Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness

Link to this page

info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//

Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (en)
Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (sr)
Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (sr_RS)
Authors

Publications

Mycobiota and aflatoxin B1 in poultry feeds

Krnjaja, Vesna; Petrović, Tanja; Stanković, Slavica; Lukić, Miloš; Škrbić, Zdenka; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2019)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Petrović, Tanja
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Škrbić, Zdenka
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/742
AB  - In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain.
AB  - U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Mycobiota and aflatoxin B1 in poultry feeds
T1  - Mikobiota i aflatoksin B1 u hrani za živinu
VL  - 35
IS  - 1
SP  - 61
EP  - 69
DO  - 10.2298/BAH1901061K
ER  - 
@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/742",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds, Mikobiota i aflatoksin B1 u hrani za živinu",
volume = "35",
number = "1",
pages = "61-69",
doi = "10.2298/BAH1901061K"
}
Krnjaja, V., Petrović, T., Stanković, S., Lukić, M., Škrbić, Z., Mandić, V.,& Bijelić, Z. (2019). Mikobiota i aflatoksin B1 u hrani za živinu.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 35(1), 61-69.
https://doi.org/10.2298/BAH1901061K
Krnjaja V, Petrović T, Stanković S, Lukić M, Škrbić Z, Mandić V, Bijelić Z. Mikobiota i aflatoksin B1 u hrani za živinu. Biotechnology in Animal Husbandry. 2019;35(1):61-69
Krnjaja Vesna, Petrović Tanja, Stanković Slavica, Lukić Miloš, Škrbić Zdenka, Mandić Violeta, Bijelić Zorica, "Mikobiota i aflatoksin B1 u hrani za živinu" Biotechnology in Animal Husbandry, 35, no. 1 (2019):61-69,
https://doi.org/10.2298/BAH1901061K .

Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia

Krnjaja, Vesna; Stanković, Slavica; Lukić, Miloš; Mićić, Nenad; Petrović, Tanja; Bijelić, Zorica; Mandić, Violeta

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Stanković, Slavica
AU  - Lukić, Miloš
AU  - Mićić, Nenad
AU  - Petrović, Tanja
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/720
AB  - This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.
AB  - Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia
T1  - Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji
VL  - 34
IS  - 2
SP  - 239
EP  - 249
DO  - 10.2298/bah1802239K
ER  - 
@article{
author = "Krnjaja, Vesna and Stanković, Slavica and Lukić, Miloš and Mićić, Nenad and Petrović, Tanja and Bijelić, Zorica and Mandić, Violeta",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/720",
abstract = "This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Šumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Šumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Šumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Šumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Šumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 μg kg-1 ) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain., Ispitivanja u ovom radu izvedena su s ciljem da se odredi prirodna pojava potencijalno toksigenih gljiva iz rodova Aspergillus, Fusarium i Penicillium i sadržaj mikotoksina zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u kukuruzu uskladištenom neposredno posle berbe u 2016. godini i korišćenom za ishranu životinja. Ukupno 22 uzoraka zrna kukuruza sakupljeni su iz četiri regiona u Srbiji: Beogradski (devet uzoraka), Šumadijski (osam uzoraka), Podunavski (četiri uzorka) i Kolubarski (jedan uzorak). Toksigene vrste gljiva su identifikovane na osnovu morfoloških osobina, a sadržaj mikotoksina određen je pomoću biohemijske, imunoadsorpcione enzimske metode (ELISA). Ispitivani uzorci kukuruza većinom su bili inficirani sa Aspergillus, Fusarium i Penicillium spp., izuzev što u uzorku iz Kolubarskog regiona nisu bile identifikovane Aspergillus vrste. Fusarium graminearum bila je najučestalija vrsta u uzorku kukuruza iz Kolubarskog regiona (60%), F. verticillioides u uzorcima iz Podunavskog (43,75%) i Beogradskog regiona (22,4%) i Penicillium spp. u uzorcima iz Šumadijskog regiona (26,38%). U ispitivanim uzorcima kukuruza zastupljenost Aspergillus vrsta bila je niska (0-1,78%). Mikotoksikološkim analizama ustanovljeno je prisustvo zearalenona (ZEA), deoksinivalenola (DON) i aflatoksina B1 (AFB1) u svim ispitivanim uzorcima kukuruza, izuzev što DON nije detektovan u uzorcima iz Podunavskog a AFB1 u uzorku iz Kolubarskog regiona. Ispitivani uzorci su visoko kontaminirani sa ZEA, 100% uzoraka iz Šumadijskog, Podunavskog i Kolubarskog regiona i 88,89% uzoraka iz Beogradskog regiona. Isto tako, sa DON bilo je kontaminirano 100% uzoraka iz Šumadijskog i Kolubarskog regiona i 22,2% iz Beogradskog regiona. Najveći broj AFB1 pozitivnih uzoraka ustanovljen je u Šumadijskom regionu (87,5%), dok je u Beogradskom i Podunavskom regionu ustanovljeno 55,56% i 50% AFB1 pozitivnih uzoraka, respektivno. Uopšteno razmatrajući, u ovim analizama ustanovljena je visoka zastupljenost toksigenih vrsta u svim ispitivanim uzorcima kukuruza. Isto tako, ustanovljena je visoka kontaminiranost uzoraka sa mikotoksinima ZEA, DON i AFB1, iako je samo u jednom uzorku sadržaj DON premašio dozvoljeni limit (1750 μg kg-1 ) prema zakonskoj regulativi za neprerađeni kukuruz. Zbog toga, stalne mikološke i mikotoksikološke analize zrna kukuruza neophodne su radi ocene rizika od gljivične i mikotoksin kontaminacije u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia, Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji",
volume = "34",
number = "2",
pages = "239-249",
doi = "10.2298/bah1802239K"
}
Krnjaja, V., Stanković, S., Lukić, M., Mićić, N., Petrović, T., Bijelić, Z.,& Mandić, V. (2018). Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(2), 239-249.
https://doi.org/10.2298/bah1802239K
Krnjaja V, Stanković S, Lukić M, Mićić N, Petrović T, Bijelić Z, Mandić V. Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji. Biotechnology in Animal Husbandry. 2018;34(2):239-249
Krnjaja Vesna, Stanković Slavica, Lukić Miloš, Mićić Nenad, Petrović Tanja, Bijelić Zorica, Mandić Violeta, "Kontaminacija toksigenim vrstama gljiva i njihovim mikotoksinima uzoraka kukuruza iz različitih regiona u Srbiji" Biotechnology in Animal Husbandry, 34, no. 2 (2018):239-249,
https://doi.org/10.2298/bah1802239K .
1

Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia

Krnjaja, Vesna; Mandić, Violeta; Bijelić, Zorica; Stanković, Slavica; Obradović, Ana; Petrović, Tanja; Bozić, Manja

(Mdpi, Basel, 2018)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
AU  - Stanković, Slavica
AU  - Obradović, Ana
AU  - Petrović, Tanja
AU  - Bozić, Manja
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/702
AB  - Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.
PB  - Mdpi, Basel
T2  - Toxins
T1  - Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia
VL  - 10
IS  - 11
DO  - 10.3390/toxins10110460
ER  - 
@article{
author = "Krnjaja, Vesna and Mandić, Violeta and Bijelić, Zorica and Stanković, Slavica and Obradović, Ana and Petrović, Tanja and Bozić, Manja",
year = "2018",
url = "http://rik.mrizp.rs/handle/123456789/702",
abstract = "Fusarium graminearum as the main causal agent of Fusarium head blight (FHB) and its ability to produce trichothecenes was investigated by molecular techniques. A total of 37 strains isolated from the wheat, harvested in Serbia in 2005, 2008 and 2015, and previously designated by morphological observation as F. graminearum, were used for trichothecene genotypes characterization. The strains were identified using the species-specific primer set FG16R/FG16F while genotypic characterization was done using specific TRI13 and TRI3 sequences of the trichothecene gene clusters. The PCR assays identified all strains as species of F. graminearum sensu stricto with the DON/15-ADON genotype. The quantification of the mycotoxin (DON) was performed using the biochemical assay. The high levels of DON (>20,000 mu g kg(-1)) were recorded in all of the strains from 2005, four strains from 2008 and two strains from 2015. Weather data of the investigated seasons, showed that the optimal temperature, frequent rains and high relative humidity (RH) was very favourable for the development of F. graminearum, affecting the DON biosynthesis.",
publisher = "Mdpi, Basel",
journal = "Toxins",
title = "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia",
volume = "10",
number = "11",
doi = "10.3390/toxins10110460"
}
Krnjaja, V., Mandić, V., Bijelić, Z., Stanković, S., Obradović, A., Petrović, T.,& Bozić, M. (2018). Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia.
Toxins
Mdpi, Basel., 10(11).
https://doi.org/10.3390/toxins10110460
Krnjaja V, Mandić V, Bijelić Z, Stanković S, Obradović A, Petrović T, Bozić M. Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia. Toxins. 2018;10(11)
Krnjaja Vesna, Mandić Violeta, Bijelić Zorica, Stanković Slavica, Obradović Ana, Petrović Tanja, Bozić Manja, "Trichothecene Genotypes of Fusarium graminearum Populations Isolated from Winter Wheat Crops in Serbia" Toxins, 10, no. 11 (2018),
https://doi.org/10.3390/toxins10110460 .
3
1
2

Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder

Kalusević, Ana; Lević, Steva; Čalija, Bojan; Pantić, Milena; Belović, Miona; Pavlović, Vladimir; Bugarski, Branko; Milić, Jela; Žilić, Slađana; Nedović, Viktor

