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Molecular and morphological detection of globodera rostochiensis (nematoda: Heteroderidae) in a seed potato crop

Oro, Violeta; Stanisavljević, Rade; Tabaković, Marijenka; Djokić, Dragoslav

(Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi, 2023)

TY  - CONF
AU  - Oro, Violeta
AU  - Stanisavljević, Rade
AU  - Tabaković, Marijenka
AU  - Djokić, Dragoslav
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1167
AB  - Potato (Solanum tuberosum L.) is one of four major food crops in the world beside wheat, maize and rice. The plant
originated in the highlands of Peru particularly the region around Lake Titicaca and it was first domesticated at least 7
000 years ago. The food security provided by potato and maize allowed the development and survival of civilizations
such as, Huari and Inca for centuries. In the 16th century the Spanish conquistadores searching for the "treasure of the
Andes" brought to Europe, beside gold, potato along with its parasites – the potato cyst nematodes (PCN): Globodera
rostochiensis (Wollenweber) Behrens and G. pallida (Stone) Behrens, two nematode species that have quarantine status.
The morphology of potato cyst nematodes was until recently almost the only way to identify these quarantine organisms.
In the last two decades, molecular analyses as new trends in modern agriculture, contributed to faster and more efficient
identification of these species and allowed insight into the genetic structure of those parts that were practically inaccessible
by morphological studies. The nematodes are present in all European potato growing regions, especially in the Balkan
(Helm) peninsula, either PCN or both are reported.
The collected specimens of cysts were found in soil originating from a seed potato crop in a village near Gornji Milanovac
after the official phytosanitary control in 2022. Individual cysts were used for DNA extraction with a Dneasy blood &
tissue kit. The PCR was done with primers for direct sequencing: TW81 and AB28. The ITS1-5.8S-ITS2 region of PCN
is used for confirmation of species identity together with its morphological characterization. According to EPPO
Standards, the morphological identification comprised larval and cyst characteristics, namely larval stylet length and stylet
knob shape, cyst vulval basin diameter, distance between vulva and anus, Granek´s ratio, and number of cuticular ridges
in perineal area.
Results confirmed the species identity. The morphology of our population of G. rostochiensis was similar to the previously
reported domestic and foreign populations. The degree of similarity was expressed as a percentage of direct matching i.e.
pairwise distances. Phylogenetic analyses indicated a possible ancestor of our PCN population showing evolutionary
relationships among world populations of G. rostochiensis and a phylogenetic placement of the Serbian population.
AB  - Krompir (Solanum tuberosum L.) je jedan od četiri glavna prehrambena useva u svetu pored pšenice, kukuruza i pirinča.
Biljka potiče iz planinskog pojasa Perua, posebno iz regiona oko jezera Titikaka i prvi put je odomaćena pre najmanje 7
000 godina. Sigurnost u hrani koja je nastala gajenjem krompira i kukuruza je omogućila vekovni razvoj i opstanak
civilizacija kao što su Huari i Inke. U 16. veku su španski osvajači tražeći "blago sa Anda" pored zlata, u Evropu doneli i
krompir zajedno sa parazitnim nematodama-cistolikim nematodama krompira (CNK): Globodera rostochiensis
(Wollenweber) Behrens i G. pallida (Stone) Behrens, dve nematodne vrste koje imaju karantinski status.
Morfologija cistolikih nematoda krompira je do skoro bila jedini način identifikacije ovih karantinskih organizama. U
poslednje dve decenije, molekularne analize kao novi trendovi u modernoj poljoprivredi, su doprineli bržoj i efikasnijoj
identifikaciji ovih vrsta, omogućavajaći uvid u genetičku strukturu onih delova koji su praktično bili nedostupni
morfološkim studijama. Nematode su prisutne u svim evropskim regionima gajenja krompira, posebno na Balkanskom
(Humskom) poluostrvu, pojedinačno ili obe zajedno.
Prikupljeni uzorci cista su pronađeni u zemlji poreklom iz useva semenskog krompira u selu pored Gornjeg Milanovca,
posle zvanične fitosanitarne kontrole. Pojedinačne ciste su korišćene za ekstrakciju DNK sa Dneasy blood & tissue kitom.
PCR je urađen sa prajmerima za direktno sekvenciranje: TW81 and AB28. ITS1-5.8S-ITS2 regioni CNK su korišćeni za
potvrdu identiteta vrste, zajedno sa morfološkom karakterizacijom. Prema EPPO Standardu, morfološka identifikacija
obuhvata karakteristike larvi i cisti, zapravo dužinu i oblik stileta larvi, prečnik vulvalnog bazena cisti, distancu između
vulve i anusa, Granekov odnos i broj kutikularnih nabora u perianalnoj oblasti.
Rezultati su potvrdili identitet vrste. Morfologija naše populacije G. rostochiensis je bila slična prethodno opisanim
domaćim i stranim populacijama. Stepen sličnosti je predstavljen i kao procenat direktnog sparivanja, tj. parne distance.
Filogenetske analize su ukazale na mogućeg pretka naše populacije CNK, predstavljajući evolutivne odnose svetskih
populacija G. rostochiensis i filogenetsko mesto srpske populacije.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi
C3  - 8. international conference sustainable postharvest and food technologies - INOPTEP 2023 i 35. scientific - professional conference processing and energy in agriculture - PTEP 2023 - Book of abstracts
T1  - Molecular and morphological detection of globodera rostochiensis (nematoda: Heteroderidae) in a seed potato crop
SP  - 97
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rik_1167
ER  - 
@conference{
author = "Oro, Violeta and Stanisavljević, Rade and Tabaković, Marijenka and Djokić, Dragoslav",
year = "2023",
abstract = "Potato (Solanum tuberosum L.) is one of four major food crops in the world beside wheat, maize and rice. The plant
originated in the highlands of Peru particularly the region around Lake Titicaca and it was first domesticated at least 7
000 years ago. The food security provided by potato and maize allowed the development and survival of civilizations
