Slatinskaya, O. V.

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  • Slatinskaya, O. V. (2)
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Photosynthetic pigments and phytochemical activity of photosynthetic apparatus of maize (Zea mays L.) leaves under the effect of thiamethoxam

Todorenko, D. A.; Slatinskaya, O. V.; Hao, J.; Seifullina, N. Kh.; Radenović, Čedomir; Matorin, D. N.; Maksimov, G. V.

(Moscow : Russian Academy of Agricultural Sciences, 2020)

TY  - JOUR
AU  - Todorenko, D. A.
AU  - Slatinskaya, O. V.
AU  - Hao, J.
AU  - Seifullina, N. Kh.
AU  - Radenović, Čedomir
AU  - Matorin, D. N.
AU  - Maksimov, G. V.
PY  - 2020
UR  - http://rik.mrizp.rs/handle/123456789/794
AB  - In the last decade, neonicotinoid insecticides have been actively used to protect plants from pests. Moreover, their effect on the plants, in particular on the state of photosynthetic pig-ments, has been studied paucity. In the present work, it was shown for the first time that treatment of maize (Zea maysL.) leaves with a thiamethoxam (TMX) insecticide leads to a decrease in the functional activity of photosystem II and a decrease in the energyzation of thylakoid membranes. In addition, the effect of thiamethoxam depends on the genotype of maize. The aim of the work was to study the effect of thiamethoxam pesticide on photosynthetic pigments and the photochemical activi-ty of the photosynthetic apparatus of maize leaves of two genotypes. The experiments were carried out in 2018-2019.The object of the study was samples of maize leaves of the inbred line zppl 225 and  hybrid  zp  341  with  high  rates  of  germination,  grain  quality  and  yield  (Institut  za  kukuruz “Zemun Polje”, Belgrade, Serbia). The seeds were germinated until the roots appeared (length not less than 5mm),after which they were planted in the soil (vermiculite:chernozem mixture, 1:1) and grown under 16-hour daylight at a constant temperature of 25 С. When the third trueleaf appeared (more than 4 cm in length), the plants were sprayed with a TMX solution at a concentration of 0.2 mg/l.  Plants  grown  under  similar  conditions  without  TMX  spraying  were  used  as  controls.  The measurements were carried out when the fifth true leaf reached a size of 12-14 cm.The content of photosynthetic pigments (chlorophyll a, b and carotenoids) was determined spectrophotometrically in 100% acetone extract and calculated using the Holm-Wetstein formula. A change in the confor-mation of carotenoid molecules was recorded by Raman spectroscopy.Light-induced kinetics of prompt fluorescence (PF), delayed fluorescence (DF) and modulated reflection at = 820 nm (MR) were recorded simultaneously using a multifunctional plant efficiency analyzer M-PEA-2 (Hansatech Instruments, Great Britain). PF induction curves (OJIP curves) were analyzed using a standard JIP-test. It was found that in the phase of the fifth true leaf, in the inbred line zppl 225, the chlorophyll content in the presence of TMX decreased from 0.74 to 0.61 mg/g: the amount of chlorophyll a decreased by 17 %, chlorophyll b by 24 %. In contrast, no changes in pigment composition were detected in the leaves of the zp 341 hybrid when exposed to TMX. The OJIP curves of the control and TMX-treated leaves had a typical curve with characteristic O-J, J-I, and I-P phases, which re-flected the processes of sequential reduction of carriers in the electron transport chain of photosyn-thesis (ETC) between two photosystems. The effect of the pesticide onthe leaves of two maize geno-types was manifested in a decrease in the functional state of photosystem II, determined by the fluo-rescence parameter (PIABS), which was obtained based on the analysis of OJIP curves using the JIP-test. Comparison of PIABSin the control and under the influence of TMX revealed statistically sig-
67nificant (p < 0.05) differences: in the leaves of zppl 225 and zp 341 samples treated with TMX, the PIABSparameter decreased by 29 and 24%, respectively. Changes in the fast phase of delayed fluo-rescence, associated with a decrease in the energyzation of the thylakoid membrane upon exposure to TMX, were detected in the leaves of maize. An analysis of the maximum oxidation and reduction rates of P700 (MR kinetics) indicates a decrease in the acceptor pool on the acceptor side of PSI in zppl 225 leaves when exposed to TMX. It was found that the reaction centers (RCs) of PSI zp 341 showed resistance to TMX (no change in the redox transformations of P700). TMX caused changes in the conformation of carotenoid molecules, but did not change their content in the leaf. The pro-posed combination of methods for prompt fluorescence, delayed fluorescence, modulated reflection at = 820 nm and Raman spectroscopy can be the basis for the formation of an effective technology for the diagnosis of early defects of photosynthetic pigments when pesticides enter an intact plant.
PB  - Moscow : Russian Academy of Agricultural Sciences
T2  - Agricultural biology
T1  - Photosynthetic pigments and phytochemical activity of photosynthetic apparatus of maize (Zea mays L.) leaves under the effect of thiamethoxam
VL  - 55
IS  - 1
SP  - 66
EP  - 76
DO  - 10.15389/agrobiology.2020.1.66eng
ER  - 
@article{
author = "Todorenko, D. A. and Slatinskaya, O. V. and Hao, J. and Seifullina, N. Kh. and Radenović, Čedomir and Matorin, D. N. and Maksimov, G. V.",
year = "2020",
url = "http://rik.mrizp.rs/handle/123456789/794",
