Lazić-Jančić, Vesna

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  • Lazić-Jančić, Vesna (15)
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Author's Bibliography

Identification of QTLS for yield and drought-related traits in maize: assessment of their causal relationships

Nikolić, Ana; Ignjatović-Micić, Dragana; Dodig, Dejan; Anđelković, Violeta; Lazić-Jančić, Vesna

(Diagnosis Press Ltd, Sofia, 2012)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Dodig, Dejan
AU  - Anđelković, Violeta
AU  - Lazić-Jančić, Vesna
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/427
AB  - Drought is one of the most important factors contributing to crop yield loss. Developing maize varieties with drought tolerance requires exploring the genetic basis of yield and associated agronomic traits. In order to identify QTLs for yield and drought related traits and to determine their relationships, 116 F-3 families of the DTP79xB73 cross were evaluated in field trials. Phenotypic correlations between the traits were calculated using Pearson's coefficient. QTL detection was performed using ANOVA and composite interval mapping (CIM). Out of 64 QTLs identified using CIM for all analyzed traits 26 QTLs were also identified with ANOVA. For all the traits (except for EL, DS2 and RWC1) only a small proportion of phenotypic variation was accounted for by the identified QTL. The significant positive/negative phenotypic correlations between different traits and coincidence of markers with allelic differences in the expected direction show that they may be causally correlated. The above criteria were fullfilled for a subset of QTLs within all the traits. The overlap found between the QTLs for yield and those for the other traits suggests the presence of genes with pleiotropic effects on the investigated traits. Results will be used for further investigation and detection of possible candidate genes for drought tolerance in maize.
PB  - Diagnosis Press Ltd, Sofia
T2  - Biotechnology & Biotechnological Equipment
T1  - Identification of QTLS for yield and drought-related traits in maize: assessment of their causal relationships
VL  - 26
IS  - 3
SP  - 2952
EP  - 2960
DO  - 10.5504/BBEQ.2012.0016
UR  - conv_815
ER  - 
@article{
author = "Nikolić, Ana and Ignjatović-Micić, Dragana and Dodig, Dejan and Anđelković, Violeta and Lazić-Jančić, Vesna",
year = "2012",
abstract = "Drought is one of the most important factors contributing to crop yield loss. Developing maize varieties with drought tolerance requires exploring the genetic basis of yield and associated agronomic traits. In order to identify QTLs for yield and drought related traits and to determine their relationships, 116 F-3 families of the DTP79xB73 cross were evaluated in field trials. Phenotypic correlations between the traits were calculated using Pearson's coefficient. QTL detection was performed using ANOVA and composite interval mapping (CIM). Out of 64 QTLs identified using CIM for all analyzed traits 26 QTLs were also identified with ANOVA. For all the traits (except for EL, DS2 and RWC1) only a small proportion of phenotypic variation was accounted for by the identified QTL. The significant positive/negative phenotypic correlations between different traits and coincidence of markers with allelic differences in the expected direction show that they may be causally correlated. The above criteria were fullfilled for a subset of QTLs within all the traits. The overlap found between the QTLs for yield and those for the other traits suggests the presence of genes with pleiotropic effects on the investigated traits. Results will be used for further investigation and detection of possible candidate genes for drought tolerance in maize.",
publisher = "Diagnosis Press Ltd, Sofia",
journal = "Biotechnology & Biotechnological Equipment",
title = "Identification of QTLS for yield and drought-related traits in maize: assessment of their causal relationships",
volume = "26",
number = "3",
pages = "2952-2960",
doi = "10.5504/BBEQ.2012.0016",
url = "conv_815"
}
Nikolić, A., Ignjatović-Micić, D., Dodig, D., Anđelković, V.,& Lazić-Jančić, V.. (2012). Identification of QTLS for yield and drought-related traits in maize: assessment of their causal relationships. in Biotechnology & Biotechnological Equipment
Diagnosis Press Ltd, Sofia., 26(3), 2952-2960.
https://doi.org/10.5504/BBEQ.2012.0016
conv_815
Nikolić A, Ignjatović-Micić D, Dodig D, Anđelković V, Lazić-Jančić V. Identification of QTLS for yield and drought-related traits in maize: assessment of their causal relationships. in Biotechnology & Biotechnological Equipment. 2012;26(3):2952-2960.
doi:10.5504/BBEQ.2012.0016
conv_815 .
Nikolić, Ana, Ignjatović-Micić, Dragana, Dodig, Dejan, Anđelković, Violeta, Lazić-Jančić, Vesna, "Identification of QTLS for yield and drought-related traits in maize: assessment of their causal relationships" in Biotechnology & Biotechnological Equipment, 26, no. 3 (2012):2952-2960,
https://doi.org/10.5504/BBEQ.2012.0016 .,
conv_815 .
8
5
7

Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance

Denić, Miloje; Ignjatović-Micić, Dragana; Stanković, Goran; Marković, Ksenija; Žilić, Slađana; Lazić-Jančić, Vesna; Chauque, Pedro; Fato, Pedro; Senete, Constantino; Mariote, David; Haag, Wayne

(Društvo genetičara Srbije, Beograd, 2012)

TY  - JOUR
AU  - Denić, Miloje
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Žilić, Slađana
AU  - Lazić-Jančić, Vesna
AU  - Chauque, Pedro
AU  - Fato, Pedro
AU  - Senete, Constantino
AU  - Mariote, David
AU  - Haag, Wayne
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/462
AB  - Due to the low biological value of proteins of common maize, it was reinitiated breeding for high protein quality maize (HQPM) using three genetic systems, namely: opaque-2 gene, endosperm modifier genes and enhancer genes, which are increasing lysine and tryptophan content in opaque-2 background In order to alleviate effect of abiotic and biotic stress factors, the genotypes with tolerance to those factors were included. Genetic resources originating from North, Central and South America, then West, Central and Southern Africa and gene bank of Maize Research Institute 'Zemun Polje' were used. Combining breeding approaches in selection of genetic resources, field plot techniques and laboratory analysis, it was created large number of early QPM varieties, inbred lines and hybrids with modified endosperm and high yield potential under poor and good growing conditions. Created lines exhibited high combining ability in conventional and non-conventional hybrids. Yield trials showed that QPM hybrids are competing with commercial hybrids of common maize.
AB  - Zbog niske biološke vrednosti proteina zrna standardnog tipa kukuruza se pristupilo stvaranju sorata i linija kukuruza visokog kvaliteta proteina (VKP) korišćenjem tri genetička sistema: opaque-2 gena, gena modifikatora endosperma i gena enhansera koji povećavaju sadržaj lizina i triptofana u opaque-2 osnovi. Da bi se istovremeno ublažilo i delovanje abiotskih i biotskih faktora stresa uključeni su i genotipovi sa tolerantnošću na ove faktore. Korišćeni su genetički resursi poreklom iz Severne, Centralne i Južne Amerike, zatim Zapadne, Centralne i Južne Afrike i resursi iz banke gena Instituta za kukuruz 'Zemun polje'. Kombinovanjem selekcionih pristupa u izboru genetičkih resursa, tehnika u poljskim i laboratorijskim uslovima stvoren je veliki broj ranih sorata, inbred linija i hibrida VKP, modifikikovanog endosperma i visokog potencijala rodnosti pod lošim i normalnim uslovima gajenja. Takođe, stvorene inbred linije su ispoljile visoku kombinacionu sposobnost u nekonvencionalnim i konvencionalnim hibridima. Poljski ogledi su pokazali da VKP hibridi konkurišu po prinosu najboljim komercijalnim hibridima standardnog tipa kukuruza.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance
T1  - Značaj genetičkih resursa iz različitih geografskih i klimatskih regiona u simultanom oplemenjivanju kukuruza na visok kvalitet proteina (VKP) i tolerantnost na stres
VL  - 44
IS  - 1
SP  - 13
EP  - 23
DO  - 10.2298/GENSR1201013D
UR  - conv_459
ER  - 
@article{
author = "Denić, Miloje and Ignjatović-Micić, Dragana and Stanković, Goran and Marković, Ksenija and Žilić, Slađana and Lazić-Jančić, Vesna and Chauque, Pedro and Fato, Pedro and Senete, Constantino and Mariote, David and Haag, Wayne",
year = "2012",
abstract = "Due to the low biological value of proteins of common maize, it was reinitiated breeding for high protein quality maize (HQPM) using three genetic systems, namely: opaque-2 gene, endosperm modifier genes and enhancer genes, which are increasing lysine and tryptophan content in opaque-2 background In order to alleviate effect of abiotic and biotic stress factors, the genotypes with tolerance to those factors were included. Genetic resources originating from North, Central and South America, then West, Central and Southern Africa and gene bank of Maize Research Institute 'Zemun Polje' were used. Combining breeding approaches in selection of genetic resources, field plot techniques and laboratory analysis, it was created large number of early QPM varieties, inbred lines and hybrids with modified endosperm and high yield potential under poor and good growing conditions. Created lines exhibited high combining ability in conventional and non-conventional hybrids. Yield trials showed that QPM hybrids are competing with commercial hybrids of common maize., Zbog niske biološke vrednosti proteina zrna standardnog tipa kukuruza se pristupilo stvaranju sorata i linija kukuruza visokog kvaliteta proteina (VKP) korišćenjem tri genetička sistema: opaque-2 gena, gena modifikatora endosperma i gena enhansera koji povećavaju sadržaj lizina i triptofana u opaque-2 osnovi. Da bi se istovremeno ublažilo i delovanje abiotskih i biotskih faktora stresa uključeni su i genotipovi sa tolerantnošću na ove faktore. Korišćeni su genetički resursi poreklom iz Severne, Centralne i Južne Amerike, zatim Zapadne, Centralne i Južne Afrike i resursi iz banke gena Instituta za kukuruz 'Zemun polje'. Kombinovanjem selekcionih pristupa u izboru genetičkih resursa, tehnika u poljskim i laboratorijskim uslovima stvoren je veliki broj ranih sorata, inbred linija i hibrida VKP, modifikikovanog endosperma i visokog potencijala rodnosti pod lošim i normalnim uslovima gajenja. Takođe, stvorene inbred linije su ispoljile visoku kombinacionu sposobnost u nekonvencionalnim i konvencionalnim hibridima. Poljski ogledi su pokazali da VKP hibridi konkurišu po prinosu najboljim komercijalnim hibridima standardnog tipa kukuruza.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance, Značaj genetičkih resursa iz različitih geografskih i klimatskih regiona u simultanom oplemenjivanju kukuruza na visok kvalitet proteina (VKP) i tolerantnost na stres",
volume = "44",
number = "1",
pages = "13-23",
doi = "10.2298/GENSR1201013D",
url = "conv_459"
}
Denić, M., Ignjatović-Micić, D., Stanković, G., Marković, K., Žilić, S., Lazić-Jančić, V., Chauque, P., Fato, P., Senete, C., Mariote, D.,& Haag, W.. (2012). Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance. in Genetika
Društvo genetičara Srbije, Beograd., 44(1), 13-23.
https://doi.org/10.2298/GENSR1201013D
conv_459
Denić M, Ignjatović-Micić D, Stanković G, Marković K, Žilić S, Lazić-Jančić V, Chauque P, Fato P, Senete C, Mariote D, Haag W. Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance. in Genetika. 2012;44(1):13-23.
doi:10.2298/GENSR1201013D
conv_459 .
Denić, Miloje, Ignjatović-Micić, Dragana, Stanković, Goran, Marković, Ksenija, Žilić, Slađana, Lazić-Jančić, Vesna, Chauque, Pedro, Fato, Pedro, Senete, Constantino, Mariote, David, Haag, Wayne, "Role of genetic resources from different geographic and climatic regions in simultaneous breeding for high quality protein maize (HQPM) and stress tolerance" in Genetika, 44, no. 1 (2012):13-23,
https://doi.org/10.2298/GENSR1201013D .,
conv_459 .
3
3
3

