The development of technological procedures in forestry with a view to an optimum forest cover realisation

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The development of technological procedures in forestry with a view to an optimum forest cover realisation (en)
Развој технолошких поступака у шумарству у циљу реализације оптималне шумовитости (sr)
Razvoj tehnoloških postupaka u šumarstvu u cilju realizacije optimalne šumovitosti (sr_RS)
Authors

Publications

Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain

Popović, Vladan; Lučić, Aleksandar; Rakonjac, Ljubinko; Milovanović, Jelena; Mladenović Drinić, Snežana; Ristić, Danijela

(Beograd : Društvo genetičara Srbije, 2019)

TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Milovanović, Jelena
AU  - Mladenović Drinić, Snežana
AU  - Ristić, Danijela
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/799
AB  - The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia.
AB  - Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.
PB  - Beograd : Društvo genetičara Srbije
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain
T1  - Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera
VL  - 51
IS  - 3
SP  - 1103
EP  - 1112
DO  - 10.2298/GENSR1903103P
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Rakonjac, Ljubinko and Milovanović, Jelena and Mladenović Drinić, Snežana and Ristić, Danijela",
year = "2019",
abstract = "The process of plant breeding and conservation of gene pool among other things depends on the knowledge of the level of genetic differentiation. The aim of research in this paper was to determine the genetic differentiation of silver fir (Abies albaMill.) populations of regular  type  and  atypical  genotypes  with  pyramidal  crown  that  can  be  found  on  Javor mountain, at  the  site Ogorijevac. The  genetic  differentiation of silver fir  was determined using SSR (Simple Sequence Repeat) markers. Nine SSR pairs of primers gave 29 alleles, while  the  average  number  of  alleles  was  3.2.  The  primer  NFH15  gave  the  smallest number of alleles (two), while the primer SF78 gave the greatest number of alleles (five). Dice  coefficient  of  the  genetic  similarity  was  used  to  obtain  a  dendrogram  by  UPMGA analysis  using  NTSYSpc  statistical  program.  The  genetic  similarity  recorded  among  the individuals P1 and P2 was the largest (0.89), while the populations VI and individual P2 showed the lowest similarity (0.61).Based on the cluster analysis it canbe concluded that the  studied populations and genotypes of silver  fir  with different types of crown are  clearly differentiated. The  basic insight  into  the  level  of  the  genetic  diversity  of  the  natural  populations  of  silver  fir  with the  various types of crown has been provided using selected SSR markers. The obtained results can be used for creating further strategy for the conservation of the available gene pool and the regeneration of silver fir forests in Serbia., Proces oplemenjivanja biljaka i konzervacije genofonda između ostalog, zavisi i od poznavanja stepena  genetičke  diferencijacije.  Cilj  istraživanja  u  ovom  radu  bio  je  da  se  utvrdi  genetička diferencijacija   populacija   jele   (Abies   alba Mill.)normalanog  tipa  i  atipičnih  genotipova piramidalanog tipa krošnje, koje se javljaju na planini Javor, na lokalitetu Ogorijevac. Genetička sličnost odnosno različitost jele određena je upotrebom SSR (Simple Sequence Repeat) markera. Devet SSR pari prajmera dalo je ukupno 29 alela, dok je prosečan broj alela bio 3,2. Prajmer NFH15 dao je najmanji broj alela (dva), dok je prajmer SF78 bio sa najvećim brojem alela (pet). Najviša vrednost koeficijenta genetičke sličnosti utvrđena je između genotipova P1 i P2 (0,89), dok je najniža vrednost koeficijenta genetičke sličnosti utvrđena između populacije VI i genotipa P2 (0,61).Koeficijenti  genetičke  sličnosti  po  Dice,  upotrebljeni  su  za  dobijanje  dendrograma  pomoću UPMGA  analize,  koristeći  NTSYSpc  statistički  program.  Na  osnovu  klaster  analize  može  se zaključiti  da  su  istraživane  populacije  i  genotipovi  jele  sa  različitim  tipom  krošnje  jasno izdiferencirani.  Upotrebom  izabranih  SSR  markera  dat  je  osnovni  uvid  u  nivo  genetičke raznovrsnosti prirodnih populacija jele različitog tipa krošnje. Dobijeni rezultati mogu poslužiti u budućoj strategiji na konzervaciji raspoloživog genofonda i obnavljanju jelovih šuma u Srbiji.",
publisher = "Beograd : Društvo genetičara Srbije, Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain, Procena genetičke diferencijacije jele (Abies alba Mill.) sa Javora pomoću SSR markera",
volume = "51",
number = "3",
pages = "1103-1112",
doi = "10.2298/GENSR1903103P"
}
Popović, V., Lučić, A., Rakonjac, L., Milovanović, J., Mladenović Drinić, S.,& Ristić, D.. (2019). Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika
Beograd : Društvo genetičara Srbije., 51(3), 1103-1112.
https://doi.org/10.2298/GENSR1903103P
Popović V, Lučić A, Rakonjac L, Milovanović J, Mladenović Drinić S, Ristić D. Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain. in Genetika. 2019;51(3):1103-1112.
doi:10.2298/GENSR1903103P .
Popović, Vladan, Lučić, Aleksandar, Rakonjac, Ljubinko, Milovanović, Jelena, Mladenović Drinić, Snežana, Ristić, Danijela, "Application of SSR markers for assessment of genetic differentiation of silver fir (Abies alba Mill.) originating from Javor mountain" in Genetika, 51, no. 3 (2019):1103-1112,
https://doi.org/10.2298/GENSR1903103P . .
1
1
1

Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers

Popović, Vladan; Lučić, Aleksandar; Rakonjac, Ljubinko; Mladenović-Drinić, Snežana; Ristić, Danijela; Cvjetković, Branislav

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Mladenović-Drinić, Snežana
AU  - Ristić, Danijela
AU  - Cvjetković, Branislav
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/659
AB  - The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill.) in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubocica Bare and Stara Planina; Dubocica Bare and Tara) and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara). A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers
VL  - 49
IS  - 3
SP  - 979
EP  - 988
DO  - 10.2298/GENSR1703979P
UR  - conv_970
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Rakonjac, Ljubinko and Mladenović-Drinić, Snežana and Ristić, Danijela and Cvjetković, Branislav",
year = "2017",
abstract = "The paper presents the results of the analysis of genetic variability of eight populations of silver fir (Abies alba Mill.) in Serbia obtained using SSR markers. The genomic DNA was isolated from tissue of needles of all eight populations. Due to the costly and lengthy process a small number of the SSR markers for Abies alba have been developed, so in this study were used the microsatellite markers of related species. The obtained results indicate a low level of the genetic variability between natural populations of silver fir. The total number of alleles detected with nine SSR markers in eight studied populations of silver fir is 28. The range of alleles varies from two for NFF15 to six for SF78 with an average of 3.1 alleles per locus. The mean value of genetic similarity between populations is 0.92. The smallest genetic similarity between pairs of populations is 0.82 (Dubocica Bare and Stara Planina; Dubocica Bare and Tara) and the greatest genetic similarity is 1 (Zlatar and Stara Planina, Zlatar and Tara, Stara Planina and Tara). A basic insight into the level of genetic diversity of natural populations of silver fir in Serbia, which are located in a relatively small area, has been given using a set of SSR markers. The obtained results can be used in the future strategy for the management and regeneration of silver fir forests.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers",
volume = "49",
number = "3",
pages = "979-988",
doi = "10.2298/GENSR1703979P",
url = "conv_970"
}
Popović, V., Lučić, A., Rakonjac, L., Mladenović-Drinić, S., Ristić, D.,& Cvjetković, B.. (2017). Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers. in Genetika
Društvo genetičara Srbije, Beograd., 49(3), 979-988.
https://doi.org/10.2298/GENSR1703979P
conv_970
Popović V, Lučić A, Rakonjac L, Mladenović-Drinić S, Ristić D, Cvjetković B. Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers. in Genetika. 2017;49(3):979-988.
doi:10.2298/GENSR1703979P
conv_970 .
Popović, Vladan, Lučić, Aleksandar, Rakonjac, Ljubinko, Mladenović-Drinić, Snežana, Ristić, Danijela, Cvjetković, Branislav, "Assessment of genetic diversity of silver fir (Abies alba mill.) In Serbia using SSR markers" in Genetika, 49, no. 3 (2017):979-988,
https://doi.org/10.2298/GENSR1703979P .,
conv_970 .
1
3
3

Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers

Popović, Vladan; Rakonjac, Ljubinko; Lučić, Aleksandar; Ristić, Danijela; Mladenović-Drinić, Snežana; Hadrović, Sabahudin

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Popović, Vladan
AU  - Rakonjac, Ljubinko
AU  - Lučić, Aleksandar
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
AU  - Hadrović, Sabahudin
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/593
AB  - The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of detected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers
VL  - 47
IS  - 2
SP  - 571
EP  - 580
DO  - 10.2298/GENSR1502571P
UR  - conv_917
ER  - 
@article{
author = "Popović, Vladan and Rakonjac, Ljubinko and Lučić, Aleksandar and Ristić, Danijela and Mladenović-Drinić, Snežana and Hadrović, Sabahudin",
year = "2015",
abstract = "The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of detected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers",
volume = "47",
number = "2",
pages = "571-580",
doi = "10.2298/GENSR1502571P",
url = "conv_917"
}
Popović, V., Rakonjac, L., Lučić, A., Ristić, D., Mladenović-Drinić, S.,& Hadrović, S.. (2015). Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers. in Genetika
Društvo genetičara Srbije, Beograd., 47(2), 571-580.
https://doi.org/10.2298/GENSR1502571P
conv_917
Popović V, Rakonjac L, Lučić A, Ristić D, Mladenović-Drinić S, Hadrović S. Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers. in Genetika. 2015;47(2):571-580.
doi:10.2298/GENSR1502571P
conv_917 .
Popović, Vladan, Rakonjac, Ljubinko, Lučić, Aleksandar, Ristić, Danijela, Mladenović-Drinić, Snežana, Hadrović, Sabahudin, "Analysis of intra-population variability of bald cypress (Taxodium distichum l. Rich.) In seed stand near Backa Palanka using RAPD markers" in Genetika, 47, no. 2 (2015):571-580,
https://doi.org/10.2298/GENSR1502571P .,
conv_917 .
1
2
2

Analysis of inter-line variability of bald cypress (Taxodium distichum l. Rich.) Juvenile seedlings using morphometric markers

Popović, Vladan; Lučić, Aleksandar; Ćirković-Mitrović, Tatjana; Rakonjac, Ljubinko; Šijačić-Nikolić, Mirjana; Cvjetković, Branislav; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2014)

