Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200040 (Maize Research Institute 'Zemun Polje', Belgrade-Zemun)

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Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200040 (Maize Research Institute 'Zemun Polje', Belgrade-Zemun) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200040 (Institut za kukuruz 'Zemun polje', Beograd-Zemun) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200040 (Институт за кукуруз 'Земун поље', Београд-Земун) (sr)
Authors

Publications

Kernel color and fertilization as factors of enhanced maize quality

Dragičević, Vesna; Brankov, Milan; Stoiljkovic, Milovan; Tolimir, Miodrag; Travlos, Ilias; Simić, Milena

(Lausanne : Frontiers, 2022)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Brankov, Milan
AU  - Stoiljkovic, Milovan
AU  - Tolimir, Miodrag
AU  - Travlos, Ilias
AU  - Simić, Milena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1059
AB  - Maize is an important staple crop and a significant source of various nutrients.
We aimed to determine the macronutrients, antioxidants, and essential
elements in maize genotypes (white, yellow, and red kernel) using three
different fertilizers, which could be used as a basis to increase the nutrient
density of maize. The fertilizer treatments used bio- and organic fertilizers as a
sustainable approach, urea, as a commonly used mineral fertilizer, and the
control (no fertilization). We evaluated the yield, concentration of
macronutrient (protein, oil, and starch), nonenzymatic antioxidants
(phenolics, yellow pigment, total glutathione (GSH), and phytic phosphorus),
and reduction capacity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, as
well as essential elements that are commonly deficient in the diet (Mg, Ca, Fe,
Mn, Zn, Cu, and S) and their relationships with phytic acid. The genotype
expressed the strongest effect on the variability of grain yield and the analyzed
grain constituents. The red-kernel hybrid showed the greatest accumulation of
protein, oil, phenolics, and essential elements (Ca, Fe, Cu, and S) than a yellow
and white hybrid, especially in the biofertilizer treatment. The yellow kernel had
the highest concentrations of yellow pigment, GSH, phytic phosphorous, Mg,
Mn, and Zn (19.61 µg g−1
, 1,134 nmol g−1
, 2.63 mg g−1
, 1,963 µg g−1
, 11.7 µg g−1
,
and 33.9 µg g−1
, respectively). The white kernel had a greater starch
concentration (2.5% higher than that in the red hybrid) and the potential
bioavailability of essential metals, particularly under no fertilization. This
supports the significance of white maize as a staple food in many traditional
diets across the world. Urea was important for the enhancement of the
antioxidant status (with 88.0% reduction capacity for the DPPH radical) and
increased potential Zn bioavailability in the maize kernels (13.3% higher than
that in the biofertilizer treatment). This study underlines the differences in the
yield potential and chemical composition of red, yellow, and white-kernel
maize and their importance as a necessary part of a sustainable human diet.
This information can help determine the most appropriate genotype based on
the antioxidants and/or essential elements targeted for kernel improvement.
PB  - Lausanne : Frontiers
T2  - Frontiers in plant science
T1  - Kernel color and fertilization as factors of enhanced maize quality
SP  - 1
EP  - 13
DO  - 10.3389/fpls.2022.1027618
ER  - 
@article{
author = "Dragičević, Vesna and Brankov, Milan and Stoiljkovic, Milovan and Tolimir, Miodrag and Travlos, Ilias and Simić, Milena",
year = "2022",
abstract = "Maize is an important staple crop and a significant source of various nutrients.
We aimed to determine the macronutrients, antioxidants, and essential
elements in maize genotypes (white, yellow, and red kernel) using three
different fertilizers, which could be used as a basis to increase the nutrient
density of maize. The fertilizer treatments used bio- and organic fertilizers as a
sustainable approach, urea, as a commonly used mineral fertilizer, and the
control (no fertilization). We evaluated the yield, concentration of
macronutrient (protein, oil, and starch), nonenzymatic antioxidants
(phenolics, yellow pigment, total glutathione (GSH), and phytic phosphorus),
and reduction capacity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, as
well as essential elements that are commonly deficient in the diet (Mg, Ca, Fe,
Mn, Zn, Cu, and S) and their relationships with phytic acid. The genotype
expressed the strongest effect on the variability of grain yield and the analyzed
grain constituents. The red-kernel hybrid showed the greatest accumulation of
protein, oil, phenolics, and essential elements (Ca, Fe, Cu, and S) than a yellow
and white hybrid, especially in the biofertilizer treatment. The yellow kernel had
the highest concentrations of yellow pigment, GSH, phytic phosphorous, Mg,
Mn, and Zn (19.61 µg g−1
, 1,134 nmol g−1
, 2.63 mg g−1
, 1,963 µg g−1
, 11.7 µg g−1
,
and 33.9 µg g−1
, respectively). The white kernel had a greater starch
concentration (2.5% higher than that in the red hybrid) and the potential
bioavailability of essential metals, particularly under no fertilization. This
supports the significance of white maize as a staple food in many traditional
diets across the world. Urea was important for the enhancement of the
antioxidant status (with 88.0% reduction capacity for the DPPH radical) and
increased potential Zn bioavailability in the maize kernels (13.3% higher than
that in the biofertilizer treatment). This study underlines the differences in the
yield potential and chemical composition of red, yellow, and white-kernel
maize and their importance as a necessary part of a sustainable human diet.
This information can help determine the most appropriate genotype based on
the antioxidants and/or essential elements targeted for kernel improvement.",
publisher = "Lausanne : Frontiers",
journal = "Frontiers in plant science",
title = "Kernel color and fertilization as factors of enhanced maize quality",
pages = "1-13",
doi = "10.3389/fpls.2022.1027618"
}
Dragičević, V., Brankov, M., Stoiljkovic, M., Tolimir, M., Travlos, I.,& Simić, M.. (2022). Kernel color and fertilization as factors of enhanced maize quality. in Frontiers in plant science
Lausanne : Frontiers., 1-13.
https://doi.org/10.3389/fpls.2022.1027618
Dragičević V, Brankov M, Stoiljkovic M, Tolimir M, Travlos I, Simić M. Kernel color and fertilization as factors of enhanced maize quality. in Frontiers in plant science. 2022;:1-13.
doi:10.3389/fpls.2022.1027618 .
Dragičević, Vesna, Brankov, Milan, Stoiljkovic, Milovan, Tolimir, Miodrag, Travlos, Ilias, Simić, Milena, "Kernel color and fertilization as factors of enhanced maize quality" in Frontiers in plant science (2022):1-13,
https://doi.org/10.3389/fpls.2022.1027618 . .

Sustainable fertilization systems as a prerequisite for improved quality of agricultural products

Dragičević, Vesna; Brankov, Milan; Stoiljkovic, Milovan; Šenk, Milena; Dolijanović, Željko; Tolimir, Miodrag; Simić, Milena

(Belgrade : University of Belgrade, Faculty of agriculture, 2022)

TY  - CONF
AU  - Dragičević, Vesna
AU  - Brankov, Milan
AU  - Stoiljkovic, Milovan
AU  - Šenk, Milena
AU  - Dolijanović, Željko
AU  - Tolimir, Miodrag
AU  - Simić, Milena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1056
AB  - Human health is dependent not just on diet, but mainly on quality of agricultural products as a 
part of diet. If crops were grown on poorly fertile soils, or they are exposed to severe stresses, 
lesser amount of mineral elements, particularly essential elements, such as zinc, copper, 
manganese, magnesium, calcium, iron, and even sulphur, will be absorbed and accumulated, 
resulting in their deficiency in diets and increased incidence of various chronic diseases. 
Together with naturally low soil fertility, climate change, intensive agriculture is one of the 
main contributors of soil depletion. Thus, various long-term strategies, which are sustainable 
for agricultural plants and soils, at the same time, must be developed. It is of particular 
importance to increase a level of organic matter, as a source of mineral nutrients from the soil. 
The application through soil, as well as via plant foliage of various complex and organic 
fertilizers, containing macro- and micro-elements, and many stimulating compounds, enables 
better absorption and metabolisation of nutrients required for plants and nutrients essential for 
humans. Besides, bio-fertilizers, containing beneficial microorganisms have an important role 
in nutrients mobilization in soils, particularly from poorly accessible forms. Many bio fertilizers contain microorganisms that are able to absorb atmospheric nitrogen, thus enriching 
soil, delivering it to the plants, enabling reduction in amount and costs of nitrogen addition 
into the soil. Promoting microorganisms are also able to enhance plants ability to absorb 
water and nutrients by their synergy with roots, resulting in stable and better growth 
performances of agricultural plants, thus increasing yield and its quality. Some other cropping 
practices, such as crop rotation, intercropping and use of cover crops, enriches soil with 
organic matter, reduces losses of nutrients through recycling of harvest residues, therefore 
increasing soil fertility, as well as quantity and quality of crop yield, at the same time.
PB  - Belgrade : University of Belgrade, Faculty of agriculture
C3  - 1. European symposium on phytochemicals in medicine and food, Belgrade, 7-9.09.2022. godine -  Book of abstracts
T1  - Sustainable fertilization systems as a prerequisite for  improved quality of agricultural products
SP  - 32
EP  - 32
ER  - 
@conference{
author = "Dragičević, Vesna and Brankov, Milan and Stoiljkovic, Milovan and Šenk, Milena and Dolijanović, Željko and Tolimir, Miodrag and Simić, Milena",
year = "2022",
abstract = "Human health is dependent not just on diet, but mainly on quality of agricultural products as a 
part of diet. If crops were grown on poorly fertile soils, or they are exposed to severe stresses, 
lesser amount of mineral elements, particularly essential elements, such as zinc, copper, 
manganese, magnesium, calcium, iron, and even sulphur, will be absorbed and accumulated, 
resulting in their deficiency in diets and increased incidence of various chronic diseases. 
Together with naturally low soil fertility, climate change, intensive agriculture is one of the 
main contributors of soil depletion. Thus, various long-term strategies, which are sustainable 
for agricultural plants and soils, at the same time, must be developed. It is of particular 
importance to increase a level of organic matter, as a source of mineral nutrients from the soil. 
The application through soil, as well as via plant foliage of various complex and organic 
fertilizers, containing macro- and micro-elements, and many stimulating compounds, enables 
better absorption and metabolisation of nutrients required for plants and nutrients essential for 
humans. Besides, bio-fertilizers, containing beneficial microorganisms have an important role 
in nutrients mobilization in soils, particularly from poorly accessible forms. Many bio fertilizers contain microorganisms that are able to absorb atmospheric nitrogen, thus enriching 
soil, delivering it to the plants, enabling reduction in amount and costs of nitrogen addition 
into the soil. Promoting microorganisms are also able to enhance plants ability to absorb 
water and nutrients by their synergy with roots, resulting in stable and better growth 
performances of agricultural plants, thus increasing yield and its quality. Some other cropping 
practices, such as crop rotation, intercropping and use of cover crops, enriches soil with 
organic matter, reduces losses of nutrients through recycling of harvest residues, therefore 
increasing soil fertility, as well as quantity and quality of crop yield, at the same time.",
publisher = "Belgrade : University of Belgrade, Faculty of agriculture",
journal = "1. European symposium on phytochemicals in medicine and food, Belgrade, 7-9.09.2022. godine -  Book of abstracts",
title = "Sustainable fertilization systems as a prerequisite for  improved quality of agricultural products",
pages = "32-32"
}
Dragičević, V., Brankov, M., Stoiljkovic, M., Šenk, M., Dolijanović, Ž., Tolimir, M.,& Simić, M.. (2022). Sustainable fertilization systems as a prerequisite for  improved quality of agricultural products. in 1. European symposium on phytochemicals in medicine and food, Belgrade, 7-9.09.2022. godine -  Book of abstracts
Belgrade : University of Belgrade, Faculty of agriculture., 32-32.
Dragičević V, Brankov M, Stoiljkovic M, Šenk M, Dolijanović Ž, Tolimir M, Simić M. Sustainable fertilization systems as a prerequisite for  improved quality of agricultural products. in 1. European symposium on phytochemicals in medicine and food, Belgrade, 7-9.09.2022. godine -  Book of abstracts. 2022;:32-32..
Dragičević, Vesna, Brankov, Milan, Stoiljkovic, Milovan, Šenk, Milena, Dolijanović, Željko, Tolimir, Miodrag, Simić, Milena, "Sustainable fertilization systems as a prerequisite for  improved quality of agricultural products" in 1. European symposium on phytochemicals in medicine and food, Belgrade, 7-9.09.2022. godine -  Book of abstracts (2022):32-32.

Properties of maize hybrid grain for utilization in wet milling

Nikolić, Valentina; Milašinović Šeremešić, Marija; Radosavljević, Milica; Simić, Marijana; Žilić, Slađana

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2022)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Milašinović Šeremešić, Marija
AU  - Radosavljević, Milica
AU  - Simić, Marijana
AU  - Žilić, Slađana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/988
AB  - A grain of eight selected hybrids from the Maize Research Institute Zemun Polje was used as
the subject of this study. The kernel structure, physical properties and chemical composition of
the grain and starch (amylose content) were analyzed before the laboratory wet milling process for
starch extraction. The in vitro enzymatic digestibility of the isolated starch was also determined.
The wet milling properties were later correlated to the physicochemical characteristics of the
grain. The results showed that hybrid ZP 677 had the highest starch yield, followed by hybrid ZP
704wx, while the highest starch recovery was observed for hybrid ZP 341. The highest bran yield
(10.52%) was obtained by hybrid ZP 434, and the highest germ yield (8.86%) by hybrid ZP 172/8.
The correlation analysis showed that starch yield was positively correlated to 1000-kernel weight
and crude fiber content, while oil content negatively influenced starch extraction and yield. The
starch digestibility was negatively correlated to the amylose content. These findings can be useful
for the starch processing industry as well as in the production of ethanol and alcoholic drinks.
AB  - Kao predmet ovog istraživanja korišćeno je zrno osam odabranih hibrida Instituta za kukuruz
„Zemun Polje”. Struktura zrna, fizička svojstva i hemijski sastav zrna i skroba (sadržaj amiloze)
analizirani su primenom laboratorijskog procesa mokrog mlevenja za izolovanje skroba. Takođe
je određena in vitro enzimska svarljivost izolovanog skroba. Svojstva mokrog mlevenja su potom
korelisana sa fizičko-hemijskim karakteristikama zrna. Rezultati su pokazali da je najviši prinos
skroba imao hibrid ZP 677, a zatim hibrid ZP 704wx, dok je najviša iskoristljivost skroba uočena
kod hibrida ZP 341. Najviši prinos mekinja (10,52%) dobijen je kod hibrida ZP 434, a najviši prinos klice (8,86%) utvrđen je kod hibrida ZP 172/8. Korelaciona analiza je pokazala da je prinos
skroba bio u pozitivnoj vezi sa apsolutnom masom zrna i sadržajem sirovih vlakana, dok je sadržaj ulja negativno uticao na izolaciju skroba i njegov prinos. Svarljivost skroba je bila u negativnoj
korelaciji sa sadržajem amiloze. Ova saznanja mogu biti korisna za industriju prerade skroba, kao
i za proizvodnju etanola i alkoholnih pića.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
PB  - Serbian Association of Plant Breedings and Seed Producers, Belgrade
T2  - Selekcija i semenarstvo
T1  - Properties of maize hybrid grain for utilization in wet milling
T1  - Svojstva zrna hibrida kukuruza za primenu u mokrom mlevenju
VL  - 28
IS  - 1
SP  - 67
EP  - 76
DO  - 10.5937/SelSem2201067N
ER  - 
@article{
author = "Nikolić, Valentina and Milašinović Šeremešić, Marija and Radosavljević, Milica and Simić, Marijana and Žilić, Slađana",
year = "2022",
abstract = "A grain of eight selected hybrids from the Maize Research Institute Zemun Polje was used as
the subject of this study. The kernel structure, physical properties and chemical composition of
the grain and starch (amylose content) were analyzed before the laboratory wet milling process for
starch extraction. The in vitro enzymatic digestibility of the isolated starch was also determined.
The wet milling properties were later correlated to the physicochemical characteristics of the
grain. The results showed that hybrid ZP 677 had the highest starch yield, followed by hybrid ZP
704wx, while the highest starch recovery was observed for hybrid ZP 341. The highest bran yield
(10.52%) was obtained by hybrid ZP 434, and the highest germ yield (8.86%) by hybrid ZP 172/8.
The correlation analysis showed that starch yield was positively correlated to 1000-kernel weight
and crude fiber content, while oil content negatively influenced starch extraction and yield. The
starch digestibility was negatively correlated to the amylose content. These findings can be useful
for the starch processing industry as well as in the production of ethanol and alcoholic drinks., Kao predmet ovog istraživanja korišćeno je zrno osam odabranih hibrida Instituta za kukuruz
„Zemun Polje”. Struktura zrna, fizička svojstva i hemijski sastav zrna i skroba (sadržaj amiloze)
analizirani su primenom laboratorijskog procesa mokrog mlevenja za izolovanje skroba. Takođe
je određena in vitro enzimska svarljivost izolovanog skroba. Svojstva mokrog mlevenja su potom
korelisana sa fizičko-hemijskim karakteristikama zrna. Rezultati su pokazali da je najviši prinos
skroba imao hibrid ZP 677, a zatim hibrid ZP 704wx, dok je najviša iskoristljivost skroba uočena
kod hibrida ZP 341. Najviši prinos mekinja (10,52%) dobijen je kod hibrida ZP 434, a najviši prinos klice (8,86%) utvrđen je kod hibrida ZP 172/8. Korelaciona analiza je pokazala da je prinos
skroba bio u pozitivnoj vezi sa apsolutnom masom zrna i sadržajem sirovih vlakana, dok je sadržaj ulja negativno uticao na izolaciju skroba i njegov prinos. Svarljivost skroba je bila u negativnoj
korelaciji sa sadržajem amiloze. Ova saznanja mogu biti korisna za industriju prerade skroba, kao
i za proizvodnju etanola i alkoholnih pića.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd, Serbian Association of Plant Breedings and Seed Producers, Belgrade",
journal = "Selekcija i semenarstvo",
title = "Properties of maize hybrid grain for utilization in wet milling, Svojstva zrna hibrida kukuruza za primenu u mokrom mlevenju",
volume = "28",
number = "1",
pages = "67-76",
doi = "10.5937/SelSem2201067N"
}
Nikolić, V., Milašinović Šeremešić, M., Radosavljević, M., Simić, M.,& Žilić, S.. (2022). Properties of maize hybrid grain for utilization in wet milling. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 28(1), 67-76.
https://doi.org/10.5937/SelSem2201067N
Nikolić V, Milašinović Šeremešić M, Radosavljević M, Simić M, Žilić S. Properties of maize hybrid grain for utilization in wet milling. in Selekcija i semenarstvo. 2022;28(1):67-76.
doi:10.5937/SelSem2201067N .
Nikolić, Valentina, Milašinović Šeremešić, Marija, Radosavljević, Milica, Simić, Marijana, Žilić, Slađana, "Properties of maize hybrid grain for utilization in wet milling" in Selekcija i semenarstvo, 28, no. 1 (2022):67-76,
https://doi.org/10.5937/SelSem2201067N . .