such as, Huari and Inca for centuries. In the 16th century the Spanish conquistadores searching for the "treasure of the
Andes" brought to Europe, beside gold, potato along with its parasites – the potato cyst nematodes (PCN): Globodera
rostochiensis (Wollenweber) Behrens and G. pallida (Stone) Behrens, two nematode species that have quarantine status.
The morphology of potato cyst nematodes was until recently almost the only way to identify these quarantine organisms.
In the last two decades, molecular analyses as new trends in modern agriculture, contributed to faster and more efficient
identification of these species and allowed insight into the genetic structure of those parts that were practically inaccessible
by morphological studies. The nematodes are present in all European potato growing regions, especially in the Balkan
(Helm) peninsula, either PCN or both are reported.
The collected specimens of cysts were found in soil originating from a seed potato crop in a village near Gornji Milanovac
after the official phytosanitary control in 2022. Individual cysts were used for DNA extraction with a Dneasy blood &
tissue kit. The PCR was done with primers for direct sequencing: TW81 and AB28. The ITS1-5.8S-ITS2 region of PCN
is used for confirmation of species identity together with its morphological characterization. According to EPPO
Standards, the morphological identification comprised larval and cyst characteristics, namely larval stylet length and stylet
knob shape, cyst vulval basin diameter, distance between vulva and anus, Granek´s ratio, and number of cuticular ridges
in perineal area.
Results confirmed the species identity. The morphology of our population of G. rostochiensis was similar to the previously
reported domestic and foreign populations. The degree of similarity was expressed as a percentage of direct matching i.e.
pairwise distances. Phylogenetic analyses indicated a possible ancestor of our PCN population showing evolutionary
relationships among world populations of G. rostochiensis and a phylogenetic placement of the Serbian population., Krompir (Solanum tuberosum L.) je jedan od četiri glavna prehrambena useva u svetu pored pšenice, kukuruza i pirinča.
Biljka potiče iz planinskog pojasa Perua, posebno iz regiona oko jezera Titikaka i prvi put je odomaćena pre najmanje 7
000 godina. Sigurnost u hrani koja je nastala gajenjem krompira i kukuruza je omogućila vekovni razvoj i opstanak
civilizacija kao što su Huari i Inke. U 16. veku su španski osvajači tražeći "blago sa Anda" pored zlata, u Evropu doneli i
krompir zajedno sa parazitnim nematodama-cistolikim nematodama krompira (CNK): Globodera rostochiensis
(Wollenweber) Behrens i G. pallida (Stone) Behrens, dve nematodne vrste koje imaju karantinski status.
Morfologija cistolikih nematoda krompira je do skoro bila jedini način identifikacije ovih karantinskih organizama. U
poslednje dve decenije, molekularne analize kao novi trendovi u modernoj poljoprivredi, su doprineli bržoj i efikasnijoj
identifikaciji ovih vrsta, omogućavajaći uvid u genetičku strukturu onih delova koji su praktično bili nedostupni
morfološkim studijama. Nematode su prisutne u svim evropskim regionima gajenja krompira, posebno na Balkanskom
(Humskom) poluostrvu, pojedinačno ili obe zajedno.
Prikupljeni uzorci cista su pronađeni u zemlji poreklom iz useva semenskog krompira u selu pored Gornjeg Milanovca,
posle zvanične fitosanitarne kontrole. Pojedinačne ciste su korišćene za ekstrakciju DNK sa Dneasy blood & tissue kitom.
PCR je urađen sa prajmerima za direktno sekvenciranje: TW81 and AB28. ITS1-5.8S-ITS2 regioni CNK su korišćeni za
potvrdu identiteta vrste, zajedno sa morfološkom karakterizacijom. Prema EPPO Standardu, morfološka identifikacija
obuhvata karakteristike larvi i cisti, zapravo dužinu i oblik stileta larvi, prečnik vulvalnog bazena cisti, distancu između
vulve i anusa, Granekov odnos i broj kutikularnih nabora u perianalnoj oblasti.
Rezultati su potvrdili identitet vrste. Morfologija naše populacije G. rostochiensis je bila slična prethodno opisanim
domaćim i stranim populacijama. Stepen sličnosti je predstavljen i kao procenat direktnog sparivanja, tj. parne distance.
Filogenetske analize su ukazale na mogućeg pretka naše populacije CNK, predstavljajući evolutivne odnose svetskih
populacija G. rostochiensis i filogenetsko mesto srpske populacije.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi",
journal = "8. international conference sustainable postharvest and food technologies - INOPTEP 2023 i 35. scientific - professional conference processing and energy in agriculture - PTEP 2023 - Book of abstracts",
title = "Molecular and morphological detection of globodera rostochiensis (nematoda: Heteroderidae) in a seed potato crop",
pages = "97-98",
url = "https://hdl.handle.net/21.15107/rcub_rik_1167"
}
Oro, V., Stanisavljević, R., Tabaković, M.,& Djokić, D.. (2023). Molecular and morphological detection of globodera rostochiensis (nematoda: Heteroderidae) in a seed potato crop. in 8. international conference sustainable postharvest and food technologies - INOPTEP 2023 i 35. scientific - professional conference processing and energy in agriculture - PTEP 2023 - Book of abstracts
Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi., 97-98.
https://hdl.handle.net/21.15107/rcub_rik_1167
Oro V, Stanisavljević R, Tabaković M, Djokić D. Molecular and morphological detection of globodera rostochiensis (nematoda: Heteroderidae) in a seed potato crop. in 8. international conference sustainable postharvest and food technologies - INOPTEP 2023 i 35. scientific - professional conference processing and energy in agriculture - PTEP 2023 - Book of abstracts. 2023;:97-98.
https://hdl.handle.net/21.15107/rcub_rik_1167 .
Oro, Violeta, Stanisavljević, Rade, Tabaković, Marijenka, Djokić, Dragoslav, "Molecular and morphological detection of globodera rostochiensis (nematoda: Heteroderidae) in a seed potato crop" in 8. international conference sustainable postharvest and food technologies - INOPTEP 2023 i 35. scientific - professional conference processing and energy in agriculture - PTEP 2023 - Book of abstracts (2023):97-98,
https://hdl.handle.net/21.15107/rcub_rik_1167 .

Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed properties

Tabaković, Marijenka; Dragicevic, Vesna; Brankov, Milan; Stanisavljević, Rade; Poštić, Dobrivoj; Peric, Vesna; Oro, Violeta

(Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi, 2023)

TY  - JOUR
AU  - Tabaković, Marijenka
AU  - Dragicevic, Vesna
AU  - Brankov, Milan
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Peric, Vesna
AU  - Oro, Violeta
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1165
AB  - Essential oils (EOs) are widely studied in agriculture. The study's objective was to examine the impact of EOs on alfalfa
(Medicago sativa L.) seed dormancy. The four different varieties of alfalfa were used for the experiment (Zaječarka-83, Banatska-VS,
K-28, Novosadska H-11). Two essential oils, lavender (Lavandula angustifolia Mill.) and peppermint (Mentha piperita L.) were
applied at four concentrations:1%, 0.5%, 0.2%, and 0.02%, along with water as a control. Germination, dormant seeds and dead
seeds were evaluated in a laboratory setting according to ISTA rules. The type of EOs had no discernible influence on germinated
seeds, dormancy, and dead seeds. Both EOs in concentrations of 1% and 0.5% inhibited seed germination. The maximum
germination of 91.66% was achieved with the Novosadska H-11 variety using lavender oil at a concentration of 0.02%, with reduced
dormancy. Varieties Zaječarka-83 and Banatska-VS had the highest level of dead and dormant seeds when lavender and peppermint
EOs were applied at a concentration of 0.2%. This study showed that both EOs at a concentration of 0.02% had a stimulatory effect
on seed germination, simultaneously reducing seed dormancy, emphasizing their potential use for seed quality improvement in
organic farming
AB  - Održiv sistem uključuje korišćenje prirodnih resursa za zaštitu bilja, suzbijanje bolesti, štetočina i korova bez upotrebe sintetičkih
hemikalija. Etarska ulja (EO) su dobro poznati metaboliti koji imaju potencijalnu primenu u poljoprivredi. Cilj studije je bio da se
utvrdi značaj delovanja Eunamirovanje (dormantnost) semenalucerke (Medicago sativa L.).Za ogled je korišćen semenski material
četiri sorte lucerke (Zaječarka-83, Banatska-VS, K-28, Novosadska H-11). Dva EO, lavande (Lavandula angustifolia Mill.) i pitome
nane (Mentha piperita L.) primenjena su na semenu lucerke u četiri koncentracije: 1%, 0,5%, 0,2% i 0,02%, zajedno sa vodom
kaokontrolom. Ulja pitome nane i lavande korišćena u eksperimentu su komercijalna ulja. Klijavost, dormantnost i mrtvo seme su
procenjeni u laboratorijskim uslovima prema ISTA pravilima, u petrijevim posudama na filter papiru. Klijanje je testiran ou komori
za klijanje. Rezultati su pokazali da vrsta EO nije imala značajan uticaj na klijanje semena, mirovanje i mrtvo seme. Koncentracija
ulja je bila najznačajniji faktor koji je uticao na fiziološke karakteristike. Oba ulja u koncentracijama od 1% i 0,5% su inhibirala
klijanje. Maksimalna klijavost od 91,66% je postignuta kod sorte Novosadska H-11 uz korišćenje ulja lavande u koncentraciji 0,02%,
dok se mirovanje smanjilo. Sorte Zaječarka-83 i Banatska-VS su imali najviši nivo mrtvog i dormantnog semena, kada su tretirane
uljem lavande i pitome nane u koncentraciji od 0,2%. Ova studija je pokazala da su oba EO u koncentraciji od 0,02% imala
stimulativni efekat na klijavost semena, i istovremeno smanjila mirovanje semena, naglašavajući njihovu potencijalnu primenu za
poboljšanje kvaliteta semena u organskoj poljoprivredi
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed properties
VL  - 27
IS  - 1
SP  - 8
EP  - 12
DO  - 10.5937/jpea27-43109
ER  - 
@article{
author = "Tabaković, Marijenka and Dragicevic, Vesna and Brankov, Milan and Stanisavljević, Rade and Poštić, Dobrivoj and Peric, Vesna and Oro, Violeta",
year = "2023",
abstract = "Essential oils (EOs) are widely studied in agriculture. The study's objective was to examine the impact of EOs on alfalfa
(Medicago sativa L.) seed dormancy. The four different varieties of alfalfa were used for the experiment (Zaječarka-83, Banatska-VS,
K-28, Novosadska H-11). Two essential oils, lavender (Lavandula angustifolia Mill.) and peppermint (Mentha piperita L.) were
applied at four concentrations:1%, 0.5%, 0.2%, and 0.02%, along with water as a control. Germination, dormant seeds and dead
seeds were evaluated in a laboratory setting according to ISTA rules. The type of EOs had no discernible influence on germinated
seeds, dormancy, and dead seeds. Both EOs in concentrations of 1% and 0.5% inhibited seed germination. The maximum
germination of 91.66% was achieved with the Novosadska H-11 variety using lavender oil at a concentration of 0.02%, with reduced
dormancy. Varieties Zaječarka-83 and Banatska-VS had the highest level of dead and dormant seeds when lavender and peppermint
EOs were applied at a concentration of 0.2%. This study showed that both EOs at a concentration of 0.02% had a stimulatory effect
on seed germination, simultaneously reducing seed dormancy, emphasizing their potential use for seed quality improvement in
organic farming, Održiv sistem uključuje korišćenje prirodnih resursa za zaštitu bilja, suzbijanje bolesti, štetočina i korova bez upotrebe sintetičkih
hemikalija. Etarska ulja (EO) su dobro poznati metaboliti koji imaju potencijalnu primenu u poljoprivredi. Cilj studije je bio da se
utvrdi značaj delovanja Eunamirovanje (dormantnost) semenalucerke (Medicago sativa L.).Za ogled je korišćen semenski material
četiri sorte lucerke (Zaječarka-83, Banatska-VS, K-28, Novosadska H-11). Dva EO, lavande (Lavandula angustifolia Mill.) i pitome
nane (Mentha piperita L.) primenjena su na semenu lucerke u četiri koncentracije: 1%, 0,5%, 0,2% i 0,02%, zajedno sa vodom
kaokontrolom. Ulja pitome nane i lavande korišćena u eksperimentu su komercijalna ulja. Klijavost, dormantnost i mrtvo seme su
procenjeni u laboratorijskim uslovima prema ISTA pravilima, u petrijevim posudama na filter papiru. Klijanje je testiran ou komori
za klijanje. Rezultati su pokazali da vrsta EO nije imala značajan uticaj na klijanje semena, mirovanje i mrtvo seme. Koncentracija
ulja je bila najznačajniji faktor koji je uticao na fiziološke karakteristike. Oba ulja u koncentracijama od 1% i 0,5% su inhibirala
klijanje. Maksimalna klijavost od 91,66% je postignuta kod sorte Novosadska H-11 uz korišćenje ulja lavande u koncentraciji 0,02%,
dok se mirovanje smanjilo. Sorte Zaječarka-83 i Banatska-VS su imali najviši nivo mrtvog i dormantnog semena, kada su tretirane
uljem lavande i pitome nane u koncentraciji od 0,2%. Ova studija je pokazala da su oba EO u koncentraciji od 0,02% imala
stimulativni efekat na klijavost semena, i istovremeno smanjila mirovanje semena, naglašavajući njihovu potencijalnu primenu za
poboljšanje kvaliteta semena u organskoj poljoprivredi",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed properties",
volume = "27",
number = "1",
pages = "8-12",
doi = "10.5937/jpea27-43109"
}
Tabaković, M., Dragicevic, V., Brankov, M., Stanisavljević, R., Poštić, D., Peric, V.,& Oro, V.. (2023). Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed properties. in Journal on Processing and Energy in Agriculture
Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi., 27(1), 8-12.
https://doi.org/10.5937/jpea27-43109
Tabaković M, Dragicevic V, Brankov M, Stanisavljević R, Poštić D, Peric V, Oro V. Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed properties. in Journal on Processing and Energy in Agriculture. 2023;27(1):8-12.
doi:10.5937/jpea27-43109 .
Tabaković, Marijenka, Dragicevic, Vesna, Brankov, Milan, Stanisavljević, Rade, Poštić, Dobrivoj, Peric, Vesna, Oro, Violeta, "Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed properties" in Journal on Processing and Energy in Agriculture, 27, no. 1 (2023):8-12,
https://doi.org/10.5937/jpea27-43109 . .
1

Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality

Tabaković, Marijenka; Brankov, Milan; Dragičević, Vesna; Stanisavljević, Rade; Poštić, Dobrivoj; Rakić, Sveto; Oro, Violeta

(Novi Sad : National Society of Processing and Energy in Agriculture, 2023)

