TY  - JOUR
AU  - Djukić-Vuković, Aleksandra
AU  - Mladenović, Dragana
AU  - Nikolić, Valentina
AU  - Kocic-Tanackov, Suncica
AU  - Pejin, Jelena
AU  - Mojović, Ljiljana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/860
AB  - Stillage is a main by-product of the bioethanol industry and, depending on the
origin of substrates for bioethanol production, it can be a significant pollutant
affecting the profitability of bioethanol production. Directing the stillage towards
the production of bio-based chemicals or high-quality feed is a preferred stra-
tegy. In this paper, a complete utilization of stillages of different origins was
assessed. Thin stillages from bioethanol production from molasses, wasted
bread and corn were chemically characterized, evaluated and compared as
substrates for lactic acid (LA) and probiotic biomass production by Lactobacil-
lus rhamnosus ATCC 7469, while solid fractions of wasted bread and corn stil-
lages were analyzed for feed. The impact of pH control using CaCO 3 or NaOH
was also examined, both in terms of LA production and valorization of the
remains generated in each process.A maximal LA productivity of 1.14 g/(L h)
was obtained on thin wasted bread stillage with pH control by NaOH while the
number of viable probiotic bacterial cells was above 109 CFU/mL. The com-
position of the solid fraction of the wasted bread stillage was complementary
with the needs of monogastric animals, while the solid fraction of corn stillage
was more adequate for the nutritional requirements of ruminants.
AB  - Džibra je najznačajniji otpadni ili sporedni proizvod industrije bioetanola. U zavisnosti od
porekla supstrata koji se koriste u proizvodnji bioetanola, može biti značajan zagađivač,
pa utiče na profitabilnost proizvodnje bioetanola. Upotreba džibre u biotehnološkim
postupcima proizvodnje hemikalija ili visoko vredne stočne hrane je poželjna strategija
za smanjenje negativnih efekata džibre na životnu sredinu i profitabilnost proizvodn je
bioetanola. U ovom radu je analizirana mogućnost kompletnog iskorišćenja džibre.
Tečne džibre iz proizvodnje bioetanola na melasi, otpadnom hlebu i kukuruzu su
hemijski okarakterisane i upoređene kao supstrati za proizvodnju mlečne kiseline (MK) i
probiotske biomase soja Lactobacillus rhamnosus ATCC 7469, dok je čvrsti ostatak
hlebne i kukuruzne džibre analiziran kao hrana za životinje. Takođe je ispitan uticaj pH
kontrole pomoću CaCO3 ili NaOH sa aspekta proizvodnje MK i sa aspekta iskorišćenja
ostataka iz oba procesa. Maksimalna produktivnost MK od 1,14 g/(L h) je dobijena na
tečnoj džibri iz otpadnog hleba sa pH kontorolom pomoću NaOH, dok je broj živih pro-
biotskih bakterija bio preko 10 9 CFU/mL. Rezultati su pokazali da je sastav čvrste frak-
cije otpadne hlebne džibre komplementaran sa potrebama monogastričnih životinja, dok
sastav čvrste frakcije kukuruzne džibre više odgovara nutritivnim potrebama preživara.
PB  - Belgrade : Association of the Chemical Engineers of Serbia
T2  - Chemical Industry and Chemical Engineering Quarterly
T1  - Utilization of stillages from bioethanol production from various substrates
T1  - Mogućnosti upotrebe džibre iz proizvodnje bioetanola na različitim supstratima
VL  - 25
IS  - 2
SP  - 97
EP  - 106
DO  - 10.2298/CICEQ180123023D
ER  - 