The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research

Nikolić, Valentina; Babić, Vojka; Kravić, Natalija; Filipović, Milomir; Žilić, Slađana; Simić, Marijana; Radosavljević, Milica

(Belgrade : Serbian Association of Plant Breedings and Seed Producers, 2022)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Babić, Vojka
AU  - Kravić, Natalija
AU  - Filipović, Milomir
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Radosavljević, Milica
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/849
AB  - In the process of hybrid maize breeding, the greatest attention is directed towards increasing the level and stability of the yield, while grain quality is of secondary importance. The very
principles of hybrid breeding, as well as the economic aspects and competition between different breeding companies, have led to an enormous narrowing of the genetic basis of commercial
maize hybrids. Concurrently, there is usually a small number of often related hybrids of similar
technological and nutritional quality present on the market. Six best-selling ZP maize hybrids
in the previous ten years were chosen as the subject of this study. In the period from 2009-2018,
both the yield components and parameters of technological and nutritional quality of the grain
were studied. It was found that the examined parameters varied a lot in different years of production and that some parameters were more influenced by genotype (number of kernel rows per ear,
portion of pericarp, and crude fiber content) while others were more influenced by the environment (kernel weight per ear, total protein, starch, and oil content, respectively). The amount and
the distribution of precipitation during the vegetative period affected not only the yield components i.e. grain yield but also the technological qualitz parametres and nutritional properties of
the grain. Based on the observed parental components, it is evident that they are related hybrids
that, despite the high genotype by environment interactions, consequently have similar parameters of technological quality and nutritional composition. The imperative is to develop special
breeding programs aiming to create both the starting material and parental components to provide hybrids of improved technological quality and nutritional properties on the market. In that
way, the needs of the processing industry for raw materials of certain characteristics can be met.
AB  - U procesu hibridnog oplemenjivanja kukuruza, najviše pažnje se posvećuje povećanju nivoa
i stabilnosti prinosa, dok je kvalitet zrna od sekundarnog značaja. Sami principi hibridnog oplemenjivanja, kao i ekonomski aspekti i konkurencija između različitih oplemenjivačkih kuća,
doveli su do enormnog suženja genetičke osnove komercijalnih hibrida kukuruza. Na tržištu je
istovremeno najčešće prisutan mali broj, često srodnih hibrida sličnog tehnološkog i nutritivnog
kvaliteta. Za ovo istraživanje je odabrano šest ZP hibrida kukuruza najprodavanijih u prethodnih
deset godina. U periodu od 2009-2018. godine, praćene su, kako komponente prinosa, tako i
parametri tehnološkog i nutritivnog kvaliteta zrna. Utvrđeno je da su ispitivani parametri veoma
varirali u različitim godinama gajenja, kao i da su neki parametri bili pod većim uticajem genotipa (broj redova zrna, udeo perikarpa i sirove celuloze), a neki pod većim uticajem spoljašnje sredine (masa zrna po klipu, udeo ukupnih proteina, skroba i ulja, respektivno). Količina i raspored
padavina u toku vegetativnog perioda su uticali ne samo na komponente prinosa, odnosno na
prinos zrna, već i na parametre tehnološkog kvaliteta i nutritivna svojstva. Na osnovu posmatranih roditeljskih komponenata, evidentno je da se radi o srodnim hibridima koji, bez obzira na
visoke interakcije između genotipa i spoljašnje sredine, posledično imaju slične parametre tehnološkog kvaliteta kao i nutritivni sastav. Neophodno je razvijati posebne oplemenjivačke programe
koji imaju za cilj stvaranje kako početnog materijala, tako i roditeljskih komponenata, kako bi
se na tržištu obezbedili hibridi poboljšanog tehnološkog kvaliteta i nutritivnih svojstava i na taj
način zadovoljile potrebe prerađivačke industrije za sirovinama određenih karakteristika.
PB  - Belgrade : Serbian Association of Plant Breedings and Seed Producers
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research
T1  - Varijabilnost i međuzavisnost osnovnih parametara tehnološkog kvaliteta hibrida kukuruza u dugoročnim istraživanjima
VL  - 27
IS  - 2
SP  - 21
EP  - 33
DO  - 10.5937/SelSem2102021N
ER  - 
@article{
author = "Nikolić, Valentina and Babić, Vojka and Kravić, Natalija and Filipović, Milomir and Žilić, Slađana and Simić, Marijana and Radosavljević, Milica",
year = "2022",
abstract = "In the process of hybrid maize breeding, the greatest attention is directed towards increasing the level and stability of the yield, while grain quality is of secondary importance. The very
principles of hybrid breeding, as well as the economic aspects and competition between different breeding companies, have led to an enormous narrowing of the genetic basis of commercial
maize hybrids. Concurrently, there is usually a small number of often related hybrids of similar
technological and nutritional quality present on the market. Six best-selling ZP maize hybrids
in the previous ten years were chosen as the subject of this study. In the period from 2009-2018,
both the yield components and parameters of technological and nutritional quality of the grain
were studied. It was found that the examined parameters varied a lot in different years of production and that some parameters were more influenced by genotype (number of kernel rows per ear,
portion of pericarp, and crude fiber content) while others were more influenced by the environment (kernel weight per ear, total protein, starch, and oil content, respectively). The amount and
the distribution of precipitation during the vegetative period affected not only the yield components i.e. grain yield but also the technological qualitz parametres and nutritional properties of
the grain. Based on the observed parental components, it is evident that they are related hybrids
that, despite the high genotype by environment interactions, consequently have similar parameters of technological quality and nutritional composition. The imperative is to develop special
breeding programs aiming to create both the starting material and parental components to provide hybrids of improved technological quality and nutritional properties on the market. In that
way, the needs of the processing industry for raw materials of certain characteristics can be met., U procesu hibridnog oplemenjivanja kukuruza, najviše pažnje se posvećuje povećanju nivoa
i stabilnosti prinosa, dok je kvalitet zrna od sekundarnog značaja. Sami principi hibridnog oplemenjivanja, kao i ekonomski aspekti i konkurencija između različitih oplemenjivačkih kuća,
doveli su do enormnog suženja genetičke osnove komercijalnih hibrida kukuruza. Na tržištu je
istovremeno najčešće prisutan mali broj, često srodnih hibrida sličnog tehnološkog i nutritivnog
kvaliteta. Za ovo istraživanje je odabrano šest ZP hibrida kukuruza najprodavanijih u prethodnih
deset godina. U periodu od 2009-2018. godine, praćene su, kako komponente prinosa, tako i
parametri tehnološkog i nutritivnog kvaliteta zrna. Utvrđeno je da su ispitivani parametri veoma
varirali u različitim godinama gajenja, kao i da su neki parametri bili pod većim uticajem genotipa (broj redova zrna, udeo perikarpa i sirove celuloze), a neki pod većim uticajem spoljašnje sredine (masa zrna po klipu, udeo ukupnih proteina, skroba i ulja, respektivno). Količina i raspored
padavina u toku vegetativnog perioda su uticali ne samo na komponente prinosa, odnosno na
prinos zrna, već i na parametre tehnološkog kvaliteta i nutritivna svojstva. Na osnovu posmatranih roditeljskih komponenata, evidentno je da se radi o srodnim hibridima koji, bez obzira na
visoke interakcije između genotipa i spoljašnje sredine, posledično imaju slične parametre tehnološkog kvaliteta kao i nutritivni sastav. Neophodno je razvijati posebne oplemenjivačke programe
koji imaju za cilj stvaranje kako početnog materijala, tako i roditeljskih komponenata, kako bi
se na tržištu obezbedili hibridi poboljšanog tehnološkog kvaliteta i nutritivnih svojstava i na taj
način zadovoljile potrebe prerađivačke industrije za sirovinama određenih karakteristika.",
publisher = "Belgrade : Serbian Association of Plant Breedings and Seed Producers, Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research, Varijabilnost i međuzavisnost osnovnih parametara tehnološkog kvaliteta hibrida kukuruza u dugoročnim istraživanjima",
volume = "27",
number = "2",
pages = "21-33",
doi = "10.5937/SelSem2102021N"
}
Nikolić, V., Babić, V., Kravić, N., Filipović, M., Žilić, S., Simić, M.,& Radosavljević, M.. (2022). The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research. in Selekcija i semenarstvo
Belgrade : Serbian Association of Plant Breedings and Seed Producers., 27(2), 21-33.
https://doi.org/10.5937/SelSem2102021N
Nikolić V, Babić V, Kravić N, Filipović M, Žilić S, Simić M, Radosavljević M. The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research. in Selekcija i semenarstvo. 2022;27(2):21-33.
doi:10.5937/SelSem2102021N .
Nikolić, Valentina, Babić, Vojka, Kravić, Natalija, Filipović, Milomir, Žilić, Slađana, Simić, Marijana, Radosavljević, Milica, "The variability and interdependence of basic technological quality parameters of maize hybrids in long-term research" in Selekcija i semenarstvo, 27, no. 2 (2022):21-33,
https://doi.org/10.5937/SelSem2102021N . .

Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study

Bartolić, Dragana; Mutavdzic, Dragosav; Carstensen, Jens Michael; Stanković, Slavica; Nikolić, Milica; Krstović, Saša; Radotić, Ksenija

(Berlin : Springer Nature, 2022)

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mutavdzic, Dragosav
AU  - Carstensen, Jens Michael
AU  - Stanković, Slavica
AU  - Nikolić, Milica
AU  - Krstović, Saša
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/838
AB  - Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non‑invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B 1 (AFB 1 ) from the uncontaminated seeds. The AFB 1‑contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB 1‑contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB 1‑contaminated seeds showed a 7.9 to 9.6‑fold increase in the seed
reflectance in the VIS region, and 10.4 and 12.2‑fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non‑invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.
PB  - Berlin : Springer Nature
T2  - Scientific Reports
T1  - Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study
VL  - 12
SP  - 4849
DO  - 10.1038/s41598-022-08352-4
ER  - 
@article{
author = "Bartolić, Dragana and Mutavdzic, Dragosav and Carstensen, Jens Michael and Stanković, Slavica and Nikolić, Milica and Krstović, Saša and Radotić, Ksenija",
year = "2022",
abstract = "Cereal seeds safety may be compromised by the presence of toxic contaminants, such as aflatoxins.
Besides being carcinogenic, they have other adverse health effects on humans and animals. In
this preliminary study, we used two non‑invasive optical techniques, optical fiber fluorescence
spectroscopy and multispectral imaging (MSI), for discrimination of maize seeds naturally
contaminated with aflatoxin B 1 (AFB 1 ) from the uncontaminated seeds. The AFB 1‑contaminated
seeds exhibited a red shift of the emission maximum position compared to the control samples.
Using linear discrimination analysis to analyse fluorescence data, classification accuracy of 100%
was obtained to discriminate uncontaminated and AFB 1‑contaminated seeds. The MSI analysis
combined with a normalized canonical discriminant analysis, provided spectral and spatial patterns
of the analysed seeds. The AFB 1‑contaminated seeds showed a 7.9 to 9.6‑fold increase in the seed
reflectance in the VIS region, and 10.4 and 12.2‑fold increase in the NIR spectral region, compared
with the uncontaminated seeds. Thus the MSI method classified successfully contaminated from
uncontaminated seeds with high accuracy. The results may have an impact on development of
spectroscopic non‑invasive methods for detection of AFs presence in seeds, providing valuable
information for the assessment of seed adulteration in the field of food forensics and food safety.",
publisher = "Berlin : Springer Nature",
journal = "Scientific Reports",
title = "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study",
volume = "12",
pages = "4849",
doi = "10.1038/s41598-022-08352-4"
}
Bartolić, D., Mutavdzic, D., Carstensen, J. M., Stanković, S., Nikolić, M., Krstović, S.,& Radotić, K.. (2022). Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports
Berlin : Springer Nature., 12, 4849.
https://doi.org/10.1038/s41598-022-08352-4
Bartolić D, Mutavdzic D, Carstensen JM, Stanković S, Nikolić M, Krstović S, Radotić K. Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study. in Scientific Reports. 2022;12:4849.
doi:10.1038/s41598-022-08352-4 .
Bartolić, Dragana, Mutavdzic, Dragosav, Carstensen, Jens Michael, Stanković, Slavica, Nikolić, Milica, Krstović, Saša, Radotić, Ksenija, "Fluorescence spectroscopy and multispectral imaging for fingerprinting of aflatoxin‑B1 contaminated (Zea mays L.) seeds: a preliminary study" in Scientific Reports, 12 (2022):4849,
https://doi.org/10.1038/s41598-022-08352-4 . .
1
2

Nutritive quality and plant digestibility of silage maize hybrids from Serbia

Nikolić, Valentina; Simić, Marijana; Žilić, Slađana

(Kiev : Ukrainian Institute for Plant Variety Examination, 2022)

TY  - CONF
AU  - Nikolić, Valentina
AU  - Simić, Marijana
AU  - Žilić, Slađana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/898
AB  - The quality of five silage maize hybrids grown
in 2020 at five different locations in Serbia (two in
the northern province of Vojvodina (Srem) and three
in Central Serbia) was tested in 2021 in the laboratory
of the Department of Food Technology and
Biochemistry of the Maize Research Institute “Zemun
Polje“. The dry matter content, lignocellulose
fiber content, and in vitro dry matter digestibility
(IVDMD) of of the whole plant maize hybrids: ZP
707, ZP 7357, ZP 790, ZP 8701, and ZP 873 were
investigated. The 7001 hybrid was used as a standard.
The average dry matter content varied between
37.77±1.88% (ZP 790) and 42.64±7.19% (ZP
707), while in vitro dry matter digestibility ranged
from 58.77±1.96% (ZP 8701) to 63.77±2.09% (ZP
707). The share, as well as the type of the plant
cell wall components – lignocellulosic fibers, determines
the nutritional quality of silage maize
as animal feed. The NDF content ranged from As
the NDF content increases with maize maturity,
animals show a tendency for lower silage intake.
However, although in some cases NDF can be considered
a negative indicator of silage quality, NDF
is a necessary component of ruminant nutrition.
Based on the achieved results, it can be concluded
that hybrids ZP 707 and ZP 7357 proved to be the
best silage maize forms. At most locations, these
hybrids achieved better results than the standard.
The ZP 707 hybrid on average had the highest dry
matter content, the highest digestibility of dry
matter, as well as the lowest content of all lignocellulosic
fibers, which all indicate its potential
as silage maize form. The lack of hybrids ZP 707
and ZP 7357 can be considered the fact that the
dry matter content of the whole plant showed to be
less stable in different agro-ecological conditions.In terms of digestibility and dry matter content,
the ZP 790 hybrid can be singled out as the most
stable. The ZP 790 hybrid is less recommended for
Srem, but in Central Serbia, it could match the ZP
707 and ZP 7357 hybrids. ZP 8701 proved to be the
weakest hybrid at all locations, followed by ZP 873.
The in vitro digestibility and dry matter content
of the whole plant of the investigated hybrids were
far more stable in Srem than in Central Serbia.
These findings can be of great importance for future
breeding programs directed toward creating
new and improved silage maize hybrids.
PB  - Kiev : Ukrainian Institute for Plant Variety Examination
C3  - 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings
T1  - Nutritive quality and plant digestibility of silage maize hybrids from Serbia
SP  - 78
EP  - 79
ER  - 
@conference{
author = "Nikolić, Valentina and Simić, Marijana and Žilić, Slađana",
year = "2022",
abstract = "The quality of five silage maize hybrids grown
in 2020 at five different locations in Serbia (two in
the northern province of Vojvodina (Srem) and three
in Central Serbia) was tested in 2021 in the laboratory
of the Department of Food Technology and
Biochemistry of the Maize Research Institute “Zemun
Polje“. The dry matter content, lignocellulose
fiber content, and in vitro dry matter digestibility
(IVDMD) of of the whole plant maize hybrids: ZP
707, ZP 7357, ZP 790, ZP 8701, and ZP 873 were
investigated. The 7001 hybrid was used as a standard.
The average dry matter content varied between
37.77±1.88% (ZP 790) and 42.64±7.19% (ZP
707), while in vitro dry matter digestibility ranged
from 58.77±1.96% (ZP 8701) to 63.77±2.09% (ZP
707). The share, as well as the type of the plant
cell wall components – lignocellulosic fibers, determines
the nutritional quality of silage maize
as animal feed. The NDF content ranged from As
the NDF content increases with maize maturity,
animals show a tendency for lower silage intake.
However, although in some cases NDF can be considered
a negative indicator of silage quality, NDF
is a necessary component of ruminant nutrition.
Based on the achieved results, it can be concluded
that hybrids ZP 707 and ZP 7357 proved to be the
best silage maize forms. At most locations, these
hybrids achieved better results than the standard.
The ZP 707 hybrid on average had the highest dry
matter content, the highest digestibility of dry
matter, as well as the lowest content of all lignocellulosic
fibers, which all indicate its potential
as silage maize form. The lack of hybrids ZP 707
and ZP 7357 can be considered the fact that the
dry matter content of the whole plant showed to be
less stable in different agro-ecological conditions.In terms of digestibility and dry matter content,
the ZP 790 hybrid can be singled out as the most
stable. The ZP 790 hybrid is less recommended for
Srem, but in Central Serbia, it could match the ZP
707 and ZP 7357 hybrids. ZP 8701 proved to be the
weakest hybrid at all locations, followed by ZP 873.
The in vitro digestibility and dry matter content
of the whole plant of the investigated hybrids were
far more stable in Srem than in Central Serbia.
These findings can be of great importance for future
breeding programs directed toward creating
new and improved silage maize hybrids.",
publisher = "Kiev : Ukrainian Institute for Plant Variety Examination",
journal = "10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings",
title = "Nutritive quality and plant digestibility of silage maize hybrids from Serbia",
pages = "78-79"
}
Nikolić, V., Simić, M.,& Žilić, S.. (2022). Nutritive quality and plant digestibility of silage maize hybrids from Serbia. in 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings
Kiev : Ukrainian Institute for Plant Variety Examination., 78-79.
Nikolić V, Simić M, Žilić S. Nutritive quality and plant digestibility of silage maize hybrids from Serbia. in 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings. 2022;:78-79..
Nikolić, Valentina, Simić, Marijana, Žilić, Slađana, "Nutritive quality and plant digestibility of silage maize hybrids from Serbia" in 10. International applied science conference of young scientists and experts, April 29, 2022, the village of Tsentralne, Kyiv region, Ukraine - Book of proceedings (2022):78-79.

