TY  - JOUR
AU  - Djordjevic Melnik, Olivera
AU  - Božinović, Sofija
AU  - Vukadinović, Radmila
AU  - Milivojević, Marija
AU  - Petrović, Tanja
AU  - Živanović, Tomislav
AU  - Vančetović, Jelena
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1303
AB  - Five direct and reciprocal early hybrids, five direct and reciprocal late hybrids and a total
of their 12 parental inbred lines were used in order to test reciprocal effects among
hybrids in early germination phase and seed quality of their parental inbred lines. Five
hybrids were of early maturity FAO 300-400 group, and five of late maturity FAO 500-
600 group. In the early group one line of Lancaster origin (L1) was in common, and in
direct crosses used as male parent, while in reciprocal crosses as female parent. The same
case was with late hybrids where another Lancaster line (L2) was in common. In 2015
and 2016 all the material was multiplied in hand pollination and after six months of
storage three tests according to ISTA standards were performed: standard germination test
(SGT), cold-test (CT) and vigor radicle test (RE). According to the Analysis of variance,
a strong reciprocal effect was found among early hybrids for four out of five parameters
in RE and SGT, while it was found only for RE in late hybrids. However, in CT none of
the parameters had significant reciprocal effect. Two tested Lancaster inbreds were
superior in the hybrids over non-Lancaster lines when used as maternal components,
while as lines per se they did not display such superiority. Mid-parent heterosis (MPH)
ranged from -62.32% to 211.11% in RE test, with an average of 44.26%; from -10.15% to
17.93% in SGT test, with an average of 6.27%, and from -5.31% to 4.49% in CT, with an
average of 0.64%. Significant differences of MPH were found for nine pairs of reciprocal
hybrids for RE, three pairs for SGT and seven pairs for CT. This shows that reciprocal
differences in MPH were higher as the applied level of stress during early seed growth
raised. All the results showed very complicated mode of inheritance of tested traits in the
early developmental stages of young plants, including the phenomenon of heterosis which
is expressed already at the germination stage.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika
T1  - Seed quality of reciprocal maize single crosses and their parental inbred lines
VL  - 55
IS  - 2
SP  - 611
EP  - 629
DO  - 10.2298/GENSR2302611D
ER  - 

@article{
author = "Djordjevic Melnik, Olivera and Božinović, Sofija and Vukadinović, Radmila and Milivojević, Marija and Petrović, Tanja and Živanović, Tomislav and Vančetović, Jelena",
year = "2023",
abstract = "Five direct and reciprocal early hybrids, five direct and reciprocal late hybrids and a total
of their 12 parental inbred lines were used in order to test reciprocal effects among
hybrids in early germination phase and seed quality of their parental inbred lines. Five
hybrids were of early maturity FAO 300-400 group, and five of late maturity FAO 500-
600 group. In the early group one line of Lancaster origin (L1) was in common, and in
direct crosses used as male parent, while in reciprocal crosses as female parent. The same
case was with late hybrids where another Lancaster line (L2) was in common. In 2015
and 2016 all the material was multiplied in hand pollination and after six months of
storage three tests according to ISTA standards were performed: standard germination test
(SGT), cold-test (CT) and vigor radicle test (RE). According to the Analysis of variance,
a strong reciprocal effect was found among early hybrids for four out of five parameters
in RE and SGT, while it was found only for RE in late hybrids. However, in CT none of
the parameters had significant reciprocal effect. Two tested Lancaster inbreds were
superior in the hybrids over non-Lancaster lines when used as maternal components,
while as lines per se they did not display such superiority. Mid-parent heterosis (MPH)
ranged from -62.32% to 211.11% in RE test, with an average of 44.26%; from -10.15% to
17.93% in SGT test, with an average of 6.27%, and from -5.31% to 4.49% in CT, with an
average of 0.64%. Significant differences of MPH were found for nine pairs of reciprocal
hybrids for RE, three pairs for SGT and seven pairs for CT. This shows that reciprocal
differences in MPH were higher as the applied level of stress during early seed growth
raised. All the results showed very complicated mode of inheritance of tested traits in the
early developmental stages of young plants, including the phenomenon of heterosis which
is expressed already at the germination stage.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika",
title = "Seed quality of reciprocal maize single crosses and their parental inbred lines",
volume = "55",
number = "2",
pages = "611-629",
doi = "10.2298/GENSR2302611D"
}

Djordjevic Melnik, O., Božinović, S., Vukadinović, R., Milivojević, M., Petrović, T., Živanović, T.,& Vančetović, J.. (2023). Seed quality of reciprocal maize single crosses and their parental inbred lines. in Genetika
Beograd : Društvo genetičara Srbije., 55(2), 611-629.
https://doi.org/10.2298/GENSR2302611D

Djordjevic Melnik O, Božinović S, Vukadinović R, Milivojević M, Petrović T, Živanović T, Vančetović J. Seed quality of reciprocal maize single crosses and their parental inbred lines. in Genetika. 2023;55(2):611-629.
doi:10.2298/GENSR2302611D .

Djordjevic Melnik, Olivera, Božinović, Sofija, Vukadinović, Radmila, Milivojević, Marija, Petrović, Tanja, Živanović, Tomislav, Vančetović, Jelena, "Seed quality of reciprocal maize single crosses and their parental inbred lines" in Genetika, 55, no. 2 (2023):611-629,
https://doi.org/10.2298/GENSR2302611D . .

TY  - JOUR
AU  - Radenovic, Cedomir
AU  - Maksimov, Georgy
AU  - Kuramshina, Gulnara
AU  - Shutova, Vitalina
AU  - Hao, Jingrao
AU  - Delić, Nenad
AU  - Sečanski, Mile
AU  - Popović, Aleksandar
AU  - Bajuk-Bogdanovic, Danica
AU  - Radosavljević, Milica
AU  - Pavlov, Jovan
PY  - 2022
UR  - http://agrobiology.ru/5-2022radenovich-eng.html
UR  - http://rik.mrizp.rs/handle/123456789/1354
AB  - Инфракрасная (ИК) спектроскопия и спектроскопия комбинационного рассеяния (КР) — современные методы, на основе которых успешно формируются биотехнологические подходы, поз-воляющие проводить генетический и функциональный анализ отдельных органов и тканей растений на молекулярном уровне. В представленной работе с помощью спектроскопии поверхностного внут-реннего отражения (ПВО), которая представляет собой модификацию ИК-спектроскопии, впервые зарегистрированы и выявлены различия в содержании и конформации биомолекул в гомогенатах целого зерна гибридов кукурузы ZP 735 и его компонентов (эндосперм, перикарп, зародыш). Нашей целью была разработка методологии спектроскопии поверхностного внутреннего отражения для идентификации органических молекул, их содержания и конформации в семени кукурузы и его тканях. Объектом исследования служили зерна гибрида кукурузы (Zea mays L.) ZP 735 (оригина-тор — Maize Research Institute, Zemun Polje, г. Белград, Сербия). Методом случайной выборки было отобрано 30 зерен. Для получения ПВО-спектров образцы целых зерен и их частей (эндо-сперма, перикарпа и зародыша) измельчали, гомогенизировали и помещали в специальную ячейку прибора. Для регистрации ПВО-спектров использовали спектрометр (Nicolet™ iS20 FTIR Spectro-meter, «Thermo Scientific», США) с алмазом (diamond ATR crystal, «Thermo Scientific», США), для анализа спектров — пакет программ («Thermo Scientific», США). ПВО-спектры сопоставляли в библиотеке данных программы Advanced ATR correction Algorithm. Метод ПВО-спектроскопии ос-нован на отражении пучка света на границе раздела двух фаз: фазы кристалла с высоким показа-телем преломления, входящего в состав прибора ПВО, и фазы исследуемого образца с более низ-ким показателем преломления. В процессе измерения луч света проникает на небольшую глубину в фазу образца, где частично поглощается. При последующих облучениях образца это явление повторяется, и в результате регистрируется ПВО-спектр. Доказано, что в диапазоне от 400 см1 до 4000 см1 ПВО-спектры целого зерна, эндосперма, перикарпа и зародыша гибрида ZP 735 были аналогичны полученным ранее ИК-спектрам зерна, что свидетельствует о возможности использо-вания нового метода для технологий молекулярной селекции. Расположение полос ПВО-спектра характеризует различные формы колебаний валентных связей функциональных групп органических молекул семени, что позволяет идентифицировать не только наличие определенных молекул, но и их конформацию. Доказано, что с помощью анализа амплитуды полос ПВО-спектра (максималь-ная амплитуда интенсивности и высокая амплитуда интенсивности) в тканях семени можно кон-тролировать изменение содержания ряда органических соединений: протеинов, липидов, сахаров, сложных эфиров, амидов, кетонов, альдегидов, карбоновых кислот, простых эфиров, фенолов, спиртов, ароматических углеводородов, ациклических соединений, алкенов, алканов и алкинов. Полученные результаты важны для тестирования наличия в зерне необходимых органических со-единений или изменения их конформации в процессе селекции. К важным преимуществам метода ПВО-пектроскопии по сравнению с ИК-спектроскопией относятся, с одной стороны, сохранение нативности объекта (возможность проведения исследования без фиксации или окрашивания) и простая пробоподготовка, с другой — эффективная оценка содержания и конформации молекул с высокой чувствительностью (разрешение порядка 1,0 см1). Реализация разработанной методики для формирования технологии молекулярной селекции повысит рентабельность культивирования и эффективность селекции не только кукурузы, но и других сельскохозяйственных растений.
AB  - Infrared (IR) spectroscopy and Raman spectroscopy (RS) are modern methods on the basis of which biotechnological approaches are being successfully developed that allow genetic and func-tional analysis of individual plant organs and tissues at the molecular level. In the present work, using surface internal reflection spectroscopy (SIR), which is a modification of IR spectroscopy, differences in the content and conformation of biomolecules in grain homogenates and its components (endo-sperm, pericarp, germ) of ZP 735 maize hybrids were first recorded and revealed. Our goal was to develop a methodology for surface SIR for the identification of organic molecules, their content and conformation in corn seed and its tissues. The grains of the corn hybrid (Zea mays L.) ZP 735 (origi-nator Maize Research Institute, Zemun Polje, Belgrade, Serbia) served as the object of the study. Thirty grains were selected by random sampling. To obtain SIR spectra, samples of grains and their parts (endosperm, pericarp, and embryo) were crushed, homogenized, and placed in a special cell of the device. A spectrometer (FT-IR spectrophotometer, Thermo Scientific, USA) with diamond (dia-mond ATR crystal, Thermo Scientific, USA) was used to record the SIR spectra, and a software package (Thermo Scientific™ ) was used to analyze the spectra. The SIR spectra were compared in the data library of the Advanced ATR correction Algorithm program. The SIR spectroscopy method is based on the reflection of a light beam at the interface between two phases: the phase of a crystal with a high refractive index, which is part of the SIR device, and the phase of the sample under study with a lower refractive index. During the measurement, the light beam penetrates to a small depth into the phase of the sample, where it is partially absorbed. During subsequent irradiation of the sample, this phenomenon is repeated, and as a result, the SIR spectrum is recorded. It has been proven that in the range from 400 cm-1 to 4000 cm-1 ATR spectra of whole grain, endosperm, pericarp and embryo of the ZP 735 hybrid are similar to the previously obtained IR spectra of grain, which indicates the possibility of using a new method for molecular breeding technologies. The location of the bands of the SIR spectrum characterizes various forms of vibrations of the valence bonds of the functional groups of the organic molecules of the seed, which makes it possible to identify not only the presence of certain molecules, but also their conformation. It has been proved that by analyzing the amplitude of the SIR spectrum bands (maximum intensity amplitude and high intensity amplitude), it is possible to control changes in the content of a number of organic compounds in seed tissues: proteins, lipids, sugars, esters, amides, ketones, aldehydes, carboxylic acids, simple ethers, phenols, alcohols, aromatic hydrocarbons, acyclic compounds, alkenes, alkanes and alkynes. The obtained results are important for testing the presence of the necessary organic compounds in the grain or changes in their confor-mation during the selection process. The important advantages of SIR spectroscopy compared to IR spectroscopy include, on the one hand, the preservation of the nativeness of the object (the possibility of conducting research without fixing or staining the object) and simple sample preparation, on the other hand, an effective assessment of the content and conformation of molecules with high sensitivity (resolution about 1.0 cm-1). The implementation of the developed methodology for the formation of molecular breeding technology will increase the profitability of cultivation and the efficiency of breed-ing not only corn, but also other agricultural plants.
PB  - Moskva : Сельскохозяйственная биология
T2  - Сельскохозяйственная биология [Agricultural Biology]
T1  - ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.)
T1  - USE OF INTERNAL REFLECTION SPECTROSCOPY FOR MAIZE (Zea mays L.) GRAIN DIAGNOSIS
VL  - 57
IS  - 5
SP  - 933
EP  - 944
DO  - 10.15389/agrobiology.2022.5.933rus
ER  - 