Kernel modifications and tryptophan content in QPM segregating generations

Ignjatović-Micić, Dragana; Stanković, Goran; Marković, Ksenija; Mladenović-Drinić, Snežana; Lazić-Jančić, Vesna; Denić, Miloje

(Društvo genetičara Srbije, Beograd, 2010)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Mladenović-Drinić, Snežana
AU  - Lazić-Jančić, Vesna
AU  - Denić, Miloje
PY  - 2010
UR  - http://rik.mrizp.rs/handle/123456789/303
AB  - Maize has poor nutritional value due to deficiency of two essential amino acids - tryptophan and lysine. Although recessive opaque2 (o2) mutation significantly increases their content in the endosperm, incorporation of opaque2 into high yielding cultivars was not commercially successful, because of its numerous agronomic and processing problems due to soft endosperm. Quality protein maize - QPM has lately been introduced as opaque2 maize with improved endosperm hardness and improved agronomic traits, but mostly within tropical and subtropical germplasm. The ongoing breeding project at MRI includes improvement of MRI opaque2 lines and conversion of standard lines to QPM germplasm. The main selection steps in QPM breeding involve assessing kernel modifications and tryptophan level in each generation. Herein, we present the results of the analysis for these traits on F3 and BC1F1 generations of QPM x opaque2, opaque2 x QPM and standard lines x QPM crosses. The results showed that the majority the genotypes had kernel types 2 and 3 (good modifications). The whole grain tryptophan content in F3 and BC1F1 genotypes of crosses between QPM and opaque2 germplasm was at the quality protein level, with a few exceptions. All BC1F1 genotypes of standard lines x QPM had tryptophan content in the range of normal maize, while majority of F3 genotypes had tryptophan content at level of QPM. The progeny (with increased tryptophan levels) of QPM and opaque2 crosses had significantly higher tryptophan content compared to the progeny of crosses between standard and QPM lines - 0.098 to 0.114 and 0.080, respectively. All genotypes that had poorly modified kernels and/or low tryptophan content will be discarded from further breeding.
AB  - Iako recesivna opaque2 (o2) mutacija značajno povećava sadržaj lizina i triptofana u zrnu kukuruza, inkorporacija opaque2 u visoko prinosne genotipove nije uspela, zbog brojnih problema koji su se javili kao posledica mekog endosperma. Kukuruz visokog kvaliteta proteina (QPM) je opaque2 kukuruz sa poboljšanom tvrdoćom zrna i dobrim agronomskim performansama, stvoren prvenstveno od tropske i subtropske germplazme. U Institutu za kukuruz je u toku projekat koji za cilj ima poboljšanje sopstvenih opaque2 linija i prevođenje standardnih linija u QPM kukuruz. U ovom radu predstavljamo rezultate analiza modifikacije zrna i sadržaja triptofana u F3 i BC1F1 generacijama ukrštanja QPM x opaque2, opaque2 x QPM i standardne linije x QPM. Većina genotipova je imala zrno tipa 2 i tipa3 (dobre modifikacije). Sadržaj triptofana u celom zrnu genotipova ukrštanja QPM i opaque2 linija je bio na nivou karakterističnom za QPM, sa nekoliko izuzetaka. Sadržaj triptofana u potomstvu ukrštanja standardne linije x QPM je bio povećan kod većine F3 genotipova. Potomstvo (sa povećanim sadržajem triptofana) ukrštanja QPM i opaque2 linija je imalo znatno veći sadržaj ove aminokiseline u odnosu na potostvo ukrštanja standardne linije x QPM - - od 0.098 do 0.114 prema 0.080. Genotipovi sa lošim modifikacija zrna i/ili niskim sadržajem triptofana ce biti eleminisani iz daljeg procesa selekcije.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Kernel modifications and tryptophan content in QPM segregating generations
T1  - Modifikacija zrna i sadržaj triptofana u segregirajućim generacijama kukuruza visokog kvaliteta proteina
VL  - 42
IS  - 2
SP  - 267
EP  - 277
DO  - 10.2298/GENSR1002267M
UR  - conv_444
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Stanković, Goran and Marković, Ksenija and Mladenović-Drinić, Snežana and Lazić-Jančić, Vesna and Denić, Miloje",
year = "2010",
abstract = "Maize has poor nutritional value due to deficiency of two essential amino acids - tryptophan and lysine. Although recessive opaque2 (o2) mutation significantly increases their content in the endosperm, incorporation of opaque2 into high yielding cultivars was not commercially successful, because of its numerous agronomic and processing problems due to soft endosperm. Quality protein maize - QPM has lately been introduced as opaque2 maize with improved endosperm hardness and improved agronomic traits, but mostly within tropical and subtropical germplasm. The ongoing breeding project at MRI includes improvement of MRI opaque2 lines and conversion of standard lines to QPM germplasm. The main selection steps in QPM breeding involve assessing kernel modifications and tryptophan level in each generation. Herein, we present the results of the analysis for these traits on F3 and BC1F1 generations of QPM x opaque2, opaque2 x QPM and standard lines x QPM crosses. The results showed that the majority the genotypes had kernel types 2 and 3 (good modifications). The whole grain tryptophan content in F3 and BC1F1 genotypes of crosses between QPM and opaque2 germplasm was at the quality protein level, with a few exceptions. All BC1F1 genotypes of standard lines x QPM had tryptophan content in the range of normal maize, while majority of F3 genotypes had tryptophan content at level of QPM. The progeny (with increased tryptophan levels) of QPM and opaque2 crosses had significantly higher tryptophan content compared to the progeny of crosses between standard and QPM lines - 0.098 to 0.114 and 0.080, respectively. All genotypes that had poorly modified kernels and/or low tryptophan content will be discarded from further breeding., Iako recesivna opaque2 (o2) mutacija značajno povećava sadržaj lizina i triptofana u zrnu kukuruza, inkorporacija opaque2 u visoko prinosne genotipove nije uspela, zbog brojnih problema koji su se javili kao posledica mekog endosperma. Kukuruz visokog kvaliteta proteina (QPM) je opaque2 kukuruz sa poboljšanom tvrdoćom zrna i dobrim agronomskim performansama, stvoren prvenstveno od tropske i subtropske germplazme. U Institutu za kukuruz je u toku projekat koji za cilj ima poboljšanje sopstvenih opaque2 linija i prevođenje standardnih linija u QPM kukuruz. U ovom radu predstavljamo rezultate analiza modifikacije zrna i sadržaja triptofana u F3 i BC1F1 generacijama ukrštanja QPM x opaque2, opaque2 x QPM i standardne linije x QPM. Većina genotipova je imala zrno tipa 2 i tipa3 (dobre modifikacije). Sadržaj triptofana u celom zrnu genotipova ukrštanja QPM i opaque2 linija je bio na nivou karakterističnom za QPM, sa nekoliko izuzetaka. Sadržaj triptofana u potomstvu ukrštanja standardne linije x QPM je bio povećan kod većine F3 genotipova. Potomstvo (sa povećanim sadržajem triptofana) ukrštanja QPM i opaque2 linija je imalo znatno veći sadržaj ove aminokiseline u odnosu na potostvo ukrštanja standardne linije x QPM - - od 0.098 do 0.114 prema 0.080. Genotipovi sa lošim modifikacija zrna i/ili niskim sadržajem triptofana ce biti eleminisani iz daljeg procesa selekcije.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Kernel modifications and tryptophan content in QPM segregating generations, Modifikacija zrna i sadržaj triptofana u segregirajućim generacijama kukuruza visokog kvaliteta proteina",
volume = "42",
number = "2",
pages = "267-277",
doi = "10.2298/GENSR1002267M",
url = "conv_444"
}
Ignjatović-Micić, D., Stanković, G., Marković, K., Mladenović-Drinić, S., Lazić-Jančić, V.,& Denić, M.. (2010). Kernel modifications and tryptophan content in QPM segregating generations. in Genetika
Društvo genetičara Srbije, Beograd., 42(2), 267-277.
https://doi.org/10.2298/GENSR1002267M
conv_444
Ignjatović-Micić D, Stanković G, Marković K, Mladenović-Drinić S, Lazić-Jančić V, Denić M. Kernel modifications and tryptophan content in QPM segregating generations. in Genetika. 2010;42(2):267-277.
doi:10.2298/GENSR1002267M
conv_444 .
Ignjatović-Micić, Dragana, Stanković, Goran, Marković, Ksenija, Mladenović-Drinić, Snežana, Lazić-Jančić, Vesna, Denić, Miloje, "Kernel modifications and tryptophan content in QPM segregating generations" in Genetika, 42, no. 2 (2010):267-277,
https://doi.org/10.2298/GENSR1002267M .,
conv_444 .
2
4
5