(Taylor & Francis Ltd, Abingdon, 2017)

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Čalija, Bojan
AU  - Pantić, Milena
AU  - Belović, Miona
AU  - Pavlović, Vladimir
AU  - Bugarski, Branko
AU  - Milić, Jela
AU  - Žilić, Slađana
AU  - Nedović, Viktor
PY  - 2017
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/3427
UR  - http://rik.mrizp.rs/handle/123456789/809
AB  - Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder
VL  - 34
IS  - 5
SP  - 475
EP  - 487
DO  - 10.1080/02652048.2017.1354939
ER  - 
@article{
author = "Kalusević, Ana and Lević, Steva and Čalija, Bojan and Pantić, Milena and Belović, Miona and Pavlović, Vladimir and Bugarski, Branko and Milić, Jela and Žilić, Slađana and Nedović, Viktor",
year = "2017",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/3427, http://rik.mrizp.rs/handle/123456789/809",
abstract = "Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder",
volume = "34",
number = "5",
pages = "475-487",
doi = "10.1080/02652048.2017.1354939"
}
Kalusević, A., Lević, S., Čalija, B., Pantić, M., Belović, M., Pavlović, V., Bugarski, B., Milić, J., Žilić, S.,& Nedović, V. (2017). Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder.
Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 34(5), 475-487.
https://doi.org/10.1080/02652048.2017.1354939
Kalusević A, Lević S, Čalija B, Pantić M, Belović M, Pavlović V, Bugarski B, Milić J, Žilić S, Nedović V. Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. Journal of Microencapsulation. 2017;34(5):475-487
Kalusević Ana, Lević Steva, Čalija Bojan, Pantić Milena, Belović Miona, Pavlović Vladimir, Bugarski Branko, Milić Jela, Žilić Slađana, Nedović Viktor, "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder" Journal of Microencapsulation, 34, no. 5 (2017):475-487,
https://doi.org/10.1080/02652048.2017.1354939 .
1
19
14
13

Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder

Kalusević, Ana; Lević, Steva; Pantić, Milena; Pavlović, Vladimir; Nedović, Viktor; Calija, Bojan; Milić, Jela; Belović, Miona; Bugarski, Branko; Žilić, Slađana

(Taylor & Francis Ltd, Abingdon, 2017)

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Nedović, Viktor
AU  - Calija, Bojan
AU  - Milić, Jela
AU  - Belović, Miona
AU  - Bugarski, Branko
AU  - Žilić, Slađana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/695
AB  - Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder
VL  - 34
IS  - 5
SP  - 475
EP  - 487
DO  - 10.1080/02652048.2017.1354939
ER  - 
@article{
author = "Kalusević, Ana and Lević, Steva and Pantić, Milena and Pavlović, Vladimir and Nedović, Viktor and Calija, Bojan and Milić, Jela and Belović, Miona and Bugarski, Branko and Žilić, Slađana",
year = "2017",
url = "http://rik.mrizp.rs/handle/123456789/695",
abstract = "Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder",
volume = "34",
number = "5",
pages = "475-487",
doi = "10.1080/02652048.2017.1354939"
}
Kalusević, A., Lević, S., Pantić, M., Pavlović, V., Nedović, V., Calija, B., Milić, J., Belović, M., Bugarski, B.,& Žilić, S. (2017). Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder.
Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 34(5), 475-487.
https://doi.org/10.1080/02652048.2017.1354939
Kalusević A, Lević S, Pantić M, Pavlović V, Nedović V, Calija B, Milić J, Belović M, Bugarski B, Žilić S. Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. Journal of Microencapsulation. 2017;34(5):475-487
Kalusević Ana, Lević Steva, Pantić Milena, Pavlović Vladimir, Nedović Viktor, Calija Bojan, Milić Jela, Belović Miona, Bugarski Branko, Žilić Slađana, "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder" Journal of Microencapsulation, 34, no. 5 (2017):475-487,
https://doi.org/10.1080/02652048.2017.1354939 .
1
19
14
13

Fusarium infection and deoxynivalenol contamination in winter wheat

Krnjaja, Vesna; Tomić, Z.; Stanković, Slavica; Petrović, T.; Bijelić, Zorica; Mandić, Violeta; Obradović, Ana

