Ignjatović-Micić, Dragana

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orcid::0000-0003-3584-3887
  • Ignjatović-Micić, Dragana (84)
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Author's Bibliography

Nutritional and cost effects of adapted quality protein maize on broiler feeding

Kostadinović, Marija; Ristić, Danijela; Vančetović, Jelena; Delić, Nenad; Ignjatović-Micić, Dragana

(Belgrade : Maize Research Institute, Zemun Polje, 2022)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Vančetović, Jelena
AU  - Delić, Nenad
AU  - Ignjatović-Micić, Dragana
PY  - 2022
UR  - http://rik.mrizp.rs/handle/123456789/967
AB  - ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.
PB  - Belgrade : Maize Research Institute, Zemun Polje
C3  - 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
T1  - Nutritional and cost effects of adapted quality protein maize on broiler feeding
SP  - 70
ER  - 
@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Vančetović, Jelena and Delić, Nenad and Ignjatović-Micić, Dragana",
year = "2022",
abstract = "ZPQPM13 is a maize hybrid with improved protein quality, adapted to temperate climate growth conditions, developed by crossing a QPM version of ZPL5 commercial line (developed by marker assisted selection) and GS-6 (a QPM line with 50% tropical and 50% temperate germplasm). This hybrid has grain yield as commercial hybrids, over 90% of hard endosperm, contents of tryptophan and protein over 0.075% and 10%, respectively, and stability of tryptophan content over different locations and years. ZPQPM13 was tested for use in broiler feeds, with the aim to reduce utilization of costly synthetic lysine or soybean components. The feeding experiment on Ross 308 broilers was divided into two groups – control (fed with standard maize) and treatment (fed with QPM). In treatment group diets, ZPQPM13 was increased and soybean component decreased for 3%. Chemical analyses revealed higher contents of limiting essential amino acids in mercantile ZPQPM13 compared to standard maize – 0.44 vs. 0.25 % lysine, 0.077 vs. 0.070 % tryptophan and 0.39 vs. 0.27 % threonine. Duration of each feeding trial was 42 days, comprising three phases - starter (1-14 days), grower (15-35 days) and finisher (36-42 days). Feed intake (FI), body mass gain (BMG), average daily gain (ADG) and feed conversion ratio (FCR) were calculated at the end of each phase. In treatment group, FI was reduced, while BMG and ADG were higher in starter phase but lower in grower and finisher phases. FCR was lower in all three phases in treatment group – 1.13 vs. 1.30, 1.25 vs. 1.33 and 1.28 vs. 1.45. Although a small drawback is that final weight was 3.4 % higher in control broilers, comparison of feed and meat prices show that financial benefits of using QPM are still significant compared to using standard maize in feeds, as soybean is four times more expensive than maize.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts",
title = "Nutritional and cost effects of adapted quality protein maize on broiler feeding",
pages = "70"
}
Kostadinović, M., Ristić, D., Vančetović, J., Delić, N.,& Ignjatović-Micić, D.. (2022). Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 70.
Kostadinović M, Ristić D, Vančetović J, Delić N, Ignjatović-Micić D. Nutritional and cost effects of adapted quality protein maize on broiler feeding. in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts. 2022;:70..
Kostadinović, Marija, Ristić, Danijela, Vančetović, Jelena, Delić, Nenad, Ignjatović-Micić, Dragana, "Nutritional and cost effects of adapted quality protein maize on broiler feeding" in 25. EUCARPIA Maize and sorghum conference:"Current challenges and new methods for maize and sorghum breeding", 30.05-02.06.2022., Belgrade - Book of abstracts (2022):70.

Adapted quality protein maize for broiler feeds

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Delić, Nenad

(Belgrade : Maize Research Institute, Zemun Polje, 2021)

TY  - GEN
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Delić, Nenad
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/969
AB  - Maize has low nutritional quality due to poor content of essential amino
acids lysine and tryptophan in the dominant seed storage protein fraction,
zeins. However, Quality Protein Maize (QPM), a variety of opaque2 maize
but with good agronomic traits, can have 60% to 100% higher content of
lysine than standard maize. Monogastric animals (pigs, poultry, fish), like
humans, cannot synthesize lysine and tryptophan de novo and thus these
amino acids must be supplied through diets. QPM was primarily developed
for human consumption to overcome malnutrition in countries where maize
is staple food. Thus, QPM is of tropical origin and its adaptation to temperate
regions is frequently hampered by the retained exotic germplasm.
Development of adapted QPM hybrids is mainly aimed for feed industry. It
has been shown that substituting standard maize with QPM in feed diets
could be profitable due to improved weight gain, feed conversion ratio and
decreasing of dietary lysine supplementation. Maize Research Institute,
Zemun Polje has a program on converting elite inbred lines through marker
assisted breeding into their QPM counterparts and developing commercial
QPM hybrids. Up to now, two inbred lines have been converted, while eight
lines have passed through three or four generations of backcrossing and one
generation of selfing. Tryptophan content in the converted selfed plants was
in the range from 0.080 to 0.093. Besides developing QPM counterparts of
commercial hybrids, one QPM hybrid obtained by crossing a converted elite
inbred line and an adapted tropical inbred line was developed and tested over
different locations and in different years. This hybrid is currently used in
feeding experiments with the objective to test effects of replacing standard
maize with QPM in diets on broiler performances.
PB  - Belgrade : Maize Research Institute, Zemun Polje
T2  - International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts
T1  - Adapted quality protein maize for broiler feeds
SP  - 75
ER  - 
@misc{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Delić, Nenad",
year = "2021",
abstract = "Maize has low nutritional quality due to poor content of essential amino
acids lysine and tryptophan in the dominant seed storage protein fraction,
zeins. However, Quality Protein Maize (QPM), a variety of opaque2 maize
but with good agronomic traits, can have 60% to 100% higher content of
lysine than standard maize. Monogastric animals (pigs, poultry, fish), like
humans, cannot synthesize lysine and tryptophan de novo and thus these
amino acids must be supplied through diets. QPM was primarily developed
for human consumption to overcome malnutrition in countries where maize
is staple food. Thus, QPM is of tropical origin and its adaptation to temperate
regions is frequently hampered by the retained exotic germplasm.
Development of adapted QPM hybrids is mainly aimed for feed industry. It
has been shown that substituting standard maize with QPM in feed diets
could be profitable due to improved weight gain, feed conversion ratio and
decreasing of dietary lysine supplementation. Maize Research Institute,
Zemun Polje has a program on converting elite inbred lines through marker
assisted breeding into their QPM counterparts and developing commercial
QPM hybrids. Up to now, two inbred lines have been converted, while eight
lines have passed through three or four generations of backcrossing and one
generation of selfing. Tryptophan content in the converted selfed plants was
in the range from 0.080 to 0.093. Besides developing QPM counterparts of
commercial hybrids, one QPM hybrid obtained by crossing a converted elite
inbred line and an adapted tropical inbred line was developed and tested over
different locations and in different years. This hybrid is currently used in
feeding experiments with the objective to test effects of replacing standard
maize with QPM in diets on broiler performances.",
publisher = "Belgrade : Maize Research Institute, Zemun Polje",
journal = "International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts",
title = "Adapted quality protein maize for broiler feeds",
pages = "75"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J.,& Delić, N.. (2021). Adapted quality protein maize for broiler feeds. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts
Belgrade : Maize Research Institute, Zemun Polje., 75.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Delić N. Adapted quality protein maize for broiler feeds. in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts. 2021;:75..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Delić, Nenad, "Adapted quality protein maize for broiler feeds" in International conference: "The frontiers of science and technology in crop breeding and production conference", Belgrade, 8-9.06.2021. - Book of abstracts (2021):75.

Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions

Božić, Manja; Stanojević, Aleksandra; Marković, Ksenija; Ignjatović-Micić, Dragana; Nikolić, Dragana; Milivojević, Marija; Nikolić, Ana

(Belgrade : Serbian Genetics Society, 2021)

TY  - JOUR
AU  - Božić, Manja
AU  - Stanojević, Aleksandra
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Dragana
AU  - Milivojević, Marija
AU  - Nikolić, Ana
PY  - 2021
UR  - http://rik.mrizp.rs/handle/123456789/832
AB  - Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance.
AB  - Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.
PB  - Belgrade : Serbian Genetics Society
T2  - Genetika
T1  - Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions
VL  - 53
IS  - 3
SP  - 1147
EP  - 1165
DO  - 10.2298/GENSR2103147B
ER  - 
@article{
author = "Božić, Manja and Stanojević, Aleksandra and Marković, Ksenija and Ignjatović-Micić, Dragana and Nikolić, Dragana and Milivojević, Marija and Nikolić, Ana",
year = "2021",
abstract = "Maize is one of the most important crop species worldwide, but also extremely
susceptible to the effects of increasingly higher temperatures and drought during the
summer and its flowering and grain filling stage. Different strategies are being utilized to
ensure a satisfying yield potential and quality even in the extremely unfavourable
environmental conditions, which are the result of climate change. Some of them are
cropping pattern changes and sowing alterations, including earlier sowing. Since this
implies the exposure to suboptimal temperatures during early developmental stages, it
leads to a demand for developing maize lines tolerant to low temperatures during these
stages. This research focuses on the first phase of germination, imbibition.
Maize tolerance to low temperatures is a complex trait that includes different mechanisms
and strategies that all work together to ensure adaptation and survival, such as cell
membrane changes, antioxidative system activation, etc. Additionally, the low
temperature response of different maize inbreds varies substantially, so recognizing the
different ways in which they respond to low temperatures during imbibition and other
earlier stages of development is crucial. To accomplish this, seeds of two maize inbred
lines of contrasting susceptibility to low temperatures were selected and exposed to
control (20° C) and low temperature (8°C) conditions during the first 24h of imbition andthen further analyzed to assess their response. This included germination and tetrazolium
assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation;
determining the antioxidative capacity by assessing superoxide dismutase (SOD) and
catalase (CAT) activity; and expression analysis of four genes included in the low
temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid
desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2).
The results showed that, while there is not a significant difference in their germination
rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving
the low temperature period. Significant differences between them were found in cell
leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The
present research brings light to our understanding of the effect of low temperatures on the
first germination stage, - imbibition. It highlights the importance of choosing the right
inbreds for earlier sowing and points to certain routes that could be taken for improving
and accelerating the breeding process for low temperature tolerance., Kukuruz je usev od izuzetne važnosti za poljoprivredu, koji se takođe odlikuje značajnom
osetljivošću na visoke temperature i sušu tokom letnjih meseci, odnosno tokom faze cvetanja i
nalivanja zrna. Jedna od strategija obezbeđivanja zadovoljavajuće produktivnosti i prinosa
kukuruza, uprkos nepovoljnim uslovima životne sredine, jeste ranija setva. Ipak u tom slučaju su
biljke kukuruza izložene suboptimalnim temperaturama u ranim fazama razvića, što stvara
potrebu za razvojem linija tolerantnih na niske temperature u tim fazama, što je i fokus ovog
istraživanja. Odgovor kukuruza na niske temperature je kompleksan i uključuje niz različitih
mehanizama i strategija koje obezbeđuju opstanak, a takođe zavisi i od samog genotipa, faze
razvića, itd. Iz tog razloga, odgovor kukuruza na niske temperature (8°C) je posmatran tokom
faze imbibicije, kod dva genotipa, i to na nivou promena genske ekspresije (gid1; fad2; fad6;
pap2), aktivnosti antioksidativnih enzima (SOD, CAT), integriteta ćelijskih membrana, stopa
germinacije, kao i samog preživljavanja. Rezultati ukazuju da postoje statistički značajne razlike
između genotipova kada je reč o stopi preživljavanja, integritetu ćelijskih membrana i ekspresiji
gena gid1i fad6 (p<0,05). Ovo istraživanje ukazuje na moguće mehanizme aklimatizacije na
niske temperature tokom faze imbibicije i ističe značaj odabira odgovarajućih linija za raniju
setvu.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "Genetika",
title = "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions",
volume = "53",
number = "3",
pages = "1147-1165",
doi = "10.2298/GENSR2103147B"
}
Božić, M., Stanojević, A., Marković, K., Ignjatović-Micić, D., Nikolić, D., Milivojević, M.,& Nikolić, A.. (2021). Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika
Belgrade : Serbian Genetics Society., 53(3), 1147-1165.
https://doi.org/10.2298/GENSR2103147B
Božić M, Stanojević A, Marković K, Ignjatović-Micić D, Nikolić D, Milivojević M, Nikolić A. Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions. in Genetika. 2021;53(3):1147-1165.
doi:10.2298/GENSR2103147B .
Božić, Manja, Stanojević, Aleksandra, Marković, Ksenija, Ignjatović-Micić, Dragana, Nikolić, Dragana, Milivojević, Marija, Nikolić, Ana, "Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions" in Genetika, 53, no. 3 (2021):1147-1165,
https://doi.org/10.2298/GENSR2103147B . .

Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation

Kostadinović, Marija; Janjić, Jovana; Ristić, Danijela; Đorđević-Melnik, Olivera; Božinović, Sofija; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2020)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Janjić, Jovana
AU  - Ristić, Danijela
AU  - Đorđević-Melnik, Olivera
AU  - Božinović, Sofija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/962
AB  - This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
T1  - Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation
SP  - 37
EP  - 44
ER  - 
@conference{
author = "Kostadinović, Marija and Janjić, Jovana and Ristić, Danijela and Đorđević-Melnik, Olivera and Božinović, Sofija and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2020",
abstract = "This paper is a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions using the marker assisted selection. When SSR marker specific to the crtRB1 gene has been tested on parental lines, distinct polymorphism was observed between the donor and recurrent parents. The same marker has also been validated in BC1 generation when heterozygous individuals were clearly distinguishable from the homozygous dominants. The results presented herein refer to two-level selection procedure in BC2 generation. First, BC2 plants were analyzed with crtRB1-specific molecular marker to separate heterozygotes from dominant homozygotes. Percentage of heterozygous plants was approximately 50%, which was in accordance with the expected Mendelian ratio of 1:1. Second, the selected heterozygotes were screened with 30 polymorphic SSR markers distributed throughout the maize genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The RPG values among three analyzed parental lines and their respective progenies ranged from 85-99%. For each line separately, RPG values were: 86-97% (RP1), 90-95% (RP2) and 85-99% (RP3). Plants with RPG above 95% were selfed to produce BC2F2 generation in which homozygous recessive individuals would be identified. Those genotypes will be subjected to biochemical and phenotypic evaluation to confirm their nutritional and agronomical superiority. Finally, these β-carotene enriched lines, as well as the resulting improved hybrids, can be used in the biofortification programs.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings",
title = "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation",
pages = "37-44"
}
Kostadinović, M., Janjić, J., Ristić, D., Đorđević-Melnik, O., Božinović, S., Ignjatović-Micić, D.,& Vančetović, J.. (2020). Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 37-44.
Kostadinović M, Janjić J, Ristić D, Đorđević-Melnik O, Božinović S, Ignjatović-Micić D, Vančetović J. Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation. in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings. 2020;:37-44..
Kostadinović, Marija, Janjić, Jovana, Ristić, Danijela, Đorđević-Melnik, Olivera, Božinović, Sofija, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted selection for β-carotene rich maize: two-level selection procedure in BC2 generation" in 11. international scientific agriculture symposium "Agrosym 2020", 08-09.10.2020., Jahorina, B i H - Book of proceedings (2020):37-44.

Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality

Ignjatović-Micić, Dragana; Kostadinović, Marija; Božinović, Sofija; Đorđević-Melnik, Olivera; Stanković, Goran; Delić, Nenad; Vančetović, Jelena

(Chile : Instituto de Investigaciones Agropecuarias, INIA, 2020)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Stanković, Goran
AU  - Delić, Nenad
AU  - Vančetović, Jelena
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/819
AB  - Quality protein maize (QPM) (Zea mays L.) is primarily used for food in countries of tropical and sub-tropical regions
where maize is the main source of protein. Although its cultivation in temperate regions is hampered by residues of
exotic germplasm, it could be beneficial for use in livestock feeds as it was shown that substitution of standard maize
with QPM can improve livestock characteristics and decrease dietary lysine supplementation. The aim of this study
was to test 11 QPM hybrids obtained by crossing adapted QPM inbred lines for their performance in field trials in 2
yr at four locations, as well as to determine their relevant biochemical components. The main reason for rejecting nine
QPM hybrids was low grain yield, standard hybrids had higher yields on average for 37.8%. Hybrid ZPQPM6 had good
agronomic characteristics, but its biochemical components were nonsignificantly different from the standard hybrid.
Only hybrid ZPQPM13 met necessary criteria, grain yield comparable with standard hybrids, high tryptophan content
in different environments (average 0.083%) and hard endosperm (average score 1.87). Lysine content, measured after
mercantile production, was 0.44%. Quality index, although below the QPM threshold (which is 0.80%) was significantly
higher (p < 0.05) in ZPQPM13 in comparison with standard hybrid, indicating improved nutritional quality of the protein.
The results indicated that presence of exotic germplasm in these QPM hybrids is a consequential difficulty and that in their
parental inbred lines at least one more backcross with temperate germplasm should be done to select better adapted QPM.
PB  - Chile : Instituto de Investigaciones Agropecuarias, INIA
T2  - Chilean Journal of Agricultural Research
T1  - Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality
VL  - 80
IS  - 4
SP  - 598
EP  - 607
DO  - 10.4067/S0718-58392020000400598
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Kostadinović, Marija and Božinović, Sofija and Đorđević-Melnik, Olivera and Stanković, Goran and Delić, Nenad and Vančetović, Jelena",
year = "2020",
abstract = "Quality protein maize (QPM) (Zea mays L.) is primarily used for food in countries of tropical and sub-tropical regions
where maize is the main source of protein. Although its cultivation in temperate regions is hampered by residues of
exotic germplasm, it could be beneficial for use in livestock feeds as it was shown that substitution of standard maize
with QPM can improve livestock characteristics and decrease dietary lysine supplementation. The aim of this study
was to test 11 QPM hybrids obtained by crossing adapted QPM inbred lines for their performance in field trials in 2
yr at four locations, as well as to determine their relevant biochemical components. The main reason for rejecting nine
QPM hybrids was low grain yield, standard hybrids had higher yields on average for 37.8%. Hybrid ZPQPM6 had good
agronomic characteristics, but its biochemical components were nonsignificantly different from the standard hybrid.
Only hybrid ZPQPM13 met necessary criteria, grain yield comparable with standard hybrids, high tryptophan content
in different environments (average 0.083%) and hard endosperm (average score 1.87). Lysine content, measured after
mercantile production, was 0.44%. Quality index, although below the QPM threshold (which is 0.80%) was significantly
higher (p < 0.05) in ZPQPM13 in comparison with standard hybrid, indicating improved nutritional quality of the protein.
The results indicated that presence of exotic germplasm in these QPM hybrids is a consequential difficulty and that in their
parental inbred lines at least one more backcross with temperate germplasm should be done to select better adapted QPM.",
publisher = "Chile : Instituto de Investigaciones Agropecuarias, INIA",
journal = "Chilean Journal of Agricultural Research",
title = "Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality",
volume = "80",
number = "4",
pages = "598-607",
doi = "10.4067/S0718-58392020000400598"
}
Ignjatović-Micić, D., Kostadinović, M., Božinović, S., Đorđević-Melnik, O., Stanković, G., Delić, N.,& Vančetović, J.. (2020). Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality. in Chilean Journal of Agricultural Research
Chile : Instituto de Investigaciones Agropecuarias, INIA., 80(4), 598-607.
https://doi.org/10.4067/S0718-58392020000400598
Ignjatović-Micić D, Kostadinović M, Božinović S, Đorđević-Melnik O, Stanković G, Delić N, Vančetović J. Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality. in Chilean Journal of Agricultural Research. 2020;80(4):598-607.
doi:10.4067/S0718-58392020000400598 .
Ignjatović-Micić, Dragana, Kostadinović, Marija, Božinović, Sofija, Đorđević-Melnik, Olivera, Stanković, Goran, Delić, Nenad, Vančetović, Jelena, "Evaluation of temperate quality protein maize (Qpm) hybrids for field performance and grain quality" in Chilean Journal of Agricultural Research, 80, no. 4 (2020):598-607,
https://doi.org/10.4067/S0718-58392020000400598 . .
2
1

Morphological and physiological response of maize seedlings to chilling stress

Nikolić, Ana; Kravić, Natalija; Ristić, Danijela; Anđelković, Violeta; Marković, Ksenija; Vančetović, Jelena; Ignjatović-Micić, Dragana

(Beograd : Društavo genetičara Srbije, 2020)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kravić, Natalija
AU  - Ristić, Danijela
AU  - Anđelković, Violeta
AU  - Marković, Ksenija
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/814
AB  - Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing.
AB  - Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve
PB  - Beograd : Društavo genetičara Srbije
T2  - Genetika
T1  - Morphological and physiological response of maize seedlings to chilling stress
VL  - 52
IS  - 2
SP  - 689
EP  - 698
DO  - 10.2298/GENSR2002689N
ER  - 
@article{
author = "Nikolić, Ana and Kravić, Natalija and Ristić, Danijela and Anđelković, Violeta and Marković, Ksenija and Vančetović, Jelena and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Although accompanied with adverse low temperatures, early maize sowing could be
used to avoid drought during flowering and diminish yield losses. Herein, a small-scale
experiment of low temperature stress (LTS) on maize lines L1 (tolerant), L2 (medium
tolerant) and L3 (susceptible) is presented. Plants were grown in pots exposed to
exterior suboptimal (March) and optimal (late April) temperatures until three leaf stage.
Chlorophyll (CH), flavonoids (FL), anthocyanins (AN) and nitrogen balance (NBI)
indices were measured using Dualex Scientific optical device. Growth parameters were
also determined. Under LTS, number of plants was unchanged for L1 and halved for L2
and L3. Compared to L2 and L3, L1 had significantly higher (p<0.05) shoot fresh
weight (0.649 g vs. 0.406 g and 0.303 g), AN (0.17 vs. 0.13) and FL (1.47 vs. 1.38 and
1.36). For recovery evaluation, plants were transplanted into the field. Transplanted
stressed L1 plants showed the highest grain yield per plant (55g) in the field. Due to
high correlations (p<0.01) between FL in three leaf stage and grain yield per plant, FL
could be used as an indicator of plant recovery of maize genotypes exposed to LTS
during early sowing., Ranom setvom kukuruza se može izbeći efekat suše u fazi cvetanja i tako preduprediti smanjenje
prinosa uprkos nepovoljnim temperaturama u tom periodu. U ovom istraživanju prezentovani su
rezultati efekta niskih temperatura na tri linije kukuruza: L1 (tolerantna), L2 (srednje osetljiva) i
L3 (osetljiva). Biljke su gajene do faze trećeg lista u saksijama izloženim suboptimalnim (mart) i
optimalnim (april) spoljnim temperaturama. Sadržaj hlorofila, flavonoida i antocijana kao i
nitrogen balance index (NBI) su mereni korišćenjem uređaja Dualex Scientific (Force-A, Orsay,
France). Takođe, mereni su i parametri rasta. U poređenju sa L2 i L3, L1 je imao značajno veću
(p<0.05) svežu masu nadzemnog dela biljke (0.649 g vs. 0.406 g i 0.303 g), antocijana (0.17 vs.
0.13) i flavonoida (1.47 vs. 1.38 i 1.36). Biljke su presađene u polje radi procene oporavka.
Presađene biljke genotipa L1 su pokazale najveći prinos po biljci u polju (55g). S obzirom na
visoku korelaciju između sadržaja flavonoida u fazi trećeg lista i prinosa po biljci (p<0.01),
flavonoidi mogu biti korišćeni kao indikator oporavka biljke kukuruza izloženih niskim
temperaturama u fazi rane setve",
publisher = "Beograd : Društavo genetičara Srbije",
journal = "Genetika",
title = "Morphological and physiological response of maize seedlings to chilling stress",
volume = "52",
number = "2",
pages = "689-698",
doi = "10.2298/GENSR2002689N"
}
Nikolić, A., Kravić, N., Ristić, D., Anđelković, V., Marković, K., Vančetović, J.,& Ignjatović-Micić, D.. (2020). Morphological and physiological response of maize seedlings to chilling stress. in Genetika
Beograd : Društavo genetičara Srbije., 52(2), 689-698.
https://doi.org/10.2298/GENSR2002689N
Nikolić A, Kravić N, Ristić D, Anđelković V, Marković K, Vančetović J, Ignjatović-Micić D. Morphological and physiological response of maize seedlings to chilling stress. in Genetika. 2020;52(2):689-698.
doi:10.2298/GENSR2002689N .
Nikolić, Ana, Kravić, Natalija, Ristić, Danijela, Anđelković, Violeta, Marković, Ksenija, Vančetović, Jelena, Ignjatović-Micić, Dragana, "Morphological and physiological response of maize seedlings to chilling stress" in Genetika, 52, no. 2 (2020):689-698,
https://doi.org/10.2298/GENSR2002689N . .
2
2

Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions

Vančetović, Jelena; Kostadinović, Marija; Božinović, Sofija; Nikolić, Ana; Vukadinović, Jelena; Marković, Ksenija; Ignjatović-Micić, Dragana

(Beograd : Društvo genetičara Srbije, 2020)

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Kostadinović, Marija
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Vukadinović, Jelena
AU  - Marković, Ksenija
AU  - Ignjatović-Micić, Dragana
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/791
AB  - Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins.
AB  - Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika
T1  - Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions
T1  - Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena
VL  - 52
IS  - 1
SP  - 273
EP  - 289
DO  - 10.2298/GENSR2001273V
ER  - 
@article{
author = "Vančetović, Jelena and Kostadinović, Marija and Božinović, Sofija and Nikolić, Ana and Vukadinović, Jelena and Marković, Ksenija and Ignjatović-Micić, Dragana",
year = "2020",
abstract = "Nutritional  quality  of  maize  is  low  because  maize  protein  is  poor  in  several  essential amino  acids.  The  purpose  of  this  research  was  to  analyze  agronomic  traits  and  kernel biochemical and physical properties of 16 gene bank accessions which comprise a mini-core  collection  for  grain  quality  and  to  identify  populations  for  improving  protein quality.  Standard  ZP341  hybrid  was  superior  for  half  of  agronomic  traits  tested, especially  grain  yield,  which  was  higher  from  24%  to  six  times.  Ten  accessions  had protein  content  over  14  %  and  were  further  analyzed  for  amino  acid  composition  and kernel  characteristics.  Additionally,  genetic  relationships  between  the  accessions  were determined   by   Simple   Sequence   Repeats   (SSRs)   analysis   with   30   primers.   All accessions  showed  elevated  contents  of  most  essential  amino  acids.  Population  L492 with  1.87  and  0.68  g  100g-1dry  weight  had  the  highest  contents  of  leucine  and phenylalanine, respectively, but also higher contents of most other analyzed amino acids (p<0.05). Cluster analysis based on SSRs also distinguished L492 by separating it from all other accessions. Compared to ZP341, accessions were significantly inferior in grain weight  and  dimensions  (p<0.05),  but  superior  in  most  hardness  parameters  (p<0.05). Pearson  correlations  revealed  lack  of  negative  correlations  between  biochemical  traits, indicating  a  possibility  for  concurrent  improvement  of  several  amino  acids.  The  best way  of  improving  protein  quality  of  elite  materials  is  through  backcrossing  and  as populations  were  chosen  according  to  their  good  general  combining  ability  (with IoDent,  Lancaster  and  BSSS),  they  could  serve  for  improvement  of  elite  materials  of these genetic origins., Nutritivna vrednost kukuruza je niska zbog nedostatka nekoliko esencijalnih amino kiselina. Cilj ovog  rada  jebio da se analiziraju agronomske osobine, sadržaj proteina i amino kiselina kao i fiziĉka  svojstva  zrna  16  uzoraka  iz  banke  gena  koji  ĉine  mini coreza  kvalitet,  da  bi  se identifikovale populacije za poboljšanje kvaliteta proteina kukuruza. Hibrid ZP 341 (standard) je bio superioran za većinu testiranih agronomskih svojstava, sa prinosom zrna većim za 24% do 600%. Deset uzoraka kod kojih je sadržaj proteina bio veći od 14% je analizirano na sadržaj aminokiselina i karakteristike zrna. TakoĊe su utvrĊeni genetiĉki odnosi izmeĊu uzoraka pomoću 30  SSR  markera.  Svi  uzorci  su  pokazali  povećan  sadržaj  većine  esencijalnih  amino  kiselina. Populacija L492 je imala najveći sadržaj leucina (1.87g 100g-1suve  mase)  i  fenilalanina  (0.68g 100g-1suve  mase),  ali  i  veće  sadržaje  ostalih  aminokiselina  (p<0.05)  u  odnosu  na  ZP341  i analizirane  populacije.  Klaster  analiza  zasnovana  na  SSR  markerima  je  takoĊe  izdvojila populaciju  L492  od  svih  ostalih  populacija.  U  odnosu  na  ZP  341,  populacije  iz  banke  gena  su bile  inferiorne  u  masi  i  dimenzijama  zrna  (p<0.05), ali superiorne u većini parametara tvrdoće zrna   (p<0.05).  Pirsonove   korelacije  su  pokazale  nedostatak  negativnih  korelacija  izmeĊu analiziranih  biohemijskih  svojstava,  što  ukazuje  na  mogućnost  poboljšanja  kukuruza  na  više amino kiselina istovremeno. Najbolji naĉin poboljšanja kvaliteta proteina elitnog materijala je putem   povratnih  ukrštanja,  a  kako  su  populacije  izabrane  prema  svojim  dobrim  opštim kombinacionim sposobnostima (sa IoDent, Lancaster i BSSS), mogle bi da služe za poboljšanje elitnog materijala navedenih heterotiĉnih grupa.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika",
title = "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions, Agronomska, biohemijska i genetička svojstva populacija kukuruza visokog kvaliteta proteina iz banke gena",
volume = "52",
number = "1",
pages = "273-289",
doi = "10.2298/GENSR2001273V"
}
Vančetović, J., Kostadinović, M., Božinović, S., Nikolić, A., Vukadinović, J., Marković, K.,& Ignjatović-Micić, D.. (2020). Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika
Beograd : Društvo genetičara Srbije., 52(1), 273-289.
https://doi.org/10.2298/GENSR2001273V
Vančetović J, Kostadinović M, Božinović S, Nikolić A, Vukadinović J, Marković K, Ignjatović-Micić D. Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions. in Genetika. 2020;52(1):273-289.
doi:10.2298/GENSR2001273V .
Vančetović, Jelena, Kostadinović, Marija, Božinović, Sofija, Nikolić, Ana, Vukadinović, Jelena, Marković, Ksenija, Ignjatović-Micić, Dragana, "Agronomic, biochemical and genetic attributes of maizehigh grain quality accessions" in Genetika, 52, no. 1 (2020):273-289,
https://doi.org/10.2298/GENSR2001273V . .

Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Mladenović-Drinić, Snežana

(London : Insight Medical Publishing, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/970
AB  - Worldwide, modern grain breeding programs widely implement marker assisted selection (MAS). Molecular markers are being used in two ways: to control the target gene (foreground selection) and to accelerate the reconstruction of the recurrent parent genotype (background selection). Marker analysis allow breeders to discard plants without allels of interest prior to pollination, reducing the size of breeding population. Marker assisted selection for the qualitative traits, which are regulated by the action of a single or several genes and are clearly phenotypically defined, achieved the best results. The improvement of β-carotene content using crtRB1-specific molecular marker is one of the successful examples of MAS. The introgressed inbreds possessing favourable allele of crtRB1 and improved hybrids with increased β-carotene can be used in the biofortification program.
The results presented herein are a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions. The utility of SSR marker specific to the crtRB1 gene in foreground selection has been already tested on parental lines and distinct marker polymorphism was observed between the donor and recurrent parents. The objective of this study was to validate the ability of the same SSR marker to detect homozygous dominant and heterozygous plants separately in BC1 generation. Heterozygous individuals were clearly distinguishable, therefore they will be selected and backcrossed with the recurrent parent to produce BC2 generation. Percentage of heterozygous plants identified in BC1 generation was approximately 50%, which is in accordance with the expected Mendelian ratio of 1 O2O2 : 1 O2o2. The use of the crtRB1-specific marker will be extended in the next generation of MAS for β-carotene enriched lines for growing in temperate regions.
PB  - London : Insight Medical Publishing
C3  - 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research
T1  - Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation
VL  - 09
SP  - 27
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Mladenović-Drinić, Snežana",
year = "2019",
abstract = "Worldwide, modern grain breeding programs widely implement marker assisted selection (MAS). Molecular markers are being used in two ways: to control the target gene (foreground selection) and to accelerate the reconstruction of the recurrent parent genotype (background selection). Marker analysis allow breeders to discard plants without allels of interest prior to pollination, reducing the size of breeding population. Marker assisted selection for the qualitative traits, which are regulated by the action of a single or several genes and are clearly phenotypically defined, achieved the best results. The improvement of β-carotene content using crtRB1-specific molecular marker is one of the successful examples of MAS. The introgressed inbreds possessing favourable allele of crtRB1 and improved hybrids with increased β-carotene can be used in the biofortification program.
The results presented herein are a part of the breeding program conducted at the Maize Research Institute "Zemun Polje", with the aim to convert the standard maize to β-carotene rich genotypes adapted to temperate regions. The utility of SSR marker specific to the crtRB1 gene in foreground selection has been already tested on parental lines and distinct marker polymorphism was observed between the donor and recurrent parents. The objective of this study was to validate the ability of the same SSR marker to detect homozygous dominant and heterozygous plants separately in BC1 generation. Heterozygous individuals were clearly distinguishable, therefore they will be selected and backcrossed with the recurrent parent to produce BC2 generation. Percentage of heterozygous plants identified in BC1 generation was approximately 50%, which is in accordance with the expected Mendelian ratio of 1 O2O2 : 1 O2o2. The use of the crtRB1-specific marker will be extended in the next generation of MAS for β-carotene enriched lines for growing in temperate regions.",
publisher = "London : Insight Medical Publishing",
journal = "5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research",
title = "Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation",
volume = "09",
pages = "27"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D.,& Mladenović-Drinić, S.. (2019). Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation. in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research
London : Insight Medical Publishing., 09, 27.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Mladenović-Drinić S. Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation. in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research. 2019;09:27..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Mladenović-Drinić, Snežana, "Marker assisted selection for β-carotene rich maize: validation of the gene-specific marker in BC1 generation" in 5. Edition of international conference on plant genomics, 13-14.06.2019., Berlin, Germany, Asian journal of plant science & research, 09 (2019):27.

