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Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality

Tabaković, Marijenka; Brankov, Milan; Dragičević, Vesna; Stanisavljević, Rade; Poštić, Dobrivoj; Rakić, Sveto; Oro, Violeta

(Novi Sad : National Society of Processing and Energy in Agriculture, 2023)

TY  - GEN
AU  - Tabaković, Marijenka
AU  - Brankov, Milan
AU  - Dragičević, Vesna
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Rakić, Sveto
AU  - Oro, Violeta
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1157
AB  - Natural allelopathic substances are products or metabolites of some plants, released into the environment, and they have
the potential to be used in sustainable agricultural management systems. A sustainable system includes the use of natural
resources for plant protection, diseases, pests, and weed control without the use of synthetic chemicals. Essential oils
(Eos) are well-known metabolites utilised in agriculture.
From previous studies, it was known that Eos have toxic effects on phytopathogens as well as an inhibitory effect on seed
germination. Less research has been done on the Eo's ability to reduce dormancy and boost germination. The study's
objective was to ascertain the significance of Eo's impact on alfalfa (Medicago sativa L.) seed dormancy.
The seed material of four different varieties of Medicago sativa was used for the experiment (Zaječarka, Banatska, K-28,
Novosadska). Two essential oils, lavender (Lavandula angustifolia Mill.) and mint (Mentha piperita L.) were applied to
seeds at four concentrations—1%, 0.5%, 0.2%, and 0.02%—along with water as a control. Lavender and mint essential
oils used in the experiment are commercial oils obtained from mint leaves and fresh lavender flowers by distillation.
Germination, dormant seeds and dead seeds were evaluated in a laboratory setting according to ISTA rules, in Petry dishes
on filter paper. A germination cabinet was the equipment for seed germination.
Results showed that the type of Eos had no discernible influence on germinated seeds, dormancy, and dead seeds. The oil
concentration was the most significant factor affecting physiological characteristics. Both oils in concentrations of -1%
and 0.5% inhibited seed germination 100%. The maximum germination at 91,66% was achieved with the Novosadska
variety using lavender oil at a concentration of 0.02%, while dormancy was reduced. Varieties Zaječarka and Banatska
had the highest level of dead and dormant seeds, when lavender and mint Eos were applied at a concentration of 0.2%.
This study showed that both Eos at a concentration of 0.02% had a stimulatory effect on seed germination and
simultaneously reduced seed dormancy, emphasizing their potential use for seed quality improvement in organic farming.
AB  - Prirodne alelopatske supstance su proizvodi ili metaboliti nekih biljaka, koje biljka otpušta u životnu sredinu, i imaju
potencijal da se koriste u upravljanju sistema održive poljoprivrede. Održiv sistem uključuje korišćenje prirodnih resursa
za zaštitu bilja, suzbijanje bolesti, štetočina i korova bez upotrebe sintetičkih hemikalija. Esencijalna ulja (Eu) su dobro
poznati metaboliti koji se koriste u poljoprivredi.
Iz ranijih istraživanja poznato je da Eu imaju toksično dejstvo na fitopatogene, kao i inhibitorno dejstvo na klijanje
semena. Manje istraživanja je rađeno o sposobnosti Eu da smanje mirovanje i povećaju klijavost semena. Cilj studije je
bio da se utvrdi značaj delovanja Eu na mirovanje (dormantnost) semena lucerke (Medicago sativa L.).
Za ogled korišćen je semenski materijal četiri sorte lucerke (Zaječarka, Banatska, K-28, Novosadska). Dva Eu, lavanda
(Lavandula angustifolia Mill.) i nana (Mentha piperita L.) primenjena su na semenu lucerke u četiri koncentracije — 1%,