@article{
author = "Djukić-Vuković, Aleksandra and Mladenović, Dragana and Nikolić, Valentina and Kocic-Tanackov, Suncica and Pejin, Jelena and Mojović, Ljiljana",
year = "2019",
abstract = "Stillage is a main by-product of the bioethanol industry and, depending on the
origin of substrates for bioethanol production, it can be a significant pollutant
affecting the profitability of bioethanol production. Directing the stillage towards
the production of bio-based chemicals or high-quality feed is a preferred stra-
tegy. In this paper, a complete utilization of stillages of different origins was
assessed. Thin stillages from bioethanol production from molasses, wasted
bread and corn were chemically characterized, evaluated and compared as
substrates for lactic acid (LA) and probiotic biomass production by Lactobacil-
lus rhamnosus ATCC 7469, while solid fractions of wasted bread and corn stil-
lages were analyzed for feed. The impact of pH control using CaCO 3 or NaOH
was also examined, both in terms of LA production and valorization of the
remains generated in each process.A maximal LA productivity of 1.14 g/(L h)
was obtained on thin wasted bread stillage with pH control by NaOH while the
number of viable probiotic bacterial cells was above 109 CFU/mL. The com-
position of the solid fraction of the wasted bread stillage was complementary
with the needs of monogastric animals, while the solid fraction of corn stillage
was more adequate for the nutritional requirements of ruminants., Džibra je najznačajniji otpadni ili sporedni proizvod industrije bioetanola. U zavisnosti od
porekla supstrata koji se koriste u proizvodnji bioetanola, može biti značajan zagađivač,
pa utiče na profitabilnost proizvodnje bioetanola. Upotreba džibre u biotehnološkim
postupcima proizvodnje hemikalija ili visoko vredne stočne hrane je poželjna strategija
za smanjenje negativnih efekata džibre na životnu sredinu i profitabilnost proizvodn je
bioetanola. U ovom radu je analizirana mogućnost kompletnog iskorišćenja džibre.
Tečne džibre iz proizvodnje bioetanola na melasi, otpadnom hlebu i kukuruzu su
hemijski okarakterisane i upoređene kao supstrati za proizvodnju mlečne kiseline (MK) i
probiotske biomase soja Lactobacillus rhamnosus ATCC 7469, dok je čvrsti ostatak
hlebne i kukuruzne džibre analiziran kao hrana za životinje. Takođe je ispitan uticaj pH
kontrole pomoću CaCO3 ili NaOH sa aspekta proizvodnje MK i sa aspekta iskorišćenja
ostataka iz oba procesa. Maksimalna produktivnost MK od 1,14 g/(L h) je dobijena na
tečnoj džibri iz otpadnog hleba sa pH kontorolom pomoću NaOH, dok je broj živih pro-
biotskih bakterija bio preko 10 9 CFU/mL. Rezultati su pokazali da je sastav čvrste frak-
cije otpadne hlebne džibre komplementaran sa potrebama monogastričnih životinja, dok
sastav čvrste frakcije kukuruzne džibre više odgovara nutritivnim potrebama preživara.",
publisher = "Belgrade : Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry and Chemical Engineering Quarterly",
title = "Utilization of stillages from bioethanol production from various substrates, Mogućnosti upotrebe džibre iz proizvodnje bioetanola na različitim supstratima",
volume = "25",
number = "2",
pages = "97-106",
doi = "10.2298/CICEQ180123023D"
}

Djukić-Vuković, A., Mladenović, D., Nikolić, V., Kocic-Tanackov, S., Pejin, J.,& Mojović, L.. (2019). Utilization of stillages from bioethanol production from various substrates. in Chemical Industry and Chemical Engineering Quarterly
Belgrade : Association of the Chemical Engineers of Serbia., 25(2), 97-106.
https://doi.org/10.2298/CICEQ180123023D

Djukić-Vuković A, Mladenović D, Nikolić V, Kocic-Tanackov S, Pejin J, Mojović L. Utilization of stillages from bioethanol production from various substrates. in Chemical Industry and Chemical Engineering Quarterly. 2019;25(2):97-106.
doi:10.2298/CICEQ180123023D .

