Vuletić, Mirjana

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  • Vuletić, Mirjana (10)
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Author's Bibliography

Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Vuletić, Mirjana; Marković, Ksenija; Kravić, Natalija

(Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid, 2016)

TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
AU  - Kravić, Natalija
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/618
AB  - Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.
PB  - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
T2  - Spanish Journal of Agricultural Research
T1  - Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources
VL  - 14
IS  - 4
DO  - 10.5424/sjar/2016144-8305
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Vuletić, Mirjana and Marković, Ksenija and Kravić, Natalija",
year = "2016",
url = "http://rik.mrizp.rs/handle/123456789/618",
abstract = "Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.",
publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid",
journal = "Spanish Journal of Agricultural Research",
title = "Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources",
volume = "14",
number = "4",
doi = "10.5424/sjar/2016144-8305"
}
Hadži-Tašković-Šukalović, V., Vučinić, Ž., Vuletić, M., Marković, K.,& Kravić, N. (2016). Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources.
Spanish Journal of Agricultural Research
Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 14(4).
https://doi.org/10.5424/sjar/2016144-8305
Hadži-Tašković-Šukalović V, Vučinić Ž, Vuletić M, Marković K, Kravić N. Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources. Spanish Journal of Agricultural Research. 2016;14(4)
Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Vuletić Mirjana, Marković Ksenija, Kravić Natalija, "Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources" Spanish Journal of Agricultural Research, 14, no. 4 (2016),
https://doi.org/10.5424/sjar/2016144-8305 .
2
3

Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding

Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Vuletić, Mirjana; Marković, Ksenija; Cvetic-Antić, Tijana

(Springer Wien, Wien, 2015)

TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
AU  - Cvetic-Antić, Tijana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/591
AB  - Comparative biochemical characterization of class III peroxidase activity tightly bound to the cell walls of maize roots was performed. Ionically bound proteins were solubilized from isolated walls by salt washing, and the remaining covalently bound peroxidases were released, either by enzymatic digestion or by a novel alkaline extraction procedure that released covalently bound alkali-resistant peroxidase enzyme. Solubilized fractions, as well as the salt-washed cell wall fragments containing covalently bound proteins, were analyzed for peroxidase activity. Peroxidative and oxidative activities indicated that peroxidase enzymes were predominately associated with walls by ionic interactions, and this fraction differs from the covalently bound one according to molecular weight, isozyme patterns, and biochemical parameters. The effect of covalent binding was evaluated by comparison of the catalytic properties of the enzyme bound to the salt-washed cell wall fragments with the corresponding solubilized and released enzyme. Higher thermal stability, improved resistance to KCN, increased susceptibility to H2O2, stimulated capacity of wall-bound enzyme to oxidize indole-3-acetic acid (IAA) as well as the difference in kinetic parameters between free and bound enzymes point to conformational changes due to covalent binding. Differences in biochemical properties of ionically and covalently bound peroxidases, as well as the modulation of the enzyme properties as a result of covalent binding to the walls, indicate that these two fractions of apoplastic peroxidases play different roles.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding
VL  - 252
IS  - 1
SP  - 335
EP  - 343
DO  - 10.1007/s00709-014-0684-2
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Vuletić, Mirjana and Marković, Ksenija and Cvetic-Antić, Tijana",
year = "2015",
url = "http://rik.mrizp.rs/handle/123456789/591",
abstract = "Comparative biochemical characterization of class III peroxidase activity tightly bound to the cell walls of maize roots was performed. Ionically bound proteins were solubilized from isolated walls by salt washing, and the remaining covalently bound peroxidases were released, either by enzymatic digestion or by a novel alkaline extraction procedure that released covalently bound alkali-resistant peroxidase enzyme. Solubilized fractions, as well as the salt-washed cell wall fragments containing covalently bound proteins, were analyzed for peroxidase activity. Peroxidative and oxidative activities indicated that peroxidase enzymes were predominately associated with walls by ionic interactions, and this fraction differs from the covalently bound one according to molecular weight, isozyme patterns, and biochemical parameters. The effect of covalent binding was evaluated by comparison of the catalytic properties of the enzyme bound to the salt-washed cell wall fragments with the corresponding solubilized and released enzyme. Higher thermal stability, improved resistance to KCN, increased susceptibility to H2O2, stimulated capacity of wall-bound enzyme to oxidize indole-3-acetic acid (IAA) as well as the difference in kinetic parameters between free and bound enzymes point to conformational changes due to covalent binding. Differences in biochemical properties of ionically and covalently bound peroxidases, as well as the modulation of the enzyme properties as a result of covalent binding to the walls, indicate that these two fractions of apoplastic peroxidases play different roles.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding",
volume = "252",
number = "1",
pages = "335-343",
doi = "10.1007/s00709-014-0684-2"
}
Hadži-Tašković-Šukalović, V., Vučinić, Ž., Vuletić, M., Marković, K.,& Cvetic-Antić, T. (2015). Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding.
Protoplasma
Springer Wien, Wien., 252(1), 335-343.
https://doi.org/10.1007/s00709-014-0684-2
Hadži-Tašković-Šukalović V, Vučinić Ž, Vuletić M, Marković K, Cvetic-Antić T. Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding. Protoplasma. 2015;252(1):335-343
Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Vuletić Mirjana, Marković Ksenija, Cvetic-Antić Tijana, "Comparative biochemical characterization of peroxidases (class III) tightly bound to the maize root cell walls and modulation of the enzyme properties as a result of covalent binding" Protoplasma, 252, no. 1 (2015):335-343,
https://doi.org/10.1007/s00709-014-0684-2 .
1
6
4
6

Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress

Vuletić, Mirjana; Marković, Ksenija; Kravić, Natalija; Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Maksimović, Vuk

(Wiley-Blackwell, Hoboken, 2014)

TY  - JOUR
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
AU  - Kravić, Natalija
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/551
AB  - An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Biology
T1  - Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress
VL  - 16
IS  - 1
SP  - 88
EP  - 96
DO  - 10.1111/plb.12017
ER  - 
@article{
author = "Vuletić, Mirjana and Marković, Ksenija and Kravić, Natalija and Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Maksimović, Vuk",
year = "2014",
url = "http://rik.mrizp.rs/handle/123456789/551",
abstract = "An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Biology",
title = "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress",
volume = "16",
number = "1",
pages = "88-96",
doi = "10.1111/plb.12017"
}
Vuletić, M., Marković, K., Kravić, N., Hadži-Tašković-Šukalović, V., Vučinić, Ž.,& Maksimović, V. (2014). Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress.
Plant Biology
Wiley-Blackwell, Hoboken., 16(1), 88-96.
https://doi.org/10.1111/plb.12017
Vuletić M, Marković K, Kravić N, Hadži-Tašković-Šukalović V, Vučinić Ž, Maksimović V. Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress. Plant Biology. 2014;16(1):88-96
Vuletić Mirjana, Marković Ksenija, Kravić Natalija, Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Maksimović Vuk, "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress" Plant Biology, 16, no. 1 (2014):88-96,
https://doi.org/10.1111/plb.12017 .
15
16
19

Growth, proline accumulation and peroxidase activity in maize seedlings under osmotic stress

Kravić, Natalija; Marković, Ksenija; Anđelković, Violeta; Babić, Vojka; Vuletić, Mirjana; Hadži-Tašković-Šukalović, Vesna

(Springer Heidelberg, Heidelberg, 2013)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Marković, Ksenija
AU  - Anđelković, Violeta
AU  - Babić, Vojka
AU  - Vuletić, Mirjana
AU  - Hadži-Tašković-Šukalović, Vesna
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/491
AB  - The influence of osmotic stress induced by polyethylene glycol (PEG) on plant growth, proline content and activities of soluble peroxidases was studied on 12 maize inbred lines at seedling stage. Reduction of plant growth, fresh weight and length of roots and shoots occurred in all of the studied genotypes and was followed by increase in free proline content of shoots and especially in roots of the majority of genotypes. Correlation analysis of changes in root proline content with growth parameters revealed direct positive correlation. Changes in root peroxidase activities ranged from approximately 40 % reduction to 20 % stimulation, depending on the genotype. It was shown that genotypes with higher proline changes under drought treatment exhibited lower peroxidase activities. In addition, genotypes with less pronounced root growth reduction under stress conditions exhibited increased peroxidase activities, as well as lower proline content. In the field experiments, grain yield was positively correlated with root proline content and negatively with root length changes in drought-treated seedlings grown in laboratory conditions.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Growth, proline accumulation and peroxidase activity in maize seedlings under osmotic stress
VL  - 35
IS  - 1
SP  - 233
EP  - 239
DO  - 10.1007/s11738-012-1068-x
ER  - 
@article{
author = "Kravić, Natalija and Marković, Ksenija and Anđelković, Violeta and Babić, Vojka and Vuletić, Mirjana and Hadži-Tašković-Šukalović, Vesna",
year = "2013",
url = "http://rik.mrizp.rs/handle/123456789/491",
abstract = "The influence of osmotic stress induced by polyethylene glycol (PEG) on plant growth, proline content and activities of soluble peroxidases was studied on 12 maize inbred lines at seedling stage. Reduction of plant growth, fresh weight and length of roots and shoots occurred in all of the studied genotypes and was followed by increase in free proline content of shoots and especially in roots of the majority of genotypes. Correlation analysis of changes in root proline content with growth parameters revealed direct positive correlation. Changes in root peroxidase activities ranged from approximately 40 % reduction to 20 % stimulation, depending on the genotype. It was shown that genotypes with higher proline changes under drought treatment exhibited lower peroxidase activities. In addition, genotypes with less pronounced root growth reduction under stress conditions exhibited increased peroxidase activities, as well as lower proline content. In the field experiments, grain yield was positively correlated with root proline content and negatively with root length changes in drought-treated seedlings grown in laboratory conditions.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Growth, proline accumulation and peroxidase activity in maize seedlings under osmotic stress",
volume = "35",
number = "1",
pages = "233-239",
doi = "10.1007/s11738-012-1068-x"
}
Kravić, N., Marković, K., Anđelković, V., Babić, V., Vuletić, M.,& Hadži-Tašković-Šukalović, V. (2013). Growth, proline accumulation and peroxidase activity in maize seedlings under osmotic stress.
Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 35(1), 233-239.
https://doi.org/10.1007/s11738-012-1068-x
Kravić N, Marković K, Anđelković V, Babić V, Vuletić M, Hadži-Tašković-Šukalović V. Growth, proline accumulation and peroxidase activity in maize seedlings under osmotic stress. Acta Physiologiae Plantarum. 2013;35(1):233-239
Kravić Natalija, Marković Ksenija, Anđelković Violeta, Babić Vojka, Vuletić Mirjana, Hadži-Tašković-Šukalović Vesna, "Growth, proline accumulation and peroxidase activity in maize seedlings under osmotic stress" Acta Physiologiae Plantarum, 35, no. 1 (2013):233-239,
https://doi.org/10.1007/s11738-012-1068-x .
14
14
13