@article{
author = "Radenovic, Cedomir and Maksimov, Georgy and Kuramshina, Gulnara and Shutova, Vitalina and Hao, Jingrao and Delić, Nenad and Sečanski, Mile and Popović, Aleksandar and Bajuk-Bogdanovic, Danica and Radosavljević, Milica and Pavlov, Jovan",
year = "2022",
abstract = "Инфракрасная (ИК) спектроскопия и спектроскопия комбинационного рассеяния (КР) — современные методы, на основе которых успешно формируются биотехнологические подходы, поз-воляющие проводить генетический и функциональный анализ отдельных органов и тканей растений на молекулярном уровне. В представленной работе с помощью спектроскопии поверхностного внут-реннего отражения (ПВО), которая представляет собой модификацию ИК-спектроскопии, впервые зарегистрированы и выявлены различия в содержании и конформации биомолекул в гомогенатах целого зерна гибридов кукурузы ZP 735 и его компонентов (эндосперм, перикарп, зародыш). Нашей целью была разработка методологии спектроскопии поверхностного внутреннего отражения для идентификации органических молекул, их содержания и конформации в семени кукурузы и его тканях. Объектом исследования служили зерна гибрида кукурузы (Zea mays L.) ZP 735 (оригина-тор — Maize Research Institute, Zemun Polje, г. Белград, Сербия). Методом случайной выборки было отобрано 30 зерен. Для получения ПВО-спектров образцы целых зерен и их частей (эндо-сперма, перикарпа и зародыша) измельчали, гомогенизировали и помещали в специальную ячейку прибора. Для регистрации ПВО-спектров использовали спектрометр (Nicolet™ iS20 FTIR Spectro-meter, «Thermo Scientific», США) с алмазом (diamond ATR crystal, «Thermo Scientific», США), для анализа спектров — пакет программ («Thermo Scientific», США). ПВО-спектры сопоставляли в библиотеке данных программы Advanced ATR correction Algorithm. Метод ПВО-спектроскопии ос-нован на отражении пучка света на границе раздела двух фаз: фазы кристалла с высоким показа-телем преломления, входящего в состав прибора ПВО, и фазы исследуемого образца с более низ-ким показателем преломления. В процессе измерения луч света проникает на небольшую глубину в фазу образца, где частично поглощается. При последующих облучениях образца это явление повторяется, и в результате регистрируется ПВО-спектр. Доказано, что в диапазоне от 400 см1 до 4000 см1 ПВО-спектры целого зерна, эндосперма, перикарпа и зародыша гибрида ZP 735 были аналогичны полученным ранее ИК-спектрам зерна, что свидетельствует о возможности использо-вания нового метода для технологий молекулярной селекции. Расположение полос ПВО-спектра характеризует различные формы колебаний валентных связей функциональных групп органических молекул семени, что позволяет идентифицировать не только наличие определенных молекул, но и их конформацию. Доказано, что с помощью анализа амплитуды полос ПВО-спектра (максималь-ная амплитуда интенсивности и высокая амплитуда интенсивности) в тканях семени можно кон-тролировать изменение содержания ряда органических соединений: протеинов, липидов, сахаров, сложных эфиров, амидов, кетонов, альдегидов, карбоновых кислот, простых эфиров, фенолов, спиртов, ароматических углеводородов, ациклических соединений, алкенов, алканов и алкинов. Полученные результаты важны для тестирования наличия в зерне необходимых органических со-единений или изменения их конформации в процессе селекции. К важным преимуществам метода ПВО-пектроскопии по сравнению с ИК-спектроскопией относятся, с одной стороны, сохранение нативности объекта (возможность проведения исследования без фиксации или окрашивания) и простая пробоподготовка, с другой — эффективная оценка содержания и конформации молекул с высокой чувствительностью (разрешение порядка 1,0 см1). Реализация разработанной методики для формирования технологии молекулярной селекции повысит рентабельность культивирования и эффективность селекции не только кукурузы, но и других сельскохозяйственных растений., Infrared (IR) spectroscopy and Raman spectroscopy (RS) are modern methods on the basis of which biotechnological approaches are being successfully developed that allow genetic and func-tional analysis of individual plant organs and tissues at the molecular level. In the present work, using surface internal reflection spectroscopy (SIR), which is a modification of IR spectroscopy, differences in the content and conformation of biomolecules in grain homogenates and its components (endo-sperm, pericarp, germ) of ZP 735 maize hybrids were first recorded and revealed. Our goal was to develop a methodology for surface SIR for the identification of organic molecules, their content and conformation in corn seed and its tissues. The grains of the corn hybrid (Zea mays L.) ZP 735 (origi-nator Maize Research Institute, Zemun Polje, Belgrade, Serbia) served as the object of the study. Thirty grains were selected by random sampling. To obtain SIR spectra, samples of grains and their parts (endosperm, pericarp, and embryo) were crushed, homogenized, and placed in a special cell of the device. A spectrometer (FT-IR spectrophotometer, Thermo Scientific, USA) with diamond (dia-mond ATR crystal, Thermo Scientific, USA) was used to record the SIR spectra, and a software package (Thermo Scientific™ ) was used to analyze the spectra. The SIR spectra were compared in the data library of the Advanced ATR correction Algorithm program. The SIR spectroscopy method is based on the reflection of a light beam at the interface between two phases: the phase of a crystal with a high refractive index, which is part of the SIR device, and the phase of the sample under study with a lower refractive index. During the measurement, the light beam penetrates to a small depth into the phase of the sample, where it is partially absorbed. During subsequent irradiation of the sample, this phenomenon is repeated, and as a result, the SIR spectrum is recorded. It has been proven that in the range from 400 cm-1 to 4000 cm-1 ATR spectra of whole grain, endosperm, pericarp and embryo of the ZP 735 hybrid are similar to the previously obtained IR spectra of grain, which indicates the possibility of using a new method for molecular breeding technologies. The location of the bands of the SIR spectrum characterizes various forms of vibrations of the valence bonds of the functional groups of the organic molecules of the seed, which makes it possible to identify not only the presence of certain molecules, but also their conformation. It has been proved that by analyzing the amplitude of the SIR spectrum bands (maximum intensity amplitude and high intensity amplitude), it is possible to control changes in the content of a number of organic compounds in seed tissues: proteins, lipids, sugars, esters, amides, ketones, aldehydes, carboxylic acids, simple ethers, phenols, alcohols, aromatic hydrocarbons, acyclic compounds, alkenes, alkanes and alkynes. The obtained results are important for testing the presence of the necessary organic compounds in the grain or changes in their confor-mation during the selection process. The important advantages of SIR spectroscopy compared to IR spectroscopy include, on the one hand, the preservation of the nativeness of the object (the possibility of conducting research without fixing or staining the object) and simple sample preparation, on the other hand, an effective assessment of the content and conformation of molecules with high sensitivity (resolution about 1.0 cm-1). The implementation of the developed methodology for the formation of molecular breeding technology will increase the profitability of cultivation and the efficiency of breed-ing not only corn, but also other agricultural plants.",
publisher = "Moskva : Сельскохозяйственная биология",
journal = "Сельскохозяйственная биология [Agricultural Biology]",
title = "ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.), USE OF INTERNAL REFLECTION SPECTROSCOPY FOR MAIZE (Zea mays L.) GRAIN DIAGNOSIS",
volume = "57",
number = "5",
pages = "933-944",
doi = "10.15389/agrobiology.2022.5.933rus"
}

Radenovic, C., Maksimov, G., Kuramshina, G., Shutova, V., Hao, J., Delić, N., Sečanski, M., Popović, A., Bajuk-Bogdanovic, D., Radosavljević, M.,& Pavlov, J.. (2022). ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.). in Сельскохозяйственная биология [Agricultural Biology]
Moskva : Сельскохозяйственная биология., 57(5), 933-944.
https://doi.org/10.15389/agrobiology.2022.5.933rus

Radenovic C, Maksimov G, Kuramshina G, Shutova V, Hao J, Delić N, Sečanski M, Popović A, Bajuk-Bogdanovic D, Radosavljević M, Pavlov J. ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.). in Сельскохозяйственная биология [Agricultural Biology]. 2022;57(5):933-944.
doi:10.15389/agrobiology.2022.5.933rus .

Radenovic, Cedomir, Maksimov, Georgy, Kuramshina, Gulnara, Shutova, Vitalina, Hao, Jingrao, Delić, Nenad, Sečanski, Mile, Popović, Aleksandar, Bajuk-Bogdanovic, Danica, Radosavljević, Milica, Pavlov, Jovan, "ИСПОЛЬЗОВАНИЕ МЕТОДА СПЕКТРОСКОПИИ ПОВЕРХНОСТНОГО ВНУТРЕННЕГО ОТРАЖЕНИЯ ДЛЯ ДИАГНОСТИКИ ЗЕРНА КУКУРУЗЫ (Zea mays L.)" in Сельскохозяйственная биология [Agricultural Biology], 57, no. 5 (2022):933-944,
https://doi.org/10.15389/agrobiology.2022.5.933rus . .

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Delić, Nenad
AU  - Radosavljević, Milica
AU  - Jovanović, Života
AU  - Sečanski, Mile
AU  - Popović, Aleksandar
AU  - Crevar, Miloš
AU  - Radosavljević, Nebojša
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/826
AB  - Introduction/purpose: This paper presents the results of several different research studies. The inbred lines ZPPL 146 and ZPPL 159 and the maize hybrids ZP 633, ZP 735, and ZP 737 are primarily intended for human and livestock nutrition. Their selection took about four decades. Methods: Spectral bands were registered using the method of resonant Raman spectroscopy of the leaves of inbred maize lines. These spectral bands indicate the conformational characteristics of not only carotenoid molecules but also other compounds (phosphate, gluten, and amide III) in the leaf. Results: A systematic examination of the inbred lines ZPPL 146 and ZPPL 159 and their maize hybrids ZP 633, ZP 735, and ZP 737 was performed in this paper. It was stated that the new inbred lines of corn, i.e. ZPPL 146 and ZPPL 159, are rich in carotenoids and yellow pigments. These lines also have significant quantities of other valuable bioactive compounds and good physical characteristics. The lines have an upright position of the top leaves and belong to the group of maize lines with significant characteristics of the photosynthetic model. They are resistant to high temperatures and are drought tolerant. Conclusion: This paper presents the relevant properties, characteristics and parameters of the new studied inbred maize lines that can be used in selection processes in the future. High-yielding and high-quality maize hybrids, i.e. ZP 633, ZP 735, and ZP 737, have been created from the mentioned inbred maize lines. They are recognizable by their qualities. The hybrid ZP633 is especially noteworthy for human consumption (children and the elderly). Further, from the agronomic-veterinary point of view, it is confirmed that the hybrids ZP 735 and ZP 737 are the most suitable for livestock feeding with the programmed use of corn silage. The relevant agronomic, morphological and nutritional properties of the maize hybrids ZP 633, ZP 735, and ZP 737 are also presented in this paper. The results regarding the grain structure and yield height for grain and silage for the hybrids ZP 677 and ZP 684, produced in Serbia and the countries of Southeastern Europe, are also given.
PB  - Beograd : Univerzitet odbrane u Beogradu, Vojna akademija
T2  - Vojnotehnički glasnik
T1  - High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food
VL  - 69
IS  - 1
SP  - 114
EP  - 147
DO  - 10.5937/vojteh69-29512
ER  - 

@article{
author = "Radenović, Čedomir and Delić, Nenad and Radosavljević, Milica and Jovanović, Života and Sečanski, Mile and Popović, Aleksandar and Crevar, Miloš and Radosavljević, Nebojša",
year = "2021",
abstract = "Introduction/purpose: This paper presents the results of several different research studies. The inbred lines ZPPL 146 and ZPPL 159 and the maize hybrids ZP 633, ZP 735, and ZP 737 are primarily intended for human and livestock nutrition. Their selection took about four decades. Methods: Spectral bands were registered using the method of resonant Raman spectroscopy of the leaves of inbred maize lines. These spectral bands indicate the conformational characteristics of not only carotenoid molecules but also other compounds (phosphate, gluten, and amide III) in the leaf. Results: A systematic examination of the inbred lines ZPPL 146 and ZPPL 159 and their maize hybrids ZP 633, ZP 735, and ZP 737 was performed in this paper. It was stated that the new inbred lines of corn, i.e. ZPPL 146 and ZPPL 159, are rich in carotenoids and yellow pigments. These lines also have significant quantities of other valuable bioactive compounds and good physical characteristics. The lines have an upright position of the top leaves and belong to the group of maize lines with significant characteristics of the photosynthetic model. They are resistant to high temperatures and are drought tolerant. Conclusion: This paper presents the relevant properties, characteristics and parameters of the new studied inbred maize lines that can be used in selection processes in the future. High-yielding and high-quality maize hybrids, i.e. ZP 633, ZP 735, and ZP 737, have been created from the mentioned inbred maize lines. They are recognizable by their qualities. The hybrid ZP633 is especially noteworthy for human consumption (children and the elderly). Further, from the agronomic-veterinary point of view, it is confirmed that the hybrids ZP 735 and ZP 737 are the most suitable for livestock feeding with the programmed use of corn silage. The relevant agronomic, morphological and nutritional properties of the maize hybrids ZP 633, ZP 735, and ZP 737 are also presented in this paper. The results regarding the grain structure and yield height for grain and silage for the hybrids ZP 677 and ZP 684, produced in Serbia and the countries of Southeastern Europe, are also given.",
publisher = "Beograd : Univerzitet odbrane u Beogradu, Vojna akademija",
journal = "Vojnotehnički glasnik",
title = "High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food",
volume = "69",
number = "1",
pages = "114-147",
doi = "10.5937/vojteh69-29512"
}

Radenović, Č., Delić, N., Radosavljević, M., Jovanović, Ž., Sečanski, M., Popović, A., Crevar, M.,& Radosavljević, N.. (2021). High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food. in Vojnotehnički glasnik
Beograd : Univerzitet odbrane u Beogradu, Vojna akademija., 69(1), 114-147.
https://doi.org/10.5937/vojteh69-29512

Radenović Č, Delić N, Radosavljević M, Jovanović Ž, Sečanski M, Popović A, Crevar M, Radosavljević N. High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food. in Vojnotehnički glasnik. 2021;69(1):114-147.
doi:10.5937/vojteh69-29512 .

Radenović, Čedomir, Delić, Nenad, Radosavljević, Milica, Jovanović, Života, Sečanski, Mile, Popović, Aleksandar, Crevar, Miloš, Radosavljević, Nebojša, "High-yielding and chemically enriched maize hybrids bred in Serbia - the best basis for super quality feed and food" in Vojnotehnički glasnik, 69, no. 1 (2021):114-147,
https://doi.org/10.5937/vojteh69-29512 . .

