Stanković, Goran

Link to this page

Authority KeyName Variants
orcid::0000-0002-7723-3238
  • Stanković, Goran (50)
Projects
Improvment of maize and soybean traits by molecular and conventional breeding Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods
Fizička hemija dinamičkih stanja i struktura neravnotežnih sistema - od monotone do oscilatorne evolucije i haosa Oplemenjivanje kukuruza specifičnih svojstava za industrijske potrebe
Razvoj tehnologije gajenja kukuruza sa ekološkim pristupom Identifikacija izvora tolerantnosti prema suši u gen banci kukuruza
Stvaranje linija i hibrida kukuruza visokog kvaliteta proteina Exploitation of maize diversity to improve grain quality and drought tolerance
Agricultural Experiment Station - 6699 Benefit-sharing Fund of the International Treaty on Plant Genetic Resources for Food and Agriculture
CREDIT Vibes—Twinning Green Editing Vibes for FuuD (grant no. 101059942) FAO Project
Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety Regulacija antioksidativnog metabolizma biljaka u toku rastenja, infekcije patogenima i delovanja abiotičkog stresa: mehanizmi transporta, signalizacije i otpornosti
Ispitivanja novih biosenzora za monitoring i dijagnostiku biljaka Proučavanje i čuvanje core kolekcije fitopatogenih gljiva značajnih u poljoprivredi Srbije
Maize Research Institute, Zemun Polje Ministry of Science and Technological Development of the Republic of Serbia - 03E211
Ministry of Science and Technological Development of the Republic of Serbia - 03E22 Ministry of Science, Technological Development and Innovation of the Republic of Serbia (grant no. 451-03-47/2023-01/200040)

Author's Bibliography

First report of Aspergillus welwitschiae causing maize ear rot in Serbia

Nikolić, Milica; Savić, Iva; Nikolić, Ana; Stevanović, Milan; Kandić, Vesna; Stanković, Goran; Stanković, Slavica

(The American Phytopathological Society, 2023)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Nikolić, Ana
AU  - Stevanović, Milan
AU  - Kandić, Vesna
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1315
AB  - In recent years, countries in Southeast Europe are facing climate changes characterized by extreme hot weather, which contribute to the increased frequency of Aspergillus species. Because of these changes, Aspergillus  parasiticus  was  isolated,  for  the  first  time,  from  maize  grain  in  Serbia (Nikolic et al, 2018). The presence of black powdery mycelia on maize ears indicated occurrence of species of the genus Aspergillus section Nigri, which led to the need for detailed identification of these fungi. Disease incidence ranged from 10 and 15% in August 2013. Maize ears with black powdery symptoms were collected from field in Zemun Polje, Serbia. Symptomatic kernels were surface  sterilized  with  1%  sodium  hypochlorite  solution  for  3  min,  rinsed  three  times  with sterilized  water,  then  incubated  at  25°C  in  the  dark  for  7  days  on  potato  dextrose  agar  (PDA). Twenty  isolates  were  identified  as  genus Aspergillus section Nigri.  Monospore  cultures  formed black cottony colonies with a yellowish border on PDA. The average colony diameter was 50 mm. In  order  to  reliably  identify,  isolates  were  transferred  to  Malt  Extract  agar  (MEA)  and  Czapek Yeast Autolysate agar (CYA) (Samson et al, 2014). On CYA fungal colonies consisted of a white mycelium, covered by a layer of black conidiophores. On MEA fungal colonies were dense, black, with  yellowish  border.  The  reverse  side  was  colorless  to  pale  yellow,  with  a  yellow  ring  in  the middle. The average size of conidia was 4.3 μm. The conidia were globose to sub-globose, smooth to  roughened,  which  coincides  with  previous  research  (Silva  et  al,  2020).  Given  that  the  fungi Aspergillus niger and Aspergillus welwitschiae are morphologically indistinguishable (Susca et al, 2016), species level identification was completed by analysis of a partial sequence of the internal Page 1 of 5transcribed spacer (ITS) region (ITS1/ITS4 primers) and calmodulin gene (CMD5/CMD6 primers) (Samson et al., 2014).  The sequences were compared with the sequences of A. welwitschiae strains registered in the GenBank database based on nucleotide similarity, and results showed 99,64 and 100% similarity with ITS (OL711714) and calmodulin (KX894585), respectively. The sequence was deposited in GenBank with accession numbers OQ456471 (ITS) and OQ426518 (calmodulin). We also confirmed the presence of this species with specific primers (AWEL1/AWEL2) designed by Susca et al. 2020. Pathogenicity test was performed in Zemun Polje on the same maize hybrid from which the fungal species was isolated. Using artificial inoculations by the injecting conidial suspension into the silk channel, three days after 50% of plants reached the silking stage. Twenty ears were inoculated with each isolate, in four replicates (Reid et al, 1996). Inoculum was prepared from 7-day-old colonies on PDA, and 2 ml of a conidial suspension (1×106 spores/ml) was used. Control plants were inoculated with sterile water. All inoculated ears showed symptoms, similar to those from field infections. Control ears were symptomless. The fungus was reisolated and was morphologically  identical  to  the  original  isolates,  thus  completing  Koch’s  postulates.  Based  on molecular,   morphological   and   pathogenic   properties,   the   isolates   were   identified   as A. welwitschiae. This is the first report of A. welwitschiae as the causal agent of black maize ear rot not only in Serbia, but also in the other countries of the Western Balkans. Given that the fungus A. welwitschiae  synthesizes  both  ochratoxin  A  (OTA)  (Battilani  et  al,  2006)  and  fumonisin  (FB) (Frisvad  et  al,  2011),  further  studies  should  be  focused  on  assessment  its  aggressiveness  and toxicological profile.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - First report of Aspergillus welwitschiae causing maize ear rot in Serbia
UR  - https://hdl.handle.net/21.15107/rcub_rik_1315
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Nikolić, Ana and Stevanović, Milan and Kandić, Vesna and Stanković, Goran and Stanković, Slavica",
year = "2023",
abstract = "In recent years, countries in Southeast Europe are facing climate changes characterized by extreme hot weather, which contribute to the increased frequency of Aspergillus species. Because of these changes, Aspergillus  parasiticus  was  isolated,  for  the  first  time,  from  maize  grain  in  Serbia (Nikolic et al, 2018). The presence of black powdery mycelia on maize ears indicated occurrence of species of the genus Aspergillus section Nigri, which led to the need for detailed identification of these fungi. Disease incidence ranged from 10 and 15% in August 2013. Maize ears with black powdery symptoms were collected from field in Zemun Polje, Serbia. Symptomatic kernels were surface  sterilized  with  1%  sodium  hypochlorite  solution  for  3  min,  rinsed  three  times  with sterilized  water,  then  incubated  at  25°C  in  the  dark  for  7  days  on  potato  dextrose  agar  (PDA). Twenty  isolates  were  identified  as  genus Aspergillus section Nigri.  Monospore  cultures  formed black cottony colonies with a yellowish border on PDA. The average colony diameter was 50 mm. In  order  to  reliably  identify,  isolates  were  transferred  to  Malt  Extract  agar  (MEA)  and  Czapek Yeast Autolysate agar (CYA) (Samson et al, 2014). On CYA fungal colonies consisted of a white mycelium, covered by a layer of black conidiophores. On MEA fungal colonies were dense, black, with  yellowish  border.  The  reverse  side  was  colorless  to  pale  yellow,  with  a  yellow  ring  in  the middle. The average size of conidia was 4.3 μm. The conidia were globose to sub-globose, smooth to  roughened,  which  coincides  with  previous  research  (Silva  et  al,  2020).  Given  that  the  fungi Aspergillus niger and Aspergillus welwitschiae are morphologically indistinguishable (Susca et al, 2016), species level identification was completed by analysis of a partial sequence of the internal Page 1 of 5transcribed spacer (ITS) region (ITS1/ITS4 primers) and calmodulin gene (CMD5/CMD6 primers) (Samson et al., 2014).  The sequences were compared with the sequences of A. welwitschiae strains registered in the GenBank database based on nucleotide similarity, and results showed 99,64 and 100% similarity with ITS (OL711714) and calmodulin (KX894585), respectively. The sequence was deposited in GenBank with accession numbers OQ456471 (ITS) and OQ426518 (calmodulin). We also confirmed the presence of this species with specific primers (AWEL1/AWEL2) designed by Susca et al. 2020. Pathogenicity test was performed in Zemun Polje on the same maize hybrid from which the fungal species was isolated. Using artificial inoculations by the injecting conidial suspension into the silk channel, three days after 50% of plants reached the silking stage. Twenty ears were inoculated with each isolate, in four replicates (Reid et al, 1996). Inoculum was prepared from 7-day-old colonies on PDA, and 2 ml of a conidial suspension (1×106 spores/ml) was used. Control plants were inoculated with sterile water. All inoculated ears showed symptoms, similar to those from field infections. Control ears were symptomless. The fungus was reisolated and was morphologically  identical  to  the  original  isolates,  thus  completing  Koch’s  postulates.  Based  on molecular,   morphological   and   pathogenic   properties,   the   isolates   were   identified   as A. welwitschiae. This is the first report of A. welwitschiae as the causal agent of black maize ear rot not only in Serbia, but also in the other countries of the Western Balkans. Given that the fungus A. welwitschiae  synthesizes  both  ochratoxin  A  (OTA)  (Battilani  et  al,  2006)  and  fumonisin  (FB) (Frisvad  et  al,  2011),  further  studies  should  be  focused  on  assessment  its  aggressiveness  and toxicological profile.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Aspergillus welwitschiae causing maize ear rot in Serbia",
url = "https://hdl.handle.net/21.15107/rcub_rik_1315"
}
Nikolić, M., Savić, I., Nikolić, A., Stevanović, M., Kandić, V., Stanković, G.,& Stanković, S.. (2023). First report of Aspergillus welwitschiae causing maize ear rot in Serbia. in Plant Disease
The American Phytopathological Society..
https://hdl.handle.net/21.15107/rcub_rik_1315
Nikolić M, Savić I, Nikolić A, Stevanović M, Kandić V, Stanković G, Stanković S. First report of Aspergillus welwitschiae causing maize ear rot in Serbia. in Plant Disease. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1315 .
Nikolić, Milica, Savić, Iva, Nikolić, Ana, Stevanović, Milan, Kandić, Vesna, Stanković, Goran, Stanković, Slavica, "First report of Aspergillus welwitschiae causing maize ear rot in Serbia" in Plant Disease (2023),
https://hdl.handle.net/21.15107/rcub_rik_1315 .