abstract = "In the last decade, neonicotinoid insecticides have been actively used to protect plants from pests. Moreover, their effect on the plants, in particular on the state of photosynthetic pig-ments, has been studied paucity. In the present work, it was shown for the first time that treatment of maize (Zea maysL.) leaves with a thiamethoxam (TMX) insecticide leads to a decrease in the functional activity of photosystem II and a decrease in the energyzation of thylakoid membranes. In addition, the effect of thiamethoxam depends on the genotype of maize. The aim of the work was to study the effect of thiamethoxam pesticide on photosynthetic pigments and the photochemical activi-ty of the photosynthetic apparatus of maize leaves of two genotypes. The experiments were carried out in 2018-2019.The object of the study was samples of maize leaves of the inbred line zppl 225 and  hybrid  zp  341  with  high  rates  of  germination,  grain  quality  and  yield  (Institut  za  kukuruz “Zemun Polje”, Belgrade, Serbia). The seeds were germinated until the roots appeared (length not less than 5mm),after which they were planted in the soil (vermiculite:chernozem mixture, 1:1) and grown under 16-hour daylight at a constant temperature of 25 С. When the third trueleaf appeared (more than 4 cm in length), the plants were sprayed with a TMX solution at a concentration of 0.2 mg/l.  Plants  grown  under  similar  conditions  without  TMX  spraying  were  used  as  controls.  The measurements were carried out when the fifth true leaf reached a size of 12-14 cm.The content of photosynthetic pigments (chlorophyll a, b and carotenoids) was determined spectrophotometrically in 100% acetone extract and calculated using the Holm-Wetstein formula. A change in the confor-mation of carotenoid molecules was recorded by Raman spectroscopy.Light-induced kinetics of prompt fluorescence (PF), delayed fluorescence (DF) and modulated reflection at = 820 nm (MR) were recorded simultaneously using a multifunctional plant efficiency analyzer M-PEA-2 (Hansatech Instruments, Great Britain). PF induction curves (OJIP curves) were analyzed using a standard JIP-test. It was found that in the phase of the fifth true leaf, in the inbred line zppl 225, the chlorophyll content in the presence of TMX decreased from 0.74 to 0.61 mg/g: the amount of chlorophyll a decreased by 17 %, chlorophyll b by 24 %. In contrast, no changes in pigment composition were detected in the leaves of the zp 341 hybrid when exposed to TMX. The OJIP curves of the control and TMX-treated leaves had a typical curve with characteristic O-J, J-I, and I-P phases, which re-flected the processes of sequential reduction of carriers in the electron transport chain of photosyn-thesis (ETC) between two photosystems. The effect of the pesticide onthe leaves of two maize geno-types was manifested in a decrease in the functional state of photosystem II, determined by the fluo-rescence parameter (PIABS), which was obtained based on the analysis of OJIP curves using the JIP-test. Comparison of PIABSin the control and under the influence of TMX revealed statistically sig-
67nificant (p < 0.05) differences: in the leaves of zppl 225 and zp 341 samples treated with TMX, the PIABSparameter decreased by 29 and 24%, respectively. Changes in the fast phase of delayed fluo-rescence, associated with a decrease in the energyzation of the thylakoid membrane upon exposure to TMX, were detected in the leaves of maize. An analysis of the maximum oxidation and reduction rates of P700 (MR kinetics) indicates a decrease in the acceptor pool on the acceptor side of PSI in zppl 225 leaves when exposed to TMX. It was found that the reaction centers (RCs) of PSI zp 341 showed resistance to TMX (no change in the redox transformations of P700). TMX caused changes in the conformation of carotenoid molecules, but did not change their content in the leaf. The pro-posed combination of methods for prompt fluorescence, delayed fluorescence, modulated reflection at = 820 nm and Raman spectroscopy can be the basis for the formation of an effective technology for the diagnosis of early defects of photosynthetic pigments when pesticides enter an intact plant.",
publisher = "Moscow : Russian Academy of Agricultural Sciences",
journal = "Agricultural biology",
title = "Photosynthetic pigments and phytochemical activity of photosynthetic apparatus of maize (Zea mays L.) leaves under the effect of thiamethoxam",
volume = "55",
number = "1",
pages = "66-76",
doi = "10.15389/agrobiology.2020.1.66eng"
}
Todorenko, D. A., Slatinskaya, O. V., Hao, J., Seifullina, N. Kh., Radenović, Č., Matorin, D. N.,& Maksimov, G. V. (2020). Photosynthetic pigments and phytochemical activity of photosynthetic apparatus of maize (Zea mays L.) leaves under the effect of thiamethoxam.
Agricultural biology
Moscow : Russian Academy of Agricultural Sciences., 55(1), 66-76.
https://doi.org/10.15389/agrobiology.2020.1.66eng
Todorenko DA, Slatinskaya OV, Hao J, Seifullina NK, Radenović Č, Matorin DN, Maksimov GV. Photosynthetic pigments and phytochemical activity of photosynthetic apparatus of maize (Zea mays L.) leaves under the effect of thiamethoxam. Agricultural biology. 2020;55(1):66-76
Todorenko D. A., Slatinskaya O. V., Hao J., Seifullina N. Kh., Radenović Čedomir, Matorin D. N., Maksimov G. V., "Photosynthetic pigments and phytochemical activity of photosynthetic apparatus of maize (Zea mays L.) leaves under the effect of thiamethoxam" Agricultural biology, 55, no. 1 (2020):66-76,
https://doi.org/10.15389/agrobiology.2020.1.66eng .