Variability analysis of normal and opaque2 maize inbred lines

Ignjatović-Micić, Dragana; Marković, Ksenija; Ristić, Danijela; Mladenović-Drinić, Snežana; Stanković, Slavica; Lazić-Jančić, Vesna; Denić, Miloje

(Društvo genetičara Srbije, Beograd, 2009)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Marković, Ksenija
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
AU  - Stanković, Slavica
AU  - Lazić-Jančić, Vesna
AU  - Denić, Miloje
PY  - 2009
UR  - http://rik.mrizp.rs/handle/123456789/287
AB  - Nutritional value of maize is poor due to deficiency of two essential amino acids - tryptophan and lysine. It was shown than opaque2 (o2) mutations can nearly double the lysine and tryptophan content of the endosperm compared with the normal type. Maize Research Institute Gene bank maintains a collection of opaque2 inbred lines developed in the seventies, primarily based on kernel hardness and analytical methods. In order to describe these lines in more detail they were analyzed for tryptophan content and subjected to SSR analysis with opaque2 markers, a marker for endosperm hardness modifier gene and the most significant amino acid modifier markers. Also, a pathogenicity test for inbred lines tolerance to Fusarium spp., which is one of the most important maize pathogens in our region and a causer of maize stalk, root and ear rot, was performed. Differences in tryptophan content between normal and opaque2 lines were significant. All primers used for distinguishing alleles between normal and opaqu2 inbred lines gave positive results. Each genotype gave a specific allelic pattern with amino acid modifier gene primers, without any obvious correspondence to the tryptophan content. Phythopathogenicity test showed on average higher susceptibility to Fusarium graminearum of opaque2 genotypes. These results gave an insight into the applicability of the methods in describing opaque2 lines to be converted into quality protein maize QPM - genotype in which opaque2 has been incorporated along with associated modifiers.
AB  - Kukuruz ima nisku hranjivu vrednost zbog nedostatka dve esencijalne amino kiseline - triptofana i lizina. Utvrđeno je da opaque2 (o2) mutacija može skoro udvostručiti sadržaj lizina i triptofana u endospermu kukuruza. Banka gena instituta za kukuruz 'Zemun Polje' poseduje kolekciju opaque2 inbred linija razvijenih tokom sedamdesetih godina, prvenstveno na osnovu tvrdoće zrna. Sa ciljem detaljnijeg opisa ovih linija urađena je analiza sadržaja triptofana i SSR analiza sa opaque2 markerima, markerom za gen modifikator tvrdoće endosperma i najsignifikantnijim markerima za gene modifikatore amino kiselina. Takođe je urađen test fitopatogenosti radi utvrđivanja osetljivosti opaque2 genotipova na Fusarium gramaenarum, jednog od najvažnijih prouzrokovača truleži stabla, korena i klipa kukuruza u našem regionu. Utvrđene su značajne razlike u sadržaju triptofana između normalnih i opaque2 linija kukuruza. Svi SSR prajmeri korišćeni za razlikovanje normalnih i opaque2 linija su dali pozitivne rezultate. Svaki od analiziranih genotipova je dao specifičnu alelnu sliku sa markerima za gene modifikatore amino kiselina, bez očigledne saglasnosti sa sadržajem triptofana. Test fitopatogenosti je, u proseku, pokazao veću osetljivost opaque2 linija na Fusarium graminearum. Ovi rezultati su dali uvid u primenjivost navedenih metoda za opisivanje opaque2 linija radi njihovog konvertovanja u kukuruz visokog kvaliteta proteina (QPM) - genotipa u koji se opaque2 inkorporira zajedno sa odgovarajućim genima modifikatorima.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Variability analysis of normal and opaque2 maize inbred lines
T1  - Analiza varijabilnosti normalnih i opaque2 inbred linija kukuruza
VL  - 41
IS  - 1
SP  - 81
EP  - 93
DO  - 10.2298/GENSR0901081I
UR  - conv_430
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Marković, Ksenija and Ristić, Danijela and Mladenović-Drinić, Snežana and Stanković, Slavica and Lazić-Jančić, Vesna and Denić, Miloje",
year = "2009",
abstract = "Nutritional value of maize is poor due to deficiency of two essential amino acids - tryptophan and lysine. It was shown than opaque2 (o2) mutations can nearly double the lysine and tryptophan content of the endosperm compared with the normal type. Maize Research Institute Gene bank maintains a collection of opaque2 inbred lines developed in the seventies, primarily based on kernel hardness and analytical methods. In order to describe these lines in more detail they were analyzed for tryptophan content and subjected to SSR analysis with opaque2 markers, a marker for endosperm hardness modifier gene and the most significant amino acid modifier markers. Also, a pathogenicity test for inbred lines tolerance to Fusarium spp., which is one of the most important maize pathogens in our region and a causer of maize stalk, root and ear rot, was performed. Differences in tryptophan content between normal and opaque2 lines were significant. All primers used for distinguishing alleles between normal and opaqu2 inbred lines gave positive results. Each genotype gave a specific allelic pattern with amino acid modifier gene primers, without any obvious correspondence to the tryptophan content. Phythopathogenicity test showed on average higher susceptibility to Fusarium graminearum of opaque2 genotypes. These results gave an insight into the applicability of the methods in describing opaque2 lines to be converted into quality protein maize QPM - genotype in which opaque2 has been incorporated along with associated modifiers., Kukuruz ima nisku hranjivu vrednost zbog nedostatka dve esencijalne amino kiseline - triptofana i lizina. Utvrđeno je da opaque2 (o2) mutacija može skoro udvostručiti sadržaj lizina i triptofana u endospermu kukuruza. Banka gena instituta za kukuruz 'Zemun Polje' poseduje kolekciju opaque2 inbred linija razvijenih tokom sedamdesetih godina, prvenstveno na osnovu tvrdoće zrna. Sa ciljem detaljnijeg opisa ovih linija urađena je analiza sadržaja triptofana i SSR analiza sa opaque2 markerima, markerom za gen modifikator tvrdoće endosperma i najsignifikantnijim markerima za gene modifikatore amino kiselina. Takođe je urađen test fitopatogenosti radi utvrđivanja osetljivosti opaque2 genotipova na Fusarium gramaenarum, jednog od najvažnijih prouzrokovača truleži stabla, korena i klipa kukuruza u našem regionu. Utvrđene su značajne razlike u sadržaju triptofana između normalnih i opaque2 linija kukuruza. Svi SSR prajmeri korišćeni za razlikovanje normalnih i opaque2 linija su dali pozitivne rezultate. Svaki od analiziranih genotipova je dao specifičnu alelnu sliku sa markerima za gene modifikatore amino kiselina, bez očigledne saglasnosti sa sadržajem triptofana. Test fitopatogenosti je, u proseku, pokazao veću osetljivost opaque2 linija na Fusarium graminearum. Ovi rezultati su dali uvid u primenjivost navedenih metoda za opisivanje opaque2 linija radi njihovog konvertovanja u kukuruz visokog kvaliteta proteina (QPM) - genotipa u koji se opaque2 inkorporira zajedno sa odgovarajućim genima modifikatorima.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Variability analysis of normal and opaque2 maize inbred lines, Analiza varijabilnosti normalnih i opaque2 inbred linija kukuruza",
volume = "41",
number = "1",
pages = "81-93",
doi = "10.2298/GENSR0901081I",
url = "conv_430"
}
Ignjatović-Micić, D., Marković, K., Ristić, D., Mladenović-Drinić, S., Stanković, S., Lazić-Jančić, V.,& Denić, M.. (2009). Variability analysis of normal and opaque2 maize inbred lines. in Genetika
Društvo genetičara Srbije, Beograd., 41(1), 81-93.
https://doi.org/10.2298/GENSR0901081I
conv_430
Ignjatović-Micić D, Marković K, Ristić D, Mladenović-Drinić S, Stanković S, Lazić-Jančić V, Denić M. Variability analysis of normal and opaque2 maize inbred lines. in Genetika. 2009;41(1):81-93.
doi:10.2298/GENSR0901081I
conv_430 .
Ignjatović-Micić, Dragana, Marković, Ksenija, Ristić, Danijela, Mladenović-Drinić, Snežana, Stanković, Slavica, Lazić-Jančić, Vesna, Denić, Miloje, "Variability analysis of normal and opaque2 maize inbred lines" in Genetika, 41, no. 1 (2009):81-93,
https://doi.org/10.2298/GENSR0901081I .,
conv_430 .
6
7

Quality protein maize: QPM

Ignjatović-Micić, Dragana; Stanković, Goran; Marković, Ksenija; Lazić-Jančić, Vesna; Denić, Miloje