ZP 5640

Stanković, Goran; Stanković, Slavica

(Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja, 2023)

TY  - PAT
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2023
UR  - http://rik.mrizp.rs/handle/123456789/1270
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-09-02464/2/2021-11 od 30.03.2023. godine
T1  - ZP 5640
UR  - https://hdl.handle.net/21.15107/rcub_rik_1270
ER  - 
@misc{
author = "Stanković, Goran and Stanković, Slavica",
year = "2023",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-09-02464/2/2021-11 od 30.03.2023. godine",
title = "ZP 5640",
url = "https://hdl.handle.net/21.15107/rcub_rik_1270"
}
Stanković, G.,& Stanković, S.. (2023). ZP 5640. in Rešenje broj 320-09-02464/2/2021-11 od 30.03.2023. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1270
Stanković G, Stanković S. ZP 5640. in Rešenje broj 320-09-02464/2/2021-11 od 30.03.2023. godine. 2023;.
https://hdl.handle.net/21.15107/rcub_rik_1270 .
Stanković, Goran, Stanković, Slavica, "ZP 5640" in Rešenje broj 320-09-02464/2/2021-11 od 30.03.2023. godine (2023),
https://hdl.handle.net/21.15107/rcub_rik_1270 .

ZP569

Stanković, Goran; Nikolić, Milica

(2022)

TY  - PAT
AU  - Stanković, Goran
AU  - Nikolić, Milica
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1096
T2  - Rešenje:320-44-02156/2/2020-11
T1  - ZP569
UR  - https://hdl.handle.net/21.15107/rcub_rik_1096
ER  - 
@misc{
author = "Stanković, Goran and Nikolić, Milica",
year = "2022",
journal = "Rešenje:320-44-02156/2/2020-11",
title = "ZP569",
url = "https://hdl.handle.net/21.15107/rcub_rik_1096"
}
Stanković, G.,& Nikolić, M.. (2022). ZP569. in Rešenje:320-44-02156/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1096
Stanković G, Nikolić M. ZP569. in Rešenje:320-44-02156/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1096 .
Stanković, Goran, Nikolić, Milica, "ZP569" in Rešenje:320-44-02156/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1096 .

ZP565

Stanković, Goran; Stanković, Slavica

(2022)

TY  - PAT
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1095
T2  - Rešenje:320-44-02152/2/2020-11
T1  - ZP565
UR  - https://hdl.handle.net/21.15107/rcub_rik_1095
ER  - 
@misc{
author = "Stanković, Goran and Stanković, Slavica",
year = "2022",
journal = "Rešenje:320-44-02152/2/2020-11",
title = "ZP565",
url = "https://hdl.handle.net/21.15107/rcub_rik_1095"
}
Stanković, G.,& Stanković, S.. (2022). ZP565. in Rešenje:320-44-02152/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1095
Stanković G, Stanković S. ZP565. in Rešenje:320-44-02152/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1095 .
Stanković, Goran, Stanković, Slavica, "ZP565" in Rešenje:320-44-02152/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1095 .

ZP5252

Stevanović, Milan; Delić, Nenad; Filipović, Milomir; Stanković, Goran

(2022)

TY  - PAT
AU  - Stevanović, Milan
AU  - Delić, Nenad
AU  - Filipović, Milomir
AU  - Stanković, Goran
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/1104
T2  - Rešenje:320-44-02165/2/2020-11
T1  - ZP5252
UR  - https://hdl.handle.net/21.15107/rcub_rik_1104
ER  - 
@misc{
author = "Stevanović, Milan and Delić, Nenad and Filipović, Milomir and Stanković, Goran",
year = "2022",
journal = "Rešenje:320-44-02165/2/2020-11",
title = "ZP5252",
url = "https://hdl.handle.net/21.15107/rcub_rik_1104"
}
Stevanović, M., Delić, N., Filipović, M.,& Stanković, G.. (2022). ZP5252. in Rešenje:320-44-02165/2/2020-11.
https://hdl.handle.net/21.15107/rcub_rik_1104
Stevanović M, Delić N, Filipović M, Stanković G. ZP5252. in Rešenje:320-44-02165/2/2020-11. 2022;.
https://hdl.handle.net/21.15107/rcub_rik_1104 .
Stevanović, Milan, Delić, Nenad, Filipović, Milomir, Stanković, Goran, "ZP5252" in Rešenje:320-44-02165/2/2020-11 (2022),
https://hdl.handle.net/21.15107/rcub_rik_1104 .

First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia

Obradović, Ana; Stepanović, Jelena; Krnjaja, Vesna; Bulajić, Aleksandra; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : APS publications, 2022)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stepanović, Jelena
AU  - Krnjaja, Vesna
AU  - Bulajić, Aleksandra
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/853
PB  - St. Paul : APS publications
T2  - Plant Disease
T1  - First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia
VL  - 106
IS  - 2
SP  - 758
EP  - 758
DO  - 10.1094/PDIS-04-21-0715-PDN
ER  - 
@article{
author = "Obradović, Ana and Stepanović, Jelena and Krnjaja, Vesna and Bulajić, Aleksandra and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2022",
publisher = "St. Paul : APS publications",
journal = "Plant Disease",
title = "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia",
volume = "106",
number = "2",
pages = "758-758",
doi = "10.1094/PDIS-04-21-0715-PDN"
}
Obradović, A., Stepanović, J., Krnjaja, V., Bulajić, A., Stanković, G., Stevanović, M.,& Stanković, S.. (2022). First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease
St. Paul : APS publications., 106(2), 758-758.
https://doi.org/10.1094/PDIS-04-21-0715-PDN
Obradović A, Stepanović J, Krnjaja V, Bulajić A, Stanković G, Stevanović M, Stanković S. First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia. in Plant Disease. 2022;106(2):758-758.
doi:10.1094/PDIS-04-21-0715-PDN .
Obradović, Ana, Stepanović, Jelena, Krnjaja, Vesna, Bulajić, Aleksandra, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First Report of Head Blight of Wheat Caused by Fusarium vorosii in Serbia" in Plant Disease, 106, no. 2 (2022):758-758,
https://doi.org/10.1094/PDIS-04-21-0715-PDN . .
2

ZP795

Stanković, Goran; Delić, Nenad

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1090
T2  - Rešenje:320-04-02287/2/2019-11
T1  - ZP795
UR  - https://hdl.handle.net/21.15107/rcub_rik_1090
ER  - 
@misc{
author = "Stanković, Goran and Delić, Nenad",
year = "2021",
journal = "Rešenje:320-04-02287/2/2019-11",
title = "ZP795",
url = "https://hdl.handle.net/21.15107/rcub_rik_1090"
}
Stanković, G.,& Delić, N.. (2021). ZP795. in Rešenje:320-04-02287/2/2019-11.
https://hdl.handle.net/21.15107/rcub_rik_1090
Stanković G, Delić N. ZP795. in Rešenje:320-04-02287/2/2019-11. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1090 .
Stanković, Goran, Delić, Nenad, "ZP795" in Rešenje:320-04-02287/2/2019-11 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1090 .