TY  - GEN
AU  - Tabaković, Marijenka
AU  - Brankov, Milan
AU  - Dragičević, Vesna
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Rakić, Sveto
AU  - Oro, Violeta
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1157
AB  - Natural allelopathic substances are products or metabolites of some plants, released into the environment, and they have
the potential to be used in sustainable agricultural management systems. A sustainable system includes the use of natural
resources for plant protection, diseases, pests, and weed control without the use of synthetic chemicals. Essential oils
(Eos) are well-known metabolites utilised in agriculture.
From previous studies, it was known that Eos have toxic effects on phytopathogens as well as an inhibitory effect on seed
germination. Less research has been done on the Eo's ability to reduce dormancy and boost germination. The study's
objective was to ascertain the significance of Eo's impact on alfalfa (Medicago sativa L.) seed dormancy.
The seed material of four different varieties of Medicago sativa was used for the experiment (Zaječarka, Banatska, K-28,
Novosadska). Two essential oils, lavender (Lavandula angustifolia Mill.) and mint (Mentha piperita L.) were applied to
seeds at four concentrations—1%, 0.5%, 0.2%, and 0.02%—along with water as a control. Lavender and mint essential
oils used in the experiment are commercial oils obtained from mint leaves and fresh lavender flowers by distillation.
Germination, dormant seeds and dead seeds were evaluated in a laboratory setting according to ISTA rules, in Petry dishes
on filter paper. A germination cabinet was the equipment for seed germination.
Results showed that the type of Eos had no discernible influence on germinated seeds, dormancy, and dead seeds. The oil
concentration was the most significant factor affecting physiological characteristics. Both oils in concentrations of -1%
and 0.5% inhibited seed germination 100%. The maximum germination at 91,66% was achieved with the Novosadska
variety using lavender oil at a concentration of 0.02%, while dormancy was reduced. Varieties Zaječarka and Banatska
had the highest level of dead and dormant seeds, when lavender and mint Eos were applied at a concentration of 0.2%.
This study showed that both Eos at a concentration of 0.02% had a stimulatory effect on seed germination and
simultaneously reduced seed dormancy, emphasizing their potential use for seed quality improvement in organic farming.
AB  - Prirodne alelopatske supstance su proizvodi ili metaboliti nekih biljaka, koje biljka otpušta u životnu sredinu, i imaju
potencijal da se koriste u upravljanju sistema održive poljoprivrede. Održiv sistem uključuje korišćenje prirodnih resursa
za zaštitu bilja, suzbijanje bolesti, štetočina i korova bez upotrebe sintetičkih hemikalija. Esencijalna ulja (Eu) su dobro
poznati metaboliti koji se koriste u poljoprivredi.
Iz ranijih istraživanja poznato je da Eu imaju toksično dejstvo na fitopatogene, kao i inhibitorno dejstvo na klijanje
semena. Manje istraživanja je rađeno o sposobnosti Eu da smanje mirovanje i povećaju klijavost semena. Cilj studije je
bio da se utvrdi značaj delovanja Eu na mirovanje (dormantnost) semena lucerke (Medicago sativa L.).
Za ogled korišćen je semenski materijal četiri sorte lucerke (Zaječarka, Banatska, K-28, Novosadska). Dva Eu, lavanda
(Lavandula angustifolia Mill.) i nana (Mentha piperita L.) primenjena su na semenu lucerke u četiri koncentracije — 1%,
0,5%, 0,2% i 0,02% — zajedno sa vodom kao kontrolom. Ulja nane i lavande korišćena u eksperimentu su komercijalna
ulja dobijena destilacijom listova nane i svežih cvetova lavande. Klijavost, dormantnost i mrtvo seme su procenjeni u
laboratorijskim uslovima prema ISTA pravilima, u petrijevim posudama na filter papiru. Klijanje je rađeno u komori za
klijanje.
Rezultati su pokazali da vrsta Eu nije imala vidljiv uticaj na klijanje semena, mirovanje i mrtvo seme. Koncentracija ulja
je bila najznačajniji faktor koji je uticao na fiziološke karakteristike. Oba ulja u koncentracijama od 1% i 0,5% su
inhibirala klijanje 100%. Maksimalna klijavost 91,66% je postignuta kod sorte Novosadska uz korišćenje ulja lavande u
koncentraciji 0,02%, dok se mirovanje smanjilo. Sorte Zaječarka i Banatska su imali najviši nivo mrtvog i dormantnog
semena, kada su tretirane uljem lavande i nane u koncentraciji od 0,2%.
Ova studija je pokazala da su oba Eu u koncentraciji od 0,02% imala stimulativni efekat na klijavost semena, i istovremeno
smanjila mirovanje semena, naglašavajući njihovu potencijalnu primenu za poboljšanje kvaliteta semena u organskoj
poljoprivredi.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
T2  - I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur
T1  - Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality
T1  - Primena esencijalnih ulja lavande i nane za poboljšanje kvaliteta semena lucerke (Medicago sativa L.)
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_rik_1157
ER  - 
@misc{
author = "Tabaković, Marijenka and Brankov, Milan and Dragičević, Vesna and Stanisavljević, Rade and Poštić, Dobrivoj and Rakić, Sveto and Oro, Violeta",
year = "2023",
abstract = "Natural allelopathic substances are products or metabolites of some plants, released into the environment, and they have
the potential to be used in sustainable agricultural management systems. A sustainable system includes the use of natural
resources for plant protection, diseases, pests, and weed control without the use of synthetic chemicals. Essential oils
(Eos) are well-known metabolites utilised in agriculture.
From previous studies, it was known that Eos have toxic effects on phytopathogens as well as an inhibitory effect on seed
germination. Less research has been done on the Eo's ability to reduce dormancy and boost germination. The study's
objective was to ascertain the significance of Eo's impact on alfalfa (Medicago sativa L.) seed dormancy.
The seed material of four different varieties of Medicago sativa was used for the experiment (Zaječarka, Banatska, K-28,
Novosadska). Two essential oils, lavender (Lavandula angustifolia Mill.) and mint (Mentha piperita L.) were applied to
seeds at four concentrations—1%, 0.5%, 0.2%, and 0.02%—along with water as a control. Lavender and mint essential
oils used in the experiment are commercial oils obtained from mint leaves and fresh lavender flowers by distillation.
Germination, dormant seeds and dead seeds were evaluated in a laboratory setting according to ISTA rules, in Petry dishes
on filter paper. A germination cabinet was the equipment for seed germination.
Results showed that the type of Eos had no discernible influence on germinated seeds, dormancy, and dead seeds. The oil
concentration was the most significant factor affecting physiological characteristics. Both oils in concentrations of -1%
and 0.5% inhibited seed germination 100%. The maximum germination at 91,66% was achieved with the Novosadska
variety using lavender oil at a concentration of 0.02%, while dormancy was reduced. Varieties Zaječarka and Banatska
had the highest level of dead and dormant seeds, when lavender and mint Eos were applied at a concentration of 0.2%.
This study showed that both Eos at a concentration of 0.02% had a stimulatory effect on seed germination and
simultaneously reduced seed dormancy, emphasizing their potential use for seed quality improvement in organic farming., Prirodne alelopatske supstance su proizvodi ili metaboliti nekih biljaka, koje biljka otpušta u životnu sredinu, i imaju
potencijal da se koriste u upravljanju sistema održive poljoprivrede. Održiv sistem uključuje korišćenje prirodnih resursa
za zaštitu bilja, suzbijanje bolesti, štetočina i korova bez upotrebe sintetičkih hemikalija. Esencijalna ulja (Eu) su dobro
poznati metaboliti koji se koriste u poljoprivredi.
Iz ranijih istraživanja poznato je da Eu imaju toksično dejstvo na fitopatogene, kao i inhibitorno dejstvo na klijanje
semena. Manje istraživanja je rađeno o sposobnosti Eu da smanje mirovanje i povećaju klijavost semena. Cilj studije je
bio da se utvrdi značaj delovanja Eu na mirovanje (dormantnost) semena lucerke (Medicago sativa L.).
Za ogled korišćen je semenski materijal četiri sorte lucerke (Zaječarka, Banatska, K-28, Novosadska). Dva Eu, lavanda
(Lavandula angustifolia Mill.) i nana (Mentha piperita L.) primenjena su na semenu lucerke u četiri koncentracije — 1%,
0,5%, 0,2% i 0,02% — zajedno sa vodom kao kontrolom. Ulja nane i lavande korišćena u eksperimentu su komercijalna
ulja dobijena destilacijom listova nane i svežih cvetova lavande. Klijavost, dormantnost i mrtvo seme su procenjeni u
laboratorijskim uslovima prema ISTA pravilima, u petrijevim posudama na filter papiru. Klijanje je rađeno u komori za
klijanje.
Rezultati su pokazali da vrsta Eu nije imala vidljiv uticaj na klijanje semena, mirovanje i mrtvo seme. Koncentracija ulja
je bila najznačajniji faktor koji je uticao na fiziološke karakteristike. Oba ulja u koncentracijama od 1% i 0,5% su
inhibirala klijanje 100%. Maksimalna klijavost 91,66% je postignuta kod sorte Novosadska uz korišćenje ulja lavande u
koncentraciji 0,02%, dok se mirovanje smanjilo. Sorte Zaječarka i Banatska su imali najviši nivo mrtvog i dormantnog
semena, kada su tretirane uljem lavande i nane u koncentraciji od 0,2%.
Ova studija je pokazala da su oba Eu u koncentraciji od 0,02% imala stimulativni efekat na klijavost semena, i istovremeno
smanjila mirovanje semena, naglašavajući njihovu potencijalnu primenu za poboljšanje kvaliteta semena u organskoj
poljoprivredi.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture",
journal = "I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur",
title = "Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality, Primena esencijalnih ulja lavande i nane za poboljšanje kvaliteta semena lucerke (Medicago sativa L.)",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_rik_1157"
}
Tabaković, M., Brankov, M., Dragičević, V., Stanisavljević, R., Poštić, D., Rakić, S.,& Oro, V.. (2023). Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality. in I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur
Novi Sad : National Society of Processing and Energy in Agriculture., 139.
https://hdl.handle.net/21.15107/rcub_rik_1157
Tabaković M, Brankov M, Dragičević V, Stanisavljević R, Poštić D, Rakić S, Oro V. Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality. in I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur. 2023;:139.
https://hdl.handle.net/21.15107/rcub_rik_1157 .
Tabaković, Marijenka, Brankov, Milan, Dragičević, Vesna, Stanisavljević, Rade, Poštić, Dobrivoj, Rakić, Sveto, Oro, Violeta, "Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality" in I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur (2023):139,
https://hdl.handle.net/21.15107/rcub_rik_1157 .