0,5%, 0,2% i 0,02% — zajedno sa vodom kao kontrolom. Ulja nane i lavande korišćena u eksperimentu su komercijalna
ulja dobijena destilacijom listova nane i svežih cvetova lavande. Klijavost, dormantnost i mrtvo seme su procenjeni u
laboratorijskim uslovima prema ISTA pravilima, u petrijevim posudama na filter papiru. Klijanje je rađeno u komori za
klijanje.
Rezultati su pokazali da vrsta Eu nije imala vidljiv uticaj na klijanje semena, mirovanje i mrtvo seme. Koncentracija ulja
je bila najznačajniji faktor koji je uticao na fiziološke karakteristike. Oba ulja u koncentracijama od 1% i 0,5% su
inhibirala klijanje 100%. Maksimalna klijavost 91,66% je postignuta kod sorte Novosadska uz korišćenje ulja lavande u
koncentraciji 0,02%, dok se mirovanje smanjilo. Sorte Zaječarka i Banatska su imali najviši nivo mrtvog i dormantnog
semena, kada su tretirane uljem lavande i nane u koncentraciji od 0,2%.
Ova studija je pokazala da su oba Eu u koncentraciji od 0,02% imala stimulativni efekat na klijavost semena, i istovremeno
smanjila mirovanje semena, naglašavajući njihovu potencijalnu primenu za poboljšanje kvaliteta semena u organskoj
poljoprivredi.
PB  - Novi Sad : National Society of Processing and Energy in Agriculture
T2  - I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur
T1  - Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality
T1  - Primena esencijalnih ulja lavande i nane za poboljšanje kvaliteta semena lucerke (Medicago sativa L.)
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_rik_1157
ER  - 
@misc{
author = "Tabaković, Marijenka and Brankov, Milan and Dragičević, Vesna and Stanisavljević, Rade and Poštić, Dobrivoj and Rakić, Sveto and Oro, Violeta",
year = "2023",
abstract = "Natural allelopathic substances are products or metabolites of some plants, released into the environment, and they have
the potential to be used in sustainable agricultural management systems. A sustainable system includes the use of natural
resources for plant protection, diseases, pests, and weed control without the use of synthetic chemicals. Essential oils
(Eos) are well-known metabolites utilised in agriculture.
From previous studies, it was known that Eos have toxic effects on phytopathogens as well as an inhibitory effect on seed
germination. Less research has been done on the Eo's ability to reduce dormancy and boost germination. The study's
objective was to ascertain the significance of Eo's impact on alfalfa (Medicago sativa L.) seed dormancy.
The seed material of four different varieties of Medicago sativa was used for the experiment (Zaječarka, Banatska, K-28,
Novosadska). Two essential oils, lavender (Lavandula angustifolia Mill.) and mint (Mentha piperita L.) were applied to
seeds at four concentrations—1%, 0.5%, 0.2%, and 0.02%—along with water as a control. Lavender and mint essential
oils used in the experiment are commercial oils obtained from mint leaves and fresh lavender flowers by distillation.
Germination, dormant seeds and dead seeds were evaluated in a laboratory setting according to ISTA rules, in Petry dishes
on filter paper. A germination cabinet was the equipment for seed germination.
Results showed that the type of Eos had no discernible influence on germinated seeds, dormancy, and dead seeds. The oil
concentration was the most significant factor affecting physiological characteristics. Both oils in concentrations of -1%
and 0.5% inhibited seed germination 100%. The maximum germination at 91,66% was achieved with the Novosadska
variety using lavender oil at a concentration of 0.02%, while dormancy was reduced. Varieties Zaječarka and Banatska
had the highest level of dead and dormant seeds, when lavender and mint Eos were applied at a concentration of 0.2%.