Morpho-physiological changes in maize seedling sunder osmotic stress

Anđelković, Violeta; Vuletić, Mirjana; Kravić, Natalija; Filipović, Milomir; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2012)

TY  - JOUR
AU  - Anđelković, Violeta
AU  - Vuletić, Mirjana
AU  - Kravić, Natalija
AU  - Filipović, Milomir
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/461
AB  - Drought is a major abiotic stress factor limiting crop growth, development and production worldwide. The objective of this study was to evaluate tolerance to osmotic stress of maize seedlings. More than 6,000 accessions from the Maize Research Institute gene bank were tested under controlled drought (at flowering) in Egypt, and afterwards in temperate climate (Serbia and Macedonia). Out of 41 drought tolerant accessions in the field, five inbred lines were chosen for laboratory testing, as well as one drought sensitive line. These genotypes were exposed to 4% polyethylene glycol-PEG (Mr 10000) for 24 h and 48 h. Nine-day-old seedlings compared to control conditions were analyzed in root and shoot length, fresh and dry weight and proline content. Results showed reduction in all parameters under stress, while only proline content increased in all PEG treated genotypes compared to control.
AB  - Suša je glavni abiotički stres koji ograničava porast, razvoj i produktivnost useva širom sveta. Cilj ovog rada bio je ocena tolerantnosti klijanaca kukuruza na osmotski stres. Više od 6.000 uzoraka banke gena Instituta za kukuruz testirano je u uslovima kontrolisanog stresa suše (faza cvetanja) u Egiptu, a posle toga u umerenom klimatu (Srbija i Makedonija). Od 41 uzorka tolerantnog na sušu u poljskim uslovima, odabrano je pet linija, kao i jedna osetljiva na sušu, za testiranje u laboratoriji. Ovi genotipovi su izloženi 4% polietilen glikolu (PEG, Mr 10000) u trajanju od 24 h i 48 h. Odgovor klijanaca izloženih stresu (starih 9 dana) u odnosu na kontrolu, analiziran je kroz porast korena i izdanka, svežu i suvu masu i sadržaj prolina. Rezultati su pokazali smanjenje svih parametara u uslovima stresa, dok je jedino sadržaj prolina bio u porastu kod svih ispitivanih genotipova pod uticajem PEG-a u odnosu na kontrolu.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Morpho-physiological changes in maize seedling sunder osmotic stress
T1  - Morfološko-fiziološke promene u klijancima kukuruza u uslovima osmotskog stresa
VL  - 49
IS  - 3
SP  - 263
EP  - 269
DO  - 10.5937/ratpov49-2485
ER  - 
@article{
author = "Anđelković, Violeta and Vuletić, Mirjana and Kravić, Natalija and Filipović, Milomir and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2012",
url = "http://rik.mrizp.rs/handle/123456789/461",
abstract = "Drought is a major abiotic stress factor limiting crop growth, development and production worldwide. The objective of this study was to evaluate tolerance to osmotic stress of maize seedlings. More than 6,000 accessions from the Maize Research Institute gene bank were tested under controlled drought (at flowering) in Egypt, and afterwards in temperate climate (Serbia and Macedonia). Out of 41 drought tolerant accessions in the field, five inbred lines were chosen for laboratory testing, as well as one drought sensitive line. These genotypes were exposed to 4% polyethylene glycol-PEG (Mr 10000) for 24 h and 48 h. Nine-day-old seedlings compared to control conditions were analyzed in root and shoot length, fresh and dry weight and proline content. Results showed reduction in all parameters under stress, while only proline content increased in all PEG treated genotypes compared to control., Suša je glavni abiotički stres koji ograničava porast, razvoj i produktivnost useva širom sveta. Cilj ovog rada bio je ocena tolerantnosti klijanaca kukuruza na osmotski stres. Više od 6.000 uzoraka banke gena Instituta za kukuruz testirano je u uslovima kontrolisanog stresa suše (faza cvetanja) u Egiptu, a posle toga u umerenom klimatu (Srbija i Makedonija). Od 41 uzorka tolerantnog na sušu u poljskim uslovima, odabrano je pet linija, kao i jedna osetljiva na sušu, za testiranje u laboratoriji. Ovi genotipovi su izloženi 4% polietilen glikolu (PEG, Mr 10000) u trajanju od 24 h i 48 h. Odgovor klijanaca izloženih stresu (starih 9 dana) u odnosu na kontrolu, analiziran je kroz porast korena i izdanka, svežu i suvu masu i sadržaj prolina. Rezultati su pokazali smanjenje svih parametara u uslovima stresa, dok je jedino sadržaj prolina bio u porastu kod svih ispitivanih genotipova pod uticajem PEG-a u odnosu na kontrolu.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Morpho-physiological changes in maize seedling sunder osmotic stress, Morfološko-fiziološke promene u klijancima kukuruza u uslovima osmotskog stresa",
volume = "49",
number = "3",
pages = "263-269",
doi = "10.5937/ratpov49-2485"
}
Anđelković, V., Vuletić, M., Kravić, N., Filipović, M., Ignjatović-Micić, D.,& Vančetović, J. (2012). Morfološko-fiziološke promene u klijancima kukuruza u uslovima osmotskog stresa.
Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(3), 263-269.
https://doi.org/10.5937/ratpov49-2485
Anđelković V, Vuletić M, Kravić N, Filipović M, Ignjatović-Micić D, Vančetović J. Morfološko-fiziološke promene u klijancima kukuruza u uslovima osmotskog stresa. Ratarstvo i povrtarstvo. 2012;49(3):263-269
Anđelković Violeta, Vuletić Mirjana, Kravić Natalija, Filipović Milomir, Ignjatović-Micić Dragana, Vančetović Jelena, "Morfološko-fiziološke promene u klijancima kukuruza u uslovima osmotskog stresa" Ratarstvo i povrtarstvo, 49, no. 3 (2012):263-269,
https://doi.org/10.5937/ratpov49-2485 .