TY  - CONF
AU  - Božić, Manja
AU  - Ignjatović-Micić, Dragana
AU  - Vancetovic, Jelena
AU  - Markovic, Ksenija
AU  - Nikolic, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1143
AB  - A large number of strategies in crop production today is focussed on ensuring a decrease in yield loss occurring as a result of the increasing temperatures and drought arising during late spring and summer, which are only some of the consequences of climate change. One of those strategies is earlier sowing, during the colder months in early spring, which means that finding methods that quickly and accurately estimate low temperature tolerance of different crops, including maize, is becoming increasingly important. Our idea was to use the expression of Zea mays sp. cold induced genes (ZmCOI) for this purpose, and to see if there is a significant correlation between the quantities of certain ZmCOI mRNA and low temperature tolerance. This was done through an experiment that included growing several maize lines known from breeders experience to be sensitive or tolerant to cold, under optimal and low temperatures, 22° C and 6° C, respectively. Samples (plant leaves) were taken during the V4 growth stage, at different time points during the treatment (6h and 24h) and used for RNA extraction, cDNA synthesis and qPCR expression analysis for the selected genes (ZmDREB2A, ZmERF3, ZmACA1). Preliminary results showed changes in the expression of these genes between chosen sensitive and tolerant inbred lines. These results provide a good basis for further studies with more cold induced genes and inbred lines that will hopefully confirm their effectiveness as a tool for determination of tolerance to low temperatures.
PB  - East Sarajevo : Faculty of agriculture
C3  - 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts
T1  - Expression analysis of cold induced genes in early developmental stages of maize
UR  - https://hdl.handle.net/21.15107/rcub_rik_1143
ER  - 

@conference{
author = "Božić, Manja and Ignjatović-Micić, Dragana and Vancetovic, Jelena and Markovic, Ksenija and Nikolic, Ana",
year = "2021",
abstract = "A large number of strategies in crop production today is focussed on ensuring a decrease in yield loss occurring as a result of the increasing temperatures and drought arising during late spring and summer, which are only some of the consequences of climate change. One of those strategies is earlier sowing, during the colder months in early spring, which means that finding methods that quickly and accurately estimate low temperature tolerance of different crops, including maize, is becoming increasingly important. Our idea was to use the expression of Zea mays sp. cold induced genes (ZmCOI) for this purpose, and to see if there is a significant correlation between the quantities of certain ZmCOI mRNA and low temperature tolerance. This was done through an experiment that included growing several maize lines known from breeders experience to be sensitive or tolerant to cold, under optimal and low temperatures, 22° C and 6° C, respectively. Samples (plant leaves) were taken during the V4 growth stage, at different time points during the treatment (6h and 24h) and used for RNA extraction, cDNA synthesis and qPCR expression analysis for the selected genes (ZmDREB2A, ZmERF3, ZmACA1). Preliminary results showed changes in the expression of these genes between chosen sensitive and tolerant inbred lines. These results provide a good basis for further studies with more cold induced genes and inbred lines that will hopefully confirm their effectiveness as a tool for determination of tolerance to low temperatures.",
publisher = "East Sarajevo : Faculty of agriculture",
journal = "12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts",
title = "Expression analysis of cold induced genes in early developmental stages of maize",
url = "https://hdl.handle.net/21.15107/rcub_rik_1143"
}

Božić, M., Ignjatović-Micić, D., Vancetovic, J., Markovic, K.,& Nikolic, A.. (2021). Expression analysis of cold induced genes in early developmental stages of maize. in 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts
East Sarajevo : Faculty of agriculture..
https://hdl.handle.net/21.15107/rcub_rik_1143

Božić M, Ignjatović-Micić D, Vancetovic J, Markovic K, Nikolic A. Expression analysis of cold induced genes in early developmental stages of maize. in 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1143 .

Božić, Manja, Ignjatović-Micić, Dragana, Vancetovic, Jelena, Markovic, Ksenija, Nikolic, Ana, "Expression analysis of cold induced genes in early developmental stages of maize" in 12. International scientific agriculture symposium AGROSYM 2021., Jahorina, B i H, 07.10.2021. - Book of abstracts (2021),
https://hdl.handle.net/21.15107/rcub_rik_1143 .

TY  - JOUR
AU  - Banović Đeri, Bojana
AU  - Božić, Manja
AU  - Dudić, Dragana
AU  - Vićić, Ivan
AU  - Milivojević, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Samardžić, Jelena
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1137
AB  - One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes—chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.
PB  - Hoboken, New Jersey : Wiley
T2  - Journal of Agronomy and Crop Science
T1  - Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes
VL  - 208
SP  - 12529
DO  - 10.1111/jac.12529
ER  - 

@article{
author = "Banović Đeri, Bojana and Božić, Manja and Dudić, Dragana and Vićić, Ivan and Milivojević, Marija and Ignjatović-Micić, Dragana and Samardžić, Jelena and Vančetović, Jelena and Delić, Nenad and Nikolić, Ana",
year = "2021",
abstract = "One of the strategies for overcoming global climate change threatening to decrease maize yield is early sowing. To contribute to the development of cold-tolerant hybrids this research focused on the genetic background's comparative analysis in maize inbreds with good combining ability. Leaf whole-transcriptome sequencing of 46 maize genotypes revealed 77 differentially expressed genes (DEGs) between Lancaster and other heterotic groups (i.e. BSSS, Iowa dent, Ohio), referred to as non-Lancaster group, under optimal growing conditions. Cold test of the subset of four Lancaster and four non-Lancaster lines showed that the former were cold sensitive and the latter cold tolerant. Cold-induced expression analysis of seven DEGs in eight lines revealed different expression regulation dependent on the duration of cold exposure and genetic background for six out of seven analysed genes—chloroplast ATP-sulphurylase, photosystem II cytochrome b559 alpha subunit, CIPK serine-threonine protein kinase 15, glutamyl-tRNA reductase, photosystem II reaction centre protein I and Calvin cycle CP12-chloroplastic-like encoding genes. The results imply that differently regulated basic processes between Lancaster and non-Lancaster maize group involve, at least, photosynthesis and sulphate assimilation, contributing to their different cold response and different adaptation to low temperatures.",
publisher = "Hoboken, New Jersey : Wiley",
journal = "Journal of Agronomy and Crop Science",
title = "Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes",
volume = "208",
pages = "12529",
doi = "10.1111/jac.12529"
}

Banović Đeri, B., Božić, M., Dudić, D., Vićić, I., Milivojević, M., Ignjatović-Micić, D., Samardžić, J., Vančetović, J., Delić, N.,& Nikolić, A.. (2021). Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes. in Journal of Agronomy and Crop Science
Hoboken, New Jersey : Wiley., 208, 12529.
https://doi.org/10.1111/jac.12529

Banović Đeri B, Božić M, Dudić D, Vićić I, Milivojević M, Ignjatović-Micić D, Samardžić J, Vančetović J, Delić N, Nikolić A. Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes. in Journal of Agronomy and Crop Science. 2021;208:12529.
doi:10.1111/jac.12529 .

Banović Đeri, Bojana, Božić, Manja, Dudić, Dragana, Vićić, Ivan, Milivojević, Marija, Ignjatović-Micić, Dragana, Samardžić, Jelena, Vančetović, Jelena, Delić, Nenad, Nikolić, Ana, "Leaf transcriptome analysis of Lancaster versus other heterotic groups’ maize inbred lines revealed different regulation of cold‐responsive genes" in Journal of Agronomy and Crop Science, 208 (2021):12529,
https://doi.org/10.1111/jac.12529 . .

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Božinović, Sofija
AU  - Vančetović, Jelena
AU  - Radosavljević, Milica
AU  - Žilić, Slađana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/843
AB  - Physical traits, kernel structure and chemical composition of five yellow and five red kernel maize hybrids were the subjects of this study. The 1000-kernel weight, a physical indicator of grain quality, ranged from 325.76±7.47 g (ZP 555 red) to 375.63±4.18 g (ZP 606). The
hard endosperm fraction content varied between 57.66±0.93% (ZP 4007) and 67.08±0.42% (ZP
366 red). Regarding chemical composition, starch was predominant constituent ranging from
66.80±0.18 (ZP 4007 red) to 72.96±0.37 (ZP 606). The highest protein content was detected in
ZP 606 (10.72±0.11) and the lowest in ZP 7007 red hybrid (8.63±0.04%). Milling response was
highly influenced by hard endosperm fraction content (0.81**), and starch content was strongly
correlated to 1000-kernel weight (0.77**). Whole-grain maize flours produced from yellow and
red kernels without removing the germ are naturally gluten-free and can be used as functional
food ingredients. All yellow and red kernel maize hybrids investigated in this study showed good
quality parameters regarding physical properties and variations in chemical composition which
makes them suitable for different industrial uses, primarily for food and feed production.
AB  - Fizičke karakteristike, struktura zrna i hemijski sastav pet hibrida kukuruza žutog i pet crvenog zrna, bili su predmet ovog istraživanja. Masa 1000 zrna, važan fizički pokazatelj kvaliteta
zrna, kretala se u rasponu od 325,76±7,47 g (ZP 555 crveni) do 375,63±4,18 g (ZP 606). Sadržaj
tvrde frakcije endosperma bio je od 57,66±0,93% (ZP 4007) do 67,08±0,42% (ZP 366 crveni), a
meke od 32,92 ± 0,43% (ZP 366 crveni) do 42,34 ± 0,93% (ZP 4007). Skrob, kao najzastupljenija hemijska komponenta, varirao je od 66,80±0,18 (ZP 4007 crveni) do 72,96±0,37 (ZP 606).
Najviši udeo proteina određen je u zrnu hibrida ZP 606 (10,72±0,11%), a najniži u ZP 7007
(8,63 ± 0,04%). Otpornost na mlevenje bila je u visokoj pozitivnoj korelaciji sa udelom tvrdog
endosperma (0.81**), a sadržaj skroba sa hektolitarskom masom (0,77**). Integralna kukuruzna
brašna, dobijena mlevenjem celog zrna žutih i crvenih hibrida, su prirodno bezglutenska i mogu
se koristiti kao sastojci funkcionalne hrane. Svi hibridi kukuruza žutog i crvenog zrna ispitivani u
ovom istraživanju pokazali su dobre parametre kvaliteta u pogledu fizičkih svojstava i varijacija u
hemijskom sastavu što ih čini pogodnim za različite industrijske namene, prvenstveno za proizvodnju hrane za ljude i životinje.
PB  - Belgrade : Serbian Association of Plant Breedings and Seed Producers
PB  - Beograd : Društvo selekcionara i semenara Republike Srbije
T2  - Selekcija i semenarstvo
T1  - Grain properties of yellow and red kernel maize hybrids from Serbia
T1  - Fizičko-hemijska svojstva zrna žutih i crvenih hibrida kukuruza iz Srbije
VL  - 26
IS  - 2
SP  - 7
EP  - 14
DO  - 10.5937/SelSem2002007N
ER  - 

@article{
author = "Nikolić, Valentina and Božinović, Sofija and Vančetović, Jelena and Radosavljević, Milica and Žilić, Slađana",
year = "2021",
abstract = "Physical traits, kernel structure and chemical composition of five yellow and five red kernel maize hybrids were the subjects of this study. The 1000-kernel weight, a physical indicator of grain quality, ranged from 325.76±7.47 g (ZP 555 red) to 375.63±4.18 g (ZP 606). The
hard endosperm fraction content varied between 57.66±0.93% (ZP 4007) and 67.08±0.42% (ZP
366 red). Regarding chemical composition, starch was predominant constituent ranging from
66.80±0.18 (ZP 4007 red) to 72.96±0.37 (ZP 606). The highest protein content was detected in
ZP 606 (10.72±0.11) and the lowest in ZP 7007 red hybrid (8.63±0.04%). Milling response was
highly influenced by hard endosperm fraction content (0.81**), and starch content was strongly
correlated to 1000-kernel weight (0.77**). Whole-grain maize flours produced from yellow and
red kernels without removing the germ are naturally gluten-free and can be used as functional
food ingredients. All yellow and red kernel maize hybrids investigated in this study showed good
quality parameters regarding physical properties and variations in chemical composition which
makes them suitable for different industrial uses, primarily for food and feed production., Fizičke karakteristike, struktura zrna i hemijski sastav pet hibrida kukuruza žutog i pet crvenog zrna, bili su predmet ovog istraživanja. Masa 1000 zrna, važan fizički pokazatelj kvaliteta
zrna, kretala se u rasponu od 325,76±7,47 g (ZP 555 crveni) do 375,63±4,18 g (ZP 606). Sadržaj
tvrde frakcije endosperma bio je od 57,66±0,93% (ZP 4007) do 67,08±0,42% (ZP 366 crveni), a
meke od 32,92 ± 0,43% (ZP 366 crveni) do 42,34 ± 0,93% (ZP 4007). Skrob, kao najzastupljenija hemijska komponenta, varirao je od 66,80±0,18 (ZP 4007 crveni) do 72,96±0,37 (ZP 606).
Najviši udeo proteina određen je u zrnu hibrida ZP 606 (10,72±0,11%), a najniži u ZP 7007
(8,63 ± 0,04%). Otpornost na mlevenje bila je u visokoj pozitivnoj korelaciji sa udelom tvrdog
endosperma (0.81**), a sadržaj skroba sa hektolitarskom masom (0,77**). Integralna kukuruzna
brašna, dobijena mlevenjem celog zrna žutih i crvenih hibrida, su prirodno bezglutenska i mogu
se koristiti kao sastojci funkcionalne hrane. Svi hibridi kukuruza žutog i crvenog zrna ispitivani u
ovom istraživanju pokazali su dobre parametre kvaliteta u pogledu fizičkih svojstava i varijacija u
hemijskom sastavu što ih čini pogodnim za različite industrijske namene, prvenstveno za proizvodnju hrane za ljude i životinje.",
publisher = "Belgrade : Serbian Association of Plant Breedings and Seed Producers, Beograd : Društvo selekcionara i semenara Republike Srbije",
journal = "Selekcija i semenarstvo",
title = "Grain properties of yellow and red kernel maize hybrids from Serbia, Fizičko-hemijska svojstva zrna žutih i crvenih hibrida kukuruza iz Srbije",
volume = "26",
number = "2",
pages = "7-14",
doi = "10.5937/SelSem2002007N"
}

Nikolić, V., Božinović, S., Vančetović, J., Radosavljević, M.,& Žilić, S.. (2021). Grain properties of yellow and red kernel maize hybrids from Serbia. in Selekcija i semenarstvo
Belgrade : Serbian Association of Plant Breedings and Seed Producers., 26(2), 7-14.
https://doi.org/10.5937/SelSem2002007N

Nikolić V, Božinović S, Vančetović J, Radosavljević M, Žilić S. Grain properties of yellow and red kernel maize hybrids from Serbia. in Selekcija i semenarstvo. 2021;26(2):7-14.
doi:10.5937/SelSem2002007N .