TY  - JOUR
AU  - Popović, Vladan
AU  - Lučić, Aleksandar
AU  - Ćirković-Mitrović, Tatjana
AU  - Rakonjac, Ljubinko
AU  - Šijačić-Nikolić, Mirjana
AU  - Cvjetković, Branislav
AU  - Mladenović-Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/544
AB  - In this paper are presented results of the analysis of inter-line variability of morphometric characters of Bald cypress juvenile seedlings at the level of 20 half-sib lines. Obtained results contribute to better understanding of relationship of analyzed characters, as well as the influence of analyzed characters to the differentiation of half-sib lines. Based on these results, differences were observed in the values of the analyzed characters for each half-sib line individually. The determined values of seedlings' size indicate good genetic and adaptive potential of this species, which can be considered as a starting point for the mass production of quality planting material and its use in Serbia.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Analysis of inter-line variability of bald cypress (Taxodium distichum l. Rich.) Juvenile seedlings using morphometric markers
VL  - 46
IS  - 1
SP  - 117
EP  - 128
DO  - 10.2298/GENSR1401117P
UR  - conv_882
ER  - 
@article{
author = "Popović, Vladan and Lučić, Aleksandar and Ćirković-Mitrović, Tatjana and Rakonjac, Ljubinko and Šijačić-Nikolić, Mirjana and Cvjetković, Branislav and Mladenović-Drinić, Snežana",
year = "2014",
abstract = "In this paper are presented results of the analysis of inter-line variability of morphometric characters of Bald cypress juvenile seedlings at the level of 20 half-sib lines. Obtained results contribute to better understanding of relationship of analyzed characters, as well as the influence of analyzed characters to the differentiation of half-sib lines. Based on these results, differences were observed in the values of the analyzed characters for each half-sib line individually. The determined values of seedlings' size indicate good genetic and adaptive potential of this species, which can be considered as a starting point for the mass production of quality planting material and its use in Serbia.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Analysis of inter-line variability of bald cypress (Taxodium distichum l. Rich.) Juvenile seedlings using morphometric markers",
volume = "46",
number = "1",
pages = "117-128",
doi = "10.2298/GENSR1401117P",
url = "conv_882"
}
Popović, V., Lučić, A., Ćirković-Mitrović, T., Rakonjac, L., Šijačić-Nikolić, M., Cvjetković, B.,& Mladenović-Drinić, S.. (2014). Analysis of inter-line variability of bald cypress (Taxodium distichum l. Rich.) Juvenile seedlings using morphometric markers. in Genetika
Društvo genetičara Srbije, Beograd., 46(1), 117-128.
https://doi.org/10.2298/GENSR1401117P
conv_882
Popović V, Lučić A, Ćirković-Mitrović T, Rakonjac L, Šijačić-Nikolić M, Cvjetković B, Mladenović-Drinić S. Analysis of inter-line variability of bald cypress (Taxodium distichum l. Rich.) Juvenile seedlings using morphometric markers. in Genetika. 2014;46(1):117-128.
doi:10.2298/GENSR1401117P
conv_882 .
Popović, Vladan, Lučić, Aleksandar, Ćirković-Mitrović, Tatjana, Rakonjac, Ljubinko, Šijačić-Nikolić, Mirjana, Cvjetković, Branislav, Mladenović-Drinić, Snežana, "Analysis of inter-line variability of bald cypress (Taxodium distichum l. Rich.) Juvenile seedlings using morphometric markers" in Genetika, 46, no. 1 (2014):117-128,
https://doi.org/10.2298/GENSR1401117P .,
conv_882 .
7
2
2

Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers

Lučić, Aleksandar; Popović, Vladan; Rakonjac, Ljubinko; Ćirković-Mitrović, Tatjana; Nevenić, Marija; Ristić, Danijela; Mladenović-Drinić, Snežana

(Srpsko biološko društvo, Beograd, i dr., 2014)

TY  - JOUR
AU  - Lučić, Aleksandar
AU  - Popović, Vladan
AU  - Rakonjac, Ljubinko
AU  - Ćirković-Mitrović, Tatjana
AU  - Nevenić, Marija
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/537
AB  - This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mu g/mu l. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i. e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers
VL  - 66
IS  - 4
SP  - 1485
EP  - 1492
DO  - 10.2298/ABS1404485L
UR  - conv_891
ER  - 
@article{
author = "Lučić, Aleksandar and Popović, Vladan and Rakonjac, Ljubinko and Ćirković-Mitrović, Tatjana and Nevenić, Marija and Ristić, Danijela and Mladenović-Drinić, Snežana",
year = "2014",
abstract = "This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mu g/mu l. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i. e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers",
volume = "66",
number = "4",
pages = "1485-1492",
doi = "10.2298/ABS1404485L",
url = "conv_891"
}
Lučić, A., Popović, V., Rakonjac, L., Ćirković-Mitrović, T., Nevenić, M., Ristić, D.,& Mladenović-Drinić, S.. (2014). Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(4), 1485-1492.
https://doi.org/10.2298/ABS1404485L
conv_891
Lučić A, Popović V, Rakonjac L, Ćirković-Mitrović T, Nevenić M, Ristić D, Mladenović-Drinić S. Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers. in Archives of Biological Sciences. 2014;66(4):1485-1492.
doi:10.2298/ABS1404485L
conv_891 .
Lučić, Aleksandar, Popović, Vladan, Rakonjac, Ljubinko, Ćirković-Mitrović, Tatjana, Nevenić, Marija, Ristić, Danijela, Mladenović-Drinić, Snežana, "Genetic diversity of scots pine (Pinus sylvestris l.) Populations in Serbia revealed by SSR markers" in Archives of Biological Sciences, 66, no. 4 (2014):1485-1492,
https://doi.org/10.2298/ABS1404485L .,
conv_891 .
1
4
5

Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia

Lučić, Aleksandar; Cvjetićanin, Rade; Novaković-Vuković, Marijana; Ristić, Danijela; Popović, Vladan; Rakonjac, Ljubinko; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2013)

TY  - JOUR
AU  - Lučić, Aleksandar
AU  - Cvjetićanin, Rade
AU  - Novaković-Vuković, Marijana
AU  - Ristić, Danijela
AU  - Popović, Vladan
AU  - Rakonjac, Ljubinko
AU  - Mladenović-Drinić, Snežana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/515
AB  - Multidisciplinary studies (genetic and phytocoenological) of 4 populations of Austrian pine (Pinus nigra Arnold) were carried out in western and central Serbia. The obtained results gave the same inter-population arrangement in both methods. Dendograms (NTSYS) differentiate at the greatest genetic distance the population of Austrian pine in Šargan compared to other populations: Crni Vrh, Goč and Studenica. Using phytocoenological analysis it was determined that population of Austrian pine in Šargan forms a community Erico-Pinetum gocensis, while the other populations form a community Seslerio rigidae-Pinetum gocensis. Multidisciplinary approach that was demonstrated within this paper presents the first studies of Austrian pine that directly link genotype dependence and environmental conditions manifested through the phytocoenological affiliation.
AB  - Multidisciplinarni pristup demostriran u okviru ovog rada predstavlja prva istraživanja na crnom boru koje u direktnu vezu dovodi zavisnost genotipa i uslove sredine ispoljene kroz fitocenološku pripadnost. U radu su izvršena multidisciplinarna istraživanja (genetička i fitocenološka) 4 populacije crnog bora (Pinus nigra Arnold) u zapadnoj i centralnoj Srbiji. Izvršena je analiza PCR amplifikacija genomske DNA sa 14 RAPD primera, od čega je 10 polimorfno. Korišćene su tri vrste koeficienata genetičke sličnosti, Jaccard, Dice and Socal and Michener. Dobijeni dendogrami (NTSYS) diferenciraju na najvećoj genetičkoj distanci populaciju crnog bora na Šarganu u odnosu na ostale populacije Crni Vrh, Goč i Studenica. Fitocenološke analize su vršene po metodi Braun-Blanquet i tom prilikom je utvrđeno da populacija crnog bora na Šarganu gradi zajednicu crnog bora sa crnjušom (Erico-Pinetum gocensis), a ostale populacije formiraju zajednice crnog bora sa uskolisnom šašikom (Seslerio rigidae-Pinetum gocensis). Dobijenih rezultata korišćenjem genetičke analize (PCR) i analize fitocenološke pripadnosti dali su isti međupopulacioni raspored.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia
T1  - Međupopulaciona varijabilnost crnog bora (Pinus nigra Arnold) u Srbiji
VL  - 45
IS  - 3
SP  - 641
EP  - 654
DO  - 10.2298/GENSR1303641L
UR  - conv_483
ER  - 
@article{
author = "Lučić, Aleksandar and Cvjetićanin, Rade and Novaković-Vuković, Marijana and Ristić, Danijela and Popović, Vladan and Rakonjac, Ljubinko and Mladenović-Drinić, Snežana",
year = "2013",