ZP711

Stanković, Goran

(2021)

TY  - PAT
AU  - Stanković, Goran
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1088
T2  - Rešenje:320-04-02289/2/2019-11
T1  - ZP711
UR  - https://hdl.handle.net/21.15107/rcub_rik_1088
ER  - 
@misc{
author = "Stanković, Goran",
year = "2021",
journal = "Rešenje:320-04-02289/2/2019-11",
title = "ZP711",
url = "https://hdl.handle.net/21.15107/rcub_rik_1088"
}
Stanković, G.. (2021). ZP711. in Rešenje:320-04-02289/2/2019-11.
https://hdl.handle.net/21.15107/rcub_rik_1088
Stanković G. ZP711. in Rešenje:320-04-02289/2/2019-11. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1088 .
Stanković, Goran, "ZP711" in Rešenje:320-04-02289/2/2019-11 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1088 .

ZP710

Stanković, Goran; Filipović, Milomir; Obradović, Ana

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Filipović, Milomir
AU  - Obradović, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1087
T2  - Rešenje:320-04-02279/2/2019-11
T1  - ZP710
UR  - https://hdl.handle.net/21.15107/rcub_rik_1087
ER  - 
@misc{
author = "Stanković, Goran and Filipović, Milomir and Obradović, Ana",
year = "2021",
journal = "Rešenje:320-04-02279/2/2019-11",
title = "ZP710",
url = "https://hdl.handle.net/21.15107/rcub_rik_1087"
}
Stanković, G., Filipović, M.,& Obradović, A.. (2021). ZP710. in Rešenje:320-04-02279/2/2019-11.
https://hdl.handle.net/21.15107/rcub_rik_1087
Stanković G, Filipović M, Obradović A. ZP710. in Rešenje:320-04-02279/2/2019-11. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1087 .
Stanković, Goran, Filipović, Milomir, Obradović, Ana, "ZP710" in Rešenje:320-04-02279/2/2019-11 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1087 .

ZP568

Stanković, Goran; Filipović, Milomir; Obradović, Ana

(2021)

TY  - PAT
AU  - Stanković, Goran
AU  - Filipović, Milomir
AU  - Obradović, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/1078
T2  - Rešenje:320-04-02281/2/2019-11
T1  - ZP568
UR  - https://hdl.handle.net/21.15107/rcub_rik_1078
ER  - 
@misc{
author = "Stanković, Goran and Filipović, Milomir and Obradović, Ana",
year = "2021",
journal = "Rešenje:320-04-02281/2/2019-11",
title = "ZP568",
url = "https://hdl.handle.net/21.15107/rcub_rik_1078"
}
Stanković, G., Filipović, M.,& Obradović, A.. (2021). ZP568. in Rešenje:320-04-02281/2/2019-11.
https://hdl.handle.net/21.15107/rcub_rik_1078
Stanković G, Filipović M, Obradović A. ZP568. in Rešenje:320-04-02281/2/2019-11. 2021;.
https://hdl.handle.net/21.15107/rcub_rik_1078 .
Stanković, Goran, Filipović, Milomir, Obradović, Ana, "ZP568" in Rešenje:320-04-02281/2/2019-11 (2021),
https://hdl.handle.net/21.15107/rcub_rik_1078 .

Agronomic traits of QPM maize hybrids adapted to temperate regions

Vančetović, Jelena; Ignjatović-Micić, Dragana; Kostadinović, Marija; Delić, Nenad; Stanković, Goran; Božinović, Sofija; Đorđević-Melnik, Olivera

(Zagreb : University of Zagreb, Faculty of Agriculture, 2020)

TY  - CONF
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Delić, Nenad
AU  - Stanković, Goran
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/1124
AB  - In this paper agronomic characteristics of quality protein maize (QPM) hybrids adapted to temperate
regions created in Maize Research Institut Zemun Polje are presented. Hybrid ZPQPM13 showed
favorable traits for growing in these regions, and its grain yield was at the level of one of the leading
ZP hybrids - ZP 606. However, grain moisture of this hybrid was 3% higher than that of ZP 606,
pointing out to the tropical origin of its parental inbred lines. Namely, mother was obtained from F1
cross between tropical and adapted lines, and father by marker assisted selection (MAS) from QPM
line of tropical origin that was crossed and twice backcrossed with a commercial ZP line. In the future,
in order to obtain even more adapted QPM hybrids, MAS program will continue with all the best
commercial ZP inbred lines of all FAO groups.
PB  - Zagreb : University of Zagreb, Faculty of Agriculture
C3  - Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia
T1  - Agronomic traits of QPM maize hybrids adapted to temperate regions
SP  - 289
EP  - 293
UR  - https://hdl.handle.net/21.15107/rcub_rik_1124
ER  - 
@conference{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Kostadinović, Marija and Delić, Nenad and Stanković, Goran and Božinović, Sofija and Đorđević-Melnik, Olivera",
year = "2020",
abstract = "In this paper agronomic characteristics of quality protein maize (QPM) hybrids adapted to temperate
regions created in Maize Research Institut Zemun Polje are presented. Hybrid ZPQPM13 showed
favorable traits for growing in these regions, and its grain yield was at the level of one of the leading
ZP hybrids - ZP 606. However, grain moisture of this hybrid was 3% higher than that of ZP 606,
pointing out to the tropical origin of its parental inbred lines. Namely, mother was obtained from F1
cross between tropical and adapted lines, and father by marker assisted selection (MAS) from QPM
line of tropical origin that was crossed and twice backcrossed with a commercial ZP line. In the future,
in order to obtain even more adapted QPM hybrids, MAS program will continue with all the best
commercial ZP inbred lines of all FAO groups.",
publisher = "Zagreb : University of Zagreb, Faculty of Agriculture",
journal = "Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia",
title = "Agronomic traits of QPM maize hybrids adapted to temperate regions",
pages = "289-293",
url = "https://hdl.handle.net/21.15107/rcub_rik_1124"
}
Vančetović, J., Ignjatović-Micić, D., Kostadinović, M., Delić, N., Stanković, G., Božinović, S.,& Đorđević-Melnik, O.. (2020). Agronomic traits of QPM maize hybrids adapted to temperate regions. in Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia
Zagreb : University of Zagreb, Faculty of Agriculture., 289-293.
https://hdl.handle.net/21.15107/rcub_rik_1124
Vančetović J, Ignjatović-Micić D, Kostadinović M, Delić N, Stanković G, Božinović S, Đorđević-Melnik O. Agronomic traits of QPM maize hybrids adapted to temperate regions. in Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia. 2020;:289-293.
https://hdl.handle.net/21.15107/rcub_rik_1124 .
Vančetović, Jelena, Ignjatović-Micić, Dragana, Kostadinović, Marija, Delić, Nenad, Stanković, Goran, Božinović, Sofija, Đorđević-Melnik, Olivera, "Agronomic traits of QPM maize hybrids adapted to temperate regions" in Proceedings of 55th Croatian and 15th International Symposium on Agriculture, February 16-21, 2020., Vodice, Croatia (2020):289-293,
https://hdl.handle.net/21.15107/rcub_rik_1124 .

First report of Aspergillus parasiticus on Barley Grain in Serbia

Nikolić, Milica; Savić, Iva; Obradović, Ana; Srdić, Jelena; Stanković, Goran; Stevanović, Milan; Stanković, Slavica

(St. Paul : The American Phytopathological Society (APS), 2020)