Nikolić, Valentina, Božinović, Sofija, Vančetović, Jelena, Radosavljević, Milica, Žilić, Slađana, "Grain properties of yellow and red kernel maize hybrids from Serbia" in Selekcija i semenarstvo, 26, no. 2 (2021):7-14,
https://doi.org/10.5937/SelSem2002007N . .

TY  - JOUR
AU  - Anđelković, Violeta
AU  - Cvejić, Sandra
AU  - Jocić, Siniša
AU  - Kondić-Špika, Ankica
AU  - Marjanović Jeromela, Ana
AU  - Mikić, Sanja
AU  - Prodanović, Slaven
AU  - Radanović, Aleksandra
AU  - Savić-Ivanov, Milena
AU  - Trkulja, Dragana
AU  - Miladinović, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/815
AB  - Plant genetic resources are a link between agriculture, environment and trade, so their conservation requires cooperation from different sectors. The existing diversity in genetic resources is the foundation in breeding for new challenges or new markets in the future. The number of crop genetic resources in Serbia is obscure because there is no national inventory. It is thought that there are about 15,000 seed accessions and 3500 accessions of fruit trees and grape in collections of different national institutes and stakeholders. In the National Plant Gene Bank, there are more than 4000 accessions of nearly 250 plant species. Crops kept in ex situ collections are used in breeding programs and interspecific crosses, for selection and introduction of desired traits through pre-breeding programs. Serbia as a state participates in all national and international efforts for preservation, management and use of plant genetic resources. Additionally, Serbia has also established a set of national regulations related to plant genetic resources and their use in breeding. Since Serbia is one of Europe’s most important crop producers, especially in maize (11% of EU-27 production), soya (35%), sunflowers (6%) and sugar beet (2.5%), this paper discusses certain issues and achievements in the use of plant genetic resources in cereal and oil crops improvement in Serbia, as well as national and international regulations affecting their exploitation.
PB  - Springer Science+Business Media B.V.
T2  - Genetic Resources and Crop Evolution
T1  - Use of plant genetic resources in crop improvement–example of Serbia
DO  - 10.1007/s10722-020-01029-9
ER  - 

@article{
author = "Anđelković, Violeta and Cvejić, Sandra and Jocić, Siniša and Kondić-Špika, Ankica and Marjanović Jeromela, Ana and Mikić, Sanja and Prodanović, Slaven and Radanović, Aleksandra and Savić-Ivanov, Milena and Trkulja, Dragana and Miladinović, Dragana",
year = "2020",
abstract = "Plant genetic resources are a link between agriculture, environment and trade, so their conservation requires cooperation from different sectors. The existing diversity in genetic resources is the foundation in breeding for new challenges or new markets in the future. The number of crop genetic resources in Serbia is obscure because there is no national inventory. It is thought that there are about 15,000 seed accessions and 3500 accessions of fruit trees and grape in collections of different national institutes and stakeholders. In the National Plant Gene Bank, there are more than 4000 accessions of nearly 250 plant species. Crops kept in ex situ collections are used in breeding programs and interspecific crosses, for selection and introduction of desired traits through pre-breeding programs. Serbia as a state participates in all national and international efforts for preservation, management and use of plant genetic resources. Additionally, Serbia has also established a set of national regulations related to plant genetic resources and their use in breeding. Since Serbia is one of Europe’s most important crop producers, especially in maize (11% of EU-27 production), soya (35%), sunflowers (6%) and sugar beet (2.5%), this paper discusses certain issues and achievements in the use of plant genetic resources in cereal and oil crops improvement in Serbia, as well as national and international regulations affecting their exploitation.",
publisher = "Springer Science+Business Media B.V.",
journal = "Genetic Resources and Crop Evolution",
title = "Use of plant genetic resources in crop improvement–example of Serbia",
doi = "10.1007/s10722-020-01029-9"
}

Anđelković, V., Cvejić, S., Jocić, S., Kondić-Špika, A., Marjanović Jeromela, A., Mikić, S., Prodanović, S., Radanović, A., Savić-Ivanov, M., Trkulja, D.,& Miladinović, D.. (2020). Use of plant genetic resources in crop improvement–example of Serbia. in Genetic Resources and Crop Evolution
Springer Science+Business Media B.V...
https://doi.org/10.1007/s10722-020-01029-9

Anđelković V, Cvejić S, Jocić S, Kondić-Špika A, Marjanović Jeromela A, Mikić S, Prodanović S, Radanović A, Savić-Ivanov M, Trkulja D, Miladinović D. Use of plant genetic resources in crop improvement–example of Serbia. in Genetic Resources and Crop Evolution. 2020;.
doi:10.1007/s10722-020-01029-9 .

Anđelković, Violeta, Cvejić, Sandra, Jocić, Siniša, Kondić-Špika, Ankica, Marjanović Jeromela, Ana, Mikić, Sanja, Prodanović, Slaven, Radanović, Aleksandra, Savić-Ivanov, Milena, Trkulja, Dragana, Miladinović, Dragana, "Use of plant genetic resources in crop improvement–example of Serbia" in Genetic Resources and Crop Evolution (2020),
https://doi.org/10.1007/s10722-020-01029-9 . .

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Vukadinović, Jelena
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/791
AB  - Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins.
AB  - Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika
T1  - Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions
T1  - Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena
VL  - 52
IS  - 1
SP  - 273
EP  - 289
DO  - 10.2298/GENSR2001273V
ER  - 

@article{
author = "Vančetović, Jelena and Kostadinović, Marija and Božinović, Sofija and Nikolić, Ana and Vukadinović, Jelena and Marković, Ksenija and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins., Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika",
title = "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions, Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena",
volume = "52",
number = "1",
pages = "273-289",
doi = "10.2298/GENSR2001273V"
}

Vančetović, J., Kostadinović, M., Božinović, S., Nikolić, A., Vukadinović, J., Marković, K.,& Ignjatović-Micić, D.. (2020). Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika
Beograd : Društvo genetičara Srbije., 52(1), 273-289.
https://doi.org/10.2298/GENSR2001273V

Vančetović J, Kostadinović M, Božinović S, Nikolić A, Vukadinović J, Marković K, Ignjatović-Micić D. Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika. 2020;52(1):273-289.
doi:10.2298/GENSR2001273V .

Vančetović, Jelena, Kostadinović, Marija, Božinović, Sofija, Nikolić, Ana, Vukadinović, Jelena, Marković, Ksenija, Ignjatović-Micić, Dragana, "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions" in Genetika, 52, no. 1 (2020):273-289,
https://doi.org/10.2298/GENSR2001273V . .

TY  - JOUR
AU  - Popović, Aleksandar
AU  - Kravić, Natalija
AU  - Babić, Milosav
AU  - Prodanović, Slaven
AU  - Sečanski, Mile
AU  - Babić, Vojka
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/797
AB  - Significant amounts of crop diversity, especially in out-crossing species like maize (Zea  mays  L.)  is,  are  often  distributed both between and within related groups of accessions. Thus, a detailed characterization and classification of gene bank accessions should be performed prior to use of the best of them for introgression programs to enlarge the genetic base of the elite germplasm pool. Based on performances per se, 40 landraces were chosen out of the wide pool of Western Balkan landraces, previously clustered into 11 homogenous groups. The objective of this study  was  to  evaluate  the  heterotic  pattern  of  landraces  in  testcrossing  with  three  divergent  elite  testers  L217,  L73B013 and L255/75-5. According to the results of a two-year trial conducted at four locations, landraces 1267, 1346, 197, 1569, 1509 and 2036 expressed the best general combining abilities (GCA) for grain yield. Landraces 1960, 642, 2006, 1945, 1346, 1569, 1450, 1534, 1509 and 1665 performed well in crosses to L217, while landraces 1960, 773, 1798, 1665, 632, 877 and 1569 gave the best results in crosses to inbred tester L73B013. In crosses to inbred tester L255/75-5, the highest heterotic effects were expressed by landraces 467, 773, 1346, 1534, 2249 and 288. Particular attention is to be paid to landraces 1346, 1569 and 1509, having simultaneously high GCA and specific combining abilities (SCA). A certain number of landraces expressed heterosis with two inbred testers, indicating existence of a new unrelated heterotic pattern within the local germplasm pool evaluated.
AB  - Didelės dalies augalų, ypač kryžmadulkių, pavyzdžiui, paprastojo kukurūzo (Zea  mays L.), giminingų genotipų grupėse ir tų grupių viduje pastebima didelė įvairovė. Siekiant praplėsti genetinę bazę, prieš panaudojant selekcinę medžiagą veislių kūrimo programoms, būtina atlikti išsamų Genų banko genotipų įvertinimą ir klasifikaciją. Tyrimui buvo pasirinkta 40 vietinių veislių iš didelio Vakarų Balkanų fondo, kurios buvo sugrupuotos į 11 homogeniškų grupių.  Tyrimo  tikslas  –  įvertinti  heterozinį  vietinių  veislių  efektyvumą,  jas  kryžminant  su  trimis  skirtingais elitiniais testeriais L217, L73B013 ir L255/75-5. Pagal dvejų metų tyrimų, atliktų keturiose vietovėse, rezultatus, vietinės veislės 1267, 1346, 197, 1569, 1509 ir 2036 parodė geriausią bendrą kombinacinę gebą jas vertinant pagal grūdų derlių. Vietinės veislės 1960, 642, 2006, 1945, 1346, 1569, 1450, 1534, 1509 ir 1665 pademonstravo gerus rezultatus kryžminant su testeriu L217, o vietinės veislės 1960, 773, 1798, 1665, 632, 877 ir 1569 parodė geriausius rezultatus jas kryžminant su inbrediniu testeriu L73B013. Kryžminant su inbrediniu testeriu L255/75-5 pasireiškė didžiausias heterozinis vietinių veislių 467, 773, 1346, 1534, 2249 ir 288 efektyvumas. Didžiausią dėmesį reikėtų skirti vietinių populiacijų veislėms 1346, 1569 ir 1509, pasižyminčioms ir didele bendra, ir specifine kombinacine geba. Kai kurios vietinės veislės parodė heterozę su dviem testeriais, o tai rodo naują, nesusijusį, negiminingą tirtos vietinės genetinės medžiagos vidinį heterozinį modelį.
PB  - Vilnius : Lithuanian Research Centre for Agriculture and Forestry
PB  - Kaunas : Vytautas Magnus University
T2  - Zemdirbyste-Agriculture
T1  - Breeding potential of maize landraces evaluated by their testcross performance
VL  - 107
IS  - 2
SP  - 153
EP  - 160
DO  - 10.13080/z-a.2020.107.020
ER  - 

@article{
author = "Popović, Aleksandar and Kravić, Natalija and Babić, Milosav and Prodanović, Slaven and Sečanski, Mile and Babić, Vojka",
year = "2020",
abstract = "Significant amounts of crop diversity, especially in out-crossing species like maize (Zea  mays  L.)  is,  are  often  distributed both between and within related groups of accessions. Thus, a detailed characterization and classification of gene bank accessions should be performed prior to use of the best of them for introgression programs to enlarge the genetic base of the elite germplasm pool. Based on performances per se, 40 landraces were chosen out of the wide pool of Western Balkan landraces, previously clustered into 11 homogenous groups. The objective of this study  was  to  evaluate  the  heterotic  pattern  of  landraces  in  testcrossing  with  three  divergent  elite  testers  L217,  L73B013 and L255/75-5. According to the results of a two-year trial conducted at four locations, landraces 1267, 1346, 197, 1569, 1509 and 2036 expressed the best general combining abilities (GCA) for grain yield. Landraces 1960, 642, 2006, 1945, 1346, 1569, 1450, 1534, 1509 and 1665 performed well in crosses to L217, while landraces 1960, 773, 1798, 1665, 632, 877 and 1569 gave the best results in crosses to inbred tester L73B013. In crosses to inbred tester L255/75-5, the highest heterotic effects were expressed by landraces 467, 773, 1346, 1534, 2249 and 288. Particular attention is to be paid to landraces 1346, 1569 and 1509, having simultaneously high GCA and specific combining abilities (SCA). A certain number of landraces expressed heterosis with two inbred testers, indicating existence of a new unrelated heterotic pattern within the local germplasm pool evaluated., Didelės dalies augalų, ypač kryžmadulkių, pavyzdžiui, paprastojo kukurūzo (Zea  mays L.), giminingų genotipų grupėse ir tų grupių viduje pastebima didelė įvairovė. Siekiant praplėsti genetinę bazę, prieš panaudojant selekcinę medžiagą veislių kūrimo programoms, būtina atlikti išsamų Genų banko genotipų įvertinimą ir klasifikaciją. Tyrimui buvo pasirinkta 40 vietinių veislių iš didelio Vakarų Balkanų fondo, kurios buvo sugrupuotos į 11 homogeniškų grupių.  Tyrimo  tikslas  –  įvertinti  heterozinį  vietinių  veislių  efektyvumą,  jas  kryžminant  su  trimis  skirtingais elitiniais testeriais L217, L73B013 ir L255/75-5. Pagal dvejų metų tyrimų, atliktų keturiose vietovėse, rezultatus, vietinės veislės 1267, 1346, 197, 1569, 1509 ir 2036 parodė geriausią bendrą kombinacinę gebą jas vertinant pagal grūdų derlių. Vietinės veislės 1960, 642, 2006, 1945, 1346, 1569, 1450, 1534, 1509 ir 1665 pademonstravo gerus rezultatus kryžminant su testeriu L217, o vietinės veislės 1960, 773, 1798, 1665, 632, 877 ir 1569 parodė geriausius rezultatus jas kryžminant su inbrediniu testeriu L73B013. Kryžminant su inbrediniu testeriu L255/75-5 pasireiškė didžiausias heterozinis vietinių veislių 467, 773, 1346, 1534, 2249 ir 288 efektyvumas. Didžiausią dėmesį reikėtų skirti vietinių populiacijų veislėms 1346, 1569 ir 1509, pasižyminčioms ir didele bendra, ir specifine kombinacine geba. Kai kurios vietinės veislės parodė heterozę su dviem testeriais, o tai rodo naują, nesusijusį, negiminingą tirtos vietinės genetinės medžiagos vidinį heterozinį modelį.",
publisher = "Vilnius : Lithuanian Research Centre for Agriculture and Forestry, Kaunas : Vytautas Magnus University",
journal = "Zemdirbyste-Agriculture",
title = "Breeding potential of maize landraces evaluated by their testcross performance",
volume = "107",
number = "2",
pages = "153-160",
doi = "10.13080/z-a.2020.107.020"
}

Popović, A., Kravić, N., Babić, M., Prodanović, S., Sečanski, M.,& Babić, V.. (2020). Breeding potential of maize landraces evaluated by their testcross performance. in Zemdirbyste-Agriculture
Vilnius : Lithuanian Research Centre for Agriculture and Forestry., 107(2), 153-160.
https://doi.org/10.13080/z-a.2020.107.020

Popović A, Kravić N, Babić M, Prodanović S, Sečanski M, Babić V. Breeding potential of maize landraces evaluated by their testcross performance. in Zemdirbyste-Agriculture. 2020;107(2):153-160.
doi:10.13080/z-a.2020.107.020 .

