@conference{
author = "Banović Đeri, Bojana and Dudić, Dragana and Božić, Manja and Janić, Pavle and Džodanović, Lana and Samardžić, Jelena and Ignjatović-Micić, Dragana and Vančetović, Jelena and Božinović, Sofija and Dodig, Dejan and Delić, Nenad and Nikolić, Ana",
year = "2019",
abstract = "Abiotic stresses cause great losses in crop production worldwide. Due to climatic changes
temperatures higher than the average are frequently observed in temperate areas in the
course of maize flowering and grain filling period, displaying detrimental effect on maize yield.
In order to overcome this problem development of drought tolerant maize lines, but also
cold tolerant maize lines that would allow earlier sowing and hence avoidance of extremely
high temperatures during sensitive developmental phases in maize, became important goal
of maize breeding programs in temperate areas. A set of 46 maize inbred lines was chosen
from three main heterotic groups (BSSS, Lancaster, Iowa dent) important for commercial
breeding programs. Plants were grown under the optimal conditions in the greenhouse and
sampled upon entering V5 growth stage. A total RNA isolated from leaves of three plants of
each inbred line was used for DNA library preparation by Illumina TruSeq Stranded RNA LT
kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent
kit v2 (2x150bp). For data manipulation and analysis a custom made bioinformatics pipeline
was used. Differential gene expression analysis revealed a minimum of 21 genes that were
strongly statistically supported for differential expression between the inbred lines and also
annotated as involved in abiotic stress responses in other plant species. We tested 10 of
these genes in maize by using qPCR on the subset of 8 inbred lines grown under optimal, cold
and drought conditions. Real-time PCR results revealed significant change in the expression
level of tested genes under abiotic stresses, supporting bioinformatics prediction. Verified
genes were then submitted to free online gene networking tools and after determining
relationships between predicted networks and inbred line phenotypes, possible novel gene
candidates for enriching maize abiotic stress signaling network resolution were proposed.",
publisher = "Belgrade : Serbian Genetics Society",
journal = "6. Congress of the Serbian Genetic Society, 14-17 October, 2019, Vrnjačka Banja, Serbia - Book of Abstracts",
title = "Leaf total transcriptome analysis enriches insight into maize abiotic stress signaling network",
pages = "260-260",
url = "https://hdl.handle.net/21.15107/rcub_rik_1126"
}