(Društvo genetičara Srbije, Beograd, 2008)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Goran
AU  - Marković, Ksenija
AU  - Lazić-Jančić, Vesna
AU  - Denić, Miloje
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/215
AB  - Quality protein maize (QPM) contains the opaque-2 gene along with numerous modifiers for kernel hardness. Therefore, QPM is maize with high nutritive value of endosperm protein, with substantially higher content of two essential amino acids - lysine and tryptophan, and with good agronomical performances. Although QPM was developed primarily for utilization in the regions where, because of poverty, maize is the main staple food, it has many advantages for production and consumption in other parts of the world, too. QPM can be used for production of conventional and new animal feed, as well as for human nurture. As the rate of animal weight gain is doubled with QPM and portion viability is better, a part of normal maize production could be available for other purposes, such as, for example, ethanol production. Thus, breeding QPM is set as a challenge to produce high quality protein maize with high yield and other important agronomical traits, especially with today's food and feed demands and significance of energy crisis.
AB  - Kukuruz visokog kvaliteta proteina (QPM - Quality Protein Maize) sadrži opaque-2 gen, kao i mnogobrojne modifikatore za tvrdoću zrna. QPM se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma, odnosno značajno većim sadržajem dve esencijalne aminokiseline - lizinom i triptofanom, i istovremeno dobrim agronomskim perfomansama. Mada je QPM stvoren prvenstveno za korišćenje u regionima u kojima je, zbog siromaštva, kukuruz glavna hrana, postoje mnoge prednosti za proizvodnju i korišćenje ovog kukuruza i u ostalim regionima sveta. QPM se može koristiti za proizvodnju konvencionalne i nove hrane za životinje, kao i za ishranu ljudi. Zbog dvostruko bržeg prirasta telesne težine životinja i boljeg iskorišćavanja pripremljenog obroka QPM kukuruza, deo proizvedenog standardnog kukuruza bi se mogao preusmeriti za druge potrebe, kao na primer za proizvodnju etanola. Selekcija kukuruza za poboljšanje kvaliteta proteina, zajedno sa visokim prinosom i dobrim perfomansama drugih značjnih agronomskih svojstava, predstavlja izazov za selekcionere, pogotovo imajući u vidu današnje potrebe za hranom, kao i značaj energetske krize.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Quality protein maize: QPM
T1  - Kukuruz visokog kvaliteta proteina
VL  - 40
IS  - 3
SP  - 205
EP  - 214
DO  - 10.2298/GENSR0803205I
UR  - conv_419
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Stanković, Goran and Marković, Ksenija and Lazić-Jančić, Vesna and Denić, Miloje",
year = "2008",
abstract = "Quality protein maize (QPM) contains the opaque-2 gene along with numerous modifiers for kernel hardness. Therefore, QPM is maize with high nutritive value of endosperm protein, with substantially higher content of two essential amino acids - lysine and tryptophan, and with good agronomical performances. Although QPM was developed primarily for utilization in the regions where, because of poverty, maize is the main staple food, it has many advantages for production and consumption in other parts of the world, too. QPM can be used for production of conventional and new animal feed, as well as for human nurture. As the rate of animal weight gain is doubled with QPM and portion viability is better, a part of normal maize production could be available for other purposes, such as, for example, ethanol production. Thus, breeding QPM is set as a challenge to produce high quality protein maize with high yield and other important agronomical traits, especially with today's food and feed demands and significance of energy crisis., Kukuruz visokog kvaliteta proteina (QPM - Quality Protein Maize) sadrži opaque-2 gen, kao i mnogobrojne modifikatore za tvrdoću zrna. QPM se može definisati kao kukuruz sa visokom nutritivnom vrednošću proteina endosperma, odnosno značajno većim sadržajem dve esencijalne aminokiseline - lizinom i triptofanom, i istovremeno dobrim agronomskim perfomansama. Mada je QPM stvoren prvenstveno za korišćenje u regionima u kojima je, zbog siromaštva, kukuruz glavna hrana, postoje mnoge prednosti za proizvodnju i korišćenje ovog kukuruza i u ostalim regionima sveta. QPM se može koristiti za proizvodnju konvencionalne i nove hrane za životinje, kao i za ishranu ljudi. Zbog dvostruko bržeg prirasta telesne težine životinja i boljeg iskorišćavanja pripremljenog obroka QPM kukuruza, deo proizvedenog standardnog kukuruza bi se mogao preusmeriti za druge potrebe, kao na primer za proizvodnju etanola. Selekcija kukuruza za poboljšanje kvaliteta proteina, zajedno sa visokim prinosom i dobrim perfomansama drugih značjnih agronomskih svojstava, predstavlja izazov za selekcionere, pogotovo imajući u vidu današnje potrebe za hranom, kao i značaj energetske krize.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Quality protein maize: QPM, Kukuruz visokog kvaliteta proteina",
volume = "40",
number = "3",
pages = "205-214",
doi = "10.2298/GENSR0803205I",
url = "conv_419"
}
Ignjatović-Micić, D., Stanković, G., Marković, K., Lazić-Jančić, V.,& Denić, M.. (2008). Quality protein maize: QPM. in Genetika
Društvo genetičara Srbije, Beograd., 40(3), 205-214.
https://doi.org/10.2298/GENSR0803205I
conv_419
Ignjatović-Micić D, Stanković G, Marković K, Lazić-Jančić V, Denić M. Quality protein maize: QPM. in Genetika. 2008;40(3):205-214.
doi:10.2298/GENSR0803205I
conv_419 .
Ignjatović-Micić, Dragana, Stanković, Goran, Marković, Ksenija, Lazić-Jančić, Vesna, Denić, Miloje, "Quality protein maize: QPM" in Genetika, 40, no. 3 (2008):205-214,
https://doi.org/10.2298/GENSR0803205I .,
conv_419 .
4

S1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes

Marković, Ksenija; Nikolić, Ana; Ignjatović-Micić, Dragana; Anđelković, Violeta; Lazić-Jančić, Vesna

(Društvo genetičara Srbije, Beograd, 2008)

TY  - JOUR
AU  - Marković, Ksenija
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Anđelković, Violeta
AU  - Lazić-Jančić, Vesna
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/212
AB  - As water resources for agronomic use become more limiting development of drought tolerant genotypes become increasingly more important. In maize, under drought stress conditions, an increase in the length of anthesis- silking interval and a decrease of grain yield is observed. Different strategies have been proposed to overcome negative effects of drought on maize. Breeders at Maize Research Institute (MRI) have created and used adapted drought tolerant population - DTP-A (DTP79xDTP12xDwarf), that was shown to be a good source of drought tolerance for locally adapted germplasm. In this research s1 families from DTP-A x B84 were scored for anthesis-silking interval and genotyped with SSR markers using BSA approach, with the aim to create a new gene pool for breeding drought tolerant hybrids. SSR analysis was performed in order to identify putative genomic segments and allele pattern differences responsible for expression of ASI, thus providing an insight into the genetic structure of s1 families. Four SSR loci, different in allelic structure between short and long ASI families, were identified. The main characteristic of these differences was the presence or absence of DTP79/DTP12 alleles. It seems that combination of several different genes and alleles influence ASI expression, with DTP79 alleles being beneficial and DTP12 alleles detrimental. Two chromosome regions identified in this work were congruent with qASI regions reported in Maize Genetics and Genomics Database. Clustering revealed that all sASI families grouped with DTP79, but one of the long ASI grouped with these genotypes too. This could indicate that, although total participation of DTP79 genome was similar in all the analyzed families, only a small portion of its genome influence anthesis-silking interval.
AB  - U uslovima stresa suše kod kukuruza dolazi do povećanja dužine intervala između svilanja i metličanja (ASI) i do smanjnja prinosa zrna. U oplemenjivanju se primenjuju različite strategije da bi se prevazišli negativni efekti suše. U Institutu za kukuruz stvorena je DTP-A (DTP79xDTP12xDwarf) populacija, koja se pokazala kao dobar izvor tolerantnosti na sušu za germplazmu adaptiranu na uslove umerenog klimatskog pojasa. U ovim istraživanjima s1 familije DTP-AxB84 su ocenjene za ASI i analizirane SSR markerima primenom analize grupnih uzoraka, radi stvaranja novog genetičkog pula za dobijanje hibrida tolerantnih na sušu. SSR analiza je omogućila identifikaciju potencijalnih genomskih segmanata i identifikaciju razlika u alelnoj strukturi, odgovornih za ekspresiju ASI. Identifikovana su četiri SSR lokusa, koja se razlikuju u alelnoj strukturi familija sa kratkim i dugačkim ASI. Glavna karakteristika ovih razlika je u prisustvu/odsustvu DTP79/DTP12 alela. Najverovatnije da kombinacija nekoliko različitih gena i alela utiče na ekspresiju ASI, pri čemu DTP79 aleli pokazuju pozitivan, a DTP12 negativan efekat. Dva hromozomska regiona koja su identifikovana u ovom radu su prijavljena za ASI i u Maize Genetics and Genomics Database. Rezultati dobijeni klaster analizom su pokazali da sve familije sa kratkim ASI grupišu sa DTP79, mada se i jedna dugačka ASI familija grupiše sa navedenim genotipovima. Ovo može ukazivati da, iako je ukupno učešće DTP79 genoma slično kod svih analiziranih familija, samo mali deo njegovog genoma utiče na ekspresiju ASI.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - S1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes
T1  - S1 familije kao izvor poželjnih alela za oplemenjivanje kukuruza na sušu
VL  - 40
IS  - 1
SP  - 83
EP  - 93
DO  - 10.2298/GENSR0801083M
UR  - conv_412
ER  - 
@article{
author = "Marković, Ksenija and Nikolić, Ana and Ignjatović-Micić, Dragana and Anđelković, Violeta and Lazić-Jančić, Vesna",
year = "2008",
abstract = "As water resources for agronomic use become more limiting development of drought tolerant genotypes become increasingly more important. In maize, under drought stress conditions, an increase in the length of anthesis- silking interval and a decrease of grain yield is observed. Different strategies have been proposed to overcome negative effects of drought on maize. Breeders at Maize Research Institute (MRI) have created and used adapted drought tolerant population - DTP-A (DTP79xDTP12xDwarf), that was shown to be a good source of drought tolerance for locally adapted germplasm. In this research s1 families from DTP-A x B84 were scored for anthesis-silking interval and genotyped with SSR markers using BSA approach, with the aim to create a new gene pool for breeding drought tolerant hybrids. SSR analysis was performed in order to identify putative genomic segments and allele pattern differences responsible for expression of ASI, thus providing an insight into the genetic structure of s1 families. Four SSR loci, different in allelic structure between short and long ASI families, were identified. The main characteristic of these differences was the presence or absence of DTP79/DTP12 alleles. It seems that combination of several different genes and alleles influence ASI expression, with DTP79 alleles being beneficial and DTP12 alleles detrimental. Two chromosome regions identified in this work were congruent with qASI regions reported in Maize Genetics and Genomics Database. Clustering revealed that all sASI families grouped with DTP79, but one of the long ASI grouped with these genotypes too. This could indicate that, although total participation of DTP79 genome was similar in all the analyzed families, only a small portion of its genome influence anthesis-silking interval., U uslovima stresa suše kod kukuruza dolazi do povećanja dužine intervala između svilanja i metličanja (ASI) i do smanjnja prinosa zrna. U oplemenjivanju se primenjuju različite strategije da bi se prevazišli negativni efekti suše. U Institutu za kukuruz stvorena je DTP-A (DTP79xDTP12xDwarf) populacija, koja se pokazala kao dobar izvor tolerantnosti na sušu za germplazmu adaptiranu na uslove umerenog klimatskog pojasa. U ovim istraživanjima s1 familije DTP-AxB84 su ocenjene za ASI i analizirane SSR markerima primenom analize grupnih uzoraka, radi stvaranja novog genetičkog pula za dobijanje hibrida tolerantnih na sušu. SSR analiza je omogućila identifikaciju potencijalnih genomskih segmanata i identifikaciju razlika u alelnoj strukturi, odgovornih za ekspresiju ASI. Identifikovana su četiri SSR lokusa, koja se razlikuju u alelnoj strukturi familija sa kratkim i dugačkim ASI. Glavna karakteristika ovih razlika je u prisustvu/odsustvu DTP79/DTP12 alela. Najverovatnije da kombinacija nekoliko različitih gena i alela utiče na ekspresiju ASI, pri čemu DTP79 aleli pokazuju pozitivan, a DTP12 negativan efekat. Dva hromozomska regiona koja su identifikovana u ovom radu su prijavljena za ASI i u Maize Genetics and Genomics Database. Rezultati dobijeni klaster analizom su pokazali da sve familije sa kratkim ASI grupišu sa DTP79, mada se i jedna dugačka ASI familija grupiše sa navedenim genotipovima. Ovo može ukazivati da, iako je ukupno učešće DTP79 genoma slično kod svih analiziranih familija, samo mali deo njegovog genoma utiče na ekspresiju ASI.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "S1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes, S1 familije kao izvor poželjnih alela za oplemenjivanje kukuruza na sušu",
volume = "40",
number = "1",
pages = "83-93",
doi = "10.2298/GENSR0801083M",
url = "conv_412"
}
Marković, K., Nikolić, A., Ignjatović-Micić, D., Anđelković, V.,& Lazić-Jančić, V.. (2008). S1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes. in Genetika
Društvo genetičara Srbije, Beograd., 40(1), 83-93.
https://doi.org/10.2298/GENSR0801083M
conv_412
Marković K, Nikolić A, Ignjatović-Micić D, Anđelković V, Lazić-Jančić V. S1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes. in Genetika. 2008;40(1):83-93.
doi:10.2298/GENSR0801083M
conv_412 .
Marković, Ksenija, Nikolić, Ana, Ignjatović-Micić, Dragana, Anđelković, Violeta, Lazić-Jančić, Vesna, "S1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes" in Genetika, 40, no. 1 (2008):83-93,
https://doi.org/10.2298/GENSR0801083M .,
conv_412 .
2

SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories

Ignjatović-Micić, Dragana; Mladenović-Drinić, Snežana; Nikolić, Ana; Lazić-Jančić, Vesna

(Maik Nauka/Interperiodica/Springer, New York, 2008)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Mladenović-Drinić, Snežana
AU  - Nikolić, Ana
AU  - Lazić-Jančić, Vesna
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/237
AB  - A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work, 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively, and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Russian Journal of Genetics
T1  - SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories
VL  - 44
IS  - 11
SP  - 1317
EP  - 1324
DO  - 10.1134/S1022795408110100
UR  - conv_718
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Mladenović-Drinić, Snežana and Nikolić, Ana and Lazić-Jančić, Vesna",
year = "2008",
abstract = "A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work, 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively, and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Russian Journal of Genetics",
title = "SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories",
volume = "44",
number = "11",
pages = "1317-1324",
doi = "10.1134/S1022795408110100",
url = "conv_718"
}
Ignjatović-Micić, D., Mladenović-Drinić, S., Nikolić, A.,& Lazić-Jančić, V.. (2008). SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories. in Russian Journal of Genetics
Maik Nauka/Interperiodica/Springer, New York., 44(11), 1317-1324.
https://doi.org/10.1134/S1022795408110100
conv_718
Ignjatović-Micić D, Mladenović-Drinić S, Nikolić A, Lazić-Jančić V. SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories. in Russian Journal of Genetics. 2008;44(11):1317-1324.
doi:10.1134/S1022795408110100
conv_718 .
Ignjatović-Micić, Dragana, Mladenović-Drinić, Snežana, Nikolić, Ana, Lazić-Jančić, Vesna, "SSR Analysis for Genetic Structure and Diversity Determination of Maize Local Populations from Former Yugoslavia Territories" in Russian Journal of Genetics, 44, no. 11 (2008):1317-1324,
https://doi.org/10.1134/S1022795408110100 .,
conv_718 .
2
9
7

Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.

Ignjatović-Micić, Dragana; Mladenović-Drinić, Snežana; Nikolić, Ana; Lazić-Jančić, Vesna

(Društvo genetičara Srbije, Beograd, 2008)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Mladenović-Drinić, Snežana
AU  - Nikolić, Ana
AU  - Lazić-Jančić, Vesna
PY  - 2008
UR  - http://rik.mrizp.rs/handle/123456789/245
AB  - A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers' Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.
VL  - 44
IS  - 11
SP  - 1517
EP  - 1524
UR  - conv_1052
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Mladenović-Drinić, Snežana and Nikolić, Ana and Lazić-Jančić, Vesna",
year = "2008",
abstract = "A collection of 2178 local populations from ex-Yugoslavia territories is maintained in Maize Research Institute (MRI) gene bank. These populations were characterized mainly by morphological markers. In this work 21 local populations belonging to seven different agro-ecological groups have been subjected to SSR analysis using a DNA-pooling strategy. The objective of this work was to develop genetic fingerprints for characterization, identification and classification of the populations, as well as for estimation of their genetic diversity. Also, a DNA-pooling strategy was employed with the aim to certify if it could be applied for population analysis with SSR markers. Statistical analysis of 25 informative SSR primers revealing 224 alleles (bands) showed that the average within-population mean number of alleles was 2.55, the average values for total and within-population diversity were 0.784 and 0.502, respectively and G(ST) value was 0.360. Genetic distance values calculated using Modified Rogers' Distance were in the range from 0.35 to 0.60. The silver staining method of DNA used for bulked samples showed some weakness that could be overcome with a more sensitive staining method. Nevertheless, the results in this work indicate that the SSR analysis of bulks could be used for characterizing a large number of populations in gene banks.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.",
volume = "44",
number = "11",
pages = "1517-1524",
url = "conv_1052"
}
Ignjatović-Micić, D., Mladenović-Drinić, S., Nikolić, A.,& Lazić-Jančić, V.. (2008). Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.. in Genetika
Društvo genetičara Srbije, Beograd., 44(11), 1517-1524.
conv_1052
Ignjatović-Micić D, Mladenović-Drinić S, Nikolić A, Lazić-Jančić V. Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories.. in Genetika. 2008;44(11):1517-1524.
conv_1052 .
Ignjatović-Micić, Dragana, Mladenović-Drinić, Snežana, Nikolić, Ana, Lazić-Jančić, Vesna, "Ssr analysis for genetic structure and diversity determination of maize local populations from former Yugoslavia territories." in Genetika, 44, no. 11 (2008):1517-1524,
conv_1052 .
1

Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations

Marković, Ksenija; Ignjatović-Micić, Dragana; Saratlić, Goran; Lazić-Jančić, Vesna

(Društvo genetičara Srbije, Beograd, 2007)