Popović, Aleksandar, Kravić, Natalija, Babić, Milosav, Prodanović, Slaven, Sečanski, Mile, Babić, Vojka, "Breeding potential of maize landraces evaluated by their testcross performance" in Zemdirbyste-Agriculture, 107, no. 2 (2020):153-160,
https://doi.org/10.13080/z-a.2020.107.020 . .

TY  - JOUR
AU  - Popović, Aleksandar
AU  - Kravić, Natalija
AU  - Prodanović, Slaven
AU  - Filipović, Milomir
AU  - Sečanski, Mile
AU  - Babić, Vojka
AU  - Miriţescu, Mihai
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/776
AB  - The narrow genetic base of commercial maize varieties emphasise the necessity for conservation, characterisation and utilisation of germplasm stored within gene banks. Broad genetic variability preserved in the Maize Research Institute Zemun Polje (MRIZP) gene bank, which includes accessions which originated from the Western Balkan, as a part of European corn-belt, is an exceptional source of desirable traits for enriching breeders' working collections for maize breeding under temperate conditions. Preliminary screening for abiotic stress tolerance, which marked 321 maize landraces, served as the first step in stratification process of selecting a smaller number of accessions from the entire gene bank local collection. After classification of these landraces into eleven homogenous groups, the objective of this study was to continue the stratification process of selection (as a second step), based on evaluation of agro-morphological traits of interest for breeding. The conducted evaluation highlighted 40 landraces with the best per se performances, important for breeding. Out of them, 28 early-maturing flint landraces with stiff stalks, low positioned ears, high yield potential and good general ear assessment could be considered as valuable source for their introgression into elite flint germplasm pool.
PB  - Fundulea, National Agricultural Research and Development Institute
T2  - Romanian Agricultural Research
T1  - Characterisation and evaluation towards selection of maize landraces with the best per se performances
VL  - 2020
IS  - 37
SP  - 49
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_rik_776
ER  - 

@article{
author = "Popović, Aleksandar and Kravić, Natalija and Prodanović, Slaven and Filipović, Milomir and Sečanski, Mile and Babić, Vojka and Miriţescu, Mihai",
year = "2020",
abstract = "The narrow genetic base of commercial maize varieties emphasise the necessity for conservation, characterisation and utilisation of germplasm stored within gene banks. Broad genetic variability preserved in the Maize Research Institute Zemun Polje (MRIZP) gene bank, which includes accessions which originated from the Western Balkan, as a part of European corn-belt, is an exceptional source of desirable traits for enriching breeders' working collections for maize breeding under temperate conditions. Preliminary screening for abiotic stress tolerance, which marked 321 maize landraces, served as the first step in stratification process of selecting a smaller number of accessions from the entire gene bank local collection. After classification of these landraces into eleven homogenous groups, the objective of this study was to continue the stratification process of selection (as a second step), based on evaluation of agro-morphological traits of interest for breeding. The conducted evaluation highlighted 40 landraces with the best per se performances, important for breeding. Out of them, 28 early-maturing flint landraces with stiff stalks, low positioned ears, high yield potential and good general ear assessment could be considered as valuable source for their introgression into elite flint germplasm pool.",
publisher = "Fundulea, National Agricultural Research and Development Institute",
journal = "Romanian Agricultural Research",
title = "Characterisation and evaluation towards selection of maize landraces with the best per se performances",
volume = "2020",
number = "37",
pages = "49-58",
url = "https://hdl.handle.net/21.15107/rcub_rik_776"
}

Popović, A., Kravić, N., Prodanović, S., Filipović, M., Sečanski, M., Babić, V.,& Miriţescu, M.. (2020). Characterisation and evaluation towards selection of maize landraces with the best per se performances. in Romanian Agricultural Research
Fundulea, National Agricultural Research and Development Institute., 2020(37), 49-58.
https://hdl.handle.net/21.15107/rcub_rik_776

Popović A, Kravić N, Prodanović S, Filipović M, Sečanski M, Babić V, Miriţescu M. Characterisation and evaluation towards selection of maize landraces with the best per se performances. in Romanian Agricultural Research. 2020;2020(37):49-58.
https://hdl.handle.net/21.15107/rcub_rik_776 .

Popović, Aleksandar, Kravić, Natalija, Prodanović, Slaven, Filipović, Milomir, Sečanski, Mile, Babić, Vojka, Miriţescu, Mihai, "Characterisation and evaluation towards selection of maize landraces with the best per se performances" in Romanian Agricultural Research, 2020, no. 37 (2020):49-58,
https://hdl.handle.net/21.15107/rcub_rik_776 .

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Stanković, Goran
AU  - Delić, Nenad
AU  - Vančetović, Jelena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/819
AB  - Quality protein maize (QPM) (Zea mays L.) is primarily used for food in countries of tropical and sub-tropical regions
where maize is the main source of protein. Although its cultivation in temperate regions is hampered by residues of
exotic germplasm, it could be beneficial for use in livestock feeds as it was shown that substitution of standard maize
with QPM can improve livestock characteristics and decrease dietary lysine supplementation. The aim of this study
was to test 11 QPM hybrids obtained by crossing adapted QPM inbred lines for their performance in field trials in 2
yr at four locations, as well as to determine their relevant biochemical components. The main reason for rejecting nine
QPM hybrids was low grain yield, standard hybrids had higher yields on average for 37.8%. Hybrid ZPQPM6 had good
agronomic characteristics, but its biochemical components were nonsignificantly different from the standard hybrid.
Only hybrid ZPQPM13 met necessary criteria, grain yield comparable with standard hybrids, high tryptophan content
in different environments (average 0.083%) and hard endosperm (average score 1.87). Lysine content, measured after
mercantile production, was 0.44%. Quality index, although below the QPM threshold (which is 0.80%) was significantly
higher (p < 0.05) in ZPQPM13 in comparison with standard hybrid, indicating improved nutritional quality of the protein.
The results indicated that presence of exotic germplasm in these QPM hybrids is a consequential difficulty and that in their
parental inbred lines at least one more backcross with temperate germplasm should be done to select better adapted QPM.
PB  - Chile : Instituto de Investigaciones Agropecuarias, INIA
T2  - Chilean Journal of Agricultural Research
T1  - Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality
VL  - 80
IS  - 4
SP  - 598
EP  - 607
DO  - 10.4067/S0718-58392020000400598
ER  - 

@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Božinović, Sofija and Đorđević-Melnik, Olivera and Stanković, Goran and Delić, Nenad and Vančetović, Jelena",
year = "2020",
abstract = "Quality protein maize (QPM) (Zea mays L.) is primarily used for food in countries of tropical and sub-tropical regions
where maize is the main source of protein. Although its cultivation in temperate regions is hampered by residues of
exotic germplasm, it could be beneficial for use in livestock feeds as it was shown that substitution of standard maize
with QPM can improve livestock characteristics and decrease dietary lysine supplementation. The aim of this study
was to test 11 QPM hybrids obtained by crossing adapted QPM inbred lines for their performance in field trials in 2
yr at four locations, as well as to determine their relevant biochemical components. The main reason for rejecting nine
QPM hybrids was low grain yield, standard hybrids had higher yields on average for 37.8%. Hybrid ZPQPM6 had good
agronomic characteristics, but its biochemical components were nonsignificantly different from the standard hybrid.
Only hybrid ZPQPM13 met necessary criteria, grain yield comparable with standard hybrids, high tryptophan content
in different environments (average 0.083%) and hard endosperm (average score 1.87). Lysine content, measured after
mercantile production, was 0.44%. Quality index, although below the QPM threshold (which is 0.80%) was significantly
higher (p < 0.05) in ZPQPM13 in comparison with standard hybrid, indicating improved nutritional quality of the protein.
The results indicated that presence of exotic germplasm in these QPM hybrids is a consequential difficulty and that in their
parental inbred lines at least one more backcross with temperate germplasm should be done to select better adapted QPM.",
publisher = "Chile : Instituto de Investigaciones Agropecuarias, INIA",
journal = "Chilean Journal of Agricultural Research",
title = "Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality",
volume = "80",
number = "4",
pages = "598-607",
doi = "10.4067/S0718-58392020000400598"
}

Ignjatović-Micić, D., Kostadinović, M., Božinović, S., Đorđević-Melnik, O., Stanković, G., Delić, N.,& Vančetović, J.. (2020). Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality. in Chilean Journal of Agricultural Research
Chile : Instituto de Investigaciones Agropecuarias, INIA., 80(4), 598-607.
https://doi.org/10.4067/S0718-58392020000400598

Ignjatović-Micić D, Kostadinović M, Božinović S, Đorđević-Melnik O, Stanković G, Delić N, Vančetović J. Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality. in Chilean Journal of Agricultural Research. 2020;80(4):598-607.
doi:10.4067/S0718-58392020000400598 .

Ignjatović-Micić, Dragana, Kostadinović, Marija, Božinović, Sofija, Đorđević-Melnik, Olivera, Stanković, Goran, Delić, Nenad, Vančetović, Jelena, "Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality" in Chilean Journal of Agricultural Research, 80, no. 4 (2020):598-607,
https://doi.org/10.4067/S0718-58392020000400598 . .

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kravić, Natalija
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/814
AB  - Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing.
AB  - Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Morphological and physiological response of maize seedlings to chilling stress
VL  - 52
IS  - 2
SP  - 689
EP  - 698
DO  - 10.2298/GENSR2002689N
ER  - 

@article{
author = "Nikolić, Ana and Kravić, Natalija and Ristić, Danijela and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing., Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Morphological and physiological response of maize seedlings to chilling stress",
volume = "52",
number = "2",
pages = "689-698",
doi = "10.2298/GENSR2002689N"
}

Nikolić, A., Kravić, N., Ristić, D., Anđelković, V., Marković, K., Vančetović, J.,& Ignjatović-Micić, D.. (2020). Morphological and physiological response of maize seedlings to chilling stress. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 689-698.
https://doi.org/10.2298/GENSR2002689N

Nikolić A, Kravić N, Ristić D, Anđelković V, Marković K, Vančetović J, Ignjatović-Micić D. Morphological and physiological response of maize seedlings to chilling stress. in Genetika. 2020;52(2):689-698.
doi:10.2298/GENSR2002689N .

Nikolić, Ana, Kravić, Natalija, Ristić, Danijela, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, Ignjatović-Micić, Dragana, "Morphological and physiological response of maize seedlings to chilling stress" in Genetika, 52, no. 2 (2020):689-698,
https://doi.org/10.2298/GENSR2002689N . .

TY  - CONF
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Dodig, Dejan
AU  - Kandić, Vesna
AU  - Đorđević-Melnik, Olivera
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1123
AB  - In this research an influence of the strong source restriction meaning cutting off the whole
plants at the first internodes 5 (5DAPt), 10 (10DAPt) and 15 (15DAPt) days after pollination
on grain yield and its corresponding traits in maize were tested. Control represented plants
harvested at full maturity. Four inbred lines were used, two historical ones (Mo17 and B73)
and two commercial ZP inbreds (ZPL and ZPB). The experiment was conducted at Zemun
Polje, Serbia, in 2014 and 2015. The trait of particular importance was the number of kernels
per ear and its average values were 37.73 at 5DAPt, 115.14 at 10DAPt and 175.20 at 15DAPt,
being sufficient for planting next generation of breeding. According to the results obtained,
ZPL represented an improved Lancaster line over Mo17 regarding drought tolerance, that
could not be stated for ZPB over B73. Hybrid among these two lines is drought tolerant due to
heterosis (epistatic effects) or dominant origin of ZPLs good response to drought stress.
Values for seed set and eventually for grain yield per plant were 0.00 for line B73 at 5DAPt in
2015. An improved breeding scheme for increased drought tolerance could be proposed,
namely self-pollination of border plants on high density sown selfing progenies or dihaploid
(DH) lines, cutting off selfed plants at 15 DAP and evaluating their kernel properties. Openpollinated progeny would serve to estimate other important traits for selection. From the
chosen progenies kernels of selfed and cut-off plants should be used for the next generation of
breeding.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings
T1  - The influence of plant cutting on grain yield traits in maize
SP  - 580
EP  - 585
UR  - https://hdl.handle.net/21.15107/rcub_rik_1123
ER  - 

@conference{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Božinović, Sofija and Nikolić, Ana and Dodig, Dejan and Kandić, Vesna and Đorđević-Melnik, Olivera",
year = "2019",
abstract = "In this research an influence of the strong source restriction meaning cutting off the whole
plants at the first internodes 5 (5DAPt), 10 (10DAPt) and 15 (15DAPt) days after pollination
on grain yield and its corresponding traits in maize were tested. Control represented plants
harvested at full maturity. Four inbred lines were used, two historical ones (Mo17 and B73)
and two commercial ZP inbreds (ZPL and ZPB). The experiment was conducted at Zemun
Polje, Serbia, in 2014 and 2015. The trait of particular importance was the number of kernels
per ear and its average values were 37.73 at 5DAPt, 115.14 at 10DAPt and 175.20 at 15DAPt,
being sufficient for planting next generation of breeding. According to the results obtained,
ZPL represented an improved Lancaster line over Mo17 regarding drought tolerance, that
could not be stated for ZPB over B73. Hybrid among these two lines is drought tolerant due to
heterosis (epistatic effects) or dominant origin of ZPLs good response to drought stress.
Values for seed set and eventually for grain yield per plant were 0.00 for line B73 at 5DAPt in
2015. An improved breeding scheme for increased drought tolerance could be proposed,
namely self-pollination of border plants on high density sown selfing progenies or dihaploid
(DH) lines, cutting off selfed plants at 15 DAP and evaluating their kernel properties. Openpollinated progeny would serve to estimate other important traits for selection. From the
chosen progenies kernels of selfed and cut-off plants should be used for the next generation of
breeding.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings",
title = "The influence of plant cutting on grain yield traits in maize",
pages = "580-585",
url = "https://hdl.handle.net/21.15107/rcub_rik_1123"
}

Vančetović, J., Ignjatović-Micić, D., Božinović, S., Nikolić, A., Dodig, D., Kandić, V.,& Đorđević-Melnik, O.. (2019). The influence of plant cutting on grain yield traits in maize. in 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 580-585.
https://hdl.handle.net/21.15107/rcub_rik_1123

Vančetović J, Ignjatović-Micić D, Božinović S, Nikolić A, Dodig D, Kandić V, Đorđević-Melnik O. The influence of plant cutting on grain yield traits in maize. in 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings. 2019;:580-585.
https://hdl.handle.net/21.15107/rcub_rik_1123 .

