna TLC metoda nije dala pozitivne rezultate. Analiza je izvedena na HPLC-FLD sistemu nakon ekstrakcije uzorka iz YESA kultura izolata i prečišćavanja ekstrakta na SPE koloni. Sve analize su urađene u tri ponavljanja. Iako su izolati F. verticillioides testirani na prisustvo fumonizina B1, B2 i B3, samo je fumonizin B1 bio detektovan. Rezultati su pokazali da svi testirani izolati imaju potencijal u sintezi fumonizina B1. Prosečna vrednost proizvedenog fumonizina B1 kod izolata F. verticillioides kretala se od 7 do 289 µg/kg, što ukazuje na izuzetno varijabilan toksigeni potencijal istih izolata. Izolat označen sa 1282 pokazao je najveći potencijal za biosintezu fumonizina B1 (289 µg/kg), dok je izolat 2533/A ispoljio diskutabilan potencijal za proizvodnju istog fumonizina (7 µg/kg).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels
T1  - Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije
IS  - 133
SP  - 71
EP  - 78
DO  - 10.2298/ZMSPN1733071K
ER  - 
@article{
author = "Krstović, Saša Z. and Jakšić, Sandra M. and Bočarov-Stančić, Aleksandra and Stanković, Slavica and Janković, Snežana and Jajić, Igor M.",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/662",
abstract = "The production of fumonisins by potentially toxigenic Fusarium verticillioides isolates originating from Serbian maize and wheat kernels was tested in vitro. A total of six F. verticillioides isolates were incubated on yeast extract sucrose medium (YESA) for 4 weeks at 25 °C in the dark. Their toxin production potential was tested by applying a modified HPLC method for determination of fumonisins in cereals, since the TLC method gave no results. Analyses were performed on a HPLC-FLD system after sample extraction from YESA and extract clean-up on a SPE column. Although the isolates were tested for fumonisin B1, B2 and B3, only fumonisin B1 was detected. The results showed that all tested isolates had toxigenic potential for fumonisin B1 production. The average fumonisin B1 production of the isolates ranged from 7 to 289 µg/kg, thus indicating a highly variable toxigenic potential among the isolates. Isolate 1282 expressed the highest toxigenic potential for fumonisin B1 production (289 µg/kg), while isolate 2533/A showed a questionable potential for fumonisin production (7 µg/kg)., Proizvodnja fumonizina kod potencijalno toksikogenih Fusarium verticillioides izolata sa zrna kukuruza i pšenice poreklom iz Srbije testiran je in vitro. Ukupno šest izolata F. verticillioides inkubirano je na agarizovanoj podlozi sa ekstraktom kvasca i saharozom (YESA) tokom četiri nedelje, u mraku na 25 °C. Kapaciteti odabranih izolata za proizvodnju toksina detektovani su primenom modifikovane HPLC metode za određivanje fumonizina u zrnu žitarica, jer brza trijažna TLC metoda nije dala pozitivne rezultate. Analiza je izvedena na HPLC-FLD sistemu nakon ekstrakcije uzorka iz YESA kultura izolata i prečišćavanja ekstrakta na SPE koloni. Sve analize su urađene u tri ponavljanja. Iako su izolati F. verticillioides testirani na prisustvo fumonizina B1, B2 i B3, samo je fumonizin B1 bio detektovan. Rezultati su pokazali da svi testirani izolati imaju potencijal u sintezi fumonizina B1. Prosečna vrednost proizvedenog fumonizina B1 kod izolata F. verticillioides kretala se od 7 do 289 µg/kg, što ukazuje na izuzetno varijabilan toksigeni potencijal istih izolata. Izolat označen sa 1282 pokazao je najveći potencijal za biosintezu fumonizina B1 (289 µg/kg), dok je izolat 2533/A ispoljio diskutabilan potencijal za proizvodnju istog fumonizina (7 µg/kg).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels, Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije",
number = "133",
pages = "71-78",
doi = "10.2298/ZMSPN1733071K"
}
Krstović, S. Z., Jakšić, S. M., Bočarov-Stančić, A., Stanković, S., Janković, S.,& Jajić, I. M. (2017). Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije.
Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(133), 71-78.
https://doi.org/10.2298/ZMSPN1733071K
Krstović SZ, Jakšić SM, Bočarov-Stančić A, Stanković S, Janković S, Jajić IM. Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije. Zbornik Matice srpske za prirodne nauke. 2017;(133):71-78
Krstović Saša Z., Jakšić Sandra M., Bočarov-Stančić Aleksandra, Stanković Slavica, Janković Snežana, Jajić Igor M., "Potencijal za proizvodnju fumonizina kod izolata Fusarium verticillioides sa zrna kukuruza i pšenice iz Srbije" Zbornik Matice srpske za prirodne nauke, no. 133 (2017):71-78,
https://doi.org/10.2298/ZMSPN1733071K .