abstract = "Multidisciplinary studies (genetic and phytocoenological) of 4 populations of Austrian pine (Pinus nigra Arnold) were carried out in western and central Serbia. The obtained results gave the same inter-population arrangement in both methods. Dendograms (NTSYS) differentiate at the greatest genetic distance the population of Austrian pine in Šargan compared to other populations: Crni Vrh, Goč and Studenica. Using phytocoenological analysis it was determined that population of Austrian pine in Šargan forms a community Erico-Pinetum gocensis, while the other populations form a community Seslerio rigidae-Pinetum gocensis. Multidisciplinary approach that was demonstrated within this paper presents the first studies of Austrian pine that directly link genotype dependence and environmental conditions manifested through the phytocoenological affiliation., Multidisciplinarni pristup demostriran u okviru ovog rada predstavlja prva istraživanja na crnom boru koje u direktnu vezu dovodi zavisnost genotipa i uslove sredine ispoljene kroz fitocenološku pripadnost. U radu su izvršena multidisciplinarna istraživanja (genetička i fitocenološka) 4 populacije crnog bora (Pinus nigra Arnold) u zapadnoj i centralnoj Srbiji. Izvršena je analiza PCR amplifikacija genomske DNA sa 14 RAPD primera, od čega je 10 polimorfno. Korišćene su tri vrste koeficienata genetičke sličnosti, Jaccard, Dice and Socal and Michener. Dobijeni dendogrami (NTSYS) diferenciraju na najvećoj genetičkoj distanci populaciju crnog bora na Šarganu u odnosu na ostale populacije Crni Vrh, Goč i Studenica. Fitocenološke analize su vršene po metodi Braun-Blanquet i tom prilikom je utvrđeno da populacija crnog bora na Šarganu gradi zajednicu crnog bora sa crnjušom (Erico-Pinetum gocensis), a ostale populacije formiraju zajednice crnog bora sa uskolisnom šašikom (Seslerio rigidae-Pinetum gocensis). Dobijenih rezultata korišćenjem genetičke analize (PCR) i analize fitocenološke pripadnosti dali su isti međupopulacioni raspored.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia, Međupopulaciona varijabilnost crnog bora (Pinus nigra Arnold) u Srbiji",
volume = "45",
number = "3",
pages = "641-654",
doi = "10.2298/GENSR1303641L",
url = "conv_483"
}
Lučić, A., Cvjetićanin, R., Novaković-Vuković, M., Ristić, D., Popović, V., Rakonjac, L.,& Mladenović-Drinić, S.. (2013). Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 45(3), 641-654.
https://doi.org/10.2298/GENSR1303641L
conv_483
Lučić A, Cvjetićanin R, Novaković-Vuković M, Ristić D, Popović V, Rakonjac L, Mladenović-Drinić S. Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia. in Genetika. 2013;45(3):641-654.
doi:10.2298/GENSR1303641L
conv_483 .
Lučić, Aleksandar, Cvjetićanin, Rade, Novaković-Vuković, Marijana, Ristić, Danijela, Popović, Vladan, Rakonjac, Ljubinko, Mladenović-Drinić, Snežana, "Interpopulation variability of Austrian pine (Pinus nigra Arnold) in Serbia" in Genetika, 45, no. 3 (2013):641-654,
https://doi.org/10.2298/GENSR1303641L .,
conv_483 .
1
3
2