Cell wall-associated malate dehydrogenase activity from maize roots

Hadži-Tašković-Šukalović, Vesna; Vučinić, Željko; Vuletić, Mirjana; Marković, Ksenija

(Elsevier Ireland Ltd, Clare, 2011)

TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vučinić, Željko
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
PY  - 2011
UR  - http://rik.mrizp.rs/handle/123456789/373
AB  - Isolated cell walls from maize (Zea mays L) roots exhibited ionically and covalently bound NAD-specific malate dehydrogenase activity. The enzyme catalyses a rapid reduction of oxaloacetate and much slower oxidation of malate. The kinetic and regulatory properties of the cell wall enzyme solubilized with 1 M NaCl were different from those published for soluble, mitochondrial or plasma membrane malate dehydrogenase with respect to their ATP, Pi, and pH dependence. Isoelectric focusing of ionically-bound proteins and specific staining for malate dehydrogenase revealed characteristic isoforms present in cell wall isolate, different from those present in plasma membranes and crude homogenate. Much greater activity of cell wall-associated malate dehydrogenase was detected in the intensively growing lateral roots compared to primary root with decreased growth rates. Presence of Zn2+ and Cu2+ in the assay medium inhibited the activity of the wall-associated malate dehydrogenase. Exposure of maize plants to excess concentrations of Zn2+ and Cu2+ in the hydroponic solution inhibited lateral root growth, decreased malate dehydrogenase activity and changed isoform profiles. The results presented show that cell wall malate dehydrogenase is truly a wall-bound enzyme, and not an artefact of cytoplasmic contamination, involved in the developmental processes, and detoxification of heavy metals.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Cell wall-associated malate dehydrogenase activity from maize roots
VL  - 181
IS  - 4
SP  - 465
EP  - 470
DO  - 10.1016/j.plantsci.2011.07.007
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vučinić, Željko and Vuletić, Mirjana and Marković, Ksenija",
year = "2011",
url = "http://rik.mrizp.rs/handle/123456789/373",
abstract = "Isolated cell walls from maize (Zea mays L) roots exhibited ionically and covalently bound NAD-specific malate dehydrogenase activity. The enzyme catalyses a rapid reduction of oxaloacetate and much slower oxidation of malate. The kinetic and regulatory properties of the cell wall enzyme solubilized with 1 M NaCl were different from those published for soluble, mitochondrial or plasma membrane malate dehydrogenase with respect to their ATP, Pi, and pH dependence. Isoelectric focusing of ionically-bound proteins and specific staining for malate dehydrogenase revealed characteristic isoforms present in cell wall isolate, different from those present in plasma membranes and crude homogenate. Much greater activity of cell wall-associated malate dehydrogenase was detected in the intensively growing lateral roots compared to primary root with decreased growth rates. Presence of Zn2+ and Cu2+ in the assay medium inhibited the activity of the wall-associated malate dehydrogenase. Exposure of maize plants to excess concentrations of Zn2+ and Cu2+ in the hydroponic solution inhibited lateral root growth, decreased malate dehydrogenase activity and changed isoform profiles. The results presented show that cell wall malate dehydrogenase is truly a wall-bound enzyme, and not an artefact of cytoplasmic contamination, involved in the developmental processes, and detoxification of heavy metals.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Cell wall-associated malate dehydrogenase activity from maize roots",
volume = "181",
number = "4",
pages = "465-470",
doi = "10.1016/j.plantsci.2011.07.007"
}
Hadži-Tašković-Šukalović, V., Vučinić, Ž., Vuletić, M.,& Marković, K. (2011). Cell wall-associated malate dehydrogenase activity from maize roots.
Plant Science
Elsevier Ireland Ltd, Clare., 181(4), 465-470.
https://doi.org/10.1016/j.plantsci.2011.07.007
Hadži-Tašković-Šukalović V, Vučinić Ž, Vuletić M, Marković K. Cell wall-associated malate dehydrogenase activity from maize roots. Plant Science. 2011;181(4):465-470
Hadži-Tašković-Šukalović Vesna, Vučinić Željko, Vuletić Mirjana, Marković Ksenija, "Cell wall-associated malate dehydrogenase activity from maize roots" Plant Science, 181, no. 4 (2011):465-470,
https://doi.org/10.1016/j.plantsci.2011.07.007 .
19
23
22