(Institut za stočarstvo, Beograd, 2015)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Tomić, Z.
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Bijelić, Zorica
AU  - Mandić, Violeta
AU  - Obradović, Ana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/606
AB  - In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.
AB  - U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Fusarium infection and deoxynivalenol contamination in winter wheat
T1  - Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice
VL  - 31
IS  - 1
SP  - 123
EP  - 131
DO  - 10.2298/BAH1501123K
ER  - 
@article{
author = "Krnjaja, Vesna and Tomić, Z. and Stanković, Slavica and Petrović, T. and Bijelić, Zorica and Mandić, Violeta and Obradović, Ana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/606",
abstract = "In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 μg kg-1 to 1101 μg kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 μg kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat., U radu je proučavana incidenca Fusarium-zaraženih zrna ozime pšenice iz žetve 2014. godine kod dve domaće komercijalne sorte Simonida i NS40S, kao i sadržaj mikotoksina deoksinivalenola (DON). Nivo fuzariozne kontaminacije zrna pšenice određen je primenom fitopatoloških testova standardne metodologije a DON je detektovan primenom imunoadsorpcione enzimske metode (ELISA). Incidenca Fusarium-zaraženih zrna bila je u proseku za sve ispitivane uzorke od 12% (Simonida) do 19% (NS40S). Od Fusarium vrsta identifikovana je jedino Fusarium graminearum, kao dobro poznati producent DON mikotoksina. Pored ove gljivične vrste, u visokom procentu izolovana je Alternaria spp. sa prosečnom incidencom od 53% (Simonida) do 63% (NS40S). U ispitivanim uzorcima pšenice prosečan sadržaj DON bio je od 424 μg kg-1 (Simonida) do 1101 μg kg-1 (NS40S). Između Fusarium-zaraženih zrna i DON utvrđena je statistički neznačajna negativna korelacija (r = - 0.18) kod sorte Simonida i statistički neznačajna pozitivna korelacija (r = 0.11) kod sorte NS40S. Prosečne koncentracije DON u ispitivanim uzorcima pšenice kod obe ispitivane sorte nisu bile iznad maksimalno dozvoljenog limita iako je nivo fuzariozne kontaminacije zrna obe ispitivane sorte bio visok. Ovi rezultati ukazuju da su obe ispitivane sorte pšenice osetljive prema fuzarioznoj infekciji i produkciji DON mikotoksina u agroekološkim uslovima Srbije, s tim što je sorta NS40S osetljivija u odnosu na sortu Simonida. Zbog svega navedenog, redovna zdravstvena kontrola zrna i razvijanje strategije integralnog monitoringa fuzarioze klasa neophodne su preventivne mere borbe u zaštiti pšenice.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Fusarium infection and deoxynivalenol contamination in winter wheat, Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice",
volume = "31",
number = "1",
pages = "123-131",
doi = "10.2298/BAH1501123K"
}
Krnjaja, V., Tomić, Z., Stanković, S., Petrović, T., Bijelić, Z., Mandić, V.,& Obradović, A. (2015). Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 31(1), 123-131.
https://doi.org/10.2298/BAH1501123K
Krnjaja V, Tomić Z, Stanković S, Petrović T, Bijelić Z, Mandić V, Obradović A. Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice. Biotechnology in Animal Husbandry. 2015;31(1):123-131
Krnjaja Vesna, Tomić Z., Stanković Slavica, Petrović T., Bijelić Zorica, Mandić Violeta, Obradović Ana, "Fusarium infekcija i deoksinivalenol kontaminacija ozime pšenice" Biotechnology in Animal Husbandry, 31, no. 1 (2015):123-131,
https://doi.org/10.2298/BAH1501123K .
5

Presence of deoxynivalenol in winter wheat treated with fungicides

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Mandić, Violeta; Tomić, Z.; Obradović, Ana