Suitability of the selected local maize hybrids for silage production

Nikolić, Valentina; Simić, Marijana; Žilić, Slađana; Kravić, Natalija; Babić, Vojka; Filipović, Milomir; Srdić, Jelena

(Cacak : University of Kragujevac, Faculty of Agronomy, 2022)

TY  - CONF
AU  - Nikolić, Valentina
AU  - Simić, Marijana
AU  - Žilić, Slađana
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Filipović, Milomir
AU  - Srdić, Jelena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/874
AB  - The main goal of this study was to observe the properties of fifteen
different genotypes of maize hybrids from Serbia in order to determine their
suitability for the production of high-quality silage for ruminant feed. The research
was conducted in a two-year field experiment at the location of the Maize Research
Institute in Zemun Polje, Serbia, and the laboratory analyses included yield structure
of the investigated maize hybrids, assessment of the lignocellulosic fiber
composition, as well as the in vitro dry matter digestibility of the whole plant
samples. All maize hybrids have shown good quality traits that are a prerequisite for
the production of high-quality silage.
PB  - Cacak : University of Kragujevac, Faculty of Agronomy
C3  - 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova
T1  - Suitability of the selected local maize hybrids for silage production
SP  - 105
EP  - 110
DO  - 10.46793/SBT27.105N
ER  - 
@conference{
author = "Nikolić, Valentina and Simić, Marijana and Žilić, Slađana and Kravić, Natalija and Babić, Vojka and Filipović, Milomir and Srdić, Jelena",
year = "2022",
abstract = "The main goal of this study was to observe the properties of fifteen
different genotypes of maize hybrids from Serbia in order to determine their
suitability for the production of high-quality silage for ruminant feed. The research
was conducted in a two-year field experiment at the location of the Maize Research
Institute in Zemun Polje, Serbia, and the laboratory analyses included yield structure
of the investigated maize hybrids, assessment of the lignocellulosic fiber
composition, as well as the in vitro dry matter digestibility of the whole plant
samples. All maize hybrids have shown good quality traits that are a prerequisite for
the production of high-quality silage.",
publisher = "Cacak : University of Kragujevac, Faculty of Agronomy",
journal = "27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova",
title = "Suitability of the selected local maize hybrids for silage production",
pages = "105-110",
doi = "10.46793/SBT27.105N"
}
Nikolić, V., Simić, M., Žilić, S., Kravić, N., Babić, V., Filipović, M.,& Srdić, J.. (2022). Suitability of the selected local maize hybrids for silage production. in 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova
Cacak : University of Kragujevac, Faculty of Agronomy., 105-110.
https://doi.org/10.46793/SBT27.105N
Nikolić V, Simić M, Žilić S, Kravić N, Babić V, Filipović M, Srdić J. Suitability of the selected local maize hybrids for silage production. in 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova. 2022;:105-110.
doi:10.46793/SBT27.105N .
Nikolić, Valentina, Simić, Marijana, Žilić, Slađana, Kravić, Natalija, Babić, Vojka, Filipović, Milomir, Srdić, Jelena, "Suitability of the selected local maize hybrids for silage production" in 27. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 25 -26. mart 2022. godine - Zbornik radova (2022):105-110,
https://doi.org/10.46793/SBT27.105N . .

Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Perić, Vesna; Srdić, Jelena; Vasić, Marko G.

(Novi Sad : National Society of Processing and Energy in Agriculture, 2022)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Perić, Vesna
AU  - Srdić, Jelena
AU  - Vasić, Marko G.
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/847
AB  - Black  soybean  seed  coat  has  a  considerable  content  of  anthocyanins  which  can  providea  positive  effect  on  the  health  of  the  consumers through food products enriched with the extracts of these valuable bioactive compounds. Nevertheless, the use of soybean seed  coat,  a  by-product  from  soybean  processing,  additionally  valorizes  this  raw  material.The  possibility  of  enrichment  of  sweet maize  grains  with  anthocyanin  extract  was  investigated.  Several  procedures  with  acetic  acid  were  applied  in  the  experiments  of  anthocyanins  extraction  from  black  soybean  seed  coat.  During  the  marination  process,  the  grains  were  stained,  changing  their  standard yellow color to a crimson red. Since the brines with the addition of citric acid became cloudy (opalescent) after some time, lactic  acid  was  used  as  an  anthocyanin  stabilizer  in  the  continuation  of  the  research.  Depending  on  the  extraction  conditions,  the spectrophotometrically determined content of total anthocyanins in the solution varied from 3541.90 to 5387.70 μg CGE/g d.m., and in the marinated maize grain between 179.89 and 286.05 μg CGE/g d.m. After seven days, the total anthocyanin content in the grain did not increase significantly, so this aging period was selected as optimal for marinating maize products.
AB  - Semenjača  crne  soje  ima  značajan  sadržaj  antocijana,  koji  mogu  pozitivno  uticati  na  zdravlje  potrošača  kroz  prehrambene  proizvode  obogaćene  ekstraktima  ovih  vrednih  bioaktivnih  jedinjenja.  Istovremeno,  upotrebom  sojine  semenjače,  sporednog  proizvoda prerade soje, dodatno se valorizuje ova sirovinu. Ispitivana je mogućnost obogaćivanja zrna kukuruza šećerca ekstraktom antocijana.  U  eksperimentima  ekstrakcije  antocijana  iz  semenjače  crne  soje  primenjeno  je  nekoliko  postupaka  sa  sirćetnom kiselinom.  Tokom  procesa  mariniranja,  zrna  su  promenila  boju,  menjajući  standardnu  žutu  boju  u  tamnocrvenu.  S  obzirom  da  su  posle izvesnog vremena nalivi za mariniranje sa dodatkom limunske kiseline postali zamućeni (opalescentni), u nastavku istraživanja je  korišćena  mlečna  kiselina  kao  stabilizator  antocijana.  U  zavisnosti  od  uslova  ekstrakcije,  spektrofotometrijski  utvrđen  sadržaj  ukupnih  antocijana  u  rastvoru  varirao  je  od  3541,90  do  5387,70  mg  CGE/g  d.m.,  a  u  zrnu  mariniranog  kukuruza  između  179,89  i  286,05  mg  CGE/g.  Posle  sedam  dana,  ukupan  sadržaj  antocijana  u  zrnu  nije  značajno  povećan,  pa  je  ovaj  period  odležavanja  izabran kao optimalan za mariniranje proizvoda od kukuruza.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food
T1  - Optimizacija procesa ekstrakcije antocijana iz semenjače crne soje za pripremu funkcionalne hrane od kukuruza
VL  - 26
IS  - 1
SP  - 19
EP  - 22
DO  - 10.5937/jpea%v-36635
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Perić, Vesna and Srdić, Jelena and Vasić, Marko G.",
year = "2022",
abstract = "Black  soybean  seed  coat  has  a  considerable  content  of  anthocyanins  which  can  providea  positive  effect  on  the  health  of  the  consumers through food products enriched with the extracts of these valuable bioactive compounds. Nevertheless, the use of soybean seed  coat,  a  by-product  from  soybean  processing,  additionally  valorizes  this  raw  material.The  possibility  of  enrichment  of  sweet maize  grains  with  anthocyanin  extract  was  investigated.  Several  procedures  with  acetic  acid  were  applied  in  the  experiments  of  anthocyanins  extraction  from  black  soybean  seed  coat.  During  the  marination  process,  the  grains  were  stained,  changing  their  standard yellow color to a crimson red. Since the brines with the addition of citric acid became cloudy (opalescent) after some time, lactic  acid  was  used  as  an  anthocyanin  stabilizer  in  the  continuation  of  the  research.  Depending  on  the  extraction  conditions,  the spectrophotometrically determined content of total anthocyanins in the solution varied from 3541.90 to 5387.70 μg CGE/g d.m., and in the marinated maize grain between 179.89 and 286.05 μg CGE/g d.m. After seven days, the total anthocyanin content in the grain did not increase significantly, so this aging period was selected as optimal for marinating maize products., Semenjača  crne  soje  ima  značajan  sadržaj  antocijana,  koji  mogu  pozitivno  uticati  na  zdravlje  potrošača  kroz  prehrambene  proizvode  obogaćene  ekstraktima  ovih  vrednih  bioaktivnih  jedinjenja.  Istovremeno,  upotrebom  sojine  semenjače,  sporednog  proizvoda prerade soje, dodatno se valorizuje ova sirovinu. Ispitivana je mogućnost obogaćivanja zrna kukuruza šećerca ekstraktom antocijana.  U  eksperimentima  ekstrakcije  antocijana  iz  semenjače  crne  soje  primenjeno  je  nekoliko  postupaka  sa  sirćetnom kiselinom.  Tokom  procesa  mariniranja,  zrna  su  promenila  boju,  menjajući  standardnu  žutu  boju  u  tamnocrvenu.  S  obzirom  da  su  posle izvesnog vremena nalivi za mariniranje sa dodatkom limunske kiseline postali zamućeni (opalescentni), u nastavku istraživanja je  korišćena  mlečna  kiselina  kao  stabilizator  antocijana.  U  zavisnosti  od  uslova  ekstrakcije,  spektrofotometrijski  utvrđen  sadržaj  ukupnih  antocijana  u  rastvoru  varirao  je  od  3541,90  do  5387,70  mg  CGE/g  d.m.,  a  u  zrnu  mariniranog  kukuruza  između  179,89  i  286,05  mg  CGE/g.  Posle  sedam  dana,  ukupan  sadržaj  antocijana  u  zrnu  nije  značajno  povećan,  pa  je  ovaj  period  odležavanja  izabran kao optimalan za mariniranje proizvoda od kukuruza.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food, Optimizacija procesa ekstrakcije antocijana iz semenjače crne soje za pripremu funkcionalne hrane od kukuruza",
volume = "26",
number = "1",
pages = "19-22",
doi = "10.5937/jpea%v-36635"
}
Nikolić, V., Žilić, S., Simić, M., Perić, V., Srdić, J.,& Vasić, M. G.. (2022). Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 26(1), 19-22.
https://doi.org/10.5937/jpea%v-36635
Nikolić V, Žilić S, Simić M, Perić V, Srdić J, Vasić MG. Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food. in Journal on Processing and Energy in Agriculture. 2022;26(1):19-22.
doi:10.5937/jpea%v-36635 .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Perić, Vesna, Srdić, Jelena, Vasić, Marko G., "Optimization of anthocyanins extraction process from black soybean seed coat for the preparation of maize-based functional food" in Journal on Processing and Energy in Agriculture, 26, no. 1 (2022):19-22,
https://doi.org/10.5937/jpea%v-36635 . .

Acrylamide in Corn-Based Thermally Processed Foods: A Review

Žilić, Slađana; Nikolić, Valentina; Mogol, Burce Atac; Hamzalioglu, Aytul; Tas, Neslihan Goncuoglu; Kocadagli, Tolgahan; Simić, Marijana; Gokmen, Vural

(Washington : ACS Publications, 2022)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Nikolić, Valentina
AU  - Mogol, Burce Atac
AU  - Hamzalioglu, Aytul
AU  - Tas, Neslihan Goncuoglu
AU  - Kocadagli, Tolgahan
AU  - Simić, Marijana
AU  - Gokmen, Vural
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/855
AB  - Widely consumed thermally processed corn-based foods can have a great contribution to acrylamide dietary intake,
thus bearing a high public health risk and requiring attention and application of strategies for its reduction. This paper reviews the
literature on the acrylamide content of corn-based food products present in the market around the world. The potential of corn for
acrylamide formation due to its content of free asparagine and reducing sugars is described. Human exposure to acrylamide from
corn-based foods is also discussed. The content of acrylamide in corn/tortilla chips, popcorn, and corn flakes, as widely consumed
products all over the world, is reported in the literature to be between 5 and 6360 μg/kg, between <LOD and 2220 μg/kg and
between <LOD and 1186 μg/kg, respectively. Although these products are important acrylamide sources in the common diet of all
age populations, higher intake values occurred among younger generations.
PB  - Washington : ACS Publications
T2  - Journal of agricultural and food chemistry
T1  - Acrylamide in Corn-Based Thermally Processed Foods: A Review
VL  - 70
SP  - 4165
EP  - 4181
DO  - 10.1021/acs.jafc.1c07249
ER  - 
@article{
author = "Žilić, Slađana and Nikolić, Valentina and Mogol, Burce Atac and Hamzalioglu, Aytul and Tas, Neslihan Goncuoglu and Kocadagli, Tolgahan and Simić, Marijana and Gokmen, Vural",
year = "2022",
abstract = "Widely consumed thermally processed corn-based foods can have a great contribution to acrylamide dietary intake,
thus bearing a high public health risk and requiring attention and application of strategies for its reduction. This paper reviews the
literature on the acrylamide content of corn-based food products present in the market around the world. The potential of corn for
acrylamide formation due to its content of free asparagine and reducing sugars is described. Human exposure to acrylamide from
corn-based foods is also discussed. The content of acrylamide in corn/tortilla chips, popcorn, and corn flakes, as widely consumed
products all over the world, is reported in the literature to be between 5 and 6360 μg/kg, between <LOD and 2220 μg/kg and
between <LOD and 1186 μg/kg, respectively. Although these products are important acrylamide sources in the common diet of all
age populations, higher intake values occurred among younger generations.",
publisher = "Washington : ACS Publications",
journal = "Journal of agricultural and food chemistry",
title = "Acrylamide in Corn-Based Thermally Processed Foods: A Review",
volume = "70",
pages = "4165-4181",
doi = "10.1021/acs.jafc.1c07249"
}
Žilić, S., Nikolić, V., Mogol, B. A., Hamzalioglu, A., Tas, N. G., Kocadagli, T., Simić, M.,& Gokmen, V.. (2022). Acrylamide in Corn-Based Thermally Processed Foods: A Review. in Journal of agricultural and food chemistry
Washington : ACS Publications., 70, 4165-4181.
https://doi.org/10.1021/acs.jafc.1c07249
Žilić S, Nikolić V, Mogol BA, Hamzalioglu A, Tas NG, Kocadagli T, Simić M, Gokmen V. Acrylamide in Corn-Based Thermally Processed Foods: A Review. in Journal of agricultural and food chemistry. 2022;70:4165-4181.
doi:10.1021/acs.jafc.1c07249 .
Žilić, Slađana, Nikolić, Valentina, Mogol, Burce Atac, Hamzalioglu, Aytul, Tas, Neslihan Goncuoglu, Kocadagli, Tolgahan, Simić, Marijana, Gokmen, Vural, "Acrylamide in Corn-Based Thermally Processed Foods: A Review" in Journal of agricultural and food chemistry, 70 (2022):4165-4181,
https://doi.org/10.1021/acs.jafc.1c07249 . .
3

Contribution of long-term crop rotation to weed control in maize

Simić, Milena; Brankov, Milan; Dragičević, Vesna

(2022)