Pathogenicity of Aspergillus spp. isolates originating from Serbia

Nikolić, Milica; Savić, Iva; Stanković, Slavica

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2016)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Stanković, Slavica
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/645
AB  - Species of the genus Aspergillus, section Flavi are facultative plant pathogens with the capability to contaminate primary agricultural products in the field, at harvest, in warehouses, and during processing. Infection is done through silk or injuries on maize ears. The objective of this study was to test pathogenicity of 25 A. parasiticus isolates on barley leaves. A parasiticus was isolated from maize kernels collected from 22 locations in Serbia during 2013-2015. Inoculation of the first barley leaf was done by the spore suspension of the fungus A. parasiticus. The spot sizes were statistically compared and this comparison showed that the isolate MRIZP 3930 was the least virulent. The isolate MRIZP 3819 was the most virulent seven days after inoculation and together with the isolate MRIZP 4265 was classified into the most aggressive group rated as 5. Pathogenicity was confirmed in all isolates.
AB  - Vrste roda Aspergillus sekcije Flavi su fakultativní biljni patogeni, čija se sposobnost ogleda u kontaminaciji primarnih poljoprivrednih proizvoda u polju, tokom berbe, u skladištu i tokom procesa prerade. Infekcija se obavlja preko svile ili oštećenja na klipu kukuruza. Cilj ovog rada bio je da se oceni patogenost 25 izolata A. parasiticus na listovima ječma. Kulture A. parasiticus su izolovane iz uzoraka zrna kukuruza koji su prikupljani sa 22 lokaliteta u Srbiji u periodu 2013-2015. godina. Inokulacija prvog lista ječma izvršena je pomoću suspenzije spora gljive A. parasiticus. Statističkim poređenjem dobijenih vrednosti veličina pega, pokazano je da je najmanje virulentan izolat MRIZP 3930. Izolat MRIZP 3819 se izvojio kao najvirulentniji posle 7 dana inokulacije, a zajedno s izolatom MRIZP 4265 je svrstan u najagresivniju grupu koja je kategorisana ocenom 5. Svi ispitivani izolati su potvrdili patogenost.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Pathogenicity of Aspergillus spp. isolates originating from Serbia
T1  - Patogenost izolata Aspergillus spp. poreklom iz Srbije
VL  - 53
IS  - 3
SP  - 101
EP  - 105
DO  - 10.5937/ratpov53-10795
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Stanković, Slavica",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/645",
abstract = "Species of the genus Aspergillus, section Flavi are facultative plant pathogens with the capability to contaminate primary agricultural products in the field, at harvest, in warehouses, and during processing. Infection is done through silk or injuries on maize ears. The objective of this study was to test pathogenicity of 25 A. parasiticus isolates on barley leaves. A parasiticus was isolated from maize kernels collected from 22 locations in Serbia during 2013-2015. Inoculation of the first barley leaf was done by the spore suspension of the fungus A. parasiticus. The spot sizes were statistically compared and this comparison showed that the isolate MRIZP 3930 was the least virulent. The isolate MRIZP 3819 was the most virulent seven days after inoculation and together with the isolate MRIZP 4265 was classified into the most aggressive group rated as 5. Pathogenicity was confirmed in all isolates., Vrste roda Aspergillus sekcije Flavi su fakultativní biljni patogeni, čija se sposobnost ogleda u kontaminaciji primarnih poljoprivrednih proizvoda u polju, tokom berbe, u skladištu i tokom procesa prerade. Infekcija se obavlja preko svile ili oštećenja na klipu kukuruza. Cilj ovog rada bio je da se oceni patogenost 25 izolata A. parasiticus na listovima ječma. Kulture A. parasiticus su izolovane iz uzoraka zrna kukuruza koji su prikupljani sa 22 lokaliteta u Srbiji u periodu 2013-2015. godina. Inokulacija prvog lista ječma izvršena je pomoću suspenzije spora gljive A. parasiticus. Statističkim poređenjem dobijenih vrednosti veličina pega, pokazano je da je najmanje virulentan izolat MRIZP 3930. Izolat MRIZP 3819 se izvojio kao najvirulentniji posle 7 dana inokulacije, a zajedno s izolatom MRIZP 4265 je svrstan u najagresivniju grupu koja je kategorisana ocenom 5. Svi ispitivani izolati su potvrdili patogenost.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Pathogenicity of Aspergillus spp. isolates originating from Serbia, Patogenost izolata Aspergillus spp. poreklom iz Srbije",
volume = "53",
number = "3",
pages = "101-105",
doi = "10.5937/ratpov53-10795"
}
Nikolić, M., Savić, I.,& Stanković, S. (2016). Patogenost izolata Aspergillus spp. poreklom iz Srbije.
Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(3), 101-105.
https://doi.org/10.5937/ratpov53-10795
Nikolić M, Savić I, Stanković S. Patogenost izolata Aspergillus spp. poreklom iz Srbije. Ratarstvo i povrtarstvo. 2016;53(3):101-105
Nikolić Milica, Savić Iva, Stanković Slavica, "Patogenost izolata Aspergillus spp. poreklom iz Srbije" Ratarstvo i povrtarstvo, 53, no. 3 (2016):101-105,
https://doi.org/10.5937/ratpov53-10795 .
1