The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version

Kostadinović, Marija; Ristić, Danijela; Kovinčić, Anika; Simić, Marijana; Perić, Vesna; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Belgrade : Serbian Genetic Society, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ristić, Danijela
AU  - Kovinčić, Anika
AU  - Simić, Marijana
AU  - Perić, Vesna
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/963
AB  - Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.
PB  - Belgrade : Serbian Genetic Society
C3  - 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
T1  - The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version
SP  - 23
EP  - 30
ER  - 
@conference{
author = "Kostadinović, Marija and Ristić, Danijela and Kovinčić, Anika and Simić, Marijana and Perić, Vesna and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Quality Protein Maize (QPM) is nutritionally and agronomically improved maize. In order to shorten the period required for development of QPM hybrids through the conventional method of backcrossing, marker assisted selection (MAS) is being used. After successful conversion of one commercial maize inbred line to its QPM counterpart for growing in temperate climate, this breeding program was continued at Maize Research Institute (MRI) with larger number of maize lines. Four commercial MRI inbreds, chosen for marker assisted introgression of the quality protein trait, and their BC2 progenies were subjected to two-level selection procedure. First, BC2 plants were analyzed with opaque2 (o2) specific molecular markers to identify heterozygotes. Second, the selected heterozygotes were screened with SSR markers distributed throughout the genome to identify genotypes with the highest recovery of recurrent parent’s genome (RPG). The specific markers identified 100 out of 192 plants (52%) as heterozygous. Genetic similarity values between parental lines and their BC2 heterozygous progenies were in the range from 0.77 to 0.99 (77-99% RPG). The highest proportion of RPG was found in L1 (93-99%) and the lowest in L3 progenies (77-89%). Average values for the RPG content ranged from 83.9 to 95.8%. Progenies with RPG above 95% were selfed to produce BC2F2 plants which will be subjected to foreground selection. This time selection will be focused on homozygous recessive individuals, given that the presence of opaque2 gene in the homozygous recessive state is the aim of the QPM selection. Finally, those o2o2 genotypes will be screened for biochemical and phenotypic traits to confirm their nutritional and agronomical superiority.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings",
title = "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version",
pages = "23-30"
}
Kostadinović, M., Ristić, D., Kovinčić, A., Simić, M., Perić, V., Ignjatović-Micić, D.,& Vančetović, J.. (2019). The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings
Belgrade : Serbian Genetic Society., 23-30.
Kostadinović M, Ristić D, Kovinčić A, Simić M, Perić V, Ignjatović-Micić D, Vančetović J. The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version. in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings. 2019;:23-30..
Kostadinović, Marija, Ristić, Danijela, Kovinčić, Anika, Simić, Marijana, Perić, Vesna, Ignjatović-Micić, Dragana, Vančetović, Jelena, "The two-level marker assisted selection in BC2 generation of the conversion of standard maize lines to their QPM version" in 6. Congress of the Serbian genetic society, 13-17.10.2019., Vrnjačka Banja, Serbia - Book of proceedings (2019):23-30.

Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Obradović, Ana; Đorđević-Melnik, Olivera; Kovinčić, Anika

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2019)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Đorđević-Melnik, Olivera
AU  - Kovinčić, Anika
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/964
AB  - Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
T1  - Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines
SP  - 91
EP  - 95
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Đorđević-Melnik, Olivera and Kovinčić, Anika",
year = "2019",
abstract = "Marker assisted selection (MAS) significantly increases efficiency of conventional breeding. Molecular markers are utilized as selection markers for target genes (foreground selection) and also for identification of the genotypes (progenies) with the highest proportion of recurrent parent’s genome (background selection). Maize Research Institute "Zemun Polje" has a breeding program with the aim to create lines with incorporated incompatibility dominant gene Gametophytic Factor 1-S (Gа1-S), using the integrated conventional and molecular breeding approach. Ga1-S is the most described gene belonging to the group of genes specific to the pollen development, germination and pollen tube growth. The Ga1-S system is the most commonly used to prevent the pollination of sweetcorn, popcorn and white kerneld hybrids by standard maize. The objectives of this study were identification of gene-specific molecular marker for foreground selection, as well as the set of SSR markers polymorphic between parental lines to be used in background selection. Genetic variability between two donor and three recurrent parental inbred lines was analyzed with 42 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 83, average being 2.77. The genetic similarity values calculated on Dice coefficient ranged from 0.47 to 0.71. Among 12 gene-specific markers tested on parental lines, two showed distinct polymorphism for Ga1-S. These markers will be used as foreground selection markers for the incorporation of Gа1-S into our inbred lines which will be used for the creation of white kernel hybrids.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings",
title = "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines",
pages = "91-95"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Đorđević-Melnik, O.,& Kovinčić, A.. (2019). Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 91-95.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Đorđević-Melnik O, Kovinčić A. Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines. in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings. 2019;:91-95..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Đorđević-Melnik, Olivera, Kovinčić, Anika, "Identification of molecular markers for foreground and background selection in Gа1-s incorporation into maize lines" in 10. international scientific agriculture symposium "Agrosym 2019", 03-06.10.2019., Jahorina, B i H - Book of proceedings (2019):91-95.

Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress

Dodig, Dejan; Božinović, Sofija; Nikolić, Ana; Zorić, Miroslav; Vančetović, Jelena; Ignjatović-Micić, Dragana; Delić, Nenad; Weigelt-Fischer, Kathleen; Junker, Astrid; Altmann, Thomas

(Lausanne : Frontiers Media SA, 2019)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Zorić, Miroslav
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Weigelt-Fischer, Kathleen
AU  - Junker, Astrid
AU  - Altmann, Thomas
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/812
AB  - Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.
PB  - Lausanne : Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress
VL  - 10
SP  - 814
DO  - 10.3389/fpls.2019.00814
ER  - 
@article{
author = "Dodig, Dejan and Božinović, Sofija and Nikolić, Ana and Zorić, Miroslav and Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Weigelt-Fischer, Kathleen and Junker, Astrid and Altmann, Thomas",
year = "2019",
abstract = "Phenotypic measurements under controlled cultivation conditions are essential to gain
a mechanistic understanding of plant responses to environmental impacts and thus
for knowledge-based improvement of their performance under natural field conditions.
Twenty maize inbred lines (ILs) were phenotyped in response to two levels of water and
nitrogen supply (control and stress) and combined nitrogen and water deficit. Over a
course of 5 weeks (from about 4-leaf stage to the beginning of the reproductive stage),
maize phenology and growth were monitored by using a high-throughput phenotyping
platform for daily acquisition of images in different spectral ranges. The focus of the
present study is on the measurements taken at the time of maximum water stress (for
traits that reflect plant physiological properties) and at the end of the experiment (for
traits that reflect plant architectural and biomass-related traits). Twenty-five phenotypic
traits extracted from the digital image data that support biological interpretation of
plant growth were selected for their predictive value for mid-season shoot biomass
accumulation. Measured fresh and dry weights after harvest were used to calculate
various indices (water-use efficiency, physiological nitrogen-use efficiency, specific plant
weight) and to establish correlations with image-derived phenotypic features. Also, score
indices based on dry weight were used to identify contrasting ILs in terms of productivity
and tolerance to stress, and their means for image-derived and manually measured traits
were compared. Color-related traits appear to be indicative of plant performance and
photosystem II operating efficiency might be an importance physiological parameter
of biomass accumulation, particularly under severe stress conditions. Also, genotypes
showing greater leaf area may be better adapted to abiotic stress conditions.",
publisher = "Lausanne : Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress",
volume = "10",
pages = "814",
doi = "10.3389/fpls.2019.00814"
}
Dodig, D., Božinović, S., Nikolić, A., Zorić, M., Vančetović, J., Ignjatović-Micić, D., Delić, N., Weigelt-Fischer, K., Junker, A.,& Altmann, T.. (2019). Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science
Lausanne : Frontiers Media SA., 10, 814.
https://doi.org/10.3389/fpls.2019.00814
Dodig D, Božinović S, Nikolić A, Zorić M, Vančetović J, Ignjatović-Micić D, Delić N, Weigelt-Fischer K, Junker A, Altmann T. Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress. in Frontiers in Plant Science. 2019;10:814.
doi:10.3389/fpls.2019.00814 .
Dodig, Dejan, Božinović, Sofija, Nikolić, Ana, Zorić, Miroslav, Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Weigelt-Fischer, Kathleen, Junker, Astrid, Altmann, Thomas, "Image-derived traits related to mid-season growth performance of maize under nitrogen and water stress" in Frontiers in Plant Science, 10 (2019):814,
https://doi.org/10.3389/fpls.2019.00814 . .
7
8
5

Marker assisted backcross breeding in Maize Research Institute Zemun Polje

Kostadinović, Marija; Nikolić, Ana; Ristić, Danijela; Božinović, Sofija; Đorđević-Melnik, Olivera; Ignjatović-Micić, Dragana; Vančetović, Jelena

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2019)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Božinović, Sofija
AU  - Đorđević-Melnik, Olivera
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/743
AB  - Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije.
AB  - The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Marker assisted backcross breeding in Maize Research Institute Zemun Polje
T1  - Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje
VL  - 25
IS  - 1
SP  - 41
EP  - 47
DO  - 10.5937/SelSem1901041K
UR  - conv_2003
ER  - 
@article{
author = "Kostadinović, Marija and Nikolić, Ana and Ristić, Danijela and Božinović, Sofija and Đorđević-Melnik, Olivera and Ignjatović-Micić, Dragana and Vančetović, Jelena",
year = "2019",
abstract = "Povratno ukrštanje je pristup u klasičnom oplemenjivanju koji se koristi za unošenje jednog ili više poželjnih alela, na jedan ili više lokusa, iz donora u adaptiranu ili komercijalnu liniju (rekurentni roditelj). U poslednjih nekoliko decenija, zahvaljujući razvoju metoda molekularne genetike razvija se oplemenjivanje potpomognuto molekularnim markerima. U cilju bržeg dobijanja željenih genotipova (potomstvo koje nosi poželjni gen sa maksimalnim povraćajem genoma rekurentnog roditelja) primenjuje se kombinacija povratnog ukrštanja i selekcije pomoću SSR molekularnih markera. Za ovo istraživanje je odabrano dvanaest samooplodnih linija kukuruza (L1 -L12) u cilju unošenja poželjnih alela uključenih u ekspresiju svojstava: restorer za CMS-C (RfC), restorer za CMS-S (RfS), za plavu boju zrna i za crvenu boju perikarpa. Samooplodne linije i njhovo BC4 potomstvo su analizirani SSR molekularnim markerima u cilju identifikacije genotipova sa najvećim procentom genoma rekurentnog roditelja. Za analizu je odabrano 30 SSR markera raspoređenih na svih 10 hromozoma kukuruza. Vrednosti genetičke sličnosti između rekurentnih roditelja i njihovih BC4 potomstava su bile u opsegu od 0.79 do0.99 (79-99% genoma rekurentnog roditelja). Rezultati su pokazali da je 48% potomstva imalo povraćaj genoma rekurentnog roditelja iznad teoretske vrednosti i ono je podvrgnuto procesu samooplodnje u cilju fiksacije alela. Takođe, kod 41% potomstva povraćaj je iznosio 99%, što predstavlja vrednost koja se teoretski dostiže u BC6 generaciji. Sa druge strane, 52% potomstva je imalo manji povraćaj genoma rekurentnog roditelja od teorijskog. U ovom istraživanju prikazana je praktična primena molekularnih markera u povratnom ukrštanju i ovaj pristup se može koristiti za postizanje bolje efikasnosti i skraćivanje procesa selekcije., The backcross breeding is one of the most extensively used methods in different crop species. With the development of molecular genetics, molecular markers become widely used as a tool aiding conventional breeding. The main goal of marker assisted backcross breeding (MABB), a form of marker assisted selection (MAS), is to develop backcross progenies carrying the gene of interest with maximum recovery of the recurrent parent's genome (RPG). In this research, twelve inbred lines were chosen for the introgression of favourable alleles for several traits. Thirty SSR markers distributed throughout the whole genome were used to determine genetic similarity among these twelve lines and their BC4 progenies, i.e.to identify the genotypes with the highest proportion of recurrent parent's genome (RPG). Genetic similarity values ranged from 0.79 to 0.99(79-99% RPG).The 48% of progenies had RPG above theoretical value and they were self-pollinated for allele fixation. Also, 41% of them had 99%, what is the value theoretically achieved in BC6 generation. On the other hand, 52% of individuals had lower RPG content than theory predicts. The results of the research confirmed the advantage of using MABB compared to conventional approach, providing faster achievement of the goal, recovering the recurrent parent's genome in fewer generations of backcrossing and thus enabled enhanced selection efficiency and shortened the breeding process.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Marker assisted backcross breeding in Maize Research Institute Zemun Polje, Primena molekularnih markera kod povratnih ukrštanja u Institutu za kukuruz Zemun Polje",
volume = "25",
number = "1",
pages = "41-47",
doi = "10.5937/SelSem1901041K",
url = "conv_2003"
}
Kostadinović, M., Nikolić, A., Ristić, D., Božinović, S., Đorđević-Melnik, O., Ignjatović-Micić, D.,& Vančetović, J.. (2019). Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 25(1), 41-47.
https://doi.org/10.5937/SelSem1901041K
conv_2003
Kostadinović M, Nikolić A, Ristić D, Božinović S, Đorđević-Melnik O, Ignjatović-Micić D, Vančetović J. Marker assisted backcross breeding in Maize Research Institute Zemun Polje. in Selekcija i semenarstvo. 2019;25(1):41-47.
doi:10.5937/SelSem1901041K
conv_2003 .
Kostadinović, Marija, Nikolić, Ana, Ristić, Danijela, Božinović, Sofija, Đorđević-Melnik, Olivera, Ignjatović-Micić, Dragana, Vančetović, Jelena, "Marker assisted backcross breeding in Maize Research Institute Zemun Polje" in Selekcija i semenarstvo, 25, no. 1 (2019):41-47,
https://doi.org/10.5937/SelSem1901041K .,
conv_2003 .
3