(Institut za stočarstvo, Beograd, 2014)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Mandić, Violeta
AU  - Tomić, Z.
AU  - Obradović, Ana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/566
AB  - Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively).
AB  - U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Presence of deoxynivalenol in winter wheat treated with fungicides
T1  - Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima
VL  - 30
IS  - 1
SP  - 167
EP  - 173
DO  - 10.2298/BAH1401167K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Mandić, Violeta and Tomić, Z. and Obradović, Ana",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/566",
abstract = "Natural occurrence of Fusarium spp. and concentrations of mycotoxin deoxynivalenol (DON) in the grain of the winter wheat moderately susceptible to Fusarium head blight (FHB) has been studied. Grain samples were collected from wheat crops intended mainly for human consumption. All wheat crops were treated with fungicides before (a.i. flutriafol - formulated as Fluoco, applied in dose of 0.5 l ha-1) and during the flowering phase of growing (a.i. thiophanate-methyl + epoxiconazole formulated as Eskorta plus and a.i. thiophanate-methyl formulated as Funomil, applied in doses of 0.75 and 0.5 l ha-1, respectivily). Among of Fusarium species only F. graminearum, as a well known producer of DON, was identified. This fungus was identified in 15 of 19 samples (78.9%) with incidence in positive samples of 2 to 28% (average, 14.0%). Presence of DON was established in 13 of a total 19 investigated wheat grain samples (68.4%). In positive samples DON was detected in concentrations from 69 to 918 μg kg-1 (average, 478 μg kg-1). DON showed a significant and positive correlation at P≥0.05 with grain moisture content (r = 0.52*). Between the frequency of F. graminearum and concentration of DON and between the frequency of F. graminearum and grain moisture content, positive correlation was determined, but without statistical significance (r = 0.44 and r = 0.29, respectively)., U radu je proučavana prirodna pojava Fusarium spp. i koncentracija mikotoksina deoksinivalenola (DON) u zrnu ozime pšenice srednje osetljive prema fuzariozi klasa (FHB). Uzorci zrna su prikupljeni sa proizvodnih useva pšenice namenjene uglavnom za ljudsku upotrebu. Svi usevi pšenice bili su tretirani sa fungicidima pre (a.m. flutriafol - formulisana kao preparat Fluoco, primenjen u dozi 0,5 l ha-1) i tokom cvetanja biljaka (a.m. tiofanat-metil + epoksikonazol formulisana kao preparat Eskorta plus i a.m. tiofanat-metil formulisana kao Funomil, primenjeni u dozi 0.75 i 0.5 l ha-1, respektivno). Među Fusarium vrstama jedino je identifikovana F. graminearum, koja je poznati producent DON. Ova gljiva je bila identifikovana u 15 od 19 uzoraka (78.9%) sa incidencom od 2 do 28% (prosek 14.0%) u pozitivnim uzorcima. Prisustvo DON je utvrđeno u 13 od ukupno 19 proučavanih uzoraka pšenice (68.4%). U pozitivnim uzorcima DON je detektovan u koncentracijama od 69 do 918 μg kg-1 (prosek 478 μg kg-1). DON je pokazao značajnu i pozitivnu korelaciju pri P≥0.05 sa sadržajem vlage zrna (r = 0.52*). Između učestalosti F. graminearum i koncentracije DON i učestalosti F. graminearum i sadržaja vlage zrna utvrđena je, takođe, pozitivna korelacija ali statistički nije značajna (r = 0.44 i r = 0.29, respektivno).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Presence of deoxynivalenol in winter wheat treated with fungicides, Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima",
volume = "30",
number = "1",
pages = "167-173",
doi = "10.2298/BAH1401167K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Mandić, V., Tomić, Z.,& Obradović, A. (2014). Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 30(1), 167-173.
https://doi.org/10.2298/BAH1401167K
Krnjaja V, Lević J, Stanković S, Petrović T, Mandić V, Tomić Z, Obradović A. Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima. Biotechnology in Animal Husbandry. 2014;30(1):167-173
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Mandić Violeta, Tomić Z., Obradović Ana, "Prisustvo deoksinivalenola u ozimoj pšenici tretiranoj s fungicidima" Biotechnology in Animal Husbandry, 30, no. 1 (2014):167-173,
https://doi.org/10.2298/BAH1401167K .
1

Molds and mycotoxins in freshly harvested maize

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, Tanja; Lukić, Miloš

(Matica srpska, Novi Sad, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, Tanja
AU  - Lukić, Miloš
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/516
AB  - Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02).
AB  - Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Molds and mycotoxins in freshly harvested maize
T1  - Plesni i mikotoksini u zrnu kukuruza posle berbe
IS  - 124
SP  - 111
EP  - 119
DO  - 10.2298/ZMSPN1324111K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, Tanja and Lukić, Miloš",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/516",
abstract = "Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of Aspergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 μg kg-1 for 44.83% of samples, and average concentration higher than 40 μg kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 μg kg-1, while it was 98.38 μg kg-1 and 3590 μg kg-1 for the presence of ZON and FB1, respectively. Moderate positive correlation was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02)., Učestalost toksigenih gljiva (plesni) i koncentracija mikotoksina aflatoksina B1 (AFB1), deoksinivalenola (DON), zearalenona (ZON) i fumonizina B1 (FB1) je proučavana u uzorcima zrna kukuruza prikupljenim odmah posle berbe u 2012. godini. U ispitivanih 29 uzoraka najveću zastupljenost imaju vrste iz rodova Rhizopus (56,41%), Aspergillus (43,66%) i Fusarium (14,97%). Značajno niža učestalost ustanovljena je za vrste roda Penicillium (3.31%), a posebno za vrste iz rodova Alternaria (0,75%) i Cladosporium (0,14%). Među toksigenim vrstama, A. flavus (36,69%) je bila najučestalija vrsta iz roda Aspergillus, dok je F. verticillioides sa prisustvom od 14,69% bila dominantna vrsta roda Fusarium. U svim ispitivanim uzorcima kukuruza ustanovljeno je prisustvo mikotoksina AFB1, ZON-a i FB1 (100%), osim DON-a koji je bio prisutan u 75,86% uzoraka. AFB1 je detektovan u prosečnoj koncentraciji od 13,95 μg kg-1 u 44,83% uzoraka i u prosečnoj koncentraciji od više od 40 μg kg-1 u 55,17% uzoraka. Prosečna koncentracija DON-a je bila 235 μg kg-1, ZON-a 98,38 μg kg-1 i FB1 3590 μg kg-1. Srednja pozitivna korelacija ustanovljena je između koncentracija AFB1 i FB1 (r=0,35), dok je slaba pozitivna korelacija utvrđena između koncentracija ZON-a i DON-a (r=0,02).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Molds and mycotoxins in freshly harvested maize, Plesni i mikotoksini u zrnu kukuruza posle berbe",
number = "124",
pages = "111-119",
doi = "10.2298/ZMSPN1324111K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T.,& Lukić, M. (2013). Plesni i mikotoksini u zrnu kukuruza posle berbe.
Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(124), 111-119.
https://doi.org/10.2298/ZMSPN1324111K
Krnjaja V, Lević J, Stanković S, Petrović T, Lukić M. Plesni i mikotoksini u zrnu kukuruza posle berbe. Zbornik Matice srpske za prirodne nauke. 2013;(124):111-119
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović Tanja, Lukić Miloš, "Plesni i mikotoksini u zrnu kukuruza posle berbe" Zbornik Matice srpske za prirodne nauke, no. 124 (2013):111-119,
https://doi.org/10.2298/ZMSPN1324111K .
2