TY  - JOUR
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Saratlić, Goran
AU  - Lazić-Jančić, Vesna
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/151
AB  - Chromosome regions determining kernel high oil content were identified by RFLP analysis of individual plants from C0 and C9 selection cycles of two maize synthetic populations -DS7u and YuSSSu. Identification of chromosome regions was done with informative RFLP markers (that were identified with BSA earlier) using a single plant approach - analysis of individual plants. This analysis revealed the number of plants carrying alleles that endured frequency alterations during selection process. Statistical analysis (χ2 test) revealed chromosome regions that comprise putative QTLs affecting expression of kernel high oil content of analyzed maize populations. Four regions on chromosomes 1, 6, 7 and 8 were identified in both DS7u and YuSSSu populations. Additional four regions on chromosome 4, 9 and 10 were detected only in DS7u population.
AB  - Hromozomski regioni uključeni u ekspresiju visokog sadržaja ulja u zrnu kukuruza su identifikovani RFLP analizom individualnih biljaka C0 i C9 ciklusa selekcije dve sintetičke populacije kukuruza -DS7u i YuSSSu. Identifikacija hromozomskih regiona je rađena analizom pojedinačnih biljaka iz grupnih uzoraka sa informativnim RFLP markerima (koji su otkriveni analizom grupnih uzoraka u prvom delu eksperimenta). Rezultati ove analize su ukazali na broj biljaka nosioca alela kod kojih je došlo do promena u frekvenciji tokom selekcije. Statističkom analizom (χ2 test) rezultata identifikovani su hromozomski regioni nosioci potencijalnih QTL koji utiču na ekspresiju visokog sadržaja ulja u zrnu analiziranih populacija. Četiri zajednička regiona je identifikovano u obe analizirane populacije, na hromozomima 1, 6, 7 i 8. U populaciji DS7u je identifikovano još četiri regiona, na hromozomima 4, 9 i 10.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations
T1  - Identifikacija hromozomskih regiona uključenih u kontrolu visokog sadržaja ulja u zrnu sintetičkih populacija kukuruza (Zea mays L.)
VL  - 39
IS  - 2
SP  - 197
EP  - 206
DO  - 10.2298/GENSR0702197M
UR  - conv_405
ER  - 
@article{
author = "Marković, Ksenija and Ignjatović-Micić, Dragana and Saratlić, Goran and Lazić-Jančić, Vesna",
year = "2007",
abstract = "Chromosome regions determining kernel high oil content were identified by RFLP analysis of individual plants from C0 and C9 selection cycles of two maize synthetic populations -DS7u and YuSSSu. Identification of chromosome regions was done with informative RFLP markers (that were identified with BSA earlier) using a single plant approach - analysis of individual plants. This analysis revealed the number of plants carrying alleles that endured frequency alterations during selection process. Statistical analysis (χ2 test) revealed chromosome regions that comprise putative QTLs affecting expression of kernel high oil content of analyzed maize populations. Four regions on chromosomes 1, 6, 7 and 8 were identified in both DS7u and YuSSSu populations. Additional four regions on chromosome 4, 9 and 10 were detected only in DS7u population., Hromozomski regioni uključeni u ekspresiju visokog sadržaja ulja u zrnu kukuruza su identifikovani RFLP analizom individualnih biljaka C0 i C9 ciklusa selekcije dve sintetičke populacije kukuruza -DS7u i YuSSSu. Identifikacija hromozomskih regiona je rađena analizom pojedinačnih biljaka iz grupnih uzoraka sa informativnim RFLP markerima (koji su otkriveni analizom grupnih uzoraka u prvom delu eksperimenta). Rezultati ove analize su ukazali na broj biljaka nosioca alela kod kojih je došlo do promena u frekvenciji tokom selekcije. Statističkom analizom (χ2 test) rezultata identifikovani su hromozomski regioni nosioci potencijalnih QTL koji utiču na ekspresiju visokog sadržaja ulja u zrnu analiziranih populacija. Četiri zajednička regiona je identifikovano u obe analizirane populacije, na hromozomima 1, 6, 7 i 8. U populaciji DS7u je identifikovano još četiri regiona, na hromozomima 4, 9 i 10.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations, Identifikacija hromozomskih regiona uključenih u kontrolu visokog sadržaja ulja u zrnu sintetičkih populacija kukuruza (Zea mays L.)",
volume = "39",
number = "2",
pages = "197-206",
doi = "10.2298/GENSR0702197M",
url = "conv_405"
}
Marković, K., Ignjatović-Micić, D., Saratlić, G.,& Lazić-Jančić, V.. (2007). Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations. in Genetika
Društvo genetičara Srbije, Beograd., 39(2), 197-206.
https://doi.org/10.2298/GENSR0702197M
conv_405
Marković K, Ignjatović-Micić D, Saratlić G, Lazić-Jančić V. Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations. in Genetika. 2007;39(2):197-206.
doi:10.2298/GENSR0702197M
conv_405 .
Marković, Ksenija, Ignjatović-Micić, Dragana, Saratlić, Goran, Lazić-Jančić, Vesna, "Identification of chromosome regions determining kernel high oil content in maize (Zea mays L.) Synthetic populations" in Genetika, 39, no. 2 (2007):197-206,
https://doi.org/10.2298/GENSR0702197M .,
conv_405 .
2

The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations

Marković, Ksenija; Ignjatović-Micić, Dragana; Saratlić, Goran; Lazić-Jančić, Vesna

(Savez poljoprivrednih inženjera i tehničara, Beograd, 2007)

TY  - JOUR
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Saratlić, Goran
AU  - Lazić-Jančić, Vesna
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/168
AB  - RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YuSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YuSSSu populations. Additional four RFLPs were identified in DS7u population.
AB  - RFLP lokusi, potencijalni indikatori povećanog sadržaja ulja u zrnu, identifikovani su analizom grupnih uzoraka (Bulk Segregant Analysis - BSA) C0 i C9 ciklusa selekcije dve domaće sintetičke populacije kukuruza - DS7u i YuSSSu. Sadržaj ulja u zrnu biljaka ispitivanih ciklusa selekcije obe populacije utvrđen je NMR spektroskopijom. Analiza genoma grupnih uzoraka početnog (C0) i krajnjeg (C9) ciklusa selekcije DS7u i YuSSSu urađena je pomoću 57 RFLP markera UMC core set. Na osnovu promena u frekvenciji alela u grupnim uzorcima u toku procesa selekcije analiziranih populacija utvrđene su informativne probe - probe potencijalno blisko vezane za QTL uključene u kontrolu povećanog sadržaja ulja u zrnu kukuruza. Identifikovana su četiri zajednička RFLP lokusa u obe analizirane populacije, s tim što je u populaciji DS7u identifikovano još četiri RFLP lokusa potencijalnih indikatora povećanog sadržaja ulja u zrnu kukuruza.
PB  - Savez poljoprivrednih inženjera i tehničara, Beograd
T2  - Journal of Scientific Agricultural Research
T1  - The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations
T1  - Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.)
VL  - 68
IS  - 2
SP  - 5
EP  - 15
UR  - conv_204
ER  - 
@article{
author = "Marković, Ksenija and Ignjatović-Micić, Dragana and Saratlić, Goran and Lazić-Jančić, Vesna",
year = "2007",
abstract = "RFLP loci, potential indicators of kernel high oil content, were identified using bulk segregant analysis (BSA) approach, i.e. bulked samples derived from C0 and C9 selection cycles of two maize synthetic populations - DS7u and YuSSSu. The kernel oil content was determined by the NMR spectroscopy. The genome analysis of bulked samples was carried out with 57 RFLP markers (UMC core set). On the basis of allele frequency differences between cycles of selection, informative RFLP markers (potentially closely linked to QTLs regulating high oil content in maize) were identified. Four common RFLP loci were identified in both DS7u and YuSSSu populations. Additional four RFLPs were identified in DS7u population., RFLP lokusi, potencijalni indikatori povećanog sadržaja ulja u zrnu, identifikovani su analizom grupnih uzoraka (Bulk Segregant Analysis - BSA) C0 i C9 ciklusa selekcije dve domaće sintetičke populacije kukuruza - DS7u i YuSSSu. Sadržaj ulja u zrnu biljaka ispitivanih ciklusa selekcije obe populacije utvrđen je NMR spektroskopijom. Analiza genoma grupnih uzoraka početnog (C0) i krajnjeg (C9) ciklusa selekcije DS7u i YuSSSu urađena je pomoću 57 RFLP markera UMC core set. Na osnovu promena u frekvenciji alela u grupnim uzorcima u toku procesa selekcije analiziranih populacija utvrđene su informativne probe - probe potencijalno blisko vezane za QTL uključene u kontrolu povećanog sadržaja ulja u zrnu kukuruza. Identifikovana su četiri zajednička RFLP lokusa u obe analizirane populacije, s tim što je u populaciji DS7u identifikovano još četiri RFLP lokusa potencijalnih indikatora povećanog sadržaja ulja u zrnu kukuruza.",
publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd",
journal = "Journal of Scientific Agricultural Research",
title = "The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations, Primena molekularnih markera u analizi sadržaja ulja u zrnu grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L.)",
volume = "68",
number = "2",
pages = "5-15",
url = "conv_204"
}
Marković, K., Ignjatović-Micić, D., Saratlić, G.,& Lazić-Jančić, V.. (2007). The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations. in Journal of Scientific Agricultural Research
Savez poljoprivrednih inženjera i tehničara, Beograd., 68(2), 5-15.
conv_204
Marković K, Ignjatović-Micić D, Saratlić G, Lazić-Jančić V. The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations. in Journal of Scientific Agricultural Research. 2007;68(2):5-15.
conv_204 .
Marković, Ksenija, Ignjatović-Micić, Dragana, Saratlić, Goran, Lazić-Jančić, Vesna, "The application of molecular markers in the bulk segregant analysis of kernel oil content in two maize (Zea mays L.) synthetic populations" in Journal of Scientific Agricultural Research, 68, no. 2 (2007):5-15,
conv_204 .

Comparison of AFLP and SSR markers for genetic diversity studies in maize populations

Ignjatović-Micić, Dragana; Mladenović-Drinić, Snežana; Nikolić, Ana; Lazić-Jančić, Vesna

