TY  - JOUR
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Stanković, Slavica
AU  - Milivojević, Marija
AU  - Vukadinović, Radmila
AU  - Stepanić, Ana
PY  - 2013
UR  - http://rik.mrizp.rs/handle/123456789/478
AB  - The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - Detection of Rhizomucor pusillus on sunflower seed
VL  - 36
IS  - 59
SP  - 59
EP  - 70
DO  - 10.2298/HEL1359059L
ER  - 

@article{
author = "Lević, Jelena and Ivanović, D. and Stanković, Slavica and Milivojević, Marija and Vukadinović, Radmila and Stepanić, Ana",
year = "2013",
abstract = "The accelerated ageing test method (AA), agar plate method (A) and blotter method (B) have been used to detect the Rhizomucor pusillus and other mycobita on 24 samples of sunflower seed. Sterilised and unsterilised sunflower seeds have been incubated at 25ºC and 42ºC in the dark for 72 and 144 hours. The fungus was not detected in any sample at 25ºC, not even after 144 h incubation of seeds. The fungal frequency ranged from 58.3 (B method) to 75.0% (A method) and from 4.2% (B method) to 16.7% (AA method) after 72 h incubation of unsterilised and sterilised samples at 42ºC, respectively. The fungal incidence was 25.5% (AA method), 21.9% (A method) and 20.3% (B method) after 72 h incubation of unsterilised seed, and 2% on sterilised seed in all three applied methods. By extended incubation of unsterilised and sterilised seeds up to 144 h at 42°C the frequency and incidence of the fungus did not significantly change. The results of the present research show that the AA test method, widely applied in seed longevity testing, can be used as a simple and efficient method for the detection of R. pusillus as well as other mycobiota on sunflower seed. Furthermore, these are the first data on determining the presence of R. pusillus in Serbia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "Detection of Rhizomucor pusillus on sunflower seed",
volume = "36",
number = "59",
pages = "59-70",
doi = "10.2298/HEL1359059L"
}

Lević, J., Ivanović, D., Stanković, S., Milivojević, M., Vukadinović, R.,& Stepanić, A.. (2013). Detection of Rhizomucor pusillus on sunflower seed. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 36(59), 59-70.
https://doi.org/10.2298/HEL1359059L

Lević J, Ivanović D, Stanković S, Milivojević M, Vukadinović R, Stepanić A. Detection of Rhizomucor pusillus on sunflower seed. in Helia. 2013;36(59):59-70.
doi:10.2298/HEL1359059L .

Lević, Jelena, Ivanović, D., Stanković, Slavica, Milivojević, Marija, Vukadinović, Radmila, Stepanić, Ana, "Detection of Rhizomucor pusillus on sunflower seed" in Helia, 36, no. 59 (2013):59-70,
https://doi.org/10.2298/HEL1359059L . .

TY  - JOUR
AU  - Župunski, Vesna
AU  - Jevtić, Radivoje
AU  - Ignjatović-Micić, Dragana
AU  - Stanković, Slavica
AU  - Lević, Jelena
AU  - Ivanović, D.
AU  - Nikolić, Ana
PY  - 2012
UR  - http://rik.mrizp.rs/handle/123456789/414
AB  - With the growing importance of organic production and resurgence of bunt fungi in organic farming conditions, the present study was undertaken to determine incidence and contamination level of Tilletia species in conventionally produced non-processed seed of wheat in Vojvodina. Analysis of 151 samples of basic, certified and commercial seed revealed that 129 samples were contaminated with Tilletia species. The predominant species was T. caries, which was found in 127 samples. However, teliospores of Tilletia species, which have not been registered in Serbia before, were found in five samples. These teliospores had a prominent gelatinous sheath together with conspicuous, deep reticulation and it was assumed that some of them might be of the quarantined T. contraversa, whilst others were identified as T. bromi. Contamination level of basic and certified seed was about one teliospore per 10 seeds, while four out of 16 commercial seed samples were contaminated with more than 900 teliospores per seed which exceeds the allowed contamination level.
PB  - Ista-Int Seed Testing Assoc, Bassersdorf
T2  - Seed Science and Technology
T1  - Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia
VL  - 40
IS  - 3
SP  - 320
EP  - 332
DO  - 10.15258/sst.2012.40.3.04
ER  - 

@article{
author = "Župunski, Vesna and Jevtić, Radivoje and Ignjatović-Micić, Dragana and Stanković, Slavica and Lević, Jelena and Ivanović, D. and Nikolić, Ana",
year = "2012",
abstract = "With the growing importance of organic production and resurgence of bunt fungi in organic farming conditions, the present study was undertaken to determine incidence and contamination level of Tilletia species in conventionally produced non-processed seed of wheat in Vojvodina. Analysis of 151 samples of basic, certified and commercial seed revealed that 129 samples were contaminated with Tilletia species. The predominant species was T. caries, which was found in 127 samples. However, teliospores of Tilletia species, which have not been registered in Serbia before, were found in five samples. These teliospores had a prominent gelatinous sheath together with conspicuous, deep reticulation and it was assumed that some of them might be of the quarantined T. contraversa, whilst others were identified as T. bromi. Contamination level of basic and certified seed was about one teliospore per 10 seeds, while four out of 16 commercial seed samples were contaminated with more than 900 teliospores per seed which exceeds the allowed contamination level.",
publisher = "Ista-Int Seed Testing Assoc, Bassersdorf",
journal = "Seed Science and Technology",
title = "Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia",
volume = "40",
number = "3",
pages = "320-332",
doi = "10.15258/sst.2012.40.3.04"
}

Župunski, V., Jevtić, R., Ignjatović-Micić, D., Stanković, S., Lević, J., Ivanović, D.,& Nikolić, A.. (2012). Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia. in Seed Science and Technology
Ista-Int Seed Testing Assoc, Bassersdorf., 40(3), 320-332.
https://doi.org/10.15258/sst.2012.40.3.04

Župunski V, Jevtić R, Ignjatović-Micić D, Stanković S, Lević J, Ivanović D, Nikolić A. Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia. in Seed Science and Technology. 2012;40(3):320-332.
doi:10.15258/sst.2012.40.3.04 .

Župunski, Vesna, Jevtić, Radivoje, Ignjatović-Micić, Dragana, Stanković, Slavica, Lević, Jelena, Ivanović, D., Nikolić, Ana, "Incidence of Tilletia species in non-processed seed of Triticum aestivum in Vojvodina, Serbia" in Seed Science and Technology, 40, no. 3 (2012):320-332,
https://doi.org/10.15258/sst.2012.40.3.04 . .