Moulds and mycotoxins in stored maize grains

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Tomić, Z.; Mandić, Violeta; Bijelić, Zorica

(Institut za stočarstvo, Beograd, 2013)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Tomić, Z.
AU  - Mandić, Violeta
AU  - Bijelić, Zorica
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/485
AB  - In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58).
AB  - U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Moulds and mycotoxins in stored maize grains
T1  - Plesni i mikotoksini u uskladištenom kukuruzu
VL  - 29
IS  - 3
SP  - 527
EP  - 536
DO  - 10.2298/BAH1303527K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Tomić, Z. and Mandić, Violeta and Bijelić, Zorica",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/485",
abstract = "In this study the presence of moulds and mycotoxins in samples of stored maize grains in the period from October 2011 to September 2012 was investigated. Mycological analyses of whole and broken grains showed the presence of species from the genera Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus and others. Among the Aspergillus and Fusarium genera as potentially toxigenic fungi, Aspergillus flavus was identified with the highest percentage on broken grains (20.38%) whereas F. verticilioides was the predominant species in the whole maize grains (34.04%). In addition, it was obtained that tested samples of stored maize grains were 100% positive with aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1) with an average concentration of 1.39 μg kg-1, 71.79 μg kg-1, 128.17 μg kg-1, and 1610.83 μg kg-1, respectively. A significant positive correlation was found between the moisture content and the presence of Fusarium spp. on the broken grains (r = 0.44) and between the moisture content and the concentration of DON (r = 0.61). However, a significant negative correlation was found between moisture content and FB1 (r = -0.34), and between the concentration of ZON and DON mycotoxins (r = -0.58)., U radu je ispitivano prisustvo plesni i mikotoksina u uzorcima zrna uskladištenog kukuruza u periodu od oktobra 2011. do septembra 2012. godine. Mikološkim analizama celog i slomljenog zrna kukuruza ustanovljeno je prisustvo vrsta iz rodova Alternaria, Aspergillus, Fusarium, Penicillium, Rhizopus i drugih. Od potencijalno toksigenih vrsta iz rodova Aspergillus i Fusarium, identifikovane su u najvećem procentu A. flavus na slomljenom (20,38%) i F. verticillioides na celom zrnu kukuruza (34,04%). Ispitivani uzorci uskladištenog kukuruza bili su 100% pozitivni sa aflatoksinom B1 (AFB1), zearalenonom (ZON), deoksinivalenolom (DON) i fumonizinom B1 (FB1) sa prosečnim koncentracijama 1,39 μg kg-1, 71,79 μg kg-1, 128,17 μg kg-1 i 1610,83 μg kg-1, respektivno. Statistički značajna pozitivna korelacija ustanovljena je između sadržaja vlage i prisustva Fusarium spp. na slomljenom zrnu kukuruza (r = 0,44), kao i između sadržaja vlage i koncentracije DON (r = 0,61). Statistički značajna negativna korelacija ustanovljena je između sadržaja vlage i FB1 (r = -0,34), kao i između koncentracija ZON i DON mikotoksina (r = -0,58).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Moulds and mycotoxins in stored maize grains, Plesni i mikotoksini u uskladištenom kukuruzu",
volume = "29",
number = "3",
pages = "527-536",
doi = "10.2298/BAH1303527K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Tomić, Z., Mandić, V.,& Bijelić, Z. (2013). Plesni i mikotoksini u uskladištenom kukuruzu.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(3), 527-536.
https://doi.org/10.2298/BAH1303527K
Krnjaja V, Lević J, Stanković S, Petrović T, Tomić Z, Mandić V, Bijelić Z. Plesni i mikotoksini u uskladištenom kukuruzu. Biotechnology in Animal Husbandry. 2013;29(3):527-536
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Tomić Z., Mandić Violeta, Bijelić Zorica, "Plesni i mikotoksini u uskladištenom kukuruzu" Biotechnology in Animal Husbandry, 29, no. 3 (2013):527-536,
https://doi.org/10.2298/BAH1303527K .
8

Distribution of moulds and mycotoxins in maize grain silage in the trench silo

Krnjaja, Vesna; Lević, Jelena; Stanković, Slavica; Petrović, T.; Stojanović, Lj.; Radović, Čedomir; Gogić, M.