TY  - JOUR
AU  - Nikolić, Milica
AU  - Savić, Iva
AU  - Obradović, Ana
AU  - Srdić, Jelena
AU  - Stanković, Goran
AU  - Stevanović, Milan
AU  - Stanković, Slavica
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/796
AB  - Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.
PB  - St. Paul : The American Phytopathological Society (APS)
T2  - Plant Disease
T1  - First report of Aspergillus parasiticus on Barley Grain in Serbia
VL  - 104
IS  - 3
SP  - 987
DO  - 10.1094/PDIS-07-19-1364-PDN
ER  - 
@article{
author = "Nikolić, Milica and Savić, Iva and Obradović, Ana and Srdić, Jelena and Stanković, Goran and Stevanović, Milan and Stanković, Slavica",
year = "2020",
abstract = "Barley (Hordeum vulgare L.) is a secondary grain crop in Serbia used commercially for animal feed, seed, and human food applications. The production of barley in the 2016 to 2017 growing season reached a record yield of almost 400,000 metric tons (USDA 2017). Aspergillus contamination has been rare in the agroecological conditions of cereal-growing areas in Serbia. Changes in climatic factors, such as occurrence of high temperatures and prolonged droughts, increased frequency of Aspergillus spp. Species Aspergillus parasiticus was isolated from maize grain for the first time in Serbia in 2012 and from wheat grains in 2017 (Nikolic et al. 2018). We hypothesized that these pathogens can also be present in barley fields in Serbia. Barley spikes exhibiting bleaching were sampled at the beginning of June 2017 grown in northern Serbia. In severe infections, barley spikes get a dry look with awns that stand upright and firm. The incidence of the disease of the bleached spiked in the field was 15 to 20%. From each representative sample, 100 shriveled grains were collected. After surface sterilization with bleach/distilled water 1:3, 100 grains per sample (10 per Petri dish) were placed on potato dextrose agar and incubated at 25°C for 7 days. After isolation, 50% of isolates were identified as Alternaria spp., 20% as Fusarium spp., approximately 15% as Aspergillus spp. In order to reliably identify individual species of fungi, the fragments of colonies were transferred to malt extract agar (MEA) and Czapek yeast agar (CYA) and incubated in the dark for 7 days. The fungal colonies were dark green. The reverse side was pale yellow. The average colony diameter was 65 mm. Conidia were spherical and rough with thick walls. The average size of conidia was 5.1 µm. Colonies were floccose and thin on MEA and CYA. Based on growth and morphological characteristics, isolates were determined as A. parasiticus (Pitt and Hocking 2009). Molecular detection of Aspergillus species was done by using PCR-restriction fragment length polymorphism analysis of the aflR-aflJ (genes for aflatoxin biosynthesis) intergenic spacer. The restriction enzyme BglII was able to cut the PCR product of A. parasiticus at one restriction site, resulting in two fragments of 363 and 311 bp (El Khoury et al. 2011). A. parasiticus CBS 100926 was used as a reference isolate. The pathogenicity of 20 isolates was verified on a group of 20 randomly selected spikes in four replicates (Mesterházy et al. 1999). A 7-day-old culture of each isolate was used for the preparation of the spore suspension (1 × 10⁶ spores/ml). Inoculation was carried out after 50% of plants reached the anthesis stage. Groups of 20 selected spikes were sprayed from all sides with 20 ml of fungal spore suspension. Control spikes were inoculated by applying an equal amount of sterile distilled water. The infection rate was estimated after 3 weeks on a 1 to 7 scale, with 1 = 0 to 5%, 2 = 5 to 15%, 3 = 15 to 30%, 4 = 30 to 50%, 5 = 50 to 75%, 6 = 75 to 90%, and 7 = 90 to 100% infected spike area. The average infection rate was 3.2. The pathogen was reisolated from the inoculated spikes and identified as A. parasiticus, with the aim to confirm Koch’s postulates. Developed symptoms were similar to those observed on spikes collected from the field. Control spikes did not show any symptoms of the disease. These results confirmed the pathogenicity of A. parasiticus on H. vulgare. To the best of our knowledge, this is the first report of the occurrence of A. parasiticus on barley grain in Serbia. Because A. parasiticus is known to be a severe aflatoxin producer and climatic changes can increase the frequency of this fungus, further studies are necessary to improve strategies for food safety and quality.",
publisher = "St. Paul : The American Phytopathological Society (APS)",
journal = "Plant Disease",
title = "First report of Aspergillus parasiticus on Barley Grain in Serbia",
volume = "104",
number = "3",
pages = "987",
doi = "10.1094/PDIS-07-19-1364-PDN"
}
Nikolić, M., Savić, I., Obradović, A., Srdić, J., Stanković, G., Stevanović, M.,& Stanković, S.. (2020). First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease
St. Paul : The American Phytopathological Society (APS)., 104(3), 987.
https://doi.org/10.1094/PDIS-07-19-1364-PDN
Nikolić M, Savić I, Obradović A, Srdić J, Stanković G, Stevanović M, Stanković S. First report of Aspergillus parasiticus on Barley Grain in Serbia. in Plant Disease. 2020;104(3):987.
doi:10.1094/PDIS-07-19-1364-PDN .
Nikolić, Milica, Savić, Iva, Obradović, Ana, Srdić, Jelena, Stanković, Goran, Stevanović, Milan, Stanković, Slavica, "First report of Aspergillus parasiticus on Barley Grain in Serbia" in Plant Disease, 104, no. 3 (2020):987,
https://doi.org/10.1094/PDIS-07-19-1364-PDN . .
3
1

ZP 709

Stanković, Goran

(Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja, 2019)

TY  - PAT
AU  - Stanković, Goran
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1210
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-04-02409/2/2016-11 od 11.03.2019. godine
T1  - ZP 709
UR  - https://hdl.handle.net/21.15107/rcub_rik_1210
ER  - 
@misc{
author = "Stanković, Goran",
year = "2019",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-04-02409/2/2016-11 od 11.03.2019. godine",
title = "ZP 709",
url = "https://hdl.handle.net/21.15107/rcub_rik_1210"
}
Stanković, G.. (2019). ZP 709. in Rešenje broj 320-04-02409/2/2016-11 od 11.03.2019. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1210
Stanković G. ZP 709. in Rešenje broj 320-04-02409/2/2016-11 od 11.03.2019. godine. 2019;.
https://hdl.handle.net/21.15107/rcub_rik_1210 .
Stanković, Goran, "ZP 709" in Rešenje broj 320-04-02409/2/2016-11 od 11.03.2019. godine (2019),
https://hdl.handle.net/21.15107/rcub_rik_1210 .

ZP 7100

Stanković, Goran; Stevanović, Milan

(Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja, 2019)

TY  - PAT
AU  - Stanković, Goran
AU  - Stevanović, Milan
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1228
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-04-02195/2/2017-11 od 20.02.2019. godine
T1  - ZP 7100
UR  - https://hdl.handle.net/21.15107/rcub_rik_1228
ER  - 
@misc{
author = "Stanković, Goran and Stevanović, Milan",
year = "2019",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-04-02195/2/2017-11 od 20.02.2019. godine",
title = "ZP 7100",
url = "https://hdl.handle.net/21.15107/rcub_rik_1228"
}
Stanković, G.,& Stevanović, M.. (2019). ZP 7100. in Rešenje broj 320-04-02195/2/2017-11 od 20.02.2019. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1228
Stanković G, Stevanović M. ZP 7100. in Rešenje broj 320-04-02195/2/2017-11 od 20.02.2019. godine. 2019;.
https://hdl.handle.net/21.15107/rcub_rik_1228 .
Stanković, Goran, Stevanović, Milan, "ZP 7100" in Rešenje broj 320-04-02195/2/2017-11 od 20.02.2019. godine (2019),
https://hdl.handle.net/21.15107/rcub_rik_1228 .

ZP 794

Stanković, Goran

(Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja, 2019)

TY  - PAT
AU  - Stanković, Goran
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/1212
PB  - Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja
T2  - Rešenje broj 320-04-02408/2/2016-11 od 11.03.2019. godine
T1  - ZP 794
UR  - https://hdl.handle.net/21.15107/rcub_rik_1212
ER  - 
@misc{
author = "Stanković, Goran",
year = "2019",
publisher = "Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja",
journal = "Rešenje broj 320-04-02408/2/2016-11 od 11.03.2019. godine",
title = "ZP 794",
url = "https://hdl.handle.net/21.15107/rcub_rik_1212"
}
Stanković, G.. (2019). ZP 794. in Rešenje broj 320-04-02408/2/2016-11 od 11.03.2019. godine
Beograd : Republika Srbija, Ministarstvo poljoprivrede, šumarstva i vodoprivrede - Uprava za zaštitu bilja..
https://hdl.handle.net/21.15107/rcub_rik_1212
Stanković G. ZP 794. in Rešenje broj 320-04-02408/2/2016-11 od 11.03.2019. godine. 2019;.
https://hdl.handle.net/21.15107/rcub_rik_1212 .
Stanković, Goran, "ZP 794" in Rešenje broj 320-04-02408/2/2016-11 od 11.03.2019. godine (2019),
https://hdl.handle.net/21.15107/rcub_rik_1212 .

Estimation of genetic diversity among maize inbred lines

Ristić, Danijela; Ignjatovic-Micic, Dragana; Mladenović Drinić, Snežana; Obradović, Ana; Kostadinović, Marija; Stevanović, Milan; Stanković, Goran

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2018)

TY  - CONF
AU  - Ristić, Danijela
AU  - Ignjatovic-Micic, Dragana
AU  - Mladenović Drinić, Snežana
AU  - Obradović, Ana
AU  - Kostadinović, Marija
AU  - Stevanović, Milan
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/976
AB  - One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
T1  - Estimation of genetic diversity among maize inbred lines
SP  - 280
EP  - 284
UR  - https://hdl.handle.net/21.15107/rcub_rik_976
ER  - 
@conference{
author = "Ristić, Danijela and Ignjatovic-Micic, Dragana and Mladenović Drinić, Snežana and Obradović, Ana and Kostadinović, Marija and Stevanović, Milan and Stanković, Goran",
year = "2018",
abstract = "One of the most effective method in maize (Zea mays L) selection of adapted material is to
create synthetic populations from inbred lines of known origin and superior properties. The
methods used for inter- and intra-population synthetic improvement are some of the recurrent
selection techniques. Success of recurrent selection depends on the choice of parent
components and the method that will be used to obtain new inbred lines. The aim of this paper
was to apply molecular markers for estimation of genetic variability of inbred lines, as
potential parent components of synthetic populations. Molecular characterization of 26 inbred
lines was done with 18 polymorphic SSR (Simple Sequence Repeat) markers. The total
number of obtained alleles was 54, and ranged from two alleles for primers: phi033, phi036,
phi087 and umc1013 to five alleles for primer umc1040. Genetic similarity values were
calculated using Dice coefficient in the NTSYSpc2 program package. The highest similarity
value (0.96) was calculated between inbred lines L22 and L24, while the lowest value (0.26)
was between inbred lines L7 and L21. Cluster analysis divided the inbred lines into three
groups mostly in accordance with their origin. The variability detected using SSR markers
could be useful in selecting best parental combinations in creating synthetic populations.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina",
title = "Estimation of genetic diversity among maize inbred lines",
pages = "280-284",
url = "https://hdl.handle.net/21.15107/rcub_rik_976"
}
Ristić, D., Ignjatovic-Micic, D., Mladenović Drinić, S., Obradović, A., Kostadinović, M., Stevanović, M.,& Stanković, G.. (2018). Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 280-284.
https://hdl.handle.net/21.15107/rcub_rik_976
Ristić D, Ignjatovic-Micic D, Mladenović Drinić S, Obradović A, Kostadinović M, Stevanović M, Stanković G. Estimation of genetic diversity among maize inbred lines. in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina. 2018;:280-284.
https://hdl.handle.net/21.15107/rcub_rik_976 .
Ristić, Danijela, Ignjatovic-Micic, Dragana, Mladenović Drinić, Snežana, Obradović, Ana, Kostadinović, Marija, Stevanović, Milan, Stanković, Goran, "Estimation of genetic diversity among maize inbred lines" in 9. International agricultural symposium “Agrosym 2018”, 04-07.2018, Jahorina, Bosnia and Herzegovina (2018):280-284,
https://hdl.handle.net/21.15107/rcub_rik_976 .

Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence

Obradović, Ana; Krnjaja, Vesna; Nikolić, Milica; Delibašić, Goran; Filipović, Milomir; Stanković, Goran; Stanković, Slavica

(Institut za stočarstvo, Beograd, 2018)

TY  - JOUR
AU  - Obradović, Ana
AU  - Krnjaja, Vesna
AU  - Nikolić, Milica
AU  - Delibašić, Goran
AU  - Filipović, Milomir
AU  - Stanković, Goran
AU  - Stanković, Slavica
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/738
AB  - Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species.
AB  - Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence
T1  - Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava
VL  - 34
IS  - 4
SP  - 469
EP  - 480
DO  - 10.2298/BAH1804469O
ER  - 
@article{
author = "Obradović, Ana and Krnjaja, Vesna and Nikolić, Milica and Delibašić, Goran and Filipović, Milomir and Stanković, Goran and Stanković, Slavica",
year = "2018",
abstract = "Agroecological and climatic conditions in Serbia greatly affected the development of toxigenic fungi and occurrence of mycotoxins in the maize. The presence of fungal toxigenic Aspergillus and Fusarium species and levels of aflatoxin B 1 (AFB 1) and sum of fumonisins B 1 , B 2 and B 3 (FBs) were established in 127 maize kernel samples harvested during 2012 (37 samples) and 2013 (90 samples). The periods of silking and grain filling of the maize in 2012 in comparison to 2013 were characterised with extremely dry spells, with high temperatures and low precipitation sums. The mean incidences of A. flavus and F. verticillioides were 50.4 and 11.7% in 2012 and 18.9 and 33.4% in 2013, respectively. According to the regulations of the World Health Organisation, unacceptable levels of AFB 1 (>20 μg kg-1) and FBs (>2000 μg kg-1) were established in the 30.6 and 24.1% samples of 2012 and 16.7 and 40% maize kernel samples of 2013, respectively. It can be concluded that high temperatures and low precipitation sums in 2012 favoured the development of A. flavus affecting the high level of AFB 1 , in comparison with F. verticillioides and the production of FBs. There was no positive correlation between the incidences of A. flavus and F. verticillioides, while a statistically significant positive correlation has been found between AFB 1 and FBs levels, in both investigated years (2012-2013). This indicates that the mycotoxin production depended more on weather conditions than on the distribution of corresponding toxigenic fungal species., Agroekološki i klimatski uslovi u Srbiji veoma su pogodni za razvoj toksigenih gljiva i njihovih mikotoksina u kukuruzu. Prisustvo toksigenih vrsta gljiva iz rodova Aspergillus i Fusarium, kao i nivoi aflatoksina B1 (AFB1) i ukupnih fumonizina B1, B2 i B3 (FBs) utvrđeni su u 127 uzoraka zrna kukuruza iz berbe tokom 2012 (37 uzoraka) i 2013. godine (90 uzoraka). U fenofazama svilanja i nalivanja zrna kukuruza u 2012. u odnosu na 2013. godinu zapaženi su ekstremno sušni periodi sa visokim temperaturama i niskim količinama padavina. Prosečne vrednosti za učestalost vrsta A. flavus i F. verticillioides bile su 50,4 i 11,7% u 2012., odnosno 18,9 i 33,4% u 2013. godini. Prema pravilniku Svetske zdravstvene organizacije (WHO) nedozvoljeni nivoi AFB1 (>20 µg/kg) i FBs (>2000 µg/kg) utvrđeni su u 30,6% i 24,1% uzoraka u 2012., odnosno u 16,7% i 40% uzoraka u 2013. godini. Na osnovu dobijenih rezultata zaključeno je da su visoke temperature i niske količine padavina u 2012. godini uslovile značajno veću učestalost A. flavus i visoku produkciju AFB1 u poredjenju sa vrstom F. verticillioides i produkcijom FBs. U obe ispitivane godine (2012-2013), između učestalosti A. flavus i F. verticillioides nije ustanovljena pozitivna korelacija, dok je statistički značajna pozitivna korelacija ustanovljena između nivoa AFB1 i FBs. Ovo ukazuje da produkcija mikotoksina je više zavisna od vremenskih uslova u odnosu na distribuciju toksigenih vrsta gljiva.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence, Uticaj klimatskih uslova na kontaminaciju zrna kukuruza sa aflatoksinom B1 i fumonizinima i njihova združena pojava",
volume = "34",
number = "4",
pages = "469-480",
doi = "10.2298/BAH1804469O"
}
Obradović, A., Krnjaja, V., Nikolić, M., Delibašić, G., Filipović, M., Stanković, G.,& Stanković, S.. (2018). Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 34(4), 469-480.
https://doi.org/10.2298/BAH1804469O
Obradović A, Krnjaja V, Nikolić M, Delibašić G, Filipović M, Stanković G, Stanković S. Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence. in Biotechnology in Animal Husbandry. 2018;34(4):469-480.
doi:10.2298/BAH1804469O .
Obradović, Ana, Krnjaja, Vesna, Nikolić, Milica, Delibašić, Goran, Filipović, Milomir, Stanković, Goran, Stanković, Slavica, "Impacts of climatic conditions on aflatoxin B 1 and fumonisins contamination of maize kernels and their co-occurrence" in Biotechnology in Animal Husbandry, 34, no. 4 (2018):469-480,
https://doi.org/10.2298/BAH1804469O . .
12

Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Stanković, Goran; Delić, Nenad

(Beograd : Društvo genetičara Srbije, 2018)

TY  - GEN
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Delić, Nenad
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/961
AB  - Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića.
AB  - Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
T2  - 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
T1  - Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna
T1  - Marker assisted selection for improving protein quality of maize grain
SP  - 170
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_rik_961
ER  - 
@misc{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Stanković, Goran and Delić, Nenad",
year = "2018",
abstract = "Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića., Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata",
title = "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna, Marker assisted selection for improving protein quality of maize grain",
pages = "170-171",
url = "https://hdl.handle.net/21.15107/rcub_rik_961"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Stanković, G.,& Delić, N.. (2018). Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
Beograd : Društvo genetičara Srbije., 170-171.
https://hdl.handle.net/21.15107/rcub_rik_961
Kostadinović M, Ignjatović-Micić D, Vančetović J, Stanković G, Delić N. Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata. 2018;:170-171.
https://hdl.handle.net/21.15107/rcub_rik_961 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Stanković, Goran, Delić, Nenad, "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna" in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata (2018):170-171,
https://hdl.handle.net/21.15107/rcub_rik_961 .