Vančetović, Jelena, Ignjatović-Micić, Dragana, Božinović, Sofija, Nikolić, Ana, Dodig, Dejan, Kandić, Vesna, Đorđević-Melnik, Olivera, "The influence of plant cutting on grain yield traits in maize" in 10. international Scientific Agriculture Symposium "Agrosym 2019", 03-06.10.2019., Jahorina, Bosnia and Herzegovina - Book of proceedings (2019):580-585,
https://hdl.handle.net/21.15107/rcub_rik_1123 .

TY  - CONF
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
AU  - Kravić, Natalija
AU  - Kovinčić, Anika
AU  - Obradović, Ana
AU  - Stevanović, Milan
AU  - Pavlov, Jovan
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/979
AB  - Despite the huge diversity of maize germplasm, modern maize breeding programme and
agricultural practices decrease the diversity of modern hybrids. Genetic characterization of
maize hybrids allows knowledge of the genetic relationship among them, thus preventing the
risk of increasing uniformity. Because of their high reproducibility, informativeness and easey
application of microsatelites are the most frequently used molecular markers in maize genetic
diversity studies. The aim of our work was to evaluate genetic diversity of maize hybrids by
SSR markers and compare results with their pedigre information. Sixteen polymorphic SSR
(Simple Sequence Repeats) markers were used to characterize 14 maize (Zea mays L.) hybrids
belonging to different breeding programs and FAO groups (from 300 to 800). A total of 53
alleles were found, ranging from two to four alleles. Genetic similarities were calculated in
NTSYSpc2 program package using Jaccard’s coefficient based on binary data (presence or
absence of alleles). The highest value of genetic similarity was 0.80 between H1 and H2,
while the lowest value (0.26) was found between H12 and H13. Cluster analysis was done by
unweighted pair group method (UPGMA) on the basis of genetic similarity matrix.
Dendrogram analysis grouped maize hybrids in one cluster (most of the analyzed genotypes),
one smaller cluster and one branch. The results revealed genetic heterogeneity between analyzed maize hybrids.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina
T1  - Molecular characterisation of maize hybrids
SP  - 211
EP  - 215
UR  - https://hdl.handle.net/21.15107/rcub_rik_979
ER  - 

@conference{
author = "Ristić, Danijela and Kostadinović, Marija and Kravić, Natalija and Kovinčić, Anika and Obradović, Ana and Stevanović, Milan and Pavlov, Jovan",
year = "2019",
abstract = "Despite the huge diversity of maize germplasm, modern maize breeding programme and
agricultural practices decrease the diversity of modern hybrids. Genetic characterization of
maize hybrids allows knowledge of the genetic relationship among them, thus preventing the
risk of increasing uniformity. Because of their high reproducibility, informativeness and easey
application of microsatelites are the most frequently used molecular markers in maize genetic
diversity studies. The aim of our work was to evaluate genetic diversity of maize hybrids by
SSR markers and compare results with their pedigre information. Sixteen polymorphic SSR
(Simple Sequence Repeats) markers were used to characterize 14 maize (Zea mays L.) hybrids
belonging to different breeding programs and FAO groups (from 300 to 800). A total of 53
alleles were found, ranging from two to four alleles. Genetic similarities were calculated in
NTSYSpc2 program package using Jaccard’s coefficient based on binary data (presence or
absence of alleles). The highest value of genetic similarity was 0.80 between H1 and H2,
while the lowest value (0.26) was found between H12 and H13. Cluster analysis was done by
unweighted pair group method (UPGMA) on the basis of genetic similarity matrix.
Dendrogram analysis grouped maize hybrids in one cluster (most of the analyzed genotypes),
one smaller cluster and one branch. The results revealed genetic heterogeneity between analyzed maize hybrids.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina",
title = "Molecular characterisation of maize hybrids",
pages = "211-215",
url = "https://hdl.handle.net/21.15107/rcub_rik_979"
}

Ristić, D., Kostadinović, M., Kravić, N., Kovinčić, A., Obradović, A., Stevanović, M.,& Pavlov, J.. (2019). Molecular characterisation of maize hybrids. in 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 211-215.
https://hdl.handle.net/21.15107/rcub_rik_979

Ristić D, Kostadinović M, Kravić N, Kovinčić A, Obradović A, Stevanović M, Pavlov J. Molecular characterisation of maize hybrids. in 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina. 2019;:211-215.
https://hdl.handle.net/21.15107/rcub_rik_979 .

Ristić, Danijela, Kostadinović, Marija, Kravić, Natalija, Kovinčić, Anika, Obradović, Ana, Stevanović, Milan, Pavlov, Jovan, "Molecular characterisation of maize hybrids" in 10. International scientific agricultural symposium “Agrosym 2019”, 03-06.10.2019. Jahorina, Bosnia and Herzegovina (2019):211-215,
https://hdl.handle.net/21.15107/rcub_rik_979 .

TY  - JOUR
AU  - Dodig, Dejan
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Zorić, Miroslav
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Weigelt-Fischer, Kathleen
AU  - Junker, Astrid
AU  - Altmann, Thomas
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/812
AB  - Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.
PB  - Lausanne : Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress
VL  - 10
SP  - 814
DO  - 10.3389/fpls.2019.00814
ER  - 

@article{
author = "Dodig, Dejan and Božinović, Sofija and Nikolić, Ana and Zorić, Miroslav and Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Weigelt-Fischer, Kathleen and Junker, Astrid and Altmann, Thomas",
year = "2019",
abstract = "Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.",
publisher = "Lausanne : Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress",
volume = "10",
pages = "814",
doi = "10.3389/fpls.2019.00814"
}

Dodig, D., Božinović, S., Nikolić, A., Zorić, M., Vančetović, J., Ignjatović-Micić, D., Delić, N., Weigelt-Fischer, K., Junker, A.,& Altmann, T.. (2019). Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science
Lausanne : Frontiers Media SA., 10, 814.
https://doi.org/10.3389/fpls.2019.00814

Dodig D, Božinović S, Nikolić A, Zorić M, Vančetović J, Ignjatović-Micić D, Delić N, Weigelt-Fischer K, Junker A, Altmann T. Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science. 2019;10:814.
doi:10.3389/fpls.2019.00814 .

Dodig, Dejan, Božinović, Sofija, Nikolić, Ana, Zorić, Miroslav, Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Weigelt-Fischer, Kathleen, Junker, Astrid, Altmann, Thomas, "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress" in Frontiers in Plant Science, 10 (2019):814,
https://doi.org/10.3389/fpls.2019.00814 . .

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/743
AB  - Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije.
AB  - The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Marker assisted backcross breeding in Maize Research Institute Zemun Polje
T1  - Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje
VL  - 25
IS  - 1
SP  - 41
EP  - 47
DO  - 10.5937/SelSem1901041K
ER  - 

@article{
author = "Kostadinović, Marija and Nikolić, Ana and Ristić, Danijela and Božinović, Sofija and Đorđević-Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije., The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Marker assisted backcross breeding in Maize Research Institute Zemun Polje, Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje",
volume = "25",
number = "1",
pages = "41-47",
doi = "10.5937/SelSem1901041K"
}

Kostadinović, M., Nikolić, A., Ristić, D., Božinović, S., Đorđević-Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2019). Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 41-47.
https://doi.org/10.5937/SelSem1901041K

Kostadinović M, Nikolić A, Ristić D, Božinović S, Đorđević-Melnik O, Ignjatović-Micić D, Vančetović J. Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo. 2019;25(1):41-47.
doi:10.5937/SelSem1901041K .

Kostadinović, Marija, Nikolić, Ana, Ristić, Danijela, Božinović, Sofija, Đorđević-Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted backcross breeding in Maize Research Institute Zemun Polje" in Selekcija i semenarstvo, 25, no. 1 (2019):41-47,
https://doi.org/10.5937/SelSem1901041K . .

TY  - JOUR
AU  - Babić, Vojka
AU  - Kravić, Natalija
AU  - Srdić, Jelena
AU  - Popović, Aleksandar
AU  - Nikolić, Ana
AU  - Zivić, Jovana
AU  - Miritescu, Mihai
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/731
AB  - White maize had an important role in human nutrition throughout the Western Balkan region. A great number of farmers (approximately 86% in Serbia) still grow traditional white Open Pollinated Varieties (OPVs) for human diet. Out of 2217 maize landraces stored within Maize Research Institute "Zemun Polje" (MRIZP) gene bank, more than 700 are white kernel accessions. Eighteen white maize landraces, collected in the Western Balkan region in different periods, were selected for the present study. By the evaluation of agromorphological performances and the basic chemical composition of grain, the objective was to determine the importance of landraces conserved under in situ and ex situ conditions, to be used as the initial material for breeding. The idea was to determine whether the varieties conserved under ex situ conditions (collected from the same or different regions) distinguish from varieties permanently grown in the region of western Serbia. It was found that varieties permanently grown in the western Serbia were more similar to varieties of the ex situ collection from eastern Bosnia and Herzegovina than to varieties collected in western Serbia 50 years ago. The comparison between OPVs and modern white maize hybrids for the basic chemical composition of grain showed the significant potential of OPVs for nutritive grain quality improvement. Increased organic production creates new possibilities for more intensive incorporation of OPVs into this agricultural system. New opportunities for the cooperation and share of responsibilities among breeders, farmers and genetic resources managers are initiated, to be used for in situ maize genetic resources conservation improvement. More detailed characterisation and evaluation of the white maize landraces will offer an explanation of what has motivated our farmers to maintain the OPVs production through centuries, despite the availability of modern hybrids.
PB  - Natl Agricultural Research & Development Inst, Fundulea
T2  - Romanian Agricultural Research
T1  - Grain quality within ex situ and in situ conserved traditional white maize landraces
VL  - 35
SP  - 129
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_rik_731
ER  - 

@article{
author = "Babić, Vojka and Kravić, Natalija and Srdić, Jelena and Popović, Aleksandar and Nikolić, Ana and Zivić, Jovana and Miritescu, Mihai",
year = "2018",
abstract = "White maize had an important role in human nutrition throughout the Western Balkan region. A great number of farmers (approximately 86% in Serbia) still grow traditional white Open Pollinated Varieties (OPVs) for human diet. Out of 2217 maize landraces stored within Maize Research Institute "Zemun Polje" (MRIZP) gene bank, more than 700 are white kernel accessions. Eighteen white maize landraces, collected in the Western Balkan region in different periods, were selected for the present study. By the evaluation of agromorphological performances and the basic chemical composition of grain, the objective was to determine the importance of landraces conserved under in situ and ex situ conditions, to be used as the initial material for breeding. The idea was to determine whether the varieties conserved under ex situ conditions (collected from the same or different regions) distinguish from varieties permanently grown in the region of western Serbia. It was found that varieties permanently grown in the western Serbia were more similar to varieties of the ex situ collection from eastern Bosnia and Herzegovina than to varieties collected in western Serbia 50 years ago. The comparison between OPVs and modern white maize hybrids for the basic chemical composition of grain showed the significant potential of OPVs for nutritive grain quality improvement. Increased organic production creates new possibilities for more intensive incorporation of OPVs into this agricultural system. New opportunities for the cooperation and share of responsibilities among breeders, farmers and genetic resources managers are initiated, to be used for in situ maize genetic resources conservation improvement. More detailed characterisation and evaluation of the white maize landraces will offer an explanation of what has motivated our farmers to maintain the OPVs production through centuries, despite the availability of modern hybrids.",
publisher = "Natl Agricultural Research & Development Inst, Fundulea",
journal = "Romanian Agricultural Research",
title = "Grain quality within ex situ and in situ conserved traditional white maize landraces",
volume = "35",
pages = "129-140",
url = "https://hdl.handle.net/21.15107/rcub_rik_731"
}

Babić, V., Kravić, N., Srdić, J., Popović, A., Nikolić, A., Zivić, J.,& Miritescu, M.. (2018). Grain quality within ex situ and in situ conserved traditional white maize landraces. in Romanian Agricultural Research
Natl Agricultural Research & Development Inst, Fundulea., 35, 129-140.
https://hdl.handle.net/21.15107/rcub_rik_731

Babić V, Kravić N, Srdić J, Popović A, Nikolić A, Zivić J, Miritescu M. Grain quality within ex situ and in situ conserved traditional white maize landraces. in Romanian Agricultural Research. 2018;35:129-140.
https://hdl.handle.net/21.15107/rcub_rik_731 .

Babić, Vojka, Kravić, Natalija, Srdić, Jelena, Popović, Aleksandar, Nikolić, Ana, Zivić, Jovana, Miritescu, Mihai, "Grain quality within ex situ and in situ conserved traditional white maize landraces" in Romanian Agricultural Research, 35 (2018):129-140,
https://hdl.handle.net/21.15107/rcub_rik_731 .