Parental polymorphism analysis in marker assisted selection for β-carotene rich maize

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Ristić, Danijela; Obradović, Ana; Stevanović, Milan; Mladenović-Drinić, Snežana

(Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia, 2018)

TY  - CONF
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Ristić, Danijela
AU  - Obradović, Ana
AU  - Stevanović, Milan
AU  - Mladenović-Drinić, Snežana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/960
AB  - Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.
PB  - Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
T1  - Parental polymorphism analysis in marker assisted selection for β-carotene rich maize
SP  - 333
EP  - 338
ER  - 
@conference{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Ristić, Danijela and Obradović, Ana and Stevanović, Milan and Mladenović-Drinić, Snežana",
year = "2018",
abstract = "Marker assisted selection (MAS) is widely implemented into modern grain breeding programs. Molecular markers are used in foreground selection to control the target gene, as well as in background selection to accelerate the reconstruction of the recurrent parent genotype. The best results have been achieved with the qualitative traits, regulated by the action of a single or several genes and clearly phenotypically defined. One successful example of MAS is the improvement of β-carotene content using crtRB1 specific molecular marker. Maize Research Institute "Zemun Polje" has a breeding program aimed at conversion of standard maize to β-carotene rich genotypes adapted to temperate regions. The objectives of this study were to test the utility of gene-specific SSR marker in foreground selection and to identify polymorphic markers between parental lines to be used in background selection. Genetic variability between two standard and three high β-carotene parental inbred lines was analyzed with 40 SSRs distributed over the maize genom. Total number of alleles detected with 30 informative markers was 77, average being 2.57. The genetic similarity values calculated on Dice coefficient ranged from 0.49 to 0.66. Parental polymorphism for crtRB1 showed a 543 bp fragment in donor lines, whereas a distinct 296 bp amplicon and a faint 1221 bp amplicon were generated in the recurrent parents. This marker will be used as foreground selection marker for the crtRB1 gene in the conversion of standard maize to β-carotene enriched lines for growing in temperate regions.",
publisher = "Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings",
title = "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize",
pages = "333-338"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Ristić, D., Obradović, A., Stevanović, M.,& Mladenović-Drinić, S.. (2018). Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings
Sarajevo : University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 333-338.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Ristić D, Obradović A, Stevanović M, Mladenović-Drinić S. Parental polymorphism analysis in marker assisted selection for β-carotene rich maize. in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings. 2018;:333-338..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Ristić, Danijela, Obradović, Ana, Stevanović, Milan, Mladenović-Drinić, Snežana, "Parental polymorphism analysis in marker assisted selection for β-carotene rich maize" in 9. international scientific agriculture symposium "Agrosym 2018" 04-07.10.2018., Jahorina, B i H - Book of proceedings (2018):333-338.

Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna

Kostadinović, Marija; Ignjatović-Micić, Dragana; Vančetović, Jelena; Stanković, Goran; Delić, Nenad

(Beograd : Društvo genetičara Srbije, 2018)

TY  - GEN
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Delić, Nenad
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/961
AB  - Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića.
AB  - Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
T2  - 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
T1  - Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna
T1  - Marker assisted selection for improving protein quality of maize grain
SP  - 170
EP  - 171
ER  - 
@misc{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Vančetović, Jelena and Stanković, Goran and Delić, Nenad",
year = "2018",
abstract = "Molekularna genetika je danas sastavni deo različitih programa selekcije i oplemenjivanja žitarica u svetu. Najveći napredak u oplemenjivanju primenom molekularnih markera (eng. Marker Assisted Selection - MAS) postignut je kod kvalitativnih osobina koje su regulisane dejstvom jednog ili nekoliko gena i jasno su fenotipski definisane. Molekularni markeri se koriste kao selekcioni markeri za željeni gen (foreground selection), kao i radi utvrđivanja procenta genoma rekurentnog roditelja u potomstvima povratnih ukrštanja (background selection). Primena foreground i background selekcije smanjuje broj generacija potrebnih za stvaranje željenog genotipa klasičnim metodama selekcije koje se zasnivaju na fenotipskom odabiru biljaka.
Jedan od primera uspešne primene MAS je povećanje hranljive vrednosti zrna kukuruza. Upotrebom molekularnih markera specifičnih za ciljne gene opaque2, crtRB1 i lpa2-2 dobijaju se linije kukuruza visokog kvaliteta proteina, visokog sadržaja beta karotena i niskog sadržaja fitina, redom. Dobijene linije koriste se za stvaranje agronomski superiornih hibrida povećane hranljive i biološke vrednosti.
U Institutu za kukuruz "Zemun Polje" razvijen je program stvaranja genotipova kukuruza visokog kvaliteta proteina (eng. Quality Protein Maize - QPM) adaptiranih na umereno klimatsko područje kombinovanim pristupom klasične selekcije i selekcije pomoću molekularnih markera. Jedna komercijalna linija prevedena je na QPM varijantu, korišćenjem tropske linije CML 144 kao donora poželjnih svojstava, u procesu koji se sastojao iz dva ciklusa povratnog ukrštanja i tri ciklusa samooplodnje. Ukrštanjem ove prevedene linije sa američkim QPM linijama dobijeni su hibridi poboljšanog kvaliteta proteina namenjeni ishrani tovnih pilića i prasića., Molecular genetic technologies are implemented into modern grain breeding programs in the world. The greatest progress in marker assisted selection (MAS) has been achieved with the qualitative traits that are regulated by the action of a single or several genes and are clearly phenotypically defined. Markers can be used to either control the target gene (foreground selection) or to accelerate the reconstruction of the recurrent parent genotype (background selection). Both foreground and background selection decrease the number of the generations required to create desirable genotype through conventional breeding based on the fenotypic selection. 
One successful example of MAS application is the improvement of the maize nutritional value. Using specific molecular markers for desirable genes opaque2, crtRB1 and lpa2-2, maize lines with enhanced protein quality, enhanced β-carotene content and low phytic acid content are developed, respectively. These lines are being used to obtain high-yielding hybrids with improved nutritional benefit. 
Breeding program for developing quality protein maize (QPM) adapted to temperate regions through marker assisted selection is being conducted at Maize Research Institute Zemun Polje. One commercial inbred line was converted to its QPM counterpart, using tropical QPM line CML 144 as a donor line, after two generations of backcrossing and three generations of selfing. By crossing this converted line with American QPM lines, the hybrids with enhanced protein quality aimed for broiler and piglets feeding are obtained.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata",
title = "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna, Marker assisted selection for improving protein quality of maize grain",
pages = "170-171"
}
Kostadinović, M., Ignjatović-Micić, D., Vančetović, J., Stanković, G.,& Delić, N.. (2018). Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata
Beograd : Društvo genetičara Srbije., 170-171.
Kostadinović M, Ignjatović-Micić D, Vančetović J, Stanković G, Delić N. Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna. in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata. 2018;:170-171..
Kostadinović, Marija, Ignjatović-Micić, Dragana, Vančetović, Jelena, Stanković, Goran, Delić, Nenad, "Primena molekularnih markera za selekciju kukuruza poboljšanog kvaliteta proteina zrna" in 6. Simpozijum Sekcije za oplemenjivanje organizama Društva Genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, 07-11.05.2018., Vrnjačka Banja, Srbija - Zbornik apstrakata (2018):170-171.

Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel

Čamdžija, Zoran; Dragičević, Vesna; Vančetović, Jelena; Stevanović, Milan; Pavlov, Jovan; Filipović, Milomir; Ignjatović-Micić, Dragana

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Čamdžija, Zoran
AU  - Dragičević, Vesna
AU  - Vančetović, Jelena
AU  - Stevanović, Milan
AU  - Pavlov, Jovan
AU  - Filipović, Milomir
AU  - Ignjatović-Micić, Dragana
PY  - 2018
UR  - http://rik.mrizp.rs/handle/123456789/727
AB  - A set of fifteen elite inbred lines of maize (used as mothers) and three tester inbred lines (used as fathers) were investigated using line x tester statistical model, including both hybrids and inbreds. Four traits were measured: grain yield, 1000 kernel weight, phytic phosphorus (Pphy) and inorganic phosphorus (P-i) in the kernel. P-phy content among hybrids ranged from 2.342 to 4.812 g kg(-1) and P-i content from 0.562 to 2.340 g kg(-1), while among inbreds (lines and testers) they ranged from 2.503 to 4.180 g kg(-1) and from 0.587 to 1.629 g kg(-1), respectively. Correlations between the four traits allow breeding for high P, and low Pphy, as well as for both high P-i and phytate, without compromising grain yield. Correlation for hybrids between P-phy and P-i was 0.185 (p lt 0.05) and for inbreds 0.142 (non-significant). General combining ability / special combining ability (GCA/SCA) values for all the traits were below 1 (very low) indicated non-additive inheritance. In the investigated set of genotypes, multiple selection indices should be used for simultaneous improvement of grain yield and phosphorus profile of maize grain.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel
VL  - 50
IS  - 1
SP  - 299
EP  - 314
DO  - 10.2298/GENSR1801299C
UR  - conv_980
ER  - 
@article{
author = "Čamdžija, Zoran and Dragičević, Vesna and Vančetović, Jelena and Stevanović, Milan and Pavlov, Jovan and Filipović, Milomir and Ignjatović-Micić, Dragana",
year = "2018",
abstract = "A set of fifteen elite inbred lines of maize (used as mothers) and three tester inbred lines (used as fathers) were investigated using line x tester statistical model, including both hybrids and inbreds. Four traits were measured: grain yield, 1000 kernel weight, phytic phosphorus (Pphy) and inorganic phosphorus (P-i) in the kernel. P-phy content among hybrids ranged from 2.342 to 4.812 g kg(-1) and P-i content from 0.562 to 2.340 g kg(-1), while among inbreds (lines and testers) they ranged from 2.503 to 4.180 g kg(-1) and from 0.587 to 1.629 g kg(-1), respectively. Correlations between the four traits allow breeding for high P, and low Pphy, as well as for both high P-i and phytate, without compromising grain yield. Correlation for hybrids between P-phy and P-i was 0.185 (p lt 0.05) and for inbreds 0.142 (non-significant). General combining ability / special combining ability (GCA/SCA) values for all the traits were below 1 (very low) indicated non-additive inheritance. In the investigated set of genotypes, multiple selection indices should be used for simultaneous improvement of grain yield and phosphorus profile of maize grain.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel",
volume = "50",
number = "1",
pages = "299-314",
doi = "10.2298/GENSR1801299C",
url = "conv_980"
}
Čamdžija, Z., Dragičević, V., Vančetović, J., Stevanović, M., Pavlov, J., Filipović, M.,& Ignjatović-Micić, D.. (2018). Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel. in Genetika
Društvo genetičara Srbije, Beograd., 50(1), 299-314.
https://doi.org/10.2298/GENSR1801299C
conv_980
Čamdžija Z, Dragičević V, Vančetović J, Stevanović M, Pavlov J, Filipović M, Ignjatović-Micić D. Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel. in Genetika. 2018;50(1):299-314.
doi:10.2298/GENSR1801299C
conv_980 .
Čamdžija, Zoran, Dragičević, Vesna, Vančetović, Jelena, Stevanović, Milan, Pavlov, Jovan, Filipović, Milomir, Ignjatović-Micić, Dragana, "Inheritance of inorganic and phytic phosphorus in maize (Zea mays l.) Kernel" in Genetika, 50, no. 1 (2018):299-314,
https://doi.org/10.2298/GENSR1801299C .,
conv_980 .
1
1

Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia

Obradović, Ana; Stanković, Slavica; Nikolić, Ana; Ignjatović-Micić, Dragana; Krnjaja, Vesna; Stepanović, Jelena; Duduk, Bojan

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Obradović, Ana
AU  - Stanković, Slavica
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Krnjaja, Vesna
AU  - Stepanović, Jelena
AU  - Duduk, Bojan
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/682
AB  - Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia
VL  - 49
IS  - 1
SP  - 355
EP  - 364
DO  - 10.2298/GENSR1701355O
UR  - conv_957
ER  - 
@article{
author = "Obradović, Ana and Stanković, Slavica and Nikolić, Ana and Ignjatović-Micić, Dragana and Krnjaja, Vesna and Stepanović, Jelena and Duduk, Bojan",
year = "2017",
abstract = "Diversity of trichothecene chemotypes of Fusarium graminearum isolated from kernels of wheat, barley and maize grown under various agro-ecological conditions on 13 locations was analysed. Sixteen strains were tested for the effective capability to produce 15-ADON, 3-ADON and NIV, by using the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. Fourteen out of sixteen analyzed strains produced 15ADON, while remaining two were of the 3-ADON chemotype. Multiplex PCR reaction with two sets of specific primers for TRI3 and TRI12 genes was applied to identify trichothecene chemotypes (3-ADON, 15-ADON and NIV). The expected sizes of amplified fragments for TRI3 gene primer set are 840 bp (NIV), 610 bp (15-ADON) and 243 bp (3-ADON). The amplified fragments for TRI12 gene primer set should be 840 bp (NIV), 670 bp (15-ADON) and 410 bp (3-ADON). All F. graminearum isolates were of the 15-ADON chemotype, i. e. their bands were 610 bp and 670 bp size for TRI3 and TRI12 genes, respectively. The results indicate that genotypic characterisation does not correspond to determined chemotypes and this is a reason why the analyses for the risk of mycotoxins contamination should not be based only on trichotecene genotype determination. Due to high temperature differences in cereal growing regions in Serbia, the presence of other chemotypes could be expected. In order to determine whether besides 15-ADON there are other F. graminearum chemotypes on wheat, barley and maize kernels, further studies should include a large number of isolates from different agro-ecological conditions.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia",
volume = "49",
number = "1",
pages = "355-364",
doi = "10.2298/GENSR1701355O",
url = "conv_957"
}
Obradović, A., Stanković, S., Nikolić, A., Ignjatović-Micić, D., Krnjaja, V., Stepanović, J.,& Duduk, B.. (2017). Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 49(1), 355-364.
https://doi.org/10.2298/GENSR1701355O
conv_957
Obradović A, Stanković S, Nikolić A, Ignjatović-Micić D, Krnjaja V, Stepanović J, Duduk B. Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia. in Genetika. 2017;49(1):355-364.
doi:10.2298/GENSR1701355O
conv_957 .
Obradović, Ana, Stanković, Slavica, Nikolić, Ana, Ignjatović-Micić, Dragana, Krnjaja, Vesna, Stepanović, Jelena, Duduk, Bojan, "Trichothecene chemotype diversity of fusarium graminearum isolated from wheat, maize and barley in Serbia" in Genetika, 49, no. 1 (2017):355-364,
https://doi.org/10.2298/GENSR1701355O .,
conv_957 .
6
3
4

Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends

Vančetović, Jelena; Ignjatović-Micić, Dragana; Delić, Nenad; Božinović, Sofija; Nikolić, Ana; Kostadinović, Marija; Trbović, Dejana

(Univ Sao Paolo, Cerquera Cesar, 2017)

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Božinović, Sofija
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Trbović, Dejana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/684
AB  - A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.
PB  - Univ Sao Paolo, Cerquera Cesar
T2  - Scientia Agricola
T1  - Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends
VL  - 74
IS  - 6
SP  - 461
EP  - 468
DO  - 10.1590/1678-992X-2016-0302
UR  - conv_959
ER  - 
@article{
author = "Vančetović, Jelena and Ignjatović-Micić, Dragana and Delić, Nenad and Božinović, Sofija and Nikolić, Ana and Kostadinović, Marija and Trbović, Dejana",
year = "2017",
abstract = "A pilot experiment was undertaken in order to examine high oil populations of maize (Zea mays L.) to be used as pollinators in TopCross blends with commercial ZP341 standard hybrid. Five high oil populations (HOPs) from the Maize Research Institute (MRI) gene bank were chosen for this research, according to their high grain oil content, synchrony between silking of ZP341 and anthesis of the populations and good agronomic performances in 2012. Selfing of ZP341 and HOPs, as well as crosses of ZP341 cmsS sterile x HOPs were carried out in 2013. Oil content, fatty acid composition, protein and tryptophan content, and physical characteristics of the obtained kernels were measured. Four HOPs showed significant positive influence on the oil content in the TopCrosses (TC), 16.85 g kg(-1) on average. Oleic acid, which is the principal monounsaturated fatty acid, was significantly lower in all HOPs and all TCs, while selfed ZP341 had almost twice the average value typical for standard maize. However, this decrease in TCs was in a narrow range from 1 % (in TC-3) to 5 % (in TC-4) and the oleic content of TCs was on average higher by 60 % compared to the typical standard maize. Different favorable and unfavorable significant changes were detected in fatty acid compositions, protein and tryptophan contents and physical kernel properties for each potential TC combination. Results indicate differences in gene effects present in different TC combinations and underscore the need to examine each potential TC blend by conducting similar simple experiments.",
publisher = "Univ Sao Paolo, Cerquera Cesar",
journal = "Scientia Agricola",
title = "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends",
volume = "74",
number = "6",
pages = "461-468",
doi = "10.1590/1678-992X-2016-0302",
url = "conv_959"
}
Vančetović, J., Ignjatović-Micić, D., Delić, N., Božinović, S., Nikolić, A., Kostadinović, M.,& Trbović, D.. (2017). Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola
Univ Sao Paolo, Cerquera Cesar., 74(6), 461-468.
https://doi.org/10.1590/1678-992X-2016-0302
conv_959
Vančetović J, Ignjatović-Micić D, Delić N, Božinović S, Nikolić A, Kostadinović M, Trbović D. Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends. in Scientia Agricola. 2017;74(6):461-468.
doi:10.1590/1678-992X-2016-0302
conv_959 .
Vančetović, Jelena, Ignjatović-Micić, Dragana, Delić, Nenad, Božinović, Sofija, Nikolić, Ana, Kostadinović, Marija, Trbović, Dejana, "Biochemical and physical kernel properties of a standard maize hybrid in different TopCross (TM) Blends" in Scientia Agricola, 74, no. 6 (2017):461-468,
https://doi.org/10.1590/1678-992X-2016-0302 .,
conv_959 .
2
2
2

Comparison of statistical methods for genetic similarity evaluation of maize inbred lines

Nikolić, Ana; Kostadinović, Marija; Vančetović, Jelena; Stanković, Goran; Ignjatović-Micić, Dragana

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2017)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Vančetović, Jelena
AU  - Stanković, Goran
AU  - Ignjatović-Micić, Dragana
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/679
AB  - Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index.
AB  - Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Comparison of statistical methods for genetic similarity evaluation of maize inbred lines
T1  - Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza
VL  - 54
IS  - 1
SP  - 25
EP  - 30
DO  - 10.5937/ratpov54-12174
UR  - conv_623
ER  - 
@article{
author = "Nikolić, Ana and Kostadinović, Marija and Vančetović, Jelena and Stanković, Goran and Ignjatović-Micić, Dragana",
year = "2017",
abstract = "Conventional breeding methods have been aided by molecular genetic techniques giving the chance for efficient improvement in creation of maize hybrids. Proper choice of statistical methods for data analysis is very important because it ensures greater reliability. The aim of this study was to determine the most suitable statistical approach for molecular marker data analysis. SSR markers were used for the analysis of 10 maize inbreds. Genetic similarity/distance was calculated using three types of data: binary, allele frequency based on densitometry and allele frequency according to band size data applying Simple matching, Jaccard's and Rogers' coefficient. Cluster analysis was performed in NTSYS, 2.11a software. The highest value for Spearman's rank of correlation (0.95) was detected between distance matrices based on binary data. The results showed that binary data Jaccard's coefficient) and allele frequency data based on fragment sizes (Rogers' coefficient) gave identical clusters by visual inspection and according to CIc index., Metode klasične selekcije kukuruza se dopunjuju tehnikama molekularne genetike u cilju efikasnijeg dobijanja pouzdanih rezultata, pri čemu pristupi u obradi podataka imaju veliki značaj u ostvarivanju ovog cilja. Primenom SSR molekularnih markera analizirano je 10 samooplodnih linija kukuruza. Različite statističke metode su upoređene sa ciljem da se utvrdi najpogodnija za određivanje genetičke srodnosti ispitivanih genotipova. Genetička sličnost/distanca je izračunata korišćenjem tri tipa podataka: binarni podaci (1,0), frekvencija alela izračunata pomoću denzitometrije i frekvencija alela izračunata na osnovu veličina umnoženih fragmenata u baznim parovima primenom Simple matching, Jaccard i Rogers koeficijenata. Klaster analiza je urađena u NTSYS, 2.11a softveru. Najveća vrednost Spirmanovog koeficijenta (0.95) je utvrđena između matrica genetičkih sličnosti/distanci izračunatih na osnovu binarnih podataka. Rezultati ukazuju da se identični dendrogrami dobijaju korišćenjem Jaccard-ovog koeficijenta izračunatog za binarni zapis i Rogers-ovog koeficijenta izračunatog na osnovu frekvencija alela određenih prema veličini umnoženih fragmenata, kako vizuelnom ocenom tako i na osnovu CIc indeksa.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines, Poređenje statističkih metoda za određivanje genetičke srodnosti samooplodnih linija kukuruza",
volume = "54",
number = "1",
pages = "25-30",
doi = "10.5937/ratpov54-12174",
url = "conv_623"
}
Nikolić, A., Kostadinović, M., Vančetović, J., Stanković, G.,& Ignjatović-Micić, D.. (2017). Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 54(1), 25-30.
https://doi.org/10.5937/ratpov54-12174
conv_623
Nikolić A, Kostadinović M, Vančetović J, Stanković G, Ignjatović-Micić D. Comparison of statistical methods for genetic similarity evaluation of maize inbred lines. in Ratarstvo i povrtarstvo. 2017;54(1):25-30.
doi:10.5937/ratpov54-12174
conv_623 .
Nikolić, Ana, Kostadinović, Marija, Vančetović, Jelena, Stanković, Goran, Ignjatović-Micić, Dragana, "Comparison of statistical methods for genetic similarity evaluation of maize inbred lines" in Ratarstvo i povrtarstvo, 54, no. 1 (2017):25-30,
https://doi.org/10.5937/ratpov54-12174 .,
conv_623 .
1