Maize roots responses to osmotic stress

Kravić, Natalija; Vuletić, Mirjana; Anđelković, Violeta

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2010)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Vuletić, Mirjana
AU  - Anđelković, Violeta
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/305
AB  - Field testing for drought tolerance was performed on 6,000 accessions from the Maize Research Institute gene bank, under severe drought conditions in Egypt, as well as, under moderate climate conditions in Zemun Polje and Skopje. Five inbred lines, considered as drought tolerant, were chosen for further investigations under controlled experimental conditions. Osmotic stress caused by drought is one of the most important abiotic stresses. In this study osmotic stress was applied with polyethylene glycol (PEG) 10 000. The response to the PEG treatment of these genotypes was analysed in respect to their root morphology, root length, root fresh and dry weight, proline content and peroxidase activity. Results showed that the root development was less in all genotypes under the PEG treatment. The proline content increased, while the peroxidase activity declined in PEG-treated plants.
AB  - Suša je jedan od najznačajnijih abiotičkih faktora, koji značajno utiče na smanjenje konačnog prinosa zrna kod kukuruza. Predviđanja globalnih klimatskih promena za XXI vek idu u pravcu povećanja temperature vazduha, veće evapotranspiracije i učestalije pojave suše. Za selekciju kukuruza danas, kao i u doglednoj budućnosti, izvori poželjnih svojstava koji se nalaze u bankama gena biće od velikog značaja. Materijal koji se čuva u banci gena Instituta za kukuruz, po obimu i sadržaju, pruža velike mogućnosti. Na osnovu ispitivanja tolerantnosti na sušu kod 6.000 uzoraka iz banke gena Instituta za kukuruz u poljskim uslovima umerenog (Zemun Polje i Skoplje) i aridnog klimata (Egipat), odabrano je pet inbred linija, okarakterisanih kao tolerantne, za dalja ispitivanja u kontrolisanim laboratorijskim uslovima. Linije pripadaju različitim FAO grupama zrenja (A5 - FAO 400 i A1, A2, A3 i A4 - FAO 500). Osmotski stres izazvan sušom je jedan od najznačajnijih abiotičkih faktora. U ovom radu je ispitivan efekat polietilen glikola (PEG) 10.000, kao osmotikuma, na rast korena klijanaca. Merena je dužina, sveža i suva masa korena, određivan je sadržaj prolina u korenu i merena je aktivnost antioksidativnih enzima. Rezultati ispitivanja pokazuju smanjenje porasta korenovog sistema, povećanje sadržaja prolina, kao i smanjenje specifične aktivnosti peroksidaza kod svih tretmana u odnosu na kontrolnu varijantu, kao odgovor na primenjeni osmotski stres. Naredna istraživanja vezana za odgovor biljke na osmotski stres bi uključila i nadzemni deo klijanaca, kao i molekularnu karakterizaciju genotipova tolerantnih prema suši. Odabrani genotipovi poslužiće za formiranje core kolekcije koja će u sebi sadržati ovo svojstvo i biti osnova u programima oplemenjivanja kukuruza.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - Maize roots responses to osmotic stress
T1  - Odgovor korenovog sistema klijanaca kukuruza na osmotski stres
VL  - 71
IS  - 3
SP  - 57
EP  - 65
ER  - 
@article{
author = "Kravić, Natalija and Vuletić, Mirjana and Anđelković, Violeta",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/305",
abstract = "Field testing for drought tolerance was performed on 6,000 accessions from the Maize Research Institute gene bank, under severe drought conditions in Egypt, as well as, under moderate climate conditions in Zemun Polje and Skopje. Five inbred lines, considered as drought tolerant, were chosen for further investigations under controlled experimental conditions. Osmotic stress caused by drought is one of the most important abiotic stresses. In this study osmotic stress was applied with polyethylene glycol (PEG) 10 000. The response to the PEG treatment of these genotypes was analysed in respect to their root morphology, root length, root fresh and dry weight, proline content and peroxidase activity. Results showed that the root development was less in all genotypes under the PEG treatment. The proline content increased, while the peroxidase activity declined in PEG-treated plants., Suša je jedan od najznačajnijih abiotičkih faktora, koji značajno utiče na smanjenje konačnog prinosa zrna kod kukuruza. Predviđanja globalnih klimatskih promena za XXI vek idu u pravcu povećanja temperature vazduha, veće evapotranspiracije i učestalije pojave suše. Za selekciju kukuruza danas, kao i u doglednoj budućnosti, izvori poželjnih svojstava koji se nalaze u bankama gena biće od velikog značaja. Materijal koji se čuva u banci gena Instituta za kukuruz, po obimu i sadržaju, pruža velike mogućnosti. Na osnovu ispitivanja tolerantnosti na sušu kod 6.000 uzoraka iz banke gena Instituta za kukuruz u poljskim uslovima umerenog (Zemun Polje i Skoplje) i aridnog klimata (Egipat), odabrano je pet inbred linija, okarakterisanih kao tolerantne, za dalja ispitivanja u kontrolisanim laboratorijskim uslovima. Linije pripadaju različitim FAO grupama zrenja (A5 - FAO 400 i A1, A2, A3 i A4 - FAO 500). Osmotski stres izazvan sušom je jedan od najznačajnijih abiotičkih faktora. U ovom radu je ispitivan efekat polietilen glikola (PEG) 10.000, kao osmotikuma, na rast korena klijanaca. Merena je dužina, sveža i suva masa korena, određivan je sadržaj prolina u korenu i merena je aktivnost antioksidativnih enzima. Rezultati ispitivanja pokazuju smanjenje porasta korenovog sistema, povećanje sadržaja prolina, kao i smanjenje specifične aktivnosti peroksidaza kod svih tretmana u odnosu na kontrolnu varijantu, kao odgovor na primenjeni osmotski stres. Naredna istraživanja vezana za odgovor biljke na osmotski stres bi uključila i nadzemni deo klijanaca, kao i molekularnu karakterizaciju genotipova tolerantnih prema suši. Odabrani genotipovi poslužiće za formiranje core kolekcije koja će u sebi sadržati ovo svojstvo i biti osnova u programima oplemenjivanja kukuruza.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "Maize roots responses to osmotic stress, Odgovor korenovog sistema klijanaca kukuruza na osmotski stres",
volume = "71",
number = "3",
pages = "57-65"
}
Kravić, N., Vuletić, M.,& Anđelković, V. (2010). Odgovor korenovog sistema klijanaca kukuruza na osmotski stres.
Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 71(3), 57-65.
Kravić N, Vuletić M, Anđelković V. Odgovor korenovog sistema klijanaca kukuruza na osmotski stres. Journal of Scientific Agricultural Research. 2010;71(3):57-65
Kravić Natalija, Vuletić Mirjana, Anđelković Violeta, "Odgovor korenovog sistema klijanaca kukuruza na osmotski stres" Journal of Scientific Agricultural Research, 71, no. 3 (2010):57-65

Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources

Vuletić, Mirjana; Marković, Ksenija; Hadži-Tašković-Šukalović, Vesna; Dragisic-Maksimović, Jelena

(Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6, 2010)

TY  - JOUR
AU  - Vuletić, Mirjana
AU  - Marković, Ksenija
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Dragisic-Maksimović, Jelena
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/351
AB  - The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.
PB  - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
T2  - Biologia Plantarum
T1  - Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
VL  - 54
IS  - 3
SP  - 530
EP  - 534
DO  - 10.1007/s10535-010-0093-0
ER  - 
@article{
author = "Vuletić, Mirjana and Marković, Ksenija and Hadži-Tašković-Šukalović, Vesna and Dragisic-Maksimović, Jelena",
year = "2010",
url = "http://rik.mrizp.rs/handle/123456789/351",
abstract = "The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 (-)) or both nitrogen forms, nitrate and ammonium (NH4 (+)/NO3 (-)). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 (+)/NO3 (-) in comparison with NO3 (-) grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 (+)/NO3 (-) treatment. The PEG treatment decreased enzyme activities in NH4 (+)/NO3 (-) grown plants, but in NO3 (-) grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 (-) grown plants, and lower activities of both, acidic and basic isoforms in NH4 (+)/NO3 (-)grown plants.",
publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6",
journal = "Biologia Plantarum",
title = "Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources",
volume = "54",
number = "3",
pages = "530-534",
doi = "10.1007/s10535-010-0093-0"
}
Vuletić, M., Marković, K., Hadži-Tašković-Šukalović, V.,& Dragisic-Maksimović, J. (2010). Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources.
Biologia Plantarum
Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 54(3), 530-534.
https://doi.org/10.1007/s10535-010-0093-0
Vuletić M, Marković K, Hadži-Tašković-Šukalović V, Dragisic-Maksimović J. Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources. Biologia Plantarum. 2010;54(3):530-534
Vuletić Mirjana, Marković Ksenija, Hadži-Tašković-Šukalović Vesna, Dragisic-Maksimović Jelena, "Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources" Biologia Plantarum, 54, no. 3 (2010):530-534,
https://doi.org/10.1007/s10535-010-0093-0 .
13
13
14

Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm

Kravić, Natalija; Anđelković, Violeta; Hadži-Tašković-Šukalović, Vesna; Vuletić, Mirjana

(Društvo genetičara Srbije, Beograd, 2009)