Complete Study of Nature and Importance of Spectral Bands Contained in Infrared Spectra of Leaves of Maize Inbred Lines with Significant Breeding Properties

Radenović, Čedomir; Maksimov, G. V.; Shutova, V. V.; Slatinskaya, O. V.; Protopopov, F. F.; Delić, Nenad; Grčić, Nikola; Pavlov, Jovan; Čamdžija, Zoran

(Allerton Press, 2019)

TY  - JOUR
AU  - Radenović, Čedomir
AU  - Maksimov, G. V.
AU  - Shutova, V. V.
AU  - Slatinskaya, O. V.
AU  - Protopopov, F. F.
AU  - Delić, Nenad
AU  - Grčić, Nikola
AU  - Pavlov, Jovan
AU  - Čamdžija, Zoran
PY  - 2019
UR  - http://rik.mrizp.rs/handle/123456789/768
AB  - In this article we used the method of IR spectroscopy to identify bioactive organic compounds and
their structural characteristics in the leaves of maize inbred lines ZPPL 186, ZPPL 225 and M1-3-3 Sdms
based on a standard study of high intensity high-amplitude spectral bands and based on attempt-study of
hypothetically influential low intensity low-amplitude spectral bands. The made assumption was that numerous
spectral bands, not analyzed yet, but occurring in different kinetic forms should be systematically analyzed
and the dynamics of their formation caused by different oscillations and vibrations of molecular bonds
of organic compounds and their functional groups should be proven. Low intensity spectral bands imply
unstable state of the biological system (leaf), which is a consequence of the excited state of molecules, radicals,
atoms or ions in tissues, cells or biomembranes. Similar transport processes occur when biological systems
are rhythmically stimulated, as well as in the case of a complex ion transport across the excited thylakoid
membrane. These bands most frequently occurred in the wave number range of 500–1600 cm–1; they
appeared in several spots in wave number ranges up to 3000 cm–1. Amines, primary and secondary amides,
proteins, amino acids, alkanes, alkenes, aldehydes, ketones, aromatic compounds, carbohydrates, carboxylic
acids, ethers, alcohols, sulfides, mono- and polysaccharides were identified in leaves of inbred lines. The
analysis of spectral bands showed that the most intensive was the inbred line ZPPL 186, then the ZPPL M1-3-3
Sdms, while the lowest intensity was detected in ZPPL 225. Thus, by overall study it is possible to identify not
only organic compounds and their structures in leaves of inbred lines, but also to indicate the possibility of
causing an unstable and conformational state in them.
PB  - Allerton Press
T2  - Russian Agricultural Sciences
T1  - Complete Study of Nature and Importance of Spectral Bands Contained in Infrared Spectra of Leaves of Maize Inbred Lines with Significant Breeding Properties
VL  - 45
IS  - 4
SP  - 334
EP  - 339
DO  - 10.3103/S106836741904013X
ER  - 
@article{
author = "Radenović, Čedomir and Maksimov, G. V. and Shutova, V. V. and Slatinskaya, O. V. and Protopopov, F. F. and Delić, Nenad and Grčić, Nikola and Pavlov, Jovan and Čamdžija, Zoran",
year = "2019",
url = "http://rik.mrizp.rs/handle/123456789/768",
abstract = "In this article we used the method of IR spectroscopy to identify bioactive organic compounds and