TY  - CONF
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Dragičević, Vesna
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/997
AB  - Crop rotation is an essential part of IWM system proposed to be implemented in maize weed 
control. It is especially appropriate to be used in sustainable maize production in order to suppress weed abundance on ecological and low-pesticide way. In comparison to maize continuous cropping, which is not suggestible, two- and three-years crop rotation with legume crop 
is more efficient in weed biomass reduction, including basic herbicide application. The aim 
of the study was to investigate the efficacy of combined application of crop rotation and mixture of soil (PRE) herbicides on weed species distribution. A long-term field trial organized as split-plot experiment has started in 2009 with maize sown in all four fields at the Maize Research Institute, Belgrade, Serbia. The basic treatment was a rotation system: maize continuous cropping (MC), maize-winter wheat rotation (MW), maize-soybean - w. wheat (MSW) and maize - w. wheat - soybean rotation (MWS). A pre-emergence herbicide mixture of isoxaflutole and metolachlor (Merlin 750-WG+Dual Gold 960 EC) in recommended rates (105 g a.i. ha-1 + 672 g a.i. ha-1) was applied immediately after drilling with a CO2 backpack sprayer with a four nozzle boom, using extended range nozzles (XR11002-SS, Tee Jet Spraying Systems, Wheaton, IL, USA) calibrated to deliver a spray volume of 140 L ha-1 of solution at 275.8 kPa. Herbicides were not applied in the control plot. After twelve years and completion of four rotations, maize was present again in all fields in 2021. Weed infestation level was estimated six to seven weeks after the application of herbicides. Samples were drawn randomly by the one square meter and number of weed individuals (WI) and their biomass (WB) were calculated. The data were processed by ANOVA. Our results indicated that in comparison to 2009, WI were increased in 2021 in untreated control by 44.2% in MC, 23.6% in MW, 24.6% in MSW and 18.6% in MWS. On the other hand, in treated plots WI have increased only in MC, by 62.5% and lowered by 7.7% in MW, 29.4% in MSW and 60.0% in MWS. WB has been reduced in 2021 significantly on treated as well as untreated plots in all rotation systems. The highest reduction of WB was achieved in untreated control of MWS (42.6%) and treated plot in MC (45.6%). Conclusively, it was proved that even with basic herbicide treatment with soil herbicides crop rotation could be very effec tive in weed control. On the other hand, maize continuous cropping has to be avoided in order to reduce potentials for weed infestation.
C3  - 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts
T1  - Contribution of long-term crop rotation to weed control in maize
SP  - 215
EP  - 215
ER  - 
@conference{
author = "Simić, Milena and Brankov, Milan and Dragičević, Vesna",
year = "2022",
abstract = "Crop rotation is an essential part of IWM system proposed to be implemented in maize weed 
control. It is especially appropriate to be used in sustainable maize production in order to suppress weed abundance on ecological and low-pesticide way. In comparison to maize continuous cropping, which is not suggestible, two- and three-years crop rotation with legume crop 
is more efficient in weed biomass reduction, including basic herbicide application. The aim 
of the study was to investigate the efficacy of combined application of crop rotation and mixture of soil (PRE) herbicides on weed species distribution. A long-term field trial organized as split-plot experiment has started in 2009 with maize sown in all four fields at the Maize Research Institute, Belgrade, Serbia. The basic treatment was a rotation system: maize continuous cropping (MC), maize-winter wheat rotation (MW), maize-soybean - w. wheat (MSW) and maize - w. wheat - soybean rotation (MWS). A pre-emergence herbicide mixture of isoxaflutole and metolachlor (Merlin 750-WG+Dual Gold 960 EC) in recommended rates (105 g a.i. ha-1 + 672 g a.i. ha-1) was applied immediately after drilling with a CO2 backpack sprayer with a four nozzle boom, using extended range nozzles (XR11002-SS, Tee Jet Spraying Systems, Wheaton, IL, USA) calibrated to deliver a spray volume of 140 L ha-1 of solution at 275.8 kPa. Herbicides were not applied in the control plot. After twelve years and completion of four rotations, maize was present again in all fields in 2021. Weed infestation level was estimated six to seven weeks after the application of herbicides. Samples were drawn randomly by the one square meter and number of weed individuals (WI) and their biomass (WB) were calculated. The data were processed by ANOVA. Our results indicated that in comparison to 2009, WI were increased in 2021 in untreated control by 44.2% in MC, 23.6% in MW, 24.6% in MSW and 18.6% in MWS. On the other hand, in treated plots WI have increased only in MC, by 62.5% and lowered by 7.7% in MW, 29.4% in MSW and 60.0% in MWS. WB has been reduced in 2021 significantly on treated as well as untreated plots in all rotation systems. The highest reduction of WB was achieved in untreated control of MWS (42.6%) and treated plot in MC (45.6%). Conclusively, it was proved that even with basic herbicide treatment with soil herbicides crop rotation could be very effec tive in weed control. On the other hand, maize continuous cropping has to be avoided in order to reduce potentials for weed infestation.",
journal = "19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts",
title = "Contribution of long-term crop rotation to weed control in maize",
pages = "215-215"
}
Simić, M., Brankov, M.,& Dragičević, V.. (2022). Contribution of long-term crop rotation to weed control in maize. in 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts, 215-215.
Simić M, Brankov M, Dragičević V. Contribution of long-term crop rotation to weed control in maize. in 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts. 2022;:215-215..
Simić, Milena, Brankov, Milan, Dragičević, Vesna, "Contribution of long-term crop rotation to weed control in maize" in 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts (2022):215-215.

Weed control in sweet maize (Zea mays var. succharata) eco-farming

Simić, Milena; Brankov, Milan; Dragičević, Vesna

(2022)

TY  - CONF
AU  - Simić, Milena
AU  - Brankov, Milan
AU  - Dragičević, Vesna
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/996
AB  - Sustainability in agricultural systems is largely based on increasing biodiversity and reduc ing the input of agrochemicals. Weed species occurrence and distribution in fields is driven by 
anthropogenic factor and cropping measures applied for high yield achievement. In sustainable 
cropping systems with cultivation of specific maize varieties such as sweet maize, special at tention has been usually paid to weed control because these genotypes are additionally sen sitive to herbicides. Sweet maize is mainly consumed directly by humans and its cultivation is 
supposed to be conducted without chemical control of weeds, giving advantage to cover crops. 
Cover crops influence weed infestation level through competition for space, light, water, and 
minerals. The aim of the investigation was to identify the most effective species or mixtures of 
cover crops in weed control of sweet maize in the present agro-ecological conditions. The ex periment was conducted at the Maize Research Institute Zemun Polje, Belgrade, Serbia during 
2014-2016. Sweet maize was grown after different cover crops: V1-common vetch (Vicia sativa 
L.), V2-field pea (Pisum sativum L.), V3-winter oats (Avena sativa L.), V4-fodder kale (Brassica 
oleracea convar. acephala L.), V5-common vetch+oats and V6-field pea+oats, V7- straw and 
V8- bare soil as a control. The preceding crop was winter wheat and each treatment had three 
repetitions. Sowing of cover crops was done in autumn while incorporation by ploughing was 
performed in spring, one week before sweet maize sowing (hybrid ZP SC 421su)in a density of 
65.000 plants ha-1. Six weeks after sowing, the weed association composition was analysed 
by weed species, number of individuals, and biomass determination per m2. After that, weeds 
were removed by hand hoeing and herbicides were not applied. The 19 weed species were 
determined during the investigation while seven species were present in each year and made 
a base of association: Solanum nigrum L., Sorghum halepense (Pers.) L., Chenopodium album
L., C. hybridum L., Amaranthus retroflexus L., A. hybridusL. and A. albus L.. Number of weed 
species, weed individuals and their biomass were significantly affected by year, cover crop, and 
their interaction. The highest number of individuals was detected in 2016 which was favourable 
regarding sum and distribution of precipitation. V1, V2 and V3 were the most efficient cover 
crop treatments in weed control, with the lowest number of weed species and individuals. In 
average for three years, weed biomass was significantly reduced after cultivation of field pea, 
V2 (820.2 g m-2) and its mixture with oats, V6 (794.3 g m-2) in comparison to control, bare soil, 
V8 (1582.6 g m-2).
C3  - 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts
T1  - Weed control in sweet maize (Zea mays var. succharata) eco-farming
SP  - 132
EP  - 132
ER  - 
@conference{
author = "Simić, Milena and Brankov, Milan and Dragičević, Vesna",
year = "2022",
abstract = "Sustainability in agricultural systems is largely based on increasing biodiversity and reduc ing the input of agrochemicals. Weed species occurrence and distribution in fields is driven by 
anthropogenic factor and cropping measures applied for high yield achievement. In sustainable 
cropping systems with cultivation of specific maize varieties such as sweet maize, special at tention has been usually paid to weed control because these genotypes are additionally sen sitive to herbicides. Sweet maize is mainly consumed directly by humans and its cultivation is 
supposed to be conducted without chemical control of weeds, giving advantage to cover crops. 
Cover crops influence weed infestation level through competition for space, light, water, and 
minerals. The aim of the investigation was to identify the most effective species or mixtures of 
cover crops in weed control of sweet maize in the present agro-ecological conditions. The ex periment was conducted at the Maize Research Institute Zemun Polje, Belgrade, Serbia during 
2014-2016. Sweet maize was grown after different cover crops: V1-common vetch (Vicia sativa 
L.), V2-field pea (Pisum sativum L.), V3-winter oats (Avena sativa L.), V4-fodder kale (Brassica 
oleracea convar. acephala L.), V5-common vetch+oats and V6-field pea+oats, V7- straw and 
V8- bare soil as a control. The preceding crop was winter wheat and each treatment had three 
repetitions. Sowing of cover crops was done in autumn while incorporation by ploughing was 
performed in spring, one week before sweet maize sowing (hybrid ZP SC 421su)in a density of 
65.000 plants ha-1. Six weeks after sowing, the weed association composition was analysed 
by weed species, number of individuals, and biomass determination per m2. After that, weeds 
were removed by hand hoeing and herbicides were not applied. The 19 weed species were 
determined during the investigation while seven species were present in each year and made 
a base of association: Solanum nigrum L., Sorghum halepense (Pers.) L., Chenopodium album
L., C. hybridum L., Amaranthus retroflexus L., A. hybridusL. and A. albus L.. Number of weed 
species, weed individuals and their biomass were significantly affected by year, cover crop, and 
their interaction. The highest number of individuals was detected in 2016 which was favourable 
regarding sum and distribution of precipitation. V1, V2 and V3 were the most efficient cover 
crop treatments in weed control, with the lowest number of weed species and individuals. In 
average for three years, weed biomass was significantly reduced after cultivation of field pea, 
V2 (820.2 g m-2) and its mixture with oats, V6 (794.3 g m-2) in comparison to control, bare soil, 
V8 (1582.6 g m-2).",
journal = "19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts",
title = "Weed control in sweet maize (Zea mays var. succharata) eco-farming",
pages = "132-132"
}
Simić, M., Brankov, M.,& Dragičević, V.. (2022). Weed control in sweet maize (Zea mays var. succharata) eco-farming. in 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts, 132-132.
Simić M, Brankov M, Dragičević V. Weed control in sweet maize (Zea mays var. succharata) eco-farming. in 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts. 2022;:132-132..
Simić, Milena, Brankov, Milan, Dragičević, Vesna, "Weed control in sweet maize (Zea mays var. succharata) eco-farming" in 19. EWRS Symposium 2022 “Lighting the future of weed science”, Athens, 20-23.06.2022. - Book of abstracts (2022):132-132.

Status of essential elements in soil and grain of organically produced maize, spelt, and soybean

Dragičević, Vesna; Stoiljkovic, Milovan; Brankov, Milan; Tolimir, Miodrag; Tabaković, Marijenka; Dodevska, Margarita; Simic, Milena

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2022)

TY  - JOUR
AU  - Dragičević, Vesna
AU  - Stoiljkovic, Milovan
AU  - Brankov, Milan
AU  - Tolimir, Miodrag
AU  - Tabaković, Marijenka
AU  - Dodevska, Margarita
AU  - Simic, Milena
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/981
AB  - Organic agriculture offers many benefits through the increased nutritional quality of produced crops, agro-ecosystem preservation, and climate change mitigation. The development of an efficient nutrient management strategy in low-input systems, such as organic agriculture, which supports soil fertility and essential nutrients absorption by crops, is continually exploring. Thus, a study with maize–spelt–soybean rotation during a 5-year period in organic production was established to evaluate the variability in soil organic matter (SOM) and the status of available elements: N, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si from the soil, as well as grain yield (GY) and the content of protein, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si concentration in the grain of spelt, maize, and soybean. Significant variations in mineral elements in the soil, GY, and grain composition were detected. Spelt achieved the highest average GY, while soybean grain was the richest in a majority of examined nutrients. The soil Ca content was important for GY, while the protein level in grain was generally tied to the Mn level in the soil. It was recognized that soil–crop crosstalk is an important strategy for macro- and micro-nutrients management in the soil and grain of organically produced spelt, maize, and soybean. While a reduction in the GY and protein concentration in grain was present over time, it was established that a low-input system under dry-farming conditions supports nutrient availability and accumulation in grain, under semi-arid agro-ecological conditions of central Serbia.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Agriculture
T1  - Status of essential elements in soil and grain of organically produced maize, spelt, and soybean
VL  - 12
IS  - 5
SP  - 702
DO  - https://doi.org/10.3390/agriculture12050702
ER  - 
@article{
author = "Dragičević, Vesna and Stoiljkovic, Milovan and Brankov, Milan and Tolimir, Miodrag and Tabaković, Marijenka and Dodevska, Margarita and Simic, Milena",
year = "2022",
abstract = "Organic agriculture offers many benefits through the increased nutritional quality of produced crops, agro-ecosystem preservation, and climate change mitigation. The development of an efficient nutrient management strategy in low-input systems, such as organic agriculture, which supports soil fertility and essential nutrients absorption by crops, is continually exploring. Thus, a study with maize–spelt–soybean rotation during a 5-year period in organic production was established to evaluate the variability in soil organic matter (SOM) and the status of available elements: N, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si from the soil, as well as grain yield (GY) and the content of protein, P, K, Ca, Mg, Fe, Cu, Mn, Zn, and Si concentration in the grain of spelt, maize, and soybean. Significant variations in mineral elements in the soil, GY, and grain composition were detected. Spelt achieved the highest average GY, while soybean grain was the richest in a majority of examined nutrients. The soil Ca content was important for GY, while the protein level in grain was generally tied to the Mn level in the soil. It was recognized that soil–crop crosstalk is an important strategy for macro- and micro-nutrients management in the soil and grain of organically produced spelt, maize, and soybean. While a reduction in the GY and protein concentration in grain was present over time, it was established that a low-input system under dry-farming conditions supports nutrient availability and accumulation in grain, under semi-arid agro-ecological conditions of central Serbia.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Agriculture",
title = "Status of essential elements in soil and grain of organically produced maize, spelt, and soybean",
volume = "12",
number = "5",
pages = "702",
doi = "https://doi.org/10.3390/agriculture12050702"
}
Dragičević, V., Stoiljkovic, M., Brankov, M., Tolimir, M., Tabaković, M., Dodevska, M.,& Simic, M.. (2022). Status of essential elements in soil and grain of organically produced maize, spelt, and soybean. in Agriculture
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 12(5), 702.
https://doi.org/https://doi.org/10.3390/agriculture12050702
Dragičević V, Stoiljkovic M, Brankov M, Tolimir M, Tabaković M, Dodevska M, Simic M. Status of essential elements in soil and grain of organically produced maize, spelt, and soybean. in Agriculture. 2022;12(5):702.
doi:https://doi.org/10.3390/agriculture12050702 .
Dragičević, Vesna, Stoiljkovic, Milovan, Brankov, Milan, Tolimir, Miodrag, Tabaković, Marijenka, Dodevska, Margarita, Simic, Milena, "Status of essential elements in soil and grain of organically produced maize, spelt, and soybean" in Agriculture, 12, no. 5 (2022):702,
https://doi.org/https://doi.org/10.3390/agriculture12050702 . .

Quality parameterd and potentials of utilization of different maize hybrids for food and feed

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Radosavljević, Milica; Filipović, Milomir; Srdić, Jelena

(Cacak : University of Kragujevac, Faculty of Agronomy, 2021)

TY  - CONF
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Radosavljević, Milica
AU  - Filipović, Milomir
AU  - Srdić, Jelena
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/876
AB  - Quality parameters of six maize hybrids created at the Maize
Research Institute Zemun Polje were investigated in this study. Physical
properties, kernel structure, and chemical composition of one yellow dent
standard and five specialty maize hybrids of different grain color were analyzed.
Whole-grain maize flour is naturally gluten-free which makes it suitable for
persons suffering from celiac disease. Fiber, protein, and oil make maize grain an
essential component for animal feed production. All maize hybrids showed
favorable processing and nutritive characteristics which make them highly suitable
for different uses.
PB  - Cacak : University of Kragujevac, Faculty of Agronomy
C3  - 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova
T1  - Quality parameterd and potentials of utilization of different maize hybrids for food and feed
SP  - 495
EP  - 500
DO  - 10.46793/SBT26.495N
ER  - 
@conference{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Radosavljević, Milica and Filipović, Milomir and Srdić, Jelena",
year = "2021",
abstract = "Quality parameters of six maize hybrids created at the Maize
Research Institute Zemun Polje were investigated in this study. Physical
properties, kernel structure, and chemical composition of one yellow dent
standard and five specialty maize hybrids of different grain color were analyzed.
Whole-grain maize flour is naturally gluten-free which makes it suitable for
persons suffering from celiac disease. Fiber, protein, and oil make maize grain an
essential component for animal feed production. All maize hybrids showed
favorable processing and nutritive characteristics which make them highly suitable
for different uses.",
publisher = "Cacak : University of Kragujevac, Faculty of Agronomy",
journal = "26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova",
title = "Quality parameterd and potentials of utilization of different maize hybrids for food and feed",
pages = "495-500",
doi = "10.46793/SBT26.495N"
}
Nikolić, V., Žilić, S., Simić, M., Radosavljević, M., Filipović, M.,& Srdić, J.. (2021). Quality parameterd and potentials of utilization of different maize hybrids for food and feed. in 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova
Cacak : University of Kragujevac, Faculty of Agronomy., 495-500.
https://doi.org/10.46793/SBT26.495N
Nikolić V, Žilić S, Simić M, Radosavljević M, Filipović M, Srdić J. Quality parameterd and potentials of utilization of different maize hybrids for food and feed. in 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova. 2021;:495-500.
doi:10.46793/SBT26.495N .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Radosavljević, Milica, Filipović, Milomir, Srdić, Jelena, "Quality parameterd and potentials of utilization of different maize hybrids for food and feed" in 26. savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 12-13. mart 2021. godine - Zbornik radova (2021):495-500,
https://doi.org/10.46793/SBT26.495N . .