TY  - JOUR
AU  - Kravić, Natalija
AU  - Babić, Vojka
AU  - Srdić, Jelena
AU  - Mesarović, Jelena
AU  - Anđelković, Violeta
AU  - Hadži-Tašković-Šukalović, Vesna
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/712
AB  - Twenty-six maize landraces were tested in order to evaluate maize seedling performance as an index for drought tolerance in adult plants. Samples were subjected to polyethylene glycol-induced osmotic stress at the early seedling stage. Grain yield was obtained in field experiments under well-watered (OC) and a combination of drought and high plant density (HD) conditions. Osmotic stress caused a reduction in seedling growth (length, fresh and dry weight), and increase in the shoot and in particular the root proline contents in the majority of landraces, and variations in root peroxidase (POD) activity. Genotypes displaying more pronounced root growth reduction and higher proline contents exhibited decreased POD activity under osmotic stress. Direct positive correlations between the proline content and growth inhibition, and between the proline and soluble protein content were established. Correlations between the changes in POD activity and growth parameters were significant and positive, and significant but negative with the changes in the proline content. In the field, water stress led to a reduction in grain yield in all of the tested landraces. Correlations between grain yield from both experimental sets (OC and HD) and osmotic-induced changes in seedling root growth were negative, which was opposite to the highly significant and positive correlations between the changes in the seedling root proline content and yield. Also, genotypes with the highest seedling root proline content increase under osmotic stress, exhibited the highest stress tolerance index (STI) based on grain yield achieved under both field conditions. Our results indicate that lower changes in POD activity and especially an increased proline content after exposure to osmotic stress during the early seedling stage could be considered as useful indices to facilitate selection efficiency for drought tolerance in adult plants.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Maize seedling performance as a potential index for drought tolerance
VL  - 70
IS  - 1
SP  - 167
EP  - 177
DO  - 10.2298/ABS170504036K
ER  - 

@article{
author = "Kravić, Natalija and Babić, Vojka and Srdić, Jelena and Mesarović, Jelena and Anđelković, Violeta and Hadži-Tašković-Šukalović, Vesna",
year = "2018",
abstract = "Twenty-six maize landraces were tested in order to evaluate maize seedling performance as an index for drought tolerance in adult plants. Samples were subjected to polyethylene glycol-induced osmotic stress at the early seedling stage. Grain yield was obtained in field experiments under well-watered (OC) and a combination of drought and high plant density (HD) conditions. Osmotic stress caused a reduction in seedling growth (length, fresh and dry weight), and increase in the shoot and in particular the root proline contents in the majority of landraces, and variations in root peroxidase (POD) activity. Genotypes displaying more pronounced root growth reduction and higher proline contents exhibited decreased POD activity under osmotic stress. Direct positive correlations between the proline content and growth inhibition, and between the proline and soluble protein content were established. Correlations between the changes in POD activity and growth parameters were significant and positive, and significant but negative with the changes in the proline content. In the field, water stress led to a reduction in grain yield in all of the tested landraces. Correlations between grain yield from both experimental sets (OC and HD) and osmotic-induced changes in seedling root growth were negative, which was opposite to the highly significant and positive correlations between the changes in the seedling root proline content and yield. Also, genotypes with the highest seedling root proline content increase under osmotic stress, exhibited the highest stress tolerance index (STI) based on grain yield achieved under both field conditions. Our results indicate that lower changes in POD activity and especially an increased proline content after exposure to osmotic stress during the early seedling stage could be considered as useful indices to facilitate selection efficiency for drought tolerance in adult plants.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Maize seedling performance as a potential index for drought tolerance",
volume = "70",
number = "1",
pages = "167-177",
doi = "10.2298/ABS170504036K"
}

Kravić, N., Babić, V., Srdić, J., Mesarović, J., Anđelković, V.,& Hadži-Tašković-Šukalović, V.. (2018). Maize seedling performance as a potential index for drought tolerance. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 70(1), 167-177.
https://doi.org/10.2298/ABS170504036K

Kravić N, Babić V, Srdić J, Mesarović J, Anđelković V, Hadži-Tašković-Šukalović V. Maize seedling performance as a potential index for drought tolerance. in Archives of Biological Sciences. 2018;70(1):167-177.
doi:10.2298/ABS170504036K .

Kravić, Natalija, Babić, Vojka, Srdić, Jelena, Mesarović, Jelena, Anđelković, Violeta, Hadži-Tašković-Šukalović, Vesna, "Maize seedling performance as a potential index for drought tolerance" in Archives of Biological Sciences, 70, no. 1 (2018):167-177,
https://doi.org/10.2298/ABS170504036K . .

TY  - CONF
AU  - Ristić, Danijela
AU  - Ignjatovic-Micic, Dragana
AU  - Mladenović Drinić, Snežana
AU  - Obradović, Ana
AU  - Kostadinović, Marija
AU  - Stevanović, Milan
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/976
AB  - One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
T1  - Estimation of genetic diversity among maize inbred lines
SP  - 280
EP  - 284
UR  - https://hdl.handle.net/21.15107/rcub_rik_976
ER  - 

@conference{
author = "Ristić, Danijela and Ignjatovic-Micic, Dragana and Mladenović Drinić, Snežana and Obradović, Ana and Kostadinović, Marija and Stevanović, Milan and Stanković, Goran",
year = "2018",
abstract = "One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina",
title = "Estimation of genetic diversity among maize inbred lines",
pages = "280-284",
url = "https://hdl.handle.net/21.15107/rcub_rik_976"
}

Ristić, D., Ignjatovic-Micic, D., Mladenović Drinić, S., Obradović, A., Kostadinović, M., Stevanović, M.,& Stanković, G.. (2018). Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 280-284.
https://hdl.handle.net/21.15107/rcub_rik_976

Ristić D, Ignjatovic-Micic D, Mladenović Drinić S, Obradović A, Kostadinović M, Stevanović M, Stanković G. Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina. 2018;:280-284.
https://hdl.handle.net/21.15107/rcub_rik_976 .

Ristić, Danijela, Ignjatovic-Micic, Dragana, Mladenović Drinić, Snežana, Obradović, Ana, Kostadinović, Marija, Stevanović, Milan, Stanković, Goran, "Estimation of genetic diversity among maize inbred lines" in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina (2018):280-284,
https://hdl.handle.net/21.15107/rcub_rik_976 .

TY  - JOUR
AU  - Čamdžija, Zoran
AU  - Dragičević, Vesna
AU  - Vančetović, Jelena
AU  - Stevanović, Milan
AU  - Pavlov, Jovan
AU  - Filipović, Milomir
AU  - Ignjatović-Micić, Dragana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/727
AB  - A set of fifteen elite inbred lines of maize (used as mothers) and three tester inbred lines (used as fathers) were investigated using line x tester statistical model, including both hybrids and inbreds. Four traits were measured: grain yield, 1000 kernel weight, phytic phosphorus (Pphy) and inorganic phosphorus (P-i) in the kernel. P-phy content among hybrids ranged from 2.342 to 4.812 g kg(-1) and P-i content from 0.562 to 2.340 g kg(-1), while among inbreds (lines and testers) they ranged from 2.503 to 4.180 g kg(-1) and from 0.587 to 1.629 g kg(-1), respectively. Correlations between the four traits allow breeding for high P, and low Pphy, as well as for both high P-i and phytate, without compromising grain yield. Correlation for hybrids between P-phy and P-i was 0.185 (p lt 0.05) and for inbreds 0.142 (non-significant). General combining ability / special combining ability (GCA/SCA) values for all the traits were below 1 (very low) indicated non-additive inheritance. In the investigated set of genotypes, multiple selection indices should be used for simultaneous improvement of grain yield and phosphorus profile of maize grain.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel
VL  - 50
IS  - 1
SP  - 299
EP  - 314
DO  - 10.2298/GENSR1801299C
ER  - 

@article{
author = "Čamdžija, Zoran and Dragičević, Vesna and Vančetović, Jelena and Stevanović, Milan and Pavlov, Jovan and Filipović, Milomir and Ignjatović-Micić, Dragana",
year = "2018",
abstract = "A set of fifteen elite inbred lines of maize (used as mothers) and three tester inbred lines (used as fathers) were investigated using line x tester statistical model, including both hybrids and inbreds. Four traits were measured: grain yield, 1000 kernel weight, phytic phosphorus (Pphy) and inorganic phosphorus (P-i) in the kernel. P-phy content among hybrids ranged from 2.342 to 4.812 g kg(-1) and P-i content from 0.562 to 2.340 g kg(-1), while among inbreds (lines and testers) they ranged from 2.503 to 4.180 g kg(-1) and from 0.587 to 1.629 g kg(-1), respectively. Correlations between the four traits allow breeding for high P, and low Pphy, as well as for both high P-i and phytate, without compromising grain yield. Correlation for hybrids between P-phy and P-i was 0.185 (p lt 0.05) and for inbreds 0.142 (non-significant). General combining ability / special combining ability (GCA/SCA) values for all the traits were below 1 (very low) indicated non-additive inheritance. In the investigated set of genotypes, multiple selection indices should be used for simultaneous improvement of grain yield and phosphorus profile of maize grain.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel",
volume = "50",
number = "1",
pages = "299-314",
doi = "10.2298/GENSR1801299C"
}

Čamdžija, Z., Dragičević, V., Vančetović, J., Stevanović, M., Pavlov, J., Filipović, M.,& Ignjatović-Micić, D.. (2018). Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel. in Genetika
Društvo genetičara Srbije, Beograd., 50(1), 299-314.
https://doi.org/10.2298/GENSR1801299C

Čamdžija Z, Dragičević V, Vančetović J, Stevanović M, Pavlov J, Filipović M, Ignjatović-Micić D. Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel. in Genetika. 2018;50(1):299-314.
doi:10.2298/GENSR1801299C .

Čamdžija, Zoran, Dragičević, Vesna, Vančetović, Jelena, Stevanović, Milan, Pavlov, Jovan, Filipović, Milomir, Ignjatović-Micić, Dragana, "Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel" in Genetika, 50, no. 1 (2018):299-314,
https://doi.org/10.2298/GENSR1801299C . .

TY  - CONF
AU  - Kravic, Natalija
AU  - Mesarović, Jelena
AU  - Dragičević, Vesna
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Pećinar, Ilinka
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/987
AB  - Maize grain carotenoids are among the most abundant in cereals, exhibiting considerable diversity in the composition profiles. In this experiment, HPLC-DAD was used for lutein and zeaxanthin (predominant carotenoids) and β-carotene (proVA carotenoid) determination in flour of
whole maize kernel. In addition, Raman spectroscopy, as a non-destructive method for pretreatment-free and rapid in situ screening of carotenoids status in different kernel regions, was applied.
Six yellow maize inbred lines (L1-L6) were evaluated. After the spectral normalization, deconvolution was performed on three individual bands in three observed regions on kernel longitudinal
section (aleurone cell layer, floury and vitreous endosperm), for all genotypes. It was found that
the mean fitted area and intensity of three main carotenoid bands were the highest at 1520 cm-1,
medium at 1155 cm-1 and the lowest for the band at 1007 cm-1, as spectral features used to identify carotenoids and quantify relative carotenoid concentration. Both the highest values for mean
band fitted area and intensity observed in the floury endosperm, and the lowest in the aleurone
cell layer, were recorded in the same regions for all genotypes evaluated. L1 and L2 exhibited the
highest values, while L3 and L4 exhibited the lowest values for the average and total band area.
Significant and negative correlation between carotenoid content quantified by HPLC-DAD and
bands area fit for vitreous endosperm region obtained by Raman spectroscopy (-0.847; p≤0.05),
and between carotenoid content and total bands area (-0.898; p≤0.05) indicated the competition
for carbon supplies reflected through increased starch reposition over carotenoids accumulation.
PB  - Belgrade : Serbian plant physiology society
C3  - 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts
T1  - Application of different methods for measuring carotenoid status in maize grain
UR  - https://hdl.handle.net/21.15107/rcub_rik_987
ER  - 

@conference{
author = "Kravic, Natalija and Mesarović, Jelena and Dragičević, Vesna and Ristić, Danijela and Anđelković, Violeta and Pećinar, Ilinka",
year = "2018",
abstract = "Maize grain carotenoids are among the most abundant in cereals, exhibiting considerable diversity in the composition profiles. In this experiment, HPLC-DAD was used for lutein and zeaxanthin (predominant carotenoids) and β-carotene (proVA carotenoid) determination in flour of
whole maize kernel. In addition, Raman spectroscopy, as a non-destructive method for pretreatment-free and rapid in situ screening of carotenoids status in different kernel regions, was applied.
Six yellow maize inbred lines (L1-L6) were evaluated. After the spectral normalization, deconvolution was performed on three individual bands in three observed regions on kernel longitudinal
section (aleurone cell layer, floury and vitreous endosperm), for all genotypes. It was found that
the mean fitted area and intensity of three main carotenoid bands were the highest at 1520 cm-1,
medium at 1155 cm-1 and the lowest for the band at 1007 cm-1, as spectral features used to identify carotenoids and quantify relative carotenoid concentration. Both the highest values for mean
band fitted area and intensity observed in the floury endosperm, and the lowest in the aleurone
cell layer, were recorded in the same regions for all genotypes evaluated. L1 and L2 exhibited the
highest values, while L3 and L4 exhibited the lowest values for the average and total band area.
Significant and negative correlation between carotenoid content quantified by HPLC-DAD and
bands area fit for vitreous endosperm region obtained by Raman spectroscopy (-0.847; p≤0.05),
and between carotenoid content and total bands area (-0.898; p≤0.05) indicated the competition
for carbon supplies reflected through increased starch reposition over carotenoids accumulation.",
publisher = "Belgrade : Serbian plant physiology society",
journal = "3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts",
title = "Application of different methods for measuring carotenoid status in maize grain",
url = "https://hdl.handle.net/21.15107/rcub_rik_987"
}

Kravic, N., Mesarović, J., Dragičević, V., Ristić, D., Anđelković, V.,& Pećinar, I.. (2018). Application of different methods for measuring carotenoid status in maize grain. in 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts
Belgrade : Serbian plant physiology society..
https://hdl.handle.net/21.15107/rcub_rik_987

Kravic N, Mesarović J, Dragičević V, Ristić D, Anđelković V, Pećinar I. Application of different methods for measuring carotenoid status in maize grain. in 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts. 2018;.
https://hdl.handle.net/21.15107/rcub_rik_987 .

Kravic, Natalija, Mesarović, Jelena, Dragičević, Vesna, Ristić, Danijela, Anđelković, Violeta, Pećinar, Ilinka, "Application of different methods for measuring carotenoid status in maize grain" in 3. International conference on plant biology (22. SPPS meeting), 09-12.06.2018., Belgrade - Book of abstracts (2018),
https://hdl.handle.net/21.15107/rcub_rik_987 .