(Maydica-Ist Sper Cerealicoltur, Bergamo, 2007)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Mladenović-Drinić, Snežana
AU  - Nikolić, Ana
AU  - Lazić-Jančić, Vesna
PY  - 2007
UR  - http://rik.mrizp.rs/handle/123456789/175
AB  - Using a set of 18 maize populations belonging to six different agro-ecological groups we report on a comparison of AFLP and SSR techniques to evaluate their applicability for the study of genetic diversity. Also, a DNA-pooling strategy (bulked samples) that proved to be effective in RFLP analysis of maize populations was employed in order to certify if it could be applied with the same success with SSR and AFLP markers. Twenty-five SSR markers generated a total of 224 polymorphic bands, while three AFLP primer combinations generated 188 bands with 79.2% being polymorphic. The average polymorphism content (PIC) was higher for SSRs, i.e. 0.79 for SSRs versus 0.28 for AFLPs. Higher genetic similarity (GS) values were estimated for AFLPs. The Pearson's correlation coefficient calculated for SSR and AFLP genetic similarities was low (0.149) indicating a poor agreement between the two marker systems. The ranking of the agroecological groups according to their mean GS values (the average GS calculated for the three populations representing each group) was congruent for both marker systems and the Pearson's correlation coefficient calculated using these values was 0.853. The cluster analysis did not group populations precisely with expectations based upon their agro-ecological groups, although a much better agreement was accomplished with AFLP analysis. Based on the results in this work it could be concluded that, when using a DNA-pooling strategy, AFLPs are more reliable in assessing genetic diversity of maize populations.
PB  - Maydica-Ist Sper Cerealicoltur, Bergamo
T2  - Maydica
T1  - Comparison of AFLP and SSR markers for genetic diversity studies in maize populations
VL  - 52
IS  - 4
SP  - 399
EP  - 406
UR  - conv_701
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Mladenović-Drinić, Snežana and Nikolić, Ana and Lazić-Jančić, Vesna",
year = "2007",
abstract = "Using a set of 18 maize populations belonging to six different agro-ecological groups we report on a comparison of AFLP and SSR techniques to evaluate their applicability for the study of genetic diversity. Also, a DNA-pooling strategy (bulked samples) that proved to be effective in RFLP analysis of maize populations was employed in order to certify if it could be applied with the same success with SSR and AFLP markers. Twenty-five SSR markers generated a total of 224 polymorphic bands, while three AFLP primer combinations generated 188 bands with 79.2% being polymorphic. The average polymorphism content (PIC) was higher for SSRs, i.e. 0.79 for SSRs versus 0.28 for AFLPs. Higher genetic similarity (GS) values were estimated for AFLPs. The Pearson's correlation coefficient calculated for SSR and AFLP genetic similarities was low (0.149) indicating a poor agreement between the two marker systems. The ranking of the agroecological groups according to their mean GS values (the average GS calculated for the three populations representing each group) was congruent for both marker systems and the Pearson's correlation coefficient calculated using these values was 0.853. The cluster analysis did not group populations precisely with expectations based upon their agro-ecological groups, although a much better agreement was accomplished with AFLP analysis. Based on the results in this work it could be concluded that, when using a DNA-pooling strategy, AFLPs are more reliable in assessing genetic diversity of maize populations.",
publisher = "Maydica-Ist Sper Cerealicoltur, Bergamo",
journal = "Maydica",
title = "Comparison of AFLP and SSR markers for genetic diversity studies in maize populations",
volume = "52",
number = "4",
pages = "399-406",
url = "conv_701"
}
Ignjatović-Micić, D., Mladenović-Drinić, S., Nikolić, A.,& Lazić-Jančić, V.. (2007). Comparison of AFLP and SSR markers for genetic diversity studies in maize populations. in Maydica
Maydica-Ist Sper Cerealicoltur, Bergamo., 52(4), 399-406.
conv_701
Ignjatović-Micić D, Mladenović-Drinić S, Nikolić A, Lazić-Jančić V. Comparison of AFLP and SSR markers for genetic diversity studies in maize populations. in Maydica. 2007;52(4):399-406.
conv_701 .
Ignjatović-Micić, Dragana, Mladenović-Drinić, Snežana, Nikolić, Ana, Lazić-Jančić, Vesna, "Comparison of AFLP and SSR markers for genetic diversity studies in maize populations" in Maydica, 52, no. 4 (2007):399-406,
conv_701 .
7
6

Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers

Ignjatović-Micić, Dragana; Nikolić, Ana; Mladenović-Drinić, Snežana; Vančetović, Jelena; Lazić-Jančić, Vesna

(Društvo genetičara Srbije, Beograd, 2006)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Mladenović-Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Lazić-Jančić, Vesna
PY  - 2006
UR  - http://rik.mrizp.rs/handle/123456789/139
AB  - Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS.
AB  - Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers
T1  - Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK
VL  - 38
IS  - 3
SP  - 227
EP  - 233
DO  - 10.2298/GENSR0603227I
UR  - conv_401
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Nikolić, Ana and Mladenović-Drinić, Snežana and Vančetović, Jelena and Lazić-Jančić, Vesna",
year = "2006",
abstract = "Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank "Zemun Polje", distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of CMS (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of CMS., Trideset izvora citoplazmatične muške sterilnosti (CMS) u okviru lokalnih populacija iz Banke gena Instituta za kukuruz "Zemun Polje" je testirano na prisustvo odgovarajućeg tipa citoplazme multipleks PCR metodom, korišćenjem specifičnih prajmera za T, C i S citoplazmu. Kombinovanje tri para prajmera u jednoj PCR reakciji, koji odgovaraju himernim regionima mitohondrijalnih DNK sekvenci specifičnih za svaki tip citoplazme, omogućilo je pouzdanu identifikaciju glavnih tipova sterilne citoplazme. Detektovano je dominantno prisustvo S tipa citoplazme. Za izvore sterilnosti kod kojih nije identifikovan tip sterilne citoplazme (odsustvo PCR trake) postoji realna sumnja da se radi o novim, neidentifikovanim tipovima citoplazmatične muške sterilnosti.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers, Identifikacija tipova citoplazmatične sterilnosti kukuruza primenom specifičnih prajmera mtDNK",
volume = "38",
number = "3",
pages = "227-233",
doi = "10.2298/GENSR0603227I",
url = "conv_401"
}
Ignjatović-Micić, D., Nikolić, A., Mladenović-Drinić, S., Vančetović, J.,& Lazić-Jančić, V.. (2006). Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers. in Genetika
Društvo genetičara Srbije, Beograd., 38(3), 227-233.
https://doi.org/10.2298/GENSR0603227I
conv_401
Ignjatović-Micić D, Nikolić A, Mladenović-Drinić S, Vančetović J, Lazić-Jančić V. Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers. in Genetika. 2006;38(3):227-233.
doi:10.2298/GENSR0603227I
conv_401 .
Ignjatović-Micić, Dragana, Nikolić, Ana, Mladenović-Drinić, Snežana, Vančetović, Jelena, Lazić-Jančić, Vesna, "Identification of sterile cytoplasm (CMS) in maize by using specific mtDNA primers" in Genetika, 38, no. 3 (2006):227-233,
https://doi.org/10.2298/GENSR0603227I .,
conv_401 .
1

Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L) synthetic populations

Ignjatović-Micić, Dragana; Marković, Ksenija; Lazić-Jančić, Vesna

(Društvo genetičara Srbije, Beograd, 2006)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Marković, Ksenija
AU  - Lazić-Jančić, Vesna
PY  - 2006
UR  - http://rik.mrizp.rs/handle/123456789/132
AB  - Chromosome regions which carry potential QTLs for high grain yield in two synthetic maize populations - B73xMol7 and LlxMol7, were identified by bulk segregant analysis (BSA). Yield was evaluated on F2 testcross families in field trials using a Nested design. Based on yield data, p3 families with the corresponding highest and lowest testcross yields were selected for BSA. Genome analysis of F3 families was carried out with 58 RFLP markers. Allele frequency differences were detected at four RFLP loci n chromosomes 1, 2, 6 and 10 (B73xMol7), i.e. four RFLP loci on chromosomes 1, 2, 6 and 9 (LlxMo17). Only one region, at chromosome 6, was identified in both populations, but with two different RFLP markers. In B73xMol7 it was umc65 and in LlxMol7 umc2l RFLP marker. Bulk segregant analysis was shown to be a quick and informative method for identification of chromosome regions which determine high yield expression in maize, i.e. for identification of RFLP markers closely linked to potential genes involved in expression of the trait.
AB  - Identifikacija hromozomskih regiona potencijalnih nosioca QTL uključenih u ekspresiju visokog prinosa zrna kukuruza kod dve sintetičke populacije B73xMol7 i LlxMol7, urađena je analizom grupnih uzoraka. Prinos je određen na familijama test-ukrštanja F2 biljaka u poljskim ogledima, po Nested dizajnu. Na osnovu podataka o prinosu odabrane su F3 familije sa najvećim i najmanjim prinosom odgovarajućih familija test-ukrštanja. Analiza genoma visoko i nisko prinosnih grupnih uzoraka F3 familija je urađena pomoću 58 RFLP markera. Na osnovu razlika u frekvenciji alela identifikovana su četiri RFLP lokusa na hromozomima l, 2, 6 i 10 (B73xMol7), odnosno četiri RFLP lokusa na hromozomima l, 2, 6 i 9 (LlxMol7). Samo je jedan region, na hromozomu 6 identifikovan u oba ukrštanja, ali sa dva različita markera - umc65 u B73xMol7 i umc21 u LlxMol7. Analiza grupnih uzoraka se pokazala kao brza i informativna metoda u identifikaciji hromozomskih regiona koji determinišu ispoljavanje visokog prinosa kod kukuruza, tj. u identifikaciji RFLP markera blisko vezanih za potencijalne gene uključene u ekspresiju ove osobine.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L) synthetic populations
T1  - Primena molekularnih markera u analizi prinosa grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L)
VL  - 38
IS  - 1
SP  - 59
EP  - 66
DO  - 10.2298/GENSR0601059I
UR  - conv_399
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Marković, Ksenija and Lazić-Jančić, Vesna",
year = "2006",
abstract = "Chromosome regions which carry potential QTLs for high grain yield in two synthetic maize populations - B73xMol7 and LlxMol7, were identified by bulk segregant analysis (BSA). Yield was evaluated on F2 testcross families in field trials using a Nested design. Based on yield data, p3 families with the corresponding highest and lowest testcross yields were selected for BSA. Genome analysis of F3 families was carried out with 58 RFLP markers. Allele frequency differences were detected at four RFLP loci n chromosomes 1, 2, 6 and 10 (B73xMol7), i.e. four RFLP loci on chromosomes 1, 2, 6 and 9 (LlxMo17). Only one region, at chromosome 6, was identified in both populations, but with two different RFLP markers. In B73xMol7 it was umc65 and in LlxMol7 umc2l RFLP marker. Bulk segregant analysis was shown to be a quick and informative method for identification of chromosome regions which determine high yield expression in maize, i.e. for identification of RFLP markers closely linked to potential genes involved in expression of the trait., Identifikacija hromozomskih regiona potencijalnih nosioca QTL uključenih u ekspresiju visokog prinosa zrna kukuruza kod dve sintetičke populacije B73xMol7 i LlxMol7, urađena je analizom grupnih uzoraka. Prinos je određen na familijama test-ukrštanja F2 biljaka u poljskim ogledima, po Nested dizajnu. Na osnovu podataka o prinosu odabrane su F3 familije sa najvećim i najmanjim prinosom odgovarajućih familija test-ukrštanja. Analiza genoma visoko i nisko prinosnih grupnih uzoraka F3 familija je urađena pomoću 58 RFLP markera. Na osnovu razlika u frekvenciji alela identifikovana su četiri RFLP lokusa na hromozomima l, 2, 6 i 10 (B73xMol7), odnosno četiri RFLP lokusa na hromozomima l, 2, 6 i 9 (LlxMol7). Samo je jedan region, na hromozomu 6 identifikovan u oba ukrštanja, ali sa dva različita markera - umc65 u B73xMol7 i umc21 u LlxMol7. Analiza grupnih uzoraka se pokazala kao brza i informativna metoda u identifikaciji hromozomskih regiona koji determinišu ispoljavanje visokog prinosa kod kukuruza, tj. u identifikaciji RFLP markera blisko vezanih za potencijalne gene uključene u ekspresiju ove osobine.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L) synthetic populations, Primena molekularnih markera u analizi prinosa grupnih uzoraka sintetičkih populacija kukuruza (Zea mays L)",
volume = "38",
number = "1",
pages = "59-66",
doi = "10.2298/GENSR0601059I",
url = "conv_399"
}
Ignjatović-Micić, D., Marković, K.,& Lazić-Jančić, V.. (2006). Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L) synthetic populations. in Genetika
Društvo genetičara Srbije, Beograd., 38(1), 59-66.
https://doi.org/10.2298/GENSR0601059I
conv_399
Ignjatović-Micić D, Marković K, Lazić-Jančić V. Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L) synthetic populations. in Genetika. 2006;38(1):59-66.
doi:10.2298/GENSR0601059I
conv_399 .
Ignjatović-Micić, Dragana, Marković, Ksenija, Lazić-Jančić, Vesna, "Application of molecular markers in bulk segragant analysis of yield in maize (Zea mays L) synthetic populations" in Genetika, 38, no. 1 (2006):59-66,
https://doi.org/10.2298/GENSR0601059I .,
conv_399 .
1