Possibilities of dried distillers' grains with solubles application in animal feed

Nikolić, Valentina; Milašinović Šeremešić, Marija; Radosavljević, Milica; Simić, Marijana; Žilić, Slađana; Vančetović, Jelena; Božinović, Sofija

(Novi Sad : National Society of Processing and Energy in Agriculture, 2021)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Milašinović Šeremešić, Marija
AU  - Radosavljević, Milica
AU  - Simić, Marijana
AU  - Žilić, Slađana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/845
AB  - The possibility of utilization of dried distillers’ grains with solubles (DDGS) in animal feed was investigated. Samples of maize
hybrids ZP 548 and ZP 655b, and DDGS obtained from hybrids ZP 548 and ZP 548c were used as components of the feed. The total
protein content ranged from 12.42% to 31.18%, moisture from 5.49% to 9.55, and ash content from 1.85% to 4.37%. The contents of
NDF, ADF, ADL, hemicellulose and cellulose fibers ranged from: 13.90% to 48.13%; 2.9% to 20.69%; 0.27% to 2.44%; 10.69% to
30.17%, 2.62% to 18.32%, respectively. In vitro, dry matter digestibility ranged from 55.20% to 89.76%. It was concluded that the
samples of DDG obtained from red and yellow maize hybrids kernel are very suitable as components for the preparation of animal
feed.
AB  - Ispitivana je mogućnost primene suve kukuruzne džibre u smešama za ishranu domaćih životinja. Kao komponente korišćeni su
uzorci cele biljke i zrna hibrida ZP 548 i ZP 655b i uzorci džibre dobijeni od hibrida ZP 548 i ZP 548c (hibrid crvenog zrna). Uzorci
kukuruzne džibre, sporednog produkta iz proizvodnje bioetanola, dobijeni su postupkom odvojene dvostepene hidrolize i fermentacije
skroba iz kukuruznog brašna. Metoda se zasniva na korišćenju enzimskih preparata u fazama hidrolize i primeni kvasca S. cerevisiae
var. ellipsoideus tokom fermentacije. Uzorci džibre sušeni su u ventilacionoj sušnici na temperaturi 60˚C u trajanju od 48h, a potom
samleveni u laboratorijskom mlinu. Sadržaj proteina kretao se od 12,42% do 31,18%, vlage od 5,49% do 9,55%, a pepela od 1,85%
do 4,37%. Sadržaj NDF-a kretao se od 13,90% (zrno ZP 548 + 15% džibre ZP 548) do 48,13% (cela biljka ZP 655b + 20% džibre
ZP 548c); ADF-a od 2,96% (zrno ZP 548 +15% džibre ZP 548c) do 20,69% (cela biljka ZP 655b + 10% džibre ZP 548c); ADL-a od
0,27% do 2,44f%: hemiceluloze od 10,69% do 30,17% i celuloze od 2,62% do 18,32%. In vitro svarljivost suve materije određena
enzimskom pepsin-celulaznom metodom kretala se u rasponu od 55,20% do 89,76%. Na osnovu dobijenih rezultata ustanovljeno je
da su uzorci suve kukuruzne džibre dobijeni od zrna žutog i crvenog hibrida kukuruza pogodni kao hranivo za pripremu smeša za
ishranu domaćih životinja.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Possibilities of dried distillers' grains with solubles application in animal feed
T1  - Mogućnosti primene suve destilerijske džibre u smešama za ishranu životinja
VL  - 25
IS  - 3
SP  - 96
EP  - 100
DO  - 10.5937/jpea25-34890
ER  - 
@article{
author = "Nikolić, Valentina and Milašinović Šeremešić, Marija and Radosavljević, Milica and Simić, Marijana and Žilić, Slađana and Vančetović, Jelena and Božinović, Sofija",
year = "2021",
abstract = "The possibility of utilization of dried distillers’ grains with solubles (DDGS) in animal feed was investigated. Samples of maize
hybrids ZP 548 and ZP 655b, and DDGS obtained from hybrids ZP 548 and ZP 548c were used as components of the feed. The total
protein content ranged from 12.42% to 31.18%, moisture from 5.49% to 9.55, and ash content from 1.85% to 4.37%. The contents of
NDF, ADF, ADL, hemicellulose and cellulose fibers ranged from: 13.90% to 48.13%; 2.9% to 20.69%; 0.27% to 2.44%; 10.69% to
30.17%, 2.62% to 18.32%, respectively. In vitro, dry matter digestibility ranged from 55.20% to 89.76%. It was concluded that the
samples of DDG obtained from red and yellow maize hybrids kernel are very suitable as components for the preparation of animal
feed., Ispitivana je mogućnost primene suve kukuruzne džibre u smešama za ishranu domaćih životinja. Kao komponente korišćeni su
uzorci cele biljke i zrna hibrida ZP 548 i ZP 655b i uzorci džibre dobijeni od hibrida ZP 548 i ZP 548c (hibrid crvenog zrna). Uzorci
kukuruzne džibre, sporednog produkta iz proizvodnje bioetanola, dobijeni su postupkom odvojene dvostepene hidrolize i fermentacije
skroba iz kukuruznog brašna. Metoda se zasniva na korišćenju enzimskih preparata u fazama hidrolize i primeni kvasca S. cerevisiae
var. ellipsoideus tokom fermentacije. Uzorci džibre sušeni su u ventilacionoj sušnici na temperaturi 60˚C u trajanju od 48h, a potom
samleveni u laboratorijskom mlinu. Sadržaj proteina kretao se od 12,42% do 31,18%, vlage od 5,49% do 9,55%, a pepela od 1,85%
do 4,37%. Sadržaj NDF-a kretao se od 13,90% (zrno ZP 548 + 15% džibre ZP 548) do 48,13% (cela biljka ZP 655b + 20% džibre
ZP 548c); ADF-a od 2,96% (zrno ZP 548 +15% džibre ZP 548c) do 20,69% (cela biljka ZP 655b + 10% džibre ZP 548c); ADL-a od
0,27% do 2,44f%: hemiceluloze od 10,69% do 30,17% i celuloze od 2,62% do 18,32%. In vitro svarljivost suve materije određena
enzimskom pepsin-celulaznom metodom kretala se u rasponu od 55,20% do 89,76%. Na osnovu dobijenih rezultata ustanovljeno je
da su uzorci suve kukuruzne džibre dobijeni od zrna žutog i crvenog hibrida kukuruza pogodni kao hranivo za pripremu smeša za
ishranu domaćih životinja.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Possibilities of dried distillers' grains with solubles application in animal feed, Mogućnosti primene suve destilerijske džibre u smešama za ishranu životinja",
volume = "25",
number = "3",
pages = "96-100",
doi = "10.5937/jpea25-34890"
}
Nikolić, V., Milašinović Šeremešić, M., Radosavljević, M., Simić, M., Žilić, S., Vančetović, J.,& Božinović, S.. (2021). Possibilities of dried distillers' grains with solubles application in animal feed. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 25(3), 96-100.
https://doi.org/10.5937/jpea25-34890
Nikolić V, Milašinović Šeremešić M, Radosavljević M, Simić M, Žilić S, Vančetović J, Božinović S. Possibilities of dried distillers' grains with solubles application in animal feed. in Journal on Processing and Energy in Agriculture. 2021;25(3):96-100.
doi:10.5937/jpea25-34890 .
Nikolić, Valentina, Milašinović Šeremešić, Marija, Radosavljević, Milica, Simić, Marijana, Žilić, Slađana, Vančetović, Jelena, Božinović, Sofija, "Possibilities of dried distillers' grains with solubles application in animal feed" in Journal on Processing and Energy in Agriculture, 25, no. 3 (2021):96-100,
https://doi.org/10.5937/jpea25-34890 . .

The role of maize hybrids in current trends of bioethanol production

Nikolić, Valentina; Žilić, Slađana; Radosavljević, Milica; Simić, Marijana

(Belgrade : Serbian Association of Plant Breedings and Seed Producers, 2021)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Radosavljević, Milica
AU  - Simić, Marijana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/852
AB  - Bioethanol is a biofuel that is mostly used as a replacement for fossil fuels worldwide with
yearly production reaching nearly 110 billion liters in 2019. Trends of producing this alternative
fuel are rising and maize is considered as one of the best renewable raw materials for the production of fuel ethanol due to the high content of starch in the grain. Taking into account that
Serbia is one of the most prominent maize producers in Europe, the surpluses of this crop could
be directed toward bioethanol production. Even though there is no organized production and
consumption of bioethanol as an automotive fuel in Serbia, the Serbian Government has recently
introduced some new regulations regarding biofuels. However, due to the reduction of economic
activities since the onset of the COVID-19 pandemic in 2020, the global demand for crude oil has
fallen sharply, negatively affecting the gasoline demand, and thus bioethanol, which makes the
future of this alternative fuel production notably uncertain.
AB  - Bioetanol je biogorivo koje se uglavnom koristi kao zamena za fosilna goriva širom sveta,
a godišnja proizvodnja dostigla je gotovo 110 milijardi litara u 2019. godini. Trendovi proizvodnje ovog alternativnog goriva rastu, a kukuruz se smatra jednom od najboljih obnovljivih
sirovina za proizvodnju bioetanola zahvaljujući visokom sadržaju skroba u zrnu. Uzimajući u
obzir da je Srbija jedan od najistaknutijih proizvođača kukuruza u Evropi, viškovi useva ove
poljoprivredne kulture mogli bi se usmeriti ka proizvodnji bioetanola. Iako u Srbiji ne postoji
organizovana proizvodnja i potrošnja bioetanola kao transportnog goriva, Vlada Republike Srbije nedavno je usvojila nekoliko novih uredbi u vezi sa stavljanjem u promet, obeležavanjem i
potrošnjom biogoriva. Međutim, zbog smanjenja ekonomskih aktivnosti od početka pandemije
virusa COVID-19 tokom 2020. godine, globalna potražnja za sirovom naftom naglo je opala, što
je negativno uticalo na potražnju za benzinom, a time i za bioetanolom, zbog čega je budućnost
proizvodnje ovog alternativnog goriva za sada u velikoj meri neizvesna.
PB  - Belgrade : Serbian Association of Plant Breedings and Seed Producers
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - The role of maize hybrids in current trends of bioethanol production
VL  - 26
IS  - 2
SP  - 21
EP  - 29
DO  - 10.5937/SelSem2002021N
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Radosavljević, Milica and Simić, Marijana",
year = "2021",
abstract = "Bioethanol is a biofuel that is mostly used as a replacement for fossil fuels worldwide with
yearly production reaching nearly 110 billion liters in 2019. Trends of producing this alternative
fuel are rising and maize is considered as one of the best renewable raw materials for the production of fuel ethanol due to the high content of starch in the grain. Taking into account that
Serbia is one of the most prominent maize producers in Europe, the surpluses of this crop could
be directed toward bioethanol production. Even though there is no organized production and
consumption of bioethanol as an automotive fuel in Serbia, the Serbian Government has recently
introduced some new regulations regarding biofuels. However, due to the reduction of economic
activities since the onset of the COVID-19 pandemic in 2020, the global demand for crude oil has
fallen sharply, negatively affecting the gasoline demand, and thus bioethanol, which makes the
future of this alternative fuel production notably uncertain., Bioetanol je biogorivo koje se uglavnom koristi kao zamena za fosilna goriva širom sveta,
a godišnja proizvodnja dostigla je gotovo 110 milijardi litara u 2019. godini. Trendovi proizvodnje ovog alternativnog goriva rastu, a kukuruz se smatra jednom od najboljih obnovljivih
sirovina za proizvodnju bioetanola zahvaljujući visokom sadržaju skroba u zrnu. Uzimajući u
obzir da je Srbija jedan od najistaknutijih proizvođača kukuruza u Evropi, viškovi useva ove
poljoprivredne kulture mogli bi se usmeriti ka proizvodnji bioetanola. Iako u Srbiji ne postoji
organizovana proizvodnja i potrošnja bioetanola kao transportnog goriva, Vlada Republike Srbije nedavno je usvojila nekoliko novih uredbi u vezi sa stavljanjem u promet, obeležavanjem i
potrošnjom biogoriva. Međutim, zbog smanjenja ekonomskih aktivnosti od početka pandemije
virusa COVID-19 tokom 2020. godine, globalna potražnja za sirovom naftom naglo je opala, što
je negativno uticalo na potražnju za benzinom, a time i za bioetanolom, zbog čega je budućnost
proizvodnje ovog alternativnog goriva za sada u velikoj meri neizvesna.",
publisher = "Belgrade : Serbian Association of Plant Breedings and Seed Producers, Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "The role of maize hybrids in current trends of bioethanol production",
volume = "26",
number = "2",
pages = "21-29",
doi = "10.5937/SelSem2002021N"
}
Nikolić, V., Žilić, S., Radosavljević, M.,& Simić, M.. (2021). The role of maize hybrids in current trends of bioethanol production. in Selekcija i semenarstvo
Belgrade : Serbian Association of Plant Breedings and Seed Producers., 26(2), 21-29.
https://doi.org/10.5937/SelSem2002021N
Nikolić V, Žilić S, Radosavljević M, Simić M. The role of maize hybrids in current trends of bioethanol production. in Selekcija i semenarstvo. 2021;26(2):21-29.
doi:10.5937/SelSem2002021N .
Nikolić, Valentina, Žilić, Slađana, Radosavljević, Milica, Simić, Marijana, "The role of maize hybrids in current trends of bioethanol production" in Selekcija i semenarstvo, 26, no. 2 (2021):21-29,
https://doi.org/10.5937/SelSem2002021N . .
2

Application of standard germination and vigour tests for seed quality assessment of maize inbred lines

Milivojević, Marija; Srdić, Jelena; Filipović, Milomir; Petrović, Tanja; Branković-Radojčić, Dragana V.; Marković, Ksenija; Boćanski, Jan

(Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije, 2021)

TY  - JOUR
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Filipović, Milomir
AU  - Petrović, Tanja
AU  - Branković-Radojčić, Dragana V.
AU  - Marković, Ksenija
AU  - Boćanski, Jan
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/924
AB  - Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field 
conditions and seed longevity is essential for seed companies. The objective of this work was to 
evaluate different vigour tests for detecting differences among naturally aged seeds and to assess 
the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed 
of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different 
heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings 
was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines 
was detected in seed vigour determined by three tests, in particular by the accelerated ageing test.
Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines 
with Lancaster background. The correlation between the cold test and the accelerated ageing test 
was significant, while the radical emergence test had a poor correlation with them. Obtained 
results indicate that the application of either cold test or accelerated ageing test is reliable for 
screening seed vigour of maize inbred lines.
AB  - Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod-
nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog
rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog
semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena
i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza
selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim
grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom
standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad-
nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana
Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve
parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu
vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad-
nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom
nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa
ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa
njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana
za skrining vigora semena samooplodnih linija kukuruza
PB  - Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Application of standard germination and vigour tests for seed quality assessment of maize inbred lines
T1  - Primena testova standardne klijavosti i vigor testova u proceni kvaliteta semena samooplodnih linija kukuruza
VL  - 27
IS  - 2
SP  - 35
EP  - 45
DO  - 10.5937/SelSem2102035M
ER  - 
@article{
author = "Milivojević, Marija and Srdić, Jelena and Filipović, Milomir and Petrović, Tanja and Branković-Radojčić, Dragana V. and Marković, Ksenija and Boćanski, Jan",
year = "2021",
abstract = "Information regarding the adaptation of maize (Zea mays L.) inbred lines to various field 
conditions and seed longevity is essential for seed companies. The objective of this work was to 
evaluate different vigour tests for detecting differences among naturally aged seeds and to assess 
the relationship between seed quality parameters and pedigree data of maize inbred lines. A seed 
of 15 maize inbred lines, selected at Maize Research Institute Zemun Polje, belonging to different 
heterotic groups was produced during 2011. Seed quality was assessed in 2015 by standard ger mination, radical emergence, accelerated ageing, and cold test. Root length of 4-day old seedlings 
was measured in all applied tests. Analysis of variance showed that genotype and storage condi tions had a significant effect on all seed quality parameters. Large variation among inbred lines 
was detected in seed vigour determined by three tests, in particular by the accelerated ageing test.
Cold test results showed very good chilling tolerance of tested genotypes, except two inbred lines 
with Lancaster background. The correlation between the cold test and the accelerated ageing test 
was significant, while the radical emergence test had a poor correlation with them. Obtained 
results indicate that the application of either cold test or accelerated ageing test is reliable for 
screening seed vigour of maize inbred lines., Semenskim kompanijama su od izuzetne važnosti informacije o adaptibilnosti samooplod-
nih linija kukuruza (Zea mays L ) različitim uslovima u polju i dugovečnosti semena Cilj ovog
rada bio je da se izvrši procena vigor testova za otkrivanje razlika između prirodno ostarelog
semena u različitim uslovima čuvanja i da se proceni odnos između parametara kvaliteta semena
i podataka o pedigreu samooplodnih linija kukuruza Seme 15 samooplodnih linija kukuruza
selekcionisanih u “Institutu za kukuruz” - Zemun Polje, koje pripadaju različitim heterotičnim
grupama, proizvedeno je 2011 godine Kvalitet semena je ocenjivan 2015 godine primenom
standardnog testa za klijavost, testa za pojavu primarnog korena, ubrzanim starenjem i hlad-
nim testom U svim primenjenim ispitivanjima merena je dužina korena klijanaca starih 4 dana
Analiza varijanse je pokazala da genotip i uslovi čuvanja semena imaju značajan uticaj na sve
parametre kvaliteta semena Samooplodne linije kukuruza su se značajno razlikovale u pogledu
vigora semena na osnovu tri primenjena testa, a naročito testa ubrzanog starenja Rezultati hlad-
nog testa su pokazali visoku tolerantnost ispitivanih genotipova na niske temperature prilikom
nicanja, osim dve samooplodne linije Lancaster porekla Korelacija između hladnog testa i testa
ubrzanog starenja bila je značajna, dok je test pojave primarnog korena imao slabu korelaciju sa
njima Dobijeni rezultati ukazuju da je primena hladnog testa i testa ubrzanog starenja pouzdana
za skrining vigora semena samooplodnih linija kukuruza",
publisher = "Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Application of standard germination and vigour tests for seed quality assessment of maize inbred lines, Primena testova standardne klijavosti i vigor testova u proceni kvaliteta semena samooplodnih linija kukuruza",
volume = "27",
number = "2",
pages = "35-45",
doi = "10.5937/SelSem2102035M"
}
Milivojević, M., Srdić, J., Filipović, M., Petrović, T., Branković-Radojčić, D. V., Marković, K.,& Boćanski, J.. (2021). Application of standard germination and vigour tests for seed quality assessment of maize inbred lines. in Selekcija i semenarstvo
Belgrade-Zemun : Društvo selekcionera i semenara Republike Srbije., 27(2), 35-45.
https://doi.org/10.5937/SelSem2102035M
Milivojević M, Srdić J, Filipović M, Petrović T, Branković-Radojčić DV, Marković K, Boćanski J. Application of standard germination and vigour tests for seed quality assessment of maize inbred lines. in Selekcija i semenarstvo. 2021;27(2):35-45.
doi:10.5937/SelSem2102035M .
Milivojević, Marija, Srdić, Jelena, Filipović, Milomir, Petrović, Tanja, Branković-Radojčić, Dragana V., Marković, Ksenija, Boćanski, Jan, "Application of standard germination and vigour tests for seed quality assessment of maize inbred lines" in Selekcija i semenarstvo, 27, no. 2 (2021):35-45,
https://doi.org/10.5937/SelSem2102035M . .