Interpopulation genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia

Lučić, Aleksandar; Isajev, Vasilije; Cvjetičanin, Rade; Rakonjac, Ljubinko; Novaković, Marijana; Nikolić, Ana; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2011)

TY  - JOUR
AU  - Lučić, Aleksandar
AU  - Isajev, Vasilije
AU  - Cvjetičanin, Rade
AU  - Rakonjac, Ljubinko
AU  - Novaković, Marijana
AU  - Nikolić, Ana
AU  - Mladenović-Drinić, Snežana
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/369
AB  - The genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia was studied in the populations at five localities in western and south-western Serbia. Three groups of Scots pine (Pinus sylvestris L.) populations were differentiated based on genetic research (seed protein analysis) and plant community research. The first group consists of Scots pine populations on Šargan (FMU 'Šargan') and on Tara (FMU 'Kaluderske Bare'), where the forests belong to the community of Scots pine and Austrian pine (Pinetum sylvestris-nigrae Pavlovic 1951). The second group covers the localities Stolovi (FMU 'Radocelo-Crepuljnik') and Zlatar (FMU 'Zlatar I'), where the forests belong to the community of Scots pine and spruce (Piceo abietis-Pinetum sylvestris Stefanovic 1960). The third group comprises the Scots pine population on Pešter (FMU 'Dubocica-Bare') which belongs to the community of Scots pine with erica (Erico-Pinetum sylvestris Stefanovic 1963). Cluster analysis was performed on the basis of seed protein data and showed that there are three groups of Scots pine populations. The three populations coincide with plant communities. The community of Scots pine with erica (Erico-Pinetum sylvestris Stefanovic 1963) recorded on Pešter at the locality 'Dubocica- Bare' in the area of FE 'Golija' Ivanjica, is a special Scots pine population displayed at the greatest distance from all other populations in the cluster analysis dendrogram.
AB  - U radu su predstavljeni rezultati proučavanja genetičko-ekološke varijabilnosti belog bora (Pinus sylvestris L.) u Srbiji. Za istraživanja su izabrane populacije na 5 lokaliteta u zapadnoj i jugozapadnoj Srbiji. Na osnovu genetičkih (analizom proteina u semenu) i fitocenoloških istraživanja izdiferencirane su tri grupe populacija belog bora (Pinus sylvestris L.). Prvu grupu populacija čini beli bor na Šarganu (G.J. 'Šargan') i na Tari (G.J. Kaluđerske bare) čije šume fitocenološki pripadaju zajednici belog i crnog bora (Pinetum sylvestris-nigrae Pavlovic 1951). Drugu grupu čine lokaliteti Stolovi (G.J. 'Radocelo-Crepuljnik') i Zlatar (G.J. 'Zlatar I') koji fitocenološki pripadaju zajednici belog bora i smrče (Piceo abietis- Pinetum sylvestris Stefanovic 1960).Treću grupu čine populacije belog bora na Pešteru (G.J.'Dubocica-Bare') koja fitocenološki pripada zajednici belog bora sa crnjušom (Erico-Pinetum sylvestris Stefanovic 1963). Na osnovu dendrograma klaster analize koji je urađen prema sadržaju proteina u semenu, ustanovljene su tri grupe populacija belog bora. Te populacije se poklapaju sa fitocenozama tako da zajednica belog bora sa crnjušom (Erico-Pinetum sylvestris Stefanović 1963) ustanovljena je na Pešteru, na lokalitetu 'Dubocica-Bare' i na području Š.G. 'Golija' Ivanjica predstavlja posebnu populaciju belog bora koja se na dendrogramu klaster analize odvaja na najvećoj distanci od svih ostalih populacija. Proučavanja međupopulacione genetičko-ekološke varijabilnost belog bora (Pinus sylvestris L.) u Srbiji, obavljena su multidisciplinarno, primenom molekularnih markera i fitocenološkim analizama. Primenjene metoda ispitivanja, omogućile su bolje upoznavanje genetičko-ekoloških karakteristika populacija belog bora u delu areala na prostoru Srbije, što je osnova za dalje usmereno korišćenje ukupnog biološkog potencijala vrste.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Interpopulation genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia
T1  - Međupopulaciona genetičko-ekološka varijabilnost belog bora (Pinus sylvestris L.) u Srbiji
VL  - 43
IS  - 1
SP  - 1
EP  - 18
DO  - 10.2298/GENSR1101001L
UR  - conv_451
ER  - 
@article{
author = "Lučić, Aleksandar and Isajev, Vasilije and Cvjetičanin, Rade and Rakonjac, Ljubinko and Novaković, Marijana and Nikolić, Ana and Mladenović-Drinić, Snežana",
year = "2011",