DNA and biochemical analysis of a potential opaque2 maize population

Ignjatović-Micić, Dragana; Nikolić, Ana; Kostadinović, Marija; Nikolić, Aleksandra

(Društvo genetičara Srbije, Beograd, 2017)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Nikolić, Ana
AU  - Kostadinović, Marija
AU  - Nikolić, Aleksandra
PY  - 2017
UR  - http://rik.mrizp.rs/handle/123456789/680
AB  - Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - DNA and biochemical analysis of a potential opaque2 maize population
VL  - 49
IS  - 1
SP  - 51
EP  - 61
DO  - 10.2298/GENSR1701051I
UR  - conv_956
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Nikolić, Ana and Kostadinović, Marija and Nikolić, Aleksandra",
year = "2017",
abstract = "Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "DNA and biochemical analysis of a potential opaque2 maize population",
volume = "49",
number = "1",
pages = "51-61",
doi = "10.2298/GENSR1701051I",
url = "conv_956"
}
Ignjatović-Micić, D., Nikolić, A., Kostadinović, M.,& Nikolić, A.. (2017). DNA and biochemical analysis of a potential opaque2 maize population. in Genetika
Društvo genetičara Srbije, Beograd., 49(1), 51-61.
https://doi.org/10.2298/GENSR1701051I
conv_956
Ignjatović-Micić D, Nikolić A, Kostadinović M, Nikolić A. DNA and biochemical analysis of a potential opaque2 maize population. in Genetika. 2017;49(1):51-61.
doi:10.2298/GENSR1701051I
conv_956 .
Ignjatović-Micić, Dragana, Nikolić, Ana, Kostadinović, Marija, Nikolić, Aleksandra, "DNA and biochemical analysis of a potential opaque2 maize population" in Genetika, 49, no. 1 (2017):51-61,
https://doi.org/10.2298/GENSR1701051I .,
conv_956 .

Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits

Kostadinović, Marija; Ignjatović-Micić, Dragana; Ristić, Danijela; Mladenović-Drinić, Snežana; Vančetović, Jelena; Božinović, Sofija; Stanković, Goran

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Kostadinović, Marija
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Mladenović-Drinić, Snežana
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Stanković, Goran
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/621
AB  - Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.
PB  - Public Library Science, San Francisco
T2  - PLOS ONE
T1  - Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167635
UR  - conv_941
ER  - 
@article{
author = "Kostadinović, Marija and Ignjatović-Micić, Dragana and Ristić, Danijela and Mladenović-Drinić, Snežana and Vančetović, Jelena and Božinović, Sofija and Stanković, Goran",
year = "2016",
abstract = "Breeding program aimed at converting standard maize inbred lines to their quality protein maize (QPM) counterparts for growing in temperate climate is being conducted at Maize Research Institute (MRI). The objective of the research presented herein was to develop QPM versions of two commercial ZP inbreds through marker assisted selection (MAS) with opaque2 specific molecular markers, while maintaining their good agronomic performances and combining abilities. Donor line was a tropical QPM line CML 144. After two backcross and three selfing generations, six near isogenic lines (NILs) with 93% recovery of the recurrent parent genome were created from one cross. Average increments of 30% in tryptophan content and 36% in quality index were obtained, as well as kernels with less than 25% opaque endosperm. Grain yield was increased by 11-31% and combining abilities of the improved lines were on a par with the original line. Correlations between biochemical and agronomic parameters revealed that selection for plant height, ear length and kernel row number together with tryptophan content could be recommended for development of QPM with this material. However, several impediments emerged during selection. Major drawbacks in NIL development were small number of opaque2 recessive homozygotes (4.5% and 7.6% in BC2F2 of two crosses) and poor seed set throughout selection, which led to the loss of one cross. Moreover, in the other cross many plants in different generations had to be omitted from further selection due to the insufficient number of kernels. This phenomenon could be explained by incompatibility between pollen and style, possibly due to the exotic donor germplasm. Overall, it could be expected that the use of NILs, which are adapted to temperate climate and have high percentage of domestic germplasm, would outbalance the noted impediments and increase MAS efficiency in different breeding programs.",
publisher = "Public Library Science, San Francisco",
journal = "PLOS ONE",
title = "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167635",
url = "conv_941"
}
Kostadinović, M., Ignjatović-Micić, D., Ristić, D., Mladenović-Drinić, S., Vančetović, J., Božinović, S.,& Stanković, G.. (2016). Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167635
conv_941
Kostadinović M, Ignjatović-Micić D, Ristić D, Mladenović-Drinić S, Vančetović J, Božinović S, Stanković G. Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits. in PLOS ONE. 2016;11(12).
doi:10.1371/journal.pone.0167635
conv_941 .
Kostadinović, Marija, Ignjatović-Micić, Dragana, Ristić, Danijela, Mladenović-Drinić, Snežana, Vančetović, Jelena, Božinović, Sofija, Stanković, Goran, "Development of High Tryptophan Maize Near Isogenic Lines Adapted to Temperate Regions through Marker Assisted Selection Impediments and Benefits" in PLOS ONE, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167635 .,
conv_941 .
1
7
7
7

Nenad Delić: Genotype by environment interaction in maize breeding, Institut za kukuruz 'Zemun Polje', Beograd, 2015

Ignjatović-Micić, Dragana

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2016)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
PY  - 2016
UR  - http://rik.mrizp.rs/handle/123456789/641
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Nenad Delić: Genotype by environment interaction in maize breeding, Institut za kukuruz 'Zemun Polje', Beograd, 2015
VL  - 22
IS  - 2
SP  - 80
EP  - 80
UR  - conv_309
ER  - 
@article{
author = "Ignjatović-Micić, Dragana",
year = "2016",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Nenad Delić: Genotype by environment interaction in maize breeding, Institut za kukuruz 'Zemun Polje', Beograd, 2015",
volume = "22",
number = "2",
pages = "80-80",
url = "conv_309"
}
Ignjatović-Micić, D.. (2016). Nenad Delić: Genotype by environment interaction in maize breeding, Institut za kukuruz 'Zemun Polje', Beograd, 2015. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 22(2), 80-80.
conv_309
Ignjatović-Micić D. Nenad Delić: Genotype by environment interaction in maize breeding, Institut za kukuruz 'Zemun Polje', Beograd, 2015. in Selekcija i semenarstvo. 2016;22(2):80-80.
conv_309 .
Ignjatović-Micić, Dragana, "Nenad Delić: Genotype by environment interaction in maize breeding, Institut za kukuruz 'Zemun Polje', Beograd, 2015" in Selekcija i semenarstvo, 22, no. 2 (2016):80-80,
conv_309 .

A simple SSR analysis for genetic diversity estimation of maize landraces

Ignjatović-Micić, Dragana; Ristić, Danijela; Babić, Vojka; Anđelković, Violeta; Vančetović, Jelena

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Ignjatović-Micić, Dragana
AU  - Ristić, Danijela
AU  - Babić, Vojka
AU  - Anđelković, Violeta
AU  - Vančetović, Jelena
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/567
AB  - A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11-0.38) than between dent (0.22 - 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - A simple SSR analysis for genetic diversity estimation of maize landraces
VL  - 47
IS  - 1
SP  - 53
EP  - 62
DO  - 10.2298/GENSR1501053I
UR  - conv_905
ER  - 
@article{
author = "Ignjatović-Micić, Dragana and Ristić, Danijela and Babić, Vojka and Anđelković, Violeta and Vančetović, Jelena",
year = "2015",
abstract = "A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11-0.38) than between dent (0.22 - 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "A simple SSR analysis for genetic diversity estimation of maize landraces",
volume = "47",
number = "1",
pages = "53-62",
doi = "10.2298/GENSR1501053I",
url = "conv_905"
}
Ignjatović-Micić, D., Ristić, D., Babić, V., Anđelković, V.,& Vančetović, J.. (2015). A simple SSR analysis for genetic diversity estimation of maize landraces. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 53-62.
https://doi.org/10.2298/GENSR1501053I
conv_905
Ignjatović-Micić D, Ristić D, Babić V, Anđelković V, Vančetović J. A simple SSR analysis for genetic diversity estimation of maize landraces. in Genetika. 2015;47(1):53-62.
doi:10.2298/GENSR1501053I
conv_905 .
Ignjatović-Micić, Dragana, Ristić, Danijela, Babić, Vojka, Anđelković, Violeta, Vančetović, Jelena, "A simple SSR analysis for genetic diversity estimation of maize landraces" in Genetika, 47, no. 1 (2015):53-62,
https://doi.org/10.2298/GENSR1501053I .,
conv_905 .
8
7
10

Effects of alkaline cooking and sprouting on bioactive compounds, their bioavailability and relation to antioxidant capacity of maize flour

Žilić, Slađana; Janković, Marijana; Delić, Nenad; Vančetović, Jelena; Ignjatović-Micić, Dragana; Basić, Zorica

(Vup Food Research Inst, Bratislava, Bratislava 26, 2015)