Fungal and mycotoxin contamination of maize hybrids in different maturity groups

Krnjaja, Vesna; Stanković, Slavica; Obradović, Ana; Mandić, Violeta; Bijelić, Zorica; Vasić, Tanja; Jauković, Marko

(Institut za stočarstvo, Beograd, 2016)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Vasić, Tanja
AU  - Jauković, Marko
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/627
AB  - In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods.
AB  - U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fungal and mycotoxin contamination of maize hybrids in different maturity groups
T1  - Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja
VL  - 32
IS  - 1
SP  - 71
EP  - 81
DO  - 10.2298/BAH1601071K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Obradović, Ana and Mandić, Violeta and Bijelić, Zorica and Vasić, Tanja and Jauković, Marko",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/627",
abstract = "In the present study, the frequency of toxigenic fungi and occurrence of aflatoxin B1 (AFB1), deoxynivalenol (DON) and total fumonisins (FBs) in the kernels of six maize hybrids from different FAO maturity groups (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) in three localities (Belosavci, Lađevci and Divci) in Serbia, during the harvest in 2013, was investigated. Using standard mycological tests of maize kernels, the presence of potentially toxigenic fungi species from the genera Aspergillus, Fusarium and Penicillium was found. In the studied localities, species Fusarium verticillioides was the most frequently isolated from the most hybrids, with a maximum frequency of 30%, while the presence of Aspergillus spp. ranged from 0 to 16%, and Penicillium spp. from 0 to 20%. By applying Immunoadsorbent enzymatic assay (ELISA) the concentrations of AFB1, DON and FBs were determined in maize. The differences between tested hybrids in the level of mycotoxins in kernels were statistically significant (P≤0.01) for DON and FBs, but not for the content of AFB1. Also, the interaction between the hybrid and location was significant (P≤0.01) for the level of DON and FBs, while there was no statistical significance for the level of AFB1. Maximum values of AFB1, DON and FBs level were 1.02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) and 1528.56 μg kg-1 (ZP 427). The concentrations of the tested mycotoxins in kernels did not exceed the maximum allowed limits stipulated by Serbian regulations (Službeni glasnik RS, 2014). Given that agro-ecological conditions in Serbia are favourable for the occurrence of toxigenic fungi and their mycotoxins, it is necessary to exert the control of maize kernels annually, in harvest and postharvest periods., U radu je ispitivana učestalost potencijalno toksigenih vrsta gljiva i prirodna pojava aflatoksina B1 (AFB1), deoksinivalenola (DON) i ukupnih fumonizina (FBs) u zrnu šest hibrida kukuruza iz različitih FAO grupa zrenja (ZP 341, ZP 427, ZP 434, ZP 560, ZP 606, ZP 666) u tri lokaliteta (Belosavci, Lađevci i Divci) u Srbiji, tokom berbe u 2013. godini. Primenom standardnih mikoloških ispitivanja zrna kukuruza ustanovljeno je prisustvo potencijalno toksigenih vrsta gljiva iz tri roda: Aspergillus, Fusarium i Penicillium. U ispitivanim lokalitetima, vrsta Fusarium verticillioides je bila najčešće izolovana kod većine hibrida, sa maksimalnom učestalošću od 30%, dok je prisustvo Aspergillus spp. bilo od 0 do 16%, a Penicillium spp. od 0 do 20%. Primenom imunoadsorpcione enzimske metode (ELISA) određen je sadržaj AFB1, DON i FBs u zrnu kukuruza. Razlike između ispitivanih hibrida u sadržaju mikotoksina u zrnu bile su statistički značajne (P≤0,01) za DON i FBs, ali ne i za sadržaj AFB1. Isto tako, interakcija između hibrida i lokaliteta je bila značajna (P≤0,01) za sadržaj DON i FBs, dok nije bilo statističke značajnosti za sadržaj AFB1. Maksimalne koncentracije AFB1, DON i FBs bile su 1,02 μg kg-1 (ZP 427), 12 μg kg-1 (ZP 341) i 1528,56 μg kg-1 (ZP 427). U zrnu kukuruza sadržaj ispitivanih mikotoksina nije premašio maksimalno dozvoljene vrednosti propisane Pravilnikom o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani i hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (Službeni glasnik RS, 2014). S obzirom da su agroekološki uslovi u Srbiji povoljni za pojavu toksigenih gljiva i njihovih mikotoksina, neophodno je svake godine vršiti kontrolu zrna kukuruza, kako u žetvenom, tako i u postžetvenom periodu.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fungal and mycotoxin contamination of maize hybrids in different maturity groups, Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja",
volume = "32",
number = "1",
pages = "71-81",
doi = "10.2298/BAH1601071K"
}
Krnjaja, V., Stanković, S., Obradović, A., Mandić, V., Bijelić, Z., Vasić, T.,& Jauković, M. (2016). Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(1), 71-81.
https://doi.org/10.2298/BAH1601071K
Krnjaja V, Stanković S, Obradović A, Mandić V, Bijelić Z, Vasić T, Jauković M. Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja. Biotechnology in Animal Husbandry. 2016;32(1):71-81
Krnjaja Vesna, Stanković Slavica, Obradović Ana, Mandić Violeta, Bijelić Zorica, Vasić Tanja, Jauković Marko, "Kontaminacija gljivama i mikotoksinima hibrida kukuruza različite grupe zrenja" Biotechnology in Animal Husbandry, 32, no. 1 (2016):71-81,
https://doi.org/10.2298/BAH1601071K .
4

Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain

Tančić, Sonja; Stanković, Slavica; Lević, Jelena; Krnjaja, Vesna

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2015)

TY  - JOUR
AU  - Tančić, Sonja
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Krnjaja, Vesna
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/607
AB  - A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp.
AB  - Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain
T1  - Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza
VL  - 30
IS  - 2
SP  - 99
EP  - 105
DO  - 10.2298/pif1502099T
ER  - 
@article{
author = "Tančić, Sonja and Stanković, Slavica and Lević, Jelena and Krnjaja, Vesna",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/607",
abstract = "A total of 39 Fusarium graminearum, F. sporotrichioides, F. semitectum and F. equiseti isolates, originating from wheat and maize samples collected at 10 locations in Serbia, were analyzed by ELISA method for their potential of deoxynivalenol (DON) and zearalenone (ZEA) production under optimal laboratory conditions. Fusarium graminearum isolates with the highest intraspecies variability were the best producers of both deoxynivalenol and zearalenone. In contrast, F. equiseti isolates were the weakest producers of these two toxins. Considering the plant origin of the isolates, wheat-originating F. sporotrichioides isolates were better deoxynivalenol producers, while the maize-originating isolates produced more zearalenone. There was no clear difference in ZEA production between wheat- and maizeoriginating isolates of F. graminearum, while higher average DON concentrations were produced by F. graminearum wheat-originating isolates. Negative correlation was detected between the production of deoxynivalenol and zearalenone by various Fusarium spp., Potencijal za stvaranje deoksinivalenola (DON) i zearalenona (ZEA) ukupno 39 izolata vrsta Fusarium graminearum, F. sporotrichioides, F. semitectum i F. Equiseti, poreklom iz zrna pšenice i kukuruza sa 10 različitih lokaliteta u Srbiji, analiziran je u optimalnim laboratorijskim uslovima ELISA testom. Izolati vrste F. graminearum su imali najvišu intraspecijsku varijabilnost i ujedno su bili najveći proizvođači i deoksinivalenola i zearalenona. Nasuprot njima, izolati F. equiseti su bili najslabiji proizvođači ova dva mikotoksina. S obzirom na poreklo izolata, F. sporotrichioides izolati poreklom sa pšenice su bili bolji proizvođači deoksinivalenola, dok su izolati ove vrste poreklom sa kukuruza stvarali više koncentracije zearalenona. Među F. graminearum izolatima nije bilo jasne razlike u sintetisanim koncentracijama ZEA zavisno od porekla izolata, dok je pri sintezi DON-a uočena viša prosečna koncentracija sintetisana od strane F. graminearum izolata poreklom sa pšenice. Negativna korelacija je registrovana između sinteze deoksinivalenola i zearalenona od strane Fusarium spp.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Correlation of deoxynivalenol and zearalenone production by Fusarium species originating from wheat and maize grain, Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza",
volume = "30",
number = "2",
pages = "99-105",
doi = "10.2298/pif1502099T"
}
Tančić, S., Stanković, S., Lević, J.,& Krnjaja, V. (2015). Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 30(2), 99-105.
https://doi.org/10.2298/pif1502099T
Tančić S, Stanković S, Lević J, Krnjaja V. Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza. Pesticidi i fitomedicina. 2015;30(2):99-105
Tančić Sonja, Stanković Slavica, Lević Jelena, Krnjaja Vesna, "Korelacija sinteze deoksinivalenola i zearalenola od strane Fusarium izolata poreklom sa zrna pšenice i kukuruza" Pesticidi i fitomedicina, 30, no. 2 (2015):99-105,
https://doi.org/10.2298/pif1502099T .
6