their structural characteristics in the leaves of maize inbred lines ZPPL 186, ZPPL 225 and M1-3-3 Sdms
based on a standard study of high intensity high-amplitude spectral bands and based on attempt-study of
hypothetically influential low intensity low-amplitude spectral bands. The made assumption was that numerous
spectral bands, not analyzed yet, but occurring in different kinetic forms should be systematically analyzed
and the dynamics of their formation caused by different oscillations and vibrations of molecular bonds
of organic compounds and their functional groups should be proven. Low intensity spectral bands imply
unstable state of the biological system (leaf), which is a consequence of the excited state of molecules, radicals,
atoms or ions in tissues, cells or biomembranes. Similar transport processes occur when biological systems
are rhythmically stimulated, as well as in the case of a complex ion transport across the excited thylakoid
membrane. These bands most frequently occurred in the wave number range of 500–1600 cm–1; they
appeared in several spots in wave number ranges up to 3000 cm–1. Amines, primary and secondary amides,
proteins, amino acids, alkanes, alkenes, aldehydes, ketones, aromatic compounds, carbohydrates, carboxylic
acids, ethers, alcohols, sulfides, mono- and polysaccharides were identified in leaves of inbred lines. The
analysis of spectral bands showed that the most intensive was the inbred line ZPPL 186, then the ZPPL M1-3-3
Sdms, while the lowest intensity was detected in ZPPL 225. Thus, by overall study it is possible to identify not
only organic compounds and their structures in leaves of inbred lines, but also to indicate the possibility of
causing an unstable and conformational state in them.",
publisher = "Allerton Press",
journal = "Russian Agricultural Sciences",
title = "Complete Study of Nature and Importance of Spectral Bands Contained in Infrared Spectra of Leaves of Maize Inbred Lines with Significant Breeding Properties",
volume = "45",
number = "4",
pages = "334-339",
doi = "10.3103/S106836741904013X"
}
Radenović, Č., Maksimov, G. V., Shutova, V. V., Slatinskaya, O. V., Protopopov, F. F., Delić, N., Grčić, N., Pavlov, J.,& Čamdžija, Z. (2019). Complete Study of Nature and Importance of Spectral Bands Contained in Infrared Spectra of Leaves of Maize Inbred Lines with Significant Breeding Properties.
Russian Agricultural Sciences
Allerton Press., 45(4), 334-339.
https://doi.org/10.3103/S106836741904013X
Radenović Č, Maksimov GV, Shutova VV, Slatinskaya OV, Protopopov FF, Delić N, Grčić N, Pavlov J, Čamdžija Z. Complete Study of Nature and Importance of Spectral Bands Contained in Infrared Spectra of Leaves of Maize Inbred Lines with Significant Breeding Properties. Russian Agricultural Sciences. 2019;45(4):334-339
Radenović Čedomir, Maksimov G. V., Shutova V. V., Slatinskaya O. V., Protopopov F. F., Delić Nenad, Grčić Nikola, Pavlov Jovan, Čamdžija Zoran, "Complete Study of Nature and Importance of Spectral Bands Contained in Infrared Spectra of Leaves of Maize Inbred Lines with Significant Breeding Properties" Russian Agricultural Sciences, 45, no. 4 (2019):334-339,
https://doi.org/10.3103/S106836741904013X .