This study showed that both Eos at a concentration of 0.02% had a stimulatory effect on seed germination and
simultaneously reduced seed dormancy, emphasizing their potential use for seed quality improvement in organic farming., Prirodne alelopatske supstance su proizvodi ili metaboliti nekih biljaka, koje biljka otpušta u životnu sredinu, i imaju
potencijal da se koriste u upravljanju sistema održive poljoprivrede. Održiv sistem uključuje korišćenje prirodnih resursa
za zaštitu bilja, suzbijanje bolesti, štetočina i korova bez upotrebe sintetičkih hemikalija. Esencijalna ulja (Eu) su dobro
poznati metaboliti koji se koriste u poljoprivredi.
Iz ranijih istraživanja poznato je da Eu imaju toksično dejstvo na fitopatogene, kao i inhibitorno dejstvo na klijanje
semena. Manje istraživanja je rađeno o sposobnosti Eu da smanje mirovanje i povećaju klijavost semena. Cilj studije je
bio da se utvrdi značaj delovanja Eu na mirovanje (dormantnost) semena lucerke (Medicago sativa L.).
Za ogled korišćen je semenski materijal četiri sorte lucerke (Zaječarka, Banatska, K-28, Novosadska). Dva Eu, lavanda
(Lavandula angustifolia Mill.) i nana (Mentha piperita L.) primenjena su na semenu lucerke u četiri koncentracije — 1%,
0,5%, 0,2% i 0,02% — zajedno sa vodom kao kontrolom. Ulja nane i lavande korišćena u eksperimentu su komercijalna
ulja dobijena destilacijom listova nane i svežih cvetova lavande. Klijavost, dormantnost i mrtvo seme su procenjeni u
laboratorijskim uslovima prema ISTA pravilima, u petrijevim posudama na filter papiru. Klijanje je rađeno u komori za
klijanje.
Rezultati su pokazali da vrsta Eu nije imala vidljiv uticaj na klijanje semena, mirovanje i mrtvo seme. Koncentracija ulja
je bila najznačajniji faktor koji je uticao na fiziološke karakteristike. Oba ulja u koncentracijama od 1% i 0,5% su
inhibirala klijanje 100%. Maksimalna klijavost 91,66% je postignuta kod sorte Novosadska uz korišćenje ulja lavande u
koncentraciji 0,02%, dok se mirovanje smanjilo. Sorte Zaječarka i Banatska su imali najviši nivo mrtvog i dormantnog
semena, kada su tretirane uljem lavande i nane u koncentraciji od 0,2%.
Ova studija je pokazala da su oba Eu u koncentraciji od 0,02% imala stimulativni efekat na klijavost semena, i istovremeno
smanjila mirovanje semena, naglašavajući njihovu potencijalnu primenu za poboljšanje kvaliteta semena u organskoj
poljoprivredi.",
publisher = "Novi Sad : National Society of Processing and Energy in Agriculture",
journal = "I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur",
title = "Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality, Primena esencijalnih ulja lavande i nane za poboljšanje kvaliteta semena lucerke (Medicago sativa L.)",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_rik_1157"
}
Tabaković, M., Brankov, M., Dragičević, V., Stanisavljević, R., Poštić, D., Rakić, S.,& Oro, V.. (2023). Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality. in I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur
Novi Sad : National Society of Processing and Energy in Agriculture., 139.
https://hdl.handle.net/21.15107/rcub_rik_1157
Tabaković M, Brankov M, Dragičević V, Stanisavljević R, Poštić D, Rakić S, Oro V. Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality. in I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur. 2023;:139.
https://hdl.handle.net/21.15107/rcub_rik_1157 .
Tabaković, Marijenka, Brankov, Milan, Dragičević, Vesna, Stanisavljević, Rade, Poštić, Dobrivoj, Rakić, Sveto, Oro, Violeta, "Application of lavender and mint essential oils for improvement of alfalfa (Medicago sativa L.) seed quality" in I N O P T E P 2023. and. XXXV Scientific-professional conference. process technology and energy in agricultur (2023):139,
https://hdl.handle.net/21.15107/rcub_rik_1157 .

Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines

Tabaković, Marijenka; Dragicevic, Vesna; Simic, Milena; Stanisavljević, Rade; Poštić, Dobrivoj; Brankov, Milan; Oro, Violeta

(Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi, 2022)

TY  - JOUR
AU  - Tabaković, Marijenka
AU  - Dragicevic, Vesna
AU  - Simic, Milena
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Brankov, Milan
AU  - Oro, Violeta
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1163
AB  - This study aimed to evaluate the influence of environmental conditions and genotype on the yield of three maize lines and the morphological properties of ears and seeds. Three hybrid maize lines were used as material in the study conducted in two consecutive years (2018 and 2019) at one location (Zemun Polje, Belgrade, Serbia). The following parameters were monitored: seed weight (SW), seed volume (V), bulk density (BD), ear length (EL), ear thickness (ET), fraction content and seed yield (MSY). Calibration divided the seed into a small seed fraction of 6.5-8.4 mm (SF) and a large seed fraction of 8.5-11 mm (LF). Seeds smaller than 6.5 mm and larger than 11 mm are presented as seed waste fraction (FW). The average seed yield for all three lines in the first and the second year was 6.13 and 4.66 t ha-1 , respectively. A significant impact of the environment was noticed on seed weights in both years (2018 and 2019), i.e. 327.56 and 251.77 g, respectively. The total variation in yield determined by the morphological characteristics of the seed was R2 =0.514.
AB  - Cilj rada bio je da se oceni uticaj uslova sredine i genotipa na prinos tri linije kukuruza i morfološke osobine klipa. U radu je
korišćeno seme tri linije kukuruza, u dvogodišnjem ogledu izvedenom u 2018 i 2019 godini, na jednoj lokaciji. Parametri koji su
posmatrani bili su prinos zrna (MSY), apsolutna masa semena (SW), zapremina semena (V), gustina semena (BD), dužina klipa (EL),
debljina klipa (ET) i težina klipa (EW). Kalibracijom semena dobijene su tri frakcije semena: sitna, veličine semena 6,5-8,4mm (SF),
krupna, veličine semena 8,5-11mm (KF) i frakcija otpada, veličine semena ispod 6,5mm i iznad 11mm (FW).Prosečan prinos za sve
linije u prvoj godine bio je 6,13 a u drugoj 4,66 t ha-1. Značajan efekat ekoloških uslova bio je i na apsolutnu masu semena (327,56 g
u prvoj; odnosno 251,77 g u drugoj godini). Ovako velika razlika u apsolutnoj masi semena odrazila se i na ukupan odnos sitne (SF)
i krupne frakcije (LF) semena na klipu. Ukupno variranje prinosa klipa determinisano morfološkim osobinama semena bilo je R2
 =
0.514. Sve morfološke osobine klipa i semena međusobno su značajno korelirale na oba nivoa značajnosti (p≤0,01, p≤0,05).
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines
VL  - 26
IS  - 2
SP  - 71
EP  - 74
DO  - 10.5937/jpea26-36609
ER  - 
@article{
author = "Tabaković, Marijenka and Dragicevic, Vesna and Simic, Milena and Stanisavljević, Rade and Poštić, Dobrivoj and Brankov, Milan and Oro, Violeta",
year = "2022",
abstract = "This study aimed to evaluate the influence of environmental conditions and genotype on the yield of three maize lines and the morphological properties of ears and seeds. Three hybrid maize lines were used as material in the study conducted in two consecutive years (2018 and 2019) at one location (Zemun Polje, Belgrade, Serbia). The following parameters were monitored: seed weight (SW), seed volume (V), bulk density (BD), ear length (EL), ear thickness (ET), fraction content and seed yield (MSY). Calibration divided the seed into a small seed fraction of 6.5-8.4 mm (SF) and a large seed fraction of 8.5-11 mm (LF). Seeds smaller than 6.5 mm and larger than 11 mm are presented as seed waste fraction (FW). The average seed yield for all three lines in the first and the second year was 6.13 and 4.66 t ha-1 , respectively. A significant impact of the environment was noticed on seed weights in both years (2018 and 2019), i.e. 327.56 and 251.77 g, respectively. The total variation in yield determined by the morphological characteristics of the seed was R2 =0.514., Cilj rada bio je da se oceni uticaj uslova sredine i genotipa na prinos tri linije kukuruza i morfološke osobine klipa. U radu je
korišćeno seme tri linije kukuruza, u dvogodišnjem ogledu izvedenom u 2018 i 2019 godini, na jednoj lokaciji. Parametri koji su
posmatrani bili su prinos zrna (MSY), apsolutna masa semena (SW), zapremina semena (V), gustina semena (BD), dužina klipa (EL),
debljina klipa (ET) i težina klipa (EW). Kalibracijom semena dobijene su tri frakcije semena: sitna, veličine semena 6,5-8,4mm (SF),
krupna, veličine semena 8,5-11mm (KF) i frakcija otpada, veličine semena ispod 6,5mm i iznad 11mm (FW).Prosečan prinos za sve
linije u prvoj godine bio je 6,13 a u drugoj 4,66 t ha-1. Značajan efekat ekoloških uslova bio je i na apsolutnu masu semena (327,56 g
u prvoj; odnosno 251,77 g u drugoj godini). Ovako velika razlika u apsolutnoj masi semena odrazila se i na ukupan odnos sitne (SF)
i krupne frakcije (LF) semena na klipu. Ukupno variranje prinosa klipa determinisano morfološkim osobinama semena bilo je R2
 =
0.514. Sve morfološke osobine klipa i semena međusobno su značajno korelirale na oba nivoa značajnosti (p≤0,01, p≤0,05).",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines",
volume = "26",
number = "2",
pages = "71-74",
doi = "10.5937/jpea26-36609"
}
Tabaković, M., Dragicevic, V., Simic, M., Stanisavljević, R., Poštić, D., Brankov, M.,& Oro, V.. (2022). Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines. in Journal on Processing and Energy in Agriculture
Novi Sad : Nacionalno društvo za procesnu tehniku u poljoprivredi., 26(2), 71-74.
https://doi.org/10.5937/jpea26-36609
Tabaković M, Dragicevic V, Simic M, Stanisavljević R, Poštić D, Brankov M, Oro V. Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines. in Journal on Processing and Energy in Agriculture. 2022;26(2):71-74.
doi:10.5937/jpea26-36609 .
Tabaković, Marijenka, Dragicevic, Vesna, Simic, Milena, Stanisavljević, Rade, Poštić, Dobrivoj, Brankov, Milan, Oro, Violeta, "Joint and direct effects of genotypes and environment conditions on yield and yield components variability of maize inbred lines" in Journal on Processing and Energy in Agriculture, 26, no. 2 (2022):71-74,
https://doi.org/10.5937/jpea26-36609 . .
1