Fusarium infection and deoxynivalenol contamination in winter wheat

Krnjaja, Vesna; Tomić, Z.; Stanković, Slavica; Petrović, T.; Bijelić, Zorica; Mandić, Violeta; Obradović, Ana

(Institut za stočarstvo, Beograd, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Obradović, Ana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/606
AB  - In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.
AB  - U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fusarium infection and deoxynivalenol contamination in winter wheat
T1  - Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice
VL  - 31
IS  - 1
SP  - 123
EP  - 131
DO  - 10.2298/BAH1501123K
ER  - 
@article{
author = "Krnjaja, Vesna and Tomić, Z. and Stanković, Slavica and Petrović, T. and Bijelić, Zorica and Mandić, Violeta and Obradović, Ana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/606",
abstract = "In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat., U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fusarium infection and deoxynivalenol contamination in winter wheat, Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice",
volume = "31",
number = "1",
pages = "123-131",
doi = "10.2298/BAH1501123K"
}
Krnjaja, V., Tomić, Z., Stanković, S., Petrović, T., Bijelić, Z., Mandić, V.,& Obradović, A. (2015). Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 31(1), 123-131.
https://doi.org/10.2298/BAH1501123K
Krnjaja V, Tomić Z, Stanković S, Petrović T, Bijelić Z, Mandić V, Obradović A. Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice. Biotechnology in Animal Husbandry. 2015;31(1):123-131
Krnjaja Vesna, Tomić Z., Stanković Slavica, Petrović T., Bijelić Zorica, Mandić Violeta, Obradović Ana, "Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice" Biotechnology in Animal Husbandry, 31, no. 1 (2015):123-131,
https://doi.org/10.2298/BAH1501123K .
5

Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat

Krnjaja, Vesna; Mandić, Violeta; Lević, Jelena; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Vasić, Tanja

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Vasić, Tanja
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/602
AB  - In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat
VL  - 67
SP  - 251
EP  - 256
DO  - 10.1016/j.cropro.2014.11.001
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Lević, Jelena and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Vasić, Tanja",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/602",
abstract = "In this study, the influence of nitrogen (N)-fertilization on Fusarium head blight (FHB) intensity (disease index), spike weight (SW), grain weight per spike (GWS), fungal incidence and deoxynivalenol (DON) and zearalenone (ZON) contamination was evaluated in winter wheat after artificial inoculation with Fuserium graminearum under field conditions. The trial was conducted using two Serbian wheat cultivars (Pobeda and Renesansa) during two growing seasons (2010-2011 and 2011-2012). Three nitrogen rates, 0, 75 and 150 kg N ha(-1) were evaluated. Nitrogen at higher rates (75 and 150 kg ha(-1)) significantly increased SW and GWS in 2011 but there was no significant impact on FHB index in both growing seasons. ;Cultivars did not differ significantly in SW and GWS; however, FHB index was significantly higher (P = 0.05) in Renesansa in 2012. The effect of N rates was not significant on incidence of F. graminearum and DON and ZON levels in wheat kernels in 2011, but it was significant (P  lt = 0.01) in 2012 in which the highest level of incidence of E graminearum and mycotoxins was observed in the treatment with 150 kg N ha(-1). In both growing seasons, the effects of cultivar and spike treatments were significant (P  lt = 0.01) for DON, whereas these effects were not significant for ZON in 2011. Higher levels of DON were observed in Pobeda, whereas Renesansa had higher levels of ZON. Furthermore, there were higher levels of DON and ZON in inoculated than non-inoculated spikes in both growing seasons. These results indicated that nitrogen fertilization did not increase FHB intensity in both growing seasons and did not increase levels of DON and ZON in 2011. On the other hand, artificial inoculation significantly increased FHB index in both growing seasons, resulting in greater contamination of kernels with E graminearum and mycotoxins, in particular DON. Although the two cultivars differed in their effects on some of the investigated parameters, both were susceptible to FHB and mycotoxin accumulation, in particular DON.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat",
volume = "67",
pages = "251-256",
doi = "10.1016/j.cropro.2014.11.001"
}
Krnjaja, V., Mandić, V., Lević, J., Stanković, S., Obradović, A., Petrović, T.,& Vasić, T. (2015). Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat.
Crop Protection
Elsevier Sci Ltd, Oxford., 67, 251-256.
https://doi.org/10.1016/j.cropro.2014.11.001
Krnjaja V, Mandić V, Lević J, Stanković S, Obradović A, Petrović T, Vasić T. Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat. Crop Protection. 2015;67:251-256
Krnjaja Vesna, Mandić Violeta, Lević Jelena, Stanković Slavica, Obradović Ana, Petrović Tanja, Vasić Tanja, "Influence of N-fertilization on Fusarium head blight and mycotoxin levels in winter wheat" Crop Protection, 67 (2015):251-256,
https://doi.org/10.1016/j.cropro.2014.11.001 .
14
8
11