Djukić-Vuković, Aleksandra, Mladenović, Dragana, Nikolić, Valentina, Kocic-Tanackov, Suncica, Pejin, Jelena, Mojović, Ljiljana, "Utilization of stillages from bioethanol production from various substrates" in Chemical Industry and Chemical Engineering Quarterly, 25, no. 2 (2019):97-106,
https://doi.org/10.2298/CICEQ180123023D . .

TY  - JOUR
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Semenčenko, Valentina
AU  - Radosavljević, Milica
AU  - Pejin, Jelena
AU  - Kocic-Tanackov, Suncica D.
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/584
AB  - Utilization of distillery stillage from bioethanol production for lactic acid and feed production was studied. The lactic acid fermentation of the stillage was performed by Lactobacillus rhamnosus ATCC 7469 and maximal lactic acid concentration of 50.18 g L-1, yield of 0.90 g g(-1), productivity of 1.48 g L-1 h(-1) and viable cell number of 5 x 10(9) CPU mL(-1) were achieved. Solid residues with biomass remains after lactic acid fermentation were assessed for animal consumption. The content of proteins and ash decreased in the residues after the fermentation, whilst the content of oil and nitrogen free extract was higher when compared to unfermented samples. The digestible (17480.64 kJ kg(-1)) and metabolisable (17389.08 kJ kg(-1)) energies as well as digestibility (966.95 g kg(-1)) of the fermentation residue were very high. The in vitro assessment of L. rhamnosus ATCC 7469 survival in simulated gastric conditions has shown high survival rate (87%). In addition, this bacterium has shown good antimicrobial activity against the most important pathogens and capability to produce exopolysaccharide on different sugars present in animal diet. After effective lactic acid fermentation, the residues could be recommended as a high quality feed for monogastric animals.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Effective valorisation of distillery stillage by integrated production of lactic acid and high quality feed
VL  - 73
SP  - 75
EP  - 80
DO  - 10.1016/j.foodres.2014.07.048
ER  - 

@article{
author = "Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Semenčenko, Valentina and Radosavljević, Milica and Pejin, Jelena and Kocic-Tanackov, Suncica D.",
year = "2015",
abstract = "Utilization of distillery stillage from bioethanol production for lactic acid and feed production was studied. The lactic acid fermentation of the stillage was performed by Lactobacillus rhamnosus ATCC 7469 and maximal lactic acid concentration of 50.18 g L-1, yield of 0.90 g g(-1), productivity of 1.48 g L-1 h(-1) and viable cell number of 5 x 10(9) CPU mL(-1) were achieved. Solid residues with biomass remains after lactic acid fermentation were assessed for animal consumption. The content of proteins and ash decreased in the residues after the fermentation, whilst the content of oil and nitrogen free extract was higher when compared to unfermented samples. The digestible (17480.64 kJ kg(-1)) and metabolisable (17389.08 kJ kg(-1)) energies as well as digestibility (966.95 g kg(-1)) of the fermentation residue were very high. The in vitro assessment of L. rhamnosus ATCC 7469 survival in simulated gastric conditions has shown high survival rate (87%). In addition, this bacterium has shown good antimicrobial activity against the most important pathogens and capability to produce exopolysaccharide on different sugars present in animal diet. After effective lactic acid fermentation, the residues could be recommended as a high quality feed for monogastric animals.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Effective valorisation of distillery stillage by integrated production of lactic acid and high quality feed",
volume = "73",
pages = "75-80",
doi = "10.1016/j.foodres.2014.07.048"
}

Đukić-Vuković, A., Mojović, L., Semenčenko, V., Radosavljević, M., Pejin, J.,& Kocic-Tanackov, S. D.. (2015). Effective valorisation of distillery stillage by integrated production of lactic acid and high quality feed. in Food Research International
Elsevier Science Bv, Amsterdam., 73, 75-80.
https://doi.org/10.1016/j.foodres.2014.07.048

Đukić-Vuković A, Mojović L, Semenčenko V, Radosavljević M, Pejin J, Kocic-Tanackov SD. Effective valorisation of distillery stillage by integrated production of lactic acid and high quality feed. in Food Research International. 2015;73:75-80.
doi:10.1016/j.foodres.2014.07.048 .

Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Semenčenko, Valentina, Radosavljević, Milica, Pejin, Jelena, Kocic-Tanackov, Suncica D., "Effective valorisation of distillery stillage by integrated production of lactic acid and high quality feed" in Food Research International, 73 (2015):75-80,
https://doi.org/10.1016/j.foodres.2014.07.048 . .

TY  - JOUR
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Semenčenko, Valentina
AU  - Radosavljević, Milica
AU  - Terzić, Dušanka
AU  - Nikolić, Svetlana
AU  - Pejin, Jelena
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/504
AB  - Lactic acid is a significant chemical for the food industry. Fermentative lactic acid production on wastes could significantly improve the economy and sustainability of the process. In this study, lactic acid production was performed by L. rhamnosus ATCC 7469 on a stillage from bioethanol production on waste bread. Under optimal conditions, in fed-batch fermentation lactic acid productivity of 1.80 g L-1 h-1 has been achieved with a cell number of above 109 CFU mL-1. L. rhamnosus has shown high survival rate of over 85% in the presence of beef bile and at low pH value of MRS broth. The residuals after the fermentation were chemically analyzed and their composition corresponded well with the values recommended for the feed. The digestible energy was 17452.25 kJ kg-1, while metabolisable energy was 17360.83 kJ kg-1. The high values of energy parameters indicated that an integrated process for lactic acid and feedstuff production could be a good strategy.
AB  - Mlečna kiselina je važna supstanca za prehrambenu industriju. Proizvodnja mlečne kiseline fermentacijom na otpadnim sirovinama može značajno da poveća ekonomičnost i održivost procesa. U ovom radu je ispitivana proizvodnja mlečne kiseline pomoću L. rhamnosus ATCC 7469 na džibri iz proizvodnje bioetanola na otpadnom hlebu. Pod optimalnim uslovima, u dolivnom postupku je postignuta produktivnost mlečne kiseline od 1.80 g L-1 h-1 sa više od 109 CFU mL-1 bakterija. L. rhamnosus je pokazao visok stepen preživljavanja od preko 85% u prisustvu žučnih soli i pri niskoj pH vrednosti MRS bujona. Ostatak nakon mlečno-kiselinske fermentacije džibre je hemijski analiziran i pokazano je da dobijene vrednosti odgovaraju preporučenim. Svarljiva energija je iznosila 17452,25 kJ kg-1, dok je metabolička energija bila 17360,83 kJ kg-1. Visoke vrednosti energetskih parametara hraniva ukazuju da integrisani proces proizvodnje mlečne kiseline i stočne hrane na džibri predstavlja povoljnu strategiju.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Evaluation of the residue of lactic acid fermentation on stillage as an animal feed
T1  - Ispitivanje kvaliteta džibre nakon mlečno-kiselinske fermentacije kao hrane za životinje
VL  - 17
IS  - 2
SP  - 64
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_rik_504
ER  - 