(Institut za stočarstvo, Beograd, 2012)

TY  - JOUR
AU  - Krnjaja, Vesna
AU  - Lević, Jelena
AU  - Stanković, Slavica
AU  - Petrović, T.
AU  - Stojanović, Lj.
AU  - Radović, Čedomir
AU  - Gogić, M.
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/443
AB  - In this study the mycological and mycotoxicological contamination of maize silage samples collected from the bottom (BL), middle (ML) and top (TL) layer in the silage trench silo was studied. Mycological testing of the silage contamination showed the presence of five potential toxigenic fungi of the genera, Aspergillus, Fusarium, Mucor, Penicillium and Rhizopus with their total number ranging from 1 to 10 x 103 CFU g-1. Also, four mycotoxins were detected as followed, aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1). Mycotoxins AFB1 and FB1 were detected as 100% positive in all the samples tested. An average concentration of AFB1 was 2.31 μg kg-1, 2.51 μg kg-1 and 2.81 μg kg-1 in BL, ML and TL respectively, while the concentration of FB1 was 1292.00 μg kg-1, 1298.00 μg kg-1 and 1902.00 μg kg-1 in BL, TL and ML respectively. Average concentration of toxin ZON in positive samples was 15.69 μg kg-1 in BL and 30.68 μg kg-1 in ML, while its presence in the samples taken from the top layer was not detected. An average concentration of DON toxin in positive samples were in the range of 41.71 μg kg-1 for TL, 79.20 μg kg-1 for ML, while it was 97.00 μg kg-1 for BL. There was no statistically significant correlation between moisture content and total number of moulds and concentrations of certain mycotoxins. However, statistically highly significant correlations between the total number of moulds and AFB1 concentration (r = 1.0), ZON (r = 0.99) and FB1 (r = 0.91) were established, while weak correlation was obtained in the case of DON and total mould counts (r = 0.54).
AB  - U radu je ispitivana mikološka i mikotoksikološka kontaminacija uzoraka silaže od zrna kukuruza sakupljenih iz donjeg (DS), srednjeg (SS) i gornjeg (GS) sloja silaže u silo-trenču. Mikološkim ispitivanjima kontaminacije silaže ustanovljeno je prisustvo pet potencijalno toksigenih rodova plesni, Aspergillus, Fusarium, Mucor, Penicillium i Rhizopus sa ukupnim brojem od 1-10 x 103 CFU g- 1. Takođe, detektovana su i četiri mikotoksina kao što su aflatoksin B1 (AFB1), zearalenon (ZON), deoksinivalenol (DON) i fumonizin B1 (FB1). Mikotoksini AFB1 i FB1 detektovani su kao 100% pozitivni u svim ispitivanim uzorcima. Prosečna koncentracija AFB1 bila je 2,31 μg kg-1 za GS, 2,51 μg kg-1 za DS i 2,81 μg kg-1 za SS, dok je prosečna koncentracija FB1 bila 1292,00 μg kg-1 za DS, 1298,00 μg kg-1 za GS i 1902,00 μg kg-1 za SS. Prosečna koncentracija ZON toksina u pozitivnim uzorcima je 15,69 μg kg-1 za DS i 30,68 μg kg-1 za SS, dok njegovo prisustvo nije detektovano u uzorcima iz gornjeg sloja. Prosečna koncentracija DON toksina u pozitivnim uzorcima bila je od 41,71 μg kg-1 za GS, 79,20 μg kg-1 za SS do 97,00 μg kg-1 za DS. Nije utvrđena statistički značajna korelacija sardžaja vlage sa ukupnim brojem plesni i koncentracijom pojedinih mikotoksina. Međutim, utvrđena je statistički veoma značajna korelacija između ukupnog broja plesni i koncentracije AFB1 (r = 1,0), ZON (r = 0,99) i FB1 (r = 0,91), a nešto manja u slučaju DON i ukupnog broja plesni (r = 0,54).
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Distribution of moulds and mycotoxins in maize grain silage in the trench silo
T1  - Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču
VL  - 28
IS  - 4
SP  - 845
EP  - 854
DO  - 10.2298/BAH1204845K
ER  - 
@article{
author = "Krnjaja, Vesna and Lević, Jelena and Stanković, Slavica and Petrović, T. and Stojanović, Lj. and Radović, Čedomir and Gogić, M.",
year = "2012",
url = "http://rik.mrizp.rs/handle/123456789/443",