Procena stabilnosti sadržaja ulja u zrnu ranostasnih genotipova soje

Peric, Vesna; Srebric, Mirjana; Nikolić, Valentina; Tabaković, Marijenka

(Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi, 2022)

TY  - CONF
AU  - Peric, Vesna
AU  - Srebric, Mirjana
AU  - Nikolić, Valentina
AU  - Tabaković, Marijenka
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1168
AB  - in the grain. The processing industry, as well as individual agricultural producers, pays special attention
to these two parameters, setting requirements for varieties with high oil content for industrial
processing, or varieties with high protein content for animal feed. Therefore, breeding practice is aimed
at developing varieties not only of high yield but also varieties of improved grain quality. Soybean
genotypes maintained in the collection of the Maize Research Institute Zemun Polje are characterized
by a protein content ranging from 36.3% to 43.2%, and an oil content ranging from 15.6% to 22.0%.
Both traits are of a complex quantitative nature, determined both by genetic factors and the influence of
environmental conditions during their accumulation in the grain, as well as the interaction of genotype
and environment.
The aim of this study was to examine the value of genotype × environment interaction for oil content in
soybean genotypes from the collection of Maize Research Institute Zemun Polje and to identify stable
sources that can be utilized as starting material for breeding for oil content.
The experiment included 14 genotypes of soybean from maturity group 00 (very early varieties). The
field trials were set up over two years, at two locations (Zemun Polje and Pancevo), according to a
completely randomized block design with three replications. After harvest, the oil content in the grain
was measured on a NIRT device "Infraneo", Chopin Technologies. The linear-bilinear AMMI-1 model
was applied to analyze the genotype × environment interaction for grain oil content.
A large part of the variation (80.91%) of the genotype × environment interaction for the grain oil
content in soybean genotypes was explained by the first interaction axis of the AMMI-1 model. The
differences in the main effects of the examined environments were not large, since all environments
had a value of oil content close to the general average.
Four environments had a variable influence on the examined genotypes. For both locations, a positive
interaction effect was found in 2012 and a negative interaction effect in 2011, with the genotypes tested
in Zemun Polje in 2012 being the most unstable, while the genotypes tested in 2011 showed
approximately equal stability at both locations.
A number of genotypes (Canatto, Kabott, Olima, Gi 291 / 70-79, Krajina, Agassiz, Maple Presto and
Luso) were distributed close to the stability line. Genotypes with above the average oil content and
high stability (Maple Presto and Ljuso) deserved special attention, as well as the Agassiz genotype,
which had the maximum average value of this parameter, and a small value of interaction with
environments. Genotypes of low average values of oil content (Mini Soybeans and Progress) had very
poor stability, which can be attributed to their divergent germplasm and specific response to
environmental conditions that differ from the conditions in the region of their origin.
AB  - Prerađivačka industrija kao i individualni poljoprivredni proizvođači obraćaju posebnu pažnju na ova
dva parametra, postavljajući zahteve za sortama visokog sadržaja ulja za industrijsku preradu, ili
sortama visokog sadržaja proteina za dobijanje stočne hrane. Stoga je selekcionerski rad usmeren je ka
stvaranju sorti ne samo visokog prinosa već i sorti poboljšanog kvaliteta zrna. Genotipovi soje koji se
čuvaju u kolekciji Instituta za kukuruz Zemun Polje odlikuju se sadržajem proteina koji varira od
36.3% do 43.2%, i sadržajem ulja u rasponu od 15.6% do22.0%. Oba svojstva su kompleksne
kvantitativne prirode, determinisana kako naslednim faktorima tako i uticajem uslova spoljašnje
sredine u vreme njihove akumulacije u zrnu, kao i interakcijom genotipa i spoljašnje sredine.
Cilj ovog rada bio je da se ispita vrednost interakcije genotip × spoljašnja sredina za sadržaj ulja kod
genotipova soje iz kolekcije Instituta za kukuruz Zemun Polje i otkriju stabilni izvori koji mogu
poslužiti kao početni materijal za oplemenjivanje na sadržaj ulja.
Eksperimentom je obuhvaćeno 14 genotipova soje grupe zrenja 00 (veoma rane sorte). Ogledi su
postavljeni u toku dve godine, na dve lokacije (Zemun Polje i Pančevo), po potpuno slučajnom blok
dizajnu u tri ponavljanja. Nakon žetve, sadržaj ulja u semenu izmeren je na uređaju NIRT tehnologije
„Infraneo“, Chopin Technologies. Za analizu interakcije genotipa i spoljašnje sredine za sadržaj ulja u
zrnu primenjen je linearno-bilinearni AMMI-1 model.
Veliki deo varijacije (80,91%) interakcije genotipa i spoljašnje sredine za sadržaj ulja u zrnu ispitivanih
genotipova soje objašnjen je prvom interakcijskom osom AMMI-1 modela. Razlike u glavnim efektima
ispitivanih spoljašnjih sredina nisu bile velike, s obzirom da su sve sredine imale vrednost sadržaja ulja
blizu opšteg proseka.
Četiri spoljašnje sredine imale su varijabilan uticaj na ispitivane genotipove. Za oba lokaliteta utvrđen
je pozitivan interakcijski efekat u 2012. i negativan interakcijski efekat u 2011. godini, pri čemu su
genotipovi ispitivani u Zemun Polju 2012. godine bili najnestabilniji, dok su genotipovi ispitivani u
toku 2011. godine pokazali približno jednaku stabilnost na obe lokacije
Veći broj genotipova (Canatto, Kabott, Olima, Gi 291/70-79, Krajina, Agassiz, Maple Presto i Ljuso)
bio je raspoređen blizu linije stabilnosti, pri čemu posebnu pažnju zaslužuju genotipovi iznad posečnog
sadržaja ulja i visoke stabilnosti (Maple Presto i Ljuso) kao i genotip Agassiz, koji je imao maksimalnu
prosečnu vrednost ovog parametra, i malu vrednost interakcije sa spoljašnjim sredinama. Genotipovi
niskih prosečnih vrednosti sadržaja ulja (Mini Soja i Progres) imali su veoma slabu stabilnost, što se
može pripisati njihovoj divergentnoj germplazmi i specifičnoj reakciji na uslove ispitivanja koji se
razlikuju u odnosu na uslove regiona u kojem su ove sorte selekcionisane.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi
C3  - 34. nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi – PTEP 2022
T1  - Procena stabilnosti sadržaja ulja u zrnu ranostasnih genotipova soje
T1  - Assessment of grain oil content stability in early maturing soybean genotypes
SP  - 70
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_rik_1168
ER  - 
@conference{
author = "Peric, Vesna and Srebric, Mirjana and Nikolić, Valentina and Tabaković, Marijenka",
year = "2022",
abstract = "in the grain. The processing industry, as well as individual agricultural producers, pays special attention
to these two parameters, setting requirements for varieties with high oil content for industrial