TY  - JOUR
AU  - Kravić, Natalija
AU  - Anđelković, Violeta
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vuletić, Mirjana
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/290
AB  - In order to broaden the genetic base of maize (Zea mays L.) germplasm, it is necessary to integrate exotic materials into adapted breeding materials. The aim of the study was to compare antioxidative systems of two adapted maize inbred lines (A and B) with exotic germplasm, Drought Tolerant Population (DTP), and their backcrosses with DTP (A1, A2 and B1, B2). The content of low-molecular weight antioxidants, proline and phenolics, as well as antioxidant capacity, detected as free radical scavenging activities against DPPH radical, were measured in maize seeds. Proline content in both, embryo and endosperm was higher in backcrosses than in inbred lines and DTP, and increased in embryo by getting higher percentage of exotic germplasm. Contrary, phenolic content and DPPH radical scavenging activity of seeds, which were higher in adapted inbred lines than in DTP, were slightly decreased in their backcrosses with DTP.
AB  - U cilju proširenja genetičke osnove germplazme kukuruza (Zea mays L.), neophodna je inkorporacija egzotičnog u lokalni adaptirani selekcioni materijal. U radu su upoređeni antioksidativni sistemi dve lokalne inbred linije (A i B) sa antioksidativnim sistemima egzotične germplazme (DTP) i povratnih ukrštanja tih inbred linija i DTP-a (A1, A2, B1 i B2). U semenu kukuruza meren je sadržaj antioksidanata male molekulske težine, prolina i ukupnih fenola, kao i antioksidativni kapacitet, izražen preko sposobnosti hvatanja slobodnih DPPH radikala. Rezultati su pokazali porast nivoa prolina, kako u klici, tako i u endospermu, kod A1, A2, B1 i B2 genotipova, u odnosu na čiste inbred linije i DTP. Takođe, nivo prolina se povećavao sa povećanjem udela egzotične germplazme. Nasuprot tome, kod čistih inbred linija je bio veći nivo ukupnih fenola i stepen hvatanja slobodnih DPPH radikala nego kod DTP-a, i sa tendencijom neznatnog smanjenja kod A1, A2, B1 i B2 genotipova.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm
T1  - Sadržaj antioksidativnih komponenata u semenu genotipova kukuruza sa različitim udelom DTP-a
VL  - 41
IS  - 1
SP  - 21
EP  - 28
DO  - 10.2298/GENSR0901021K
ER  - 
@article{
author = "Kravić, Natalija and Anđelković, Violeta and Hadži-Tašković-Šukalović, Vesna and Vuletić, Mirjana",
year = "2009",
url = "http://rik.mrizp.rs/handle/123456789/290",
abstract = "In order to broaden the genetic base of maize (Zea mays L.) germplasm, it is necessary to integrate exotic materials into adapted breeding materials. The aim of the study was to compare antioxidative systems of two adapted maize inbred lines (A and B) with exotic germplasm, Drought Tolerant Population (DTP), and their backcrosses with DTP (A1, A2 and B1, B2). The content of low-molecular weight antioxidants, proline and phenolics, as well as antioxidant capacity, detected as free radical scavenging activities against DPPH radical, were measured in maize seeds. Proline content in both, embryo and endosperm was higher in backcrosses than in inbred lines and DTP, and increased in embryo by getting higher percentage of exotic germplasm. Contrary, phenolic content and DPPH radical scavenging activity of seeds, which were higher in adapted inbred lines than in DTP, were slightly decreased in their backcrosses with DTP., U cilju proširenja genetičke osnove germplazme kukuruza (Zea mays L.), neophodna je inkorporacija egzotičnog u lokalni adaptirani selekcioni materijal. U radu su upoređeni antioksidativni sistemi dve lokalne inbred linije (A i B) sa antioksidativnim sistemima egzotične germplazme (DTP) i povratnih ukrštanja tih inbred linija i DTP-a (A1, A2, B1 i B2). U semenu kukuruza meren je sadržaj antioksidanata male molekulske težine, prolina i ukupnih fenola, kao i antioksidativni kapacitet, izražen preko sposobnosti hvatanja slobodnih DPPH radikala. Rezultati su pokazali porast nivoa prolina, kako u klici, tako i u endospermu, kod A1, A2, B1 i B2 genotipova, u odnosu na čiste inbred linije i DTP. Takođe, nivo prolina se povećavao sa povećanjem udela egzotične germplazme. Nasuprot tome, kod čistih inbred linija je bio veći nivo ukupnih fenola i stepen hvatanja slobodnih DPPH radikala nego kod DTP-a, i sa tendencijom neznatnog smanjenja kod A1, A2, B1 i B2 genotipova.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm, Sadržaj antioksidativnih komponenata u semenu genotipova kukuruza sa različitim udelom DTP-a",
volume = "41",
number = "1",
pages = "21-28",
doi = "10.2298/GENSR0901021K"
}
Kravić, N., Anđelković, V., Hadži-Tašković-Šukalović, V.,& Vuletić, M. (2009). Sadržaj antioksidativnih komponenata u semenu genotipova kukuruza sa različitim udelom DTP-a.
Genetika
Društvo genetičara Srbije, Beograd., 41(1), 21-28.
https://doi.org/10.2298/GENSR0901021K
Kravić N, Anđelković V, Hadži-Tašković-Šukalović V, Vuletić M. Sadržaj antioksidativnih komponenata u semenu genotipova kukuruza sa različitim udelom DTP-a. Genetika. 2009;41(1):21-28
Kravić Natalija, Anđelković Violeta, Hadži-Tašković-Šukalović Vesna, Vuletić Mirjana, "Sadržaj antioksidativnih komponenata u semenu genotipova kukuruza sa različitim udelom DTP-a" Genetika, 41, no. 1 (2009):21-28,
https://doi.org/10.2298/GENSR0901021K .
2
5