QTL detection for drought tolerance in composite maize populations

Ignjatović-Micić, Dragana; Marković, Ksenija; Lazić-Jančić, Vesna

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2006)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Marković, Ksenija
AU  - Lazić-Jančić, Vesna
PY  - 2006
UR  - http://rik.mrizp.rs/handle/123456789/116
AB  - The most significant QTLs for leaf ABA and root characteristics are shown to be on chromosomes 1 (bin 1.06) and 2 (bin 2.04). Assuming that the same QTLs could be present in different genotypes, the objective of this work was to ascertain if the QTLs with major effect detected i n one genotype could be detected in a different genetic background with different molecular markers of that chromosome region. Also, bulk segregant analysis (BSA) of 10 tolerant and 10 susceptible to drought maize populations was performed in order to confirm the potential of this approach in informative marker identification. Probes umc1601, umc1776, bnlg2248, dupSSR27 and bnlg2246 detected allele frequency differences between tolerant and susceptible populations of over 14%. It was also shown that BSA can identify informative molecular markers, although marker analysis of individual plants is necessary for final conclusion about linkage of the marker and the trait of interest.
AB  - Najznačajniji QTL-ovi za sadržaj ABA u listu i neke karakteristike korenovog sistema se, prema podacima iz literature, nalaze na hromozomima 1 (bin 1.06) i 2 (bin 2.04). Pretpostavljajući da isti QTL-ovi mogu biti prisutni u različitim genotipovima, cilj ovog rada je bio da se utvrdi da li QTL-vi sa major efektom u jednom genotipu mogu biti detektovani u različitim genetičkim okruženjima, koristeći molekularne markers datog hromozomskog regiona. Takođe, analiza grupnih uzoraka 10 tolerantnih i 10 osetljivih populacija na sušu je urađena da bi se proverila ispravnost ovog pristupa u identifikaciji informativnih markera. Probama umc1601, umc1776, bnlg2248, dupSSR27 i bnlg2246 detektovana je razlika u frekvenciji alela između tolerantnih i osetljivih populacija veća od 14%, što ukazuje na moguću povezanost markera i svojstva od interesa, mada je za konačan zaključak neophodno uraditi analizu pojedinačnih biljaka grupnih uzoraka.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - QTL detection for drought tolerance in composite maize populations
T1  - Detekcija QTL-ova za tolerantnost na sušu kompozitnih populacija kukuruza (Zea mays L.)
VL  - 12
IS  - 3-4
SP  - 49
EP  - 53
UR  - conv_264
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Marković, Ksenija and Lazić-Jančić, Vesna",
year = "2006",
abstract = "The most significant QTLs for leaf ABA and root characteristics are shown to be on chromosomes 1 (bin 1.06) and 2 (bin 2.04). Assuming that the same QTLs could be present in different genotypes, the objective of this work was to ascertain if the QTLs with major effect detected i n one genotype could be detected in a different genetic background with different molecular markers of that chromosome region. Also, bulk segregant analysis (BSA) of 10 tolerant and 10 susceptible to drought maize populations was performed in order to confirm the potential of this approach in informative marker identification. Probes umc1601, umc1776, bnlg2248, dupSSR27 and bnlg2246 detected allele frequency differences between tolerant and susceptible populations of over 14%. It was also shown that BSA can identify informative molecular markers, although marker analysis of individual plants is necessary for final conclusion about linkage of the marker and the trait of interest., Najznačajniji QTL-ovi za sadržaj ABA u listu i neke karakteristike korenovog sistema se, prema podacima iz literature, nalaze na hromozomima 1 (bin 1.06) i 2 (bin 2.04). Pretpostavljajući da isti QTL-ovi mogu biti prisutni u različitim genotipovima, cilj ovog rada je bio da se utvrdi da li QTL-vi sa major efektom u jednom genotipu mogu biti detektovani u različitim genetičkim okruženjima, koristeći molekularne markers datog hromozomskog regiona. Takođe, analiza grupnih uzoraka 10 tolerantnih i 10 osetljivih populacija na sušu je urađena da bi se proverila ispravnost ovog pristupa u identifikaciji informativnih markera. Probama umc1601, umc1776, bnlg2248, dupSSR27 i bnlg2246 detektovana je razlika u frekvenciji alela između tolerantnih i osetljivih populacija veća od 14%, što ukazuje na moguću povezanost markera i svojstva od interesa, mada je za konačan zaključak neophodno uraditi analizu pojedinačnih biljaka grupnih uzoraka.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "QTL detection for drought tolerance in composite maize populations, Detekcija QTL-ova za tolerantnost na sušu kompozitnih populacija kukuruza (Zea mays L.)",
volume = "12",
number = "3-4",
pages = "49-53",
url = "conv_264"
}
Ignjatović-Micić, D., Marković, K.,& Lazić-Jančić, V.. (2006). QTL detection for drought tolerance in composite maize populations. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 12(3-4), 49-53.
conv_264
Ignjatović-Micić D, Marković K, Lazić-Jančić V. QTL detection for drought tolerance in composite maize populations. in Selekcija i semenarstvo. 2006;12(3-4):49-53.
conv_264 .
Ignjatović-Micić, Dragana, Marković, Ksenija, Lazić-Jančić, Vesna, "QTL detection for drought tolerance in composite maize populations" in Selekcija i semenarstvo, 12, no. 3-4 (2006):49-53,
conv_264 .

RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions

Ignjatović-Micić, Dragana; Corić, T.; Kovačević, Dragan; Marković, Ksenija; Lazić-Jančić, Vesna

(2003)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Corić, T.
AU  - Kovačević, Dragan
AU  - Marković, Ksenija
AU  - Lazić-Jančić, Vesna
PY  - 2003
UR  - http://rik.mrizp.rs/handle/123456789/52
AB  - The genebank in Maize Research Institute "Zemun Polje" maintains the collection of 2178 local populations of maize, characterised and classified using morphological markers. In this work 13 local populations, some of which were suspected to be duplicate accessions, were subjected to RAPD analysis with OPB 1-20 primers, as well as to RFLP analysis with 30 probes in combination with three restriction enzymes. Data thus obtained were used for calculation of Nei's genetic distances among populations and the resulting distance matrix was used for cluster analysis by UPGMA method. Genetic distances of analysed populations ranged between 0.1311 and 0.5075. Genetic distances calculated from RAPD and RFLP data were highly similar, leading to the conclusion that both markers revealed sufficient amount of genetic polymorphism among populations. Both methods showed a high degree of discrimination between populations, so they can be successfully used for polymorphism validation, genetic distance estimation among maize populations and screening of suspected duplicates in maize gene bank.
T2  - Maydica
T1  - RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions
VL  - 48
IS  - 2
SP  - 153
EP  - 159
UR  - conv_1062
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Corić, T. and Kovačević, Dragan and Marković, Ksenija and Lazić-Jančić, Vesna",
year = "2003",
abstract = "The genebank in Maize Research Institute "Zemun Polje" maintains the collection of 2178 local populations of maize, characterised and classified using morphological markers. In this work 13 local populations, some of which were suspected to be duplicate accessions, were subjected to RAPD analysis with OPB 1-20 primers, as well as to RFLP analysis with 30 probes in combination with three restriction enzymes. Data thus obtained were used for calculation of Nei's genetic distances among populations and the resulting distance matrix was used for cluster analysis by UPGMA method. Genetic distances of analysed populations ranged between 0.1311 and 0.5075. Genetic distances calculated from RAPD and RFLP data were highly similar, leading to the conclusion that both markers revealed sufficient amount of genetic polymorphism among populations. Both methods showed a high degree of discrimination between populations, so they can be successfully used for polymorphism validation, genetic distance estimation among maize populations and screening of suspected duplicates in maize gene bank.",
journal = "Maydica",
title = "RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions",
volume = "48",
number = "2",
pages = "153-159",
url = "conv_1062"
}
Ignjatović-Micić, D., Corić, T., Kovačević, D., Marković, K.,& Lazić-Jančić, V.. (2003). RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions. in Maydica, 48(2), 153-159.
conv_1062
Ignjatović-Micić D, Corić T, Kovačević D, Marković K, Lazić-Jančić V. RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions. in Maydica. 2003;48(2):153-159.
conv_1062 .
Ignjatović-Micić, Dragana, Corić, T., Kovačević, Dragan, Marković, Ksenija, Lazić-Jančić, Vesna, "RFLP and RAPD analysis of maize (Zea mays L.) local populations for identification of variability and duplicate accessions" in Maydica, 48, no. 2 (2003):153-159,
conv_1062 .
5