@article{
author = "Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Semenčenko, Valentina and Radosavljević, Milica and Terzić, Dušanka and Nikolić, Svetlana and Pejin, Jelena",
year = "2013",
abstract = "Lactic acid is a significant chemical for the food industry. Fermentative lactic acid production on wastes could significantly improve the economy and sustainability of the process. In this study, lactic acid production was performed by L. rhamnosus ATCC 7469 on a stillage from bioethanol production on waste bread. Under optimal conditions, in fed-batch fermentation lactic acid productivity of 1.80 g L-1 h-1 has been achieved with a cell number of above 109 CFU mL-1. L. rhamnosus has shown high survival rate of over 85% in the presence of beef bile and at low pH value of MRS broth. The residuals after the fermentation were chemically analyzed and their composition corresponded well with the values recommended for the feed. The digestible energy was 17452.25 kJ kg-1, while metabolisable energy was 17360.83 kJ kg-1. The high values of energy parameters indicated that an integrated process for lactic acid and feedstuff production could be a good strategy., Mlečna kiselina je važna supstanca za prehrambenu industriju. Proizvodnja mlečne kiseline fermentacijom na otpadnim sirovinama može značajno da poveća ekonomičnost i održivost procesa. U ovom radu je ispitivana proizvodnja mlečne kiseline pomoću L. rhamnosus ATCC 7469 na džibri iz proizvodnje bioetanola na otpadnom hlebu. Pod optimalnim uslovima, u dolivnom postupku je postignuta produktivnost mlečne kiseline od 1.80 g L-1 h-1 sa više od 109 CFU mL-1 bakterija. L. rhamnosus je pokazao visok stepen preživljavanja od preko 85% u prisustvu žučnih soli i pri niskoj pH vrednosti MRS bujona. Ostatak nakon mlečno-kiselinske fermentacije džibre je hemijski analiziran i pokazano je da dobijene vrednosti odgovaraju preporučenim. Svarljiva energija je iznosila 17452,25 kJ kg-1, dok je metabolička energija bila 17360,83 kJ kg-1. Visoke vrednosti energetskih parametara hraniva ukazuju da integrisani proces proizvodnje mlečne kiseline i stočne hrane na džibri predstavlja povoljnu strategiju.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Evaluation of the residue of lactic acid fermentation on stillage as an animal feed, Ispitivanje kvaliteta džibre nakon mlečno-kiselinske fermentacije kao hrane za životinje",
volume = "17",
number = "2",
pages = "64-67",
url = "https://hdl.handle.net/21.15107/rcub_rik_504"
}

Đukić-Vuković, A., Mojović, L., Semenčenko, V., Radosavljević, M., Terzić, D., Nikolić, S.,& Pejin, J.. (2013). Evaluation of the residue of lactic acid fermentation on stillage as an animal feed. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 17(2), 64-67.
https://hdl.handle.net/21.15107/rcub_rik_504

Đukić-Vuković A, Mojović L, Semenčenko V, Radosavljević M, Terzić D, Nikolić S, Pejin J. Evaluation of the residue of lactic acid fermentation on stillage as an animal feed. in Journal on Processing and Energy in Agriculture. 2013;17(2):64-67.
https://hdl.handle.net/21.15107/rcub_rik_504 .

Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Semenčenko, Valentina, Radosavljević, Milica, Terzić, Dušanka, Nikolić, Svetlana, Pejin, Jelena, "Evaluation of the residue of lactic acid fermentation on stillage as an animal feed" in Journal on Processing and Energy in Agriculture, 17, no. 2 (2013):64-67,
https://hdl.handle.net/21.15107/rcub_rik_504 .