Plasma-membrane-bound malate dehydrogenase activity in maize roots

Hadži-Tašković-Šukalović, Vesna; Vuletić, Mirjana; Ignjatović-Micić, Dragana; Vučinić, Željko

(1999)

TY  - JOUR
AU  - Hadži-Tašković-Šukalović, Vesna
AU  - Vuletić, Mirjana
AU  - Ignjatović-Micić, Dragana
AU  - Vučinić, Željko
PY  - 1999
UR  - http://rik.mrizp.rs/handle/123456789/6
AB  - Plasma membranes were isolated and purified from 14-day-old maize roots (Zea mays L.) by two-phase partitioning at a 6.5% polymer concentration, and compared to isolated mitochondria, microsomes, and soluble fraction. Marker enzyme analysis demonstrated that the plasma membranes were devoid of cytoplasmic, mitochondrial, tonoplast, and endoplasmic-reticulum contaminations. Isolated plasma membranes exhibited malate dehydrogenase activity, catalyzing NADH-dependent reduction of oxaloacetate as well as NAD + -dependent malate oxidation. Malate dehydrogenase activity was resistant to osmotic shock, freeze-thaw treatment, and salt washing and stimulated by solubilization with Triton X-100, indicating that the enzyme is tightly bound to the plasma membrane. Malate dehydrogenase activity was highly specific to NAD + and NADH. The enzyme exhibited a high degree of latency in both right-side-out (80%) and inside-out (70%) vesicle preparations. Kinetic and regulatory properties with ATP and P(i), as well as pH dependence of plasma-membrane-bound malate dehydrogenase were different from mitochondrial and soluble malate dehydrogenases. Starch gel electrophoresis revealed a characteristic isozyme form present in the plasma membrane isolate, but not present in the soluble, mitochondrial, and microsomal fractions. The results presented show that purified plasma membranes isolated from maize roots contain a tightly associated malate dehydrogenase, having properties different from mitochondrial and soluble malate dehydrogenases.
T2  - Protoplasma
T1  - Plasma-membrane-bound malate dehydrogenase activity in maize roots
VL  - 207
IS  - 3-4
SP  - 203
EP  - 212
ER  - 
@article{
author = "Hadži-Tašković-Šukalović, Vesna and Vuletić, Mirjana and Ignjatović-Micić, Dragana and Vučinić, Željko",
year = "1999",
url = "http://rik.mrizp.rs/handle/123456789/6",
abstract = "Plasma membranes were isolated and purified from 14-day-old maize roots (Zea mays L.) by two-phase partitioning at a 6.5% polymer concentration, and compared to isolated mitochondria, microsomes, and soluble fraction. Marker enzyme analysis demonstrated that the plasma membranes were devoid of cytoplasmic, mitochondrial, tonoplast, and endoplasmic-reticulum contaminations. Isolated plasma membranes exhibited malate dehydrogenase activity, catalyzing NADH-dependent reduction of oxaloacetate as well as NAD + -dependent malate oxidation. Malate dehydrogenase activity was resistant to osmotic shock, freeze-thaw treatment, and salt washing and stimulated by solubilization with Triton X-100, indicating that the enzyme is tightly bound to the plasma membrane. Malate dehydrogenase activity was highly specific to NAD + and NADH. The enzyme exhibited a high degree of latency in both right-side-out (80%) and inside-out (70%) vesicle preparations. Kinetic and regulatory properties with ATP and P(i), as well as pH dependence of plasma-membrane-bound malate dehydrogenase were different from mitochondrial and soluble malate dehydrogenases. Starch gel electrophoresis revealed a characteristic isozyme form present in the plasma membrane isolate, but not present in the soluble, mitochondrial, and microsomal fractions. The results presented show that purified plasma membranes isolated from maize roots contain a tightly associated malate dehydrogenase, having properties different from mitochondrial and soluble malate dehydrogenases.",
journal = "Protoplasma",
title = "Plasma-membrane-bound malate dehydrogenase activity in maize roots",
volume = "207",
number = "3-4",
pages = "203-212"
}
Hadži-Tašković-Šukalović, V., Vuletić, M., Ignjatović-Micić, D.,& Vučinić, Ž. (1999). Plasma-membrane-bound malate dehydrogenase activity in maize roots.
Protoplasma, 207(3-4), 203-212.
Hadži-Tašković-Šukalović V, Vuletić M, Ignjatović-Micić D, Vučinić Ž. Plasma-membrane-bound malate dehydrogenase activity in maize roots. Protoplasma. 1999;207(3-4):203-212
Hadži-Tašković-Šukalović Vesna, Vuletić Mirjana, Ignjatović-Micić Dragana, Vučinić Željko, "Plasma-membrane-bound malate dehydrogenase activity in maize roots" Protoplasma, 207, no. 3-4 (1999):203-212
13