Presence of deoxynivalenol in winter wheat treated with fungicides

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Mandić, Violeta; Tomić, Z.; Obradović, Ana

(Institut za stočarstvo, Beograd, 2014)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Mandić, Violeta
AU  - Tomić, Z.
AU  - Obradović, Ana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/566
AB  - Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively).
AB  - U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Presence of deoxynivalenol in winter wheat treated with fungicides
T1  - Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima
VL  - 30
IS  - 1
SP  - 167
EP  - 173
DO  - 10.2298/BAH1401167K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Mandić, Violeta and Tomić, Z. and Obradović, Ana",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/566",
abstract = "Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively)., U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Presence of deoxynivalenol in winter wheat treated with fungicides, Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima",
volume = "30",
number = "1",
pages = "167-173",
doi = "10.2298/BAH1401167K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Mandić, V., Tomić, Z.,& Obradović, A. (2014). Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 30(1), 167-173.
https://doi.org/10.2298/BAH1401167K
Krnjaja V, Lević J, Stanković S, Petrović T, Mandić V, Tomić Z, Obradović A. Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima. Biotechnology in Animal Husbandry. 2014;30(1):167-173
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Mandić Violeta, Tomić Z., Obradović Ana, "Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima" Biotechnology in Animal Husbandry, 30, no. 1 (2014):167-173,
https://doi.org/10.2298/BAH1401167K .
1

An outbreak of Aspergillus species in response to environmental conditions in Serbia

Lević, Jelena; Gošić-Dondo, Snežana; Ivanović, Dragica; Stanković, Slavica; Krnjaja, Vesna; Bočarov-Stančić, Aleksandra; Stepanić, Ana

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Gošić-Dondo, Snežana
AU  - Ivanović, Dragica
AU  - Stanković, Slavica
AU  - Krnjaja, Vesna
AU  - Bočarov-Stančić, Aleksandra
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/510
AB  - The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low.
AB  - U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - An outbreak of Aspergillus species in response to environmental conditions in Serbia
T1  - Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji
VL  - 28
IS  - 3
SP  - 167
EP  - 179
DO  - 10.2298/PIF1303167L
ER  - 
@article{
author = "Lević, Jelena and Gošić-Dondo, Snežana and Ivanović, Dragica and Stanković, Slavica and Krnjaja, Vesna and Bočarov-Stančić, Aleksandra and Stepanić, Ana",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/510",
abstract = "The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low., U ovom radu su analizirani učestalost i intenzitet napada A. flavus i A. niger na 180 uzoraka zrna ječma, 1138 kukuruza, 283 soje, 89 suncokreta i 79 pšenice, brojnost leptira kukuruzovog plamenca (Ostrinia nubilalis), kao i njihova interakcija u zavisnosti od meteoroloških uslova u periodu 2008-2012. U agroekološkim uslovima u Srbiji vrsta A . niger je učestalija od vrste A. flavus, a u zavisnosti od biljne vrste pojavljuje se najčešće na zrnu suncokreta, zatim soje, kukuruza, ječma i pšenice. A. flavus je izuzetno bila dominantna na svim biljnim vrstama u 2012, kako po učestalosti, tako i intenzitetu napada. Ova vrsta je u 2012. godini utvrđena kod svih uzoraka soje (100%), nešto manje na zrnu kukuruza (95,3%), zatim na ječmu (65,2%) i suncokretu (57,1%) i najmanje na zrnu pšenice (45,8%). Intenzitet napada A. flavus je, također, bila veća u 2012. u poređenju sa prethodnim godinama. Neuobičajeno visoka učestalost i intenzitet napada A. flavus na zrnu kukuruza u 2012. godini bila je uslovljena izuzetno stresnim agrometeorološkim uslovima, visokim temperaturama i sušom, od cvetanja do voštane zrelosti kukuruza. Kišni faktor (Kf = suma padavina / prosečna mesečna temperatura) ukazuje da je te godine bila izuzetno aridna klima u junu (Kf = 0,57), julu (Kf = 1,45), avgustu (Kf = 0,15) i septembru (Kf = 1,42). Kukuruzov plamenac je drugi činilac koji je uslovio intenzivnu pojavu A. flavus na zrnu kukuruza u 2012. godini. Maksimalni let leptira ovog insekta utvrđen je veoma rano, posebno let druge generacije, već u julu 2012. (5.149 jediniki), a kao posledica toga u vreme berbe su utvrđena i brojna oštećenja na kojima je bio vidljiv razvoj maslinasto-zelenih praškastih kolonija koje su tipične za A. flavus. U hronologji pojave A. flavus ovo su prvi podaci o njenoj jako visokoj učestalosti i intenzitetu napada u agroekološkim uslovima u Srbiji. Intenzivna pojava A. flavus je bila retka u proteklih 50 godina zbog čega je bio i nizak nivo aflatoksina u zrnu kukuruzu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "An outbreak of Aspergillus species in response to environmental conditions in Serbia, Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji",
volume = "28",
number = "3",
pages = "167-179",
doi = "10.2298/PIF1303167L"
}
Lević, J., Gošić-Dondo, S., Ivanović, D., Stanković, S., Krnjaja, V., Bočarov-Stančić, A.,& Stepanić, A. (2013). Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 167-179.
https://doi.org/10.2298/PIF1303167L
Lević J, Gošić-Dondo S, Ivanović D, Stanković S, Krnjaja V, Bočarov-Stančić A, Stepanić A. Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji. Pesticidi i fitomedicina. 2013;28(3):167-179
Lević Jelena, Gošić-Dondo Snežana, Ivanović Dragica, Stanković Slavica, Krnjaja Vesna, Bočarov-Stančić Aleksandra, Stepanić Ana, "Intenzivna pojava vrsta roda Aspergillus kao odgovor na uslove spoljašnje sredine u Srbiji" Pesticidi i fitomedicina, 28, no. 3 (2013):167-179,
https://doi.org/10.2298/PIF1303167L .
29