TY  - JOUR
AU  - Milašinović-Šeremešić, Marija
AU  - Radosavljević, Milica
AU  - Terzić, Dušanka
AU  - Nikolić, Valentina
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/693
AB  - According to the estimation of leading experts, the maize utilisation for the production of silage from the whole plant, wet ear and wet grain should be one of the most important forms of its application in animal nutrition. Maize a major forage crop, because the highest accumulation of solar energy per area unit is accomplished by maize production and ensiling. At the Maize Research Institute, Zemun Polje, selection, testing and evaluation of silage maize. The aim of this study was to draw attention to this extremely significant field of science and studies on the utilisation of the whole maize plant (biomass) in feeding of domestic animals, primarily ruminants. This paper presents and discusses previous results and achievements of long-term scientific researches on the improvement of maize plant utilisation in making good-quality silage not only in our country but also in the world.
AB  - Prema procenama vodećih stručnjaka korišćenje kukuruza za proizvodnju silaže od cele biljke, vlažnog klipa i vlažnog zrna trebalo bi da bude jedan od značajnijih oblika njegove primene u ishrani životinja. Kukuruz je najvažnija krmna biljka jer se njenom proizvodnjom i siliranjem postiže najviša akumulacija sunčeve energije po jedinici površine. U Institutu za kukuruz 'Zemun Polje' paralelno sa ispitivanjem i ocenom kvaliteta hibrida kukuruza namenjenih proizvodnji zrna vrši se selekcija, ispitivanje i ocena i silažnih formi kukuruza, odnosno upotrebne vrednosti i kvaliteta kukuruzne biljke za ishranu životinja. Kao rezultat ovog naučnoistraživačkog rada poslednjih nekoliko decenija selekcionisani su hibridi kukuruza koji su na osnovu ispitivanih parametara kvaliteta za silažne forme kukuruza visokog kvalitete. Karakteriše ih: visok prinos ukupne suve i ukupne svarljive suve materije po hektaru, visoko učešće suve materije klipa u ukupnom prinosu suve materije i visoka svarljivost suve materije cele biljke kukuruza. Cilj ovog rada bio je da se skrene pažnja na ovu izuzetno značajnu oblast nauke i istraživanja upotrebe cele kukuruzne biljke (biomase) u ishrani domaćih životinja, prvenstveno preživara. Prikazani su i diskutovani dosadašnji rezultati i dostignuća višegodišnjeg naučnoistraživačkog rada na unapređenju korišćenja kukuruzne biljke za spremanje kvalitetne silaže u našoj zemlji kao i u svetu. Opisan je kukuruz kao sirovina za proizvodnju i spremanje silaže od cele kukuruzne biljke kao kvalitetne kabaste hrane za ishranu domaćih životinja.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - The utilisable value of the maize plant (biomass) for silage
T1  - Upotrebna vrednost kukuruzne biljke (biomase) za silažu
VL  - 21
IS  - 2
SP  - 86
EP  - 90
DO  - 10.5937/JPEA1702086S
ER  - 

@article{
author = "Milašinović-Šeremešić, Marija and Radosavljević, Milica and Terzić, Dušanka and Nikolić, Valentina",
year = "2017",
abstract = "According to the estimation of leading experts, the maize utilisation for the production of silage from the whole plant, wet ear and wet grain should be one of the most important forms of its application in animal nutrition. Maize a major forage crop, because the highest accumulation of solar energy per area unit is accomplished by maize production and ensiling. At the Maize Research Institute, Zemun Polje, selection, testing and evaluation of silage maize. The aim of this study was to draw attention to this extremely significant field of science and studies on the utilisation of the whole maize plant (biomass) in feeding of domestic animals, primarily ruminants. This paper presents and discusses previous results and achievements of long-term scientific researches on the improvement of maize plant utilisation in making good-quality silage not only in our country but also in the world., Prema procenama vodećih stručnjaka korišćenje kukuruza za proizvodnju silaže od cele biljke, vlažnog klipa i vlažnog zrna trebalo bi da bude jedan od značajnijih oblika njegove primene u ishrani životinja. Kukuruz je najvažnija krmna biljka jer se njenom proizvodnjom i siliranjem postiže najviša akumulacija sunčeve energije po jedinici površine. U Institutu za kukuruz 'Zemun Polje' paralelno sa ispitivanjem i ocenom kvaliteta hibrida kukuruza namenjenih proizvodnji zrna vrši se selekcija, ispitivanje i ocena i silažnih formi kukuruza, odnosno upotrebne vrednosti i kvaliteta kukuruzne biljke za ishranu životinja. Kao rezultat ovog naučnoistraživačkog rada poslednjih nekoliko decenija selekcionisani su hibridi kukuruza koji su na osnovu ispitivanih parametara kvaliteta za silažne forme kukuruza visokog kvalitete. Karakteriše ih: visok prinos ukupne suve i ukupne svarljive suve materije po hektaru, visoko učešće suve materije klipa u ukupnom prinosu suve materije i visoka svarljivost suve materije cele biljke kukuruza. Cilj ovog rada bio je da se skrene pažnja na ovu izuzetno značajnu oblast nauke i istraživanja upotrebe cele kukuruzne biljke (biomase) u ishrani domaćih životinja, prvenstveno preživara. Prikazani su i diskutovani dosadašnji rezultati i dostignuća višegodišnjeg naučnoistraživačkog rada na unapređenju korišćenja kukuruzne biljke za spremanje kvalitetne silaže u našoj zemlji kao i u svetu. Opisan je kukuruz kao sirovina za proizvodnju i spremanje silaže od cele kukuruzne biljke kao kvalitetne kabaste hrane za ishranu domaćih životinja.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "The utilisable value of the maize plant (biomass) for silage, Upotrebna vrednost kukuruzne biljke (biomase) za silažu",
volume = "21",
number = "2",
pages = "86-90",
doi = "10.5937/JPEA1702086S"
}

Milašinović-Šeremešić, M., Radosavljević, M., Terzić, D.,& Nikolić, V.. (2017). The utilisable value of the maize plant (biomass) for silage. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 21(2), 86-90.
https://doi.org/10.5937/JPEA1702086S

Milašinović-Šeremešić M, Radosavljević M, Terzić D, Nikolić V. The utilisable value of the maize plant (biomass) for silage. in Journal on Processing and Energy in Agriculture. 2017;21(2):86-90.
doi:10.5937/JPEA1702086S .

Milašinović-Šeremešić, Marija, Radosavljević, Milica, Terzić, Dušanka, Nikolić, Valentina, "The utilisable value of the maize plant (biomass) for silage" in Journal on Processing and Energy in Agriculture, 21, no. 2 (2017):86-90,
https://doi.org/10.5937/JPEA1702086S . .

TY  - JOUR
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Nikolić, Ana
AU  - Milivojević, Marija
AU  - Srdić, Jelena
AU  - Popović, Aleksandar
AU  - Kravić, Natalija
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/658
AB  - Maintenance of the original accessions identity and integrity is one of the priorities among genebank activities. Different factors related to conservation may result in accessions disruption. Regeneration is the most frequent critical point in this process, due to bottlenecks, inbreeding, random genetic drift and unintentional mixing or contamination. On the other hand, genetic drift may occur due to seed viability loss. Therefore, it is very important to establish the balance between the frequency of regeneration and the duration of accession conservation. The aim of the present study was to estimate whether the identity of accessions regenerated after 27 years of medium-term conservation was disrupted. Phenotypic markers were applied on three Maize Research Institute "Zemun Polje" (MRIZP) genebank maize landraces (K2026, K768 and K86), differing in seed viability, kernel type and effective population size. It was estimated that, after the regeneration, there had been no significant changes in the landrace K2026. There were some parameters indicating that genetic drift had occurred in the landrace K768, and that there had been even a certain degree of inbreeding in the landrace K86. According to the results, accession K2026 could still be kept under the same ID number. Due to the genuine identity disruption, assignment of new ID numbers for K768 and K86 should be suggested.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Disruption of genetic identity for genebank maize accessions during conservation
VL  - 49
IS  - 3
SP  - 853
EP  - 864
DO  - 10.2298/GENSR1703853B
ER  - 

@article{
author = "Babić, Vojka and Anđelković, Violeta and Nikolić, Ana and Milivojević, Marija and Srdić, Jelena and Popović, Aleksandar and Kravić, Natalija",
year = "2017",
abstract = "Maintenance of the original accessions identity and integrity is one of the priorities among genebank activities. Different factors related to conservation may result in accessions disruption. Regeneration is the most frequent critical point in this process, due to bottlenecks, inbreeding, random genetic drift and unintentional mixing or contamination. On the other hand, genetic drift may occur due to seed viability loss. Therefore, it is very important to establish the balance between the frequency of regeneration and the duration of accession conservation. The aim of the present study was to estimate whether the identity of accessions regenerated after 27 years of medium-term conservation was disrupted. Phenotypic markers were applied on three Maize Research Institute "Zemun Polje" (MRIZP) genebank maize landraces (K2026, K768 and K86), differing in seed viability, kernel type and effective population size. It was estimated that, after the regeneration, there had been no significant changes in the landrace K2026. There were some parameters indicating that genetic drift had occurred in the landrace K768, and that there had been even a certain degree of inbreeding in the landrace K86. According to the results, accession K2026 could still be kept under the same ID number. Due to the genuine identity disruption, assignment of new ID numbers for K768 and K86 should be suggested.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Disruption of genetic identity for genebank maize accessions during conservation",
volume = "49",
number = "3",
pages = "853-864",
doi = "10.2298/GENSR1703853B"
}

Babić, V., Anđelković, V., Nikolić, A., Milivojević, M., Srdić, J., Popović, A.,& Kravić, N.. (2017). Disruption of genetic identity for genebank maize accessions during conservation. in Genetika
Društvo genetičara Srbije, Beograd., 49(3), 853-864.
https://doi.org/10.2298/GENSR1703853B

Babić V, Anđelković V, Nikolić A, Milivojević M, Srdić J, Popović A, Kravić N. Disruption of genetic identity for genebank maize accessions during conservation. in Genetika. 2017;49(3):853-864.
doi:10.2298/GENSR1703853B .

Babić, Vojka, Anđelković, Violeta, Nikolić, Ana, Milivojević, Marija, Srdić, Jelena, Popović, Aleksandar, Kravić, Natalija, "Disruption of genetic identity for genebank maize accessions during conservation" in Genetika, 49, no. 3 (2017):853-864,
https://doi.org/10.2298/GENSR1703853B . .

TY  - JOUR
AU  - Mesarović, Jelena
AU  - Dragičević, Vesna
AU  - Mladenović Drinić, Snežana
AU  - Ristić, Danijela
AU  - Kravić, Natalija
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/678
AB  - Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Determination of free phenolic acids from leaves within different colored maize
VL  - 82
IS  - 1
SP  - 63
EP  - 72
DO  - 10.2298/JSC160512104M
ER  - 

@article{
author = "Mesarović, Jelena and Dragičević, Vesna and Mladenović Drinić, Snežana and Ristić, Danijela and Kravić, Natalija",
year = "2017",
abstract = "Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have only been a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this investigation was to evaluate the content of free phenolic acids in the leaves of fifteen different maize inbred lines. Reverse-phase, high performance liquid chromatography (RP-HPLC), with a photodiode array detector (DAD), was performed. Under the optimized chromatographic conditions, referring to short time of sample preparation, small quantities of solvent and direct injection of the extract into HPLC, phenolic acids (i.e., gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method could be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate parameters: linearity, limit of detection and quantification, accuracy and precision. The obtained results showed that the most abundant free phenolic acid was p-coumaric acid (23.57 mu g g(-1) dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 mu g g(-1) dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of a link.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Determination of free phenolic acids from leaves within different colored maize",
volume = "82",
number = "1",
pages = "63-72",
doi = "10.2298/JSC160512104M"
}

Mesarović, J., Dragičević, V., Mladenović Drinić, S., Ristić, D.,& Kravić, N.. (2017). Determination of free phenolic acids from leaves within different colored maize. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 82(1), 63-72.
https://doi.org/10.2298/JSC160512104M

Mesarović J, Dragičević V, Mladenović Drinić S, Ristić D, Kravić N. Determination of free phenolic acids from leaves within different colored maize. in Journal of the Serbian Chemical Society. 2017;82(1):63-72.
doi:10.2298/JSC160512104M .

Mesarović, Jelena, Dragičević, Vesna, Mladenović Drinić, Snežana, Ristić, Danijela, Kravić, Natalija, "Determination of free phenolic acids from leaves within different colored maize" in Journal of the Serbian Chemical Society, 82, no. 1 (2017):63-72,
https://doi.org/10.2298/JSC160512104M . .

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Trbović, Dejana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/684
AB  - A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.
PB  - Univ Sao Paolo, Cerquera Cesar
T2  - Scientia Agricola
T1  - Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends
VL  - 74
IS  - 6
SP  - 461
EP  - 468
DO  - 10.1590/1678-992X-2016-0302
ER  - 

@article{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Božinović, Sofija and Nikolić, Ana and Kostadinović, Marija and Trbović, Dejana",
year = "2017",
abstract = "A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.",
publisher = "Univ Sao Paolo, Cerquera Cesar",
journal = "Scientia Agricola",
title = "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends",
volume = "74",
number = "6",
pages = "461-468",
doi = "10.1590/1678-992X-2016-0302"
}

Vančetović, J., Ignjatović-Micić, D., Delić, N., Božinović, S., Nikolić, A., Kostadinović, M.,& Trbović, D.. (2017). Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola
Univ Sao Paolo, Cerquera Cesar., 74(6), 461-468.
https://doi.org/10.1590/1678-992X-2016-0302

Vančetović J, Ignjatović-Micić D, Delić N, Božinović S, Nikolić A, Kostadinović M, Trbović D. Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola. 2017;74(6):461-468.
doi:10.1590/1678-992X-2016-0302 .

Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Božinović, Sofija, Nikolić, Ana, Kostadinović, Marija, Trbović, Dejana, "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends" in Scientia Agricola, 74, no. 6 (2017):461-468,
https://doi.org/10.1590/1678-992X-2016-0302 . .