processing, or varieties with high protein content for animal feed. Therefore, breeding practice is aimed
at developing varieties not only of high yield but also varieties of improved grain quality. Soybean
genotypes maintained in the collection of the Maize Research Institute Zemun Polje are characterized
by a protein content ranging from 36.3% to 43.2%, and an oil content ranging from 15.6% to 22.0%.
Both traits are of a complex quantitative nature, determined both by genetic factors and the influence of
environmental conditions during their accumulation in the grain, as well as the interaction of genotype
and environment.
The aim of this study was to examine the value of genotype × environment interaction for oil content in
soybean genotypes from the collection of Maize Research Institute Zemun Polje and to identify stable
sources that can be utilized as starting material for breeding for oil content.
The experiment included 14 genotypes of soybean from maturity group 00 (very early varieties). The
field trials were set up over two years, at two locations (Zemun Polje and Pancevo), according to a
completely randomized block design with three replications. After harvest, the oil content in the grain
was measured on a NIRT device "Infraneo", Chopin Technologies. The linear-bilinear AMMI-1 model
was applied to analyze the genotype × environment interaction for grain oil content.
A large part of the variation (80.91%) of the genotype × environment interaction for the grain oil
content in soybean genotypes was explained by the first interaction axis of the AMMI-1 model. The
differences in the main effects of the examined environments were not large, since all environments
had a value of oil content close to the general average.
Four environments had a variable influence on the examined genotypes. For both locations, a positive
interaction effect was found in 2012 and a negative interaction effect in 2011, with the genotypes tested
in Zemun Polje in 2012 being the most unstable, while the genotypes tested in 2011 showed
approximately equal stability at both locations.
A number of genotypes (Canatto, Kabott, Olima, Gi 291 / 70-79, Krajina, Agassiz, Maple Presto and
Luso) were distributed close to the stability line. Genotypes with above the average oil content and
high stability (Maple Presto and Ljuso) deserved special attention, as well as the Agassiz genotype,
which had the maximum average value of this parameter, and a small value of interaction with
environments. Genotypes of low average values of oil content (Mini Soybeans and Progress) had very
poor stability, which can be attributed to their divergent germplasm and specific response to
environmental conditions that differ from the conditions in the region of their origin., Prerađivačka industrija kao i individualni poljoprivredni proizvođači obraćaju posebnu pažnju na ova
dva parametra, postavljajući zahteve za sortama visokog sadržaja ulja za industrijsku preradu, ili
sortama visokog sadržaja proteina za dobijanje stočne hrane. Stoga je selekcionerski rad usmeren je ka
stvaranju sorti ne samo visokog prinosa već i sorti poboljšanog kvaliteta zrna. Genotipovi soje koji se
čuvaju u kolekciji Instituta za kukuruz Zemun Polje odlikuju se sadržajem proteina koji varira od
36.3% do 43.2%, i sadržajem ulja u rasponu od 15.6% do22.0%. Oba svojstva su kompleksne
kvantitativne prirode, determinisana kako naslednim faktorima tako i uticajem uslova spoljašnje
sredine u vreme njihove akumulacije u zrnu, kao i interakcijom genotipa i spoljašnje sredine.
Cilj ovog rada bio je da se ispita vrednost interakcije genotip × spoljašnja sredina za sadržaj ulja kod
genotipova soje iz kolekcije Instituta za kukuruz Zemun Polje i otkriju stabilni izvori koji mogu
poslužiti kao početni materijal za oplemenjivanje na sadržaj ulja.
Eksperimentom je obuhvaćeno 14 genotipova soje grupe zrenja 00 (veoma rane sorte). Ogledi su
postavljeni u toku dve godine, na dve lokacije (Zemun Polje i Pančevo), po potpuno slučajnom blok
dizajnu u tri ponavljanja. Nakon žetve, sadržaj ulja u semenu izmeren je na uređaju NIRT tehnologije
„Infraneo“, Chopin Technologies. Za analizu interakcije genotipa i spoljašnje sredine za sadržaj ulja u
zrnu primenjen je linearno-bilinearni AMMI-1 model.
Veliki deo varijacije (80,91%) interakcije genotipa i spoljašnje sredine za sadržaj ulja u zrnu ispitivanih
genotipova soje objašnjen je prvom interakcijskom osom AMMI-1 modela. Razlike u glavnim efektima
ispitivanih spoljašnjih sredina nisu bile velike, s obzirom da su sve sredine imale vrednost sadržaja ulja
blizu opšteg proseka.
Četiri spoljašnje sredine imale su varijabilan uticaj na ispitivane genotipove. Za oba lokaliteta utvrđen
je pozitivan interakcijski efekat u 2012. i negativan interakcijski efekat u 2011. godini, pri čemu su
genotipovi ispitivani u Zemun Polju 2012. godine bili najnestabilniji, dok su genotipovi ispitivani u
toku 2011. godine pokazali približno jednaku stabilnost na obe lokacije
Veći broj genotipova (Canatto, Kabott, Olima, Gi 291/70-79, Krajina, Agassiz, Maple Presto i Ljuso)
bio je raspoređen blizu linije stabilnosti, pri čemu posebnu pažnju zaslužuju genotipovi iznad posečnog
sadržaja ulja i visoke stabilnosti (Maple Presto i Ljuso) kao i genotip Agassiz, koji je imao maksimalnu
prosečnu vrednost ovog parametra, i malu vrednost interakcije sa spoljašnjim sredinama. Genotipovi
niskih prosečnih vrednosti sadržaja ulja (Mini Soja i Progres) imali su veoma slabu stabilnost, što se
može pripisati njihovoj divergentnoj germplazmi i specifičnoj reakciji na uslove ispitivanja koji se
razlikuju u odnosu na uslove regiona u kojem su ove sorte selekcionisane.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi",
journal = "34. nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi – PTEP 2022",
title = "Procena stabilnosti sadržaja ulja u zrnu ranostasnih genotipova soje, Assessment of grain oil content stability in early maturing soybean genotypes",
pages = "70-71",
url = "https://hdl.handle.net/21.15107/rcub_rik_1168"
}
Peric, V., Srebric, M., Nikolić, V.,& Tabaković, M.. (2022). Procena stabilnosti sadržaja ulja u zrnu ranostasnih genotipova soje. in 34. nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi – PTEP 2022
Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi., 70-71.
https://hdl.handle.net/21.15107/rcub_rik_1168
Peric V, Srebric M, Nikolić V, Tabaković M. Procena stabilnosti sadržaja ulja u zrnu ranostasnih genotipova soje. in 34. nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi – PTEP 2022. 2022;:70-71.
https://hdl.handle.net/21.15107/rcub_rik_1168 .
Peric, Vesna, Srebric, Mirjana, Nikolić, Valentina, Tabaković, Marijenka, "Procena stabilnosti sadržaja ulja u zrnu ranostasnih genotipova soje" in 34. nacionalna konferencija sa međunarodnim učešćem „Procesna tehnika i energetika u poljoprivredi – PTEP 2022 (2022):70-71,
https://hdl.handle.net/21.15107/rcub_rik_1168 .

Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines

Tabaković, Marijenka; Dragicevic, Vesna; Simic, Milena; Stanisavljević, Rade; Poštić, Dobrivoj; Brankov, Milan; Oro, Violeta

(Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi, 2022)

TY  - JOUR
AU  - Tabaković, Marijenka
AU  - Dragicevic, Vesna
AU  - Simic, Milena
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Brankov, Milan
AU  - Oro, Violeta
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1163
AB  - This study aimed to evaluate the influence of environmental conditions and genotype on the yield of three maize lines and the morphological properties of ears and seeds. Three hybrid maize lines were used as material in the study conducted in two consecutive years (2018 and 2019) at one location (Zemun Polje, Belgrade, Serbia). The following parameters were monitored: seed weight (SW), seed volume (V), bulk density (BD), ear length (EL), ear thickness (ET), fraction content and seed yield (MSY). Calibration divided the seed into a small seed fraction of 6.5-8.4 mm (SF) and a large seed fraction of 8.5-11 mm (LF). Seeds smaller than 6.5 mm and larger than 11 mm are presented as seed waste fraction (FW). The average seed yield for all three lines in the first and the second year was 6.13 and 4.66 t ha-1 , respectively. A significant impact of the environment was noticed on seed weights in both years (2018 and 2019), i.e. 327.56 and 251.77 g, respectively. The total variation in yield determined by the morphological characteristics of the seed was R2 =0.514.
AB  - Cilj rada bio je da se oceni uticaj uslova sredine i genotipa na prinos tri linije kukuruza i morfološke osobine klipa. U radu je
korišćeno seme tri linije kukuruza, u dvogodišnjem ogledu izvedenom u 2018 i 2019 godini, na jednoj lokaciji. Parametri koji su
posmatrani bili su prinos zrna (MSY), apsolutna masa semena (SW), zapremina semena (V), gustina semena (BD), dužina klipa (EL),
debljina klipa (ET) i težina klipa (EW). Kalibracijom semena dobijene su tri frakcije semena: sitna, veličine semena 6,5-8,4mm (SF),
krupna, veličine semena 8,5-11mm (KF) i frakcija otpada, veličine semena ispod 6,5mm i iznad 11mm (FW).Prosečan prinos za sve
linije u prvoj godine bio je 6,13 a u drugoj 4,66 t ha-1. Značajan efekat ekoloških uslova bio je i na apsolutnu masu semena (327,56 g
u prvoj; odnosno 251,77 g u drugoj godini). Ovako velika razlika u apsolutnoj masi semena odrazila se i na ukupan odnos sitne (SF)
i krupne frakcije (LF) semena na klipu. Ukupno variranje prinosa klipa determinisano morfološkim osobinama semena bilo je R2
 =
0.514. Sve morfološke osobine klipa i semena međusobno su značajno korelirale na oba nivoa značajnosti (p≤0,01, p≤0,05).
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines
VL  - 26
IS  - 2
SP  - 71
EP  - 74
DO  - 10.5937/jpea26-36609
ER  - 
@article{
author = "Tabaković, Marijenka and Dragicevic, Vesna and Simic, Milena and Stanisavljević, Rade and Poštić, Dobrivoj and Brankov, Milan and Oro, Violeta",
year = "2022",
abstract = "This study aimed to evaluate the influence of environmental conditions and genotype on the yield of three maize lines and the morphological properties of ears and seeds. Three hybrid maize lines were used as material in the study conducted in two consecutive years (2018 and 2019) at one location (Zemun Polje, Belgrade, Serbia). The following parameters were monitored: seed weight (SW), seed volume (V), bulk density (BD), ear length (EL), ear thickness (ET), fraction content and seed yield (MSY). Calibration divided the seed into a small seed fraction of 6.5-8.4 mm (SF) and a large seed fraction of 8.5-11 mm (LF). Seeds smaller than 6.5 mm and larger than 11 mm are presented as seed waste fraction (FW). The average seed yield for all three lines in the first and the second year was 6.13 and 4.66 t ha-1 , respectively. A significant impact of the environment was noticed on seed weights in both years (2018 and 2019), i.e. 327.56 and 251.77 g, respectively. The total variation in yield determined by the morphological characteristics of the seed was R2 =0.514., Cilj rada bio je da se oceni uticaj uslova sredine i genotipa na prinos tri linije kukuruza i morfološke osobine klipa. U radu je
korišćeno seme tri linije kukuruza, u dvogodišnjem ogledu izvedenom u 2018 i 2019 godini, na jednoj lokaciji. Parametri koji su
posmatrani bili su prinos zrna (MSY), apsolutna masa semena (SW), zapremina semena (V), gustina semena (BD), dužina klipa (EL),
debljina klipa (ET) i težina klipa (EW). Kalibracijom semena dobijene su tri frakcije semena: sitna, veličine semena 6,5-8,4mm (SF),
krupna, veličine semena 8,5-11mm (KF) i frakcija otpada, veličine semena ispod 6,5mm i iznad 11mm (FW).Prosečan prinos za sve
linije u prvoj godine bio je 6,13 a u drugoj 4,66 t ha-1. Značajan efekat ekoloških uslova bio je i na apsolutnu masu semena (327,56 g
u prvoj; odnosno 251,77 g u drugoj godini). Ovako velika razlika u apsolutnoj masi semena odrazila se i na ukupan odnos sitne (SF)
i krupne frakcije (LF) semena na klipu. Ukupno variranje prinosa klipa determinisano morfološkim osobinama semena bilo je R2
 =
0.514. Sve morfološke osobine klipa i semena međusobno su značajno korelirale na oba nivoa značajnosti (p≤0,01, p≤0,05).",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines",
volume = "26",
number = "2",
pages = "71-74",
doi = "10.5937/jpea26-36609"
}
Tabaković, M., Dragicevic, V., Simic, M., Stanisavljević, R., Poštić, D., Brankov, M.,& Oro, V.. (2022). Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines. in Journal on Processing and Energy in Agriculture
Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi., 26(2), 71-74.
https://doi.org/10.5937/jpea26-36609
Tabaković M, Dragicevic V, Simic M, Stanisavljević R, Poštić D, Brankov M, Oro V. Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines. in Journal on Processing and Energy in Agriculture. 2022;26(2):71-74.
doi:10.5937/jpea26-36609 .
Tabaković, Marijenka, Dragicevic, Vesna, Simic, Milena, Stanisavljević, Rade, Poštić, Dobrivoj, Brankov, Milan, Oro, Violeta, "Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines" in Journal on Processing and Energy in Agriculture, 26, no. 2 (2022):71-74,
https://doi.org/10.5937/jpea26-36609 . .
1