abstract = "In this study the mycological and mycotoxicological contamination of maize silage samples collected from the bottom (BL), middle (ML) and top (TL) layer in the silage trench silo was studied. Mycological testing of the silage contamination showed the presence of five potential toxigenic fungi of the genera, Aspergillus, Fusarium, Mucor, Penicillium and Rhizopus with their total number ranging from 1 to 10 x 103 CFU g-1. Also, four mycotoxins were detected as followed, aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin B1 (FB1). Mycotoxins AFB1 and FB1 were detected as 100% positive in all the samples tested. An average concentration of AFB1 was 2.31 μg kg-1, 2.51 μg kg-1 and 2.81 μg kg-1 in BL, ML and TL respectively, while the concentration of FB1 was 1292.00 μg kg-1, 1298.00 μg kg-1 and 1902.00 μg kg-1 in BL, TL and ML respectively. Average concentration of toxin ZON in positive samples was 15.69 μg kg-1 in BL and 30.68 μg kg-1 in ML, while its presence in the samples taken from the top layer was not detected. An average concentration of DON toxin in positive samples were in the range of 41.71 μg kg-1 for TL, 79.20 μg kg-1 for ML, while it was 97.00 μg kg-1 for BL. There was no statistically significant correlation between moisture content and total number of moulds and concentrations of certain mycotoxins. However, statistically highly significant correlations between the total number of moulds and AFB1 concentration (r = 1.0), ZON (r = 0.99) and FB1 (r = 0.91) were established, while weak correlation was obtained in the case of DON and total mould counts (r = 0.54)., U radu je ispitivana mikološka i mikotoksikološka kontaminacija uzoraka silaže od zrna kukuruza sakupljenih iz donjeg (DS), srednjeg (SS) i gornjeg (GS) sloja silaže u silo-trenču. Mikološkim ispitivanjima kontaminacije silaže ustanovljeno je prisustvo pet potencijalno toksigenih rodova plesni, Aspergillus, Fusarium, Mucor, Penicillium i Rhizopus sa ukupnim brojem od 1-10 x 103 CFU g- 1. Takođe, detektovana su i četiri mikotoksina kao što su aflatoksin B1 (AFB1), zearalenon (ZON), deoksinivalenol (DON) i fumonizin B1 (FB1). Mikotoksini AFB1 i FB1 detektovani su kao 100% pozitivni u svim ispitivanim uzorcima. Prosečna koncentracija AFB1 bila je 2,31 μg kg-1 za GS, 2,51 μg kg-1 za DS i 2,81 μg kg-1 za SS, dok je prosečna koncentracija FB1 bila 1292,00 μg kg-1 za DS, 1298,00 μg kg-1 za GS i 1902,00 μg kg-1 za SS. Prosečna koncentracija ZON toksina u pozitivnim uzorcima je 15,69 μg kg-1 za DS i 30,68 μg kg-1 za SS, dok njegovo prisustvo nije detektovano u uzorcima iz gornjeg sloja. Prosečna koncentracija DON toksina u pozitivnim uzorcima bila je od 41,71 μg kg-1 za GS, 79,20 μg kg-1 za SS do 97,00 μg kg-1 za DS. Nije utvrđena statistički značajna korelacija sardžaja vlage sa ukupnim brojem plesni i koncentracijom pojedinih mikotoksina. Međutim, utvrđena je statistički veoma značajna korelacija između ukupnog broja plesni i koncentracije AFB1 (r = 1,0), ZON (r = 0,99) i FB1 (r = 0,91), a nešto manja u slučaju DON i ukupnog broja plesni (r = 0,54).",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Distribution of moulds and mycotoxins in maize grain silage in the trench silo, Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču",
volume = "28",
number = "4",
pages = "845-854",
doi = "10.2298/BAH1204845K"
}
Krnjaja, V., Lević, J., Stanković, S., Petrović, T., Stojanović, Lj., Radović, Č.,& Gogić, M. (2012). Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču.
Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 28(4), 845-854.
https://doi.org/10.2298/BAH1204845K
Krnjaja V, Lević J, Stanković S, Petrović T, Stojanović L, Radović Č, Gogić M. Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču. Biotechnology in Animal Husbandry. 2012;28(4):845-854
Krnjaja Vesna, Lević Jelena, Stanković Slavica, Petrović T., Stojanović Lj., Radović Čedomir, Gogić M., "Distribucija plesni i mikotoksina u silaži od zrna kukuruza u silo-trenču" Biotechnology in Animal Husbandry, 28, no. 4 (2012):845-854,
https://doi.org/10.2298/BAH1204845K .
1