Assessment stability of maize lines yield by GGE-biplot analysis

Božović, Dragan; Zivanović, Tomislav; Popović, Vera; Tatić, Mladen; Gospavić, Zagorka; Miloradović, Zoran; Dokić, Milorad; Stanković, Goran

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Božović, Dragan
AU  - Zivanović, Tomislav
AU  - Popović, Vera
AU  - Tatić, Mladen
AU  - Gospavić, Zagorka
AU  - Miloradović, Zoran
AU  - Dokić, Milorad
AU  - Stanković, Goran
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/706
AB  - Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment stability of maize lines yield by GGE-biplot analysis
VL  - 50
IS  - 3
SP  - 755
EP  - 770
DO  - 10.2298/GENSR1803755B
ER  - 
@article{
author = "Božović, Dragan and Zivanović, Tomislav and Popović, Vera and Tatić, Mladen and Gospavić, Zagorka and Miloradović, Zoran and Dokić, Milorad and Stanković, Goran",
year = "2018",
abstract = "Maize genotypes have varied reactions in different localities, years, treatments or the combination of these factors, due to genotype x year interaction. The objective of this study was to estimate genotype by locality, by year, by treatments (GxLxYxT) interaction using AMMI model, to identify maize genotypes with stable and high yield performance in different growing seasons. The trials with seven maize lines/genotypes were conducted during two years (2010-2011) at the four treatments and two locations: Pancevo and Zemun Polje. The results showed that the influence of: genotype (G), locality (L), treatment (T) and GxL, GxT, YxL, YxT, LXT, GxYxT, GxLxT, YxLxT, GxYxLxT interaction, on maize yield were significant (p lt 0.01). The share of genotype for maize grain yield in the total phenotypic variance was 21.16%, the aggregate share of the years and the locality was 6.10%, the treatment was 18.22%, and the total interaction was 54.52%. The AMMI analysis of the main components of IPCA1 and IPCA2 for the interaction of GxL and GxT shows that the first major component, IPCA1, comprises 100% of the sum of the squared interaction GxL and showed a statistically significant effect. The results also show that the sums of the squares of the first and second major components (PC1 and PC2) constitute 100% of the sum of the squared interaction GxL. The IPCA1 share in the GxT interaction was 47.39% and the IPC2 was 37.94%. IPC1 and IPC2 for this interaction was 85.33%. A high level of IPC2 indicates a significant treatment effect. The results of AMI analysis show that there is a significant difference between the genotype reactions to different ecological conditions for investigated factors. It also provided better insight in specific association between maize grain yield, locality, treatment and meteorological variables. Among the tested maize lines/genotypes, L-5, L-4 and L-6 could be separated as highest yielding genotypes, however L-5 could be recommended for further breeding program and in large-scale seed production due to its stable and high yielding performance.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment stability of maize lines yield by GGE-biplot analysis",
volume = "50",
number = "3",
pages = "755-770",
doi = "10.2298/GENSR1803755B"
}
Božović, D., Zivanović, T., Popović, V., Tatić, M., Gospavić, Z., Miloradović, Z., Dokić, M.,& Stanković, G.. (2018). Assessment stability of maize lines yield by GGE-biplot analysis. in Genetika
Društvo genetičara Srbije, Beograd., 50(3), 755-770.
https://doi.org/10.2298/GENSR1803755B
Božović D, Zivanović T, Popović V, Tatić M, Gospavić Z, Miloradović Z, Dokić M, Stanković G. Assessment stability of maize lines yield by GGE-biplot analysis. in Genetika. 2018;50(3):755-770.
doi:10.2298/GENSR1803755B .
Božović, Dragan, Zivanović, Tomislav, Popović, Vera, Tatić, Mladen, Gospavić, Zagorka, Miloradović, Zoran, Dokić, Milorad, Stanković, Goran, "Assessment stability of maize lines yield by GGE-biplot analysis" in Genetika, 50, no. 3 (2018):755-770,
https://doi.org/10.2298/GENSR1803755B . .
14
7
15

Comparison of statistical methods for genetic similarity evaluation of maize inbred lines

Nikolić, Ana; Kostadinović, Marija; Vančetović, Jelena; Stanković, Goran; Ignjatović-Micić, Dragana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2017)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Ignjatović-Micić, Dragana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/679
AB  - Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index.
AB  - Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Comparison of statistical methods for genetic similarity evaluation of maize inbred lines
T1  - Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza
VL  - 54
IS  - 1
SP  - 25
EP  - 30
DO  - 10.5937/ratpov54-12174
ER  - 
@article{
author = "Nikolić, Ana and Kostadinović, Marija and Vančetović, Jelena and Stanković, Goran and Ignjatović-Micić, Dragana",
year = "2017",
abstract = "Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index., Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines, Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza",
volume = "54",
number = "1",
pages = "25-30",
doi = "10.5937/ratpov54-12174"
}
Nikolić, A., Kostadinović, M., Vančetović, J., Stanković, G.,& Ignjatović-Micić, D.. (2017). Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(1), 25-30.
https://doi.org/10.5937/ratpov54-12174
Nikolić A, Kostadinović M, Vančetović J, Stanković G, Ignjatović-Micić D. Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo. 2017;54(1):25-30.
doi:10.5937/ratpov54-12174 .
Nikolić, Ana, Kostadinović, Marija, Vančetović, Jelena, Stanković, Goran, Ignjatović-Micić, Dragana, "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines" in Ratarstvo i povrtarstvo, 54, no. 1 (2017):25-30,
https://doi.org/10.5937/ratpov54-12174 . .
1

Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits

Kostadinović, Marija; Ignjatović-Micić, Dragana; Ristić, Danijela; Mladenović Drinić, Snežana; Vančetović, Jelena; Božinović, Sofija; Stanković, Goran

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Stanković, Goran
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/621
AB  - Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.
PB  - Public Library Science, San Francisco
T2  - PLOS ONE
T1  - Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167635
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Ristić, Danijela and Mladenović Drinić, Snežana and Vančetović, Jelena and Božinović, Sofija and Stanković, Goran",
year = "2016",
abstract = "Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.",
publisher = "Public Library Science, San Francisco",
journal = "PLOS ONE",
title = "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167635"
}
Kostadinović, M., Ignjatović-Micić, D., Ristić, D., Mladenović Drinić, S., Vančetović, J., Božinović, S.,& Stanković, G.. (2016). Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167635
Kostadinović M, Ignjatović-Micić D, Ristić D, Mladenović Drinić S, Vančetović J, Božinović S, Stanković G. Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE. 2016;11(12).
doi:10.1371/journal.pone.0167635 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Ristić, Danijela, Mladenović Drinić, Snežana, Vančetović, Jelena, Božinović, Sofija, Stanković, Goran, "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits" in PLOS ONE, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167635 . .
1
13
7
13

Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima

Stanković, Goran

(Универзитет у Београду, Пољопривредни факултет, 2016)