Genetic distance of maize inbreds for prediction of heterosis and combining ability

Perić, Sanja; Stevanović, Milan; Prodanović, Slaven; Mladenović-Drinić, Snežana; Grčić, Nikola; Kandić, Vesna; Pavlov, Jovan

(Belgrade : Serbian Genetics Society, 2021)

TY  - JOUR
AU  - Perić, Sanja
AU  - Stevanović, Milan
AU  - Prodanović, Slaven
AU  - Mladenović-Drinić, Snežana
AU  - Grčić, Nikola
AU  - Kandić, Vesna
AU  - Pavlov, Jovan
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/834
AB  - A panel of seven maize inbred lines belonging to Zemun Polje commercial pool were
genotypized using SNPs bead chip. 21 hybrids, developed according to the half dialel
mating design, were tested in the field together with inbred lines per se. The goal of the
study was to determine the genetic distance among seven maize inbred lines and to
establish whether there was a significant correlation between the genetic distance among
parental inbreds and grain yield, specific combining abilities (SCA) and high-parent (HP)
heterosis for the grain yield. The inbred lines ZPL2 and ZPL4 with the genetic distance of
0.487 were the most genetically distant parents, while inbred lines ZPL1 and ZPL2 with
the genetic distance of 0.191 were the closest ones. Three subclusters of inbred lines were
distinguished in the dendrogram. Inbred lines ZPL5, ZPL6, ZPL3 and ZPL7 were
grouped into the first subcluster, while inbred lines ZPL1 and ZPL2, i.e. the inbred line
ZPL4 were grouped in the second, i.e. the third subcluster, respectively. The values of the
Spearman's rank correlation coefficient between the genetic distance among inbred lines
based on the SNP markers, and grain yield, specific combining abilities (SCA) and high-
parent (HP) heterosis were positive and statistically significant. The highest correlation
coefficient was exhibited between the grain yield and high-parent (HP) heterosis (0.93),
and then between the genetic distance and the grain yield (0.92) as well as between the
genetic distance and high-parent (HP) heterosis (0.91).
AB  - Panel od sedam inbred linija kukuruza koje pripadaju komercijalnom pulu Instituta za kukuruz u
Zemun Polju je analiziran pomoću SNP čipa. Hibridi dobijeni po metodu nepotpunog dialela su
ispitivani u polju zajedno sa inbred linijama per se. Cilj ovog istraživanja je bio da se utvrdi
genetička distanca između inbred linija kukuruza i da se ispita da li postoji značajna korelacija
između genetičke distance roditeljskih linija, posebnih kombinacionih sposobnosti (PKS) i
heterozisa u odnosu na boljeg roditelja (HPH) za osobinu prinos zrna. Genetički najudaljenije
linije su bile ZPL2 i ZPL4 sa genetičkom distancom 0.487, dok su najbliže bile inbred linije
ZPL1 i ZPL2 sa genetičkom distancom 0.191, kao i inbred linije ZPL5 i ZPL6 sa genetičkom
distancom 0.196. Dendrogram je podelio inbred linije u tri različita subklastera. Prvom
subklasteru pripadaju inbred linije ZPL5, ZPL6, ZPL3 i ZPL7, drugom inbred linije ZPL1 i
ZPL2 i trećem inbred linija ZPL4. Vrednosti Sperman-ovog koeficijenta korelacije ranga između
genetičke distance zasnovane na SNP markerima, prinosa zrna, posebnih kombinacionih
sposobnosti (PKS) i heterozisa u odnosu na boljeg roditelja (HPH) su bile pozitivne i statistički
značajne. Najviši koeficijent korelacije je bio između prinosa zrna i HPH (0,93), zatim između
genetičke distance i prinosa (0.92) i između genetičke distance i HPH (0.91).
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Genetic distance of maize inbreds for prediction of heterosis and combining ability
VL  - 53
IS  - 3
SP  - 1219
EP  - 1228
DO  - 10.2298/GENSR2103219P
ER  - 
@article{
author = "Perić, Sanja and Stevanović, Milan and Prodanović, Slaven and Mladenović-Drinić, Snežana and Grčić, Nikola and Kandić, Vesna and Pavlov, Jovan",
year = "2021",
abstract = "A panel of seven maize inbred lines belonging to Zemun Polje commercial pool were
genotypized using SNPs bead chip. 21 hybrids, developed according to the half dialel
mating design, were tested in the field together with inbred lines per se. The goal of the
study was to determine the genetic distance among seven maize inbred lines and to
establish whether there was a significant correlation between the genetic distance among
parental inbreds and grain yield, specific combining abilities (SCA) and high-parent (HP)
heterosis for the grain yield. The inbred lines ZPL2 and ZPL4 with the genetic distance of
0.487 were the most genetically distant parents, while inbred lines ZPL1 and ZPL2 with
the genetic distance of 0.191 were the closest ones. Three subclusters of inbred lines were
distinguished in the dendrogram. Inbred lines ZPL5, ZPL6, ZPL3 and ZPL7 were
grouped into the first subcluster, while inbred lines ZPL1 and ZPL2, i.e. the inbred line
ZPL4 were grouped in the second, i.e. the third subcluster, respectively. The values of the
Spearman's rank correlation coefficient between the genetic distance among inbred lines
based on the SNP markers, and grain yield, specific combining abilities (SCA) and high-
parent (HP) heterosis were positive and statistically significant. The highest correlation
coefficient was exhibited between the grain yield and high-parent (HP) heterosis (0.93),
and then between the genetic distance and the grain yield (0.92) as well as between the
genetic distance and high-parent (HP) heterosis (0.91)., Panel od sedam inbred linija kukuruza koje pripadaju komercijalnom pulu Instituta za kukuruz u
Zemun Polju je analiziran pomoću SNP čipa. Hibridi dobijeni po metodu nepotpunog dialela su
ispitivani u polju zajedno sa inbred linijama per se. Cilj ovog istraživanja je bio da se utvrdi
genetička distanca između inbred linija kukuruza i da se ispita da li postoji značajna korelacija
između genetičke distance roditeljskih linija, posebnih kombinacionih sposobnosti (PKS) i
heterozisa u odnosu na boljeg roditelja (HPH) za osobinu prinos zrna. Genetički najudaljenije
linije su bile ZPL2 i ZPL4 sa genetičkom distancom 0.487, dok su najbliže bile inbred linije
ZPL1 i ZPL2 sa genetičkom distancom 0.191, kao i inbred linije ZPL5 i ZPL6 sa genetičkom
distancom 0.196. Dendrogram je podelio inbred linije u tri različita subklastera. Prvom
subklasteru pripadaju inbred linije ZPL5, ZPL6, ZPL3 i ZPL7, drugom inbred linije ZPL1 i
ZPL2 i trećem inbred linija ZPL4. Vrednosti Sperman-ovog koeficijenta korelacije ranga između
genetičke distance zasnovane na SNP markerima, prinosa zrna, posebnih kombinacionih
sposobnosti (PKS) i heterozisa u odnosu na boljeg roditelja (HPH) su bile pozitivne i statistički
značajne. Najviši koeficijent korelacije je bio između prinosa zrna i HPH (0,93), zatim između
genetičke distance i prinosa (0.92) i između genetičke distance i HPH (0.91).",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Genetic distance of maize inbreds for prediction of heterosis and combining ability",
volume = "53",
number = "3",
pages = "1219-1228",
doi = "10.2298/GENSR2103219P"
}
Perić, S., Stevanović, M., Prodanović, S., Mladenović-Drinić, S., Grčić, N., Kandić, V.,& Pavlov, J.. (2021). Genetic distance of maize inbreds for prediction of heterosis and combining ability. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1219-1228.
https://doi.org/10.2298/GENSR2103219P
Perić S, Stevanović M, Prodanović S, Mladenović-Drinić S, Grčić N, Kandić V, Pavlov J. Genetic distance of maize inbreds for prediction of heterosis and combining ability. in Genetika. 2021;53(3):1219-1228.
doi:10.2298/GENSR2103219P .
Perić, Sanja, Stevanović, Milan, Prodanović, Slaven, Mladenović-Drinić, Snežana, Grčić, Nikola, Kandić, Vesna, Pavlov, Jovan, "Genetic distance of maize inbreds for prediction of heterosis and combining ability" in Genetika, 53, no. 3 (2021):1219-1228,
https://doi.org/10.2298/GENSR2103219P . .
1

Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia

Nikolić, Milica; Savić, Iva; Nikolić, Ana; Jauković, Marko; Kandić, Vesna; Stevanović, Milan; Stanković, Slavica

(Basel : Multidisciplinary Digital Publishing Institute (MDPI), 2021)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Nikolić, Ana
AU  - Jauković, Marko
AU  - Kandić, Vesna
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/836
AB  - In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.
PB  - Basel : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Toxins
T1  - Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia
VL  - 13
SP  - 847
DO  - 10.3390/toxins13120847
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Nikolić, Ana and Jauković, Marko and Kandić, Vesna and Stevanović, Milan and Stanković, Slavica",
year = "2021",
abstract = "In Serbia, aspergillus ear rot caused by the disease pathogen Aspergillus parasiticus
(A. parasiticus) was first detected in 2012 under both field and storage conditions. Global climate
shifts, primarily warming, favour the contamination of maize with aflatoxins in temperate climates,
including Serbia. A five-year study (2012–2016) comprising of 46 A. parasiticus strains isolated from
maize kernels was performed to observe the morphological, molecular, pathogenic, and toxigenic
traits of this pathogen. The HPLC method was applied to evaluate mycotoxin concentrations in this
causal agent. The A. parasiticus isolates synthesised mainly aflatoxin AFB1 (84.78%). The percentage
of isolates synthesising aflatoxin AFG1 (15.22%) was considerably lower. Furthermore, the concen-
tration of AFG1 was higher than that of AFB1 in eight isolates. The polyphase approach, used to
characterise isolates, showed that they were A. parasiticus species. This identification was verified by
the multiplex RLFP-PCR detection method with the use of restriction enzymes. These results form an
excellent baseline for further studies with the aim of application in the production, processing, and
storage of cereal grains and seeds, and in technological processes to ensure the safe production of
food and feed.",
publisher = "Basel : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Toxins",
title = "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia",
volume = "13",
pages = "847",
doi = "10.3390/toxins13120847"
}
Nikolić, M., Savić, I., Nikolić, A., Jauković, M., Kandić, V., Stevanović, M.,& Stanković, S.. (2021). Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins
Basel : Multidisciplinary Digital Publishing Institute (MDPI)., 13, 847.
https://doi.org/10.3390/toxins13120847
Nikolić M, Savić I, Nikolić A, Jauković M, Kandić V, Stevanović M, Stanković S. Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia. in Toxins. 2021;13:847.
doi:10.3390/toxins13120847 .
Nikolić, Milica, Savić, Iva, Nikolić, Ana, Jauković, Marko, Kandić, Vesna, Stevanović, Milan, Stanković, Slavica, "Toxigenic species Aspergillus parasiticus originating from Maize Kernels grown in Serbia" in Toxins, 13 (2021):847,
https://doi.org/10.3390/toxins13120847 . .
1

The occurrence of mycotoxins in sweet maize hybrids

Nikolić, Milica; Srdić, Jelena; Savić, Iva; Žilić, Slađana; Stevanović, Milan; Kandić, Vesna; Stanković, Slavica

(Belgrade : Serbian Genetics Society, 2021)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Srdić, Jelena
AU  - Savić, Iva
AU  - Žilić, Slađana
AU  - Stevanović, Milan
AU  - Kandić, Vesna
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/833
AB  - The objective of the present study was to investigate the susceptibility of maize hybrids to the
natural mycotoxins contamination: aflatoxin total (AFLA), deoxynivalenol (DON),
zearalenon (ZEA) and fumonisins (FB). Hybrids were grown during two production years
(2019 and 2020) in two locations. Mycotoxin contamination of maize grains was evaluated in
five sweet maize hybrids. Contamination level of investigated hybrids of first cluster was
mainly by below average values of ZEA (all equal to zero) and DON. Hybrids PK4 (S) 2020,
PK6 (MS) 2020 and PK4 (MS) 2020 had below average values for AFLA, while remaining
treatments of this cluster have mostly elevated values of this mycotoxin. Contamination level
of investigated hybrids of second cluster mostly had increased values of mycotoxins ZEA
and DON and below average values of AFLA. Samples did not contain fumonisins.
Mycotoxin contamination were significantly affected by hybrids and years. We have
established that DON and ZEA levels were influenced by the environmental conditions.
There were no significant effects of location on the level of AFLA in the sweet maize
hybrids. The variation in the properties of mycotoxin content (DON) was significantly
influenced by hybrids, and there was no significance of hybrids x location interaction.
Differences were more expressed for the content of ZEA and AFLA compared to the content
of DON. Hybrid PK1 had the lowest content of DON, while it had the highest content of
ZEA. Mycotoxin analyses showed that in all tested hybrids, levels of AFLA, DON, ZEA and
FBs were below the maximum permissible levels stipulated by the legislation of the
European Union and the Republic of Serbia in maize intended for direct human consumption.
These results confirmed that the susceptibility of hybrids is one of the important risks, in addition to climatic factors, for the appearance of toxigenic fungi and their mycotoxins.
Genotype tolerance is very important as a preventive measure, which indicates that breeders
have to pay attention to it in sweet maize breeding programs.
AB  - Cilj ovog rada bio je da se prouči osteljivost hibrida kukuruza na prirodnu kontaminaciju
mikotoksinima (aflatoksin B1 (AFB1), deoksnivalenol (DON), zearalenol (ZEA) i fumonizini
(FB)). Hibrdi su gajeni tokom dve proizvodne godine (2019. i 2020) u dve lokacije.
Kontaminacija zrna kukuruza mikotoksinima ispitivana je na pet hibrida kukuruza šećerca. Nivo
kontaminacije proučavanih hibrida prvog klastera bio je uglavnom ispod prosečnih vrednosti
ZEA (sve vrednosti su bile nula) i DON. Vrednosti AFLA kod hibrida PK4 (S) 2020, PK6 (MS)
2020 i PK4 (MS) 2020 su bile ispod prosečne vrednosti, dok su vrednosti ovog mikotoksina bile
više od prosečnih vrednosti za ostale tretmane ovog klastera. Nivoi kontaminacije proučavanih
hibrida drugog klastera su uglavnom bili viši za mikotoksine ZEA i DON i niži za AFLA.
Fumnonizni nisu utrvđreni u uzorcima. Na kontaminaciju mikotoksinima značajno su uticali
hibiridi i godine. Utvrđeno je da su uslovi sredine uticali na nivoe DON i ZEA. Lokacija nije
značajno uticala na nivo AFLA kod hibrida kukuruza šećerca. Hibridi su značajno uticali na
variranje sadržaja mikotoksina (DON), dok hibrid × lokacija interakcija nije bila značajna.
Razlike su bile izraženije za sadržaj ZEA i ALFA nego za sadržaj DON. Najniži sadržaj DON
utvrđen je kod hibrida PK1, kod koga je sadržaj ZEA bio najviši. Analize mikotoksina pokazuju
da su nivoi AFLA, DON, ZEA i FB u svim ispitivanim hibridima bili ispod maksimalno
dozvoljenih nivoa koji su propisani zankonima Evropske Unije i Republike Srbije za kukuruz
koji je namenjen za direktnu ljudsku konzumaciju. Ovi rezultati potvrđuju da je osetljivost
hibrida jedan od važnih rizika pored klimatskih faktora za pojavu toksigenih gljiva i njihovih
mikotoksima. Tolerantnost genotipa je veoma važna preventivna mera, na koju oplemenjivači
moraju da obrate pažnju u programima oplemenjivanja kukuruza šećerca.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - The occurrence of mycotoxins in sweet maize hybrids
VL  - 53
IS  - 3
SP  - 1311
EP  - 1320
DO  - 10.2298/GENSR2103311N
ER  - 
@article{
author = "Nikolić, Milica and Srdić, Jelena and Savić, Iva and Žilić, Slađana and Stevanović, Milan and Kandić, Vesna and Stanković, Slavica",
year = "2021",
abstract = "The objective of the present study was to investigate the susceptibility of maize hybrids to the
natural mycotoxins contamination: aflatoxin total (AFLA), deoxynivalenol (DON),
zearalenon (ZEA) and fumonisins (FB). Hybrids were grown during two production years
(2019 and 2020) in two locations. Mycotoxin contamination of maize grains was evaluated in
five sweet maize hybrids. Contamination level of investigated hybrids of first cluster was
mainly by below average values of ZEA (all equal to zero) and DON. Hybrids PK4 (S) 2020,
PK6 (MS) 2020 and PK4 (MS) 2020 had below average values for AFLA, while remaining
treatments of this cluster have mostly elevated values of this mycotoxin. Contamination level
of investigated hybrids of second cluster mostly had increased values of mycotoxins ZEA
and DON and below average values of AFLA. Samples did not contain fumonisins.
Mycotoxin contamination were significantly affected by hybrids and years. We have
established that DON and ZEA levels were influenced by the environmental conditions.
There were no significant effects of location on the level of AFLA in the sweet maize
hybrids. The variation in the properties of mycotoxin content (DON) was significantly
influenced by hybrids, and there was no significance of hybrids x location interaction.
Differences were more expressed for the content of ZEA and AFLA compared to the content
of DON. Hybrid PK1 had the lowest content of DON, while it had the highest content of
ZEA. Mycotoxin analyses showed that in all tested hybrids, levels of AFLA, DON, ZEA and
FBs were below the maximum permissible levels stipulated by the legislation of the
European Union and the Republic of Serbia in maize intended for direct human consumption.
These results confirmed that the susceptibility of hybrids is one of the important risks, in addition to climatic factors, for the appearance of toxigenic fungi and their mycotoxins.
Genotype tolerance is very important as a preventive measure, which indicates that breeders
have to pay attention to it in sweet maize breeding programs., Cilj ovog rada bio je da se prouči osteljivost hibrida kukuruza na prirodnu kontaminaciju
mikotoksinima (aflatoksin B1 (AFB1), deoksnivalenol (DON), zearalenol (ZEA) i fumonizini
(FB)). Hibrdi su gajeni tokom dve proizvodne godine (2019. i 2020) u dve lokacije.
Kontaminacija zrna kukuruza mikotoksinima ispitivana je na pet hibrida kukuruza šećerca. Nivo
kontaminacije proučavanih hibrida prvog klastera bio je uglavnom ispod prosečnih vrednosti
ZEA (sve vrednosti su bile nula) i DON. Vrednosti AFLA kod hibrida PK4 (S) 2020, PK6 (MS)
2020 i PK4 (MS) 2020 su bile ispod prosečne vrednosti, dok su vrednosti ovog mikotoksina bile
više od prosečnih vrednosti za ostale tretmane ovog klastera. Nivoi kontaminacije proučavanih
hibrida drugog klastera su uglavnom bili viši za mikotoksine ZEA i DON i niži za AFLA.
Fumnonizni nisu utrvđreni u uzorcima. Na kontaminaciju mikotoksinima značajno su uticali
hibiridi i godine. Utvrđeno je da su uslovi sredine uticali na nivoe DON i ZEA. Lokacija nije
značajno uticala na nivo AFLA kod hibrida kukuruza šećerca. Hibridi su značajno uticali na
variranje sadržaja mikotoksina (DON), dok hibrid × lokacija interakcija nije bila značajna.
Razlike su bile izraženije za sadržaj ZEA i ALFA nego za sadržaj DON. Najniži sadržaj DON
utvrđen je kod hibrida PK1, kod koga je sadržaj ZEA bio najviši. Analize mikotoksina pokazuju
da su nivoi AFLA, DON, ZEA i FB u svim ispitivanim hibridima bili ispod maksimalno
dozvoljenih nivoa koji su propisani zankonima Evropske Unije i Republike Srbije za kukuruz
koji je namenjen za direktnu ljudsku konzumaciju. Ovi rezultati potvrđuju da je osetljivost
hibrida jedan od važnih rizika pored klimatskih faktora za pojavu toksigenih gljiva i njihovih
mikotoksima. Tolerantnost genotipa je veoma važna preventivna mera, na koju oplemenjivači
moraju da obrate pažnju u programima oplemenjivanja kukuruza šećerca.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "The occurrence of mycotoxins in sweet maize hybrids",
volume = "53",
number = "3",
pages = "1311-1320",
doi = "10.2298/GENSR2103311N"
}
Nikolić, M., Srdić, J., Savić, I., Žilić, S., Stevanović, M., Kandić, V.,& Stanković, S.. (2021). The occurrence of mycotoxins in sweet maize hybrids. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1311-1320.
https://doi.org/10.2298/GENSR2103311N
Nikolić M, Srdić J, Savić I, Žilić S, Stevanović M, Kandić V, Stanković S. The occurrence of mycotoxins in sweet maize hybrids. in Genetika. 2021;53(3):1311-1320.
doi:10.2298/GENSR2103311N .
Nikolić, Milica, Srdić, Jelena, Savić, Iva, Žilić, Slađana, Stevanović, Milan, Kandić, Vesna, Stanković, Slavica, "The occurrence of mycotoxins in sweet maize hybrids" in Genetika, 53, no. 3 (2021):1311-1320,
https://doi.org/10.2298/GENSR2103311N . .
1