TY  - CHAP
AU  - Mojović, Ljiljana
AU  - Nikolić, Svetlana
AU  - Pejin, Dušanka
AU  - Djukić-Vuković, Aleksandra
AU  - Kocic-Tanackov, Suncica
AU  - Nikolić, Valentina
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/868
AB  - Bioethanol has become one of the most promising biofuels today in response to uncertain fuel supplies and efforts to
reduce greenhouse gas emissions. The biofuels are easily available from common biomass sources, biodegradable and
contribute to sustainability. In Serbia, the industrial production of bioethanol still relies on conventional energy crops
containing starch and sugar such as corn, wheat and triticale, which are the most suitable and available agricultural raw
materials. The preview of bioethanol production possibilities and available feedstocks in Serbia are presented in this study.
Several production approaches based on crop selection, process integration and waste utilization were also considered in
order to increase production efficiency and to avoid the competition of the feedstock utilization for food and energy.
Utilization of corn, wheat and triticale (plant resistant to severe climate and soil conditions) were investigated for
bioethanol production as well as utilization of damaged crops (e.g. wheat) that are not appropriate for food consumption.
Also, utilization of the stillage for the production of lactic acid could also improve the bioethanol production. The
economy of bioethanol production was analyzed in order to decrease the production costs and make this biofuel
competitive with fossil fuels. The analysis has compared the cost of bioethanol produced from three crops that can be
cultivated in Serbia: corn, wheat, and triticale, and the triticale has shown as the most favourable.
PB  - Badajoz : Formatex Research Center
T2  - Materials and processes for energy: communicating current research and technological developments
T1  - The potential for sustainable bioethanol production in Serbia: available biomass and new production approaches
SP  - 380
EP  - 392
UR  - https://hdl.handle.net/21.15107/rcub_rik_868
ER  - 

@inbook{
author = "Mojović, Ljiljana and Nikolić, Svetlana and Pejin, Dušanka and Djukić-Vuković, Aleksandra and Kocic-Tanackov, Suncica and Nikolić, Valentina",
year = "2013",
abstract = "Bioethanol has become one of the most promising biofuels today in response to uncertain fuel supplies and efforts to
reduce greenhouse gas emissions. The biofuels are easily available from common biomass sources, biodegradable and
contribute to sustainability. In Serbia, the industrial production of bioethanol still relies on conventional energy crops
containing starch and sugar such as corn, wheat and triticale, which are the most suitable and available agricultural raw
materials. The preview of bioethanol production possibilities and available feedstocks in Serbia are presented in this study.
Several production approaches based on crop selection, process integration and waste utilization were also considered in
order to increase production efficiency and to avoid the competition of the feedstock utilization for food and energy.
Utilization of corn, wheat and triticale (plant resistant to severe climate and soil conditions) were investigated for
bioethanol production as well as utilization of damaged crops (e.g. wheat) that are not appropriate for food consumption.
Also, utilization of the stillage for the production of lactic acid could also improve the bioethanol production. The
economy of bioethanol production was analyzed in order to decrease the production costs and make this biofuel
competitive with fossil fuels. The analysis has compared the cost of bioethanol produced from three crops that can be
cultivated in Serbia: corn, wheat, and triticale, and the triticale has shown as the most favourable.",
publisher = "Badajoz : Formatex Research Center",
journal = "Materials and processes for energy: communicating current research and technological developments",
booktitle = "The potential for sustainable bioethanol production in Serbia: available biomass and new production approaches",
pages = "380-392",
url = "https://hdl.handle.net/21.15107/rcub_rik_868"
}

Mojović, L., Nikolić, S., Pejin, D., Djukić-Vuković, A., Kocic-Tanackov, S.,& Nikolić, V.. (2013). The potential for sustainable bioethanol production in Serbia: available biomass and new production approaches. in Materials and processes for energy: communicating current research and technological developments
Badajoz : Formatex Research Center., 380-392.
https://hdl.handle.net/21.15107/rcub_rik_868

Mojović L, Nikolić S, Pejin D, Djukić-Vuković A, Kocic-Tanackov S, Nikolić V. The potential for sustainable bioethanol production in Serbia: available biomass and new production approaches. in Materials and processes for energy: communicating current research and technological developments. 2013;:380-392.
https://hdl.handle.net/21.15107/rcub_rik_868 .

Mojović, Ljiljana, Nikolić, Svetlana, Pejin, Dušanka, Djukić-Vuković, Aleksandra, Kocic-Tanackov, Suncica, Nikolić, Valentina, "The potential for sustainable bioethanol production in Serbia: available biomass and new production approaches" in Materials and processes for energy: communicating current research and technological developments (2013):380-392,
https://hdl.handle.net/21.15107/rcub_rik_868 .