Conjunctive effect of environment and genotype in maize seed production.

Tabaković, Marijenka; Peric, Vesna; Dragicevic, Vesna; Simic, Milena; Stanisavljević, Rade; Poštić, Dobrivoj; Oro, Violeta

(Banja Luka : Narodna i univerzitetska biblioteka Republike Srpske, 2022)

TY  - CONF
AU  - Tabaković, Marijenka
AU  - Peric, Vesna
AU  - Dragicevic, Vesna
AU  - Simic, Milena
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Oro, Violeta
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1158
AB  - Significant sources of normal plant development are the amount of available water, light,
temperature and nutrients. This study aimed to examine to what extent the relationship
between plant genetic structure and environmental conditions affects habitus and plant yield.
In the two-year research, 2019 (Y1), and 2020 (Y2), with three maize lines (L1, L2, L3)
produced at the Maize Research Institute, experiments were performed to assess the impact of
genotype and environmental conditions on plant height to tassel (PHT), plant height to ear
(PHE), ear weight (EW), cob weight (CW) and grain yield (GY). Seeds of different sizes
were used in three sowings: large (S1), small (S2), and undivided (S3). The results of the
PHE trial in the first year indicated a dominant genotype effect. L1 for all three sowings by
seed size had the lowest cob position, S3G1 (69 cm), while L3 had the highest, (86.72 cm)
for S3G1. In the second year of the study, environmental conditions and seed size
significantly affected (p≤0.05) the PHE, as well as the PHT. The significance of the year
effect was not confirmed by the weight of the cob. The highest yield was achieved for
L1Y1S1 at 9.01 t ha-1 and the lowest for L1Y2S3 at 2.18 t ha-1. Significant mutual effects of
factors on the variability of traits are Y × L, Y × S, and Y × L × S. Differences in
environmental conditions significantly affect the variability of maize corn seed properties.
These effects can be reduced by proper genotype selection and the sowing of uniform size
seeds.
PB  - Banja Luka : Narodna i univerzitetska biblioteka Republike Srpske
C3  - 13. International scientific agriculture symposium “AGROSYM 2022 - Book of proceedings
T1  - Conjunctive effect of environment and genotype in maize seed production.
UR  - https://hdl.handle.net/21.15107/rcub_rik_1158
ER  - 
@conference{
author = "Tabaković, Marijenka and Peric, Vesna and Dragicevic, Vesna and Simic, Milena and Stanisavljević, Rade and Poštić, Dobrivoj and Oro, Violeta",
year = "2022",
abstract = "Significant sources of normal plant development are the amount of available water, light,
temperature and nutrients. This study aimed to examine to what extent the relationship
between plant genetic structure and environmental conditions affects habitus and plant yield.
In the two-year research, 2019 (Y1), and 2020 (Y2), with three maize lines (L1, L2, L3)
produced at the Maize Research Institute, experiments were performed to assess the impact of
genotype and environmental conditions on plant height to tassel (PHT), plant height to ear
(PHE), ear weight (EW), cob weight (CW) and grain yield (GY). Seeds of different sizes
were used in three sowings: large (S1), small (S2), and undivided (S3). The results of the
PHE trial in the first year indicated a dominant genotype effect. L1 for all three sowings by
seed size had the lowest cob position, S3G1 (69 cm), while L3 had the highest, (86.72 cm)
for S3G1. In the second year of the study, environmental conditions and seed size
significantly affected (p≤0.05) the PHE, as well as the PHT. The significance of the year
effect was not confirmed by the weight of the cob. The highest yield was achieved for
L1Y1S1 at 9.01 t ha-1 and the lowest for L1Y2S3 at 2.18 t ha-1. Significant mutual effects of
factors on the variability of traits are Y × L, Y × S, and Y × L × S. Differences in
environmental conditions significantly affect the variability of maize corn seed properties.
These effects can be reduced by proper genotype selection and the sowing of uniform size
seeds.",
publisher = "Banja Luka : Narodna i univerzitetska biblioteka Republike Srpske",
journal = "13. International scientific agriculture symposium “AGROSYM 2022 - Book of proceedings",
title = "Conjunctive effect of environment and genotype in maize seed production.",
url = "https://hdl.handle.net/21.15107/rcub_rik_1158"
}
Tabaković, M., Peric, V., Dragicevic, V., Simic, M., Stanisavljević, R., Poštić, D.,& Oro, V.. (2022). Conjunctive effect of environment and genotype in maize seed production.. in 13. International scientific agriculture symposium “AGROSYM 2022 - Book of proceedings
Banja Luka : Narodna i univerzitetska biblioteka Republike Srpske..
https://hdl.handle.net/21.15107/rcub_rik_1158
Tabaković M, Peric V, Dragicevic V, Simic M, Stanisavljević R, Poštić D, Oro V. Conjunctive effect of environment and genotype in maize seed production.. in 13. International scientific agriculture symposium “AGROSYM 2022 - Book of proceedings. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1158 .
Tabaković, Marijenka, Peric, Vesna, Dragicevic, Vesna, Simic, Milena, Stanisavljević, Rade, Poštić, Dobrivoj, Oro, Violeta, "Conjunctive effect of environment and genotype in maize seed production." in 13. International scientific agriculture symposium “AGROSYM 2022 - Book of proceedings (2022),
https://hdl.handle.net/21.15107/rcub_rik_1158 .