TY  - JOUR
AU  - Žilić, Slađana
AU  - Janković, Marijana
AU  - Delić, Nenad
AU  - Vančetović, Jelena
AU  - Ignjatović-Micić, Dragana
AU  - Basić, Zorica
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/590
AB  - The contents of total and available niacin, carotenoids, tocopherols, as well as soluble free, conjugated and insoluble bound phenolic compounds was determined in untreated, alkaline-cooked and sprouted kernels of white, sweet and yellow maize. Antioxidant capacity of untreated maize kernels and processed flours was evaluated, too. There was a considerable increase in soluble free and conjugated phenolic compounds contents of maize flour after alkaline cooking and sprouting. The content of total soluble free and conjugated phenolics in maize masa flour and sprouted flour was increased by 35% to 56% and by 46% to 92% as compared to the contents originally present in untreated kernels, respectively. Contrary, conditions of alkaline cooking had strong effect on the loss of bound phenolic compounds, as well as niacin, carotenoids and tocopherols in maize flour. Despite of this, our findings imply that both alkaline cooking and sprouting enhance the antioxidant capacity of maize kernels. According to our results, sprouting is a more effective method to release bound phenolics as well as to enhance the nutritional and functional value of the maize flour. Therefore, sprouted maize, as a rich source of bioavailable phytochemicals, can be used to develop functional maize-based foods.
PB  - Vup Food Research Inst, Bratislava, Bratislava 26
T2  - Journal of Food and Nutrition Research
T1  - Effects of alkaline cooking and sprouting on bioactive compounds, their bioavailability and relation to antioxidant capacity of maize flour
VL  - 54
IS  - 2
SP  - 155
EP  - 164
UR  - conv_911
ER  - 
@article{
author = "Žilić, Slađana and Janković, Marijana and Delić, Nenad and Vančetović, Jelena and Ignjatović-Micić, Dragana and Basić, Zorica",
year = "2015",
abstract = "The contents of total and available niacin, carotenoids, tocopherols, as well as soluble free, conjugated and insoluble bound phenolic compounds was determined in untreated, alkaline-cooked and sprouted kernels of white, sweet and yellow maize. Antioxidant capacity of untreated maize kernels and processed flours was evaluated, too. There was a considerable increase in soluble free and conjugated phenolic compounds contents of maize flour after alkaline cooking and sprouting. The content of total soluble free and conjugated phenolics in maize masa flour and sprouted flour was increased by 35% to 56% and by 46% to 92% as compared to the contents originally present in untreated kernels, respectively. Contrary, conditions of alkaline cooking had strong effect on the loss of bound phenolic compounds, as well as niacin, carotenoids and tocopherols in maize flour. Despite of this, our findings imply that both alkaline cooking and sprouting enhance the antioxidant capacity of maize kernels. According to our results, sprouting is a more effective method to release bound phenolics as well as to enhance the nutritional and functional value of the maize flour. Therefore, sprouted maize, as a rich source of bioavailable phytochemicals, can be used to develop functional maize-based foods.",
publisher = "Vup Food Research Inst, Bratislava, Bratislava 26",
journal = "Journal of Food and Nutrition Research",
title = "Effects of alkaline cooking and sprouting on bioactive compounds, their bioavailability and relation to antioxidant capacity of maize flour",
volume = "54",
number = "2",
pages = "155-164",
url = "conv_911"
}
Žilić, S., Janković, M., Delić, N., Vančetović, J., Ignjatović-Micić, D.,& Basić, Z.. (2015). Effects of alkaline cooking and sprouting on bioactive compounds, their bioavailability and relation to antioxidant capacity of maize flour. in Journal of Food and Nutrition Research
Vup Food Research Inst, Bratislava, Bratislava 26., 54(2), 155-164.
conv_911
Žilić S, Janković M, Delić N, Vančetović J, Ignjatović-Micić D, Basić Z. Effects of alkaline cooking and sprouting on bioactive compounds, their bioavailability and relation to antioxidant capacity of maize flour. in Journal of Food and Nutrition Research. 2015;54(2):155-164.
conv_911 .
Žilić, Slađana, Janković, Marijana, Delić, Nenad, Vančetović, Jelena, Ignjatović-Micić, Dragana, Basić, Zorica, "Effects of alkaline cooking and sprouting on bioactive compounds, their bioavailability and relation to antioxidant capacity of maize flour" in Journal of Food and Nutrition Research, 54, no. 2 (2015):155-164,
conv_911 .
5
8

Genetic diversity of maize inbred lines as inferred from SSR markers

Nikolić, Ana; Ignjatović-Micić, Dragana; Kovačević, Dragan; Čamdžija, Zoran; Filipović, Milomir; Mladenović-Drinić, Snežana

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Nikolić, Ana
AU  - Ignjatović-Micić, Dragana
AU  - Kovačević, Dragan
AU  - Čamdžija, Zoran
AU  - Filipović, Milomir
AU  - Mladenović-Drinić, Snežana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/585
AB  - Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and new problems related to different biotic and abiotic stresses and the growing needs of the world market require constant work on finding new ways for advancing maize production. Molecular marker technology is one of the fastest developing fields and its implementation has already given results in solving different problems related to maize breeding improvement. The aim of the study presented herein was characterization and genetic similarity assessment of twenty-nine maize inbred lines from Maize Research Institute collection using Simple Sequence Repeats (SSR) markers. The analysis was done using 20 pairs of SSR primers with clearly visible and reproducible results. A total of 119 alleles were detected with a mean of 5.8 per locus. PIC (Polymorphism Information Content) values were in the range from 0.45 to 0.92 (average 0.74). Genetic similarities calculated using Jaccard's coefficient ranged from 0.27 to 0.99. Cluster and Principal Component Analysis (PCA) analysis were done using matrices of similarity in the NTSYSpc software, version 2.1. Results of both classifications were moderately in agreement with the pedigree data of analysed genotypes. The information about genetic diversity of maize inbred lines revealed by SSR markers could be useful in planning strategies for future maize breeding programs.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic diversity of maize inbred lines as inferred from SSR markers
VL  - 47
IS  - 2
SP  - 489
EP  - 498
DO  - 10.2298/GENSR1502489N
UR  - conv_916
ER  - 
@article{
author = "Nikolić, Ana and Ignjatović-Micić, Dragana and Kovačević, Dragan and Čamdžija, Zoran and Filipović, Milomir and Mladenović-Drinić, Snežana",
year = "2015",
abstract = "Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and new problems related to different biotic and abiotic stresses and the growing needs of the world market require constant work on finding new ways for advancing maize production. Molecular marker technology is one of the fastest developing fields and its implementation has already given results in solving different problems related to maize breeding improvement. The aim of the study presented herein was characterization and genetic similarity assessment of twenty-nine maize inbred lines from Maize Research Institute collection using Simple Sequence Repeats (SSR) markers. The analysis was done using 20 pairs of SSR primers with clearly visible and reproducible results. A total of 119 alleles were detected with a mean of 5.8 per locus. PIC (Polymorphism Information Content) values were in the range from 0.45 to 0.92 (average 0.74). Genetic similarities calculated using Jaccard's coefficient ranged from 0.27 to 0.99. Cluster and Principal Component Analysis (PCA) analysis were done using matrices of similarity in the NTSYSpc software, version 2.1. Results of both classifications were moderately in agreement with the pedigree data of analysed genotypes. The information about genetic diversity of maize inbred lines revealed by SSR markers could be useful in planning strategies for future maize breeding programs.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic diversity of maize inbred lines as inferred from SSR markers",
volume = "47",
number = "2",
pages = "489-498",
doi = "10.2298/GENSR1502489N",
url = "conv_916"
}
Nikolić, A., Ignjatović-Micić, D., Kovačević, D., Čamdžija, Z., Filipović, M.,& Mladenović-Drinić, S.. (2015). Genetic diversity of maize inbred lines as inferred from SSR markers. in Genetika
Društvo genetičara Srbije, Beograd., 47(2), 489-498.
https://doi.org/10.2298/GENSR1502489N
conv_916
Nikolić A, Ignjatović-Micić D, Kovačević D, Čamdžija Z, Filipović M, Mladenović-Drinić S. Genetic diversity of maize inbred lines as inferred from SSR markers. in Genetika. 2015;47(2):489-498.
doi:10.2298/GENSR1502489N
conv_916 .
Nikolić, Ana, Ignjatović-Micić, Dragana, Kovačević, Dragan, Čamdžija, Zoran, Filipović, Milomir, Mladenović-Drinić, Snežana, "Genetic diversity of maize inbred lines as inferred from SSR markers" in Genetika, 47, no. 2 (2015):489-498,
https://doi.org/10.2298/GENSR1502489N .,
conv_916 .
1
5
5

A diallel cross among drought tolerant maize populations

Vančetović, Jelena; Božinović, Sofija; Ignjatović-Micić, Dragana; Delić, Nenad; Kravić, Natalija; Nikolić, Ana

(Springer, Dordrecht, 2015)

TY  - JOUR
AU  - Vančetović, Jelena
AU  - Božinović, Sofija
AU  - Ignjatović-Micić, Dragana
AU  - Delić, Nenad
AU  - Kravić, Natalija
AU  - Nikolić, Ana
PY  - 2015
UR  - http://rik.mrizp.rs/handle/123456789/587
AB  - A complete diallel without reciprocals was investigated among six drought tolerant maize (Zea mays L.) landraces from MRI Zemun Polje gene bank. Trials with parental populations, their crosses and three check hybrids were conducted at three locations in 2012 and five locations in 2013 in Serbia. The aim was to determine potential heterotic patterns for reciprocal recurrent selection (RRS) among them. The average grain yield reached 4.832 and 5.864 t ha(-1) for the populations and population crosses, respectively. Mipdarent heterosis (MPH) for grain yield ranged from 10.0 to 36.7 %, with the average value of 21.3 %. Entry x environment interactions did not show significance for any trait, reflecting a broader adaptation of the material to different environments. The new heterotic pattern proposed for RRS was Iranian dent POP. N-425 x Argentinean flint ARZM 06-020, which had the highest grain yield (6.871 t ha(-1)) and MPH value (36.7 %). This cross confirms the hypotesis that broader geographical origin, as well as dent x flint type of cross, is in positive correlation with high MPH for grain yield in maize. The cluster analysis based on MPH was not in accordance with the single sequence repeat (SSR) analysis of parental populations. Since both of the populations have some undesirable agronomic traits (high moisture, root and stalk lodging, bareness), two to three cycles of intrapopulation selection for improving them before starting the RRS program should be performed. In some additional grain quality research, these populations showed favorable value-added traits (high tryptophan, oil, and saturated fatty acids), indicating the possibility of improving grain quality besides grain yield in this newly discovered heterotic pattern.
PB  - Springer, Dordrecht
T2  - Euphytica
T1  - A diallel cross among drought tolerant maize populations
VL  - 205
IS  - 1
SP  - 1
EP  - 16
DO  - 10.1007/s10681-015-1372-9
UR  - conv_914
ER  - 
@article{
author = "Vančetović, Jelena and Božinović, Sofija and Ignjatović-Micić, Dragana and Delić, Nenad and Kravić, Natalija and Nikolić, Ana",
year = "2015",
abstract = "A complete diallel without reciprocals was investigated among six drought tolerant maize (Zea mays L.) landraces from MRI Zemun Polje gene bank. Trials with parental populations, their crosses and three check hybrids were conducted at three locations in 2012 and five locations in 2013 in Serbia. The aim was to determine potential heterotic patterns for reciprocal recurrent selection (RRS) among them. The average grain yield reached 4.832 and 5.864 t ha(-1) for the populations and population crosses, respectively. Mipdarent heterosis (MPH) for grain yield ranged from 10.0 to 36.7 %, with the average value of 21.3 %. Entry x environment interactions did not show significance for any trait, reflecting a broader adaptation of the material to different environments. The new heterotic pattern proposed for RRS was Iranian dent POP. N-425 x Argentinean flint ARZM 06-020, which had the highest grain yield (6.871 t ha(-1)) and MPH value (36.7 %). This cross confirms the hypotesis that broader geographical origin, as well as dent x flint type of cross, is in positive correlation with high MPH for grain yield in maize. The cluster analysis based on MPH was not in accordance with the single sequence repeat (SSR) analysis of parental populations. Since both of the populations have some undesirable agronomic traits (high moisture, root and stalk lodging, bareness), two to three cycles of intrapopulation selection for improving them before starting the RRS program should be performed. In some additional grain quality research, these populations showed favorable value-added traits (high tryptophan, oil, and saturated fatty acids), indicating the possibility of improving grain quality besides grain yield in this newly discovered heterotic pattern.",
publisher = "Springer, Dordrecht",
journal = "Euphytica",
title = "A diallel cross among drought tolerant maize populations",
volume = "205",
number = "1",
pages = "1-16",
doi = "10.1007/s10681-015-1372-9",
url = "conv_914"
}
Vančetović, J., Božinović, S., Ignjatović-Micić, D., Delić, N., Kravić, N.,& Nikolić, A.. (2015). A diallel cross among drought tolerant maize populations. in Euphytica
Springer, Dordrecht., 205(1), 1-16.
https://doi.org/10.1007/s10681-015-1372-9
conv_914
Vančetović J, Božinović S, Ignjatović-Micić D, Delić N, Kravić N, Nikolić A. A diallel cross among drought tolerant maize populations. in Euphytica. 2015;205(1):1-16.
doi:10.1007/s10681-015-1372-9
conv_914 .
Vančetović, Jelena, Božinović, Sofija, Ignjatović-Micić, Dragana, Delić, Nenad, Kravić, Natalija, Nikolić, Ana, "A diallel cross among drought tolerant maize populations" in Euphytica, 205, no. 1 (2015):1-16,
https://doi.org/10.1007/s10681-015-1372-9 .,
conv_914 .
6
13
14