Detection of Rhizomucor pusillus on sunflower seed

Lević, Jelena; Ivanović, D.; Stanković, Slavica; Milivojević, Marija; Vukadinović, Radmila; Stepanić, Ana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2013)

TY  - JOUR
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Stanković, Slavica
AU  - Milivojević, Marija
AU  - Vukadinović, Radmila
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/478
AB  - The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Detection of Rhizomucor pusillus on sunflower seed
VL  - 36
IS  - 59
SP  - 59
EP  - 70
DO  - 10.2298/HEL1359059L
ER  - 
@article{
author = "Lević, Jelena and Ivanović, D. and Stanković, Slavica and Milivojević, Marija and Vukadinović, Radmila and Stepanić, Ana",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/478",
abstract = "The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Detection of Rhizomucor pusillus on sunflower seed",
volume = "36",
number = "59",
pages = "59-70",
doi = "10.2298/HEL1359059L"
}
Lević, J., Ivanović, D., Stanković, S., Milivojević, M., Vukadinović, R.,& Stepanić, A. (2013). Detection of Rhizomucor pusillus on sunflower seed.
Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 36(59), 59-70.
https://doi.org/10.2298/HEL1359059L
Lević J, Ivanović D, Stanković S, Milivojević M, Vukadinović R, Stepanić A. Detection of Rhizomucor pusillus on sunflower seed. Helia. 2013;36(59):59-70
Lević Jelena, Ivanović D., Stanković Slavica, Milivojević Marija, Vukadinović Radmila, Stepanić Ana, "Detection of Rhizomucor pusillus on sunflower seed" Helia, 36, no. 59 (2013):59-70,
https://doi.org/10.2298/HEL1359059L .