abstract = "The genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia was studied in the populations at five localities in western and south-western Serbia. Three groups of Scots pine (Pinus sylvestris L.) populations were differentiated based on genetic research (seed protein analysis) and plant community research. The first group consists of Scots pine populations on Šargan (FMU 'Šargan') and on Tara (FMU 'Kaluderske Bare'), where the forests belong to the community of Scots pine and Austrian pine (Pinetum sylvestris-nigrae Pavlovic 1951). The second group covers the localities Stolovi (FMU 'Radocelo-Crepuljnik') and Zlatar (FMU 'Zlatar I'), where the forests belong to the community of Scots pine and spruce (Piceo abietis-Pinetum sylvestris Stefanovic 1960). The third group comprises the Scots pine population on Pešter (FMU 'Dubocica-Bare') which belongs to the community of Scots pine with erica (Erico-Pinetum sylvestris Stefanovic 1963). Cluster analysis was performed on the basis of seed protein data and showed that there are three groups of Scots pine populations. The three populations coincide with plant communities. The community of Scots pine with erica (Erico-Pinetum sylvestris Stefanovic 1963) recorded on Pešter at the locality 'Dubocica- Bare' in the area of FE 'Golija' Ivanjica, is a special Scots pine population displayed at the greatest distance from all other populations in the cluster analysis dendrogram., U radu su predstavljeni rezultati proučavanja genetičko-ekološke varijabilnosti belog bora (Pinus sylvestris L.) u Srbiji. Za istraživanja su izabrane populacije na 5 lokaliteta u zapadnoj i jugozapadnoj Srbiji. Na osnovu genetičkih (analizom proteina u semenu) i fitocenoloških istraživanja izdiferencirane su tri grupe populacija belog bora (Pinus sylvestris L.). Prvu grupu populacija čini beli bor na Šarganu (G.J. 'Šargan') i na Tari (G.J. Kaluđerske bare) čije šume fitocenološki pripadaju zajednici belog i crnog bora (Pinetum sylvestris-nigrae Pavlovic 1951). Drugu grupu čine lokaliteti Stolovi (G.J. 'Radocelo-Crepuljnik') i Zlatar (G.J. 'Zlatar I') koji fitocenološki pripadaju zajednici belog bora i smrče (Piceo abietis- Pinetum sylvestris Stefanovic 1960).Treću grupu čine populacije belog bora na Pešteru (G.J.'Dubocica-Bare') koja fitocenološki pripada zajednici belog bora sa crnjušom (Erico-Pinetum sylvestris Stefanovic 1963). Na osnovu dendrograma klaster analize koji je urađen prema sadržaju proteina u semenu, ustanovljene su tri grupe populacija belog bora. Te populacije se poklapaju sa fitocenozama tako da zajednica belog bora sa crnjušom (Erico-Pinetum sylvestris Stefanović 1963) ustanovljena je na Pešteru, na lokalitetu 'Dubocica-Bare' i na području Š.G. 'Golija' Ivanjica predstavlja posebnu populaciju belog bora koja se na dendrogramu klaster analize odvaja na najvećoj distanci od svih ostalih populacija. Proučavanja međupopulacione genetičko-ekološke varijabilnost belog bora (Pinus sylvestris L.) u Srbiji, obavljena su multidisciplinarno, primenom molekularnih markera i fitocenološkim analizama. Primenjene metoda ispitivanja, omogućile su bolje upoznavanje genetičko-ekoloških karakteristika populacija belog bora u delu areala na prostoru Srbije, što je osnova za dalje usmereno korišćenje ukupnog biološkog potencijala vrste.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Interpopulation genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia, Međupopulaciona genetičko-ekološka varijabilnost belog bora (Pinus sylvestris L.) u Srbiji",
volume = "43",
number = "1",
pages = "1-18",
doi = "10.2298/GENSR1101001L",
url = "conv_451"
}
Lučić, A., Isajev, V., Cvjetičanin, R., Rakonjac, L., Novaković, M., Nikolić, A.,& Mladenović-Drinić, S.. (2011). Interpopulation genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 43(1), 1-18.
https://doi.org/10.2298/GENSR1101001L
conv_451
Lučić A, Isajev V, Cvjetičanin R, Rakonjac L, Novaković M, Nikolić A, Mladenović-Drinić S. Interpopulation genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia. in Genetika. 2011;43(1):1-18.
doi:10.2298/GENSR1101001L
conv_451 .
Lučić, Aleksandar, Isajev, Vasilije, Cvjetičanin, Rade, Rakonjac, Ljubinko, Novaković, Marijana, Nikolić, Ana, Mladenović-Drinić, Snežana, "Interpopulation genetic-ecological variation of Scots pine (Pinus sylvestris L.) in Serbia" in Genetika, 43, no. 1 (2011):1-18,
https://doi.org/10.2298/GENSR1101001L .,
conv_451 .
1
6
5