TY  - THES
AU  - Stanković, Goran
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3772
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:12683/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48149519
UR  - http://nardus.mpn.gov.rs/123456789/6426
UR  - http://rik.mrizp.rs/handle/123456789/761
AB  - U radu je proučavana divergentnost sintetičkih populacija, roditeljskih linija i linija potomstava analizom srednjih vrednosti njihovih deset osobina i primenom SSR markera. Za istraţivanja su korišćene tri sintetičke populacije per se i njihove S1 familije, deset roditeljskih linija i jedanaest linija potomstava. Sintetička populacija Syn ZP je nastala dialelnim ukrštanjem devet inbred linija poreklom iz lokalnih sorti sa Zapadnog Balkana, Syn ZP/USA iz ukrštanja linija lokalnog porekla sa linijom poreklom iz BSS sintetika, a Syn FS rekombinacijom predhodne dve populacije. Linije potomstva su poreklom iz sintetičkih populacija Syn ZP i Syn ZP/USA.Na osnovu rezultata istraţivanja utvrĎeno je da su se S1 familije tri sintetičke populacije značajno razlikovale po srednjim vrednostima ispitivanih osobina. Varijabilnost i heritabilnost ispitivanih osobina je povećana nakon unošenja strane germplazme, kao i nakon rekombinacije sintetičkih populacija, a naročito je izraţeno povećanje genetičke dobiti od selekcije. Genetički koeficijenti korelacija su pokazali da je kod sve tri populacije najveći uticaj na prinos zrna imala duţina klipa, a najmanji masa 1000 zrna. Rezultati analize glavnih komponenti su pokazali da je duţina klipa, zajedno sa brojem zrna u redu, imala najveći indirektan uticaj na prinos zrna kod S1 familija sve tri sintetičke populacije. Inkorporacija strane germplazme, kao i rekombinacija sintetičkih populacija, su doprinele stvaranju nove varijabilnosti ispitivanog genetičkog materijala i poboljšanju srednjih vrednosti ispitivanih osobina.Rezultati istraţivanja sintetičkih populacija per se, roditeljskih komponenti i linija potomstava su ukazali da su selekcionisane linije imale bolje srednje vrednosti za ispitivane osobine od roditeljskih linija. Koeficijenti korelacija su bili pribliţno isti kod linija roditelja i linija potomstava, s tim da je uočeno povećanje uticaja mase zrna naprinos, u odnosu na S1 familije. Na osnovu ovih rezultata odabrano je nekoliko linija potomstava kod kojih je zabeleţeno poboljšanje osobina u odnosu na roditelje i koje mogu biti perspektivne za dalju selekciju...
AB  - Divergence of synthetic populations, parental and progeny inbred lines has been studied using both mean values of ten phenotypic traits and SSR markers. The research was performed on three synthetic populations per se and their S1 families, ten parental and 11 progeny inbred lines. Syn ZP synthetic population was obtained by diallel crossing of nine inbreds that originated from local, West Balkans, landraces, Syn ZP/USA by crossing inbreds of local origin with an inbred from BSS synthetic and Syn FS by recombination of the two previous populations. Progeny inbred lines were obtained from Syn ZP and Syn ZP/USA.The results revealed that S1 families of the three synthetics were significantly different in the mean values of the analysed traits. Variability and heritability of these traits has been increased after introduction of exotic germplasm as well as after recombination of the synthetic populations and genetic gain was particularly expressed. Genetic correlation coefficients showed that ear length had the highest and 1000 kernel weight the lowest impact on grain yield. Principal component analysis showed that ear length, together with kernel number per row, had the highest indirect impact on grain yield of S1 families considering all three synthetic populations. Incorporation of exotic germplasm, as well as recombination of the synthetic populations, contributed to the generation of new variability in the genetic material and improvement of mean values of the analysed traits.Analyses of synthetic populations per se, parental components and progeny inbreds indicated that new inbred lines had better mean values of the traits compared to parental inbreds. Correlation coefficients were approximately the same within parental inbreds and within progeny inbreds, except that increase of kernel weight impact ongrain yield in regard to S1 families was observed. Based on the presented results of the research, several progeny inbreds have been chosen, which have better trait performances in comparison to parents and could be perspective for further selection...
PB  - Универзитет у Београду, Пољопривредни факултет
T2  - Универзитет у Београду
T1  - Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima
T1  - Divergence of maize synthetic populations and their progenies determined by phenotypic and molecular markers
UR  - https://hdl.handle.net/21.15107/rcub_nardus_6426
ER  - 
@phdthesis{
author = "Stanković, Goran",
year = "2016",
abstract = "U radu je proučavana divergentnost sintetičkih populacija, roditeljskih linija i linija potomstava analizom srednjih vrednosti njihovih deset osobina i primenom SSR markera. Za istraţivanja su korišćene tri sintetičke populacije per se i njihove S1 familije, deset roditeljskih linija i jedanaest linija potomstava. Sintetička populacija Syn ZP je nastala dialelnim ukrštanjem devet inbred linija poreklom iz lokalnih sorti sa Zapadnog Balkana, Syn ZP/USA iz ukrštanja linija lokalnog porekla sa linijom poreklom iz BSS sintetika, a Syn FS rekombinacijom predhodne dve populacije. Linije potomstva su poreklom iz sintetičkih populacija Syn ZP i Syn ZP/USA.Na osnovu rezultata istraţivanja utvrĎeno je da su se S1 familije tri sintetičke populacije značajno razlikovale po srednjim vrednostima ispitivanih osobina. Varijabilnost i heritabilnost ispitivanih osobina je povećana nakon unošenja strane germplazme, kao i nakon rekombinacije sintetičkih populacija, a naročito je izraţeno povećanje genetičke dobiti od selekcije. Genetički koeficijenti korelacija su pokazali da je kod sve tri populacije najveći uticaj na prinos zrna imala duţina klipa, a najmanji masa 1000 zrna. Rezultati analize glavnih komponenti su pokazali da je duţina klipa, zajedno sa brojem zrna u redu, imala najveći indirektan uticaj na prinos zrna kod S1 familija sve tri sintetičke populacije. Inkorporacija strane germplazme, kao i rekombinacija sintetičkih populacija, su doprinele stvaranju nove varijabilnosti ispitivanog genetičkog materijala i poboljšanju srednjih vrednosti ispitivanih osobina.Rezultati istraţivanja sintetičkih populacija per se, roditeljskih komponenti i linija potomstava su ukazali da su selekcionisane linije imale bolje srednje vrednosti za ispitivane osobine od roditeljskih linija. Koeficijenti korelacija su bili pribliţno isti kod linija roditelja i linija potomstava, s tim da je uočeno povećanje uticaja mase zrna naprinos, u odnosu na S1 familije. Na osnovu ovih rezultata odabrano je nekoliko linija potomstava kod kojih je zabeleţeno poboljšanje osobina u odnosu na roditelje i koje mogu biti perspektivne za dalju selekciju..., Divergence of synthetic populations, parental and progeny inbred lines has been studied using both mean values of ten phenotypic traits and SSR markers. The research was performed on three synthetic populations per se and their S1 families, ten parental and 11 progeny inbred lines. Syn ZP synthetic population was obtained by diallel crossing of nine inbreds that originated from local, West Balkans, landraces, Syn ZP/USA by crossing inbreds of local origin with an inbred from BSS synthetic and Syn FS by recombination of the two previous populations. Progeny inbred lines were obtained from Syn ZP and Syn ZP/USA.The results revealed that S1 families of the three synthetics were significantly different in the mean values of the analysed traits. Variability and heritability of these traits has been increased after introduction of exotic germplasm as well as after recombination of the synthetic populations and genetic gain was particularly expressed. Genetic correlation coefficients showed that ear length had the highest and 1000 kernel weight the lowest impact on grain yield. Principal component analysis showed that ear length, together with kernel number per row, had the highest indirect impact on grain yield of S1 families considering all three synthetic populations. Incorporation of exotic germplasm, as well as recombination of the synthetic populations, contributed to the generation of new variability in the genetic material and improvement of mean values of the analysed traits.Analyses of synthetic populations per se, parental components and progeny inbreds indicated that new inbred lines had better mean values of the traits compared to parental inbreds. Correlation coefficients were approximately the same within parental inbreds and within progeny inbreds, except that increase of kernel weight impact ongrain yield in regard to S1 families was observed. Based on the presented results of the research, several progeny inbreds have been chosen, which have better trait performances in comparison to parents and could be perspective for further selection...",
publisher = "Универзитет у Београду, Пољопривредни факултет",
journal = "Универзитет у Београду",
title = "Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima, Divergence of maize synthetic populations and their progenies determined by phenotypic and molecular markers",
url = "https://hdl.handle.net/21.15107/rcub_nardus_6426"
}
Stanković, G.. (2016). Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima. in Универзитет у Београду
Универзитет у Београду, Пољопривредни факултет..
https://hdl.handle.net/21.15107/rcub_nardus_6426
Stanković G. Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima. in Универзитет у Београду. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_6426 .
Stanković, Goran, "Utvrđivanje divergentnosti sintetičkih populacija kukuruza i njihovog potomstva fenotipskim i molekularnim markerima" in Универзитет у Београду (2016),
https://hdl.handle.net/21.15107/rcub_nardus_6426 .

Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value

Radenović, Čedomir; Delić, Nenad; Sečanski, Mile; Jovanović, Života; Stanković, Goran; Popović, Aleksandar

(2015)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Delić, Nenad
AU  - Sečanski, Mile
AU  - Jovanović, Života
AU  - Stanković, Goran
AU  - Popović, Aleksandar
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/601
AB  - This paper presents results of several different studies that confirm the hypothesis that maize inbred lines rich in proteins and with exceptional nutritive values can be bred. This is also supported by a medicinal standpoint of programmed need for maize-based food and feed. With such an experimental approach, the maize inbred lines ZPPL 146 and ZPPL 159 and hybrids derived from them (ZP 633, ZP 735 and ZP 737) rich in proteins have been systematically tested. Based on concrete results the following we can be conclude as follows. Selected maize inbred lines ZPPL 146 and ZPPL 159, rich in carotenoids, yellow pigments, also have significant amounts of other relevant bioactive compounds. Observed inbred lines have erect top leaves and are classified into a group of maize inbreds with significant properties of the photosynthesis model, are resistant to high temperatures and tolerant to drought. Spectral bands pointing to conformational characteristics of molecules of carotenoids but also of other compounds (phosphates, glutens and amides III) were established by the resonance Raman spectroscopy method applied to the leaf of the maize inbred line ZPLL 146. Physiological, biochemical and biophysical traits of elite maize inbred lines and their hybrids were observed in this study with a special emphasis on their efficiency of photosynthesis, productivity and suitability for broad use of nutrient values of grain and other essential biogenic matters, first of all pigments that express antioxidative properties. Relevant traits, properties and parameters of observed maize inbred lines that can be used in the process of selection are presented. These prestigious maize inbred lines were used to develop high-yielding and high-quality maize hybrids (ZP 633, ZP 735 and ZP 737) that are recognisable for their quality in human nutrition (children and the elderly), that are confirmed by medical observations related to their use in food and feed. Relevant agronomic and morphological traits of maize hybrids are presented. Moreover, results on grain structure and yields obtained in the regions of south-eastern Europe are also displayed.
T2  - Sel'skokhozyaistvennaya Biologiya
T1  - Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value
VL  - 50
IS  - 5
SP  - 600
EP  - 610
DO  - 10.15389/agrobiology.2015.5.600eng
ER  - 
@article{
author = "Radenović, Čedomir and Delić, Nenad and Sečanski, Mile and Jovanović, Života and Stanković, Goran and Popović, Aleksandar",
year = "2015",
abstract = "This paper presents results of several different studies that confirm the hypothesis that maize inbred lines rich in proteins and with exceptional nutritive values can be bred. This is also supported by a medicinal standpoint of programmed need for maize-based food and feed. With such an experimental approach, the maize inbred lines ZPPL 146 and ZPPL 159 and hybrids derived from them (ZP 633, ZP 735 and ZP 737) rich in proteins have been systematically tested. Based on concrete results the following we can be conclude as follows. Selected maize inbred lines ZPPL 146 and ZPPL 159, rich in carotenoids, yellow pigments, also have significant amounts of other relevant bioactive compounds. Observed inbred lines have erect top leaves and are classified into a group of maize inbreds with significant properties of the photosynthesis model, are resistant to high temperatures and tolerant to drought. Spectral bands pointing to conformational characteristics of molecules of carotenoids but also of other compounds (phosphates, glutens and amides III) were established by the resonance Raman spectroscopy method applied to the leaf of the maize inbred line ZPLL 146. Physiological, biochemical and biophysical traits of elite maize inbred lines and their hybrids were observed in this study with a special emphasis on their efficiency of photosynthesis, productivity and suitability for broad use of nutrient values of grain and other essential biogenic matters, first of all pigments that express antioxidative properties. Relevant traits, properties and parameters of observed maize inbred lines that can be used in the process of selection are presented. These prestigious maize inbred lines were used to develop high-yielding and high-quality maize hybrids (ZP 633, ZP 735 and ZP 737) that are recognisable for their quality in human nutrition (children and the elderly), that are confirmed by medical observations related to their use in food and feed. Relevant agronomic and morphological traits of maize hybrids are presented. Moreover, results on grain structure and yields obtained in the regions of south-eastern Europe are also displayed.",
journal = "Sel'skokhozyaistvennaya Biologiya",
title = "Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value",
volume = "50",
number = "5",
pages = "600-610",
doi = "10.15389/agrobiology.2015.5.600eng"
}
Radenović, Č., Delić, N., Sečanski, M., Jovanović, Ž., Stanković, G.,& Popović, A.. (2015). Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value. in Sel'skokhozyaistvennaya Biologiya, 50(5), 600-610.
https://doi.org/10.15389/agrobiology.2015.5.600eng
Radenović Č, Delić N, Sečanski M, Jovanović Ž, Stanković G, Popović A. Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value. in Sel'skokhozyaistvennaya Biologiya. 2015;50(5):600-610.
doi:10.15389/agrobiology.2015.5.600eng .
Radenović, Čedomir, Delić, Nenad, Sečanski, Mile, Jovanović, Života, Stanković, Goran, Popović, Aleksandar, "Maize (Zea mays L.) inbred lines and hybrids of serbian selection with high efficiency of photosynthesis, rich in pigment content and increased nutritive value" in Sel'skokhozyaistvennaya Biologiya, 50, no. 5 (2015):600-610,
https://doi.org/10.15389/agrobiology.2015.5.600eng . .
1
1

Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence

Radenović, Čedomir; Stanković, Goran; Jovanović, Života; Maksimov, Georgij; Tyutyaev, Evgenij V.; Beljanski, Miloš

(Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux, 2014)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Stanković, Goran
AU  - Jovanović, Života
AU  - Maksimov, Georgij
AU  - Tyutyaev, Evgenij V.
AU  - Beljanski, Miloš
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/542
AB  - In this paper the changes on growth, photosynthesis and water relations were tested by non-invasive fluorescence method. The applications of this method allow to determine some functional properties of prestigious maize inbred lines with erect top leaves. So the temperature dependency of delayed fluorescence intensity maximum of ZPPL 16 is observed at higher temperatures than for ZPPZ 62. This fact correlates with low values of phase transition of activation energy Ea in thylakoid membrane and accompanied by a decrease of the angle and area of the leaf, as well as with the content and the rate of water release from the seed. It seems reasonable to assume that, DF can be applied in breeding and maize hybrid seed production for the estimation of prestigious maize inbred lines and their resistance adaptability to increased and high temperatures, as well as, to drought.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence
VL  - 81
SP  - 208
EP  - 211
DO  - 10.1016/j.plaphy.2014.03.013
ER  - 
@article{
author = "Radenović, Čedomir and Stanković, Goran and Jovanović, Života and Maksimov, Georgij and Tyutyaev, Evgenij V. and Beljanski, Miloš",
year = "2014",
abstract = "In this paper the changes on growth, photosynthesis and water relations were tested by non-invasive fluorescence method. The applications of this method allow to determine some functional properties of prestigious maize inbred lines with erect top leaves. So the temperature dependency of delayed fluorescence intensity maximum of ZPPL 16 is observed at higher temperatures than for ZPPZ 62. This fact correlates with low values of phase transition of activation energy Ea in thylakoid membrane and accompanied by a decrease of the angle and area of the leaf, as well as with the content and the rate of water release from the seed. It seems reasonable to assume that, DF can be applied in breeding and maize hybrid seed production for the estimation of prestigious maize inbred lines and their resistance adaptability to increased and high temperatures, as well as, to drought.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence",
volume = "81",
pages = "208-211",
doi = "10.1016/j.plaphy.2014.03.013"
}
Radenović, Č., Stanković, G., Jovanović, Ž., Maksimov, G., Tyutyaev, E. V.,& Beljanski, M.. (2014). Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 81, 208-211.
https://doi.org/10.1016/j.plaphy.2014.03.013
Radenović Č, Stanković G, Jovanović Ž, Maksimov G, Tyutyaev EV, Beljanski M. Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence. in Plant Physiology and Biochemistry. 2014;81:208-211.
doi:10.1016/j.plaphy.2014.03.013 .
Radenović, Čedomir, Stanković, Goran, Jovanović, Života, Maksimov, Georgij, Tyutyaev, Evgenij V., Beljanski, Miloš, "Detecting the phase transition in thylakoid membrane of maize inbred lines by means of delayed fluorescence" in Plant Physiology and Biochemistry, 81 (2014):208-211,
https://doi.org/10.1016/j.plaphy.2014.03.013 . .
4
3
6

Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line

Kostadinović, Marija; Ignjatović-Micić, Dragana; Stanković, Goran; Vančetović, Jelena; Ristić, Danijela; Mladenović Drinić, Snežana

(Burgas : Sciences events, 2014)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović Drinić, Snežana
PY  - 2014
UR  - http://rik.mrizp.rs/handle/123456789/959
AB  - Quality protein maize (QPM) is the maize with increased levels of two essential amino acids, hard endosperm and good agronomic performances. The recessive opaque2 (o2) mutation increases lysine and tryptophan content in maize grains. It has recently become possible to use marker assisted selection (MAS) to accelerate selection for the o2 allele in QPM breeding process. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The results presented herein are the part of marker assisted backcross breeding program for incorporation of the o2 into maize inbred line. Marker selected o2 recessive homozygous BC2F2 individuals were subjected to the whole genome background selection, which identified five progenies with 83 to 94% recurrent parent genome content. BC2F3 families are being evaluated for desirable agronomic and biochemical traits in replicated trials and the best lines will represent the QPM version of the standard line.
PB  - Burgas : Sciences events
C3  - Journal of international scientific publications: agriculture & food
T1  - Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line
VL  - 2
SP  - 199
EP  - 205
UR  - https://hdl.handle.net/21.15107/rcub_rik_959
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Stanković, Goran and Vančetović, Jelena and Ristić, Danijela and Mladenović Drinić, Snežana",
year = "2014",
abstract = "Quality protein maize (QPM) is the maize with increased levels of two essential amino acids, hard endosperm and good agronomic performances. The recessive opaque2 (o2) mutation increases lysine and tryptophan content in maize grains. It has recently become possible to use marker assisted selection (MAS) to accelerate selection for the o2 allele in QPM breeding process. Maize Research Institute Zemun Polje has a program on developing QPM genotypes for growing in temperate regions. The results presented herein are the part of marker assisted backcross breeding program for incorporation of the o2 into maize inbred line. Marker selected o2 recessive homozygous BC2F2 individuals were subjected to the whole genome background selection, which identified five progenies with 83 to 94% recurrent parent genome content. BC2F3 families are being evaluated for desirable agronomic and biochemical traits in replicated trials and the best lines will represent the QPM version of the standard line.",
publisher = "Burgas : Sciences events",
journal = "Journal of international scientific publications: agriculture & food",
title = "Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line",
volume = "2",
pages = "199-205",
url = "https://hdl.handle.net/21.15107/rcub_rik_959"
}
Kostadinović, M., Ignjatović-Micić, D., Stanković, G., Vančetović, J., Ristić, D.,& Mladenović Drinić, S.. (2014). Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line. in Journal of international scientific publications: agriculture & food
Burgas : Sciences events., 2, 199-205.
https://hdl.handle.net/21.15107/rcub_rik_959
Kostadinović M, Ignjatović-Micić D, Stanković G, Vančetović J, Ristić D, Mladenović Drinić S. Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line. in Journal of international scientific publications: agriculture & food. 2014;2:199-205.
https://hdl.handle.net/21.15107/rcub_rik_959 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Stanković, Goran, Vančetović, Jelena, Ristić, Danijela, Mladenović Drinić, Snežana, "Marker asissted backcrossing for incorporation of the opaque2 gene into a standard maize inbred line" in Journal of international scientific publications: agriculture & food, 2 (2014):199-205,
https://hdl.handle.net/21.15107/rcub_rik_959 .