The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Vasić, Tanja

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Vasić, Tanja
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/509
AB  - Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail.
AB  - Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi
T1  - Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva
VL  - 28
IS  - 3
SP  - 157
EP  - 165
DO  - 10.2298/PIF1303157K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Vasić, Tanja",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/509",
abstract = "Visual assessment of phenotypes, performed when two strains of one fungal species are cultivated in a mixed culture on specific media, is known as vegetative or heterokaryotic compatibility or incompatibility test, which enables identification of fungal clones and their classification based on phylogenetic groups. Hyphae of strains that have identical alleles at all vic loci can anastomose into a form of a visible heterokaryon. Strains that divide compatible loci and can anastomose each other belong to a subpopulation termed the vegetative compatibility group (VCG), which is genetically distinguishable from other VCGs. Each VCG is specific regarding its host plant or related host groups and can, but does not have to be virulent on other hosts. Vegetative compatibility can be established in different ways, but complementary auxotrophic strains or strains formed by spontaneous mutation during nutrition, capable of forming a prototrophic heterokaryon are predominantly used. The nit mutants are considered excellent genetic markers for determination of vegetative compatibility and grouping of strains or clones of one fungus into the same or different VCGs. The ability only to determine whether strains are the same or not, but not the degree of their relatedness using VCG, is a limiting factor in analyses that could be performed. VCGs are the most efficient when they are employed to detect the presence of a specific strain in a population. This paper provides an overview of the importance of the phenomenon of vegetative compatibility. Vegetative compatibility is one of the most important genetic traits in ascomycetes by which one subpopulation can be identified as a distinct genetic group. Furthermore, the procedures for isolation, identification and determination of nit mutant phenotypes, and for identification of complementary strains and VCGs are described in detail., Vizuelna ocena fenotipa koja se izvodi kada se dva izolata ili soja jedne vrste gljive gaje u združenim kulturama na specifičnim podlogama, poznata kao test vegetativne ili heterokarionske kompatibilnosti ili nekompatibilnosti, omogućava identifikaciju klonova gljive i njihovu klasifikaciju prema filogenetskim grupama. Hife izolata koje imaju identične alele kod svih vic lokusa mogu da anastomoziraju u oblik vidljivog heterokariona. Izolati koji dele kompatibilne lokuse i mogu da anastomoziraju jedni s drugima pripadaju subpopulaciji označenoj kao vegetativno kompatibilna grupa (VCG) koja je genetski odvojena od drugih vegetativno kompatibilnih grupa (VCGs). Svaka VCG je specifična prema biljci domaćinu ili srodnoj grupi domaćina i može ili ne mora biti virulentna na drugim domaćinima. Vegetativna kompatibilnost može biti utvrđena na različite načine, ali se najčešće koriste komplementarni auksotrofni sojevi, ili sojevi koji su nastali spontanom mutacijom tokom ishrane, a koji su sposobni da obrazuju prototrofni heterokarion. Smatra se da su nit mutanti izvrsni genetički markeri za utvrđivanje vegetativne kompatibilnosti i grupisanje sojeva ili klonova jedne vrste gljive u iste ili različite VCGs. Nesposobnost da se odredi stepen srodnosti korišćenjem VCGs, već samo da li su izolati isti ili ne, ograničava tipove analiza koje bi mogle biti postavljene. VCGs su najefikasnije kada se koriste za utvrđivanje prisustva specifičnog soja u populaciji. U radu je dat pregled značaja fenomena vegetativne kompatibilnosti, kao jedne veoma značajne genetičke osobine kod askomiceta kojom se jedna subpopulacija može identifikovati kao posebna genetička grupa. Isto tako, dat je detaljan opis postupka za izolaciju, identifikaciju i utvrđivanje fenotipa nit mutanata, kao i za identifikaciju komplementarnih sojeva i VCGs.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The use of vegetative compatibility tests for identification of biodiversity of phytopathogenic fungi, Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva",
volume = "28",
number = "3",
pages = "157-165",
doi = "10.2298/PIF1303157K"
}
Krnjaja, V., Lević, J., Stanković, S.,& Vasić, T. (2013). Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva.
Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(3), 157-165.
https://doi.org/10.2298/PIF1303157K
Krnjaja V, Lević J, Stanković S, Vasić T. Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva. Pesticidi i fitomedicina. 2013;28(3):157-165
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Vasić Tanja, "Primena testova vegetativne kompatibilnosti za identifikaciju biodiverziteta fitopatogenih gljiva" Pesticidi i fitomedicina, 28, no. 3 (2013):157-165,
https://doi.org/10.2298/PIF1303157K .
3

Moulds and mycotoxins in stored maize grains

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Tomić, Z.; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Tomić, Z.
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/485
AB  - In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58).
AB  - U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Moulds and mycotoxins in stored maize grains
T1  - Plesni i mikotoksini u uskladištenom kukuruzu
VL  - 29
IS  - 3
SP  - 527
EP  - 536
DO  - 10.2298/BAH1303527K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Tomić, Z. and Mandić, Violeta and Bijelić, Zorica",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/485",
abstract = "In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58)., U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Moulds and mycotoxins in stored maize grains, Plesni i mikotoksini u uskladištenom kukuruzu",
volume = "29",
number = "3",
pages = "527-536",
doi = "10.2298/BAH1303527K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Tomić, Z., Mandić, V.,& Bijelić, Z. (2013). Plesni i mikotoksini u uskladištenom kukuruzu.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(3), 527-536.
https://doi.org/10.2298/BAH1303527K
Krnjaja V, Lević J, Stanković S, Petrović T, Tomić Z, Mandić V, Bijelić Z. Plesni i mikotoksini u uskladištenom kukuruzu. Biotechnology in Animal Husbandry. 2013;29(3):527-536
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Tomić Z., Mandić Violeta, Bijelić Zorica, "Plesni i mikotoksini u uskladištenom kukuruzu" Biotechnology in Animal Husbandry, 29, no. 3 (2013):527-536,
https://doi.org/10.2298/BAH1303527K .
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