The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain

Savić, Iva; Nikolić, Milica; Vico, Ivana; Mladenović-Drinić, Snežana; Duduk, Natasa; Stanković, Slavica

(Beograd : Društvo genetičara Srbija, 2021)

TY  - JOUR
AU  - Savić, Iva
AU  - Nikolić, Milica
AU  - Vico, Ivana
AU  - Mladenović-Drinić, Snežana
AU  - Duduk, Natasa
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/830
AB  - During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.
AB  - Tokom perioda od 2014. do 2017. godine sakupljani su uzorci pšenice sa fuzarioznih klasova sa 20 lokacija u Srbiji. Nakon izolacije, gljive su gajene na KDA podlozi 7 dana u termostatu na temperaturi od 25oC. Na osnovu identifikacije in situ na KDA (krompir dekstrozni agar), 36 izolata sekcije Liseola odabrano je za dalje analize. Potvrđena je patogenost svih izolata na listovima pšenice. Virulentnost izolata utvrđena je merenjem dužina formirane pege na mestu inokulacije. Za dokazivanje prisustva vrste Fusarium verticillioides korišćen je par prajmera FV-F2/FV-R koji umnožava sekvence gaoB gena i koji se pokazao kao specifičan za navedenu vrstu. Takođe, sa istim ciljem korišćen je par prajmera VER1-VER2 dizajniran na osnovu dela genske sekvence za kalmodulin. Kao pozitivna kontrola korišćen je referentni izolat RBG 1603 Q27. Par prajmera VER1-VER2 obrazovao je traku očekivane veličine (578bp) kod 18 izolata, dok je pomoću para prajmera FV-F2/FV-R traka dužine 370bp potvrdila prisustvo F. verticillioides kod 16 izolata. Šesnaest od 18 izolata koji su identifikovani VER1-VER2 parom, takođe su identifikovani i FV-FS/FV-R prajmerima. U negativnoj kontroli nije bilo amplifikacije.
PB  - Beograd : Društvo genetičara Srbija
T2  - Genetika
T1  - The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain
VL  - 53
IS  - 2
SP  - 641
EP  - 649
DO  - 10.2298/GENSR2102641S
ER  - 
@article{
author = "Savić, Iva and Nikolić, Milica and Vico, Ivana and Mladenović-Drinić, Snežana and Duduk, Natasa and Stanković, Slavica",
year = "2021",
abstract = "During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control., Tokom perioda od 2014. do 2017. godine sakupljani su uzorci pšenice sa fuzarioznih klasova sa 20 lokacija u Srbiji. Nakon izolacije, gljive su gajene na KDA podlozi 7 dana u termostatu na temperaturi od 25oC. Na osnovu identifikacije in situ na KDA (krompir dekstrozni agar), 36 izolata sekcije Liseola odabrano je za dalje analize. Potvrđena je patogenost svih izolata na listovima pšenice. Virulentnost izolata utvrđena je merenjem dužina formirane pege na mestu inokulacije. Za dokazivanje prisustva vrste Fusarium verticillioides korišćen je par prajmera FV-F2/FV-R koji umnožava sekvence gaoB gena i koji se pokazao kao specifičan za navedenu vrstu. Takođe, sa istim ciljem korišćen je par prajmera VER1-VER2 dizajniran na osnovu dela genske sekvence za kalmodulin. Kao pozitivna kontrola korišćen je referentni izolat RBG 1603 Q27. Par prajmera VER1-VER2 obrazovao je traku očekivane veličine (578bp) kod 18 izolata, dok je pomoću para prajmera FV-F2/FV-R traka dužine 370bp potvrdila prisustvo F. verticillioides kod 16 izolata. Šesnaest od 18 izolata koji su identifikovani VER1-VER2 parom, takođe su identifikovani i FV-FS/FV-R prajmerima. U negativnoj kontroli nije bilo amplifikacije.",
publisher = "Beograd : Društvo genetičara Srbija",
journal = "Genetika",
title = "The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain",
volume = "53",
number = "2",
pages = "641-649",
doi = "10.2298/GENSR2102641S"
}
Savić, I., Nikolić, M., Vico, I., Mladenović-Drinić, S., Duduk, N.,& Stanković, S.. (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. in Genetika
Beograd : Društvo genetičara Srbija., 53(2), 641-649.
https://doi.org/10.2298/GENSR2102641S
Savić I, Nikolić M, Vico I, Mladenović-Drinić S, Duduk N, Stanković S. The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. in Genetika. 2021;53(2):641-649.
doi:10.2298/GENSR2102641S .
Savić, Iva, Nikolić, Milica, Vico, Ivana, Mladenović-Drinić, Snežana, Duduk, Natasa, Stanković, Slavica, "The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain" in Genetika, 53, no. 2 (2021):641-649,
https://doi.org/10.2298/GENSR2102641S . .

Effects of ascorbic acid and sugar on instrumental and sensory properties of composite breads

Simić, Marijana; Šimurina, Olivera; Nježić, Zvonko; Vančetović, Jelena; Kandić, Vesna; Nikolić, Valentina; Žilić, Slađana

(Novi Sad : University of Novi Sad, Institute of Food Technology (FINS), 2021)

TY  - JOUR
AU  - Simić, Marijana
AU  - Šimurina, Olivera
AU  - Nježić, Zvonko
AU  - Vančetović, Jelena
AU  - Kandić, Vesna
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/859
AB  - The present study was carried out to demonstrate the combined effects of different maize flour, ascorbic acid and sugar on the physical, textural and sensory properties of composite breads. The composite flour was prepared using 70% of wheat flour and 30% of flour obtained from grain of differently colored maize-light blue, blue, red and yellow maize flour. Chemical characterization of composite flours made with four different types of maize was also assessed. Furthermore, the content of total phenolics, flavonoids, anthocyanins, phenolic acids and antioxidant capacity in composite flours was determined. A total of 12 breads were prepared, four of which were control composite breads, four breads with ascorbic acid, and four were breads with ascorbic acid and sugar. The content of total phenolic compounds showed clear differences among all composite flours. The anthocyanins content determined in composite flours was in the following descending order: blue>red>light blue, while in the yellow maize composite flour anthocyanins were not detected. The results showed that the addition of AsA (0.025%) and sugar (5%) negatively affected the volume as well as the specific volume of composite wheat-maize breads. The texture analysis showed that the addition of AsA in the amount of 0.025% had no impact on springiness, cohesiveness and resilience of bread crumb, while it increased crumb hardness. However, composite breads made with AsA and AsA/sugar showed a more compact structure, with a larger number of cells and smaller mean cell areas. AsA/sugar bread samples within the tested doses had the lowest springiness, which is indicative of brittleness and reflects the tendency of the bread to crumble when slicing. Results of the sensory evaluation revealed that the AsA and sugar addition had a generally positive effect on the investigated sensory attributes.
AB  - U ovom radu, ispitivan je zajednički uticaj brašna kukuruza različite boje zrna,
askorbinske kiseline i šećera na fizička, teksturna i senzorna svojstva mešanog pšenično-
kukuruznog hleba. Smeša brašna je pripremljena od 70% pšeničnog brašna i 30% brašna
dobijenog od različito obojenog kukuruznog zrna – svetloplavog, plavog, crvenog i žutog.
Izvršena je i hemijska karakterizacija pšenično-kukuruznih smeša brašna. U pšenično-
kukuruznim smešama brašna određen je sadržaj ukupnih fenola, flavonoida, antocijana,
fenolnih kiselina i antioksidativni kapacitet. Ukupno je pripremljeno 12 hlebova, od kojih
su četiri bila kontrolna, četiri sa askorbinskom kiselinom i četiri hleba sa askorbinskom
kiselinom i šećerom. Sadržaj ukupnih fenolnih jedinjenja pokazao je jasne razlike između
svih pšenično-kukuruznih smeša brašna. Sadržaj antocijana je u smešama pšenično-
kukuruznog brašna imao sledeći opadajući redosled: smeša sa plavim
kukuruzom>crvenim kukuruzom>svetlo plavim kukuruzom, dok u smeši pšeničnog
brašna i brašna žutog kukuruza antocijani nisu detektovani. Rezultati su pokazali da je
dodatak askorbinske kiseline (0,025%) i šećera (5%) negativno uticao na zapreminu kao i
na specifičnu zapreminu mešanih pšenično-kukuruznih hlebova. Analiza teksture je
pokazala da dodatak askorbinske kiseline u količini od 0,025% nije uticao na elastičnost,
kohezivnost i elastičnost sredine hleba, ali je povećao tvrdoću sredine. Međutim, mešani
pšenično-kukuruzni hlebovi sa dodatkom askorbinske kiseline i askorbinske kiseline i
šećera, su pokazali kompaktniju strukturu sa većim brojem pora. Uzorci hleba sa
askorbinskom kiselinom i šećerom u ispitivanim dozama imali su najmanju elastičnost,
što ukazuje na krtost i odražava se na mrvljenje hleba prilikom sečenja. Rezultati
senzorne analize pokazali su da je dodatak askorbinske kiseline i šećera generalno imao
pozitivan uticaj na ispitivana senzorna svojstva.
PB  - Novi Sad : University of Novi Sad, Institute of Food Technology (FINS)
PB  - Novi Sad : Univerzitet u Novom Sadu, Naučni institut za prehrambene tehnologije
T2  - Food and Feed Research
T1  - Effects of ascorbic acid and sugar on instrumental and sensory properties of composite breads
T1  - Uticaj askorbinske kiseline i šećera na fizička, teksturna i senzorna svojstva mešanih pšenično-kukuruznih hlebova
VL  - 48
IS  - 2
SP  - 185
EP  - 200
DO  - 10.5937/ffr48-34944
ER  - 
@article{
author = "Simić, Marijana and Šimurina, Olivera and Nježić, Zvonko and Vančetović, Jelena and Kandić, Vesna and Nikolić, Valentina and Žilić, Slađana",
year = "2021",
abstract = "The present study was carried out to demonstrate the combined effects of different maize flour, ascorbic acid and sugar on the physical, textural and sensory properties of composite breads. The composite flour was prepared using 70% of wheat flour and 30% of flour obtained from grain of differently colored maize-light blue, blue, red and yellow maize flour. Chemical characterization of composite flours made with four different types of maize was also assessed. Furthermore, the content of total phenolics, flavonoids, anthocyanins, phenolic acids and antioxidant capacity in composite flours was determined. A total of 12 breads were prepared, four of which were control composite breads, four breads with ascorbic acid, and four were breads with ascorbic acid and sugar. The content of total phenolic compounds showed clear differences among all composite flours. The anthocyanins content determined in composite flours was in the following descending order: blue>red>light blue, while in the yellow maize composite flour anthocyanins were not detected. The results showed that the addition of AsA (0.025%) and sugar (5%) negatively affected the volume as well as the specific volume of composite wheat-maize breads. The texture analysis showed that the addition of AsA in the amount of 0.025% had no impact on springiness, cohesiveness and resilience of bread crumb, while it increased crumb hardness. However, composite breads made with AsA and AsA/sugar showed a more compact structure, with a larger number of cells and smaller mean cell areas. AsA/sugar bread samples within the tested doses had the lowest springiness, which is indicative of brittleness and reflects the tendency of the bread to crumble when slicing. Results of the sensory evaluation revealed that the AsA and sugar addition had a generally positive effect on the investigated sensory attributes., U ovom radu, ispitivan je zajednički uticaj brašna kukuruza različite boje zrna,
askorbinske kiseline i šećera na fizička, teksturna i senzorna svojstva mešanog pšenično-
kukuruznog hleba. Smeša brašna je pripremljena od 70% pšeničnog brašna i 30% brašna
dobijenog od različito obojenog kukuruznog zrna – svetloplavog, plavog, crvenog i žutog.
Izvršena je i hemijska karakterizacija pšenično-kukuruznih smeša brašna. U pšenično-
kukuruznim smešama brašna određen je sadržaj ukupnih fenola, flavonoida, antocijana,
fenolnih kiselina i antioksidativni kapacitet. Ukupno je pripremljeno 12 hlebova, od kojih
su četiri bila kontrolna, četiri sa askorbinskom kiselinom i četiri hleba sa askorbinskom
kiselinom i šećerom. Sadržaj ukupnih fenolnih jedinjenja pokazao je jasne razlike između
svih pšenično-kukuruznih smeša brašna. Sadržaj antocijana je u smešama pšenično-
kukuruznog brašna imao sledeći opadajući redosled: smeša sa plavim
kukuruzom>crvenim kukuruzom>svetlo plavim kukuruzom, dok u smeši pšeničnog
brašna i brašna žutog kukuruza antocijani nisu detektovani. Rezultati su pokazali da je
dodatak askorbinske kiseline (0,025%) i šećera (5%) negativno uticao na zapreminu kao i
na specifičnu zapreminu mešanih pšenično-kukuruznih hlebova. Analiza teksture je
pokazala da dodatak askorbinske kiseline u količini od 0,025% nije uticao na elastičnost,
kohezivnost i elastičnost sredine hleba, ali je povećao tvrdoću sredine. Međutim, mešani
pšenično-kukuruzni hlebovi sa dodatkom askorbinske kiseline i askorbinske kiseline i
šećera, su pokazali kompaktniju strukturu sa većim brojem pora. Uzorci hleba sa
askorbinskom kiselinom i šećerom u ispitivanim dozama imali su najmanju elastičnost,
što ukazuje na krtost i odražava se na mrvljenje hleba prilikom sečenja. Rezultati
senzorne analize pokazali su da je dodatak askorbinske kiseline i šećera generalno imao
pozitivan uticaj na ispitivana senzorna svojstva.",
publisher = "Novi Sad : University of Novi Sad, Institute of Food Technology (FINS), Novi Sad : Univerzitet u Novom Sadu, Naučni institut za prehrambene tehnologije",
journal = "Food and Feed Research",
title = "Effects of ascorbic acid and sugar on instrumental and sensory properties of composite breads, Uticaj askorbinske kiseline i šećera na fizička, teksturna i senzorna svojstva mešanih pšenično-kukuruznih hlebova",
volume = "48",
number = "2",
pages = "185-200",
doi = "10.5937/ffr48-34944"
}
Simić, M., Šimurina, O., Nježić, Z., Vančetović, J., Kandić, V., Nikolić, V.,& Žilić, S.. (2021). Effects of ascorbic acid and sugar on instrumental and sensory properties of composite breads. in Food and Feed Research
Novi Sad : University of Novi Sad, Institute of Food Technology (FINS)., 48(2), 185-200.
https://doi.org/10.5937/ffr48-34944
Simić M, Šimurina O, Nježić Z, Vančetović J, Kandić V, Nikolić V, Žilić S. Effects of ascorbic acid and sugar on instrumental and sensory properties of composite breads. in Food and Feed Research. 2021;48(2):185-200.
doi:10.5937/ffr48-34944 .
Simić, Marijana, Šimurina, Olivera, Nježić, Zvonko, Vančetović, Jelena, Kandić, Vesna, Nikolić, Valentina, Žilić, Slađana, "Effects of ascorbic acid and sugar on instrumental and sensory properties of composite breads" in Food and Feed Research, 48, no. 2 (2021):185-200,
https://doi.org/10.5937/ffr48-34944 . .

Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane

Nikolić, Valentina; Žilić, Slađana; Simić, Marijana; Vasić, Marko G.; Srdić, Jelena; Delić, Nenad; Delić, Nenad

(Novi Sad : National Society of Processing and Energy in Agriculture, 2021)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Žilić, Slađana
AU  - Simić, Marijana
AU  - Vasić, Marko G.
AU  - Srdić, Jelena
AU  - Delić, Nenad
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/846
AB  - Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane
T1  - Possibilities of maize hybrids utilization in canned baby corn production
VL  - 25
IS  - 1
SP  - 16
EP  - 19
DO  - 10.5937/jpea25-30887
ER  - 
@article{
author = "Nikolić, Valentina and Žilić, Slađana and Simić, Marijana and Vasić, Marko G. and Srdić, Jelena and Delić, Nenad and Delić, Nenad",
year = "2021",
abstract = "Five maize hybrids were used in this research: two yellow and one white dent, sweet hybrid, and yellow popcorn. Five brine
recipes with acetic acid were examined, of which two with the addition of potassium sorbate. The brines with preservatives were
stable for 16 months without colour changes of the liquid and corn cobs. The pH of brines ranged from 3.39 to 3.89. Canned hybrids
ZP 366 and ZP 611k in brine without sugar, and with the addition of potassium sorbate and potassium metabisulfite marked as
Number 5, showed the best sensory characteristics. The protein content determined in ZP 366 (9.56 %) and ZP 611k (10.23 %) did
not vary significantly compared to whole-grain maize flour, while crude fibre content (7.67 and 6.88 %), and ash content (21.96 and
20.72 %) were significantly higher than in flour (crude fibre: 2.40; 2.64 %, ash:1.35; 1.48 %, respectively). This research will be
continued in order to implement preliminary findings and new data on this subject",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture, Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane, Possibilities of maize hybrids utilization in canned baby corn production",
volume = "25",
number = "1",
pages = "16-19",
doi = "10.5937/jpea25-30887"
}
Nikolić, V., Žilić, S., Simić, M., Vasić, M. G., Srdić, J., Delić, N.,& Delić, N.. (2021). Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture
Novi Sad : National Society of Processing and Energy in Agriculture., 25(1), 16-19.
https://doi.org/10.5937/jpea25-30887
Nikolić V, Žilić S, Simić M, Vasić MG, Srdić J, Delić N, Delić N. Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane. in Journal on Processing and Energy in Agriculture. 2021;25(1):16-19.
doi:10.5937/jpea25-30887 .
Nikolić, Valentina, Žilić, Slađana, Simić, Marijana, Vasić, Marko G., Srdić, Jelena, Delić, Nenad, Delić, Nenad, "Mogućnosti primene hibrida kukuruza u proizvodnji baby corn konzervisane hrane" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021):16-19,
https://doi.org/10.5937/jpea25-30887 . .
1

Pasting properties and the baking functionality of whole-grain wheat flour with different amylose and dietary fibers content

Nikolić, Valentina; Simić, Marijana; Kandić, Vesna; Dodevska, Margarita; Titan, Primož; Dodig, Dejan; Žilić, Slađana

(New York : Wiley, 2021)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Simić, Marijana
AU  - Kandić, Vesna
AU  - Dodevska, Margarita
AU  - Titan, Primož
AU  - Dodig, Dejan
AU  - Žilić, Slađana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/841
AB  - Twenty-five varieties of wheat were used in this study in order to determine the effect of amylose and amylopectin, as well as dietary fibers on the pasting properties and baking functionality of the whole-grain flour. The results were compared to the properties of refined wheat flour. The analyzed samples were divided into six groups based on the amylose content: (1) 11%–14%, (2) 15%–17%, (3) 18%–19%, (4) high amylose (36.5% and 41%), (5) waxy (0%), and 6) refined flour (22%). Amylose influenced viscosity by large, however, variations in pasting properties can as well be attributed to the differences in the non-starch structural components. Variations in solvent retention capacities (SRC) of flours depended primarily on the content of different fiber fractions and proteins. The starch and amylose content had a positive effect on SRC for lactic acid and sucrose, respectively. Furthermore, pasting and functionality are complex properties conditioned by different flour constituents.
PB  - New York : Wiley
T2  - Journal of Food Processing and Preservation
T1  - Pasting properties and the baking functionality of whole-grain wheat flour with different amylose and dietary fibers content
SP  - 15805
DO  - 10.1111/jfpp.15805
ER  - 
@article{
author = "Nikolić, Valentina and Simić, Marijana and Kandić, Vesna and Dodevska, Margarita and Titan, Primož and Dodig, Dejan and Žilić, Slađana",
year = "2021",
abstract = "Twenty-five varieties of wheat were used in this study in order to determine the effect of amylose and amylopectin, as well as dietary fibers on the pasting properties and baking functionality of the whole-grain flour. The results were compared to the properties of refined wheat flour. The analyzed samples were divided into six groups based on the amylose content: (1) 11%–14%, (2) 15%–17%, (3) 18%–19%, (4) high amylose (36.5% and 41%), (5) waxy (0%), and 6) refined flour (22%). Amylose influenced viscosity by large, however, variations in pasting properties can as well be attributed to the differences in the non-starch structural components. Variations in solvent retention capacities (SRC) of flours depended primarily on the content of different fiber fractions and proteins. The starch and amylose content had a positive effect on SRC for lactic acid and sucrose, respectively. Furthermore, pasting and functionality are complex properties conditioned by different flour constituents.",
publisher = "New York : Wiley",
journal = "Journal of Food Processing and Preservation",
title = "Pasting properties and the baking functionality of whole-grain wheat flour with different amylose and dietary fibers content",
pages = "15805",
doi = "10.1111/jfpp.15805"
}
Nikolić, V., Simić, M., Kandić, V., Dodevska, M., Titan, P., Dodig, D.,& Žilić, S.. (2021). Pasting properties and the baking functionality of whole-grain wheat flour with different amylose and dietary fibers content. in Journal of Food Processing and Preservation
New York : Wiley., 15805.
https://doi.org/10.1111/jfpp.15805
Nikolić V, Simić M, Kandić V, Dodevska M, Titan P, Dodig D, Žilić S. Pasting properties and the baking functionality of whole-grain wheat flour with different amylose and dietary fibers content. in Journal of Food Processing and Preservation. 2021;:15805.
doi:10.1111/jfpp.15805 .
Nikolić, Valentina, Simić, Marijana, Kandić, Vesna, Dodevska, Margarita, Titan, Primož, Dodig, Dejan, Žilić, Slađana, "Pasting properties and the baking functionality of whole-grain wheat flour with different amylose and dietary fibers content" in Journal of Food Processing and Preservation (2021):15805,
https://doi.org/10.1111/jfpp.15805 . .

Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions

Božić, Manja; Stanojević, Aleksandra; Marković, Ksenija; Ignjatović-Micić, Dragana; Nikolić, Dragana; Milivojević, Marija; Nikolić, Ana

(Belgrade : Serbian Genetics Society, 2021)

TY  - JOUR
AU  - Božić, Manja
AU  - Stanojević, Aleksandra
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Dragana
AU  - Milivojević, Marija
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/832
AB  - Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance.
AB  - Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions
VL  - 53
IS  - 3
SP  - 1147
EP  - 1165
DO  - 10.2298/GENSR2103147B
ER  - 
@article{
author = "Božić, Manja and Stanojević, Aleksandra and Marković, Ksenija and Ignjatović-Micić, Dragana and Nikolić, Dragana and Milivojević, Marija and Nikolić, Ana",
year = "2021",
abstract = "Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance., Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions",
volume = "53",
number = "3",
pages = "1147-1165",
doi = "10.2298/GENSR2103147B"
}
Božić, M., Stanojević, A., Marković, K., Ignjatović-Micić, D., Nikolić, D., Milivojević, M.,& Nikolić, A.. (2021). Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1147-1165.
https://doi.org/10.2298/GENSR2103147B
Božić M, Stanojević A, Marković K, Ignjatović-Micić D, Nikolić D, Milivojević M, Nikolić A. Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika. 2021;53(3):1147-1165.
doi:10.2298/GENSR2103147B .
Božić, Manja, Stanojević, Aleksandra, Marković, Ksenija, Ignjatović-Micić, Dragana, Nikolić, Dragana, Milivojević, Marija, Nikolić, Ana, "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions" in Genetika, 53, no. 3 (2021):1147-1165,
https://doi.org/10.2298/GENSR2103147B . .

Antioxidants from maize seeds and accelerated ageing

Kravić, Natalija; Dragičević, Vesna; Milivojević, Marija; Babić, Vojka; Žilić, Slađana

(Beograd : Društvo selekcionara i semenara Republike Srbije, 2021)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Dragičević, Vesna
AU  - Milivojević, Marija
AU  - Babić, Vojka
AU  - Žilić, Slađana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/839
AB  - Seed deterioration is expressed as the loss of viability, vigour and quality during ageing The
major causes of seed deterioration refer to free radical-mediated lipid peroxidation, enzyme
inactivation or protein degradation, disruption of cellular membranes and damage to genetic
integrity Accelerated ageing (AA) is a simple method for studying the mechanisms of deterio-
ration over short periods In order to estimate the deterioration rate, seeds of four maize geno-
types were exposed to accelerated ageing for different time points (three and six days of AA) In
parallel, the same genotypes were evaluated regarding the content of total phenolics, phenolic
acids, flavonoids and anthocyanins, as well as regarding total antioxidant capacity Compared
to non-stressed seeds, ageing was evidenced by decreased germination energy (48 9%), a total
number of seedlings (40 8%) and seedlings growth (70 0% for root length, 44 0% and 10 5% for
root and shoot fresh weight, 5 0% for seed rest fresh weight, 12 0% and 11 5% for root and shoot
dry weight, 15 3% for seed rest dry weight, respectively), as well as by an increased number of
abnormal seedlings (22 0%) Correlation analysis revealed that higher content of total phenolics,
flavonoids and anthocyanins contributed to a higher radical scavenging activity, which was re-
flected through a lower level of deterioration rate, both regarding the energy of germination and
overall seedling performance.
AB  - Propadanje semena se ogleda u gubitku životne sposobnosti, energije i kvaliteta Dejstvo slo-
bodnih radikala dovodi do peroksidacije lipida, inaktivacije enzima, degradacije proteina, raza-
ranja ćelijskih membrana i oštećenja genetičkog materijala, i smatra se glavnim uzrokom propa-
danja semena Ubrzano starenje, kao metod, omogućava proučavanje mehanizama propadanja
semena u kratkom vremenskom periodu Za procenu stepena propadanja, seme četiri genotipa
kukuruza je izloženo tretmanu ubrzanog starenja u trajanju od tri i šest dana Uporedo sa ovim,
kod istih genotipova je ispitivan sadržaj ukupnih fenola, fenolnih kiselina, flavonoida i antoci-
jana, kao i ukupni antioksidativni kapacitet Poređenjem sa nestresiranim semenom (kontrola),
starenje je rezultiralo padom energije klijavosti (48,9%), smanjenjem ukupnog broja klijanaca
(40,8%), smanjenjem rasta klijanaca (70,0% za dužinu korena, 44,0% i 10,5% za svežu masu ko-
rena i izdanka, 5,0% za svežu masu semenog ostatka, 12 0% i 11 5% za suvu masu korena i iz -
danka, 15 3% za suvu masu semenog ostatka, respektivno), kao i povećanjem broja nenormalnih
klijanaca (22,0%) Korelacionom analizom je utvrđeno da je povećani sadržaj ukupnih fenola,
flavonoida i antocijana doprineo većoj sposobnosti gušenja slobodnih radikala, reflektujući se
kroz smanjen stepen propadanja semena, kako u pogledu energije klijavosti, tako i u pogledu
ukupne performanse klijanca.
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Antioxidants from maize seeds and accelerated ageing
VL  - 27
IS  - 2
SP  - 47
EP  - 57
DO  - 10.5937/SelSem2102047K
ER  - 
@article{
author = "Kravić, Natalija and Dragičević, Vesna and Milivojević, Marija and Babić, Vojka and Žilić, Slađana",
year = "2021",
abstract = "Seed deterioration is expressed as the loss of viability, vigour and quality during ageing The
major causes of seed deterioration refer to free radical-mediated lipid peroxidation, enzyme
inactivation or protein degradation, disruption of cellular membranes and damage to genetic
integrity Accelerated ageing (AA) is a simple method for studying the mechanisms of deterio-
ration over short periods In order to estimate the deterioration rate, seeds of four maize geno-
types were exposed to accelerated ageing for different time points (three and six days of AA) In
parallel, the same genotypes were evaluated regarding the content of total phenolics, phenolic
acids, flavonoids and anthocyanins, as well as regarding total antioxidant capacity Compared
to non-stressed seeds, ageing was evidenced by decreased germination energy (48 9%), a total
number of seedlings (40 8%) and seedlings growth (70 0% for root length, 44 0% and 10 5% for
root and shoot fresh weight, 5 0% for seed rest fresh weight, 12 0% and 11 5% for root and shoot
dry weight, 15 3% for seed rest dry weight, respectively), as well as by an increased number of
abnormal seedlings (22 0%) Correlation analysis revealed that higher content of total phenolics,
flavonoids and anthocyanins contributed to a higher radical scavenging activity, which was re-
flected through a lower level of deterioration rate, both regarding the energy of germination and
overall seedling performance., Propadanje semena se ogleda u gubitku životne sposobnosti, energije i kvaliteta Dejstvo slo-
bodnih radikala dovodi do peroksidacije lipida, inaktivacije enzima, degradacije proteina, raza-
ranja ćelijskih membrana i oštećenja genetičkog materijala, i smatra se glavnim uzrokom propa-
danja semena Ubrzano starenje, kao metod, omogućava proučavanje mehanizama propadanja
semena u kratkom vremenskom periodu Za procenu stepena propadanja, seme četiri genotipa
kukuruza je izloženo tretmanu ubrzanog starenja u trajanju od tri i šest dana Uporedo sa ovim,
kod istih genotipova je ispitivan sadržaj ukupnih fenola, fenolnih kiselina, flavonoida i antoci-
jana, kao i ukupni antioksidativni kapacitet Poređenjem sa nestresiranim semenom (kontrola),
starenje je rezultiralo padom energije klijavosti (48,9%), smanjenjem ukupnog broja klijanaca
(40,8%), smanjenjem rasta klijanaca (70,0% za dužinu korena, 44,0% i 10,5% za svežu masu ko-
rena i izdanka, 5,0% za svežu masu semenog ostatka, 12 0% i 11 5% za suvu masu korena i iz -
danka, 15 3% za suvu masu semenog ostatka, respektivno), kao i povećanjem broja nenormalnih
klijanaca (22,0%) Korelacionom analizom je utvrđeno da je povećani sadržaj ukupnih fenola,
flavonoida i antocijana doprineo većoj sposobnosti gušenja slobodnih radikala, reflektujući se
kroz smanjen stepen propadanja semena, kako u pogledu energije klijavosti, tako i u pogledu
ukupne performanse klijanca.",
publisher = "Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Antioxidants from maize seeds and accelerated ageing",
volume = "27",
number = "2",
pages = "47-57",
doi = "10.5937/SelSem2102047K"
}
Kravić, N., Dragičević, V., Milivojević, M., Babić, V.,& Žilić, S.. (2021). Antioxidants from maize seeds and accelerated ageing. in Selekcija i semenarstvo
Beograd : Društvo selekcionara i semenara Republike Srbije., 27(2), 47-57.
https://doi.org/10.5937/SelSem2102047K
Kravić N, Dragičević V, Milivojević M, Babić V, Žilić S. Antioxidants from maize seeds and accelerated ageing. in Selekcija i semenarstvo. 2021;27(2):47-57.
doi:10.5937/SelSem2102047K .
Kravić, Natalija, Dragičević, Vesna, Milivojević, Marija, Babić, Vojka, Žilić, Slađana, "Antioxidants from maize seeds and accelerated ageing" in Selekcija i semenarstvo, 27, no. 2 (2021):47-57,
https://doi.org/10.5937/SelSem2102047K . .